Applied Biological Chemistry最新文献

{"title":"Anti-cancer effects of wistin on malignant melanoma cells","authors":"Minjoo Han,&nbsp;Seong-Ah Shin,&nbsp;Huiji Kim,&nbsp;Mi-Jeong Ahn,&nbsp;Chang Sup Lee","doi":"10.1186/s13765-025-00991-1","DOIUrl":"10.1186/s13765-025-00991-1","url":null,"abstract":"<div><p>Malignant melanoma (MM) is a severe skin cancer that arises from melanocytes, primarily caused by exposure to ultraviolet radiation. Although MM occurs less frequently than other skin cancers, its metastasis is easily activated, leading to a high mortality rate. MM incidence is gradually rising, necessitating the development of effective treatment strategies. Phytochemicals derived from plants are well recognized for their biological functions, including anticancer, anti-inflammatory, and antibacterial activities. However, the anticancer activity of wistin, an isoflavone, in MM remains unknown. This study aimed to investigate the anticancer effects of wistin on MM by elucidating its underlying molecular mechanisms. In this study, wistin significantly inhibited the cell viability and proliferation of B16 F10 melanoma cells. In addition, wistin induced apoptosis and cell cycle arrest and suppressed cell migration and invasion in B16 F10 cells. Moreover, our findings revealed that wistin downregulates phospho-ERK and p38. Overall, our results indicate that wistin exerts its anticancer effects by inhibiting the extracellular signal-regulated kinase and p38 mitogen-activated protein kinase pathways. Therefore, wistin could be a potential therapeutic candidate for the treatment of MM.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"68 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-025-00991-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143877690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From nature to nutrition: exploring the synergistic benefits of functional foods and herbal medicines for holistic health","authors":"Joyce Mudondo,&nbsp;Kenneth Happy,&nbsp;Roggers Gang,&nbsp;Yeongjun Ban,&nbsp;Youngmin Kang","doi":"10.1186/s13765-025-00985-z","DOIUrl":"10.1186/s13765-025-00985-z","url":null,"abstract":"<div><p>Functional foods and herbal medicines have gained global demand due to their health benefits, which have led to increased consumer interest. Their contribution to health is associated to the existence of bioactive compounds with several pharmacological properties such as antidiabetic, antimicrobial, anticancer, and antiinflammatory activities etc. Several phytochemical compounds have been reported to prevent lifestyle disorders and diseases such as cardiovascular diseases, diabetes, obesity, and hypertension. Functional foods include dairy products, bakery products and cereals, nutraceuticals, fermented foods, probiotics and prebiotics, vegetables, and fruits, whereas herbal medicines include several herbal plants not limited to ginseng, <i>ginkgo biloba</i>, and <i>ephedra</i> etc. which are expressed in many several forms such as decoctions, capsules, powders, teas, and oils. The transformation of herbal medicines from general consumption has led to the infusion of herbal extracts into foods, leading to the development of herbal functional foods including porridges, soups, beverages, biscuits, candies, chocolates, and dietary supplements. Thus, this review aims to explore the synergistic pharmacological activities of functional foods and herbal medicines as well as the challenges shaping the industry. Following the growing demand of functional foods and herbal medicines, we found that similar bioactive compounds in functional foods and herbal medicines contribute to their health benefits. However, critical issues regarding the regulation of functional foods and herbal medicines to establish their safety and efficacy are still present. Therefore, research on functional foods and herbal medicines is necessary to confirm their safety and efficacy and thereby attract more consumers.