Applied Biological Chemistry最新文献

{"title":"Locusta migratoria hydrolysates attenuate lipopolysaccharide (LPS)/D-Galactosamine (D-Gal)-induced cytotoxicity and inflammation in Hep G2 cells via NF-κB signaling suppression","authors":"Jiaxiang Zheng,&nbsp;Sunyoon Jung,&nbsp;Jung-Heun Ha,&nbsp;Yoonhwa Jeong","doi":"10.1186/s13765-024-00900-y","DOIUrl":"10.1186/s13765-024-00900-y","url":null,"abstract":"<div><p>The prolonged state of hepatic inflammation can lead to liver damage, a critical driving force in the progression of liver-related diseases. <i>Locusta migratoria</i> (LM), an edible insect, is recognized for its protein richness and potential to produce a range of bioactive polypeptides, presenting a novel solution for liver disease. This study investigated the hepatoprotective effects of LM hydrolysates in human hepatoma G2 (Hep G2) cells challenged with lipopolysaccharide (LPS)/D-Galactosamine (D-Gal), a model of liver injury. Remarkably, LM hydrolysates significantly ameliorated cell damage, as evidenced by the inhibition of the LPS/D-Gal-induced decrease in cell viability and reduction in lactate dehydrogenase (LDH) release. Furthermore, LM hydrolysates alleviated the release of aspartate aminotransferase (AST) from cells exposed to LPS/D-Gal and lowered the secretion of inflammatory cytokines while suppressing the activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), a key pathway in inflammation. In particular, LM-N hydrolysate mitigated hepatotoxicity by attenuation of inflammatory responses to reduce interleukin 6 (IL-6) levels, and NF-κB nuclear translocation. These findings suggest that LM hydrolysates could potentially offer hepatoprotective effects by mitigating the inflammatory responses induced by LPS/D-Gal.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00900-y","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141084952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the medicinal potential of Senna siamea roots: an integrated study of antibacterial and antioxidant activities, phytochemical analysis, ADMET profiling, and molecular docking insights","authors":"Hadush Gebrehiwot,&nbsp;Urgessa Ensermu,&nbsp;Aman Dekebo,&nbsp;Milkyas Endale,&nbsp;Mo Hunsen","doi":"10.1186/s13765-024-00899-2","DOIUrl":"10.1186/s13765-024-00899-2","url":null,"abstract":"&lt;div&gt;&lt;p&gt;Nowadays, infectious diseases pose an alarming global threat to human health. The genus &lt;i&gt;Senna&lt;/i&gt; is among the most well-known taxonomic categories commonly used in folk medicine to confront these challenges. Motivated by its traditional uses, a comprehensive study was conducted on the roots extract of &lt;i&gt;Senna siamea&lt;/i&gt;, aiming to address the in vitro antibacterial and antioxidant efficacy of phytochemicals from the dichloromethane: methanol (1:1) roots extract of the plant, along with in silico computational studies. The separation of compounds was achieved using silica gel column chromatography. Whereas, the antibacterial and antioxidant activities were examined using paper disc diffusion and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays, respectively. Silica gel column chromatography of the dichloromethane: methanol (1:1) roots extract afforded lupeol (&lt;b&gt;1&lt;/b&gt;), &lt;i&gt;β&lt;/i&gt;-sitosterol (&lt;b&gt;2a&lt;/b&gt;) and stigmasterol (&lt;b&gt;2b&lt;/b&gt;), chrysophanol (&lt;b&gt;3&lt;/b&gt;), betulinic acid (&lt;b&gt;4&lt;/b&gt;), and glyceryl-1-hexacosanoate (&lt;b&gt;5&lt;/b&gt;). Although these compounds have been previously reported from the plant, proof of their medicinal applications via in vitro and in silico studies is still lacking. Notably, our findings showed remarkable inhibition zones by the extract (18.00 ± 0.00 mm and 17.17 ± 0.24 mm) against &lt;i&gt;E. coli&lt;/i&gt; and &lt;i&gt;S. aureus&lt;/i&gt;, respectively, at 50 mg/mL compared to ciprofloxacin (23.33 ± 0.47 mm and 22.00 ± 0.00 mm, respectively), showcasing its potential antibacterial efficiency. Considerable inhibition zones were also recorded by chrysophanol (&lt;b&gt;3&lt;/b&gt;) against &lt;i&gt;E. coli&lt;/i&gt; (16.33 ± 0.24 mm) and &lt;i&gt;S. pyogenes&lt;/i&gt; (16.00 ± 0.00 mm) at 2 mg/mL, compared to ciprofloxacin which showed 23.33 ± 0.47 mm and 21.67 ± 0.47 mm, respectively, signifying its potent antibacterial activities. In addition, the crude extract and chrysophanol (&lt;b&gt;3&lt;/b&gt;) exhibited substantial IC&lt;sub&gt;50&lt;/sub&gt; values (1.24 and 1.71 µg/mL, respectively), suggesting