Applied Soil Ecology最新文献

{"title":"The different pathways of microbial regulation of organic carbon turnover in the topsoil and subsoil of coastal saline soil after long-term stubble return and subsoiling","authors":"Xunya Su ,&nbsp;Le Zhang ,&nbsp;Hao Meng ,&nbsp;Jiaxue Zhao ,&nbsp;Han Wang ,&nbsp;Lunxiao Shang ,&nbsp;Qiyuan Guo ,&nbsp;Yupeng Xing ,&nbsp;Xianliang Song ,&nbsp;Xuezhen Sun ,&nbsp;Xiaopei Zhang ,&nbsp;Xiaoli Tian ,&nbsp;Lili Mao","doi":"10.1016/j.apsoil.2025.105970","DOIUrl":"10.1016/j.apsoil.2025.105970","url":null,"abstract":"<div><div>Long-term stubble return and subsoiling increased the soil organic carbon (SOC) in coastal saline soil; however, the changes in SOC components and their regulatory role of microorganisms in this process remain unclear. Therefore, a 9-year experiment assessed stubble management (removal or return) and tillage (non-subsoiling or subsoiling) effects on SOC components and microbial communities to clarify microbial regulation of SOC turnover. Results showed that stubble return and subsoiling improved the physicochemical properties of the topsoil (0–20 cm) and subsoil (20–40 cm), and increased SOC and its components, including dissolved organic carbon (DOC) by 160 %, easily oxidizable carbon (EOC) by 59 %, microbial biomass carbon (MBC) by 171 %, particulate organic carbon (POC) by 103 %, and mineral-associated organic carbon (MOC) by 22 %. In the subsoil, stubble management significantly increased DOC, EOC, MBC, POC, and MOC by 21 %, 62 %, 27 %, 72 %, and 37 %, respectively. Through high-throughput sequencing and Biolog-ECO microplate, we found significant changes in the structure and metabolic function of microbial communities after stubble return and subsoiling. Notably, stubble return and subsoiling altered the microbial regulatory pathways in SOC turnover in different soil layers. In the topsoil, microorganisms primarily utilize EOC to convert it into POC, while the direct adsorption of DOC by minerals serves as the main pathway for MOC synthesis. In the subsoil, DOC serves as the primary carbon source for microorganisms, leading to the conversion to MBC and the promotion of MOC formation. Our results highlight that stubble return and subsoiling can optimize the microbial community structure and metabolic function and increase SOC sequestration with different regulation pathways in SOC component turnover, and the measures can be effective measures to improve the status of coastal saline soil.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"208 ","pages":"Article 105970"},"PeriodicalIF":4.8,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143511440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The growth of submerged plant Potamogeton crispus L. affected complete ammonia oxidizers in the rhizosphere","authors":"Jianzhao Li ,&nbsp;Ziqi Liu ,&nbsp;Yan Wang ,&nbsp;Naidong Xiao ,&nbsp;Xiaoqiong Wan ,&nbsp;Yumei Hua ,&nbsp;Jianwei Zhao","doi":"10.1016/j.apsoil.2025.105991","DOIUrl":"10.1016/j.apsoil.2025.105991","url":null,"abstract":"<div><div>Complete ammonia oxidizers (comammox bacteria) can independently convert NH₃ into NO₃⁻, which provides a new perspective to understand the cycling of nitrogen. This study investigated the effect of the growth of <em>Potamogeton crispus</em> L. (a submerged plant) on comammox bacteria in the rhizosphere sediment of the Tangxun Lake, Wuhan, China. The results showed that with the deceleration of <em>P. crispus</em> growth, the abundance of comammox clade A in the rhizosphere gradually increased, indicating that slower growth of <em>P. crispus</em> is conducive to the growth of comammox clade A in the rhizosphere. However, the growth of <em>P. crispus</em> seemed to have little impact on comammox clade B in the rhizosphere. Phylogenetic analysis revealed that clade A.1, clade A.2, and clade B of comammox bacteria accounted for 89.4 %, 0.7 %, 9.9 % of the total abundance, respectively, with clade A.1 showing overwhelming dominance. The nitrification rate of ammonia-oxidizing archaea (AOA), ammonia-oxidizing bacteria (AOB), and comammox bacteria in the rhizosphere was 0.18, 1.01, and 1.76 mg N kg⁻¹ d⁻¹, respectively, and comammox bacteria obviously played a dominant role in the nitrification process. Redundancy discriminant analysis demonstrated that NH₄⁺-N is the key environmental factor that affects the ammonia-oxidizing microorganisms in the rhizosphere sediments. The nitrification rate of comammox bacteria was significantly positively correlated with AOA and AOB. This study validated that the growth of plants can induce the differentiation of comammox bacteria in the rhizosphere sediment, which provides important clues for better understanding the microorganism-driven nitrogen transformation mechanisms.