Applied Biological Chemistry最新文献

{"title":"Organ-specific intestinal anti-inflammatory activity in Justicia procumbens and EC-dependent lignan productivity in aerial parts","authors":"Ye Lin Kim,&nbsp;Yeong Bin Choi,&nbsp;To Quyen Truong,&nbsp;Phuong Kim Huynh,&nbsp;Mi Dam Kim,&nbsp;Won-Young Bae,&nbsp;Kyungsu Kang,&nbsp;Hee Ju Lee,&nbsp;Tae In Ahn,&nbsp;Sang Min Kim","doi":"10.1186/s13765-026-01147-5","DOIUrl":"10.1186/s13765-026-01147-5","url":null,"abstract":"<div><p>This study evaluated intestinal cell-based anti-inflammatory activity in <i>Justicia procumbens</i> and used organ-specific screening to identify plant organs relevant to functional-material production. The aerial-part extract and the representative lignans justicidin A and B reduced IL-1β-induced IL-8 production in HT-29 cells at selected non-cytotoxic concentrations. Because the aerial part comprises multiple organs that may differ in functional quality, leaves, stems, flowers, and roots were subsequently compared to determine which organs were most closely associated with the observed activity. Leaf and flower extracts showed pronounced IL-8 inhibition, whereas stem and root extracts exhibited weaker inhibitory activity. Justicidin A and B were also concentrated mainly in aerial organs, particularly flowers and leaves, indicating that the organ-specific activity pattern paralleled the distribution of the representative lignan markers. Because leaves and flowers are components of the harvestable aerial biomass, aerial parts were selected as practical target materials for cultivation-based production. To determine whether production of this functional biomass and its lignan constituents could be improved through nutrient management, plants were cultivated at electrical conductivity (EC) levels of 1.2 and 2.0 dS·m⁻¹. EC 2.0 increased aerial-part fresh weight at later harvest times and promoted root development. It also resulted in higher chlorophyll content than EC 1.2 during the later cultivation period. Electrolyte leakage did not differ significantly between EC treatments at the final harvest. Justicidin A and B yields generally increased with harvest time, and EC 2.0 significantly enhanced justicidin A yield at selected harvest times and justicidin B yield during the later cultivation period. These findings connect organ-specific functional quality with EC-controlled cultivation of <i>J. procumbens</i>.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"69 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2026-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1186/s13765-026-01147-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149338181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical composition of roman chamomile extracts and their effects on Caenorhabditis elegans","authors":"Mingqi Gao,&nbsp;Pengfei Wang,&nbsp;Huacheng Guo,&nbsp;Wenxin Liu,&nbsp;Rui Tian,&nbsp;Yihui Wang,&nbsp;Qingdan Yan,&nbsp;Lili Qu,&nbsp;Chunping Xu,&nbsp;Guangfu Huang,&nbsp;Xuewei Jia","doi":"10.1186/s13765-026-01148-4","DOIUrl":"10.1186/s13765-026-01148-4","url":null,"abstract":"<div><p>To explore the potential nematicidal activity of Roman chamomile extracts, Roman chamomile volatile oil was extracted via steam distillation (EO), ethanol extraction (EE) and petroleum ether extraction (PE). Sensory evaluation, gas chromatography-mass spectrometry (GC-MS), orthogonal partial least squares discriminant analysis (OPLS-DA), combined with Pearson correlation and partial least squares regression (PLSR) analysis were used to screen potential toxic components against nematodes in volatile oil. Meanwhile, <i>Caenorhabditis elegans</i> was used as a model organism to study the effects of Roman chamomile volatile oil on nematodes. The results showed that GC-MS detection identified abundant esters, terpenes, alcohols and other compounds in the volatile oil; among them, ester compounds contributed prominently to the intense floral and fruity notes of the oil. Roman chamomile volatile oil exerted inhibitory effects on nematodes, among which the EO showed the best efficacy. OPLS-DA screened 10 differential compounds with VIP &gt; 1 and <i>p</i> &lt; 0.05. Pearson and PLSR correlation analyses jointly indicated that 1,8-cineole, camphene, (+)-α-pinene, β-caryophyllene and pinocarvone exhibited significant correlations with the inhibitory effects on nematode physiological development and senescence. Compared with the control group, nematodes treated with the EO exhibited shortened lifespan, reduced locomotor activity, elevated intracellular ROS levels, and significantly enhanced lipofuscin autofluorescence, with more pronounced effects at higher concentrations. These findings provide a reference for the further development of botanical nematicidal products.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"69 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2026-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1186/s13765-026-01148-4.