their significant antioxidant potential compared to that of ascorbic acid (IC&lt;sub&gt;50&lt;/sub&gt;: 0.53 µg/mL). Chrysophanol (&lt;b&gt;3&lt;/b&gt;) fulfilled Lipinski’s rule with no violation and lupeol (&lt;b&gt;1&lt;/b&gt;), &lt;i&gt;β&lt;/i&gt;-sitosterol (&lt;b&gt;2a&lt;/b&gt;), stigmasterol (&lt;b&gt;2b&lt;/b&gt;), betulinic acid (&lt;b&gt;4&lt;/b&gt;), and glyceryl-1-hexacosanoate (&lt;b&gt;5&lt;/b&gt;) displayed one violation each which were in favor of the drug-likeness predictions. All the compounds exhibited no cytotoxicity and except betulinic acid (&lt;b&gt;4&lt;/b&gt;), all the compounds also showed no carcinogenicity properties which were consistent with the prediction results of ciprofloxacin. The molecular docking computations revealed that all the compound isolates displayed strong and nearly strong binding affinities against all protein targets, ranging from − 6.6 kcal/mol to -9.2 kcal/mol (lupeol (&lt;b&gt;1&lt;/b&gt;) against &lt;i&gt;E. coli&lt;/i&gt; DNA gyrase B and topoisomerase II &lt;i&gt;α&lt;/i&gt;, respectively). Thus, the present findings suggest the roots of &lt;i&gt;Senna siamea&lt;/i&gt; for potential m","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00899-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141084960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zygnema sp. as creator of copper oxide nanoparticles and their application in controlling of microbial growth and photo-catalytic degradation of dyes","authors":"Sulaiman A. Alsalamah,&nbsp;Mohammed Ibrahim Alghonaim,&nbsp;Marwah Marwah Bakri,&nbsp;Tarek M. Abdelghany","doi":"10.1186/s13765-024-00891-w","DOIUrl":"10.1186/s13765-024-00891-w","url":null,"abstract":"<div><p>Recently, focus has been placed on renewable sources, as they can be provided in large quantities at the lowest possible cost, in order to create nanoparticles. One of these sources is Zygnema moss which used in the present investigation to create Copper oxide nanoparticles (CuONPs). Several phenols and flavonoids were identified the extract of Zygnema sp. via analysis of High performance liquid chromatography. These constituents served as reducing and stabilizing agents for CuONPs. Characterization of CuONPs was performed via UV-visible spectrum that demonstrated peak at 252 nm, Transmission electron microscopy that showed spherical CuONPs with mean diameter of 30.06 nm, Fourier transform infrared spectroscopy that confirm that presence of several functional groups aided to formation of CuONPs. The crystallographic pattern of CuONPs was recorded via X-ray diffraction analysis. Antimicrobial potential of CuONPs was compared to copper acetate and antibiotic/antifungal drug. CuONPs exhibited more inhibition zones against <i>S. aureus</i> (32 ± 0.1 mm), <i>E. coli</i> (36 ± 0.1 mm), <i>S. typhi</i> (27 ± 0.2 mm), <i>E. faecalis</i> (37 ± 0.1 mm), <i>C. albicans</i> (34 ± 0.3 mm) than copper acetate and antibiotic/antifungal drug. Promising MIC values of were recorded against <i>S. aureus, E. coli</i>, and <i>S. typhi</i>. CuONPs at 200 ppm inhibited the growth of <i>C. lunata</i>, <i>F. oxysporium</i>, <i>A. flavus</i>, and <i>Mucor circinelloid</i> with inhibtion of 76.92, 73.33, 63.63, and 53.84%, respectively regarded the control 100% growth. The photocatalytic role of CuONPs was recorded for degradation of reactive red (RR195) and reactive blue (RB) dyes with maximum degradation of 84.66% and 90.82%, respectively at 75 min. Moreover, the optimal dyes degradation was 84.66 and 90.82%, respectively at 40 °C.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00891-w","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141078889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of antioxidant and anti-inflammatory activity and identification of bioactive compound from the marine diatom, Odontella aurita extract","authors":"Do Manh Cuong,&nbsp;Sun Hee Yang,&nbsp;Ji Soo Kim,&nbsp;Jeong Yong Moon,&nbsp;Jongkeun Choi,&nbsp;Gyung Min Go,&nbsp;Somi Kim Cho","doi":"10.1186/s13765-024-00898-3","DOIUrl":"10.1186/s13765-024-00898-3","url":null,"abstract":"<div><p>Increased production of reactive oxygen species (ROS) leads to oxidative stress, with its damaging effect extending to the mitochondria and plasma membrane. Further, prolonged inflammation can result in chronic disease development. The marine microdiatom <i>Odontella aurita</i> is recognized for its potential in food and pharmaceutical development. Moreover, it contains antioxidant