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"208 ","pages":"Article 105991"},"PeriodicalIF":4.8,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143511299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resource-enhancing global changes shift soil multifunctionality towards faster cycling in arid grasslands","authors":"Zhaobin Song ,&nbsp;Xiaoan Zuo ,&nbsp;Shaokun Wang ,&nbsp;Xiangyun Li ,&nbsp;Ya Hu ,&nbsp;Jingjuan Qiao ,&nbsp;Chao Wang ,&nbsp;Ellen L. Fry ,&nbsp;Jordi Sardans ,&nbsp;Josep Peñuelas ,&nbsp;Yann Hautier","doi":"10.1016/j.apsoil.2025.105987","DOIUrl":"10.1016/j.apsoil.2025.105987","url":null,"abstract":"<div><div>Soil multifunctionality in terrestrial ecosystems plays a pivotal role in providing sustainable services to humanity. Resource-enhancing global changes, such as increased precipitation and nitrogen (N) deposition can accelerate the transformation of various aspects of ecosystem functions from slow to fast cycling. The difference in how these global change factors influence soil multifunctionality in arid grasslands remains unknown, limiting our ability to manage these ecosystems under anthropogenic changes. Using a framework recently developed to quantify slow-to-fast cycling transitions in ecosystem functions, we tested the impact of increased precipitation and N addition on soil slow-fast multifunctionality and its components related to soil carbon (C), N, and phosphorous (P) functions by conducting two separate manipulative experiments in arid grasslands. Additionally, we explored the contribution of plant diversity, microbial diversity and soil properties to the variations of soil multifunctionality. We found that increased precipitation and N addition drove shifts in soil multifunctionality towards faster cycling. However, such shifts resulted from different responses of soil C, N and P functions. Specifically, increased precipitation resulted in faster C, N and P cycling functions, while N addition led to faster N cycling functions. Although above- and below-ground diversity and soil properties were closely linked to soil N and P functions, increased precipitation did not affect these abiotic and biotic drivers. Therefore, the impacts of increased precipitation on soil C, N and P functions were direct. In contrast, the impact of N addition on soil N functions was mediated through changes in above- and below-ground community composition and soil properties. Our results provide deeper insights into the driving mechanisms by which increased precipitation and N addition affect soil multifunctionality, indicating that the drivers of multifunctionality are context-dependent. Therefore, we should develop corresponding strategies to mitigate the impacts of different global change factors on soil multifunctionality in arid grasslands.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"208 ","pages":"Article 105987"},"PeriodicalIF":4.8,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143474779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mixed vineyard prunings and sheep manure compost: Creating changes in vineyard soil dominant microbiota to impact fruit quality","authors":"Qian Tu ,&nbsp;Yuanyuan Li ,&nbsp;Weikang Ding ,&nbsp;Shubo Zhou ,&nbsp;Yanping Huang ,&nbsp;Shiqiu Liu ,&nbsp;Xuwu Min ,&nbsp;Jiarui Zhang ,&nbsp;Junjun Li ,&nbsp;Chunlong Yuan","doi":"10.1016/j.apsoil.2025.105973","DOIUrl":"10.1016/j.apsoil.2025.105973","url":null,"abstract":"<div><div>Vineyard prunings are an excellent composting substrate when suitably treated. However, the potential link between vineyard prunings composting and promoting changes in soil microbial communities in vineyards and the impact on fruit quality remains unclear. This study aimed to assess the effects of various combinations of vineyard prunings and sheep manure compost on vineyard soil properties, microbial composition, and fruit quality. The results indicated that compost improved soil physicochemical properties. Changes in soil properties influenced microbial characteristics and functions, compost with a higher proportion of sheep manure altered the dominant bacterial phylum in the soil with <em>Actinobacteria</em> becoming predominant in 30 % vineyard prunings +70 % sheep manure composting (GM3) and 100 % sheep manure composting (SM), representing 30.7 % and 33.2 % of total abundance. The dominant bacterial genus in GM3 treatment was <em>SBR1031</em>, which increased its relative abundance by 6.9 %. These dominant microbiota were positively correlated with soil nutrients and enzyme activities, which promoted the release of available potassium (AK) and available phosphorus (AP). Microbial function prediction indicated that GM3 treatment upregulates metabolic pathways, including the citric acid cycle and amino acid metabolism. Moreover, soil characteristics demonstrated positive correlations with reducing sugars, soluble solids, and total tannins. Compost application, especially GM3, had a positive impact on fruit quality, outperforming pure sheep manure compost. The results highlight that compost from a combination of vineyard prunings and sheep manure fosters vineyard soil properties and microbial functions, thereby exerting a positive impact on Pinot Noir fruit.