Applied Soil Ecology最新文献

{"title":"Organic fertilizations alter the abundance and diversity of soil microbial genes involved in C, N, P mineralization in a coastal poplar plantation","authors":"Tingting Ren ,&nbsp;Jiahui Liao ,&nbsp;Xiaoming Zou ,&nbsp;Yuanyuan Li ,&nbsp;Juanping Ni ,&nbsp;Ke Shi ,&nbsp;Long Jin ,&nbsp;Manuel Delgado-Baquerizo ,&nbsp;Honghua Ruan","doi":"10.1016/j.apsoil.2025.106001","DOIUrl":"10.1016/j.apsoil.2025.106001","url":null,"abstract":"<div><div>Organic fertilization supports the sustainability of managed ecosystems; however, investigations into how microbial-driven mineralization processes of carbon (C), nitrogen (N), and phosphorus (P) interact with soil functions under the application of organic fertilizers remain limited. We investigated the impacts of six years of applying biogas-slurry and biochar on the abundance and diversity of soil microbial genes involved in C, N, and P mineralization in a poplar plantation. Our findings indicated that the addition of biogas-slurry alone, as well as in combination with biochar, reduced the diversity of microbial genes involved in C, N, and P mineralization. The addition of biogas-slurry increased the abundance of these genes, but the application of biochar decreased it. Using thresholds from the eco-enzyme vector model, we found that the addition of biogas-slurry, either alone or in combination with biochar, alleviated microbial P limitation by decreasing the N:P ratio of microbial biomass and increasing soil dissolved organic C (DOC). Additionally, linear regression indicated that the alleviation of microbial P limitation suppressed the diversity of genes and promoted the abundance of genes involved in C mineralization. Random forest and partial dependence analyses showed that increased DOC was the major factor responsible for the decreased diversity of microbial genes and increased abundance of genes involved in N mineralization. The SOC: TN ratio was negatively correlated with the abundance of genes involved in N and P mineralization. These findings highlight the inconsistent responses of the abundance and diversity of microbial genes involved in C, N, and P mineralization to the application of organic fertilizers in managed ecosystems. Additionally, these varying responses are regulated by increasing nutrient supplies and alleviating microbial P limitation. Our findings provide a new understanding of soil carbon and nutrient cycling and suggest the application of organic fertilizers to facilitate the sustainable management of ecosystems in the future.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"208 ","pages":"Article 106001"},"PeriodicalIF":4.8,"publicationDate":"2025-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143526940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rhizosphere soil microbial communities and nitrogen transformation response to forest fire smoke","authors":"Ziyan Huang ,&nbsp;Xiaoyu Zhan ,&nbsp;Mulualem Tigabu ,&nbsp;Yan He ,&nbsp;Zhehan Li ,&nbsp;Guangyu Wang ,&nbsp;Futao Guo","doi":"10.1016/j.apsoil.2025.105990","DOIUrl":"10.1016/j.apsoil.2025.105990","url":null,"abstract":"<div><div>Nitrogen (N) is a limiting element in terrestrial ecosystems, and soil microorganisms play a crucial role in N nutrient cycling. Forest fires, as significant drivers of global change, release large amounts of smoke pollutants that deposit nitrogen-containing compounds, such as nitrate (NO₃-N) and ammonium (NH₄-N), into the soil. These compounds enhance the availability of bioavailable N, influencing the geochemical cycling of N in forest ecosystems. However, our understanding of how forest fire smoke deposition alters soil microorganisms and influences soil N transformation processes remains limited. To address this, we employed metagenomic techniques to analyze differences in microbial communities and N transformation functional genes in the rhizosphere soil of <em>Cunninghamia lanceolata</em> (Lamb.) Hook under varying concentrations of smoke deposition. Our results indicated that, low-concentration smoke deposition significantly (<em>P</em> &lt; 0.05) increased N concentration and N transformation enzyme activity in the rhizosphere soil compared to the control group. After 7 days of low concentration smoke deposition, the net ammonification rate and net nitrification rate were 2.51 and 3.02 times higher, respectively, than in the control. The abundance of functional genes related to soil N loss mediated by microorganisms, such as those involved in nitrification and denitrification processes, increased while functional genes associated with N fixation and transport exhibited less pronounced positive effects. This suggests that N input from forest fires may not persist in soil over time, as evidenced by decreased soil N concentration. Furthermore, Partial Least Squares-Path Modelling analysis demonstrated that soil N conversion enzyme activity had a significant positive effect on N functional microorganisms under low-concentration forest fire smoke deposition. Overall, these findings highlight that smoke deposition affects soil N transformation by altering soil enzyme activity, N content, and microbial communities, and lower smoke concentration appears to have a more beneficial impact on soil N transformation processes.