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149339703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined application of inorganic and organic fertilizers reduces global warming potential from rice paddy soil","authors":"Mu Seong Kim,&nbsp;Hyun Ho Lee,&nbsp;Geon Hyeong Lee,&nbsp;Seon Ung Chung,&nbsp;Dong Hyun Kim,&nbsp;Sang Ho Jeon,&nbsp;Seong Min Park,&nbsp;Stephanie Kulesza,&nbsp;Chang Oh Hong","doi":"10.1186/s13765-026-01143-9","DOIUrl":"10.1186/s13765-026-01143-9","url":null,"abstract":"<div><p>Soil carbon sequestration and greenhouse gas (GHG) emissions are critical factors in assessing the climate impacts of fertilization practices in rice paddy systems. This study evaluated the effects of inorganic fertilizer, composted animal manure, and their combined application on soil organic carbon dynamics, GHG emissions, net ecosystem carbon budget (NECB), global warming potential (GWP), and greenhouse gas intensity (GHGI) in a rice paddy field in South Korea. The combined application of inorganic fertilizer and compost (NPK+CM) resulted in higher cumulative CH₄ and N₂O emissions compared with single fertilization treatments. However, annual soil organic carbon change was positive only with the NPK+CM treatment, indicating enhanced soil carbon accumulation. Although greenhouse gas emissions alone suggested a higher warming impact with NPK+CM, net ecosystem carbon budget (NECB) analysis showed that this treatment achieved the greatest net carbon gain due to increased carbon inputs from fertilization and plant production. Consequently, the NPK+CM treatment exhibited the lowest net GWP among treatments. In addition, rice grain yield was highest and GHGI was lowest under the combined fertilization treatment, indicating improved production efficiency with reduced yield-scaled climate impact. These results demonstrate that evaluating fertilization practices based solely on greenhouse gas emissions may lead to misinterpretation of their overall climate impacts. An integrated assessment incorporating NECB, net GWP, and GHGI provides a more comprehensive framework for identifying climate-smart fertilization strategies that enhance crop productivity while mitigating global warming impacts in paddy soil systems.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"69 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2026-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1186/s13765-026-01143-9.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149339168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dammarane-type triterpenoids from the roots of Aglaia cucullata: mechanistic insights into the multitarget activity of shoreic acid in lung cancer","authors":"Ni Wayan Martiningsih,&nbsp;Dewa Ayu Ika Pramitha,&nbsp;Agus Budiawan Naro Putra,&nbsp;Kartika Dyah Palupi,&nbsp;Siska Elisahbet Sinaga,&nbsp;Wahyu Safriansyah,&nbsp;Endang Juliansyah,&nbsp;Rani Maharani,&nbsp; Nurlelasari,&nbsp;Sofa Fajriah,&nbsp;Unang Supratman,&nbsp;Desi Harneti","doi":"10.1186/s13765-026-01149-3","DOIUrl":"10.1186/s13765-026-01149-3","url":null,"abstract":"<div><p>The roots of <i>Aglaia cucullata</i> remain poorly explored despite the reported cytotoxic potential of dammarane-type triterpenoids obtained from the species. Therefore, this study aimed to isolate dammarane-derived triterpenoids from the roots of Indonesian <i>A. cucullata</i>, evaluate the cytotoxic activity against A549 lung cancer cells, and investigate the potential anti-non-small cell lung cancer (anti-NSCLC) mechanism of the most active compound using network pharmacology and molecular docking. The root extract was fractionated and purified by chromatography to obtain pure compounds (1–5), whose structures were determined by HR-TOF-MS, FTIR, and NMR spectroscopy and compared with previous studies. All isolated compounds were evaluated for cytotoxicity against A549 cells using the MTT assay. Network pharmacology was used to identify potential molecular targets and signaling pathways. This was followed by molecular docking to predict binding interactions of the most active compound with selected target proteins. Furthermore, five known triterpenoids derived from the dammarane framework were isolated, namely cabralealactone (1), a C27 trisnor-dammarane γ-lactone, cabraleone (2) and ocotillone (3), dammarane-type triterpenoids, and eichlerianic acid (4), as well as shoreic acid (5), seco-dammarane derivatives. Among the isolated compounds, compound 5 represented the first report from <i>A. cucullata</i> and showed weak cytotoxic activity against A549 cells (IC₅₀ = 123.05 µM) relative to compounds 1–4. Network pharmacology identified several potential targets and signaling pathways associated with NSCLC, while molecular docking predicted favorable interactions between 5 and the selected targets. The results showed that shoreic acid (5) acted as a multitarget-modulating scaffold requiring further investigation. This study reported the roots of <i>A. cucullata</i> as a source of bioactive dammarane-type triterpenoids.