Applied Soil Ecology最新文献

{"title":"Impact of bacterial inoculations on Pisum sativum L. biometric parameters and associated bacterial and AM fungal communities under semi-arid field conditions in Tunisia","authors":"Houda Ilahi ,&nbsp;Alice Calvo ,&nbsp;Sana Dhane ,&nbsp;Mustapha Missbah El Idrissi ,&nbsp;Lahcen Ouahmane ,&nbsp;Mohamed Najib Alfeddy ,&nbsp;Thomas Reitz ,&nbsp;Fabiano Sillo ,&nbsp;Raffaella Balestrini ,&nbsp;Bacem Mnasri","doi":"10.1016/j.apsoil.2024.105757","DOIUrl":"10.1016/j.apsoil.2024.105757","url":null,"abstract":"<div><div>In Mediterranean agroecosystems, pea (<em>Pisum sativum</em> L.) is one important crop due to its nutritional benefits and high protein content. However, soil nutrient availability and soil health are known to affect pea productivity, especially under arid and semi-arid conditions. Currently, the use of plant growth-promoting bacteria (PGPB) may represent a bio-based tool to improve pea productivity in drought-affected areas. Nevertheless, there is limited knowledge on how PGPB inoculations in field could impact native communities of bacteria and arbuscular mycorrhizal fungi (AMF) in these areas. Here, a two-year field study in Tunisia was established to evaluate the effects of inoculating two pea varieties with three strains of potential PGPB, including <em>Rhizobium laguerreae</em> and two strains of <em>Erwinia</em> sp., on agronomic performance and soil microbial communities. Inoculations improved productivity and all measured biometric parameters, with the treatment including a consortia of the three strains showing the highest benefits. Metabarcoding analysis showed an increased bacterial and AM fungal diversity in soil of inoculated plants. Additionally, specific AMF-bacterial associations were identified, suggesting a synergistic role in enhancing soil health and pea growth. Overall, this study highlights the potential of targeted bacterial inoculations to improve pea performance under semi-arid environments by exploiting beneficial plant-microbe interactions. These results support the use of microbial inoculants as a sustainable agricultural practice in semi-arid areas, also improving the understanding of their impact on native bacterial and AM fungal communities.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"205 ","pages":"Article 105757"},"PeriodicalIF":4.8,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142702180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distance-dependent, species-specific effects of legumes on nitrogen utilization by companion non-legumes in the Gurbantunggut desert, Northern China","authors":"Yuxin Xiao ,&nbsp;Xinyu Zhang ,&nbsp;Boyi Song ,&nbsp;Nargiza Galip ,&nbsp;Weiwei Zhuang","doi":"10.1016/j.apsoil.2024.105765","DOIUrl":"10.1016/j.apsoil.2024.105765","url":null,"abstract":"<div><div>Legumes, as an important biological nitrogen (N) fixation resource, could effectively supplement N inputs to terrestrial ecosystems, especially in N-poor desert ecosystems. However, there is limited research on the N utilization strategies of different plant life forms coexisting under the “fertilizer island effect” created by leguminous shrubs. The study aims to explore the N acquisition patterns of herbaceous plants adjacent to <em>Eremosparton songoricum</em> (Litv.) Vassilcz. in the Gurbantungut Desert of northern China. In this study, the ¹⁵N isotope labeling method was used to track the N acquisition of the companion plants <em>Ceratocarpus arenarius</em> L. and <em>Centaurea pulchella</em> Ledeb. within 0–10 cm (D1), 30–40 cm (D2), 60–70 cm (D3), and 90–100 cm (D4) from a dominant legume species <em>E. songoricum</em>. The results showed that: (1) The “N acquisition” by herbaceous plants near leguminous shrubs showed distinct effects related to distance, with a preference for N-NH₄⁺ (contribution rate: 25.42 % ∼ 58.94 %) at a closer distance from the shrub, and a preference for N-NO₃⁻(contribution rate: 17.17 %–54.65 %) at a farther distance. (2) There was niche separation in the acquisition of organic N by the associated species of different life forms, in which the ¹⁵N-Glycine recovery and absorption rate of <em>Cer. arenarius</em> decreased with the increase of distance level (D1 &gt; D2 &gt; D3 &gt; D4). However, <em>Cen. pulchella</em> had the maximum ¹⁵N-Glycine recovery at D4 (90–100 cm) (D1 &lt; D2 &lt; D3 &lt; D4). (3) By analyzing the key driving factors that influence the N form preferences of non-leguminous plants associated with leguminous shrubs, it was found that the biomass of the 2 species tended to increase with increasing distance levels, and the preference for N forms changed from ammonium N to nitrate N. However, increased distance had a positive effect on the acquisition of N-Glycine and N-NO₃⁻, whereas it negatively impacted the uptake of N-NH₄⁺. These findings revealed that in N-poor desert ecosystems, herbaceous plants near leguminous shrubs adjusted their N preferences based on distance to optimize N uptake and facilitate niche coexistence. This study provides data that support a deeper understanding of the mechanisms of species coexistence and ecological stability assessments among diverse plant species in desert environments.