Applied Biological Chemistry最新文献

{"title":"Piperine reduces hair oiliness by inhibiting adipogenesis of hair stem cells","authors":"Minyoung Im,&nbsp;Nackhyoung Kim,&nbsp;Ui-Hyun Park,&nbsp;Hyeon Ho Heo,&nbsp;Soo-Jong Um","doi":"10.1186/s13765-024-00889-4","DOIUrl":"10.1186/s13765-024-00889-4","url":null,"abstract":"<div><p>Piperine, an alkaloid compound in black pepper (<i>Piper nigrum</i>), has beneficial bioactivities. Specifically, piperine inhibits adipogenesis in 3T3-L1 cells by suppressing the transcriptional activity of PPARγ. Control of hair oiliness, which is related to adipogenic regulation, is important to prevent hair loss. Excessive sebum from the sebaceous gland (SG) can cause acne, folliculitis, or irritated skin by clogging pores. To investigate the in vivo function of piperine in SG, we used mice fed a high-fat diet (HFD). The HFD increased the size and Oil Red O (ORO) staining intensity of SG, which were significantly reduced by piperine. The HFD also upregulated the expression of sebocyte-associated genes, including PPARγ target genes, an effect reversed by piperine. In CD34/CD49f double-positive hair follicle bulge stem cells isolated from mouse vibrissae, piperine inhibited cellular adipogenesis, likely via transcriptional repression of related genes. Furthermore, piperine reduced the thickness of subcutaneous fat. In human dermal papilla cells, piperine inhibited cellular adipogenesis, as shown by the reduction in ORO staining and the downregulation of PPARγ target genes. In conclusion, piperine can be used to reduce hair greasiness by suppressing adipogenesis in hair stem cells.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00889-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140340212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of safety and efficacy of febantel and fenbendazole in fish and exposure assessment","authors":"Min Ji Kim,&nbsp;Ji Young Kim,&nbsp;Dong Woo Shin,&nbsp;Hyun-Kyung Kim","doi":"10.1186/s13765-024-00879-6","DOIUrl":"10.1186/s13765-024-00879-6","url":null,"abstract":"<div><p>Fish are susceptible to blood-sucking parasite infections, which cause severe anemia, dyspnea, and ultimately death. However, veterinary drugs available for fish to treat such infectious diseases are lacking; thus, livestock drugs have been repurposed as aquatic animal drugs. Febantel (FBT) and fenbendazole (FBZ) are representative antiparasitic agents for livestock such as cattle, swine, and poultry, and are considered suitable as aquatic animal drugs. Therefore, we investigated the safety and efficacy of FBT and FBZ in fish and performed a risk assessment to determine the maximum residue limit in fish. Most studies indicate that FBT is rapidly converted to FBZ, which is metabolized to oxfendazole and oxfendazole sulfone. FBZ was frequently detectable in the plasma and tissues (e.g., muscle, skin, and the liver) in significant quantities than other metabolites. We regarded the liver as the target organ because reversible hepatocytic changes were observed in fish after administration of 100 mg/kg FBT for 9 days. No toxicological effects, such as increased mortality or decreased appetite, were observed when the fish were administered 50 mg/kg FBT for 3 days. The efficacy of the drugs was verified in various parasites, including <i>H. heterocerca, H. okamotoi or Z. japonica</i>, and <i>M. seriolae</i>, as causative agents of beko disease through laboratory and field trials. Although toxicity studies on FBZ in fish are limited, its safety has been demonstrated from toxicity studies in a wide range of animal models. The risk from using FBT and FBZ was negligible for human health because the ratio of the estimates of dietary exposure and acceptable daily intake was 78.4%.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00879-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140328974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nutrient recycling of source-separated human faeces using biochar immobilized indigenous psychrotrophic bacteria for sustaining the agroecosystems of north-western Himalaya","authors":"Shruti Sinai Borker,&nbsp;Aman Thakur,&nbsp;Krishna Kanta Pandey,&nbsp;Pallavi Sharma,&nbsp;Vivek Manyapu,&nbsp;Abhishek Khatri,&nbsp;Rakshak Kumar","doi":"10.1186/s13765-024-00887-6","DOIUrl":"10.1186/s13765-024-00887-6","url":null,"abstract":"<div><p>The Himalayan composting toilets (CTs) offer a sustainable solution for converting