Applied Biological Chemistry最新文献

{"title":"Synthesis, biochemistry, and in silico investigations of isatin-based hydrazone derivatives as monoamine oxidase inhibitors","authors":"Naseer Maliyakkal,&nbsp;Jong Min Oh,&nbsp;Sunil Kumar,&nbsp;Prashant Gahori,&nbsp;Anandkumar Tengli,&nbsp;Asmy Appadath Beeran,&nbsp;Hoon Kim,&nbsp;Bijo Mathew","doi":"10.1186/s13765-024-00917-3","DOIUrl":"10.1186/s13765-024-00917-3","url":null,"abstract":"<div><p>Ten isatin-based hydrazone derivatives were synthesized using two subseries, <b>IA</b> (isatin + acetophenone) and <b>IB</b> (isatin + benzaldehyde), and evaluated for their monoamine oxidases (MAOs) inhibitory activity. All the compounds showed stronger MAO-A inhibition than MAO-B, and the <b>IB</b> series showed more effective MAO-A inhibitory activity than <b>IA</b> series. Compound <b>IB4</b> most potently inhibited MAO-A (half maximal inhibitory concentration IC₅₀ = 0.015 µM), followed by <b>IB3</b> (IC₅₀ = 0.019 µM). On the contrary, compound <b>IB3</b> showed the highest MAO-B inhibition (IC₅₀ = 0.068 µM), followed by <b>IB4</b> (IC₅₀ = 1.87 µM). Compound <b>IB3</b> and <b>IB4</b> had low selectivity indices of 3.68 and 8.50, respectively. Structurally, the methyl group of <b>IA</b> series decreased the inhibition of both MAO-A and MAO-B. Among them, <b>IB3</b> and <b>IB4</b> (4-Cl and 4-Br in B-ring, respectively) showed higher MAO-A and MAO-B inhibition than the other substitutions. Inhibition constant K_i values of <b>IB3</b> and <b>IB4</b> for MAO-A were 0.0088 and 0.0063 µM, respectively, and those for MAO-B were 0.048 and 0.060 µM, respectively. <b>IB3</b> and <b>IB4</b> were competitive, reversible inhibitors of MAO-A and MAO-B. Molecular docking analysis predicted that <b>IB3</b> and <b>IB4</b> formed stable hydrogen bonds between Asn181 and the NH atom of isatin in the ligand-protein complex. Dynamic analysis revealed that <b>IB3</b> and <b>IB4</b> are stable with both MAO isoforms. These observations suggest <b>IB3</b> and <b>IB4</b> are potent and reversible MAO-A and MAO-B inhibitors and both compounds can be used as therapeutic agents for neurological disorders.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00917-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141624177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antidiabetic potential of Selenium nanoparticles and plasma-rich platelets in diabetic mice","authors":"Rania A. Karas,&nbsp;Shaimaa Alexeree,&nbsp;Nora Elzohery,&nbsp;Shams H. Abdel-Hafez,&nbsp;Yasser A. Attia","doi":"10.1186/s13765-024-00907-5","DOIUrl":"10.1186/s13765-024-00907-5","url":null,"abstract":"<div><p>Diabetes mellitus is a widespread endocrine disorder, which is categorized as the fourth leading cause of global mortality. Allopathic medicine has yet to provide a satisfactory cure for this condition. Consequently, there is an urgent demand for innovative antidiabetic treatment approaches with enhanced management and minimum side effects and costs. The study investigated the synergistic antidiabetic potential of combining selenium nanoparticles (Se NPs) and plasma-rich platelets (PRP) in diabetic mice. Antidiabetic activity of the proposed combination (Se NPs and PRP) was evaluated from histopathological and biochemical perspectives. The experiment involved alloxan monohydrate induced diabetic mouse model. In the in vivo study, several biochemical parameters for assessing the antidiabetic effect of the novel combination of (Se NPs and PRP) were performed such as blood glucose levels, body weight, lipid profiles, and liver damage markers (AST and ALT). Scavenging antioxidant activity was assessed by evaluation levels of hepatic and renal GSH, MDA, SOD, and CAT activities. Complete histopathological examinations of vital internal organs were carried out. Results revealed that combining Se NPs and PRP presents a novel approach for better diabetes management and reduced complications associated with the disease. These findings have therapeutic implications for managing diabetes mellitus.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00907-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141568037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-thrombotic effects of arteanoflavone by regulating cyclic nucleotides and aggregation on human platelets","authors":"Ho Keun Choi,&nbsp;Ga Yeon Kim,&nbsp;Ga Hee Lee,&nbsp;Hee su Jang,&nbsp;Da Hyeon Kang,&nbsp;Jin Pyo Lee,&nbsp;Dong-Ha Lee","doi":"10.1186/s13765-024-00914-6","DOIUrl":"10.1186/s13765-024-00914-6","url":null,"abstract":"<div><p>Excessive clotting or abnormal platelet accumulation can lead to serious cardiovascular disorders such as atherosclerosis, stroke, and thrombosis. Therefore, it is imperative to identify compounds capable of controlling or impeding platelet aggregation to prevent the onset of cardiovascular