and anti-inflammatory properties. However, studies regarding the efficacy of their varying extract forms and their underlying mechanisms remain scarce. Therefore, this study aims to explore the antioxidant and anti-inflammatory effects of <i>Odontella aurita</i> extracts obtained using various extraction methods (hot water, 70% ethanol, and chloroform:methanol (CM)). Among the three <i>Odontella aurita</i> extracts, the CM extract demonstrated superior efficacy in protecting RAW 264.7 cells from H₂O₂-induced cytotoxicity. It significantly lowered the levels of ROS and enhanced the expression of superoxide dismutase and glutathione peroxidase. Furthermore, the CM extract outperformed other extracts in inhibiting LPS-induced nitric oxide production, reducing mRNA levels in nitric oxide synthase, cyclooxygenase, and the proinflammatory cytokines interleukin IL-1β, IL-6, TNFα. Additionally, CM extract effectively suppressed the activation of NF-κB/IκBα and JAK2-STAT3 in LPS-induced RAW 264.7 cells. HPLC–UV analysis revealed a remarkable 33-fold higher fucoxanthin content in CM compared to the ethanol extract. GC–MS analysis identified elevated levels of cholest-5-en-3-ol, phytol, eicosapentaenoic acid methyl ester, methyl palmitate, palmitoleic acid methyl ester, and neophytadiene in the CM extract. These findings suggest that <i>Odontella aurita</i> CM extract is a promising antioxidant candidate for preventing or treating inflammatory diseases, consequently emphasizing its potential for further development.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00898-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140924791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The potential modulatory impact of garlic-selenium nanoparticles coated with synthetic tocopherol polyethylene glycol-succinate against lead acetate toxicity in male rabbits","authors":"Mohamed Abdel Rida Yaseen,&nbsp;Madiha Hadj Ayed,&nbsp;Jabbar A. A. Al-Saaidi","doi":"10.1186/s13765-024-00893-8","DOIUrl":"10.1186/s13765-024-00893-8","url":null,"abstract":"<div><p>Toxic heavy metal lead enters in the environment due to industrial and anthropogenic activity threatens ecosystems and public health. Natural garlic extract (GE) exhibits antioxidant properties and various applications against several ailments. Therefore, this study scrutinized the protective effects of tocopherol polyethylene glycol succinate-coated garlic selenium (TPGS-GSNP) against lead acetate (LA) toxicity in rabbits. Sixty-four mature male rabbits were involved and divided into 8 equal groups. They received distilled water (negative control; T1), 30 mg/kg bw of LA (positive control; T2), 800 mg/kg bw of GE (T3), GE + LA (T4), 1 mg/kg bw of TPGS-Selenium (T5), TPGS-S + LA (T6), 1 mg/kg bw of TPGS-GSNP (T7), and TPGS-GSNP + LA (T8). Consequently, treatments were administered three times a week for 12 weeks. Following the treatment period, serum oxidant-antioxidant, protein, and lipid profiles, liver and kidney function, histopathological findings of the adrenal, liver, and kidneys, femur bone marrow chromosomal aberrations, and mitotic activity were collected and analysed. LA exposure showed significant reductions in antioxidant levels, organ weights, and mitotic activity while increasing oxidative stress, corticosteroid levels, and chromosomal aberrations. Importantly, TPGS-GSNP administration significantly improved these markers compared to the LA group. In addition, histological analysis revealed structural improvements of the studied organs in the TPGS-GSNP group compared to the LA group, which displayed high cellular necrotic and degenerative changes. In conclusion, synthetic TPGS-GSNP demonstrated higher protective efficacy against LA-induced toxicity compared to natural GE or selenium alone. However, more future studies could be conducted to explore the potential of TPGS-GSNP as an anticancer or immunomodulatory agent.