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"208 ","pages":"Article 105973"},"PeriodicalIF":4.8,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143480631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plant mycorrhizal type and molybdenum (Mo) contamination co-shape rhizospheric fungal communities in molybdenum mining areas","authors":"Shuang Yang ,&nbsp;Zhaoyong Shi ,&nbsp;Jiakai Gao ,&nbsp;Shouxia Xu ,&nbsp;Fayuan Wang","doi":"10.1016/j.apsoil.2025.105986","DOIUrl":"10.1016/j.apsoil.2025.105986","url":null,"abstract":"<div><div>Plants can selectively recruit rhizospheric microbiomes with specific ecological functions for their better survival, which are further shaped by environmental stress, such as soil contamination. However, little is known about the rhizospheric fungal communities in different mycorrhizal plants subjected to long-term molybdenum (Mo) contamination. For this purpose, we collected rhizospheric soil samples from the China's largest Mo mining area to explore how plant mycorrhizal type and Mo contamination level shape rhizospheric fungal communities. High-throughput sequencing results showed that the diversity of rhizospheric fungi was significantly higher in arbuscular mycorrhizal (AM) plants than in ectomycorrhizal (ECM) plants under heavy Mo contamination. The number of unique amplicon sequence variants (ASVs) in AM plants was greater than that of ECM plants, and this difference decreased with the increasing Mo level. Moreover, the potential biomarkers of rhizospheric fungi were differently enriched in AM and ECM plants under various Mo stress. FUNGuild functional prediction analysis indicated that the abundance of some ectomycorrhizal-associated fungi decreased with the increasing Mo level. The network complexity of rhizospheric fungi of ECM plants was greater than that of AM plants, whereas Mo contamination drove both networks more complex. Mo level mainly altered the composition of rhizospheric fungal communities in ECM plants, but not in AM plants. To conclude, plant mycorrhizal types and Mo contamination levels jointly shaped rhizospheric fungal communities in Mo mining areas. Our results indicate that AM plants may have evolved a survival strategy adaptive to long-term Mo contamination via recruiting rhizospheric fungi, and thus show great potential for ecological restoration in Mo mining areas.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"208 ","pages":"Article 105986"},"PeriodicalIF":4.8,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143465474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soil fauna in agroforestry contributes to the suppressiveness to plant-parasitic nematodes: A case study in a Mediterranean area","authors":"Anne-Sophie Masson ,&nbsp;Fanny Bouton ,&nbsp;Stéphane Bellafiore ,&nbsp;Jamel Aribi ,&nbsp;Claire Marsden ,&nbsp;Mickael Hedde ,&nbsp;Jean Trap","doi":"10.1016/j.apsoil.2025.105962","DOIUrl":"10.1016/j.apsoil.2025.105962","url":null,"abstract":"<div><div>Plant-parasitic nematodes are known to impair plant development and can cause severe crop loss. Agroforestry is a promising land use management system for the preservation of soil fertility and biodiversity conservation, but very few studies have focused on the regulation of plant-parasitic nematodes with the inclusion of tree alleys in cropping systems. In this study, the capacity of the soil to suppress plant-parasitic nematodes was assessed in Southern France. Fresh and heated soils from different combinations of land use (monospecific crop, agroforestry or tree plantation) and plant cover (crop or tree) were tested for their capacity to suppress <em>Meloidogyne javanica</em> in a laboratory assay. In the samples taken under tree cover, the suppressive capacity of fresh soils was improved compared to that of the monospecific crop samples. As the suppressive capacity of the heated soils remained low, we considered that soil fauna was responsible for part of the soil suppressiveness. The characterization of nematode communities revealed no <em>Meloidogyne</em> spp. on the experimental site, but other plant-parasitic nematodes were found. The total density of <em>Pratylenchus</em> spp. was lower while the relative density of <em>Helicotylenchus</em> spp. was higher under tree cover, compared with the monospecific crop soils. In agroforestry, the relative density of herbivores was ca 200 % higher under tree cover compared to under crop cover but the parasitic pressure (assessed by the Plant-Parasitic Index/Maturity Index ratio) was ca 30 % lower. Moreover, the crop soils had the highest Enrichment Index while the tree row soils in agroforestry had the highest Structural Index. The suppressive capacity in agroforestry was associated with a specific nematofauna, including more predatory taxa than in the monospecific crop. This study showed that including trees in a cropping system in a Mediterranean area created a favorable niche for potential herbivore regulators among the soil fauna. Further investigations are required to validate the regulation of plant-parasitic nematodes, and to disentangle the complex interactions explaining it in agroforestry.