diseases. Arteanoflavone, a compound extracted from Artemisia iwayomogi, has not garnered scientific recognition for its potential health benefits, recent studies have substantiated its anti-inflammatory, antioxidant, and anti-allergic properties. However, the precise mechanisms by which arteanoflavone influences platelet aggregation and blood clot formation have not been conclusively established. This research investigates arteanoflavone’s role in these processes, particularly in platelets induced by collagen. The study reveals a significant increase in the production of cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) correlating with the administered dosage of arteanoflavone. Concurrently, a noticeable escalation is observed in substrates of cAMP-dependent kinase and cGMP-dependent kinase, specifically VASP and inositol 1,4,5-trisphosphate receptor (IP₃R).</p><p>Arteanoflavone demonstrates its ability to limit Ca²⁺ movement in the dense tubular system through IP₃R phosphorylation. Moreover, phosphorylated VASP inhibits the binding of fibrinogen to αIIb/β₃, thus suppressing platelet activity. Arteanoflavone also stimulates the phosphorylation of PI3K/Akt, a protein linked to platelet granule release, and MAPK (ERK, JNK, and p38) protein, associated with both platelet granule release and TXA₂ production.</p><p>Lastly, arteanoflavone impedes collagen-induced platelet aggregation and blood clot formation by inhibiting fibrin production in thrombin-induced platelets. Hence, it is suggested that arteanoflavone could be valuable as an agent that effectively deters platelet inhibition and blood clot formation through antiplatelet mechanisms.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00914-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141544103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DUF3055 from Staphylococcus aureus adopts unique strategy for structural distinctiveness","authors":"Hyo Jung Kim,&nbsp;Hyoun Sook Kim","doi":"10.1186/s13765-024-00913-7","DOIUrl":"10.1186/s13765-024-00913-7","url":null,"abstract":"<div><p><i>Staphylococcus aureus</i> remains a public health threat with the WHO classifying the pathogen as a high priority in the development of new antimicrobial agents. Whole genome sequencing has revealed a number of conserved genes that may be essential for cell viability and infection. Characterising the structure and function of these proteins will inevitably aid development of new antimicrobials. Therefore, this study elucidated the structure of hypothetical protein DUF3055 from <i>S. aureus</i> stain Mu50. The protein possesses an as yet undefined function and a unique fold. The size of DUF3055 made it an ideal candidate for NMR characterisation which in conjunction with circular dichroism revealed the protein to be folded. Crystallisation and structural solution found that the overall dimer fold has a negatively charged surface formed by a β-bulge and tightly crossed α-helices, with a complementary size to a DNA single turn. Our structural observations suggest that hypothetical protein DUF3055 from <i>S. aureus</i> has a role in DNA binding and gene regulation.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00913-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141495492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical fingerprint analysis of fermented Morinda citrifolia L. (Noni) juice by UHPLC Q-TOF/MS combined with chemometric analysis","authors":"Yoonjeong Kim,&nbsp;Jiye Pyeon,&nbsp;Jae-Yeon Lee,&nbsp;Eun-Min Kim,&nbsp;Im-Joung La,&nbsp;Ok-Hwan Lee,&nbsp;Keono Kim,&nbsp;Jeehye Sung,&nbsp;Younghwa Kim","doi":"10.1186/s13765-024-00910-w","DOIUrl":"10.1186/s13765-024-00910-w","url":null,"abstract":"<div><p><i>Morinda citrifolia</i> L. (Noni) has been widely used in traditional medicine in tropical zones and has become increasingly popular globally owing to its health benefits. Most noni fruits are consumed as juice, which is traditionally produced by the natural fermentation of noni fruits. In this study, the metabolic profiles of noni fruit juice (NJ1) and fermented noni fruit juices (NJ2 and NJ3) was compared. A total of 74, 83, and 91 compounds including anthraquinones, coumarins, flavonoids, phenolic acids, phenolics, terpenoids, and miscellaneous (acids, carbohydrates, vitamins, fatty acids, etc.) were tentatively identified from NJ1, NJ2, and NJ3 in both positive and negative electrospray ionization modes. The phenolic compound composition differed significantly between noni juice and fermented noni juice. The results of the unsupervised principal component analysis and hierarchical clustering analysis showed that the non-fermented juice group clustered with the fermented juice groups. Asperulosidic acid, isoasperulosidic acid, and rutin levels were higher in the NJ1 group than those in the NJ2 group. Deacetylasperulosidic acid and monotropein contents in NJ2 were higher than those in NJ1. Similarly, NJ1 had higher asperulosidic acid and isoasperulosidic acid than those in NJ3. The findings from this study have the potential to enhance the quality of fermented noni juice.