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"69 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2026-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1186/s13765-026-01149-3.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149339359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of silk fibroin on myoblasts differentiation in C2C12 cells","authors":"Gyeong Min Kim,&nbsp;Kyung-Wan Baek,&nbsp;Ah Young Lee,&nbsp;Tak Ko,&nbsp;Mieun Lee,&nbsp;Ji Hyun Kim,&nbsp;Hyun Young Kim","doi":"10.1186/s13765-026-01150-w","DOIUrl":"10.1186/s13765-026-01150-w","url":null,"abstract":"<div><p>Sarcopenia is characterized by the progressive loss of skeletal muscle mass and function, largely associated with impaired myogenic differentiation and protein synthesis. This study investigated the effects of silk fibroin derived from <i>Bombyx mori</i> cocoons on myogenic differentiation and myotube formation in C2C12 myoblasts and explored the underlying molecular mechanisms associated with the mTOR signaling pathway. Silk fibroin was primarily composed of glycine, alanine, and serine of free amino acids and contained branched-chain amino acids, including valine, leucine, and isoleucine. Silk fibroin treatment enhanced myotube formation and significantly increased myotube diameter, reaching 12.47 μm at 50 µg/mL compared with 9.90 μm in the control group. Furthermore, treatment with 50 µg/mL silk fibroin markedly upregulated the mRNA expression of <i>Mtor</i>,<i> Rptor</i>, and <i>Irs2</i>, whereas <i>Rictor</i>,<i> Prkaa1</i>, and<i> Prkaa2</i> expression remained unchanged. At the protein level, silk fibroin significantly increased the p-mTOR/mTOR ratio and elevated myogenin expression. Therefore, silk fibroin promoted myogenic differentiation and myotube maturation in C2C12 cells through activation of mTOR signaling, particularly the mTORC1-associated pathway. These findings suggest that silk fibroin may have potential as a functional ingredient or biomaterial for improving skeletal muscle health.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"69 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2026-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1186/s13765-026-01150-w.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149338831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Total phenolic content is associated with antioxidant activity independently of preliminary toxicity in medicinal plants from Sinaloa, Mexico: a multivariate approach","authors":"Elvia Jazmín Armenta-Gutiérrez,&nbsp;Samuel Campista-León,&nbsp;María Fernanda Aguilar-Valle,&nbsp;Irma Romelia Sánchez-Mendoza,&nbsp;Ricardo Clark-Tapia,&nbsp;Julio Montes-Ávila,&nbsp;Luz Isela Peinado-Guevara","doi":"10.1186/s13765-026-01146-6","DOIUrl":"10.1186/s13765-026-01146-6","url":null,"abstract":"<div><p>The medicinal flora of the state of Sinaloa, Mexico, is widely used by rural and highland communities; however, scientific information regarding its phytochemical profile, antioxidant capacity, and toxicological safety remains scarce and fragmentary. The present study evaluated the relationship among the qualitative phytochemical profile, total phenolic content (Folin–Ciocalteu), in vitro antioxidant capacity (DPPH, ABTS, and FRAP), and preliminary toxicity (<i>Artemia salina</i> lethality assay) of 20 ethanolic extracts obtained from 15 native medicinal species, using an integrative multivariate statistical approach (Spearman correlation, hierarchical clustering, FAMD, PLS, and PERMANOVA). A very strong positive association was found between total phenolic content and antioxidant activity (ρ = 0.72–0.92; adjusted <i>p</i> &lt; 0.005), consistently confirmed across all analytical approaches. Because the Folin–Ciocalteu assay quantifies total reducing capacity rather than phenolic compounds exclusively, this association is interpreted as a quantitative relationship between reducing power and radical-scavenging activity rather than as evidence of a specific structural class. In contrast, qualitative phytochemical screening showed no predictive capacity for antioxidant activity (η² &lt; 0.05; PERMANOVA, <i>R</i>² ≤ 0.033). The <i>Arbutus bicolor</i> and <i>Eysenhardtia polystachya</i> extracts were identified as those with the highest antioxidant potential. Preliminary toxicity and antioxidant activity exhibited functional independence (ρ ≈ 0). The data obtained for <i>A. bicolor</i> constitute the first quantitative evidence of antioxidant capacity and preliminary toxicity for this species native to the Sierra Madre Occidental. The multivariate approach employed provides a replicable analytical framework for the systematic exploration of regional medicinal flora and identifies <i>A. bicolor</i> and <i>E. polystachya</i> as priority candidates for chromatographic characterization (HPLC-DAD, LC–MS/MS).</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"69 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2026-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1186/s13765-026-01146-6.