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"68 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-025-00985-z","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143856585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Design, synthesis, and biological evaluation of (E)-2-benzylidene-1-indanones derivatized by bioisosteric replacement of aurones","authors":"Youngshim Lee,&nbsp;Seunghyun Ahn,&nbsp;Euitaek Jung,&nbsp;Dongsoo Koh,&nbsp;Yoongho Lim,&nbsp;Young Han Lee,&nbsp;Soon Young Shin","doi":"10.1186/s13765-025-00987-x","DOIUrl":"10.1186/s13765-025-00987-x","url":null,"abstract":"","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"68 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-025-00987-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143848955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacillus megaterium GEB3 reduces accumulation of reactive oxygen species and enhances drought tolerance in peppers","authors":"Dongryeol Park,&nbsp;Jinwoo Jang,&nbsp;Geupil Jang","doi":"10.1186/s13765-025-00989-9","DOIUrl":"10.1186/s13765-025-00989-9","url":null,"abstract":"<div><p>Plant growth-promoting rhizobacteria regulate plant growth and stress tolerance by modulating endogenous developmental and physiological processes. This study examined the role of <i>Bacillus megaterium</i> GEB3 in affecting drought stress tolerance in peppers. GEB3 treatment significantly mitigated drought-induced symptoms, such as chlorosis, wilting, and leaf rolling, in both vegetative- and reproductive-stage peppers. For example, GEB3 treatment increased the number of fruits and total fruit weight by approximately 34% and 68%, respectively, compared to those in untreated control plants. We observed that GEB3 treatment reduces drought-induced reactive oxygen species (ROS) accumulation while increasing the transcriptional expression of antioxidant genes encoding <i>peroxidases</i> and <i>superoxide dismutases</i>, which are responsible for ROS removal. Furthermore, GEB3 activated the jasmonic acid (JA) response, and JA treatment alone was sufficient to reduce the accumulation of ROS and enhance pepper tolerance to drought stress. These findings suggest that <i>Bacillus megaterium</i> GEB3 increases drought tolerance in peppers through JA-mediated suppression of ROS accumulation, and may serve as a promising bioinoculant for improving crop tolerance against environmental stresses including drought.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"68 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-025-00989-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143830685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Strigolactone decreases ethylene biosynthesis in etiolated rice seedlings by reducing expression of OsACO genes","authors":"Gi Jun Mun,&nbsp;Jin Su Kim,&nbsp;Chan Hyeok Lee,&nbsp;Han Yong Lee","doi":"10.1186/s13765-025-00990-2","DOIUrl":"10.1186/s13765-025-00990-2","url":null,"abstract":"<div><p>In plants, developmental or environmental stresses activate a suite of different phytohormones that trigger biochemical and/or morphological adaptations. The gaseous phytohormone ethylene has a major effect on the plant life cycle from germination onward. Ethylene biosynthesis is tightly regulated by external and internal cues. In etiolated seedlings of Arabidopsis and rice, various phytohormones affect ethylene biosynthesis through transcriptional and/or post-transcriptional regulation of 1-aminocyclopropane-1-carboxylic acid (ACC), ACC synthases (ACS), and ACC oxidases (ACO). This study showed strigolactone also affected ethylene biosynthesis in dark-grown rice seedlings. Strigolactone treatment altered levels of <i>S-ADENOSYLMETHIONINE SYNTHASES</i> (OsSAMSs) and <i>ACC SYNTHASES</i> (OsACSs) transcripts, which encode enzymes involved in the initial steps of ethylene biosynthesis. The application of strigolactone reduced ethylene production, however, by decreasing transcription of <i>OsACO</i> genes, thus negatively affecting the final step of ethylene biosynthesis. In addition, treatment with strigolactone resulted in a phenotype in which the coleoptiles of dark-grown rice seedlings were shortened, contrary to treatment with ACC. These results reveal the tight correlation between strigolactone and ethylene biosynthesis.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"68 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-025-00990-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143809228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative efficacies of iron oxide-modified biochar and pyrite-modified biochar for simultaneous passivation of cadmium and arsenic in aqueous solutions and lettuce (Lactuca sativa. L) cultivation","authors":"Seo Yeon Kim,&nbsp;Jin Ju Lee,&nbsp;Goontaek Lee","doi":"10.1186/s13765-025-00988-w","DOIUrl":"10.1186/s13765-025-00988-w","url":null,"abstract":"<p>Effective simultaneous passivation of cationic (Cd) and anionic (As) heavy metal (metalloids) still is a critical