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00910-w","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141448067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comprehensive database of human and livestock fecal microbiome for community-wide microbial source tracking: a case study in South Korea","authors":"Hokyung Song,&nbsp;Tatsuya Unno","doi":"10.1186/s13765-024-00915-5","DOIUrl":"10.1186/s13765-024-00915-5","url":null,"abstract":"<div><p>Fecal waste from livestock farms contains numerous pathogens, and improperly managed waste may flow into water bodies, causing water-borne diseases. Along with the popularization of high-throughput technologies, community-wide microbial source-tracking methods have been actively developed in recent years. This study aimed to construct a comprehensive fecal microbiome database for community-wide microbial source tracking and apply the database to identify contamination sources in the Miho River, South Korea. Total DNA was extracted from the samples, and the 16 S rRNA gene was amplified to characterize the microbial communities. The fecal microbiome database was validated by developing machine-learning models that predict host species based on microbial community structure. All machine learning models developed in this study showed high performance, where the area under the receiver operating characteristic curve was approximately 1. Community-wide microbial source tracking results showed a higher contribution of fecal sources to the contamination of the main streams after heavy rain. In contrast, the contribution of fecal sources remained comparatively stable in tributaries after rainfall. Considering that farms are more concentrated upstream of tributaries compared to the main streams, this result implies that the pathway for manure contaminants to reach the main streams could be groundwater rather than surface runoff. Systematic monitoring of the water quality, which encompasses river water and groundwater, should be conducted in the future. In addition, continuous efforts to identify and plug abandoned wells are necessary to prevent further water contamination.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00915-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141444746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochar supplementation altered the expression of antioxidant proteins in rice leaf chloroplasts under high-temperature stress","authors":"Min Huang,&nbsp;Xiaohong Yin,&nbsp;Jiana Chen,&nbsp;Fangbo Cao","doi":"10.1186/s13765-024-00911-9","DOIUrl":"10.1186/s13765-024-00911-9","url":null,"abstract":"<div><p>In order to identify the key antioxidant defense systems used to cope with high-temperature stress in rice leaf chloroplasts following biochar supplementation, the present study compared the expression levels of chloroplast proteins related to antioxidant defense in high-temperature stressed rice leaves between without (C0) and with biochar supplementation (C40; 40 g biochar kg^–1 soil). A total of sixteen differentially expressed antioxidant chloroplastic proteins were identified. Among them, three antioxidant enzyme proteins and eight thioredoxin proteins were 62–123% and 37–225% higher under the C40 treatment compared to C0, respectively. These results suggest that both antioxidant enzymes and the thioredoxin system are central to the biochar-mediated protection of rice leaves exposed to high-temperature stress.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00911-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141430148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomic screening of anti-inflammatory compounds in Acanthopanax sessiliflorus fruit (Ogaza) extract","authors":"Gyoung-Deuck Kim,&nbsp;Jiho Lee,&nbsp;Joong-Hyuck Auh","doi":"10.1186/s13765-024-00912-8","DOIUrl":"10.1186/s13765-024-00912-8","url":null,"abstract":"<div><p>This study investigated the anti-inflammatory compounds in Ogaza, <i>Acanthopanax sessiliflorus</i> fruit, and their extracts using metabolomic screening. Ogaza extracts were obtained in various solvents, such as 70% ethanol, 70% methanol, and water. The anti-inflammatory activity was estimated by evaluating nitric oxide production in lipopolysaccharide (LPS)-induced RAW 264.7 cells treated with the extracts. The 70% ethanol extract (EO) showed the most effective anti-inflammatory activity, inhibiting nitric oxide production by approximately 50% and downregulating iNOS expression. The 70% ethanol extract was further fractionated into three partial subfractions by preparative LC to