Applied Soil Ecology最新文献

{"title":"Soil nitrogen availability regulates fungal necromass contribution to soil organic carbon during vegetation restoration","authors":"Tingting Sun ,&nbsp;Tian Zhang ,&nbsp;Jie Zhou ,&nbsp;Zhufeng Wang ,&nbsp;Zhigang Huang","doi":"10.1016/j.apsoil.2025.106279","DOIUrl":"10.1016/j.apsoil.2025.106279","url":null,"abstract":"<div><div>Microbial necromass is an important component of soil organic carbon (SOC) and increases with vegetation restoration. Yet, the quantitative evaluation of the drivers of microbial necromass accumulation during restoration on large scales remains unclear. Here, a global meta-analysis of 462 paired peer studies (covering temperate, tropical, and subtropical zones) was firstly conducted to quantitatively evaluate the response of fungal vs. bacterial necromass contributions across restoration strategies. On average, vegetation restoration significantly increased microbial necromass C content by 67 % (95 % CIs: 56 %–79 %), with a greater increase of 115 % (95 % CIs: 97 %–134 %), 75 % (95 % CIs: 63 %–88 %), and 69 % (95 % CIs: 57 %–81 %) in natural restoration, forest soils, and &gt;20 years restoration compared to artificial, grassland, and &lt;20 years restoration, respectively. The constant contributions of microbial necromass to SOC were due to the offset of fungal (10 %) and bacterial necromass (−9 %) contributions to SOC. This observation implies the high consistency of SOC and microbial necromass, as well as the importance of fungal-dominated community in SOC sequestration. The restoration effects on microbial necromass and C sequestration were dominantly explained by soil C/N (nitrogen) as soil C/N &gt; 15 enhanced microbial biomass (MB) conversion to necromass (ΔMNC/MB = 1.21), driving SOC sequestration. This indicates that vegetation restoration might be an efficient approach for microbial-derived C accumulation in N-limited soils (soil C/N &gt; 15), such as ecologically vulnerable regions. Overall, this study indicates that long-term natural forest restoration is recommended for microbial necromass accumulation, and highlights the importance of soil N availability in improving soil microbial-derived C sequestration in response to global land use changes.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"213 ","pages":"Article 106279"},"PeriodicalIF":4.8,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144330432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Looking underground: How urban lawn mowing frequency affects soil mesofauna feeding activity","authors":"Joanna Kajzer-Bonk ,&nbsp;Beata Klimek ,&nbsp;Dorota Lachowska-Cierlik ,&nbsp;Łukasz Musielok","doi":"10.1016/j.apsoil.2025.106271","DOIUrl":"10.1016/j.apsoil.2025.106271","url":null,"abstract":"<div><div>The management of green areas in urban environments is of increasing interest. Reducing the frequency of lawn mowing has many advantages; however, it is not clear how it affects soil mesofauna, an important element of terrestrial ecosystems. This study was based on a field experiment conducted in public green areas on the campus of the Jagiellonian University in Kraków (Poland). In the experiment, 64 experimental plots of average size 130 m² were established and combined into 32 pairs of adjacent plots, where one plot was mown once a year and the other plot in the pair was mown under a different regime, including one mowing every two years and four, six, and eight times a year (<em>n</em> = 8). Vegetation was cut to a height of 6 cm using a petrol-powered mowing tractor with a biomass collector. The effect of mowing on the feeding activity (FA) of soil mesofauna was examined in the field four times during the two-year experiment using the bait-lamina test. The same feeding substrate and depth of bait lamina strips in the soil was used throughout the experiment. Mean FA in individual plots was 2.95 % (± 1.24 %) per day and was not affected by mowing frequency, soil temperature, or soil moisture. However, more frequent mowing (four, six, and eight times per year) resulted in a steeper decline in FA with soil depth, and the effect was most significant for mowing eight times per year, amounting to an additional 15 % decline in FA along the vertical soil gradient sampled (8 cm of soil depth). Therefore, the recommended frequency of mowing should follow that recommended for other groups of organisms, including plants, that is, once or twice a year. Reduced mowing frequency allows better functioning of the anthroposols for naturalisation by activating deeper soil layers.