Applied Soil Ecology最新文献

{"title":"Synergistic effects of biochar and organic fertilizer on root exudates, microbial community, and yield in dry direct-seeded rice","authors":"Xiaoqiang Cao ,&nbsp;Deping Liu ,&nbsp;Jilong Liu ,&nbsp;Lingling Zhang ,&nbsp;Mo Li ,&nbsp;Guoxin Shi ,&nbsp;Hui Liu ,&nbsp;Hao Wang ,&nbsp;Weili Sun","doi":"10.1016/j.apsoil.2025.106501","DOIUrl":"10.1016/j.apsoil.2025.106501","url":null,"abstract":"<div><div>Dry direct-seeded rice (DDSR) offers significant water-saving potential but often suffers from unstable yields, low nutrient-use efficiency, and disrupted root-soil-microbe interactions under alternating wet-dry cycles. Although biochar and organic fertilizers have been widely reported to improve soil quality and microbial function, their synergistic effects on DDSR root exudates, microorganisms, and crops require further investigation. Therefore, we conducted a field experiment with six treatments integrating biochar application (0, 5, and 10 t/hm²) and organic fertilizer substitution (0 % or 20 % fertilizer N) to investigate their synergistic regulation of root exudates, microbial communities, and yield. The findings revealed that the root exudative capacity of DDSR was enhanced (metabolites of B1N2 up 17, down 9 and B2N2 up 9, down 6) by the synergistic effect of biochar and organic fertilizer. Random forest modeling identified lipids and lipid-like molecules and organic acids and derivatives as the most critical metabolome between treatments under biochar and organic fertilizer application. Over-Representation Analysis (ORA) revealed that arginine and proline, glycine, serine, threonine, and glycerophospholipid were the key regulatory pathways of biochar and organic fertilizer. Secondly, stochastic processes dominated the assembly process of soil microorganisms, and root exudates and soil physicochemical properties under biochar and organic fertilizers combined to promote mutual shifts in soil microbial community assembly drivers, which altered soil microbial community diversity. Notably, the microbial community-root exudates-soil physicochemical property interactions under the effect of biochar and organic fertilizers were dominated by synergistic effects and supplemented by antagonistic effects, which together promoted the root development of dry direct-seeded rice and significantly increased rice yield (3.96–11.64 %). In conclusion, the results of the study confirmed the feasibility of stable and high rice yield and microecological regulation in dry direct-seeded paddy fields by regulating key root exudates and microbial diversity.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"216 ","pages":"Article 106501"},"PeriodicalIF":5.0,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145227712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soil clay content determined the temperature response of N2O emissions derived from denitrification","authors":"Lixin Jia ,&nbsp;Hanhan Yang ,&nbsp;Yong Li ,&nbsp;Zhangliu Du ,&nbsp;Xiaotang Ju ,&nbsp;Yue Li ,&nbsp;Di Wu","doi":"10.1016/j.apsoil.2025.106500","DOIUrl":"10.1016/j.apsoil.2025.106500","url":null,"abstract":"<div><div>Soil nitrous oxide (N₂O) emissions resulting from denitrification constitute a significant source of greenhouse gas emissions in the agricultural sector. However, how warming affects denitrification-derived N₂O emissions in croplands remains poorly understood, presenting challenges for predicting greenhouse gas feedback loops under climate change. In this study, we evaluated the temperature sensitivity of potential N₂O emissions from denitrification in cropland soils, employing the N₂O site preference approach to differentiate between bacterial and fungal contributions. Our findings reveal that fungal denitrification dominated N₂O emissions; however, warming decreased its contribution from 57.5 % to 51.5 %. Denitrification-derived N₂O emissions displayed significant variability across sites, and a strong positive correlation was observed between soil clay content and the Q₁₀ value of N₂O. This relationship was further supported by the global data provided. Our findings underscore the importance of incorporating both soil properties and fungal processes when projecting future N₂O emissions under global warming scenarios.