Applied Biological Chemistry最新文献

{"title":"Insights into the abundance, expression and diversity of key denitrification genes in an ecologically managed greenhouse agricultural soil","authors":"R. Hernández Maqueda,&nbsp;I. Ballesteros,&nbsp;D. Meca,&nbsp;R. Linacero,&nbsp;F. del Moral","doi":"10.1186/s13765-024-00901-x","DOIUrl":"10.1186/s13765-024-00901-x","url":null,"abstract":"<div><p>Understanding the bacteria associated with nitrification and denitrification is crucial for comprehending the processes that lead to nitrous oxide emissions in agricultural greenhouse soils. Therefore, it is important to determine their abundance and expression to gain insight into these processes.</p><p>The aim of this study was to explore the bacterial communities associated with denitrification in a greenhouse agricultural soil amended with crop residues and manure for six years. For this purpose, we proceeded to detect and quantify the genes <i>nirK</i> and <i>nirS</i> and the gene <i>nosZ</i> through clone library construction, sequencing, phylogenetic analysis, and quantitative polymerase chain reaction (qPCR). Sequence analysis based on the clone library revealed that many of the <i>nirS</i> or <i>nirK</i> genes detected were not closely related to known denitrifier bacteria, but some of the <i>nosZ</i> sequences were related to the genera such as <i>Pseudomonas</i>, <i>Halomonas</i>, and <i>Marinobacter</i>. Furthermore, the qPCR revealed a high abundance of DNA copies in <i>nirK</i>, 6.08 × 10⁹ ± 1.16 × 10⁹, while <i>nirS</i> and <i>nosZ</i> showed lower values, 9.05 × 10⁶ ± 1.65 × 10⁶ and 8.71 × 10⁶ ± 1.44 × 10⁶, respectively. However, the highest expression rate was observed for <i>nirS</i> (mRNA/DNA ratio = 3.10 × 10^− 3), while <i>nirK</i> and <i>nosZ</i> showed 10-fold lower expression rates (4.4 × 10^− 4 and 3.5 × 10^− 4, respectively). The results of this work provide a preliminary overview of the diversity, abundance and expression of key genes associated with the denitrification process in this type of soil and are a starting point for further studies to understand how this type of soil management can influence the denitrification process.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00901-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140826082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Application of CRISPR-Based C-to-G base editing in rice protoplasts","authors":"Jimin Lee,&nbsp;Nuri Oh,&nbsp;Jae‑Young Yun,&nbsp;Hee Soon Choi,&nbsp;Jang‑Kyun Seo,&nbsp;Jin‑Ho Kang,&nbsp;Choonkyun Jung","doi":"10.1186/s13765-024-00895-6","DOIUrl":"10.1186/s13765-024-00895-6","url":null,"abstract":"","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00895-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140641693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hyperoside ameliorates depression-like behavior in ovariectomized mice","authors":"Kyung Hee Hong,&nbsp;Jonghoon Jung,&nbsp;Minji Kim,&nbsp;Min Young Um","doi":"10.1186/s13765-024-00897-4","DOIUrl":"10.1186/s13765-024-00897-4","url":null,"abstract":"<div><p>The physiological changes caused by the decline in estrogen levels due to menopause are linked to an increased risk of depression. This study investigated the antidepressant effects of hyperoside (HYP), a natural flavonol glycoside, and its associated molecular mechanisms in primary hippocampal neurons and ovariectomized (OVX) mice. HYP treatment increased nitric oxide (NO) production and neuronal nitric oxide synthase (nNOS) expression in primary hippocampal neurons; additionally, it upregulated the expression of brain-derived neurotrophic factor (BDNF) and phosphorylated tropomyosin receptor kinase B (TrkB). In OVX mice, HYP treatment significantly improved depression-like behaviors in an open field test to a level comparable to estrogen treatment. Furthermore, HYP treatment upregulated OVX-induced decreased nNOS expression and BDNF-TrkB signaling in the hippocampus. Therefore, this study suggests that HYP exhibits antidepressant potential by addressing estrogen deficiency-induced alterations, specifically by restoring nNOS expression, promoting NO production, and concurrently enhancing BDNF-TrkB signaling in OVX mice.