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"208 ","pages":"Article 105990"},"PeriodicalIF":4.8,"publicationDate":"2025-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143526941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Environmental and microhabitat influences on microbiota of snow-active Collembola in sub-zero temperatures","authors":"Cao Hao ,&nbsp;Yunga Wu ,&nbsp;Ting-Wen Chen ,&nbsp;Nadieh de Jonge ,&nbsp;Guoliang Xu ,&nbsp;Donghui Wu ,&nbsp;Jeppe Lund Nielsen","doi":"10.1016/j.apsoil.2025.105998","DOIUrl":"10.1016/j.apsoil.2025.105998","url":null,"abstract":"<div><div>Animal-associated microbiotas provide essential services to their host and they have been extensively studied during the snow free periods; however, the microbiotas of soil animals in harsh environments, particularly Collembola, a key group of soil microarthropods tolerating sub-zero temperatures and remaining active in snow-covered regions, are not well understood yet. In this study, we investigated the gut microbiota of the Collembola species <em>Desoria ruseki</em>, which is widely distributed in Northeast China, using 16S rRNA gene amplicon sequencing from different sites. Our findings revealed that the potential symbiotic microbiota and food microbiota of Collembola did not exhibit a distance-decay pattern, in contrast to the microbial communities found in snow and litter. The microbial communities associated with snow-active Collembola populations differed significantly among sites with snow being more influential than litter in shaping microbial communities. Total carbon, total nitrogen, ammonium nitrogen and nitrate nitrogen in snow were key factors influencing the food microbiota of Collembola, while total carbon in litter significantly affected their potential symbiotic microbiota. Collembola-associated bacteria, such as <em>Lautropia</em>, <em>Streptomyces</em>, <em>Mycobacterium</em>, <em>Marmoricola</em>, and <em>Fridmanniella</em> responded to changes in physicochemical properties. Our results suggest that different microbial groups associated with snow-active Collembola show distinct driving patterns by microhabitat conditions. The findings of this study can improve our understanding of the gut microbiota assembly of soil arthropods active in snowy regions.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"208 ","pages":"Article 105998"},"PeriodicalIF":4.8,"publicationDate":"2025-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143526993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green manuring increases network complexity and deterministic assembly of diazotrophic community in a subtropical paddy soil","authors":"Xianchu Su ,&nbsp;Luyuan Sun ,&nbsp;Yuntao Kang ,&nbsp;Mengmeng Feng ,&nbsp;Junkang Zhu ,&nbsp;Anqi Wang ,&nbsp;Zi-Yang He ,&nbsp;Shengsheng Jin ,&nbsp;Jia Liu ,&nbsp;Ji-Zheng He ,&nbsp;Yongxin Lin","doi":"10.1016/j.apsoil.2025.105997","DOIUrl":"10.1016/j.apsoil.2025.105997","url":null,"abstract":"<div><div>Chinese milk vetch (<em>Astragalus sinicus</em> L.) is widely used as green manure, fixing dinitrogen (N₂) during its growing season to enhance soil fertility and crop yields when incorporated into soils. Straw return is another common farmland management practice in paddy fields. However, how leguminous green manure and straw return influence free-living diazotrophic bacteria during the subsequent rice-growing season is not well understood. To address this, we conducted an eight-year rice-rice-green manure rotation and straw return experiment in southern China to assess the effects of green manuring and straw return on diazotrophic communities at various rice growing stages. The results showed that straw return increased diazotrophic abundance, whereas green manuring did not significantly affect it. Neither green manuring nor straw return influenced diazotrophic diversity, though both significantly altered diazotrophic community structure. Dissolved organic carbon, total nitrogen, nitrate and ammonium were identified as key factors shaping community structure. Green manuring decreased the relative abundance of <em>Desulfomonilia</em> by 31.1 % whereas straw return increased it by 18.7 %. Compared to the control, green manuring rather than straw return enhanced the complexity of diazotrophic co-occurrence network. While stochastic processes dominated diazotrophic community assembly in the rice paddy soils, green manuring increased the relative importance of deterministic process. Taken together, these findings suggest that green manuring may be a more effective management practice for shaping diazotrophic community dynamics in paddy soils. The influence of green manuring on deterministic processes and network complexity underscores its potential to enhance the stability and functionality of diazotrophic communities, potentially contributing to long-term soil health and agricultural sustainability.