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"216 ","pages":"Article 106500"},"PeriodicalIF":5.0,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145227714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polyethylene microplastics modulate zinc oxide nanoparticle toxicity: Effects on nutrient dynamics and rhizosphere bacterial community in pakchoi","authors":"Kun Zhang ,&nbsp;Junrui Bai ,&nbsp;Jiayi Wang ,&nbsp;Jie Guo ,&nbsp;Hongda Sun ,&nbsp;Jinshan Liu ,&nbsp;Kadambot H.M. Siddique ,&nbsp;Hui Mao","doi":"10.1016/j.apsoil.2025.106505","DOIUrl":"10.1016/j.apsoil.2025.106505","url":null,"abstract":"<div><div>The coexistence of zinc oxide nanoparticles (ZnO NPs) and polyethylene microplastics (PE-MPs), driven by industrial and agricultural activities, raises concerns about their combined impacts on the environment. However, the mechanisms by which PE-MPs influence the biological effects of ZnO NPs remain poorly understood. This study explored the effects of varying doses of PE-MPs and ZnO NPs simultaneously and ZnO NPs alone on pakchoi (<em>Brassica rapa</em> subsp. <em>chinensis</em>), focusing on nutrient uptake, elemental distribution, soil bacterial communities, and environmental risks. Exposure to 900 mg kg⁻¹ ZnO NPs alone significantly reduced pakchoi shoot and root dry weights by 50.3 % and 62.6 %, respectively, while increasing Zn accumulation in both tissues. Conversely, co-exposure with PE-MPs mitigated the phytotoxic effects of ZnO NPs, decreasing Zn uptake in pakchoi. Additionally, ZnO NPs alone significantly reduced soil bacterial α-diversity, with ACE and Chao1 indices decreasing by 34.5 % and 37.5 %, respectively, compared to the control. However, the presence of PE-MPs reversed these adverse effects on microbial diversity. This research uncovers how PE-MPs regulate ZnO NPs biological toxicity, establishing a scientific groundwork for assessing the co-exposure's toxicological mechanisms and environmental risks.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"216 ","pages":"Article 106505"},"PeriodicalIF":5.0,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145227713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contrasting responses of surface and subsurface soil microbiome to ecological restoration in two types of steppe because of different changes in plant and soil properties","authors":"Xiaojiang Yang ,&nbsp;Xiangjun Yun ,&nbsp;Wenbo Zhang ,&nbsp;Paul C. Struik ,&nbsp;Shenyi Jiang ,&nbsp;Xiangjian Tu ,&nbsp;Ke Jin ,&nbsp;Zhen Wang","doi":"10.1016/j.apsoil.2025.106493","DOIUrl":"10.1016/j.apsoil.2025.106493","url":null,"abstract":"<div><div>Grazing exclusion contributes to the restoration of degraded grassland, including its soil. Whether restoration merely affects the surface layer or also penetrates into the subsurface layer remains unclear. Therefore, taking the typical steppe and desert steppe in the Mongolian Plateau as cases, this study investigated the effects of grazing exclusion on soil microbial diversity, composition, and function in the soil surface (0–10 cm) and subsurface (10–30 cm). Bacterial diversity was higher in the surface layer than in the subsurface layer in the typical steppe, but the opposite was true in the desert steppe. Grazing exclusion significantly increased fungal diversity in the surface layer (typical steppe) or in both layers (desert steppe). Grazing exclusion significantly altered bacterial (Actinobacteria, Proteobacteria) and fungal (Basidiomycota, Ascomycota) community composition in both steppe types. In the typical steppe, both bacterial and fungal communities differed between soil layers, whereas only bacterial communities varied between soil layers in the desert steppe. Co-occurrence network stability correlated with bacterial (not fungal) community composition and was linked to soil nutrients (e.g., dissolved organic carbon, soil organic carbon, and total nitrogen) across steppe types and soil layers. Overall, our research showed that grazing exclusion showed higher proportion and stronger functioning of soil microbiome associated with plant growth promotion, nutrient acquisition, or pathogen suppression in the typical and desert steppes. The difference in microbiome between the surface layer and subsurface layer of the typical steppe depended on bacteria and fungi, while that difference only depended on bacteria for the desert steppe.