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"205 ","pages":"Article 105765"},"PeriodicalIF":4.8,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142702179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-rate nitrogen loading accelerates organic phosphorus loss through enzymatic and non-enzymatic processes in a semi-arid grassland","authors":"Nan Jiang ,&nbsp;Qi Wang ,&nbsp;Dongqi Jiang ,&nbsp;Chenran Wu ,&nbsp;Jiahui Pu ,&nbsp;Wenjing Huang ,&nbsp;Zimeng Yao ,&nbsp;Zhenhua Chen ,&nbsp;Yulan Zhang ,&nbsp;Lijun Chen","doi":"10.1016/j.apsoil.2024.105755","DOIUrl":"10.1016/j.apsoil.2024.105755","url":null,"abstract":"<div><div>Semi-arid grasslands suffer from increased nitrogen (N) loading, which affects phosphorus (P) cycling. Phosphorus is essential but limited for all living organisms. Organic P (Po) is crucial for P supply in grasslands response to N loading, but its transformation process remains unclear. To elucidate P cycling, we evaluated P fractions, phosphatase activities, and their coding genes (<em>pho</em> genes <em>phoD</em>, <em>phoX</em>, and <em>phoC</em>) at both DNA and RNA levels in response to a nitrogen loading gradient. The results showed that the total P and Po contents significantly decreased with increasing N loading rates. In contrast, plant and litter biomass P exhibited an opposite trend but were insufficient to offset soil P loss. Unexpectedly, neither the phosphatase activities nor microbial biomass increased with increasing N loading rates, although the DNA abundance of <em>pho</em> genes responded positively to N enrichment. A partial correlation network indicated that N loading rates decreased Po levels through phosphatase activities, which were significantly associated with soil pH and the RNA abundance of <em>pho</em> genes. Moreover, non-enzymatic mineralization may be enhanced in Po cycling in semi-arid grasslands undergoing high N loading, especially following the initial stage of enzymatic mineralization. Although the activity of phosphatases did not continue to rise with increased N loading, the strategy for addressing N-induced P limitation through overconsumption of Po via non-enzymatic processes would accelerate the degradation of the ecosystem in the future.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"205 ","pages":"Article 105755"},"PeriodicalIF":4.8,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142702178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Addition of bacterial-feeding nematodes contributes to soil phosphorus availability by affecting the mineralization of moderately labile organic phosphorus","authors":"Jigao Wang ,&nbsp;Kai Wei ,&nbsp;Jialiang Tang ,&nbsp;Chaoyan Yuan ,&nbsp;Yanling Wang ,&nbsp;Xiaoming Sun ,&nbsp;Bo Zhu","doi":"10.1016/j.apsoil.2024.105764","DOIUrl":"10.1016/j.apsoil.2024.105764","url":null,"abstract":"<div><div>Improving the soil residual organic phosphorus (P) utilization rate is important for alleviating P resource shortages and reducing water pollution. Bacterial-feeding nematodes can impact soil alkaline phosphomonoesterase (AlP) activity and mediated organic P mineralization through affecting AlP-producing bacteria that harbor the <em>phoD</em> gene. However, it is unclear which organic P fraction mineralization is promoted with increasing AlP activity after bacterial-feeding nematodes are added, and whether this promotion effect varies with fertilization regimes. Microcosm experiments without (control; -Nematode) and with adding bacterial-feeding nematode <em>Eucephalobus</em> (+Nematode) were carried out in the present study to investigate impacts of bacterial-feeding nematodes on organic P mineralization by using soils collected from four fertilization regimes: non-fertilized control (CK), chemical nitrogen (N), P and potassium (K) fertilizers (NPK), crop straw plus chemical fertilizers (SNPK), pig manure