human faeces (HF) into compost, supplementing the low-fertile land of the region. However, CTs face challenges such as delayed composting processes (6–8 months), increased heavy metal content, and foul odour. Therefore, the current study evaluated biochar-amended psychrotrophic bacteria for HF degradation under low-temperature conditions (10 ± 2 °C). Out of 153 psychrotrophic bacteria isolated from HF compost, 17 bacterial strains were selected based on highest and two or more hydrolytic activities. Furthermore, considering the isolation source, bacterial strains were examined for haemolytic activity, biofilm formation, cytotoxicity and seed germination assay. In total, 14 potential strains belonging to <i>Pseudomonas</i>, <i>Microbacterium</i>, <i>Arthrobacter</i>, <i>Streptomyces</i>, <i>Glutamicibacter</i>, <i>Rhodococcus</i>, <i>Serratia</i>, <i>Exiguobacterium</i>, and <i>Jeotgalicoccus</i> genera were considered safe for both human handling and plants. The composting process was conducted in modified plastic drums at 10 ± 2 °C for 90 days through two treatments: Treatment 1 (T1) involving HF, non-immobilized biochar and cocopeat, and Treatment 2 (T2) involving HF, consortium-immobilized biochar and cocopeat. The consortium-immobilized biochar (T2) degraded HF within 90 days with hemicellulose and cellulose degradation ratios of 73.9% and 62.4%, respectively (p ≤ 0.05). The compost maturation indices like C/N ratio (16.5 ± 1.85), total nitrogen (2.66 ± 0.07), total phosphate (0.4 ± 0.005), total potassium (1.8 ± 0.05) also improved in T2 treatment (p ≤ 0.05). Additionally, T2 was more effective in achieving safe levels of faecal coliforms (&lt; 1000 MPN g⁻¹) and reducing heavy metal content compared to T1. 16S rRNA amplicon-based analysis demonstrated an enhancement of bacterial community diversity in T2, with the presence of <i>Rhodococcus</i>, <i>Pseudomonas</i>, <i>Arthrobacter</i>, and <i>Streptomyces</i> at the end of the composting period promoting HF degradation. Furthermore, T2-fertilized soil showed a germination index (121 ± 0.4, p ≤ 0.05) and stimulated root, shoot and yield by 110%, 45.2%, and 288%, respectively, in pea (<i>Pisum sativum</i> var. AS-10) compared to T1 (49.6%, 19%, and 5.8%, respectively) (p ≤ 0.05). In conclusion, the developed biochar-based formulation proved effective in degrading HF at low temperatures, mitigating foul odours, reducing heavy metals, and enhancing the agronomic value of the final compost. This study presents a promising approach for the sustainable management of HF that can supplement the non-nutritive soil of high-altitude regions.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00887-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140328976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of anti-obesity effects of green, fermented, and γ-aminobutyric acid teas in a high-fat diet-induced mouse model","authors":"Seung-Jun Lee,&nbsp;Kyoung Hwan Cho,&nbsp;Jong Cheol Kim,&nbsp;Ho Jin Choo,&nbsp;Jeong-Yun Hwang,&nbsp;Hyun Chin Cho,&nbsp;Young-Sool Hah","doi":"10.1186/s13765-024-00888-5","DOIUrl":"10.1186/s13765-024-00888-5","url":null,"abstract":"<div><p>Obesity, a prevalent disease associated with numerous chronic conditions, including hyperlipidemia, hyperglycemia, diabetes, and metabolic syndrome, remains a major global health challenge. This study investigated the potential of green tea (GT), fermented tea (FT), and γ-aminobutyric acid (GABA) tea (GBT), which are rich in phytonutrients and polyphenols, for the management of obesity. Using a high-fat diet-induced obese mouse model (C57BL/6N), we explored the effect of these teas on various obesity-related parameters. The mice were categorized into five groups: normal diet with water, high-fat diet with water, and high-fat diet supplemented with GT, FT, or GBT. Over 13 weeks, we monitored body weight, perirenal and liver fat, adipocyte lipid accumulation, and key metabolic indicators, such as serum cholesterol, leptin, insulin, and fasting blood glucose. These teas contain beneficial phytochemicals such as GABA, theanine, and caffeine, and have demonstrated an enhanced antioxidant capacity, which increases the scavenging of free radicals and may reduce oxidative stress. The animal study indicated a decrease in feeding efficiency and significant reductions in body weight liver fat, epididymal fat, and perirenal fat, as well as in adipocyte lipid accumulation. Additionally, notable improvements were observed in metabolic health indicators, including reductions in serum cholesterol, leptin, insulin, and fasting blood glucose levels. Our findings revealed that GT, FT, or GBT significantly counteracted the negative effects of a high-fat diet, suggesting their potential in combating obesity and related metabolic disorders.