Applied Soil Ecology最新文献

{"title":"Cover crop diversity improves the biochemical, physical and microbiological attributes of soil under long-term no-tillage","authors":"Andressa Selestina Dalla Côrt ,&nbsp;Leandro Pereira Pacheco ,&nbsp;Bruno Moço Tessarolli ,&nbsp;Éder Rodrigues Batista ,&nbsp;Thais Rodrigues Magalhães Guedes ,&nbsp;Vinícius Hipólito Lopes de Resende ,&nbsp;Fabiano André Petter ,&nbsp;Edicarlos Damacena de Souza ,&nbsp;Jayme Ferrari Neto ,&nbsp;João Paulo Gonsiorkiewicz Rigon ,&nbsp;Carlos Alexandre Costa Crusciol","doi":"10.1016/j.apsoil.2025.106494","DOIUrl":"10.1016/j.apsoil.2025.106494","url":null,"abstract":"<div><div>The advancement of no-tillage systems for grain production requires techniques promoting crop diversity and enhancing plant interactions with soil physical and biochemical attributes. This study aimed to evaluate the potential of cover crops to improve the microbiological, biochemical and physical attributes of soil and increase soybean yield under a long-term no-tillage system in the Cerrado region of Mato Grosso, Brazil. The experiment was installed in Rondonópolis-MT in 2013/14, and this work presents the data from the 2017/18 to 2020/21 crop seasons. The treatments comprised the following sets of second-crop systems: monocropped (MC) (fallow), <em>Crotalaria spectabilis</em> (CS1), <em>Pennisetum glaucum</em> (CS2), <em>Urochloa ruziziensis</em> (CS3), <em>Cajanus cajan</em> (CS4), and MIX (<em>C. spectabilis</em> + <em>P. glaucum</em> + <em>U. ruziziensis</em> + <em>C. cajan</em>). The total C stock was highest in CS3, and the stock of particulate C was highest in CS2, CS3 and MIX, also showing lower penetration resistance at all depths. Soil enzymatic activity and microbial analyses demonstrated that MIX was 60 % and 67 % higher microbial biomass C and N, respectively than in MC. Soybean grain yield was highest in CS3 and MIX, increasing approximately 620 kg ha⁻¹ considering MC. The diversity of MIX favored soybean yield and carbon sequestration, evidenced by improvements in soil penetration resistance, carbon from biomass and soil microbial biomass. <em>U. ruziziensis</em> enhanced the activity of enzymes degrading organic residues, particularly <em>β</em>-glucosidase, and positively influenced carbon accumulation in the system. MC does not improve the soil properties and soybean yield in the Cerrado.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"215 ","pages":"Article 106494"},"PeriodicalIF":5.0,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145155216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Amplification effects of AM fungus and rhizobacteria on carbon efficiency in wheat-soil system under drought stress via priming rhizosphere activities","authors":"Muhammad Maqsood Ur Rehman ,&nbsp;Ling Zhao ,&nbsp;Sidra Khattak ,&nbsp;Yun-Li Xiao ,&nbsp;Awais Iqbal ,&nbsp;Wasim Khan ,&nbsp;Muhammad Abrar ,&nbsp;Zheng-Guo Cheng ,&nbsp;Shi-Sheng Li ,&nbsp;Asfa Batool ,&nbsp;Ying Zhu ,&nbsp;You-Cai Xiong","doi":"10.1016/j.apsoil.2025.106467","DOIUrl":"10.1016/j.apsoil.2025.106467","url":null,"abstract":"<div><h3>Background</h3><div>Arbuscular mycorrhizal fungi (AMF) and plant growth-promoting rhizobacteria (PGPR) are essential for improving crop yield and drought adaptation. Yet, their combined potential to increase carbon efficiency remains unexplored.</div></div><div><h3>Methods</h3><div>To address this issue, a growth environment-controlled experiment was conducted to investigate the synergetic impacts of <em>Rhizophagus irregularis</em> (strain GSICC 63801) and <em>Bacillus amyloliquefaciens</em> (strain GSICC 32826) on carbon efficiency in the wheat-soil system under the well-watered (WW; 80 % field water capacity, FWC), moderate water stress (MWS; 50 % FWC), and severe water stress (SWS; 35 % FWC), synergized with machine learning.