Applied Biological Chemistry最新文献

{"title":"Anticancer activity of peptide W-0803 derived from Anoplophoa glabripennis","authors":"Jae Ho Yeom,&nbsp;Jin-Woo Lee,&nbsp;Seung Myun Hong,&nbsp;Deok Jae Lee,&nbsp;Dong Choon Park,&nbsp;Namhyun Chung","doi":"10.1186/s13765-024-00908-4","DOIUrl":"10.1186/s13765-024-00908-4","url":null,"abstract":"<div><p>Natural compounds are known as a resource of anti-cancer agents. Anti-cancer capacity toward human epithelial lung cancer cell lines (A549, H460) was examined with and without treatment of trypsin on the extract of <i>Anoplophora glabripennis</i>. IC50 values without trypsin treatment were about 21.3 and 25.0 μg/mL for H460 and A549, respectively. When the extract was treated with trypsin, the IC₅₀ values were 16.0 and 15.6 μg/mL for H460 and A549, respectively, indicating that the trypsin treatment increased the anti-cancer capacity. Because trypsin treatment increased the capacity, the extract was treated with trypsin to isolate the peptide W-0803 which has lysin (K) in C-terminal and α-helix structures. With treatment of W-0803, the cell viability decreased dose-dependently for H460 and A549 cells. Apoptosis analysis showed that the cell death with the treatment of peptide W-0803 was mainly by apoptosis. The wound-healing assay also showed that the peptide W-0803 has an inhibitory capability on cell migration of H460 and A549 cells. All these results suggest that the peptide W-0803 is an anti-cancer agent for lung cancer cell treatment.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00908-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141254715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Akkermansia muciniphila postbiotic administration mitigates choline-induced plasma Trimethylamine-N-Oxide production in mice","authors":"Hongjuan Xu,&nbsp;Xiaoyun Bian,&nbsp;Hongxing Wang,&nbsp;Lin Huang,&nbsp;Xiaoxi Chen","doi":"10.1186/s13765-024-00905-7","DOIUrl":"10.1186/s13765-024-00905-7","url":null,"abstract":"<div><h3>Background</h3><p>Trimethylamine-N-Oxide (TMAO) is believed to be linked to increased likelihood of cardiovascular disease. While probiotics have shown limited effectiveness in reducing TMAO levels, the potential of postbiotics remains underexplored. This study aimed to evaluate the impact of <i>Akkermansia muciniphila</i> (<i>A. muciniphila</i>) postbiotic administration on choline-induced TMAO production in mice by modifying the gut microbiota.</p><h3>Methods</h3><p>Female C57BL/6J mice were divided into six groups, including a control group, high-choline diet group, live <i>A. muciniphila</i> probiotic group, pasteurized <i>A. muciniphila</i> postbiotic group, sodium butyrate group, and sodium propionate group. Various measurements and analyses were conducted, including TMAO and TMA levels in serum, urine, and cecal contents, as well as the expression of FXR and FMO3 in liver tissues. Additionally, metabolic parameters, body weight, serum lipid profile, hepatic protein expression (FMO3, FXR, CutC, and CutD), and gut microbiota composition were assessed.</p><h3>Results</h3><p>Administration of <i>A. muciniphila</i> postbiotic significantly reduced choline-induced plasma TMAO levels in mice. Furthermore, improvements in serum lipid profiles and liver enzyme levels suggested potential enhancements in lipid metabolism and liver function. The study also observed modulation of specific proteins related to TMAO production and metabolism, including CutC and CutD.</p><h3>Conclusion</h3><p>The findings highlight the potential of <i>A. muciniphila</i> postbiotics as a dietary strategy for mitigating cardiovascular disease risk by modulating the gut-TMAO axis. Postbiotics, particularly <i>A. muciniphila</i>, offer advantages over probiotics and warrant further investigation for their therapeutic applications in gastrointestinal and metabolic disorders.