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"215 ","pages":"Article 106493"},"PeriodicalIF":5.0,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impacts of wheat straw and sulfate application on soil cadmium availability and bacterial community composition in two waterlogged soils with contrasting cadmium levels","authors":"Xianlin Ke ,&nbsp;Yifei Li ,&nbsp;Mingming Tao ,&nbsp;Yanhua Qiu ,&nbsp;Songyan Li ,&nbsp;Xiaosong Yang ,&nbsp;Zhengyi Hu","doi":"10.1016/j.apsoil.2025.106496","DOIUrl":"10.1016/j.apsoil.2025.106496","url":null,"abstract":"<div><div>Straw incorporation and sulfur fertilizer are considered promising agronomic practices for remediating cadmium (Cd)-contaminated soil. However, their combined effects on Cd availability and soil bacterial communities in paddy soil remain unclear and may vary depending upon initial soil Cd levels. In this study, we conducted a 162-day incubation experiment under waterlogged conditions, applying wheat straw (0 and 1 % <em>w</em>/w) and sulfate (0 and 30 mg kg⁻¹) individually or in combination to soils with high Cd (3.18 mg Cd kg⁻¹) and low Cd (0.18 mg Cd kg⁻¹) contamination. At the early stage, straw alone (W) or in combination with sulfate (W + S) reduced dissolved Cd concentration in both the low- and high-Cd soils. However, an increase in exchangeable Cd was observed only in the low-Cd soil during this period. Sulfate application (S) alone had no significant effect on either dissolved or exchangeable Cd. Both W and W + S treatments reduced bacterial community alpha diversity in the low- and high-Cd soil, primarily effecting the relative abundance of Firmicutes, Bacteroidota, Proteobacteria, and Acidobacteriota. Change in bacterial communities and soil properties, including an increase in dissolved organic carbon and decreases in Eh and pH, were associated with shifts in Cd availability. The effect of straw and sulfate application on Cd availability and bacterial community diversity varied with Cd contamination levels. Overall, straw incorporation, either alone or with sulfate, may help reduce Cd availability and modulate microbial communities in high-Cd paddy soil.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"215 ","pages":"Article 106496"},"PeriodicalIF":5.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Weathering modulates soil microbial biodiversity in a Sygera Mountain alpine forest, Tibetan Plateau","authors":"Hongzhe Jiao ,&nbsp;Qiuyu Chen ,&nbsp;Bin Niu ,&nbsp;Xiaoqin Yang ,&nbsp;Guiyao Zhou ,&nbsp;Ang Hu ,&nbsp;Jian Wang ,&nbsp;Guicai Si ,&nbsp;Jiangrong Li ,&nbsp;Eryuan Liang ,&nbsp;Manuel Delgado-Baquerizo ,&nbsp;Gengxin Zhang","doi":"10.1016/j.apsoil.2025.106495","DOIUrl":"10.1016/j.apsoil.2025.106495","url":null,"abstract":"<div><div>Alpine ecosystems are highly vulnerable to global change. In alpine ecosystems, weathering (as represented by weathering indices reflecting the integrated product of chemical, physical, and biological processes) associated with biotic activity, is a critical factor explaining soil environmental conditions. However, the contribution of weathering to explaining soil biodiversity in alpine ecosystems remains poorly quantified. Here, we assessed the contribution of weathering to explain prokaryotic and eukaryotic diversities across an elevational gradient in an alpine forest. Notably, along an elevational gradient, we found significant negative relationships between weathering and climatic variables, challenging the conventional unidirectional paradigm of climate-dominated weathering processes. The inverted weathering index of Parker negatively correlated with prokaryotic diversity (<em>P</em> = 0.006, R² = 0.30). Variation partitioning analysis revealed that weathering explained 17 % of the variance in prokaryotic diversity, 36 % in eukaryotic diversity and 13 % in multidiversity. Structural equation modeling further indicated that weathering had significant standardized direct effects on prokaryotic (<em>R</em> = 0.36) and eukaryotic diversities (<em>R</em> = 0.49), respectively. Our study demonstrates the critical role of weathering in shaping prokaryotic and eukaryotic diversities, particularly through regulatory mechanisms independent of climatic variables, emphasizing its importance for understanding and conserving soil biodiversity in alpine ecosystems facing global change.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"215 ","pages":"Article 106495"},"PeriodicalIF":5.0,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering the genome of the endophytic bacterium Pseudomonas retamae type strain RB5T: A prominent plant growth promoter for wheat with potential applications in sustainable agriculture","authors":"Nawel Selami ,&nbsp;Fatima El-Haouaria Zitouni-Haouar ,&nbsp;Chahira Zerouki ,&nbsp;Katia Khadidja Abdeddaim ,&nbsp;Chahrazed Aibeche ,&nbsp;Nassima Draou ,&nbsp;Omar Khelil ,&nbsp;Slimane Choubane ,&nbsp;Makaoui Maatallah ,&nbsp;Hassiba Bokhari ,&nbsp;Ikram Madani ,&nbsp;Khadidja Ouenzar ,&nbsp;Assia Zemmour ,&nbsp;Mohamed Kerkoud ,&nbsp;Amine Drici ,&nbsp;Bacem Mnasri ,&nbsp;Abderrezak Djabeur","doi":"10.1016/j.apsoil.2025.106491","DOIUrl":"10.1016/j.apsoil.2025.106491","url":null,"abstract":"<div><div>This