</div></div><div><h3>Results</h3><div>The data indicated that the joint AMF-PGPR inoculation showed significantly stronger soil carbon sequestration than the sole inoculation (<em>n</em> = 3, <em>p</em> &lt; 0.05), which became more evident along with drought stress intensity. AMF-PGPR co-inoculation significantly improved soil organic carbon by 5.42 % under SWS compared to CK (non-inoculation) due to a significant increase in microbial biomass carbon, particulate organic carbon, easily oxidizable carbon, and dissolved organic carbon. AMF-PGPR co-inoculation significantly enhanced the activities of key carbon sequestering enzymes (Xylosidase, β-Glucosidase, and cellobiohydrolase), with increasing abundance of microbial communities, compared with the sole inoculation and CK. The AMF-PGPR combination improved carbon emission efficiency, CO₂ assimilation, and net carbon balance despite increased soil respiration. Increasing water use efficiency ultimately enhances wheat grain yield, particularly under SWS. Also, gradient boosting machine (MSE = 0.12, <em>R</em>^<em>2</em> = 0.99 training, <em>R</em>^<em>2</em> = 0.88 testing) outperformed random forest regression (MSE = 0.14, <em>R</em>^<em>2</em> = 0.95 training, <em>R</em>^<em>2</em> = 0.80 testing) in predicting SOC, validated by structural equation modeling (<em>R</em>^<em>2</em> = 0.86).</div></div><div><h3>Conclusion</h3><div>These results position the AMF-PGPR combination as a green solution to improve carbon efficiency in the wheat-soil system.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"215 ","pages":"Article 106467"},"PeriodicalIF":5.0,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145155136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Forest composition and litter quality shape bacterial community dynamics and functional genes during litter decomposition","authors":"Lin Chang ,&nbsp;Bo Li ,&nbsp;Kang Liu ,&nbsp;Wenjing Meng ,&nbsp;Yuemei Zhang ,&nbsp;Hui Sun ,&nbsp;Lin Huang","doi":"10.1016/j.apsoil.2025.106465","DOIUrl":"10.1016/j.apsoil.2025.106465","url":null,"abstract":"<div><div>Microbial community plays essential roles in forest nutrient cycling and ecosystem functioning, particularly through their involvement in litter decomposition. This study investigated bacterial dynamics over a three-year litter decomposition experiment in pure <em>Pinus thunbergii</em> forests and mixed <em>P. thunbergii</em>–<em>Liquidambar formosana</em> forests. We assessed bacterial community succession in two litter types (needle and twig) using high-throughput sequencing and functional gene analysis. The results showed that bacterial community richness, diversity, and evenness significantly increased over time, accompanied by a taxonomic shift from early dominance by Proteobacteria to late-stage dominance by Acidobacteria. Pine needle litter decomposed faster and supported more rapid microbial turnover than twig litter. Principal Coordinate Analysis (PCoA) and PERMANOVA revealed significant variation in bacterial community structure across decomposition stages and between litter types (<em>P</em> &lt; 0.05). Functional prediction indicated that chemoheterotrophy and nitrogen fixation were dominant functional guilds. Additionally, genes involved in denitrification and organic phosphorus mineralization were more abundant in twig litter in mixed forests during the third year of decomposition. These findings demonstrate that both forest composition and litter quality strongly influence bacterial community succession and functional potential. Mixed forests supported greater microbial functional diversity, highlighting their ecological value in sustaining nutrient cycling. This study provides valuable insights into bacterial contributions to litter decomposition and forest ecosystem processes.