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00905-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141182085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Natural variation in tocopherols, B vitamins, and isoflavones in seeds of 13 Korean conventional soybean varieties","authors":"Eun-Ha Kim,&nbsp;Jung-Won Jung,&nbsp;Oh Suk Yu,&nbsp;So-Young Lee,&nbsp;Myeong-Ji Kim,&nbsp;Sang-Gu Lee,&nbsp;Hyoun-Min Park,&nbsp;Yongmin Jo,&nbsp;Yongsung Joo,&nbsp;Seon-Woo Oh","doi":"10.1186/s13765-024-00896-5","DOIUrl":"10.1186/s13765-024-00896-5","url":null,"abstract":"<div><p>Soybean seeds are excellent sources of tocopherols, B vitamins, and isoflavones, which are well known for their health benefits. This study investigated the influence of environment and genotype on these constituents across 13 Korean soybean varieties cultivated in three locations during the 2017–2019 growing seasons. Statistical analyses, employing both univariate and multivariate methods, revealed significant impacts of genetic and environmental factors on the composition of tocopherols, B vitamins, and isoflavones. Through permutational univariate analysis of variance, the primary contributors to each measured component were identified. Genotype strongly influenced the levels of β- and δ-tocopherols, whereas the interaction between location and year predominantly affected α- and γ-tocopherols. Vitamin B1 content was predominantly determined by genotype, whereas B3 and B6 were influenced by annual variations. Vitamin B2 level was primarily affected by the interplay between environmental and genotypic effects. Genotype had a significant effect on isoflavone components, with the exception of daidzein. Furthermore, early maturing varieties and those with black seed coats exhibited low levels of isoflavone components and total isoflavones, suggesting a relationship between maturity group and seed coat color in isoflavone variation. These findings can be used as reference values for compositional equivalence assessment of genetically modified soybeans.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00896-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141164773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the potential of Nauclea latifolia for sustainable synthesis of ZnO nanoparticles: characterization and antibacterial assessment","authors":"Segun Michael Abegunde,&nbsp;Emmanuel Folorunso Olasehinde,&nbsp;Matthew Ayorinde Adebayo","doi":"10.1186/s13765-024-00902-w","DOIUrl":"10.1186/s13765-024-00902-w","url":null,"abstract":"<div><p>The work presents a report on Zinc oxide nanoparticles (ZnO NPs) synthesized through a green approach using <i>Nauclea latifolia</i> fruit extracts, with a view to investigating the prepared nanoparticles for their antimicrobial activities. The ZnO NPs synthesized were characterized using various analytical instruments, including X-ray Diffraction (XRD), Fourier Transform Infrared (FTIR), Ultraviolet-Visible (UV-Vis) spectroscopy, Dynamic Light Scattering (DLS), and Transmission Electron Microscopy (TEM). The instruments provided valuable information on the characteristics of the Zn ONPs. The antibacterial activities of the synthesized ZnO NPs were evaluated with <i>Staphylococcus aureus (S. aureus)</i> and <i>Escherichia coli (E</i>. <i>coli)</i>. The maximum absorption was observed at 379 nm. The average hydrodynamic size and the polydispersity index (PDI) were measured as 81.77 nm and 0.401, respectively. The nanomaterial has a hexagonal wurtzite structure, and the Zn–O bond was detected at 537 cm^–1. The nanoparticles were in the nano range with sizes ranging from 10.02 nm to 28.50 nm. The <i>N. latifolia</i> fruit extract-mediated ZnO NPs showed excellent performance against the two bacteria at all concentrations of ZnO NPs. The highest inhibition zones for <i>E. coli</i> and <i>S. aureus</i> at 8 mg/L of ZnO NPs are 21 and 16 mm, respectively. This study provides valuable insights into an efficient, simple, and environmentally friendly route for synthesizing ZnO NPs with a potential application in the biomedical field.