{"title":"INSL3和WNT2B基因多态性对新疆齐拉黑羊、哈萨克羊和多朗羊季节繁殖性状的影响","authors":"Jingdong Bi, Sulaiman Yiming, Jiaqi Li, Quanfeng Wang, Manjun Zhai, Shaoqi Cao, Mengting Zhu, Hua Yang","doi":"10.1080/10495398.2025.2498677","DOIUrl":null,"url":null,"abstract":"<p><p>The purpose of this study was to investigate the polymorphism and genetic correlation of <i>INSL3</i> and <i>WNT2B</i> genes with seasonal estrus and litter size in three different Xinjiang sheep breeds. The genetic diversity of <i>INSL3</i> and <i>WNT2B</i> genes were analyzed, and their association with litter size and estrous traits were analyzed. The results showed that two SNPs (SNP1, SNP2) were detected in <i>INSL3</i> gene and there were three genotypes in SNP2 (<i>INSL3</i> (A100T)), named of AA, AT and TT, A was the dominant allele. Additionally, five SNPs (SNP3, SNP4, SNP5, SNP6, SNP7) were detected in the <i>WNT2B</i> gene and there were three genotypes in SNP4 (<i>WNT2B</i> (G126T)), named GG, GT and TT, G was dominant allele. SNP2 was in Hardy-Weinberg equilibrium in three sheep breeds (<i>P</i> > 0.05). SNP4 was deviated from Hardy-Weinberg equilibrium in three sheep breeds (<i>P</i> < 0.05). Further, AT genotype of SNP2 (<i>INSL3</i> (A100T)) could significantly affect the estrus trait in Duolang sheep and Qira black sheep, and related to the litter size in Duolang sheep. The <i>WNT2B</i> significantly affected the estrus and litter size of Duolang sheep and Qira black sheep. <i>INSL3</i> (A100T) and <i>WNT2B</i> (G126T) may be potential molecular markers for controlling seasonal reproductive trait in sheep.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2498677"},"PeriodicalIF":1.7000,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effects of <i>INSL3</i> and <i>WNT2B</i> gene polymorphisms on seasonal reproductive traits of Xinjiang Qira black sheep, Kazakh sheep and Duolang sheep.\",\"authors\":\"Jingdong Bi, Sulaiman Yiming, Jiaqi Li, Quanfeng Wang, Manjun Zhai, Shaoqi Cao, Mengting Zhu, Hua Yang\",\"doi\":\"10.1080/10495398.2025.2498677\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The purpose of this study was to investigate the polymorphism and genetic correlation of <i>INSL3</i> and <i>WNT2B</i> genes with seasonal estrus and litter size in three different Xinjiang sheep breeds. The genetic diversity of <i>INSL3</i> and <i>WNT2B</i> genes were analyzed, and their association with litter size and estrous traits were analyzed. The results showed that two SNPs (SNP1, SNP2) were detected in <i>INSL3</i> gene and there were three genotypes in SNP2 (<i>INSL3</i> (A100T)), named of AA, AT and TT, A was the dominant allele. Additionally, five SNPs (SNP3, SNP4, SNP5, SNP6, SNP7) were detected in the <i>WNT2B</i> gene and there were three genotypes in SNP4 (<i>WNT2B</i> (G126T)), named GG, GT and TT, G was dominant allele. SNP2 was in Hardy-Weinberg equilibrium in three sheep breeds (<i>P</i> > 0.05). SNP4 was deviated from Hardy-Weinberg equilibrium in three sheep breeds (<i>P</i> < 0.05). Further, AT genotype of SNP2 (<i>INSL3</i> (A100T)) could significantly affect the estrus trait in Duolang sheep and Qira black sheep, and related to the litter size in Duolang sheep. The <i>WNT2B</i> significantly affected the estrus and litter size of Duolang sheep and Qira black sheep. <i>INSL3</i> (A100T) and <i>WNT2B</i> (G126T) may be potential molecular markers for controlling seasonal reproductive trait in sheep.</p>\",\"PeriodicalId\":7836,\"journal\":{\"name\":\"Animal Biotechnology\",\"volume\":\"36 1\",\"pages\":\"2498677\"},\"PeriodicalIF\":1.7000,\"publicationDate\":\"2025-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Animal Biotechnology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1080/10495398.2025.2498677\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/5/3 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"AGRICULTURE, DAIRY & ANIMAL SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Animal Biotechnology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1080/10495398.2025.2498677","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/5/3 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
Effects of INSL3 and WNT2B gene polymorphisms on seasonal reproductive traits of Xinjiang Qira black sheep, Kazakh sheep and Duolang sheep.
The purpose of this study was to investigate the polymorphism and genetic correlation of INSL3 and WNT2B genes with seasonal estrus and litter size in three different Xinjiang sheep breeds. The genetic diversity of INSL3 and WNT2B genes were analyzed, and their association with litter size and estrous traits were analyzed. The results showed that two SNPs (SNP1, SNP2) were detected in INSL3 gene and there were three genotypes in SNP2 (INSL3 (A100T)), named of AA, AT and TT, A was the dominant allele. Additionally, five SNPs (SNP3, SNP4, SNP5, SNP6, SNP7) were detected in the WNT2B gene and there were three genotypes in SNP4 (WNT2B (G126T)), named GG, GT and TT, G was dominant allele. SNP2 was in Hardy-Weinberg equilibrium in three sheep breeds (P > 0.05). SNP4 was deviated from Hardy-Weinberg equilibrium in three sheep breeds (P < 0.05). Further, AT genotype of SNP2 (INSL3 (A100T)) could significantly affect the estrus trait in Duolang sheep and Qira black sheep, and related to the litter size in Duolang sheep. The WNT2B significantly affected the estrus and litter size of Duolang sheep and Qira black sheep. INSL3 (A100T) and WNT2B (G126T) may be potential molecular markers for controlling seasonal reproductive trait in sheep.
期刊介绍：
Biotechnology can be defined as any technique that uses living organisms (or parts of organisms like cells, genes, proteins) to make or modify products, to improve plants, animals or microorganisms for a specific use. Animal Biotechnology publishes research on the identification and manipulation of genes and their products, stressing applications in domesticated animals. The journal publishes full-length articles and short research communications, as well as comprehensive reviews. The journal also provides a forum for regulatory or scientific issues related to cell and molecular biology applied to animal biotechnology.
Submissions on the following topics are particularly welcome:
- Applied microbiology, immunogenetics and antibiotic resistance
- Genome engineering and animal models
- Comparative genomics
- Gene editing and CRISPRs
- Reproductive biotechnologies
- Synthetic biology and design of new genomes
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