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00893-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140919208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of rice hull biochar treatment on net ecosystem carbon budget and greenhouse gas emissions in Chinese cabbage cultivation on infertile soil","authors":"Do-Gyun Park,&nbsp;Hyeon-Cheol Jeong,&nbsp;Eun-Bin Jang,&nbsp;Jong-Mun Lee,&nbsp;Hyoung-Seok Lee,&nbsp;Hye-Ran Park,&nbsp;Sun-Il Lee,&nbsp;Do-Gyun Park,&nbsp;Eun-Bin Jang,&nbsp;Taek-Keun Oh","doi":"10.1186/s13765-024-00903-9","DOIUrl":"10.1186/s13765-024-00903-9","url":null,"abstract":"<div><p>Biochar, with its potential to enhance soil fertility, sequester carbon, boost crop yields and reduce greenhouse gas emissions, offers a solution. Addressing the challenges posed by climate change is crucial for food security and agriculture. However, its widespread adoption in agriculture remains in its infancy. This study assessed the effects of rice hull biochar on cabbage production and greenhouse gas emissions, especially nitrous oxide (N₂O). A trial, employing a randomized block design in triplicate was conducted from September 13 to November 23, 2022, where \"Cheongomabi\" cabbage was cultivated with N-P₂O₅-K₂O fertilization at 32<span>(-)</span>7.8<span>(-)</span>19.8 kg 10a⁻¹. Additional fertilizer was applied twice post-sowing. The Biochar application rates were control = 0 ton ha⁻¹, B1 = 1 ton ha⁻¹, B3 = 3 ton ha⁻¹, and B5 = 5 ton ha⁻¹. The aboveground biomass of autumn cabbage harvested 82 days after sowing was 2.40–2.70 kg plant⁻¹ in the control and biochar treatments (B1, B3, and B5), with no significant differences (<i>p</i> &gt; 0.05). Cumulative CO₂ emissions during cultivation varied across treatment groups, with initial and cumulative emissions of 10.40–17.94 g m⁻² day⁻¹ and 3.63–4.43 ton ha⁻¹, respectively. N₂O emissions decreased with higher biochar application: reductions of 2.9%, 25.4%, and 41.1% in the B1, B3, and B5 treatments, respectively, compared to the control. The biochar application had no significant impact on yield but curbed soil emissions, Net ecosystem carbon balance during cabbage cultivation ranged from 0.42 to 3.41 ton ha⁻¹ for the B1, B3, and B5 treatments, respectively, compared to control. Overall, the study underscores biochar’s role in mitigating emissions and boosting soil carbon during cabbage cultivation in fall.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00903-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140914583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights into the abundance, expression and diversity of key denitrification genes in an ecologically managed greenhouse agricultural soil","authors":"R. Hernández Maqueda,&nbsp;I. Ballesteros,&nbsp;D. Meca,&nbsp;R. Linacero,&nbsp;F. del Moral","doi":"10.1186/s13765-024-00901-x","DOIUrl":"10.1186/s13765-024-00901-x","url":null,"abstract":"<div><p>Understanding the bacteria associated with nitrification and denitrification is crucial for comprehending the processes that lead to nitrous oxide emissions in agricultural greenhouse soils. Therefore, it is important to determine their abundance and expression to gain insight into these processes.</p><p>The aim of this study was to explore the bacterial communities associated with denitrification in a greenhouse agricultural soil amended with crop residues and manure for six years. For this purpose, we proceeded to detect and quantify the genes <i>nirK</i> and <i>nirS</i> and the gene <i>nosZ</i> through clone library construction, sequencing, phylogenetic analysis, and quantitative polymerase chain reaction (qPCR). Sequence analysis based on the clone library revealed that many of the <i>nirS</i> or <i>nirK</i> genes detected were not closely related to known denitrifier bacteria, but some of the <i>nosZ</i> sequences were related to the genera such as <i>Pseudomonas</i>, <i>Halomonas</i>, and <i>Marinobacter</i>. Furthermore, the qPCR revealed a high abundance of DNA copies in <i>nirK</i>, 6.08 × 10⁹ ± 1.16 × 10⁹, while <i>nirS</i> and <i>nosZ</i> showed lower values, 9.05 × 10⁶ ± 1.65 × 10⁶ and 8.71 × 10⁶ ± 1.44 × 10⁶, respectively. However, the highest expression rate was observed for <i>nirS</i> (mRNA/DNA ratio = 3.10 × 10^− 3), while <i>nirK</i> and <i>nosZ</i> showed 10-fold lower expression rates (4.4 × 10^− 4 and 3.5 × 10^− 4, respectively). The results of this work provide a preliminary overview of the diversity, abundance and expression of key genes associated with the denitrification process in this type of soil and are a starting point for further studies to understand how this type of soil management can influence the denitrification process.