Cefoxitin inhibits the formation of biofilm involved in antimicrobial resistance MDR Escherichia coli.

IF 1.7 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-03-23 DOI:10.1080/10495398.2025.2480176
Hailan Ma, Dacheng Liu, Chen Song, Hongliang Fan, Weiguang Zhou, Hongxia Zhao
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引用次数: 0

Abstract

The study investigates the relationship between biofilm formation and antibiotic resistance in Escherichia coli (E. coli) isolated from calves. Using biochemical and molecular methods, we identified the isolates and assessed their biofilm-forming ability through an improved crystal violet staining method. The minimum inhibitory concentrations (MICs) of 18 antibiotics against the isolates were determined using the broth microdilution method. The impact of cefoxitin on biofilm formation was analyzed using laser scanning confocal microscopy (LSCM). Additionally, qRT-PCR was employed to evaluate the expression levels of biofilm-related genes (luxS, motA, fliA, pfs, and csgD) in response to varying cefoxitin concentrations. Results indicated a significant correlation between antimicrobial resistance (AMR) and biofilm formation ability. Cefoxitin effectively reduced biofilm formation of multidrug-resistant E. coli isolates at 1/2 and 1 MIC, with enhanced inhibition at higher concentrations. The QS-related genes luxS, pfs, motA, and fliA were downregulated, leading to decreased csgD expression. At 1/2 MIC, csgD expression was significantly reduced. In conclusion, cefoxitin inhibits biofilm formation in multidrug-resistant E. coli by down-regulating key genes, offering a potential strategy to mitigate resistance and control infections in calves caused by biofilm-positive E. coli isolates.

头孢西丁抑制生物膜的形成参与抗微生物药耐多药大肠杆菌。
本研究探讨了小牛分离的大肠杆菌生物膜形成与抗生素耐药性之间的关系。采用生物化学和分子方法鉴定分离菌株,并通过改进的结晶紫染色法评估其生物膜形成能力。采用微量肉汤稀释法测定18种抗生素对菌株的最低抑菌浓度(mic)。采用激光扫描共聚焦显微镜(LSCM)分析头孢西丁对生物膜形成的影响。此外,采用qRT-PCR方法评估生物膜相关基因(luxS、motA、fliA、pfs和csgD)在不同头孢西丁浓度下的表达水平。结果表明,抗菌素耐药性(AMR)与生物膜形成能力显著相关。头孢西丁在1/2和1 MIC时有效地减少了多重耐药大肠杆菌分离株的生物膜形成,且浓度越高,抑制作用越强。qs相关基因luxS、pfs、motA、fliA下调,导致csgD表达降低。1/2 MIC时,csgD表达显著降低。综上所述,头孢西丁通过下调关键基因抑制多重耐药大肠杆菌的生物膜形成,为减轻耐药和控制生物膜阳性大肠杆菌分离株引起的犊牛感染提供了一种潜在的策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Animal Biotechnology
Animal Biotechnology 工程技术-奶制品与动物科学
CiteScore
2.90
自引率
5.40%
发文量
230
审稿时长
>12 weeks
期刊介绍: Biotechnology can be defined as any technique that uses living organisms (or parts of organisms like cells, genes, proteins) to make or modify products, to improve plants, animals or microorganisms for a specific use. Animal Biotechnology publishes research on the identification and manipulation of genes and their products, stressing applications in domesticated animals. The journal publishes full-length articles and short research communications, as well as comprehensive reviews. The journal also provides a forum for regulatory or scientific issues related to cell and molecular biology applied to animal biotechnology. Submissions on the following topics are particularly welcome: - Applied microbiology, immunogenetics and antibiotic resistance - Genome engineering and animal models - Comparative genomics - Gene editing and CRISPRs - Reproductive biotechnologies - Synthetic biology and design of new genomes
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