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"208 ","pages":"Article 105962"},"PeriodicalIF":4.8,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143453004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of recycled organic waste amendments on carbon pools, greenhouse gas emissions, and nematode indicators of soil health","authors":"J. Milkereit ,&nbsp;M. Burger ,&nbsp;A.K. Hodson","doi":"10.1016/j.apsoil.2025.105967","DOIUrl":"10.1016/j.apsoil.2025.105967","url":null,"abstract":"<div><div>To optimize outcomes for plant and soil health, greater understanding is needed about how management practices affect interrelationships between soil organic carbon and soil food webs, which has important implications for greenhouse gas emissions. This study examined the effects of applying three different organic amendments, from sources previously considered waste. The amendments included: 1) compost composed of municipal food waste and yard trimmings 2) biochar produced from woody debris and 3) liquid food hydrolysate derived from grocery store organics. These amendments were compared to either fertilized (N+) or unfertilized (N-) controls. In an almond orchard field trial, both biochar and compost increased pools of total carbon and large macroaggregates compared to N+ and N- controls, but only compost increased the carbon pool, permanganate oxidizable carbon (POXC). Average daily nitrous oxide (N₂O) emissions during the first year were significantly higher in N+ and food hydrolysate than in biochar, compost and N- treatments, likely due to their lower C:N ratio. Biochar decreased the total number of nematodes and root herbivores compared to N- controls, while compost, food hydrolysate and N+ treatments increased the abundance of root-herbivores. Food hydrolysate additionally increased the ratio of key fungal-feeding/bacterial-feeding nematodes compared to N+ treatments. Fertilizer treatments increased tree growth while both food hydrolysate and fertilizer increased leaf nitrogen and potassium. The incubation study showed that food hydrolysate released more net mineralized inorganic N than other amendments or N+ treatments and that compost consistently increased microbial biomass carbon. These results may be used to optimize the selection of organic amendments and indicate that there are benefits and tradeoffs in terms of their effects on carbon storage, soil biology and greenhouse gas emissions.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"208 ","pages":"Article 105967"},"PeriodicalIF":4.8,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143453025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of nitrogen fertilizer replacement with different sourced-black soldier fly frass on rice growth, physiological characteristics and soil properties","authors":"Yajing Cao ,&nbsp;Nan Wu ,&nbsp;Lijun Shi ,&nbsp;Xiaoyan Xu ,&nbsp;Xiaobo Wang","doi":"10.1016/j.apsoil.2025.105975","DOIUrl":"10.1016/j.apsoil.2025.105975","url":null,"abstract":"<div><div>Application of black soldier fly larvae (BSFL) frass had significant effects on crop yield and soil quality. However, the effects of different sourced-frass on crops and soil had been largely unknown. Here, the effects of partial replacement (30 %) of nitrogen fertilizer with different BSFL frass sourced from pig manure (PBF), chicken manure (CBF), and kitchen waste (KBF) on soil properties, rice growth, and physiological functions were investigated. Control groups without nitrogen fertilizer (CK) and with pure chemical fertilizer (CF) were included. Compared with CF, all insect frass treatments increased soil pH. PBF treatment increased soil available nitrogen by 7.68 %, and both PBF and CBF increased available potassium contents by 8.50 %–8.54 %. Applying insect frass did not significantly affect the diversity and structure of soil microbial communities. Compared to CF, PBF enriched carbon-metabolizing phyla such as <em>Chloroflexi</em> (10.35 %) and eutrophic phyla like <em>Actinobacteriota</em> (27.45 %), as well as enriched soil families such as <em>Gemmatimonadaceae</em> and <em>Geminicoccaceae</em>. PBF improved the physiological characteristics in rice compared to CF and increased the photosynthetic rate (21.42 %) and sucrose synthase activity (32.00 %) of rice leaves at the booting stage. Moreover, the seed yield of PBF-treated rice showed a 7.94 % increase over CF, but KBF exhibited significantly lower seed yield compared to CF. In summary, the use of BSFL frass derived from pig manure as a partial substitute for nitrogen fertilizers had the most favorable impact on rice growth and physiological functions, surpassing the effects of purely chemical fertilizers.