environmental challenge. In this study, rice husk biochar was ball-milled with iron-based materials magnetite (Fe₃O₄) and pyrite (FeS₂), then re-pyrolyzed at 600^oC to produce modified biochars Fe₃O₄-BC and FeS₂-BC respectively. Short-term removal performance was evaluated after 24 h adsorption in dual-element aqueous systems where Fe₃O₄-BC displayed Cd (99.62%) and As (62.39%) removal, and FeS₂-BC displayed Cd (81.73%) and As (55.54%) removal, and BC displayed Cd (99.04%) and As (54.31%) removal. Tessier and Wenzel sequential extraction of Cd and As sorbed biochar solids revealed both modifications led to enhanced immobilization mechanisms (precipitation and complexation) absent in unmodified BC. XRD and FTIR spectra identified heavy metal precipitates and surface complexation respectively. Sorbed Cd, As was visualized with SEM-EDS. Long-term passivation effects were studied in co-contaminated soil systems with 1% (w/w) treatment with biochars, quantifying&gt; soil-to-plant heavy metal translocation by bioconcentration factors in lettuce plant, shoot, and root. Differing passivation superiority was observed for each individual metal, where Fe_,3,,O,_,4,,-BC treatment led to lowest plant Cd BCF (70.77%) while FeS,_,2,,-BC treatment resulted in lowest plant As BCF (65.72%),. Interestingly, in comparison to the control, application of unmodified BC led to,increased plant As BCF (101.03%),, suggesting biochar modification with inorganic iron materials leads to increased long-term stability by decelerating DOC release. Overall, Fe₃O₄-BC treatment appeared most effective in countering simultaneous Cd and As accumulation in edible lettuce portions,displaying shoot Cd BCF (35.33%) and shoot As BCF (9.17%).</p>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"68 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-025-00988-w","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143793174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ginsenoside Rh2 regulates cardiomyocyte autophagy-dependent apoptosis through the PI3K-Akt-mTOR signaling pathway to attenuate doxorubicin-induced cardiotoxicity","authors":"Youping Wu,&nbsp;Sheng Zhang,&nbsp;Liqiang Gu,&nbsp;Cong Xu,&nbsp;Xiaobo Lin,&nbsp;Hu Wang","doi":"10.1186/s13765-025-00986-y","DOIUrl":"10.1186/s13765-025-00986-y","url":null,"abstract":"<div><p>Doxorubicin (DOX)-induced cardiotoxicity has become a major concern and is considered a limitation for the use of DOX in oncology treatment. Ginsenoside Rh2 (Rh2) is a ginseng extract with anti-inflammatory, antioxidant and cell cycle regulating activities. The aim of this study was to investigate the mechanism of cardioprotective effects of Rh2 in DOX-induced cardiotoxicity. This study utilized network pharmacology to search for potential targets and pathways of Rh2 against doxorubicin-induced heart failure. The mechanism of Rh2 protection of myocardial tissue was further examined using a doxorubicin-formed rat model of heart failure. Network pharmacology predicted 128 potential targets for Rh2 treating to heart failure. Autophagy and apoptosis pathways play critical roles in Rh2 treatment of heart failure accessed by GO and KEGG enrichment analysis. Animal experiment results showed that Rh2 attenuated DOX-induced cardiotoxicity, normalized the morphology of cardiac tissue and reduced cardiomyocyte autophagy as well as apoptosis by up-regulation of the PI3K-AKT-mTOR signaling pathway to antagonize the effect of DOX on cardiomyocyte damage. These results suggest that Rh2 was able to inhibit DOX-activated autophagy signaling and apoptotic pathways in myocardial tissues and reduced cardiomyocyte apoptosis. It has potential effects to protect myocardial tissue as well as antagonize DOX-induced cardiotoxicity.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"68 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-025-00986-y","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143793202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of methanol, fusel alcohols, and other volatile compounds of local specialty alcoholic beverages (wine, beer, and soju) in Korea","authors":"Dayoung Jeong,&nbsp;Young-Suk Kim","doi":"10.1186/s13765-024-00975-7","DOIUrl":"10.1186/s13765-024-00975-7","url":null,"abstract":"<div><p>The most commonly consumed local specialty alcoholic beverages in South Korea are wine, beer, and <i>soju</i>. These alcoholic beverages contain a wide variety of volatile components, including methanol and fusel