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"208 ","pages":"Article 105997"},"PeriodicalIF":4.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143519327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soil pH determines the shift of key microbial energy metabolic pathways associated with soil nutrient cycle","authors":"Akari Mitsuta ,&nbsp;Késia Silva Lourenço ,&nbsp;Bruna Gonçalves de Oliveira ,&nbsp;Ohana Yonara de Assis Costa ,&nbsp;Heitor Cantarella ,&nbsp;Eiko Eurya Kuramae","doi":"10.1016/j.apsoil.2025.105992","DOIUrl":"10.1016/j.apsoil.2025.105992","url":null,"abstract":"<div><div>Soil pH is one of the most important factors influencing microbial activity and function. In agricultural land, soil acidification results in decline of microbial abundance and diversity, and is associated with increased N₂O emissions. Moreover, soil pH is a crucial factor determining the effect of fertilizer on soil microbial abundance and functionality. However, the interaction effect of soil pH and the application of fertilizer on the abundance of key metabolic pathways involved in nitrogen, carbon, and sulfur cycles remains unclear. Therefore, we modified the original pH of soil (pH 6.0) to pH 4.5 by adding elemental sulfur to the soil and compared the effects of chemical (urea), organic fertilizer (concentrated vinasse), and the combination of both (urea and concentrated vinasse) on soil microbial functions under low soil pH (pH 4.5) and high soil pH (pH 6.0) conditions. Shotgun metagenome sequencing was conducted to obtain microbial functional gene abundance, and the data were analyzed using a model-based statistical approach to determine potential interactions among energy metabolic pathways (i.e., nitrogen, sulfur, methane metabolisms, and carbon fixation). Our result showed the strong effect of soil pH rather than the interaction effect of soil pH and fertilizer treatments on functional gene composition involved in microbial energy metabolisms. Furthermore, we determined nitrogen metabolism most affected by soil pH followed by the sulfur metabolism, methane metabolism, and carbon fixation. When relative abundances of microbial genes were averaged among fertilizer treatments, high soil pH increased gene abundance potentially associated with amino acid synthesis, while low pH increased gene abundance related to denitrifiers denitrification. N₂O emissions were higher under high soil pH conditions, suggesting a small contribution of denitrifiers to N₂O emissions. Among the fertilizer treatments, urea differently impacted gene abundance associated with N₂O and CH₄ emission depending on soil pH. This study provides an overview of microbial metabolic pathways influenced by soil pH and fertilizer treatments, specifically focusing on the microbial functions associated with soil environmental processes.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"208 ","pages":"Article 105992"},"PeriodicalIF":4.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143526992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assembly mechanisms of soil testate amoeba metacommunities: Insights from co-occurrence patterns and the critical role of null model selection","authors":"Qihan Zhu ,&nbsp;Aleksandr A. Ivanovskii ,&nbsp;Edward A.D. Mitchell ,&nbsp;Alexei A. Aleinikov ,&nbsp;Alexander A. Komarov ,&nbsp;Andrey N. Tsyganov ,&nbsp;Satoshi Shimano ,&nbsp;Olga V. Smirnova ,&nbsp;Donghui Wu ,&nbsp;Yuri A. Mazei","doi":"10.1016/j.apsoil.2025.105999","DOIUrl":"10.1016/j.apsoil.2025.105999","url":null,"abstract":"<div><div>Co-occurrence analyses were originally employed to explore community assembly mechanisms driven by species interaction, especially competition. Here, we applied co-occurrence analysis based on community null models to explore metacommunity assembly mechanisms of soil testate amoeba communities in two contrasted environmental settings in the Ural Mountains: 1) within a single type of microbiotope (<em>Picea obovata</em> sub-crown soil litter microbiotope) and 2) in the most typical microbiotopes from each vegetation type across the landscape. The former revealed random co-occurrence pattern that we interpret as evidence for the mass effect mechanism. The latter revealed segregated non-random co-occurrence pattern that we interpret as evidence for the species sorting mechanism. We tested several null models which differ in their underlying assumptions regarding ecological features of sites and species, and our findings underscore the crucial role of a reasonable choice of a null model for subsequent ecological inferences. In terms of site treatment, we recommend assessing species richness variability among sites to substantiate the choice of a specific null model. At the species features side, we base our choice of a specific null model on the species occurrence evidence.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"208 ","pages":"Article 105999"},"PeriodicalIF":4.