plus chemical fertilizers (MNPK). After 30 days, +Nematode treatment significantly increased soil AlP activity under all the fertilization regimes, and also increased the richness index of AlP-producing bacteria as well as the relative abundances of the gram-positive (G+) bacteria Actinomycetia, <em>Streptomyces</em> and <em>Amycolatopsis</em>. Meanwhile, these three G+ bacteria showed a significant positive correlation with AlP activity, and contributed the most to the different AlP-producing bacterial community compositions under -Nematode treatment versus +Nematode treatment. Moreover, +Nematode treatment only significantly increased the labile organic P (NaHCO₃-Po) content in CK and SNPK soils, and the moderately labile organic P (NaOH I-Po) content in SNPK soil. Correlation analysis showed that the AlP activity had positive relationships with the AlP-producing bacterial richness index, community composition and NaOH I-Po, water-soluble P (NH₄Cl-Pi) contents. Partial least squares structural equation modeling (PLS-SEM) revealed that NaOH I-Po, under the direct influence of AlP, indirectly and positively influenced NH₄Cl-Pi by affecting moderately labile inorganic P (NaOH I-Pi). Overall, this study increases our understanding of the effect mechanism of bacterial-feeding nematodes on organic P mineralization, and finds that the addition of bacterial-feeding nematodes under straw returning may have a better effect in improving the soil P availability compared with their addition under other fertilization regimes.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"205 ","pages":"Article 105764"},"PeriodicalIF":4.8,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142702177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Increased diversification of crop rotation systems induces plant-beneficial rhizobiomes to facilitate crop performance","authors":"Shunran Hu ,&nbsp;Qi Shao ,&nbsp;Yongliang Chen ,&nbsp;Jan Kammenga ,&nbsp;Stefan Geisen","doi":"10.1016/j.apsoil.2024.105758","DOIUrl":"10.1016/j.apsoil.2024.105758","url":null,"abstract":"<div><div>Diversified crop rotation systems play a key role in sustainable agricultural production by increasing crop diversity and reducing management intensity. Evidence is accumulating that this diversification shapes the root-associated bacteria and fungi – the rhizobiome. However, the intimate link and feedback between crop diversification and the rhizobiome remains poorly understood. Our aim was to understand rhizobiome (bacteria and fungi) changes induced by increased crop diversification in rotation systems in a long-term field experiment, and their functional feedback on crop performance in a pot experiment. We found that increased crop diversification shapes rhizobiome composition in favour of potential plant-beneficial rhizosphere microorganisms, e.g. increases <em>Podospora</em> by up to 97 %, with a simultaneous reduction of plant pathogens, e.g. decreases <em>Fusarium</em> by up to 89 %. These diversification-induced rhizobiome shifts lead to an enhanced maize growth of up to 156 % in the subsequent pot experiment. Our findings emphasise the pivotal role of increased crop diversification in shaping plant-beneficial rhizobiomes and verify the functional importances of these rhizobiomes in enhancing subsequent crop performance. Therefore, our research highlights that crop diversification in rotation systems is needed to sustainably promote crop performance in future agroecosystems.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"205 ","pages":"Article 105758"},"PeriodicalIF":4.8,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142702193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacterial communities and tetracycline resistance genes in the soil-rice continuum upon tetracycline exposure","authors":"Qing Li ,&nbsp;Bin Zhang ,&nbsp;Yang Zhang ,&nbsp;Jian Hu ,&nbsp;Qigen Dai","doi":"10.1016/j.apsoil.2024.105762","DOIUrl":"10.1016/j.apsoil.2024.105762","url":null,"abstract":"<div><div>Edible plants are known as key vectors for transmission of antibiotic resistance genes (ARGs) to humans. However, little is known about how antibiotic exposure affects the abundance of ARGs in the soil-rice continuum. This study assessed tetracycline (TC) content, tetracycline resistance genes (TRGs), class 1 integron-integrase gene (<em>int</em>I1), and bacterial communities in the mature soil-rice continuum exposed to varying concentrations of TC (0, 50, 100, and 300 ppm). As TC exposure levels increased, the TC content in rhizosphere