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00902-w","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141096239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Locusta migratoria hydrolysates attenuate lipopolysaccharide (LPS)/D-Galactosamine (D-Gal)-induced cytotoxicity and inflammation in Hep G2 cells via NF-κB signaling suppression","authors":"Jiaxiang Zheng,&nbsp;Sunyoon Jung,&nbsp;Jung-Heun Ha,&nbsp;Yoonhwa Jeong","doi":"10.1186/s13765-024-00900-y","DOIUrl":"10.1186/s13765-024-00900-y","url":null,"abstract":"<div><p>The prolonged state of hepatic inflammation can lead to liver damage, a critical driving force in the progression of liver-related diseases. <i>Locusta migratoria</i> (LM), an edible insect, is recognized for its protein richness and potential to produce a range of bioactive polypeptides, presenting a novel solution for liver disease. This study investigated the hepatoprotective effects of LM hydrolysates in human hepatoma G2 (Hep G2) cells challenged with lipopolysaccharide (LPS)/D-Galactosamine (D-Gal), a model of liver injury. Remarkably, LM hydrolysates significantly ameliorated cell damage, as evidenced by the inhibition of the LPS/D-Gal-induced decrease in cell viability and reduction in lactate dehydrogenase (LDH) release. Furthermore, LM hydrolysates alleviated the release of aspartate aminotransferase (AST) from cells exposed to LPS/D-Gal and lowered the secretion of inflammatory cytokines while suppressing the activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), a key pathway in inflammation. In particular, LM-N hydrolysate mitigated hepatotoxicity by attenuation of inflammatory responses to reduce interleukin 6 (IL-6) levels, and NF-κB nuclear translocation. These findings suggest that LM hydrolysates could potentially offer hepatoprotective effects by mitigating the inflammatory responses induced by LPS/D-Gal.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00900-y","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141084952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the medicinal potential of Senna siamea roots: an integrated study of antibacterial and antioxidant activities, phytochemical analysis, ADMET profiling, and molecular docking insights","authors":"Hadush Gebrehiwot,&nbsp;Urgessa Ensermu,&nbsp;Aman Dekebo,&nbsp;Milkyas Endale,&nbsp;Mo Hunsen","doi":"10.1186/s13765-024-00899-2","DOIUrl":"10.1186/s13765-024-00899-2","url":null,"abstract":"&lt;div&gt;&lt;p&gt;Nowadays, infectious diseases pose an alarming global threat to human health. The genus &lt;i&gt;Senna&lt;/i&gt; is among the most well-known taxonomic categories commonly used in folk medicine to confront these challenges. Motivated by its traditional uses, a comprehensive study was conducted on the roots extract of &lt;i&gt;Senna siamea&lt;/i&gt;, aiming to address the in vitro antibacterial and antioxidant efficacy of phytochemicals from the dichloromethane: methanol (1:1) roots extract of the plant, along with in silico computational studies. The separation of compounds was achieved using silica gel column chromatography. Whereas, the antibacterial and antioxidant activities were examined using paper disc diffusion and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays, respectively. Silica gel column chromatography of the dichloromethane: methanol (1:1) roots extract afforded lupeol (&lt;b&gt;1&lt;/b&gt;), &lt;i&gt;β&lt;/i&gt;-sitosterol (&lt;b&gt;2a&lt;/b&gt;) and stigmasterol (&lt;b&gt;2b&lt;/b&gt;), chrysophanol (&lt;b&gt;3&lt;/b&gt;), betulinic acid (&lt;b&gt;4&lt;/b&gt;), and glyceryl-1-hexacosanoate (&lt;b&gt;5&lt;/b&gt;). Although these compounds have been previously reported from the plant, proof of their medicinal applications via in vitro and in silico studies is still lacking. Notably, our findings showed