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143526990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Responses and microbial mechanisms of greenhouse gas emissions and multifunctionality of soils at different elevations in Changbai Mountain under warming conditions","authors":"Yujuan Kang ,&nbsp;Qiang Guan ,&nbsp;Haitao Wu","doi":"10.1016/j.apsoil.2025.105972","DOIUrl":"10.1016/j.apsoil.2025.105972","url":null,"abstract":"<div><div>Mountains experience vertical variations in forest types and present different vegetation zones due to changes in topography and climate. Mountain environments are particularly sensitive to the effects of global warming, which has resulted in changes in the distribution of vegetation zones. Therefore, soil greenhouse gas emissions and their mechanisms in different vegetation zones under climate warming require further investigation. In this study, we conducted in-situ gradient shift warming experiments to evaluate the response of soil greenhouse gas emissions (GHGs) and multifunctionality in Changbai Mountain. Our results showed that warming increased soil CO₂ and N₂O emissions while enhancing CH₄ uptake in all four vegetation zones. The responses of soil CO₂ and N₂O emissions, as well as global warming potential, were more pronounced in coniferous forests. The sensitivity of CH₄ uptake to warming was highest in mixed coniferous-broadleaf forests. Microbial Shannon diversity was crucial for explaining the variations in CO₂ and N₂O emissions, whereas NO₃⁻-N levels primarily explained the variations in CH₄ uptake. Metagenomic analysis indicated that the functional profiles of microbial communities associated with soil carbon and nitrogen cycles changed across the four vegetation zones due to warming. Furthermore, climate warming decreased the multifunctionality of the mixed coniferous-broadleaf forest zone. PLS-PM revealed that microbial diversity and soil properties negatively impacted soil greenhouse gas emissions and positively affected soil multifunctionality, while warming positively influenced functional genes that are related to carbon and nitrogen cycles. These findings enhance our understanding of how climate warming impacts soil GHGs, carbon and nitrogen cycling, and ecosystem functions in mountain ecosystems, and are essential for mountain ecosystem management.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"208 ","pages":"Article 105972"},"PeriodicalIF":4.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143519326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ericaceous shrub encroachment influences the structure of soil fungal communities in subalpine grasslands","authors":"Camille Marchal ,&nbsp;Elena Tello-García ,&nbsp;Lucía Laorden-Camacho ,&nbsp;Marie-Noëlle Binet ,&nbsp;Karl Grigulis ,&nbsp;Marie-Pascale Colace ,&nbsp;Sophie Périgon ,&nbsp;Cindy Arnoldi ,&nbsp;Delphine Rioux ,&nbsp;Christian Miquel ,&nbsp;Frédéric Laporte ,&nbsp;Christiane Gallet ,&nbsp;Christelle Gonindard-Melodelima ,&nbsp;Georg Leitinger ,&nbsp;Bello Mouhamadou ,&nbsp;Sandra Lavorel","doi":"10.1016/j.apsoil.2025.105985","DOIUrl":"10.1016/j.apsoil.2025.105985","url":null,"abstract":"<div><div>Shrub encroachment influences fungal communities, but detailed knowledge of this influence missing. Using DNA metabarcoding, measure of plant functional traits, soil properties and laccase activities, we studied soil fungal communities along an ericaceous shrub encroachment gradient in two similar subalpine sites (Stubai, Austria and Lautaret, France) that differ in their shrub composition. Fungal taxonomic richness, driven by evergreen shrub cover and soil P, was 1.4 times lower in Stubai than Lautaret. Shrub density affected community taxonomic turnover only at Lautaret, with a qualitative change between 32 % and 63 % cover. Across sites, soil parameters were the primary determinants of fungal community turnover, along with shrub cover, herbaceous biomass and plant functional traits related to tissue quality. At Stubai, fungal community turnover responded to <em>Calluna vulgaris</em> cover, community weighted mean (CWM) lignin and soil properties (P, pH). In contrast, at Lautaret, the change in fungal community composition between low and high shrub cover was significantly explained by shrub cover, herbaceous biomass, plant traits (community-level C:P and herbaceous leaf dry matter content: LDMC) and soil pH. These differences in fungal community turnover translated to varying abundances of fungal functional groups between and within sites. In particular, high shrub cover was associated with a sharp decrease (12 times less) in ectomycorrhizal fungi and a sharp increase (1.3 times more) in saprophytes. Soil laccase activities were low and constant in Stubai, not exceeding 60 nmol. Min⁻¹. μg protein⁻¹ but were higher and decreased with increasing shrub density, from 281 to 125 nmol. Min⁻¹. μg protein⁻¹ at Lautaret. Our study demonstrates a significant impact of shrub functional composition on fungal communities, related to shrub density and functional traits associated with tissue quality. Such changes are expected to alter the biogeochemical functioning of subalpine grasslands.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"208 ","pages":"Article 105985"},"PeriodicalIF":4.