Applied Soil Ecology最新文献

{"title":"Symbiotrophs dominate rhizosphere soil fungal community stability of the Cathaya argyrophylla","authors":"Zhibo Zhou,&nbsp;Peng Xie,&nbsp;Kerui Huang,&nbsp;Ping Mo,&nbsp;Aihua Deng,&nbsp;Shaogang Fan,&nbsp;Binbin Long,&nbsp;Yun Wang","doi":"10.1016/j.apsoil.2024.105719","DOIUrl":"10.1016/j.apsoil.2024.105719","url":null,"abstract":"<div><div><em>Cathaya argyrophylla</em> is an ancient and endangered pine tree unique to China and is classified as an endangered species due to climate deterioration and glacier action, etc. However, there is limited research of its rhizosphere microbiome and this information is essential for potential modifications of its habitat to allow for its continued survival. The current study collected rhizosphere soil from the two remaining <em>C. argyrophylla</em> nature reserves in Hunan Province and explored the changes in community characteristics of rhizosphere soil fungal functional groups (symbiotrophs, saprotrophs and pathotrophs). We evaluated their impact on fungal community stability using the average variation degree (AVD). The soil fungal community stability in the Chengbu area was significantly higher than that in the Chenzhou area (F = 6.34, <em>P</em> &lt; 0.05) and this was closely related to the significant differences in α- (F_Shannon = 14.85, F_Chao1 = 12.97, <em>P</em> &lt; 0.01) and β-diversity of symbiotic fungal communities. We also found that the network stability (r = 0.741, <em>P</em> &lt; 0.01) and niche breadth (r = 0.598, <em>P</em> &lt; 0.05) for symbiotrophs were significantly correlated with AVD. Additionally, <em>Russula</em> and <em>Mortierella</em> were core symbiotrophs shared in the rhizosphere soil of <em>C. argyrophylla</em>. Overall, we found that symbiotrophs were particularly important for soil fungal community stability in <em>C. argyrophylla</em> habitats and identified core taxa involved in these processes. This work provides a reference basis for policies and management measures to protect the ecosystem services of the <em>C. argyrophylla</em> community from the perspective of the soil microbiome.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"204 ","pages":"Article 105719"},"PeriodicalIF":4.8,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142553217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential effects of fresh and composted organic wastes on soil bacterial community and maize growth","authors":"Guilherme Lucio Martins ,&nbsp;Thierry Alexandre Pellegrinetti ,&nbsp;Anderson Santos de Freitas ,&nbsp;Gabriel Silvestre Rocha ,&nbsp;Gabriel Gustavo Tavares Nunes Monteiro ,&nbsp;Lucas William Mendes ,&nbsp;Siu Mui Tsai ,&nbsp;Fernando Carvalho Oliveira ,&nbsp;Jussara Borges Regitano","doi":"10.1016/j.apsoil.2024.105715","DOIUrl":"10.1016/j.apsoil.2024.105715","url":null,"abstract":"<div><div>The use of organic waste in agriculture improves soil health although its interaction with mineral fertilizers is not fully understood. This study investigated the impact of combining mineral fertilizers with organic wastes, such as sugarcane filter cake, poultry litter, and chicken manure in both fresh and composted forms, on maize (<em>Zea mays</em>) growth, soil bacterial communities, and their potential metabolic functions. The assessment was conducted 90 days post-application on two different soil types (<em>i.e.</em>, clay and sandy clay loam) in a greenhouse setting. While fresh animal-derived organic wastes generally boost short-term plant growth and nutrient uptake, composted wastes can be more effective in reducing nitrogen losses and methane emissions over the long term. The impact of organic waste on soil microbial communities and functions varies, with fresh wastes increasing nitrification and methanogenesis, while composted wastes tend to stabilize carbon and reduce these processes. Evaluating different organic waste sources and forms, and considering their C:N ratios, is crucial for optimizing soil health and minimizing greenhouse gas emissions. They also boosted plant growth and nutrient uptake, whereas composted waste increased soil mineral-associated organic matter (a more stable carbon pool) and phosphorus availability. These findings are crucial for optimizing the synergy between organic wastes and mineral fertilizers to enhance soil productivity and agricultural sustainability.