remarkable inhibition zones by the extract (18.00 ± 0.00 mm and 17.17 ± 0.24 mm) against &lt;i&gt;E. coli&lt;/i&gt; and &lt;i&gt;S. aureus&lt;/i&gt;, respectively, at 50 mg/mL compared to ciprofloxacin (23.33 ± 0.47 mm and 22.00 ± 0.00 mm, respectively), showcasing its potential antibacterial efficiency. Considerable inhibition zones were also recorded by chrysophanol (&lt;b&gt;3&lt;/b&gt;) against &lt;i&gt;E. coli&lt;/i&gt; (16.33 ± 0.24 mm) and &lt;i&gt;S. pyogenes&lt;/i&gt; (16.00 ± 0.00 mm) at 2 mg/mL, compared to ciprofloxacin which showed 23.33 ± 0.47 mm and 21.67 ± 0.47 mm, respectively, signifying its potent antibacterial activities. In addition, the crude extract and chrysophanol (&lt;b&gt;3&lt;/b&gt;) exhibited substantial IC&lt;sub&gt;50&lt;/sub&gt; values (1.24 and 1.71 µg/mL, respectively), suggesting their significant antioxidant potential compared to that of ascorbic acid (IC&lt;sub&gt;50&lt;/sub&gt;: 0.53 µg/mL). Chrysophanol (&lt;b&gt;3&lt;/b&gt;) fulfilled Lipinski’s rule with no violation and lupeol (&lt;b&gt;1&lt;/b&gt;), &lt;i&gt;β&lt;/i&gt;-sitosterol (&lt;b&gt;2a&lt;/b&gt;), stigmasterol (&lt;b&gt;2b&lt;/b&gt;), betulinic acid (&lt;b&gt;4&lt;/b&gt;), and glyceryl-1-hexacosanoate (&lt;b&gt;5&lt;/b&gt;) displayed one violation each which were in favor of the drug-likeness predictions. All the compounds exhibited no cytotoxicity and except betulinic acid (&lt;b&gt;4&lt;/b&gt;), all the compounds also showed no carcinogenicity properties which were consistent with the prediction results of ciprofloxacin. The molecular docking computations revealed that all the compound isolates displayed strong and nearly strong binding affinities against all protein targets, ranging from − 6.6 kcal/mol to -9.2 kcal/mol (lupeol (&lt;b&gt;1&lt;/b&gt;) against &lt;i&gt;E. coli&lt;/i&gt; DNA gyrase B and topoisomerase II &lt;i&gt;α&lt;/i&gt;, respectively). Thus, the present findings suggest the roots of &lt;i&gt;Senna siamea&lt;/i&gt; for potential m","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00899-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141084960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zygnema sp. as creator of copper oxide nanoparticles and their application in controlling of microbial growth and photo-catalytic degradation of dyes","authors":"Sulaiman A. Alsalamah,&nbsp;Mohammed Ibrahim Alghonaim,&nbsp;Marwah Marwah Bakri,&nbsp;Tarek M. Abdelghany","doi":"10.1186/s13765-024-00891-w","DOIUrl":"10.1186/s13765-024-00891-w","url":null,"abstract":"<div><p>Recently, focus has been placed on renewable sources, as they can be provided in large quantities at the lowest possible cost, in order to create nanoparticles. One of these sources is Zygnema moss which used in the present investigation to create Copper oxide nanoparticles (CuONPs). Several phenols and flavonoids were identified the extract of Zygnema sp. via analysis of High performance liquid chromatography. These constituents served as reducing and stabilizing agents for CuONPs. Characterization of CuONPs was performed via UV-visible spectrum that demonstrated peak at 252 nm, Transmission electron microscopy that showed spherical CuONPs with mean diameter of 30.06 nm, Fourier transform infrared spectroscopy that confirm that presence of several functional groups aided to formation of CuONPs. The crystallographic pattern of CuONPs was recorded via X-ray diffraction analysis. Antimicrobial potential of CuONPs was compared to copper acetate and antibiotic/antifungal drug. CuONPs exhibited more inhibition zones against <i>S. aureus</i> (32 ± 0.1 mm), <i>E. coli</i> (36 ± 0.1 mm), <i>S. typhi</i> (27 ± 0.2 mm), <i>E. faecalis</i> (37 ± 0.1 mm), <i>C. albicans</i> (34 ± 0.3 mm) than copper acetate and antibiotic/antifungal drug. Promising MIC values of were recorded against <i>S. aureus, E. coli</i>, and <i>S. typhi</i>. CuONPs at 200 ppm inhibited the growth of <i>C. lunata</i>, <i>F. oxysporium</i>, <i>A. flavus</i>, and <i>Mucor circinelloid</i> with inhibtion of 76.92, 73.33, 63.63, and 53.84%, respectively regarded the control 100% growth. The photocatalytic role of CuONPs was recorded for degradation of reactive red (RR195) and reactive blue (RB) dyes with maximum degradation of 84.66% and 90.82%, respectively at 75 min. Moreover, the optimal dyes degradation was 84.66 and 90.82%, respectively at 40 °C.