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"215 ","pages":"Article 106465"},"PeriodicalIF":5.0,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145155134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of temperature and moisture on the co-inoculation remediation of petroleum-polluted soil by bacteria and nematodes","authors":"Youqian Li ,&nbsp;Meng Na ,&nbsp;Jiajie Tang ,&nbsp;Shangqi Xu ,&nbsp;Jihai Zhou","doi":"10.1016/j.apsoil.2025.106490","DOIUrl":"10.1016/j.apsoil.2025.106490","url":null,"abstract":"<div><div>Petroleum contamination poses a serious global environment threat. Microorganisms have substantial potential for petroleum degradation and are environmentally friendly, but their effectiveness varies with environmental conditions. Nematodes can further enhance oil removal efficiency, but the mechanisms underlying their combined remediation with bacteria, particularly in response to environmental changes, remain unclear. To address this knowledge gap, this study investigated the effects of temperature and moisture on oil remediation by introducing nematodes into contaminated soil under three levels of temperature and moisture. Petroleum-contaminated soil at 22 °C and 50 % humidity served as the baseline treatment (SPN), with the following variations: lower temperature (14 °C, SPN_LT), higher temperature (30 °C, SPN_HT), lower moisture (40 %, SPN_LM), and higher moisture (60 %, SPN_HM). After 168 days, results indicated that nematodes consistently facilitated petroleum degradation across all tested conditions by modifying bacterial community structure and the relationships among bacterial genera. Notably, petroleum content was significantly lower in the SPN_HM and SPN_HT treatments, indicating that both elevated temperature and increased moisture enhance oil removal efficiency. Interestingly, the Shannon index increased significantly with rising moisture but decreased with increasing temperature. Principal coordinates analysis (PCoA) further indicated that temperature had a stronger influence on bacterial community structure than moisture. These findings suggest that temperature and moisture enhance oil remediation through distinct mechanisms. Furthermore, correlation and co-occurrence network analyses revealed that different petroleum-degrading bacterial genera formed separate network modules. These modules included both degradative and associated functional genera, with significant positive correlations, indicating that hydrocarbon breakdown is driven by diverse bacterial consortia composed of both degraders and supportive functional bacteria. In conclusion, our study demonstrates that increased moisture and temperature, combined with nematode addition, significantly improve petroleum degradation efficiency. This improvement is attributed to the interactions between nematodes and bacteria, highlighting the importance of both petroleum-degrading genera and their functional partners. Future research should focus on the roles of auxiliary functional bacteria and nematodes in promoting microbial biodegradation.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"215 ","pages":"Article 106490"},"PeriodicalIF":5.0,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145155133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Compared with plant diversity and soil fungal diversity, soil bacterial diversity drives ecosystem multifunctionality during the vegetation restoration process","authors":"Hengkang Xu ,&nbsp;Chao Chen ,&nbsp;Zhuo Pang ,&nbsp;Guofang Zhang ,&nbsp;Weiwei Zhang ,&nbsp;Haiming Kan","doi":"10.1016/j.apsoil.2025.106482","DOIUrl":"10.1016/j.apsoil.2025.106482","url":null,"abstract":"<div><div>Restoring vegetation enhances ecosystem multifunctionality (EMF). Species diversity, which includes plant, bacterial, and fungal diversity, plays a fundamental role in maintaining EMF. However, continuous monitoring of the effects of plant and microbial diversity on ecosystem functions across various vegetation restoration strategies remains insufficient. Over 5 years (2017–2021), we investigated the impact of different vegetation restoration methods—<em>Medicago sativa</em> (alfalfa) replanting (AF), <em>Bromus inermis</em> (smooth brome) replanting (SB), and natural restoration (CK)—on both aboveground EMF (AEMF, based on plant productivity) and belowground EMF (BEMF, based on nutrient cycling indicators) in degraded lands of North China. The results indicated that AF primarily enhances EMF by elevating AEMF, while SB predominantly improves EMF by enhancing BEMF. Regression analysis revealed that the EMF and BEMF of AF and SB