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00888-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140297156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Murraya koenigii (L.) Sprengel seeds and pericarps in relation to their chemical profiles: new approach for multidrug resistant Acinetobacter baumannii ventilator-associated pneumonia","authors":"Riham A. El-Shiekh,&nbsp;Rana Elshimy,&nbsp;Asmaa A. Mandour,&nbsp;Hanaa A. H. Kassem,&nbsp;Amal E. Khaleel,&nbsp;Saleh Alseekh,&nbsp;Alisdair R. Fernie,&nbsp;Mohamed A. Salem","doi":"10.1186/s13765-024-00886-7","DOIUrl":"10.1186/s13765-024-00886-7","url":null,"abstract":"<div><p><i>Acinetobacter</i> <i>baumannii</i> is without a doubt one of the most problematic bacteria causing hospital-acquired nosocomial infections in today's healthcare system. To solve the high prevalence of multi-drug resistant (MDR) in <i>A.</i> <i>baumannii</i>, we investigated one of the medicinal plants traditionally used as antibacterial agent; namely <i>Murraya</i> <i>koenigii</i> (L.) Sprengel. The total methanolic extracts of seeds and pericarps were prepared and their anti-bacterial activity was assessed using the agar diffusion method and minimum inhibitory concentration (MIC) was then calculated as compared to tigecycline. Then, an in-vivo murine model was established which confirmed the promising activity of <i>M.</i> <i>koenigii</i> seeds in demonstrating anti-bacterial and anti-inflammatory actions. The histopathological study of lungs, scoring of pulmonary lesions, counting of bacterial loads after infection by multi-drug resistant <i>A.</i> <i>baumannii</i> all provided evidence to support these findings. LC–MS/MS profiling coupled to molecular networking and chemometrics detected the presence of carbazole alkaloids, and coumarins as dominate metabolites of the active seed extracts. Positively correlated metabolites to antibacterial potential were 6-(2ʹ,3ʹ-dihydroxy-3-methylbutyl)-8-prenylumbelliferone, scopoline, and 5-methoxymurrayatin. An in-silico study was also performed on the crystal structure of MurF from <i>A.</i> <i>baumannii</i> (PDB ID: 4QF5), the studied structures of the mentioned extracts revealed good docking interaction at the active site suggestive of competition with the ATP ligand. These collective findings suggest that extracts of <i>Murraya</i> <i>koenigii</i> (L.) Sprengel seed is a novel prospective for the discovery of drug candidates against infections caused by MDR <i>A.</i> <i>baumannii</i>.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00886-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140291351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and application of a high-performance liquid chromatography diode-array detection (HPLC–DAD) method for the simultaneous quantification of phenolic compounds in the aerial part of Glehnia littoralis","authors":"Yun Ji Park,&nbsp;Yeong Bin Choi,&nbsp;Sang-Bin Oh,&nbsp;Jinyoung Moon,&nbsp;To Quyen Truong,&nbsp;Phuong Kim Huynh,&nbsp;Sang Min Kim","doi":"10.1186/s13765-024-00884-9","DOIUrl":"10.1186/s13765-024-00884-9","url":null,"abstract":"<div><p><i>Glehnia littoralis</i>, a medicinal herb employed in traditional practices for alleviating fatigue, cough, and a dry throat, is recognized for its beneficial properties due to a diverse array of active compounds found in its extracts. For example, the <i>G. littoralis</i> roots (Radix Glehniae) mainly contain coumarins and phenolic acids, serving as the primary focus of this study. Despite the widespread use of the tools in various industries and the development of multiple analytical methods for their examination, the edible aerial parts have industrial potential, and there is currently no analytical method available to identify their key components. In this study, a high-performance liquid chromatography method combined with diode array detection (HPLC–DAD) was developed to simultaneously detect 16 phenolic compounds previously reported to be present in the edible aerial parts of <i>G. littoralis</i>. The proposed approach included using gradient elution