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"208 ","pages":"Article 105975"},"PeriodicalIF":4.8,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143465475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Loss of protistan diversity weakens soil phosphorus availability","authors":"Zhechang Mei ,&nbsp;Chuanfa Wu ,&nbsp;Shengjing Shi ,&nbsp;Haoqing Zhang ,&nbsp;Zhenke Zhu ,&nbsp;Jianping Chen ,&nbsp;Tida Ge","doi":"10.1016/j.apsoil.2025.105976","DOIUrl":"10.1016/j.apsoil.2025.105976","url":null,"abstract":"<div><div>Plants and their associated microbiomes symbiotically regulate the phosphorus(P) availability in rhizosphere soils. However, the impact of protists, a regulator of other microbes (bacteria and fungi) in the soil food webs, on soil P availability is not well-known. we simulate biodiversity loss using dilution-to-extinction method and explore its effects on P availability through a greenhouse experiment. Additionally, we investigate the implications for soil P availability. We noted a substantial reduction in protistan diversity in the diluted treatment relative to the undiluted one, with a peak decrease of 74.5 %. The protistan alpha diversity showed a significant positive correlation with available phosphorus (AP) content and phosphatase (PHOS) activity, suggesting that protistan diversity influences P availability. Additionally, Linear regression analysis revealed that the size and stability of the protistan co-occurrence network were significantly positively correlated with soil AP and PHOS, indicating that the reduction in protistan interactions weakened soil P availability under wheat cultivation. Structural equation model and random forest modeling analysis indicated that protistan network interactions and diversity are the major predictors of AP content and PHOS activity. Dilution modulated the relationship between protistan community and soil P availability. Our findings suggest that the protistan community plays a crucial role in maintaining soil P availability, particularly in monoculture agricultural systems. The study highlights the importance of considering the impacts of protistan diversity loss on ecosystem functions, especially in the context of intensive agricultural practices and climate change.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"208 ","pages":"Article 105976"},"PeriodicalIF":4.8,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143465473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Litter removal and nitrogen deposition alter soil microbial community composition and diversity in a typical rubber (Hevea brasiliensis) plantation of Hainan, China","authors":"Xinxin Xue ,&nbsp;Changqi Ren ,&nbsp;Chunmei Zhao ,&nbsp;Wenbin Wang ,&nbsp;Xuehua Luo ,&nbsp;Yongfa Zhang ,&nbsp;Zhengzao Cha","doi":"10.1016/j.apsoil.2025.105969","DOIUrl":"10.1016/j.apsoil.2025.105969","url":null,"abstract":"<div><div>Litter manipulation and nitrogen (N) deposition modify the soil microbial communities owing to the modifications in carbon input and soil nutrient availability. However, the mechanism behind the effects of litter removal (LR), N deposition, and their interactions on the diversity, richness and composition of soil microbial communities in tropical rubber (<em>Hevea brasiliensis</em>) plantation of China remains to be studied. To fill this research gap, we established a simulation field experiment to remove the rubber litter under the different anthropogenic inputs of N in Hainan island in 2019. The experiment included eight treatments: no N deposition (CK), low-N deposition (LN, 5 g N m⁻² yr⁻¹), medium-N deposition (MN, 10 g N m⁻² yr⁻¹) and high-N deposition (HN, 20 g N m⁻² yr⁻¹) with the LR and litter left intact (LI), respectively. Soil samples were collected from the top 0–10 cm layer, and the high-throughput sequencing was conducted to quantify the soil community structure and diversity of bacteria and fungi. Overall, HN treatment considerably diminished the bacterial Shannon index by 7.3 % but elevated the fungal Chao1 index by 18.6 % in the LI soil. Moreover, LR markedly decreased bacterial Chao1 index by 4.2 %, Shannon index by 6.3 %, and fungal Chao1 index by 20.0 % under MN treatment and those of fungi under HN deposition. Structural equation model indicated that increased NO₃⁻-N concentration reduced bacterial Shannon index driven by LR and elevated N deposition, while the LR boosted the activity of cellulase, which resulted in a decrease in fungal Chao1 index. PCoA analysis indicated that N deposition altered the bacterial communities more substantially than fungal communities, whereas LR led to distinct shifts in the bacterial and fungal communities. Furthermore, redundancy analysis indicated that alterations in soil NO₃⁻-N and light fraction organic carbon (LFOC) were the primary factors influencing soil bacterial and fungal community compositions, respectively, which were driven by N deposition and litter manipulation. Conclusively, N deposition strengthened the adverse impact of litter removal on the soil microbial community, which in turn adversely affects the sustainable development of rubber forest ecosystems. These findings suggested that maintaining adequate litter cover and N inputs is essential for rubber plantation management, as it has a positive synergistic effect on the diversity and composition of soil microbial communities.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"208 ","pages":"Article 105969"},"PeriodicalIF":4.8,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143453003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}