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"213 ","pages":"Article 106271"},"PeriodicalIF":4.8,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144330407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Arbuscular mycorrhizal fungi reshape the stability and complexity of micro-food webs in the shrubland soils of a dryland ecosystem","authors":"Guannan Zhu ,&nbsp;Haojun Nong ,&nbsp;Shuyi Fang ,&nbsp;Bin He ,&nbsp;Shugao Qin ,&nbsp;Yuqing Zhang","doi":"10.1016/j.apsoil.2025.106270","DOIUrl":"10.1016/j.apsoil.2025.106270","url":null,"abstract":"<div><div>Soil micro-food webs constitute critical biological networks sustaining terrestrial ecosystem functionality. The structural dynamics (including stability and complexity) in soil micro-food webs are important for regulating nutrient flow within the soil, thereby influencing the nutrient supply to plants. Xerophytic shrubs in dryland ecosystems frequently establish obligate symbiotic associations with arbuscular mycorrhizal fungi (AMF), forming critical ecological partnerships for nutrient acquisition. However, critical knowledge gaps persist in understanding how AMF modulate micro-food web architecture and regulate stability-complexity dynamics in dryland soils, particularly regarding their spatial-temporal variability and hydrological dependencies. We established a controlled culture system for the xerophytic shrub <em>Artemisia ordosica</em> and AMF to investigate the effects of AMF on the stability and complexity of soil micro-food webs. AMF inoculation and soil water content (SWC) treatments significantly altered the community structure of bacteria, protists, and nematodes in both rhizosphere and bulk soils, as well as fungi in rhizosphere soils, but showed limited impact on fungal communities in bulk soils. AMF enhanced rhizosphere stability by strengthening fungal-bacterial synergies (+63 % positive interaction), and AMF regulated the soil micro-food web structure through the “bottom-up” effect (significant alterations among lower trophic levels influencing higher trophic levels). Conversely, extreme drought shifted AMF's role to “top-down” effect (opposite to “bottom-up” effect) destabilizing rhizosphere networks via amplified protist-nematode antagonism (+235 % negative interactions). The regulation of soil micro-food web stability and complexity by AMF under well-watered and extreme drought conditions is complex in bulk soils. This study identified critical hydrological thresholds (3 % SWC) governing AMF functional transitions, advancing mechanistic insights into mycorrhizal regulation of nutrient cascades in arid soils. Although our experimental system may amplify AMF functions compared to natural conditions, these findings advance mechanistic understanding of trophic interactions in dryland soils.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"213 ","pages":"Article 106270"},"PeriodicalIF":4.8,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144330406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The increase of microbial diversity enhances the compensatory thermal adaptation of soil microbial respiration","authors":"Zhengyi Huang,&nbsp;Yangui Su,&nbsp;Sinuo Lin,&nbsp;Guopeng Wu,&nbsp;Hao Cheng,&nbsp;Jingyi Yan,&nbsp;Gang Huang","doi":"10.1016/j.apsoil.2025.106263","DOIUrl":"10.1016/j.apsoil.2025.106263","url":null,"abstract":"<div><div>Accurately predicting the feedback mechanisms of soil carbon (C) pool in response to warming hinges on our understanding of the thermal response of microbial respiration. However, how the thermal response of microbial respiration varies along climate gradients has not been systematically evaluated. In this study, using soils from natural forests along a 3800<!--> <!-->km transect across China, we assayed microbial respiration response to temperature curves under 10 measurement temperatures for all samples after 6 months of incubation at two different temperatures (10 and 30 °C). Meanwhile, based on the macromolecular rate theory, we quantified thermal traits (T_opt (temperature optimum) and T_inf (inflection point)) and the thermal response of microbial respiration across a latitudinal-scale forest transect. Our findings reveal a shift in microbial respiration response to temperature curves towards higher incubation temperatures, accompanied by increases in both T_opt and T_inf, indicating a compensatory thermal adaptation of microbial respiration (CTA). The magnitude of CTA (the response ratio of thermal traits under incubation temperatures) exhibited a U-shaped relationship with mean annual temperature (MAT) along the transect and was associated with shifts in microbial biomass, bacterial richness, bacterial and fungal dominant community. Further analysis revealed that bacterial richness explained the maximum variation of CTA. For microbial properties, bacteria richness did not change significantly with MAT, while fungi richness increased linearly with increasing MAT. Our findings emphasize the consistent compensatory thermal adaptation of microbial respiration in forest soils and the critical link between microbial communities and thermal adaptation, with implications for better characterizing soil climate-C feedback under warming.