Applied Biological Chemistry最新文献

{"title":"Zygnema sp. as creator of copper oxide nanoparticles and their application in controlling of microbial growth and photo-catalytic degradation of dyes","authors":"Sulaiman A. Alsalamah,&nbsp;Mohammed Ibrahim Alghonaim,&nbsp;Marwah Marwah Bakri,&nbsp;Tarek M. Abdelghany","doi":"10.1186/s13765-024-00891-w","DOIUrl":"10.1186/s13765-024-00891-w","url":null,"abstract":"<div><p>Recently, focus has been placed on renewable sources, as they can be provided in large quantities at the lowest possible cost, in order to create nanoparticles. One of these sources is Zygnema moss which used in the present investigation to create Copper oxide nanoparticles (CuONPs). Several phenols and flavonoids were identified the extract of Zygnema sp. via analysis of High performance liquid chromatography. These constituents served as reducing and stabilizing agents for CuONPs. Characterization of CuONPs was performed via UV-visible spectrum that demonstrated peak at 252 nm, Transmission electron microscopy that showed spherical CuONPs with mean diameter of 30.06 nm, Fourier transform infrared spectroscopy that confirm that presence of several functional groups aided to formation of CuONPs. The crystallographic pattern of CuONPs was recorded via X-ray diffraction analysis. Antimicrobial potential of CuONPs was compared to copper acetate and antibiotic/antifungal drug. CuONPs exhibited more inhibition zones against <i>S. aureus</i> (32 ± 0.1 mm), <i>E. coli</i> (36 ± 0.1 mm), <i>S. typhi</i> (27 ± 0.2 mm), <i>E. faecalis</i> (37 ± 0.1 mm), <i>C. albicans</i> (34 ± 0.3 mm) than copper acetate and antibiotic/antifungal drug. Promising MIC values of were recorded against <i>S. aureus, E. coli</i>, and <i>S. typhi</i>. CuONPs at 200 ppm inhibited the growth of <i>C. lunata</i>, <i>F. oxysporium</i>, <i>A. flavus</i>, and <i>Mucor circinelloid</i> with inhibtion of 76.92, 73.33, 63.63, and 53.84%, respectively regarded the control 100% growth. The photocatalytic role of CuONPs was recorded for degradation of reactive red (RR195) and reactive blue (RB) dyes with maximum degradation of 84.66% and 90.82%, respectively at 75 min. Moreover, the optimal dyes degradation was 84.66 and 90.82%, respectively at 40 °C.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00891-w","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141078889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of antioxidant and anti-inflammatory activity and identification of bioactive compound from the marine diatom, Odontella aurita extract","authors":"Do Manh Cuong,&nbsp;Sun Hee Yang,&nbsp;Ji Soo Kim,&nbsp;Jeong Yong Moon,&nbsp;Jongkeun Choi,&nbsp;Gyung Min Go,&nbsp;Somi Kim Cho","doi":"10.1186/s13765-024-00898-3","DOIUrl":"10.1186/s13765-024-00898-3","url":null,"abstract":"<div><p>Increased production of reactive oxygen species (ROS) leads to oxidative stress, with its damaging effect extending to the mitochondria and plasma membrane. Further, prolonged inflammation can result in chronic disease development. The marine microdiatom <i>Odontella aurita</i> is recognized for its potential in food and pharmaceutical development. Moreover, it contains antioxidant and anti-inflammatory properties. However, studies regarding the efficacy of their varying extract forms and their underlying mechanisms remain scarce. Therefore, this study aims to explore the antioxidant and anti-inflammatory effects of <i>Odontella aurita</i> extracts obtained using various extraction methods (hot water, 70% ethanol, and chloroform:methanol (CM)). Among the three <i>Odontella aurita</i> extracts, the CM extract demonstrated superior efficacy in protecting RAW 264.7 cells from H₂O₂-induced cytotoxicity. It significantly lowered the levels of ROS and enhanced the expression of superoxide dismutase and glutathione peroxidase. Furthermore, the CM extract outperformed other extracts in inhibiting LPS-induced nitric oxide production, reducing mRNA levels in nitric oxide synthase, cyclooxygenase, and the proinflammatory cytokines interleukin IL-1β, IL-6, TNFα. Additionally, CM extract effectively suppressed the activation of NF-κB/IκBα and JAK2-STAT3 in LPS-induced RAW 264.7 cells. HPLC–UV analysis revealed a remarkable 33-fold higher fucoxanthin content in CM compared to the ethanol extract. GC–MS analysis identified elevated levels of cholest-5-en-3-ol, phytol, eicosapentaenoic acid methyl ester, methyl palmitate, palmitoleic acid methyl ester, and neophytadiene in the CM extract. These findings suggest that <i>Odontella aurita</i> CM extract is a promising antioxidant candidate for preventing or treating inflammatory diseases, consequently emphasizing its potential for further development.