alcohols, due to their different raw materials, manufacturing methods, and fermentative microorganisms. GC–MS combined with solid phase micro-extraction (SPME) was utilized to establish a simultaneous analytical method for methanol, fusel alcohols, and other volatile compounds in alcoholic beverages, which included 9 wine, 10 beer, and 10 <i>soju</i> samples. A total of 221 volatile compounds were identified, consisting of 6 acetals, 14 acids, 33 alcohols, 9 aldehydes, 2 amides, 13 benzene derivatives, 5 phenols, 81 esters, 8 furans, 16 ketones, 6 sulfides, 26 terpenes, 1 pyrazine, and 1 miscellaneous. Among the three types of alcoholic beverages, wine had the highest methanol content. In case of fusel alcohols, wine, beer, and <i>soju</i> contained 25, 16, and 14 alcohol components, respectively. In general, those main volatile components of wine, beer, and <i>soju</i> were esters, alcohols, and benzene derivatives. In beer, terpenes were detected at similar levels with alcohols. Volatile compounds contributing to the distinction between <i>soju</i> and beer were some alcohols, such as propan-1-ol, 2-methylpropan-1-ol, and 3-methylbutan-1-ol, and acids, such as octanoic acid, whereas wine samples were distinguished from other types of alcoholic beverages by some esters, such as methyl acetate, ethyl (E)-but-2-enoate, ethyl 3-hydroxybutanoate, and diethyl butanedioate, and some alcohols, such as hexan-1-ol, nonan-2-ol, and nonan-1-ol.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"68 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00975-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143553818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to: Characterization and application of Biochar derived from greenhouse crop by-products for soil improvement and crop productivity in South Korea","authors":"Yu Na Lee,&nbsp;Sin Sil Kim,&nbsp;Dong Won Lee,&nbsp;Jae Hong Shim,&nbsp;Sang Ho Jeon,&nbsp;Ahn Sung Roh,&nbsp;Soon Ik Kwon,&nbsp;Dong-Cheol Seo,&nbsp;Seong Heon Kim","doi":"10.1186/s13765-025-00984-0","DOIUrl":"10.1186/s13765-025-00984-0","url":null,"abstract":"","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"68 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-025-00984-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143513402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Site-selective dimethylation of flavonoids using fusion flavonoid O-methyltransferases","authors":"Kyungha Lee,&nbsp;Seong Hee Bhoo,&nbsp;Sang-Won Lee,&nbsp;Man-Ho Cho","doi":"10.1186/s13765-025-00983-1","DOIUrl":"10.1186/s13765-025-00983-1","url":null,"abstract":"<div><p>Flavonoids are often decorated with methyl groups, which are catalyzed by flavonoid <i>O</i>-methyltransferases (FOMTs). Most FOMTs methylate flavonoids in a regiospecific manner. Because of the regiospecific nature of FOMTs, the synthesis of polymethoxyflavonoids is accomplished by multiple <i>O</i>-methylation steps. The multistep synthesis of dimethoxyflavonoids can be efficiently performed by a one-pot procedure using a multienzyme biocatalyst. For the one-pot production of dimethoxyflavonoids, fusion FOMTs were generated by the combination of two different regiospecific FOMTs. RdOMT10 (flavonoid 3-OMT), OsNOMT (flavonoid 7-OMT), and ObFOMT5 (flavonoid 4'-OMT) were used in the FOMT fusion. The fusion FOMTs (OsNOMT/ObFOMT5, OsNOMT/RdOMT10, and ObFOMT5/RdOMT10) were heterologously expressed in <i>Escherichia coli</i>. Activity assays of the recombinant fusion FOMTs demonstrated that OsNOMT/ObFOMT5, OsNOMT/RdOMT10, and ObFOMT5/RdOMT10 catalyze 7/4'-<i>O</i>-methylations, 7/3-<i>O</i>-methylations, and 4'/3-<i>O</i>-methylations of flavonoids, respectively. OsNOMT/ObFOMT5 and OsNOMT/RdOMT10 showed strong dimethylation activity towards diverse flavonoids and were therefore used in the site-selective bioconversion of flavonoids into dimethoxyflavonoids. The <i>E. coli</i> cells bearing OsNOMT/ObFOMT5 successfully converted flavonoids into 7,4'-dimethoxyflavonoids. The engineered <i>E. coli</i> expressing OsNOMT/RdOMT10 converted flavonoids into 3,7-dimethoxyflavonoids. This result indicates that the fusion FOMTs are useful multienzyme biocatalysts for the site-selective production of dimethoxyflavonoids by one-pot bioconversion.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"68 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-025-00983-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143370036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}