Revealing the mechanism of Burkholderia sp. Pyr-1 driven removal of pyraclostrobin-contamination through soil microbial community function and assembly

Abstract

Pyraclostrobin is a commonly used fungicide that pollutes the environment, and removing its residue has attracted widespread interest. However, the effect of pyraclostrobin on soil bacterial communities remain unknown. This study employed high-throughput sequencing to investigate the effects of high and low doses of pyraclostrobin, as well as its degrading strain Burkholderia sp. Pyr-1, on soil microbial communities. The results revealed that soil with bacterial concentrations exceeding 10⁸ CFU/g achieved a degradation percentage greater than 96 % within 14 days. Notably, strain Pyr-1 significantly altered the structure of soil microbial communities. Specifically, high concentrations of pyraclostrobin reduced phylogenetic diversity, whereas the introduction of strain Pyr-1 alleviated this effect. Over time, Pyr-1 drove the assembly of pyraclostrobin-associated strains within specific modules of the soil microbial network. Members of keystone taxa enabled the pyraclostrobin degradation in soil, and microbial community assembly in enriched modules predominantly driven by stochastic processes. This study provides novel insights into the functional and assembly effects of pyraclostrobin and its degrading strain Pyr-1 on soil microbial communities.