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00898-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140924791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The potential modulatory impact of garlic-selenium nanoparticles coated with synthetic tocopherol polyethylene glycol-succinate against lead acetate toxicity in male rabbits","authors":"Mohamed Abdel Rida Yaseen,&nbsp;Madiha Hadj Ayed,&nbsp;Jabbar A. A. Al-Saaidi","doi":"10.1186/s13765-024-00893-8","DOIUrl":"10.1186/s13765-024-00893-8","url":null,"abstract":"<div><p>Toxic heavy metal lead enters in the environment due to industrial and anthropogenic activity threatens ecosystems and public health. Natural garlic extract (GE) exhibits antioxidant properties and various applications against several ailments. Therefore, this study scrutinized the protective effects of tocopherol polyethylene glycol succinate-coated garlic selenium (TPGS-GSNP) against lead acetate (LA) toxicity in rabbits. Sixty-four mature male rabbits were involved and divided into 8 equal groups. They received distilled water (negative control; T1), 30 mg/kg bw of LA (positive control; T2), 800 mg/kg bw of GE (T3), GE + LA (T4), 1 mg/kg bw of TPGS-Selenium (T5), TPGS-S + LA (T6), 1 mg/kg bw of TPGS-GSNP (T7), and TPGS-GSNP + LA (T8). Consequently, treatments were administered three times a week for 12 weeks. Following the treatment period, serum oxidant-antioxidant, protein, and lipid profiles, liver and kidney function, histopathological findings of the adrenal, liver, and kidneys, femur bone marrow chromosomal aberrations, and mitotic activity were collected and analysed. LA exposure showed significant reductions in antioxidant levels, organ weights, and mitotic activity while increasing oxidative stress, corticosteroid levels, and chromosomal aberrations. Importantly, TPGS-GSNP administration significantly improved these markers compared to the LA group. In addition, histological analysis revealed structural improvements of the studied organs in the TPGS-GSNP group compared to the LA group, which displayed high cellular necrotic and degenerative changes. In conclusion, synthetic TPGS-GSNP demonstrated higher protective efficacy against LA-induced toxicity compared to natural GE or selenium alone. However, more future studies could be conducted to explore the potential of TPGS-GSNP as an anticancer or immunomodulatory agent.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00893-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140919208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of rice hull biochar treatment on net ecosystem carbon budget and greenhouse gas emissions in Chinese cabbage cultivation on infertile soil","authors":"Do-Gyun Park,&nbsp;Hyeon-Cheol Jeong,&nbsp;Eun-Bin Jang,&nbsp;Jong-Mun Lee,&nbsp;Hyoung-Seok Lee,&nbsp;Hye-Ran Park,&nbsp;Sun-Il Lee,&nbsp;Do-Gyun Park,&nbsp;Eun-Bin Jang,&nbsp;Taek-Keun Oh","doi":"10.1186/s13765-024-00903-9","DOIUrl":"10.1186/s13765-024-00903-9","url":null,"abstract":"<div><p>Biochar, with its potential to enhance soil fertility, sequester carbon, boost crop yields and reduce greenhouse gas emissions, offers a solution. Addressing the challenges posed by climate change is crucial for food security and agriculture. However, its widespread adoption in agriculture remains in its infancy. This study assessed the effects of rice hull biochar on cabbage production and greenhouse gas emissions, especially nitrous oxide (N₂O). A trial, employing a randomized block