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143526991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Symmetric responses of microbial carbon and nutrient limitations to warming and cooling in boreal forests: Insights from a transplant experiment","authors":"Sen Lu ,&nbsp;Dongdong Zhang ,&nbsp;Shuanglin Zhao ,&nbsp;Ziqiao Yang ,&nbsp;Ying Zhang ,&nbsp;Lili Yang ,&nbsp;Gongxiu He ,&nbsp;Li Ji","doi":"10.1016/j.apsoil.2025.105978","DOIUrl":"10.1016/j.apsoil.2025.105978","url":null,"abstract":"<div><div>Soil extracellular enzymes in boreal forests are considered more susceptible to climate change, which might result in potential changes in carbon (C) stocks and available nutrients. Despite mounting evidence has proven that warming (duration and magnitude) has a profound effect on extracellular enzyme activities (EEAs), little consideration has been given to the symmetric and asymmetric effects of warming and cooling on EEAs, as well as the limitations posed by microbial metabolism. Here, we reciprocally transplanted field soil monoliths from warm and cold sites with a short-term manipulated experiment (15 months) in boreal forests to stimulate climate change (varied 2.09 °C). This study aims to unravel divergent responses of EEAs to cooling and warming, and evaluate the metabolic limitation of soil microbes via ecoenzymatic stoichiometry. The results showed that the activities of 1,4-β-glucosidase, 1,4-β-<em>N</em>-acetyl-glucosaminidase and acid phosphatase were increased by 36.4 %, 77.9 % and 29.3 % under the warming regime, respectively. The symmetric variations of EEAs (excluding leucine aminopeptidase) were detected between cooling and warming regimes. In addition, warming had a slight alleviation of soil C limitation on microbial metabolism, whereas nutrient limitations were not changed by any stimulated climate change regimes. The variations in C limitations induced by warming/cooling were mainly driven by the microbial biomass, C- and phosphorus- acquiring enzyme activities, which were mediated by soil pH, macronutrients and dissolved organic nutrients, respectively. Collectively, our findings highlight the symmetric effects of stimulated warming and cooling on C limitation of soil microbial metabolism.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"208 ","pages":"Article 105978"},"PeriodicalIF":4.8,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143510075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The different pathways of microbial regulation of organic carbon turnover in the topsoil and subsoil of coastal saline soil after long-term stubble return and subsoiling","authors":"Xunya Su ,&nbsp;Le Zhang ,&nbsp;Hao Meng ,&nbsp;Jiaxue Zhao ,&nbsp;Han Wang ,&nbsp;Lunxiao Shang ,&nbsp;Qiyuan Guo ,&nbsp;Yupeng Xing ,&nbsp;Xianliang Song ,&nbsp;Xuezhen Sun ,&nbsp;Xiaopei Zhang ,&nbsp;Xiaoli Tian ,&nbsp;Lili Mao","doi":"10.1016/j.apsoil.2025.105970","DOIUrl":"10.1016/j.apsoil.2025.105970","url":null,"abstract":"<div><div>Long-term stubble return and subsoiling increased the soil organic carbon (SOC) in coastal saline soil; however, the changes in SOC components and their regulatory role of microorganisms in this process remain unclear. Therefore, a 9-year experiment assessed stubble management (removal or return) and tillage (non-subsoiling or subsoiling) effects on SOC components and microbial communities to clarify microbial regulation of SOC turnover. Results showed that stubble return and subsoiling improved the physicochemical properties of the topsoil (0–20 cm) and subsoil (20–40 cm), and increased SOC and its components, including dissolved organic carbon (DOC) by 160 %, easily oxidizable carbon (EOC) by 59 %, microbial biomass carbon (MBC) by 171 %, particulate organic carbon (POC) by 103 %, and mineral-associated organic carbon (MOC) by 22 %. In the subsoil, stubble management significantly increased DOC, EOC, MBC, POC, and MOC by 21 %, 62 %, 27 %, 72 %, and 37 %, respectively. Through high-throughput sequencing and Biolog-ECO microplate, we found significant changes in the structure and metabolic function of microbial communities after stubble return and subsoiling. Notably, stubble return and subsoiling altered the microbial regulatory pathways in SOC turnover in different soil layers. In the topsoil, microorganisms primarily utilize EOC to convert it into POC, while the direct adsorption of DOC by minerals serves as the main pathway for MOC synthesis. In the subsoil, DOC serves as the primary carbon source for microorganisms, leading to the conversion to MBC and the promotion of MOC formation. Our results highlight that stubble return and subsoiling can optimize the microbial community structure and metabolic function and increase SOC sequestration with different regulation pathways in SOC component turnover, and the measures can be effective measures to improve the status of coastal saline soil.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"208 ","pages":"Article 105970"},"PeriodicalIF":4.8,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143511440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}