treatments followed an initial increase, followed by a subsequent decline, with the progression of restoration time. Notably, bacterial diversity—rather than plant or fungal diversity—was positively correlated with EMF during the restoration process. The findings also highlight the dynamic relationship between bacterial community network stability and soil multifunctionality during vegetation restoration. The structural equation model indicates that pH has a direct negative impact on EMF and also indirectly regulates EMF by influencing microbial diversity. These findings enhance our understanding of how biodiversity relates to ecosystem functioning during vegetation restoration, to help develop more accurate and effective restoration strategies.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"215 ","pages":"Article 106482"},"PeriodicalIF":5.0,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145155132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decomposition of plant, fungal and tea litter in Picea abies forests with and without a history of clear-cutting","authors":"Rieke Lo Madsen ,&nbsp;Line Nybakken ,&nbsp;O. Janne Kjønaas ,&nbsp;Håvard Kauserud ,&nbsp;Johan Asplund","doi":"10.1016/j.apsoil.2025.106485","DOIUrl":"10.1016/j.apsoil.2025.106485","url":null,"abstract":"<div><div>Litter decomposition is coupled to carbon (C) sequestration through C release to the atmosphere, C transformation and nutrient release to the soil. We investigated if clear-cutting has long-term effects on this vital ecological process and consequently on C dynamics in boreal forests using twelve pairs of previously clear-cut and near-natural forests. Three litterbag experiments were conducted using (I) standardised spruce and bilberry litter, (II) melanised and non-melanised fungal necromass and (III) rooibos and green tea. We found weak and inconsistent effects of harvesting history, that did not depend on litter quality or mesofauna exclusion. Litter quality was more important in explaining net mass remaining for fungal necromass than for aboveground plant litter. Mesofauna exclusion had only marginal effects on initial litter decomposition. Results obtained with the highly standardised Tea Bag Index were not readily comparable to those of the plant litter or fungal necromass and we therefore question its use in this regional context. Further, we show that net mass or C remaining in the litterbags do not correlate consistently with <em>in situ</em> soil respiration. This finding is discussed in relation to previous measurements of soil C fluxes from the same system. In conclusion, we suggest that potential disturbances to the physical environment or the capacity of the decomposer community to facilitate litter decomposition are no longer clearly evident when clear-cut stands approach maturity.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"215 ","pages":"Article 106485"},"PeriodicalIF":5.0,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145155135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacterial communities and CN cycling drive microplastic-specific trade-off between greenhouse gas emissions and antibiotic resistance genes during reductive soil disinfestation","authors":"Yanlong Chen ,&nbsp;Fengyang Zhang ,&nbsp;Yuhan Zhang ,&nbsp;Risheng Xu ,&nbsp;Jingyi Mei ,&nbsp;Kun Wang ,&nbsp;Cui Li ,&nbsp;Fan Chen ,&nbsp;Yuheng Wang","doi":"10.1016/j.apsoil.2025.106487","DOIUrl":"10.1016/j.apsoil.2025.106487","url":null,"abstract":"<div><div>Microplastics coexist with various soil degradations in intensive agricultural systems. Reductive soil disinfestation (RSD), a microbial community-based strategy, is widely used to alleviate soil degradation. However, microplastics' impact on soil bacterial communities and C<img>N cycling during RSD remains unclear, with such changes potentially further affecting greenhouse gas emissions and the prevalence of antibiotic resistance genes (ARGs). A microcosm experiment was conducted using biodegradable poly(butylene adipate-<em>co</em>-terephthalate) (PB) and non-degradable polyethylene (PE), with