study focused on unraveling the plant growth-promoting properties of <em>Pseudomonas retamae</em> type strain RB5T, a newly described species isolated from root nodules of <em>Retama monosperma</em>, a drought-tolerant coastal legume, and evaluating its effects on wheat growth and resilience<em>. P. retamae</em> RB5T exhibited several PGP traits, including the production of indole acetic acid (IAA, 18 ± 3.48 μg/mL) and phosphate solubilization activity (8.14 ± 0.25 cm). It also tolerated salinity up to 6 %, thrived at 42 °C, and displayed antagonistic activity against <em>Fusarium oxysporum</em>. Notably, the strain significantly enhanced shoot and root biomass of wheat seedlings in vitro, under greenhouse conditions, and in field trials. To gain insight into the genetic basis of these traits and to better understand the endophytic nature of the strain, we conducted in silico analysis of the complete genome. Genome annotation revealed gene clusters related to auxin biosynthesis (<em>iaaH</em>, <em>iaaM</em>), nitrogen metabolism (<em>glnGKL</em>, <em>cyn</em>, <em>nas</em>, <em>nor</em>), phosphate solubilization (<em>phnCDEP</em>, <em>phoABHLU</em>, <em>pstABCHS</em>), and antimicrobial compound production (<em>phzF</em>, <em>fusABCDE</em>). Genes involved in abiotic stress tolerance, such as <em>katE</em>, superoxide dismutases, <em>hsp</em>, <em>capA</em>, <em>betT</em>, and heavy metal resistance, were also identified. These findings confirm the potential of <em>P. retamae</em> RB5T as an eco-friendly bioresource primed for fostering plant growth, particularly in drought-prone regions.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"215 ","pages":"Article 106491"},"PeriodicalIF":5.0,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Do neighbours matter? Soil mycobiome and mycorrhizal colonization of Ulmus laevis seedlings grown in the vicinity of arbuscular or ectomycorrhizal trees","authors":"M.B. Kujawska,&nbsp;R. Wilgan,&nbsp;M. Rudawska,&nbsp;T. Leski","doi":"10.1016/j.apsoil.2025.106476","DOIUrl":"10.1016/j.apsoil.2025.106476","url":null,"abstract":"<div><div>The influence of arbuscular (AM) or ectomycorrhizal (ECM) trees and a mixture of tree mycorrhizal types on the soil mycobiome is not yet well understood. To address this knowledge gap, a pot experiment was designed in which AM <em>Ulmus laevis</em> seedlings were grown in the presence of AM (<em>Acer campestre</em>) and ECM (<em>Quercus robur</em>, <em>Carpinus betulus</em>) trees. A possible ECM symbiosis of <em>U. laevis</em>, a topic that is often misinterpreted in <em>Ulmus</em> research, has also been analysed. Soil mycobiomes were identified by Illumina sequencing of ITS2 rDNA, ectomycorrhizas by Sanger sequencing ITS rDNA, and AM colonization through microscopic observations of AM structures in <em>Ulmus</em> roots.</div><div>The soil mycobiome differed between variants with AM trees only and those with admixture of ECM trees. These differences were observed at the taxonomic level, fungal trophic guilds, and in the contribution of ECM fungi. The structures resembling ECM on <em>Ulmus</em> roots were formed by non-ectomycorrhizal fungi. Microscopic analysis of <em>Ulmus</em> roots revealed, that <em>Ulmus</em> seedlings form only AM symbioses, regardless of the ECM trees in their vicinity. Further research on mature <em>Ulmus</em> trees in their natural habitats is needed to clarify their potential to enter into ECM symbiosis. The pot experiment results emphasise the pressing necessity for additional research into AM and ECM tree interactions in natural environments, as this is pivotal to comprehending changes in soil mycobiomes and their repercussions on temperate forest ecosystems.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"215 ","pages":"Article 106476"},"PeriodicalIF":5.0,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochar modulates microbial- and plant-derived carbon allocation in soil aggregates to enhance organic carbon sequestration in paddy fields under different water management practices","authors":"Yu Han ,&nbsp;Peng Chen ,&nbsp;Zhongxue Zhang ,&nbsp;Xiaoyuan Yan ,&nbsp;Guangbin Zhang ,&nbsp;Zhijuan Qi ,&nbsp;Zuohe Zhang ,&nbsp;Sicheng Du ,&nbsp;Li Xue","doi":"10.1016/j.apsoil.2025.106497","DOIUrl":"10.1016/j.apsoil.2025.106497","url":null,"abstract":"<div><div>Microbial- and plant-derived carbon (C) constitutes a substantial portion of soil organic carbon (SOC) pools. However, their dynamics under biochar amendment in soil aggregates and roles in SOC sequestration remain