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"213 ","pages":"Article 106263"},"PeriodicalIF":4.8,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144322504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rhizosphere priming mechanisms on soil organic carbon decomposition differ among exudate components and are regulated by mycorrhizal type: Insights from a temperate forest in northeast China","authors":"Gang Liu ,&nbsp;Faustine Mecksedeck Mbonde ,&nbsp;Xiuwei Wang","doi":"10.1016/j.apsoil.2025.106276","DOIUrl":"10.1016/j.apsoil.2025.106276","url":null,"abstract":"<div><div>Root exudate-derived labile carbon (C) inputs can lead to a strong short-term change in microbial mineralization of rhizosphere soil organic carbon (SOC), which is termed the rhizosphere priming effect (RPE). In this study, we added three exudate components surrogates (glucose, oxalic acid, and glycine) to rhizosphere soils collected from eight tree species, exploring how tree species variation modulated the response of RPE to addition of root exudate components. Our results showed that adding glucose and glycine enhanced soil C mineralization through biotic mechanisms, while adding oxalic acid enhanced soil C mineralization through abiotic mechanisms. Compared with control, adding glucose, oxalic acid, and glycine additions increased cumulative C mineralization by 94.1 %, 87.6 %, and 26.8 %, respectively (eight species combined). Moreover, the increase in rhizosphere cumulative C mineralization induced by adding glucose and oxalic acid was greater in arbuscular mycorrhizal (AM) soils (5.33 and 5.12 mg CO₂-C g⁻¹ soil) than in ectomycorrhizal (ECM) soils (4.82 and 4.69 mg CO₂-C g⁻¹ soil). However, the relationships among enzyme activity, microbial biomass, and C mineralization depended on tree species, and different tree species have an inconsistent biotic driving mechanism for RPE. There was no linear relationship between cumulative C mineralization and bioavailable C and N among soils. Instead, soil/microbial C:N imbalances and easily oxidizable carbon (EOC) content were crucial factors regulating RPE among temperate forest tree species. Taken together, the effect of root exudates on soil C mineralization was component-specific, and RPE was driven by both root exudate components and mycorrhizal types.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"213 ","pages":"Article 106276"},"PeriodicalIF":4.8,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144322505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plant growth-promoting bacteria reshape rhizosphere microbial networks and biochemical properties to drive sustainable Zea mays growth","authors":"Yingying Cheng,&nbsp;Ying Ma","doi":"10.1016/j.apsoil.2025.106275","DOIUrl":"10.1016/j.apsoil.2025.106275","url":null,"abstract":"<div><div>Plant growth-promoting bacteria (PGPB) inoculants are crucial for sustainable agriculture, enhancing ecological balance and crop yields. Soil microbial communities are integral to maintaining soil fertility and ecological stability. However, the precise impacts of exogenous PGPB inoculants on the intricate relationships within rhizosphere microbial communities remain underexplored under phosphorus (P)-deficiency conditions. This study investigated the relationships between laboratory-screened endophytic and rhizosphere PGPB and microbial communities in a <em>Zea mays</em> pot experiment, assessing their effects on soil biochemical properties and plant physiological parameters. Results revealed that PGPB inoculation significantly influenced the relative abundance of rhizosphere microbial communities, enhancing the modularity of indigenous bacterial and differential microbiota. Notably, highly connected nodes within microbial community modules suggested enhanced functional interactions. Kruskal-Wallis rank-sum tests for intergroup differences, network analysis of differential microbiota, and PICRUSt2 functional prediction demonstrated that exogenous PGPB inoculants significantly increased the relative abundance of microbiota associated with carbon and nitrogen metabolism, including <em>Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium</em> sp., <em>Ciceribacter</em> sp., and <em>Azospirillum</em> sp. Further analysis using db-RDA, correlation network maps, and partial least squares structural equation modeling revealed the close relationships between these microbial communities and key soil nutrient factors (e.g., soil organic matter, available phosphorus), soil enzyme activities (e.g., acid phosphatase, alkaline phosphatase, urease, cellulase), and plant physiological indicators (e.g., photosynthetic rate, soluble sugar, and soluble protein content). Our study demonstrates that PGPB inoculation enhances beneficial bacteria, microbial interactions, and rhizosphere soil properties, supporting <em>Z. mays</em> growth and development under P-deficiency conditions. These findings improve our understanding of PGPB's ecological roles and growth-promoting mechanisms, encouraging their broader use in sustainable agriculture.