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"204 ","pages":"Article 105715"},"PeriodicalIF":4.8,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142553214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Arbuscular mycorrhizal fungi promote soil ammonia but not nitrate assimilation in the Mu Us Desert","authors":"Guannan Zhu ,&nbsp;Yangui Qiao ,&nbsp;Chun Miao ,&nbsp;Lin Miao ,&nbsp;Shugao Qin ,&nbsp;Yuqing Zhang","doi":"10.1016/j.apsoil.2024.105726","DOIUrl":"10.1016/j.apsoil.2024.105726","url":null,"abstract":"<div><div>Although arbuscular mycorrhizal fungi (AMF) have been established to assimilate nitrogen (N) and transport this to host plants, the potential role of AMF in soil N processes, particularly the assimilation of inorganic N, has not been comprehensively investigated. Compared with other terrestrial ecosystems, deserts are typically nitrogen-poor environments. Numerous species of xerophytic shrubs growing in deserts, such as <em>Artemisia ordosica</em>, form efficient symbiotic relationship with AMF, and play an important role in ecological restoration in China. However, whether arbuscular mycorrhizal symbioses are advantageous with respect to the competition for N resources and inhibition of N assimilation by other soil microbes in nitrogen-poor soils has yet to be established. To gain further insights in this regard, we established a culture system of the xerophyte <em>A. ordosica</em> and AMF, and investigated the effects of AMF on soil nitrate reductase (NR) and nitrite reductase (NiR) activities, the abundance of genes involved in N processes, and soil microbial composition based on controlled experiments designed to elucidate the roles of AMF in soil inorganic N assimilation. We accordingly found that AMF enhanced the assimilation of soil NH₄⁺-N and NO₂⁻-N by promoting soil NiR activity, upregulating the abundance of <em>gltB</em> and <em>gltD</em> genes, and influencing the soil microbial community. However, AMF were found to inhibit soil NR activity and showed no promotive effect with respect the assimilation of NO₃⁻-N in the soil. Although compared with natural conditions, our experiments simplified the structure of the soil microbial community and may have amplified AMF function, on the basis of our findings, we identified a new N-utilization mechanism of AMF and <em>A. ordosica</em> symbionts. Specifically, we found that the fungal partners promote soil NH₄⁺-N and NO₂⁻-N assimilation, but not that of NO₃⁻-N, while also promoting the growth of <em>A. ordosica</em>. This can reduce the likelihood of soil NO₃⁻-N being consumed by other soil microbes. The findings of this study will contribute to enhancing our understanding of the survival of desert plants and AMF in nitrogen-deficient environments.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"204 ","pages":"Article 105726"},"PeriodicalIF":4.8,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142553216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustaining vulnerable agroecosystems with compost: Lasting benefits to soil health and carbon storage in semiarid winter wheat (Triticum aestivum, L.)","authors":"Hannah Rodgers ,&nbsp;Jay Norton ,&nbsp;Urszula Norton ,&nbsp;Linda T.A. van Diepen","doi":"10.1016/j.apsoil.2024.105716","DOIUrl":"10.1016/j.apsoil.2024.105716","url":null,"abstract":"<div><div>Wheat agroecosystems provide one of the most important crops worldwide yet are often threatened by land degradation and climate change, making it vital to find practices that sustain soil health. Organic amendments can regenerate degraded soils, provide plant-available nutrients, and sequester carbon (C), and have long-lasting impacts in semiarid regions. This project evaluated the legacy of compost (0–50 Mg ha⁻¹) in an organic winter wheat-fallow rotation by comparing soil health and plant growth either 1–2 or 5–6 years after compost application, with or without cover crops. We assessed soil chemistry, structure, labile organic matter pools, microbial activity (by extracellular enzymes), and microbial community composition (by phospholipid fatty acids). We aimed to understand how compost application and cover cropping contribute to long-term soil health and C storage in semiarid regions, and how these practices impact soil microbiology. Compost resulted in sustained increases in organic matter pools, but only marginally benefitted plant growth and soil structure. Compost did not impact microbial community composition or activity, possibly because cold and dry conditions inhibited microbial functioning and compost decomposition. Cover crops did not impact soil organic matter or microbiology. Overall, we found that compost can contribute to long-term soil C storage in semiarid degraded soils such as these, despite having no measurable impacts on soil microbial biomass or activity.