unclear, especially across different water management practices. To address these knowledge gaps, we examined the bulk SOC content and the proportions of soil aggregate fractions (large macroaggregates (LM), macroaggregates (MA), microaggregates (MI), and silt + clay (SC)) in paddy fields under four treatments: flooded irrigation without biochar amendment (FI), flooded irrigation with biochar amendment (FIB), controlled irrigation without biochar amendment (CI), and controlled irrigation with biochar amendment (CIB). The distributions of microbial- and plant-derived C in soil aggregate fractions were measured through the analysis of amino sugars and lignin phenols, along with their contributions to SOC. Biochar amendment increased bulk SOC by 17.06 % under CIB and 18.10 % under FIB compared to non-biochar treatments, with divergent mechanisms operating across water management practices. Biochar amendment enhanced plant-derived C contents in the LM, MA, and MI fractions regardless of water management practice, in addition to increased microbial-derived C content in the MI and SC fractions of the CI treatment. However, no significant amendment effect was observed on microbial-derived C contents in the FI treatment. Biochar amendment increased the contribution of plant-derived C to SOC in the MA fraction under different water management practices, while it enhanced the contribution of microbial-derived C to SOC in the MI fraction only under CI. Additionally, linear regression analysis revealed that SOC sequestration was strongly linked to microbial-derived C in MI fractions and plant-derived C in MA fractions. These findings demonstrate that biochar amendment promotes SOC sequestration through water management-dependent mechanisms: preferential accumulation of plant-derived C in MA fractions under both FI and CI, alongside enhanced microbial-derived C in MI fractions specifically under CI. This study provides novel insights into the mechanisms by which biochar amendment enhances SOC sequestration in paddy soils.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"215 ","pages":"Article 106497"},"PeriodicalIF":5.0,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soil bacterial diversity drives shifts in dominant plants' fine root C:N:P stoichiometry across an altitudinal gradient","authors":"Yulong Ma ,&nbsp;Wangsuo Liu ,&nbsp;Kaiyang Qiu ,&nbsp;Yi Yang ,&nbsp;Guohui Wang ,&nbsp;Xiang Zheng ,&nbsp;Xuying Hai ,&nbsp;Bin Xue ,&nbsp;Hongmei Wang ,&nbsp;Jianping Li ,&nbsp;Panxing He ,&nbsp;Yingzhong Xie","doi":"10.1016/j.apsoil.2025.106486","DOIUrl":"10.1016/j.apsoil.2025.106486","url":null,"abstract":"<div><div>Fine roots (diameter ≤ 2 mm) play a crucial role in regulating the biogeochemical cycles of mountain ecosystems, yet how their stoichiometric characteristics are influenced by rhizosphere microorganisms and their functional genes remains poorly understood. This study examined six vegetation zones spanning an altitude range of 1350–2950 m in the Helan Mountains, China, focusing on the rhizosphere of dominant plants. Specifically, it investigates fine root carbon (C), nitrogen (N), and phosphorus (P) contents, their stoichiometric ratios, microbial diversity, and the enrichment of functional genes linked to C, N, and P cycling processes. For each plant life form (arbor, shrub, and herb), sampling was carried out along the altitudinal gradient, targeting four specific elevations where each life form dominates. Furthermore, the research evaluated the key factors that influence fine root stoichiometric ratios. We found that as altitude increases, arbor species trigger a cascade effect through coordinated reductions in soil nutrient (soil organic carbon, total nitrogen, total phosphorus), and microbial diversity, which drives adaptive differentiation in fine root stoichiometry. Meanwhile, under the same lifeform, the primary factor influencing fine root stoichiometry for different plants at different altitudes is plant species. In the microbial driving mechanism, bacterial diversity emerged as the primary determinant of fine root stoichiometry in dominant plants. Additionally, the enrichment of microbial C, N, and P cycling functional genes had no significant impact on fine root stoichiometry. Our findings provide insights for ecological restoration and conservation to enhance the stability and sustainability of mountain ecosystems.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"215 ","pages":"Article 106486"},"PeriodicalIF":5.0,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145155209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}