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"213 ","pages":"Article 106275"},"PeriodicalIF":4.8,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144322438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rhizosphere synergy: The role of endogeic earthworms in nutrient cycling, plant growth, and soil organic matter stabilization","authors":"Anna Cibulková,&nbsp;Hana Šantrůčková,&nbsp;Eva Kaštovská","doi":"10.1016/j.apsoil.2025.106272","DOIUrl":"10.1016/j.apsoil.2025.106272","url":null,"abstract":"<div><div>Living plant roots play a crucial role in stabilizing soil organic matter (SOM), which in turn influences overall soil function in ecosystems. Sequestration of SOM is also mediated by earthworms, which facilitate the transformation of older SOM and fresh plant material into more stable forms. While this role is well documented for earthworms feeding on litter, the interaction between endogeic, soil-dwelling earthworms, root-derived inputs to the soil, such as rhizodeposition, and the rhizosphere microbiome is even less known. In an eight-week laboratory experiment, we investigated the interaction between the endogeic earthworm <em>Aporrectodea caliginosa</em> and the roots of maize (<em>Zea mays</em>) using rhizoboxes with soil differing from the maize in its ¹³C natural abundance to determine the fate of rhizodeposition, the effects on nutrient availability, microbial activity and SOM, especially the dynamics of particulate organic matter (POM) and mineral-associated organic matter (MAOM), as well as the formation of stable aggregates in the rhizosphere. We found that earthworms promote plant growth by accelerating nitrogen recycling and increasing nitrate availability, to a lesser extent phosphorus uptake by plants, and to a large extent phosphorus accumulation in the rhizosphere microbial community. Earthworm activities, which led to increased plant biomass and rhizodeposition, stimulated microbial processes in the rhizosphere, accelerated SOM turnover, improved aggregate stability and appeared to favour the formation of stable MAOM. These results underline the positive influence of the interaction between soil-dwelling earthworms, plant roots and microorganisms in the rhizosphere on SOM stabilization and nutrient cycling, and thus on overall soil quality.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"213 ","pages":"Article 106272"},"PeriodicalIF":4.8,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144330405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rhizosphere transplants from Guinea grass support the yield and modulate the microbiota of chili and rice root systems depending on the plant's variety and growth stage","authors":"Lorenzo Vergani ,&nbsp;Mekhala Chandrasekara ,&nbsp;Chathurika Wanninayake ,&nbsp;Francesca Mapelli ,&nbsp;Sanath Hettiarachi ,&nbsp;Sara Borin","doi":"10.1016/j.apsoil.2025.106273","DOIUrl":"10.1016/j.apsoil.2025.106273","url":null,"abstract":"<div><div>Biofertilization by rhizosphere microbiome transplant (RMT) is an emerging approach of plant microbiome engineering, for its potential to reduce the input of synthetic chemicals, hence preserving soil ecosystem and human health. However, its feasibility and outcomes need deeper investigation through field studies. This work aimed at evaluating the efficacy and the ecological impact on soil microorganisms of a biofertilizer that could be self-produced by farmers in Sri Lanka through RMT from Guinea grass (<em>Panicum maximum</em>), a widespread weed able to adapt to harsh conditions. Root wash (RW) and arbuscular mycorrhizae (AMF) obtained from the root system of <em>Panicum</em> were separately supplied to chili pepper and two local varieties of rice, Suwadel and Kuruluthuda. Decreasing doses of chemical fertilizer were also applied, combined with the inocula or as separate controls. In chili and in rice var. Suwadel all the biofertilization treatments improved the crop productivity compared to the non-treated controls or to the plants supplemented only with the minimum dose of chemical fertilizer. RW and AMF applied alone or supplied with 50 % of the optimal fertilizer dose resulted in yields comparable to 100 % chemical fertilization, suggesting the potential to reduce its input by half. Microbiome transplant showed an impact on bacterial and fungal communities at flowering stage, with the enrichment of Bacillaceae, Exiguobacteraceae, Micrococcaceae, Trichocomaceae and Aspergillaceae compared to non-treated plants. Our results indicate promising results of RMT in terms of crop yield improvement in field conditions, with structural changes in the rhizosphere microbiome related to the recruitment of beneficial microorganisms.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"213 ","pages":"Article 106273"},"PeriodicalIF":4.8,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144330577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Land subsidence caused by underground coal mining resource development alters soil properties and disrupts bacterial community assembly mechanisms in wheat fields: A case study of mining ages of 16, 31, and 40 years","authors":"Shuo Li ,&nbsp;Wen Ge ,&nbsp;Yongtao Wang ,&nbsp;Zizhu Wang ,&nbsp;Hua Cai ,&nbsp;Lei Zhang","doi":"10.1016/j.apsoil.2025.106262","DOIUrl":"10.1016/j.apsoil.2025.106262","url":null,"abstract":"<div><div>Underground coal mining induced subsidence alters soil properties, causing nutrient loss and reduced fertility. Soil microbial communities, which are highly sensitive to environmental