Jia Liu, Chicheng Ma, Yu Cheng, Minzhi Wang, Guoqing Zhao, Liwei Huang, Ruigao Song, Xi Wang, Hongxia Li
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引用次数: 0
Abstract
m6A methylation is the most common mRNA modification in mammals and plays a significant role in regulating various biological functions. Some studies have demonstrated that the methyltransferase METTL3 can promote adipogenesis. However, the regulatory mechanisms of METTL14 and WTAP, both methyltransferases, in adipogenesis remain unclear. This study investigated their effects on bovine preadipocyte differentiation using siRNA-mediated knockdown combined with transcriptomic analysis. Silencing METTL14 and WTAP significantly impaired lipid droplet formation and revealed distinct regulatory pathways: METTL14 knockdown affected genes like JAK2 and STAT3, while WTAP suppression down-regulated PPARγ/FABP4 signalling pathway components. These findings demonstrate that WTAP specifically modulates bovine adipocyte differentiation through the PPARγ/FABP4 pathway.
期刊介绍：
Biotechnology can be defined as any technique that uses living organisms (or parts of organisms like cells, genes, proteins) to make or modify products, to improve plants, animals or microorganisms for a specific use. Animal Biotechnology publishes research on the identification and manipulation of genes and their products, stressing applications in domesticated animals. The journal publishes full-length articles and short research communications, as well as comprehensive reviews. The journal also provides a forum for regulatory or scientific issues related to cell and molecular biology applied to animal biotechnology.
Submissions on the following topics are particularly welcome:
- Applied microbiology, immunogenetics and antibiotic resistance
- Genome engineering and animal models
- Comparative genomics
- Gene editing and CRISPRs
- Reproductive biotechnologies
- Synthetic biology and design of new genomes
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