soils and roots increased accordingly. However, TC residue was not detected in the grains. Additionally, sample type (rhizosphere soils, roots, and grains) was the key determinant of bacterial communities and TRGs profiles in the soil-rice continuum. The bacterial communities and TRGs profiles in the rhizosphere soils and roots exposed to TC &gt;50 ppm were more dissimilar to those without TC than to those exposed to TC &lt;50 ppm. The abundance of TRGs in the soil-rice continuum increased with the TC concentrations but declined when TC &gt; 50 ppm. This decline was primarily due to changes in the abundance of <em>tet</em>X and <em>tet</em>Z. Variation partitioning analysis revealed that the TRGs profiles in rhizosphere soils is strongly affected by bacterial communities. In contrast, the variation in TRGs profiles in roots was predominantly explained by bacterial communities-TC interactions. However, the changes in TRGs profiles of grains could not be attributed to the bacterial communities, TC, or <em>int</em>I1. This study provides valuable insights into the response of TRGs profiles in the soil-rice continuum to TC exposure.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"205 ","pages":"Article 105762"},"PeriodicalIF":4.8,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142702192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Litter quality regulates cover crop litter decay alongside altered microbial facets","authors":"Guilong Li ,&nbsp;Ke Leng ,&nbsp;Wenjing Qin ,&nbsp;Xiaofen Chen ,&nbsp;Yongxin Lin ,&nbsp;Ming Liu ,&nbsp;Meng Wu ,&nbsp;Jianbo Fan ,&nbsp;Xiaoyue Wang ,&nbsp;Yuji Jiang ,&nbsp;Jia Liu","doi":"10.1016/j.apsoil.2024.105761","DOIUrl":"10.1016/j.apsoil.2024.105761","url":null,"abstract":"<div><div>Cover crops litter decay is a vital component of nutrient cycling in agroecosystems; however, how litter quality affect decay process via microbial facets at different decay stage remains unclear. Here, we performed a 112-d decay experiment using the litter of two winter cover crop species (a legume and a grass) to clarify the relationship between the temporal dynamics of litter-inhabiting microbes and litter decay in agroecosystems. Legume litter decayed faster (<em>k</em> = 0.064 ± 0.002 days⁻¹) than grass litter (<em>k</em> = 0.053 ± 0.003 days⁻¹) during the early stage (0–28 D) of decay. Wood saprotrophs and bacteria, capable of performing lignin degradation, were generally more abundant in legume litter (0.003 % and 47.77 %, respectively) than in grass litter (0.002 % and 39.24 %, respectively) during the early stage of decay. Litter quality affected the decay process through its effects on microbial functional profiles and interactions; legume litter decay increased as the abundance of specific functional guilds increased and network complexity decreased. Overall, our findings indicated that litter quality affected litter decay process and relevant litter-inhabiting microbial facets is restricted to the early stage of decay.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"204 ","pages":"Article 105761"},"PeriodicalIF":4.8,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142697232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of biochar on plant and microbial communities in landfill soil","authors":"Jiaxin Liao ,&nbsp;Guangcun Hao ,&nbsp;Haowen Guo ,&nbsp;Hongqi CHEN","doi":"10.1016/j.apsoil.2024.105749","DOIUrl":"10.1016/j.apsoil.2024.105749","url":null,"abstract":"<div><div>Ecological restoration in landfills encounters a significant challenge attributed to unfavourable soil conditions. A three-month field study was conducted to evaluate biochar effects on plant succession and bacterial community in a landfill cover. The topsoil of the cover was amended with 0, 5, and 10 % (<em>v</em>/v) peanut shell biochar. According to the measured results, adding biochar improves the soil pH, nutrition content, and organic matter up to 2 times, promoting plant growth. Bacterial richness and diversity were enhanced, and the heterogeneity of microbial communities was increased in biochar-amended cases. However, the plant diversity was reduced, and the domain plant species changed in biochar-amended soil. Biochar application strengthens the interaction between fungal communities and <em>Cyperus rotundus</em> L. by enhancing soil chemical properties. This study demonstrates biochar effects on soil-plant-microbe interactions for guiding the ecological restoration of landfill covers.