to change the solvent system from water/acetonitrile to water/methanol. Furthermore, the method validation was conducted, assessing its linearity, limit of detection, limit of quantification, precision, accuracy, and recovery, all of which demonstrated satisfactory results. Subsequently, the developed method was applied to quantify the phenolic compounds in various <i>G. littoralis</i> samples obtained from different organs, solvent extraction processes, and processing methods. Moreover, the online HPLC-ABTS (2,2ʹ-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) assay was used to evaluate the antioxidant capacities of individual constituents, identifying four important antioxidants and estimate the overall antioxidant capacity of the <i>G. littoralis</i> extract.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00884-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140188472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation analysis between artemisinin and its derivative contents and trichome characteristics from different Artemisia species","authors":"Yun Ji Park,&nbsp;To Quyen Truong,&nbsp;Yeong Bin Choi,&nbsp;Phuong Kim Huynh,&nbsp;Jinyoung Moon,&nbsp;Song Yi Koo,&nbsp;Hyoung Seok Kim,&nbsp;Sang Min Kim","doi":"10.1186/s13765-024-00883-w","DOIUrl":"10.1186/s13765-024-00883-w","url":null,"abstract":"<div><p><i>Artemisia</i> species have significant commercial, medical, and economic value and are widely used in the traditional medicine and pharmaceutical industries. Artemisinin, a powerful antimalarial agent, is an important pharmaceutical metabolite that primarily accumulates within the glandular trichomes (GTs) on the leaf surface of <i>Artemisia</i> plants. Trichomes arising from the elongation of epidermal cells can be classified into GTs and non-glandular trichomes (NGTs) based on their morphology. GTs and NGTs are present in <i>Artemisia</i> species, and the relationship between GTs and artemisinin has been extensively studied; however, the correlation between NGTs and artemisinin remains relatively unexplored. In this study, we inferred artemisinin derivatives and trichome characteristics based on the type of species, developmental stage, and leaf age and conducted correlation analyses to investigate the factors influencing artemisinin content across different <i>Artemisia</i> species. Artemisinin and its derivatives exhibited variations in distribution based on species and leaf age, with a decreasing trend observed across most species as the developmental stage progressed. Noticeable differences among <i>Artemisia</i> species were observed in leaf shape, morphology, and trichome distribution. Although the observed data did not evidently differentiate between species, developmental stage, and leaf age groups, principal component analysis revealed that artemisinin was positively associated with the NGTs density, indicating a correlation coefficient of 0.56 (<i>p</i> &lt; 0.0001). Therefore, the number of NGTs may affect the artemisinin content in different <i>Artemisia</i> species.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00883-w","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140188473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beneficial effects of cannabidiol from Cannabis","authors":"Sullim Lee,&nbsp;Yunjeong Lee,&nbsp;Yunseo Kim,&nbsp;Hyunji Kim,&nbsp;Haerim Rhyu,&nbsp;Kyoungmi Yoon,&nbsp;Chang-Dae Lee,&nbsp;Sanghyun Lee","doi":"10.1186/s13765-024-00867-w","DOIUrl":"10.1186/s13765-024-00867-w","url":null,"abstract":"<div><p>Cannabis, traditionally used for recreation due to psychoactive compounds in its leaves, flowers, and seeds, has not been thoroughly explored for potential therapeutic benefits. Δ9-<i>trans</i>-Tetrahydrocannabinol, a key cannabinoid in cannabis, causes hallucinogenic effects and delirium symptoms. In contrast, cannabidiol (CBD) does not induce hallucinations and has shown effectiveness in treating symptoms of various rare, incurable diseases. Cannabis exhibits neuroprotective, anti-inflammatory, anti-thrombotic, anti-bacterial, analgesic, and antiepileptic properties, recently attracting more attention. This review aims to summarize comprehensively the impact of cannabis on human health, focusing on endocannabinoids and their receptors. It also delves into recent CBD research advancements, highlighting the compound’s potential medical applications. Overall, this paper provides valuable insights into the prospective development of medical cannabis, with a particular emphasis on CBD.