design in triplicate was conducted from September 13 to November 23, 2022, where \"Cheongomabi\" cabbage was cultivated with N-P₂O₅-K₂O fertilization at 32<span>(-)</span>7.8<span>(-)</span>19.8 kg 10a⁻¹. Additional fertilizer was applied twice post-sowing. The Biochar application rates were control = 0 ton ha⁻¹, B1 = 1 ton ha⁻¹, B3 = 3 ton ha⁻¹, and B5 = 5 ton ha⁻¹. The aboveground biomass of autumn cabbage harvested 82 days after sowing was 2.40–2.70 kg plant⁻¹ in the control and biochar treatments (B1, B3, and B5), with no significant differences (<i>p</i> &gt; 0.05). Cumulative CO₂ emissions during cultivation varied across treatment groups, with initial and cumulative emissions of 10.40–17.94 g m⁻² day⁻¹ and 3.63–4.43 ton ha⁻¹, respectively. N₂O emissions decreased with higher biochar application: reductions of 2.9%, 25.4%, and 41.1% in the B1, B3, and B5 treatments, respectively, compared to the control. The biochar application had no significant impact on yield but curbed soil emissions, Net ecosystem carbon balance during cabbage cultivation ranged from 0.42 to 3.41 ton ha⁻¹ for the B1, B3, and B5 treatments, respectively, compared to control. Overall, the study underscores biochar’s role in mitigating emissions and boosting soil carbon during cabbage cultivation in fall.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00903-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140914583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights into the abundance, expression and diversity of key denitrification genes in an ecologically managed greenhouse agricultural soil","authors":"R. Hernández Maqueda,&nbsp;I. Ballesteros,&nbsp;D. Meca,&nbsp;R. Linacero,&nbsp;F. del Moral","doi":"10.1186/s13765-024-00901-x","DOIUrl":"10.1186/s13765-024-00901-x","url":null,"abstract":"<div><p>Understanding the bacteria associated with nitrification and denitrification is crucial for comprehending the processes that lead to nitrous oxide emissions in agricultural greenhouse soils. Therefore, it is important to determine their abundance and expression to gain insight into these processes.</p><p>The aim of this study was to explore the bacterial communities associated with denitrification in a greenhouse agricultural soil amended with crop residues and manure for six years. For this purpose, we proceeded to detect and quantify the genes <i>nirK</i> and <i>nirS</i> and the gene <i>nosZ</i> through clone library construction, sequencing, phylogenetic analysis, and quantitative polymerase chain reaction (qPCR). Sequence analysis based on the clone library revealed that many of the <i>nirS</i> or <i>nirK</i> genes detected were not closely related to known denitrifier bacteria, but some of the <i>nosZ</i> sequences were related to the genera such as <i>Pseudomonas</i>, <i>Halomonas</i>, and <i>Marinobacter</i>. Furthermore, the qPCR revealed a high abundance of DNA copies in <i>nirK</i>, 6.08 × 10⁹ ± 1.16 × 10⁹, while <i>nirS</i> and <i>nosZ</i> showed lower values, 9.05 × 10⁶ ± 1.65 × 10⁶ and 8.71 × 10⁶ ± 1.44 × 10⁶, respectively. However, the highest expression rate was observed for <i>nirS</i> (mRNA/DNA ratio = 3.10 × 10^− 3), while <i>nirK</i> and <i>nosZ</i> showed 10-fold lower expression rates (4.4 × 10^− 4 and 3.5 × 10^− 4, respectively). The results of this work provide a preliminary overview of the diversity, abundance and expression of key genes associated with the denitrification process in this type of soil and are a starting point for further studies to understand how this type of soil management can influence the denitrification process.