six treatments: untreated control (CK), PB- or PE-amended soil (PB, PE), straw incorporation with flooding (RSD), and RSD combined with PB or PE (PBRSD, PERSD). Results showed that compared to CK, RSD increased CH₄ emissions by 12-fold without affecting CO₂, whereas PBRSD enhanced both by 20-fold and 29 %, respectively, while PERSD increased CH₄ by 6.1-fold. Though RSD elevated N₂O emissions by 89 % relative to CK, microplastics mitigated this increase by 29 % in PBRSD and 17 % in PERSD. RSD reduced soil ARG abundance from 0.18 % (CK) to 0.16 %, with PBRSD intensifying this reduction but PERSD counteracted it. Enrichment of key bacterial taxa (e.g., <em>Clostridium</em>, <em>Paenibacillus</em>) was closely linked to the coregulation of ARGs (multidrug, aminoglycoside) and C<img>N cycling processes (pyruvate metabolism, methanogenesis, denitrification). Collectively, under RSD, PB enhanced greenhouse gas emissions but reduced ARGs, whereas PE mitigated greenhouse gas emissions yet promoted ARG accumulation, driven by shifts in bacterial communities and C<img>N cycling. These findings reveal a bacterial community and C<img>N cycling driven microplastic-specific trade-off between greenhouse gas emissions and ARGs control under RSD, informing sustainable soil management.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"215 ","pages":"Article 106487"},"PeriodicalIF":5.0,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145155131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revealing the mechanism of Burkholderia sp. Pyr-1 driven removal of pyraclostrobin-contamination through soil microbial community function and assembly","authors":"Hongming Liu ,&nbsp;Siwen Wang ,&nbsp;Zhengqi Zhang ,&nbsp;Xiang Li ,&nbsp;Yujie Ren ,&nbsp;Yuanxiu Wang ,&nbsp;Junwei Huang ,&nbsp;Lina Sun","doi":"10.1016/j.apsoil.2025.106488","DOIUrl":"10.1016/j.apsoil.2025.106488","url":null,"abstract":"<div><div>Pyraclostrobin is a commonly used fungicide that pollutes the environment, and removing its residue has attracted widespread interest. However, the effect of pyraclostrobin on soil bacterial communities remain unknown. This study employed high-throughput sequencing to investigate the effects of high and low doses of pyraclostrobin, as well as its degrading strain <em>Burkholderia</em> sp. Pyr-1, on soil microbial communities. The results revealed that soil with bacterial concentrations exceeding 10⁸ CFU/g achieved a degradation percentage greater than 96 % within 14 days. Notably, strain Pyr-1 significantly altered the structure of soil microbial communities. Specifically, high concentrations of pyraclostrobin reduced phylogenetic diversity, whereas the introduction of strain Pyr-1 alleviated this effect. Over time, Pyr-1 drove the assembly of pyraclostrobin-associated strains within specific modules of the soil microbial network. Members of keystone taxa enabled the pyraclostrobin degradation in soil, and microbial community assembly in enriched modules predominantly driven by stochastic processes. This study provides novel insights into the functional and assembly effects of pyraclostrobin and its degrading strain Pyr-1 on soil microbial communities.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"215 ","pages":"Article 106488"},"PeriodicalIF":5.0,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145155214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rock powder type and soil texture shape microbial community shifts and plant biomass responses in highly weathered tropical soils","authors":"Rafael Lima Oliveira ,&nbsp;Nariane de Andrade ,&nbsp;Caio César Gomes Freitas ,&nbsp;Amanda Manuelly da Silva Oliveira ,&nbsp;Danilo Ferreira da Silva ,&nbsp;Mísia Souza Vieira ,&nbsp;Rafael Santana Mendonça ,&nbsp;Felipe Martins do Rêgo Barros ,&nbsp;Antonio Carlos de Azevedo ,&nbsp;Fernando Dini Andreote","doi":"10.1016/j.apsoil.2025.106489","DOIUrl":"10.1016/j.apsoil.2025.106489","url":null,"abstract":"<div><div>Silicate rock powders can enhance soil fertility and contribute to carbon sequestration, but their effectiveness depends on the rock