changes, play a crucial role in nutrient cycling within these ecosystems. However, the dynamics of microbial succession, community assembly, and species coexistence in subsidence-affected wheat fields remain underexplored. To address this knowledge gap, this study employs 16S rRNA gene sequencing, alongside linear mixed-effects models, neutral models, and phylogenetic null models, to investigate bacterial community characteristics and assembly mechanisms in wheat fields soils at varying depths (0–20, 20–40 and 40–60 cm) near subsided lakes (within 100 m) with varying mining ages (16, 31 and 40 years). The results indicate that land subsidence increases soil moisture and alters the distribution of potassium, nitrogen, phosphorus. This process significantly (<em>P</em> &lt; 0.001) enhances the similarity of bacterial communities between the middle and deep layers while emphasizing their differences from the surface layer. Over time, the long-term dynamics of conditionality rare or abundant taxa (CRAT) in the bacterial community emphasized the enhanced ability of the bacterial community to oxidise ammonia, promote the rise of soil organic matter content and improve soil aggregate stability. At greater depths, the communities show advantages in nitrification, denitrification, and the efficient utilization of limited and complex organic substrates. Additionally, with increasing depth, deterministic processes significantly influence bacterial community composition (especially CRAT), making the co-occurrence network more dependent on a few core taxa, a trend that becomes more evident as mining age increases. In contrast, communities shaped by long-term environmental fluctuations are mainly driven by stochastic processes, further confirming that increased soil moisture due to land subsidence enhances the potential for taxa dispersal. This study highlights the necessity of land reclamation and sustainable agricultural management to restore soil ecology in subsidence-affected areas.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"213 ","pages":"Article 106262"},"PeriodicalIF":4.8,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144330404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced microbial network stability and biogeochemical cycles in saline-alkali soil through simplified prokaryotes and complex fungal networks","authors":"Ruihong Wang ,&nbsp;Hao Qin ,&nbsp;Zhijian Shi ,&nbsp;Mengben Wang ,&nbsp;Junjian Li","doi":"10.1016/j.apsoil.2025.106245","DOIUrl":"10.1016/j.apsoil.2025.106245","url":null,"abstract":"<div><div>Soil salinization has rapidly become a critical global environmental issue in the current century. Understanding the structure of microbial networks and their interactions with biogeochemical cycles is vital to maintaining the stability of microbial communities and predicting ecosystem responses to salinization under climate change scenarios. Using metagenomic sequencing focuses on analyzing microbial community characteristics, as well as the function genes responsible for the cycles of carbon (C), nitrogen (N), phosphorus (P), and sulfur (S), in four distinct natural saline-alkali gradients: Non-saline, Low salinity, Medium salinity, and High salinity. The result revealed that salinity significantly alters the structures of bacterial, fungal, and archaeal communities and influences the functional genes related to the biogeochemical cycles. Notably, the increase of relative abundance in Proteobacteria (0.20, 0.30, 0.36, 0.38) with salinity, suggest its utility as a salinity indicator. Linear regression model revealed a significant negative correlation between salinity and the network complexity of prokaryotic, with higher network complexity does not favor the structural stability. In contrast, fungi network complexity positively correlated with salinity and stability. Additionally, while the complexity of prokaryotic networks significant negatively correlated with the metabolic potential of C, N, and S cycles, fungal showed a significantly positive correlation with P cycling. Random forest results identified salinity as the top driver of microbial network complexity (bacteria: 8.28 %; fungi: 7.22 %, archaea: 9.28 %). These insights suggest that the structural differences between prokaryotes and fungi result in varying responses to salinity, network structure, and elemental cycles. The interplay between simplified prokaryotic networks and more complex fungal networks could enhance microbial network stability and improve biogeochemical cycling. This study newly identifies the divergent responses of prokaryotic and fungal networks to salinity, challenging previous assumptions about uniform microbial responses. Therefore, maintaining the appropriate complexity of belowground communities is essential for the effective management of saline-alkali ecosystems and sustainable agricultural development.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"213 ","pages":"Article 106245"},"PeriodicalIF":4.8,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144322437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}