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00901-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140826082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Application of CRISPR-Based C-to-G base editing in rice protoplasts","authors":"Jimin Lee,&nbsp;Nuri Oh,&nbsp;Jae‑Young Yun,&nbsp;Hee Soon Choi,&nbsp;Jang‑Kyun Seo,&nbsp;Jin‑Ho Kang,&nbsp;Choonkyun Jung","doi":"10.1186/s13765-024-00895-6","DOIUrl":"10.1186/s13765-024-00895-6","url":null,"abstract":"","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00895-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140641693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hyperoside ameliorates depression-like behavior in ovariectomized mice","authors":"Kyung Hee Hong,&nbsp;Jonghoon Jung,&nbsp;Minji Kim,&nbsp;Min Young Um","doi":"10.1186/s13765-024-00897-4","DOIUrl":"10.1186/s13765-024-00897-4","url":null,"abstract":"<div><p>The physiological changes caused by the decline in estrogen levels due to menopause are linked to an increased risk of depression. This study investigated the antidepressant effects of hyperoside (HYP), a natural flavonol glycoside, and its associated molecular mechanisms in primary hippocampal neurons and ovariectomized (OVX) mice. HYP treatment increased nitric oxide (NO) production and neuronal nitric oxide synthase (nNOS) expression in primary hippocampal neurons; additionally, it upregulated the expression of brain-derived neurotrophic factor (BDNF) and phosphorylated tropomyosin receptor kinase B (TrkB). In OVX mice, HYP treatment significantly improved depression-like behaviors in an open field test to a level comparable to estrogen treatment. Furthermore, HYP treatment upregulated OVX-induced decreased nNOS expression and BDNF-TrkB signaling in the hippocampus. Therefore, this study suggests that HYP exhibits antidepressant potential by addressing estrogen deficiency-induced alterations, specifically by restoring nNOS expression, promoting NO production, and concurrently enhancing BDNF-TrkB signaling in OVX mice.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00897-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140621284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of metabolites and in vitro hypoglycemic activity of Taiwanofungus camphoratus cultured using various methods","authors":"YongFei Ming,&nbsp;Yin Li,&nbsp;JianZhi Chu,&nbsp;XiaoShuang Zhou,&nbsp;YuXuan Huang,&nbsp;ShuDe Yang,&nbsp;YueJun Mu,&nbsp;Lin Wang,&nbsp;Rui Zhang,&nbsp;XianHao Cheng","doi":"10.1186/s13765-024-00890-x","DOIUrl":"10.1186/s13765-024-00890-x","url":null,"abstract":"<div><p><i>Taiwanofungus camphoratus</i> has attracted much attention because it can abundantly produce various active substances that exhibit blood-sugar lowering, immunity improving, and antioxidant properties. Currently, <i>T. camphoratus</i> is cultured using four main methods: cutting wood culture, solid-state fermentation, submerged fermentation, and dish culture. <i>T. camphoratus</i> produces different metabolites under different culture methods. In this study, nontargeted metabolomics was used to compare the metabolites of <i>T. camphoratus</i> produced under these four culture methods. Principal component analysis and supervised partial least squares-discriminant analysis were used to analyze the differences in the metabolites. Moreover, in vitro hypoglycemic activity of <i>T. camphoratus</i> extracts produced under four culture methods was compared by assessing their ability to inhibit the activity of α-glucosidase, α-amylase, and sucrase. A total of 186 metabolites were identified. In total, 127 metabolites were common under the four culture methods. Under solid-state fermentation, submerged fermentation, and cutting wood culture, 12, 1, and 4 metabolites were unique, respectively. The differential metabolites produced by <i>T. camphoratus</i> under four culture methods were mainly triterpenoids, phenolic compounds, and fatty acid compounds. α-glucosidase, α-amylase, and sucrase activity inhibition was the best using <i>T. camphoratus</i> extract obtained under cutting wood culture; the inhibition rates were 55.97%, 51.96%, and 78.02%, respectively, which were comparable to those exhibited by 0.001, 3, and 12 mg/mL acarbose (positive control). The metabolites produced by <i>T. camphoratus</i> and α-glucosidase, α-amylase, and sucrase inhibitory activities were different under the four culture methods. Cutting wood culture exhibited the best enzyme inhibitory activity. This study provided a theoretical basis for further use and development of various culture methods for the rational production of active metabolites of <i>T. camphoratus</i>.