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00901-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140826082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Application of CRISPR-Based C-to-G base editing in rice protoplasts","authors":"Jimin Lee,&nbsp;Nuri Oh,&nbsp;Jae‑Young Yun,&nbsp;Hee Soon Choi,&nbsp;Jang‑Kyun Seo,&nbsp;Jin‑Ho Kang,&nbsp;Choonkyun Jung","doi":"10.1186/s13765-024-00895-6","DOIUrl":"10.1186/s13765-024-00895-6","url":null,"abstract":"","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00895-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140641693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hyperoside ameliorates depression-like behavior in ovariectomized mice","authors":"Kyung Hee Hong,&nbsp;Jonghoon Jung,&nbsp;Minji Kim,&nbsp;Min Young Um","doi":"10.1186/s13765-024-00897-4","DOIUrl":"10.1186/s13765-024-00897-4","url":null,"abstract":"<div><p>The physiological changes caused by the decline in estrogen levels due to menopause are linked to an increased risk of depression. This study investigated the antidepressant effects of hyperoside (HYP), a natural flavonol glycoside, and its associated molecular mechanisms in primary hippocampal neurons and ovariectomized (OVX) mice. HYP treatment increased nitric oxide (NO) production and neuronal nitric oxide synthase (nNOS) expression in primary hippocampal neurons; additionally, it upregulated the expression of brain-derived neurotrophic factor (BDNF) and phosphorylated tropomyosin receptor kinase B (TrkB). In OVX mice, HYP treatment significantly improved depression-like behaviors in an open field test to a level comparable to estrogen treatment. Furthermore, HYP treatment upregulated OVX-induced decreased nNOS expression and BDNF-TrkB signaling in the hippocampus. Therefore, this study suggests that HYP exhibits antidepressant potential by addressing estrogen deficiency-induced alterations, specifically by restoring nNOS expression, promoting NO production, and concurrently enhancing BDNF-TrkB signaling in OVX mice.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00897-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140621284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of metabolites and in vitro hypoglycemic activity of Taiwanofungus camphoratus cultured using various methods","authors":"YongFei Ming,&nbsp;Yin Li,&nbsp;JianZhi Chu,&nbsp;XiaoShuang Zhou,&nbsp;YuXuan Huang,&nbsp;ShuDe Yang,&nbsp;YueJun Mu,&nbsp;Lin Wang,&nbsp;Rui Zhang,&nbsp;XianHao Cheng","doi":"10.1186/s13765-024-00890-x","DOIUrl":"10.1186/s13765-024-00890-x","url":null,"abstract":"<div><p><i>Taiwanofungus camphoratus</i> has attracted much attention because it can abundantly produce various active substances that exhibit blood-sugar lowering, immunity improving, and antioxidant properties. Currently, <i>T. camphoratus</i> is cultured using four main methods: cutting wood culture, solid-state fermentation, submerged fermentation, and dish culture. <i>T. camphoratus</i> produces different metabolites under different culture methods. In this study, nontargeted metabolomics was used to compare the metabolites of <i>T. camphoratus</i> produced under these four culture methods. Principal component analysis and supervised partial least squares-discriminant analysis were used to analyze the differences in the metabolites. Moreover, in vitro hypoglycemic activity of <i>T. camphoratus</i> extracts produced under four culture methods was compared by assessing their ability to inhibit the activity of α-glucosidase, α-amylase, and sucrase. A total of 186 metabolites were identified. In total, 127 metabolites were common under the four culture methods. Under solid-state fermentation, submerged fermentation, and cutting wood culture, 12, 1, and 4 metabolites were unique, respectively. The differential metabolites produced by <i>T. camphoratus</i> under four culture methods were mainly triterpenoids, phenolic compounds, and fatty acid compounds. α-glucosidase, α-amylase, and sucrase activity inhibition was the best using <i>T. camphoratus</i> extract obtained under cutting wood culture; the inhibition rates were 55.97%, 51.96%, and 78.02%, respectively, which were comparable to those exhibited by 0.001, 3, and 12 mg/mL acarbose (positive control). The metabolites produced by <i>T. camphoratus</i> and α-glucosidase, α-amylase, and sucrase inhibitory activities were different under the four culture methods. Cutting wood culture exhibited the best enzyme inhibitory activity. This study provided a theoretical basis for further use and development of various culture methods for the rational production of active metabolites of <i>T. camphoratus</i>.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00890-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140537489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}