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"204 ","pages":"Article 105716"},"PeriodicalIF":4.8,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142537398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vaccinium corymbosum interact with mycorrhizal fungi to affect nitrogen metabolism and alleviate soil nutrient limitation","authors":"Jing Jiang ,&nbsp;Zhiyu Yang ,&nbsp;Chunyu Liu ,&nbsp;Haifeng Zhu ,&nbsp;Huizhi Zhang ,&nbsp;Hongyi Yang ,&nbsp;Lili Li","doi":"10.1016/j.apsoil.2024.105713","DOIUrl":"10.1016/j.apsoil.2024.105713","url":null,"abstract":"<div><div>By helping their hosts access soil nitrogen (N), ericoid mycorrhizal fungi (EMF) perform a crucial role in ecosystem processes. However, how plants sense and take up N during EMF symbiosis and the integration of EMF in the soil N cycle with the analysis of N balance and symbiotic dynamics is still far from being well understood. In this study, the experimental system of EMF <em>Oidiodendron maius</em> 143 inoculated and non-inoculated blueberry plants under three N levels were established. Results showed that EMF inoculation significantly improved plants soluble protein content, chlorophyll content, total N, NO₃⁻ and NH₄⁺ contents, and mediated changed in root configuration under low N treatments. EMF-inoculated plants significantly increased the activities of N-related enzymes and up-regulated N metabolism genes. However, as the N level rose, these effects eventually disappeared and the rate of EMF colonization significantly decreased. Congruently, EMF inoculation also significantly alleviated microbial N limitation under low N conditions. EMF inoculation and soil organic carbon were the main causes of microbial P limitation by influencing soil available P, and N levels directly regulated P limitation. Microbial C limitation was mainly mediated by N levels, pH and soil N:P ratios, where N levels mediate soil acidification. In conclusion, our results suggested that low N levels favored blueberry symbiosis with EMF, and N levels influenced C, P, and N limitation of soil microbial communities, where mycorrhizal fungi may play an important role in balancing nutrient stoichiometry.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"204 ","pages":"Article 105713"},"PeriodicalIF":4.8,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142553293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soil bacterial community composition rather than diversity exhibits edge effects in a farming-pastoral ecotone","authors":"Aiai Xu ,&nbsp;Jie Liu ,&nbsp;Xiangzhou Zheng ,&nbsp;Yushu Zhang ,&nbsp;Xianzhang Pan","doi":"10.1016/j.apsoil.2024.105722","DOIUrl":"10.1016/j.apsoil.2024.105722","url":null,"abstract":"<div><div>Edge effects have significant implications for ecological conservation and biodiversity maintenance. However, whether soil bacteria exhibit edge effects in large-scale ecotones remains unclear. In this study, we investigated soil bacterial communities in the farming-pastoral ecotone of northern China (FPEN), one of the world's largest ecotones, and compared them with those in adjacent grassland and cropland areas. Paired samples from both land types within the mosaic distribution of the ecotone revealed that land uses exerted relatively minor effects on soil bacterial communities in the FPEN. Across all samples, soil bacterial diversity, including Shannon and Faith's PD, followed a trend of grassland &lt; ecotone &lt; cropland. Interestingly, the entire ecotone acted as a semipermeable barrier, blocking 10.1 % of OTUs from moving from grassland to cropland and 9.3 % from cropland to grassland, while 3.6 % of OTUs were confined to the ecotone as endemic taxa. This led to the highest OTU richness in the FPEN. Compared to the adjacent ecosystems, the soil bacterial community composition in the ecotone showed significant differences and the lowest similarity. These results underscore the importance of identifying and preserving the intact FPEN to maintain soil bacterial diversity. Furthermore, the genera <em>Ferruginibacter</em> and <em>Opitutus</em> and the families <em>Sporichthyaceae</em> and <em>P3OB-42</em> were identified as biomarkers of the ecotone, providing essential references for its accurate localization. In addition, the community similarity decreased with increasing environmental distance across all three ecosystems but was consistently lowest within the ecotone, indicating its susceptibility to environmental changes. Climate and vegetation conditions, particularly the normalized difference vegetation index (NDVI) and mean annual wind speed (MAWS), were the primary drivers of changes in soil bacterial diversity and community composition. In summary, soil bacterial community composition rather than diversity exhibited edge effects in the FPEN.