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00891-w","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141078889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of antioxidant and anti-inflammatory activity and identification of bioactive compound from the marine diatom, Odontella aurita extract","authors":"Do Manh Cuong,&nbsp;Sun Hee Yang,&nbsp;Ji Soo Kim,&nbsp;Jeong Yong Moon,&nbsp;Jongkeun Choi,&nbsp;Gyung Min Go,&nbsp;Somi Kim Cho","doi":"10.1186/s13765-024-00898-3","DOIUrl":"10.1186/s13765-024-00898-3","url":null,"abstract":"<div><p>Increased production of reactive oxygen species (ROS) leads to oxidative stress, with its damaging effect extending to the mitochondria and plasma membrane. Further, prolonged inflammation can result in chronic disease development. The marine microdiatom <i>Odontella aurita</i> is recognized for its potential in food and pharmaceutical development. Moreover, it contains antioxidant and anti-inflammatory properties. However, studies regarding the efficacy of their varying extract forms and their underlying mechanisms remain scarce. Therefore, this study aims to explore the antioxidant and anti-inflammatory effects of <i>Odontella aurita</i> extracts obtained using various extraction methods (hot water, 70% ethanol, and chloroform:methanol (CM)). Among the three <i>Odontella aurita</i> extracts, the CM extract demonstrated superior efficacy in protecting RAW 264.7 cells from H₂O₂-induced cytotoxicity. It significantly lowered the levels of ROS and enhanced the expression of superoxide dismutase and glutathione peroxidase. Furthermore, the CM extract outperformed other extracts in inhibiting LPS-induced nitric oxide production, reducing mRNA levels in nitric oxide synthase, cyclooxygenase, and the proinflammatory cytokines interleukin IL-1β, IL-6, TNFα. Additionally, CM extract effectively suppressed the activation of NF-κB/IκBα and JAK2-STAT3 in LPS-induced RAW 264.7 cells. HPLC–UV analysis revealed a remarkable 33-fold higher fucoxanthin content in CM compared to the ethanol extract. GC–MS analysis identified elevated levels of cholest-5-en-3-ol, phytol, eicosapentaenoic acid methyl ester, methyl palmitate, palmitoleic acid methyl ester, and neophytadiene in the CM extract. These findings suggest that <i>Odontella aurita</i> CM extract is a promising antioxidant candidate for preventing or treating inflammatory diseases, consequently emphasizing its potential for further development.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00898-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140924791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The potential modulatory impact of garlic-selenium nanoparticles coated with synthetic tocopherol polyethylene glycol-succinate against lead acetate toxicity in male rabbits","authors":"Mohamed Abdel Rida Yaseen,&nbsp;Madiha Hadj Ayed,&nbsp;Jabbar A. A. Al-Saaidi","doi":"10.1186/s13765-024-00893-8","DOIUrl":"10.1186/s13765-024-00893-8","url":null,"abstract":"<div><p>Toxic heavy metal lead enters in the environment due to industrial and anthropogenic activity threatens ecosystems and public health. Natural garlic extract (GE) exhibits antioxidant properties and various applications against several ailments. Therefore, this study scrutinized the protective effects of tocopherol polyethylene glycol succinate-coated garlic selenium (TPGS-GSNP) against lead acetate (LA) toxicity in rabbits. Sixty-four mature male rabbits were involved and divided into 8 equal