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"204 ","pages":"Article 105749"},"PeriodicalIF":4.8,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142697321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Arbuscular mycorrhizal fungi regulation of soil carbon fractions in coal mining restoration: Dominance of glomalin-related soil proteins revealed by stable carbon isotopes (δ13C)","authors":"Mingchao Li ,&nbsp;Yinli Bi ,&nbsp;Kejing Yin ,&nbsp;Xinpeng Du ,&nbsp;Lexuan Tian","doi":"10.1016/j.apsoil.2024.105753","DOIUrl":"10.1016/j.apsoil.2024.105753","url":null,"abstract":"<div><div>Arbuscular mycorrhizal fungi (AMF) play a pivotal role in soil carbon cycling by orchestrating the recruitment of microbial communities and establishing hyphal networks. Understanding whether these attributes of AMF are instrumental in restoring soil carbon pools in areas disrupted by coal mining is crucial for forecasting soil organic carbon (SOC) dynamics in post-disturbance restoration sites. This study conducted a year-long, in-situ leaf decomposition experiment (comprising three shrubs: <em>Amorpha fruticose</em>, <em>Caragana</em>, and <em>Sea buckthorn</em>; two treatments: inoculated with AMF and without AMF inoculation) at the mycorrhizal restoration demonstration base in Inner Mongolia's mining area. Utilizing stable isotope technology, we investigated the mycorrhizal regulatory effects on aggregate carbon dynamics during the leaf decomposition process. Our findings showed that the presence of AMF hyphae accelerated carbon transformation within aggregates, resulting in augmented content of mineral-bound carbon (+6.1 %) and δ¹³C abundance (7.1 %) within microaggregates in <em>sea buckthorn</em> plots. Analysis employing random forest and partial least squares regression models corroborated that the regulation of mycorrhizal dynamics on aggregate carbon composition after fresh leaf input was indirectly influenced by GRSP rather than directly by AMF hyphae. In summary, our findings elucidate the evolving patterns of aggregate carbon dynamics following AMF inoculation in coal mining reclamation sites under field conditions. This underscored the efficacy of planting <em>sea buckthorn</em> and inoculating AMF during the vegetation restoration process of open-pit mine reclamation land in Inner Mongolia.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"204 ","pages":"Article 105753"},"PeriodicalIF":4.8,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142698427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustainable strategies for enhancing soil carbon sequestration and their beneficial impacts on soil fertility: A comprehensive review","authors":"Mathiyazhagan Narayanan ,&nbsp;Christo Ananth ,&nbsp;M. Ayyandurai ,&nbsp;Arivalagan Pugazhendhi ,&nbsp;Mohammed Ali Alshehri ,&nbsp;Ying Ma","doi":"10.1016/j.apsoil.2024.105752","DOIUrl":"10.1016/j.apsoil.2024.105752","url":null,"abstract":"<div><div>Soil inoculation with beneficial microbes and biochar is increasingly popular as soil structures degrade and agricultural yields must be maintained with minimal synthetic inputs. While beneficial microorganisms, including plant growth-promoting bacteria (PGPB) and arbuscular mycorrhizal fungi (AMF), and biochar are used as supplements, their application in large-scale agriculture remains limited. The current development of inoculants focuses primarily on improving soil fertility and plant growth, with insufficient attention to their potential for altering soil carbon (C) dynamics. This review explores the current understanding of microbes and biochar-based approaches and the mechanisms by which they enhance soil carbon sequestration (SCS). It addresses the roles of biochar and microbes in acting as physical barriers to C loss, promoting stable soil aggregates, and influencing soil microbial communities. The review underscores the importance of long-term field experiments, standardized methodologies, and widespread implementation of biochar and microbes for SCS, highlighting their potential to enhance soil fertility and mitigate climate change. The proposed integration of biochar and microbes is presented as a promising strategy for generating and maintaining soil C stocks. This analysis is timely, considering the challenges to global agriculture posed by declining soil fertility and the need for more effective sustainable approaches.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"204 ","pages":"Article 105752"},"PeriodicalIF":4.8,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142697320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}