type, soil characteristics, and microbial responses. While microbial shifts following rock powder application are reported in subtropical systems, little is known about these dynamics in highly weathered tropical soils with contrasting textures. To address this, we applied three rock powders (phonolite, diabase, and granite) at three doses (1.50, 3.00, and 5.00 Mg ha⁻¹) to sandy and clay tropical soils and evaluated their effects on <em>Urochloa brizantha</em> biomass, soil fertility, and microbial community composition. All rock powders increased plant biomass regardless of application rate, with 5.00 Mg ha⁻¹ significantly enhancing potassium uptake in sandy soil. Bacterial diversity was largely unaffected, whereas fungal communities responded strongly to treatments, indicating that fungi are sensitive indicators of rock powder application. LEfSe analysis revealed distinct microbial enrichments depending on both soil type and mineral amendment. In sandy soils, the application of diabase promoted specific bacterial genera such as <em>Subgroup_7</em>, <em>Actinospica</em>, <em>Ruminiclostridium</em>, and <em>Rhodomicrobium</em>. In contrast, granite amendment selectively enriched fungal taxa in both soil types, including <em>Chaetomium</em>, <em>Aspergillus</em>, <em>Pseudallescheria</em>, and <em>Conoideocrella</em>. These enriched taxa were correlated with plant biomass, potassium uptake, and soil chemical properties, suggesting functional roles in mineral weathering and nutrient mobilization. Notably, plant biomass responses were not directly linked to changes in soil fertility parameters, highlighting the importance of microbially mediated mechanisms. These findings emphasize the need to incorporate mineral-microbe interactions into tropical soil fertility and management frameworks.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"215 ","pages":"Article 106489"},"PeriodicalIF":5.0,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145155210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intercropping improves the complexity of cross-kingdom networks between bacterial and fungal communities associated with soil carbon cycling in Hainan island of China","authors":"Saisai Hou ,&nbsp;Chao Zu ,&nbsp;Longfei Liu ,&nbsp;Pengcheng Liu ,&nbsp;Shiyu Shang ,&nbsp;Ying Yuan ,&nbsp;Yuding Wang ,&nbsp;Can Wang ,&nbsp;Jie Kang ,&nbsp;Yaqi Zhao ,&nbsp;Liujie Hu ,&nbsp;Zhigang Li ,&nbsp;Wu Xiong ,&nbsp;Jianfeng Yang","doi":"10.1016/j.apsoil.2025.106455","DOIUrl":"10.1016/j.apsoil.2025.106455","url":null,"abstract":"<div><div>Black pepper (<em>Piper nigrum</em> L.) intercropped with areca palm (<em>Areca catechu</em> L.) represents the main composite planting pattern for black pepper production in Hainan Province, China. This intercropping system can enhance black pepper yield by altering soil physicochemical properties and microbial communities. However, the mechanisms by which microorganisms influence soil nutrient cycling and the changes in soil microbial communities—particularly cross-kingdom networks linking bacteria and fungi—remain largely unknown. Here, we comprehensively examined soil physicochemical properties, enzyme activities, microbial communities, and cross-kingdom networks of bacteria and fungi. Intercropping strengthened the relationships between soil nutrients and enzyme activities and promoted soil carbon cycling by increasing β-glucosidase activity, primarily driven by bacterial functions in carbon metabolism. Bacterial diversity responded more strongly to intercropping than fungal diversity. Moreover, this practice substantially increased the complexity of microbial co-occurrence networks in both bacterial and fungal communities. Intercropping also enhanced the interconnections between abiotic and biotic factors, particularly increasing the complexity and stability of cross-kingdom microbial networks.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"215 ","pages":"Article 106455"},"PeriodicalIF":5.0,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145109546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}