三七茎叶通过调节都藏猪肠道菌群,降低抗生素耐药基因的丰度。

IF 1.7 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-03-17 DOI:10.1080/10495398.2025.2471785
Wenjie Cheng, Lanlan Yi, Taojie Xu, Yuxiao Xie, Junhong Zhu, Xuancheng Guan, Qiuyan Li, Ying Huang, Yanguang Zhao, Sumei Zhao
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引用次数: 0

摘要

为了研究在饲料中添加三七茎叶后都藏猪肠道菌群和抗生素耐药基因(ARGs)的分布特征,采用宏基因组测序方法探索了都藏猪肠道菌群特征,并通过qPCR检测了14个ARGs和2个整合酶基因。结果表明,添加三七茎叶可提高都藏猪盲肠中厚壁菌门、乳酸杆菌和Pediococcus的相对丰度。在猪盲肠内容物中共检测到10个ARGs和2个整合酶基因。三七茎叶的添加降低了杜藏猪盲肠中总ARGs、ermB、tetO和tetW的相对丰度。网络分析结果表明,多个属是ARGs的潜在宿主。三七茎叶的添加可能通过降低杆状杆菌和黄酮因子等属的相对丰度来降低ARGs的相对丰度,从而降低ARGs传播的风险。本研究为合理利用三七茎叶防治ARGs提供了理论依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The stems and leaves of Panax notoginseng reduce the abundance of antibiotic resistance genes by regulating intestinal microbiota in Duzang pigs.

In order to study the distribution characteristics of intestinal microbiota and antibiotic resistance genes (ARGs) in Duzang pigs after adding stems and leaves of Panax notoginseng to the feed, the characteristics of intestinal microbiota were explored by metagenomic sequencing, and 14 ARGs and 2 integrase genes were detected by qPCR. The results showed that the addition of stems and leaves of P. notoginseng increased the relative abundance of Firmicutes, Lactobacillus and Pediococcus in the cecum of Duzang pigs. A total of 10 ARGs and 2 integrase genes were detected in the cecal contents of pigs. The addition of stems and leaves of P. notoginseng reduced the relative abundance of total ARGs, ermB, tetO and tetW in the cecum of Duzang pigs. The results of network analysis showed that multiple genera were potential hosts of ARGs. The addition of stems and leaves of P. notoginseng may reduce the relative abundance of ARGs by reducing the relative abundance of genera such as Corynebacterium and Flavonifractor, thereby reducing the risk of ARGs spread. This study provides a theoretical basis for the rational use of stems and leaves of P. notoginseng to control ARGs.

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来源期刊
Animal Biotechnology
Animal Biotechnology 工程技术-奶制品与动物科学
CiteScore
2.90
自引率
5.40%
发文量
230
审稿时长
>12 weeks
期刊介绍: Biotechnology can be defined as any technique that uses living organisms (or parts of organisms like cells, genes, proteins) to make or modify products, to improve plants, animals or microorganisms for a specific use. Animal Biotechnology publishes research on the identification and manipulation of genes and their products, stressing applications in domesticated animals. The journal publishes full-length articles and short research communications, as well as comprehensive reviews. The journal also provides a forum for regulatory or scientific issues related to cell and molecular biology applied to animal biotechnology. Submissions on the following topics are particularly welcome: - Applied microbiology, immunogenetics and antibiotic resistance - Genome engineering and animal models - Comparative genomics - Gene editing and CRISPRs - Reproductive biotechnologies - Synthetic biology and design of new genomes
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