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00867-w","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140164222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acetyl genistin modulates myotube differentiation and attenuates dexamethasone-induced muscle atrophy through the FoxO1/3 signaling pathway in C2C12 myotubes","authors":"Won Min Jeong,&nbsp;Seung-Jin Kwag,&nbsp;Jun Young Ha,&nbsp;Seung-Jun Lee,&nbsp;Yeong-In Choe,&nbsp;Dong Yeol Lee,&nbsp;Dong Kyu Jeong,&nbsp;Hwan Hee Bae,&nbsp;Jin-Hee Seo,&nbsp;Young-Sool Hah,&nbsp;Sang Gon Kim","doi":"10.1186/s13765-024-00885-8","DOIUrl":"10.1186/s13765-024-00885-8","url":null,"abstract":"<div><p>Muscle atrophy, a debilitating condition characterized by loss of muscle mass and strength, is a major concern in various clinical settings. Acetyl genistin (AG), a bioactive compound, was evaluated for its role in muscle cell differentiation and its potential protective effects against dexamethasone (dexa)-induced muscle atrophy. Our study demonstrated that AG significantly promoted C2C12 myotube differentiation, as evidenced by enhanced myotube width and increased fusion index. Notably, AG treatment upregulated the expression of myogenic markers, including MHC, MyoD, and MyoG. Moreover, AG displayed protective properties by attenuating dexa-induced muscle atrophy, mainly by suppressing the expression of the atrophy-related genes MAFbx and MuRF1. AG's protective effects are mechanistically attributed to its regulation of the AMPK/FoxO-dependent signaling pathway. Our results highlighted the dual benefits of AG in fostering muscle differentiation and safeguarding against muscle atrophy, positioning it as a promising agent for muscle health and therapeutic applications.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00885-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140161317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Classification of soybeans from different habitats based on metabolomic–transcriptomic integration","authors":"Jinghui Wang,&nbsp;Qiyou Zheng,&nbsp;Chenxu Wang,&nbsp;Ao Zhou","doi":"10.1186/s13765-024-00882-x","DOIUrl":"10.1186/s13765-024-00882-x","url":null,"abstract":"<div><p>Soybeans are a significant agricultural product in China, with certain geographical locations often yielding higher quality, and thus more expensive, soybean crops. In this study, metabolomics and transcriptomics analyses were conducted on soybean samples from nine regions in Heilongjiang and Liaoning Provinces using untargeted liquid chromatography–mass spectrometry (LC–MS) and Illumina sequencing technologies. The primary objective was to devise an effective and unbiased method for determining the geographical origin of each soybean variety to mitigate potential fraudulent practices. Through multidimensional and unidimensional analyses, successful identification of differentially expressed metabolites (DEMs) and differentially expressed genes (DEGs) was achieved, yielding statistically significant outcomes. Integration of the metabolomics and transcriptomics datasets facilitated the construction of a correlation network model capable of distinguishing soybeans originating from different geographical locations, leading to the identification of significant biomarkers exemplifying noteworthy distinctions. To validate the feasibility of this method in practical applications, partial least squares discriminant analysis was employed to differentiate soybean samples from the nine regions. The results convincingly showcased the applicability and reliability of this approach in accurately pinpointing the geographical origin of soybeans. Distinguishing itself from prior research in soybean traceability, this study incorporates an integrated analysis of metabolomics and transcriptomics data, thereby unveiling biomarkers that offer a more precise differentiation of soybean traits across distinct regions, thereby bridging a critical research gap within the soybean traceability domain. This innovative dual-data integration analysis methodology is poised to enhance the accuracy of soybean traceability tools and lay a new foundation for future agricultural product identification research.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00882-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140141342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}