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00890-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140537489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Piperine reduces hair oiliness by inhibiting adipogenesis of hair stem cells","authors":"Minyoung Im,&nbsp;Nackhyoung Kim,&nbsp;Ui-Hyun Park,&nbsp;Hyeon Ho Heo,&nbsp;Soo-Jong Um","doi":"10.1186/s13765-024-00889-4","DOIUrl":"10.1186/s13765-024-00889-4","url":null,"abstract":"<div><p>Piperine, an alkaloid compound in black pepper (<i>Piper nigrum</i>), has beneficial bioactivities. Specifically, piperine inhibits adipogenesis in 3T3-L1 cells by suppressing the transcriptional activity of PPARγ. Control of hair oiliness, which is related to adipogenic regulation, is important to prevent hair loss. Excessive sebum from the sebaceous gland (SG) can cause acne, folliculitis, or irritated skin by clogging pores. To investigate the in vivo function of piperine in SG, we used mice fed a high-fat diet (HFD). The HFD increased the size and Oil Red O (ORO) staining intensity of SG, which were significantly reduced by piperine. The HFD also upregulated the expression of sebocyte-associated genes, including PPARγ target genes, an effect reversed by piperine. In CD34/CD49f double-positive hair follicle bulge stem cells isolated from mouse vibrissae, piperine inhibited cellular adipogenesis, likely via transcriptional repression of related genes. Furthermore, piperine reduced the thickness of subcutaneous fat. In human dermal papilla cells, piperine inhibited cellular adipogenesis, as shown by the reduction in ORO staining and the downregulation of PPARγ target genes. In conclusion, piperine can be used to reduce hair greasiness by suppressing adipogenesis in hair stem cells.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00889-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140340212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of safety and efficacy of febantel and fenbendazole in fish and exposure assessment","authors":"Min Ji Kim,&nbsp;Ji Young Kim,&nbsp;Dong Woo Shin,&nbsp;Hyun-Kyung Kim","doi":"10.1186/s13765-024-00879-6","DOIUrl":"10.1186/s13765-024-00879-6","url":null,"abstract":"<div><p>Fish are susceptible to blood-sucking parasite infections, which cause severe anemia, dyspnea, and ultimately death. However, veterinary drugs available for fish to treat such infectious diseases are lacking; thus, livestock drugs have been repurposed as aquatic animal drugs. Febantel (FBT) and fenbendazole (FBZ) are representative antiparasitic agents for livestock such as cattle, swine, and poultry, and are considered suitable as aquatic animal drugs. Therefore, we investigated the safety and efficacy of FBT and FBZ in fish and performed a risk assessment to determine the maximum residue limit in fish. Most studies indicate that FBT is rapidly converted to FBZ, which is metabolized to oxfendazole and oxfendazole sulfone. FBZ was frequently detectable in the plasma and tissues (e.g., muscle, skin, and the liver) in significant quantities than other metabolites. We regarded the liver as the target organ because reversible hepatocytic changes were observed in fish after administration of 100 mg/kg FBT for 9 days. No toxicological effects, such as increased mortality or decreased appetite, were observed when the fish were administered 50 mg/kg FBT for 3 days. The efficacy of the drugs was verified in various parasites, including <i>H. heterocerca, H. okamotoi or Z. japonica</i>, and <i>M. seriolae</i>, as causative agents of beko disease through laboratory and field trials. Although toxicity studies on FBZ in fish are limited, its safety has been demonstrated from toxicity studies in a wide range of animal models. The risk from using FBT and FBZ was negligible for human health because the ratio of the estimates of dietary exposure and acceptable daily intake was 78.4%.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00879-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140328974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}