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149235137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of bamboo biochar–iron nano bio- composite for efficient removal of ceftaroline from pharmaceutical wastewater through adsorption and Fenton-like oxidation","authors":"S. Siddharth,&nbsp;Vamsikrishna kudapa,&nbsp;Vijayakumar Sivasundar,&nbsp;Itha Veeranjaneyulu,&nbsp;Kibrom Hagos Gebrehiwot,&nbsp;Movva Naga swapna sri,&nbsp;Anusha Peyyala,&nbsp;Amaleswari Rajulapati","doi":"10.1186/s13765-026-01144-8","DOIUrl":"10.1186/s13765-026-01144-8","url":null,"abstract":"<div><p>This paper aimed to design a biochar-based nanobiocomposite from bamboo to remove fifth-generation cephalosporin antibiotics, such as Ceftaroline, from pharmaceutical wastewater. Porous biochar was prepared by pyrolysis of bamboo biomass at 500 °C (BBC-A500) and 700 °C with KOH activation (BBC-Fe10), whereas iron nanoparticles were produced via a chemical reduction pathway and later linked to the biochar network. This was systematically characterized by a series of analyses, i.e., SEM, TEM, XRD, FTIR, BET, and XPS, which verified the presence of stable iron loading, a high specific surface area, and numerous surface functional groups. XPS analysis confirmed mixed Fe²⁺/Fe³⁺ surface states (Fe²⁺/Fe³⁺ ratio = 0.92), which are catalytically active for Fenton-like reactions. The degradation experiment was also conducted in batch mode to evaluate degradation performance as a function of contact time, pH, catalyst load, and oxidant concentration. This elimination was carried out in a two-step mechanism, in which preliminary adsorption concentrated Ceftaroline molecules on the biochar surface, and catalytic oxidation was conducted via Fenton-like oxidation by encapsulated iron nanoparticles. The resulting reactive species are generated to enable rapid and extensive degradation of antibiotics, and mechanistic probing indicated that hydroxyl radicals — both free and surface-bound — were the predominant oxidizing species, with minor contributions from superoxide and singlet oxygen. The composite achieved 98% removal within 60 min (k_app = 0.092 min⁻¹) and retained 82% activity after five cycles, with cumulative Fe leaching below 1.5%. Overall, the adsorption-oxidation synergy makes the bamboo biochar-iron nanocomposite a promising solution for treating pharmaceutical effluents.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"69 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1186/s13765-026-01144-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149189377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RFID-based assessment of sublethal effects of fenobucarb and carbendazim on honey bee homing flight","authors":"Bala Murali Krishna Vasamsetti,&nbsp;Kyongmi Chon,&nbsp;Juyeong Kim,&nbsp;Bo-Seon Kim,&nbsp;Chang-Young Yoon,&nbsp;Minju Choi,&nbsp;Hui-Yeon Ahn,&nbsp;Sojeong Hwang,&nbsp;Kyeong-Hun Park","doi":"10.1186/s13765-026-01145-7","DOIUrl":"10.1186/s13765-026-01145-7","url":null,"abstract":"<div><p>Assessing the sublethal behavioral effects of pesticides on honey bees (<i>Apis mellifera</i>) is a key component of honey bee risk assessment. In this study, we aimed to refine experimental conditions for RFID-based tracking and to evaluate the sublethal effects of fenobucarb (FNC) and carbendazim (CBZ) on honey bee homing performance. Individually tagged forager bees were exposed to sublethal doses of FNC (0.00025 and 0.0025 µg/bee) or CBZ (0.005 and 0.05 µg/bee) and monitored for 24 h. Behavioral endpoints, including the return rate, first-return time, and frequency of out-of-hive trips, were quantified via RFID detection at the hive entrance. Control and solvent-control groups showed high (&gt; 90%) and comparable return rates, indicating high baseline homing performance and no apparent effect of solvent exposure. FNC exposure at both doses did not significantly affect homing success, median return time, or frequency of out-of-hive trips relative to solvent exposure. At the lower dose, CBZ exposure significantly reduced the return rate; however, this effect was not observed at the higher dose and was not accompanied by changes in median return time or frequency of out-of-hive trips, suggesting the absence of a consistent or dose-dependent behavioral response. Considering the overall pattern across behavioral endpoints, no consistent adverse effects on homing-related behavior were observed for either substance under the study conditions. Overall, the refined RFID methodology provided consistent and reliable measurements of sublethal behavioral performance in honey bees.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"69 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1186/s13765-026-01145-7.