identify the anti-inflammatory compounds. Assessment of the anti-inflammatory activity of each subfraction revealed that the third subfraction (E-F3) showed the highest inhibitory activity against nitric oxide. E-F3 effectively suppressed iNOS expression. Subsequently, liquid chromatography-tandem mass spectrometry (LC-MS/MS) and multivariate statistical analyses were performed to identify the compounds that significantly contributed to the anti-inflammatory activity of the Ogaza extract. Fourteen and 16 compounds in the negative- and positive-ion modes, respectively, were identified as significant constituents of Ogaza. Compounds like quercetin, hyperoside, acanthoside D, oleanolic acid, and scopoletin were identified as potential anti-inflammatory components in Ogaza extract. This study characterized the functional properties of <i>Acanthopanax sessiliflorus</i> fruit and indicated the possibility that other compartments of <i>Acanthopanax sessiliflorus</i> may also serve as natural sources of nutraceuticals.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00912-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141430150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel fabrication of macromolecular multi-functional hydrogel encapsulated with HUCB-derived mesenchymal stem cells to effective regeneration of cardiac repair after acute myocardial infarction","authors":"Jun Xue,&nbsp;Yu Ping Gao","doi":"10.1186/s13765-024-00904-8","DOIUrl":"10.1186/s13765-024-00904-8","url":null,"abstract":"<div><p>Acute myocardial infarction (AMI) has been treated via injectable hydrogels and biomaterial patches invented using tissue engineering advancements over the past decade. Yet the curative potential of injectable hydrogels and stem cells is limited. Here, we propose the development of an injectable and conductive hydrogel composed of oxidised macromolecular hyaluronic acid and chitosan-grafted aniline tetramer polymeric components. In an attempt to enhance the therapeutic potential of AMI therapy, mesenchymal stem cells derived from human umbilical cord blood (HUCB-MSC) have been integrated into the formulation of a conductive hydrogel. For reliable connection to the beating hearts, the hydrogel exhibited suitable adhesive properties. Hydrogel’s potent biocompatibility was determined by in vitro investigations of cell viability and proliferation of NRCMs and H9C2 cardiomyocytes. After myocardial injection, longer HUCB-MSCs survival length, cardiac functioning, and histology in SD rat myocardium were demonstrated, greatly associated by up-regulation and downregulation of cardiac-related relative gene expressions of angiogenic factors and inflammatory factors, respectively. The injectable hydrogel that contained HUCB-MSCs substantially enhanced the therapeutic benefits, indicating a potentially beneficial therapeutic approach to AMI therapy.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00904-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141424776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regional comparison study of Epimedium koreanum using UHPLC-QTOF/MS-based metabolomics approach","authors":"Alfan Danny Arbianto,&nbsp;Min Kim,&nbsp;Seon Min Oh,&nbsp;Hyun-Jae Jang,&nbsp;Hyung Won Ryu,&nbsp;Jin-Hyub Paik,&nbsp;Sei-Ryang Oh,&nbsp;Jongmin Ahn","doi":"10.1186/s13765-024-00906-6","DOIUrl":"10.1186/s13765-024-00906-6","url":null,"abstract":"<div><p>The untargeted metabolomics-based molecular networking approach combined with multivariate analysis, proves to be an effective strategy for distinguishing raw materials in herbal medicine according to specific criteria. It exhibits the correlations between chemical constituents and the geographical habitats of plants, providing a valuable tool for ensuring quality control in mass production within the industry. In this study, we conducted a comprehensive investigation of the chemical compositions of <i>Epimedium koreanum</i> Nakai and performed comparative analyses on four extracts collected from distinct regions in South Korea using untargeted metabolomics tools. Through the comprehensive use of UPLC-QTOF/MS analysis and advanced statistical techniques, we elucidated the chemical composition, leading to the identification of key chemical markers. Additionally, the molecular networking analysis revealed distinct clusters of flavonoids and phenolic acids, highlighting the influence of regional factors on the metabolite profiles. These findings offer a promising avenue for enhancing quality control and traceability in the herbal medicine industry, underscoring the important role of geographical variation in the chemical profiles of herbal products.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00906-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141326432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}