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"204 ","pages":"Article 105722"},"PeriodicalIF":4.8,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142553210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Land use intensity differently affects soil microbial functional communities in arable fields","authors":"Chenguang Gao ,&nbsp;S. Emilia Hannula ,&nbsp;Peter M. van Bodegom ,&nbsp;T. Martijn Bezemer ,&nbsp;Franciska T. de Vries ,&nbsp;Jan Hassink ,&nbsp;Michiel H. in ’t Zandt ,&nbsp;Gabriel Y.K. Moinet","doi":"10.1016/j.apsoil.2024.105723","DOIUrl":"10.1016/j.apsoil.2024.105723","url":null,"abstract":"<div><div>Land use intensification can influence soil microbial communities and their functional potential. However, the impacts of different aspects of land use intensification on functional groups of soil microbes remain insufficiently elucidated in agroecosystems. This study investigated soil microbial groups and their functional potential in arable fields embedded in a gradient of land use intensity (LUI), integrating multiple agricultural practices in the Netherlands. The results reveal changes in soil bacterial and fungal functional groups along the LUI gradient, with a strong negative relationship between LUI and the diversity of many fungal functional groups. Changes in LUI also led to significant changes in the composition of both bacterial and fungal functional communities. Specifically, irrigation and pest control were identified as the primary practices influencing the community assembly of soil microbial functional groups. We further show that the connectivity between soil fungal functional groups decreased under higher LUI. In summary, our findings demonstrate that reducing land use intensity may have positive impacts on the functional potential of soil microbial communities, particularly for soil fungi. Sustainable management practices particularly related to irrigation and pest control may alleviate some of the observed adverse effects.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"204 ","pages":"Article 105723"},"PeriodicalIF":4.8,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142553294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Does increased cropping intensity translate into better soil health in dryland wheat systems?","authors":"Shikha Singh ,&nbsp;Surendra Singh ,&nbsp;William F. Schillinger ,&nbsp;Haly Neely ,&nbsp;Shannon B. Cappellazzi ,&nbsp;Charlotte Norris","doi":"10.1016/j.apsoil.2024.105728","DOIUrl":"10.1016/j.apsoil.2024.105728","url":null,"abstract":"<div><div>Despite reliable grain yields, the 13-month fallow period in winter wheat (<em>Triticum aestivum</em> L.) - fallow systems (WF) in low precipitation zones (&lt;350 mm annually) poses several challenges including soil erosion, soil organic matter (SOM) decline, weed management, and overall soil health degradation. Therefore, considerable research efforts are focused on reducing the frequency of fallow and reducing or eliminating tillage. We compared the widely practiced 2-year WF rotation to two 3-year crop rotations with only one year of fallow and continuous annual spring cropping. These systems have been evaluated over the past 10-plus years using undercutter conservation-till and no-till practices in a large-scale and long-term cropping systems experiment near Ritzville, WA USA. To date there is limited research-based information about the influence of these intensified crop rotations using conservation- and no-till compared to traditional conservation-till WF on soil health indicators, particularly microbial activity, and microbial community dynamics in the dryland region of inland Pacific Northwest (iPNW). This study was conducted as a part of Soil Health Institute's North American Project to Evaluate Soil Health Measurements (NAPESHM). We leveraged the aforementioned ongoing long-term (25-year-old) experiment to obtain these data. Soil samples were collected at 0–15 cm depth from different crop rotation treatments that included, 1. winter triticale (<em>X Triticosecale Wittmack</em>)-spring wheat-no till fallow (WT-SW-NTF); 2. continuous annual no-till spring wheat (CSW-NT); 3. winter wheat-undercutter conservation-till fallow (WW-TF); and 4. winter wheat-spring wheat-undercutter conservation-till fallow (WW-SW-TF). Soil samples were analyzed for an array of soil health indicators. Out of the 21 tested soil health indicators, eleven indicators were significantly influenced by crop rotations. These were SOM, carbon mineralization potential (24-h respiration), water extractable carbon (WEC), active C, potentially mineralizable nitrogen (PMN), water extractable nitrogen (WEN), soil pH, NO₃-N and NH₄-N, phosphatase enzyme activity, and wet aggregate stability (WAS). Within responsive indicators, all C-related biological indicators (SOM, WEC, active C, and 24-h respiration) were highest under WW-SW-TF rotation while N-related indicators (WEN, PMN, and inorganic N) were highest under CSW-NT rotation. The improvements in aforementioned soil properties in the 3-year winter wheat-spring wheat-undercutter conservation-till fallow rotation was likely because of the higher crop biomass production in this rotation as compared to the other tested rotations.