groups. They received distilled water (negative control; T1), 30 mg/kg bw of LA (positive control; T2), 800 mg/kg bw of GE (T3), GE + LA (T4), 1 mg/kg bw of TPGS-Selenium (T5), TPGS-S + LA (T6), 1 mg/kg bw of TPGS-GSNP (T7), and TPGS-GSNP + LA (T8). Consequently, treatments were administered three times a week for 12 weeks. Following the treatment period, serum oxidant-antioxidant, protein, and lipid profiles, liver and kidney function, histopathological findings of the adrenal, liver, and kidneys, femur bone marrow chromosomal aberrations, and mitotic activity were collected and analysed. LA exposure showed significant reductions in antioxidant levels, organ weights, and mitotic activity while increasing oxidative stress, corticosteroid levels, and chromosomal aberrations. Importantly, TPGS-GSNP administration significantly improved these markers compared to the LA group. In addition, histological analysis revealed structural improvements of the studied organs in the TPGS-GSNP group compared to the LA group, which displayed high cellular necrotic and degenerative changes. In conclusion, synthetic TPGS-GSNP demonstrated higher protective efficacy against LA-induced toxicity compared to natural GE or selenium alone. However, more future studies could be conducted to explore the potential of TPGS-GSNP as an anticancer or immunomodulatory agent.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00893-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140919208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of rice hull biochar treatment on net ecosystem carbon budget and greenhouse gas emissions in Chinese cabbage cultivation on infertile soil","authors":"Do-Gyun Park,&nbsp;Hyeon-Cheol Jeong,&nbsp;Eun-Bin Jang,&nbsp;Jong-Mun Lee,&nbsp;Hyoung-Seok Lee,&nbsp;Hye-Ran Park,&nbsp;Sun-Il Lee,&nbsp;Do-Gyun Park,&nbsp;Eun-Bin Jang,&nbsp;Taek-Keun Oh","doi":"10.1186/s13765-024-00903-9","DOIUrl":"10.1186/s13765-024-00903-9","url":null,"abstract":"<div><p>Biochar, with its potential to enhance soil fertility, sequester carbon, boost crop yields and reduce greenhouse gas emissions, offers a solution. Addressing the challenges posed by climate change is crucial for food security and agriculture. However, its widespread adoption in agriculture remains in its infancy. This study assessed the effects of rice hull biochar on cabbage production and greenhouse gas emissions, especially nitrous oxide (N₂O). A trial, employing a randomized block design in triplicate was conducted from September 13 to November 23, 2022, where \"Cheongomabi\" cabbage was cultivated with N-P₂O₅-K₂O fertilization at 32<span>(-)</span>7.8<span>(-)</span>19.8 kg 10a⁻¹. Additional fertilizer was applied twice post-sowing. The Biochar application rates were control = 0 ton ha⁻¹, B1 = 1 ton ha⁻¹, B3 = 3 ton ha⁻¹, and B5 = 5 ton ha⁻¹. The aboveground biomass of autumn cabbage harvested 82 days after sowing was 2.40–2.70 kg plant⁻¹ in the control and biochar treatments (B1, B3, and B5), with no significant differences (<i>p</i> &gt; 0.05). Cumulative CO₂ emissions during cultivation varied across treatment groups, with initial and cumulative emissions of 10.40–17.94 g m⁻² day⁻¹ and 3.63–4.43 ton ha⁻¹, respectively. N₂O emissions decreased with higher biochar application: reductions of 2.9%, 25.4%, and 41.1% in the B1, B3, and B5 treatments, respectively, compared to the control. The biochar application had no significant impact on yield but curbed soil emissions, Net ecosystem carbon balance during cabbage cultivation ranged from 0.42 to 3.41 ton ha⁻¹ for the B1, B3, and B5 treatments, respectively, compared to control. Overall, the study underscores biochar’s role in mitigating emissions and boosting soil carbon during cabbage cultivation in fall.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00903-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140914583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}