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149188988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heterogeneity-guided RNA-seq meta-analysis reveals reproducible drought-responsive genes in tomato","authors":"Hongman Moon,&nbsp;Hyejin Cho,&nbsp;Chanseok Shin","doi":"10.1186/s13765-026-01137-7","DOIUrl":"10.1186/s13765-026-01137-7","url":null,"abstract":"<div><p>Drought stress severely affects tomato (<i>Solanum lycopersicum</i> L.) growth, yield, and fruit quality. Although RNA-seq has identified drought-responsive genes in tomato, differences in experimental design and stress conditions can limit the reproducibility of DEG-based results. This study performed a meta-analysis of four independent public RNA-seq datasets comprising 51 tomato leaf samples to identify drought-responsive candidate genes supported across datasets. Differential gene expression data from individual datasets were integrated with heterogeneity assessment, identifying 5,681 heterogeneity-filtered drought-responsive meta-DEGs after study-coverage and heterogeneity-based filtering. Fixed- and random-effects pooled directions agreed for 5,647 genes (99.4%), and 3,294 genes remained significant at a random-effects FDR &lt; 0.05. The prioritized candidate genes included those associated with MAPK signaling, auxin-responsive and redox regulation, phenylpropanoid, lipid, and cuticle metabolism, and proteolysis. GO enrichment of effect-size-significant meta-DEGs represented in at least three datasets identified nominally enriched terms related to protein processing, proteolysis, transport-related processes, photosynthesis, auxin response, and redox homeostasis. In ‘Micro-Tom’ plants subjected to soil-drying-induced drought stress, RT-qPCR analysis of ten representative genes revealed distinct temporal expression patterns. The drought-responsive marker genes <i>SlPP2C3</i>, <i>SlAREB1</i>, and <i>SlNCED1</i> were also induced during progressive soil drying, providing molecular evidence of a response to irrigation withdrawal. These results demonstrate that RNA-seq-based meta-analysis combined with heterogeneity assessment can prioritize drought-responsive candidates in tomato and provide a candidate set for future functional studies.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"69 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1186/s13765-026-01137-7.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149132797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"WTAP-induced upregulation of KDR ameliorates angiotensin II-induced apoptosis, oxidative stress and ferroptosis of human brain microvascular endothelial cells","authors":"Yun Shao,&nbsp;Zhengxiang Yang,&nbsp;Yi Pu,&nbsp;Xiangrong Yu,&nbsp;Weifeng Miao","doi":"10.1186/s13765-026-01140-y","DOIUrl":"10.1186/s13765-026-01140-y","url":null,"abstract":"<div><h3>Background</h3><p>Intracranial aneurysms (IAs) are a significant cause of morbidity and mortality, often leading to subarachnoid hemorrhage and subsequent neurological deficits. The kinase insert domain receptor (KDR) plays a crucial role in vascular cell function during IA. However, the mechanism responsible for KDR’s role in IA remains unclear.</p><h3>Methods</h3><p>Human brain microvascular endothelial cells (hBMECs) were treated with angiotensin II (Ang II) to simulate an in vitro IA model. Quantitative real-time PCR (qRT-PCR) was used to analyze the mRNA expression levels of KDR and Wilms’ tumor 1-associating protein (WTAP). Western blotting was employed to detect the protein expression of KDR and WTAP. Cell viability was assessed using a cell counting kit-8 assay, while cell apoptosis and mitochondrial membrane potential were measured by flow cytometry. Fluorometric assay was conducted to evaluate reactive oxygen species (ROS) levels. Colorimetric assays were used to determine the levels of reduced glutathione (GSH) and malondialdehyde (MDA), as well as Fe²⁺ levels. Methylated RNA immunoprecipitation (MeRIP) assay and dual-luciferase reporter assay were performed to validate m6A modification of KDR mRNA and the regulatory effect of WTAP on KDR.</p><h3>Results</h3><p>Treatment with Ang II induced apoptosis, oxidative stress, and ferroptosis in hBMECs. KDR and WTAP expression were significantly reduced in the arterial wall tissues of IA patients and in Ang II-treated hBMECs. Overexpression of KDR attenuated Ang II-induced apoptosis, oxidative stress, and ferroptosis in hBMECs. In addition, WTAP was found to promote KDR expression in a m6A-dependent manner. Moreover, overexpression of WTAP protected hBMECs from Ang II-induced apoptosis, oxidative stress, and ferroptosis by upregulating KDR.</p><h3>Conclusion</h3><p>WTAP-induced upregulation of KDR mitigated Ang II-induced apoptosis, oxidative stress, and ferroptosis in hBMECs. These findings suggest that targeting the WTAP-KDR axis could have significant clinical implications for the prevention and treatment of IA.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"69 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1186/s13765-026-01140-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149132628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}