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"204 ","pages":"Article 105728"},"PeriodicalIF":4.8,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142553213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stability of nitrogen-cycling microbial communities and impact on microbial nitrogen function under different land use practices","authors":"Yongpeng Zhao ,&nbsp;Zhihui Wang ,&nbsp;Kai Cai ,&nbsp;Shuling Wang ,&nbsp;Alan L. Wright ,&nbsp;Xianjun Jiang","doi":"10.1016/j.apsoil.2024.105729","DOIUrl":"10.1016/j.apsoil.2024.105729","url":null,"abstract":"<div><div>Land use management practices can strongly influence microbial community composition. However, it remains unclear whether land uses affect community stability, especially for the entire nitrogen (N) transforming community whose stability is the basis of soil microbiome N-functioning. Using metagenomic sequencing, this study investigated the co-occurrence patterns of N-cycling microbes in forest, upland, and paddy soils and their relationship with N-functional genes. The co-occurrence network structures of soil N-cycling community for these three typical land uses were completely different, with the most complex interactions observed in paddy soils (e.g. more nodes and edges, and higher average clustering coefficient, average degree, and relative modularity). The N-cyclers stability (robustness and negative: positive cohesion) in paddies was significantly higher than in uplands and forests, and lowest in forests. A random forest model revealed that soil moisture and available N emerged as the primary predictors of N-cyclers stability, with stability increasing as these factors increased. Moreover, community stability of soil N-cyclers was strongly associated with N functions. Specifically, N-cyclers stability was positively linked to multiple dissimilatory N-cycling genes and negatively correlated with several N assimilatory genes. In conclusion, our results demonstrate that land use practices significantly affect the stability of N-cycling microbial communities, which in turn influences microbial N-functionality.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"204 ","pages":"Article 105729"},"PeriodicalIF":4.8,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142553215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Arenosol Epieuric and Haplic Cambisol show a similar level of resilience of microbial communities when irrigated with treated wastewater in a temperate climate","authors":"Alica Chroňáková ,&nbsp;Michal Choma ,&nbsp;Lucie Kotrbová ,&nbsp;Ana Catalina Lara ,&nbsp;Clara Villeneuve ,&nbsp;Rosa Paulina Calvillo-Medina ,&nbsp;Veronika Jílková ,&nbsp;Radka Kodešová","doi":"10.1016/j.apsoil.2024.105693","DOIUrl":"10.1016/j.apsoil.2024.105693","url":null,"abstract":"<div><div>The reuse of treated wastewater for irrigation purposes in agriculture is a common but controversial means of saving freshwater and providing plants with nutrients. It often leads to a change in physico-chemical properties and the introduction of uncontrolled amounts of pollutants into the soil. As a result, the structures and functions of soil microbial communities can change to an unknown extent. The aim of this study was to investigate the effect of treated wastewater on the abundance, diversity, and composition of bacteria, fungi, and ammonia oxidizers in two arable soils, the Arenosol and Cambisol. The raised soil beds were regularly irrigated with tap water (W) or effluent (E) and used for a vegetable crop rotation. Changes in soil chemical properties, nutrient content, abundance and composition of ammonia oxidizers, bacteria, and fungi were evaluated after 0, 54, 115, and 152 days. Irrigation with effluent led to an increase in salinity in both soils, while dissolved organic carbon (C) content, nitrate concentration and oxidizable C content showed soil-dependent response. Neither soil bacteria nor ammonia oxidizers were affected by the effluent in any soil, but time-dependent differences between fungal communities between W- and E-irrigated soils were observed. Our results indicate microbial taxa or guilds that were more sensitive to wastewater irrigation (<em>Apiotrichum</em>) and those that can thrive in altered soil conditions (<em>Plectosphaera</em> and <em>Pseudopithomyces</em>). However, this effect was overshadowed by changes caused by the crop rotation, indicating high quality of treated wastewater used for irrigation.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"204 ","pages":"Article 105693"},"PeriodicalIF":4.8,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142535206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}