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00897-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140621284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of metabolites and in vitro hypoglycemic activity of Taiwanofungus camphoratus cultured using various methods","authors":"YongFei Ming,&nbsp;Yin Li,&nbsp;JianZhi Chu,&nbsp;XiaoShuang Zhou,&nbsp;YuXuan Huang,&nbsp;ShuDe Yang,&nbsp;YueJun Mu,&nbsp;Lin Wang,&nbsp;Rui Zhang,&nbsp;XianHao Cheng","doi":"10.1186/s13765-024-00890-x","DOIUrl":"10.1186/s13765-024-00890-x","url":null,"abstract":"<div><p><i>Taiwanofungus camphoratus</i> has attracted much attention because it can abundantly produce various active substances that exhibit blood-sugar lowering, immunity improving, and antioxidant properties. Currently, <i>T. camphoratus</i> is cultured using four main methods: cutting wood culture, solid-state fermentation, submerged fermentation, and dish culture. <i>T. camphoratus</i> produces different metabolites under different culture methods. In this study, nontargeted metabolomics was used to compare the metabolites of <i>T. camphoratus</i> produced under these four culture methods. Principal component analysis and supervised partial least squares-discriminant analysis were used to analyze the differences in the metabolites. Moreover, in vitro hypoglycemic activity of <i>T. camphoratus</i> extracts produced under four culture methods was compared by assessing their ability to inhibit the activity of α-glucosidase, α-amylase, and sucrase. A total of 186 metabolites were identified. In total, 127 metabolites were common under the four culture methods. Under solid-state fermentation, submerged fermentation, and cutting wood culture, 12, 1, and 4 metabolites were unique, respectively. The differential metabolites produced by <i>T. camphoratus</i> under four culture methods were mainly triterpenoids, phenolic compounds, and fatty acid compounds. α-glucosidase, α-amylase, and sucrase activity inhibition was the best using <i>T. camphoratus</i> extract obtained under cutting wood culture; the inhibition rates were 55.97%, 51.96%, and 78.02%, respectively, which were comparable to those exhibited by 0.001, 3, and 12 mg/mL acarbose (positive control). The metabolites produced by <i>T. camphoratus</i> and α-glucosidase, α-amylase, and sucrase inhibitory activities were different under the four culture methods. Cutting wood culture exhibited the best enzyme inhibitory activity. This study provided a theoretical basis for further use and development of various culture methods for the rational production of active metabolites of <i>T. camphoratus</i>.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00890-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140537489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Piperine reduces hair oiliness by inhibiting adipogenesis of hair stem cells","authors":"Minyoung Im,&nbsp;Nackhyoung Kim,&nbsp;Ui-Hyun Park,&nbsp;Hyeon Ho Heo,&nbsp;Soo-Jong Um","doi":"10.1186/s13765-024-00889-4","DOIUrl":"10.1186/s13765-024-00889-4","url":null,"abstract":"<div><p>Piperine, an alkaloid compound in black pepper (<i>Piper nigrum</i>), has beneficial bioactivities. Specifically, piperine inhibits adipogenesis in 3T3-L1 cells by suppressing the transcriptional activity of PPARγ. Control of hair oiliness, which is related to adipogenic regulation, is important to prevent hair loss. Excessive sebum from the sebaceous gland (SG) can cause acne, folliculitis, or irritated skin by clogging pores. To investigate the in vivo function of piperine in SG, we used mice fed a high-fat diet (HFD). The HFD increased the size and Oil Red O (ORO) staining intensity of SG, which were significantly reduced by piperine. The HFD also upregulated the expression of sebocyte-associated genes, including PPARγ target genes, an effect reversed by piperine. In CD34/CD49f double-positive hair follicle bulge stem cells isolated from mouse vibrissae, piperine inhibited cellular adipogenesis, likely via transcriptional repression of related genes. Furthermore, piperine reduced the thickness of subcutaneous fat. In human dermal papilla cells, piperine inhibited cellular adipogenesis, as shown by the reduction in ORO staining and the downregulation of PPARγ target genes. In conclusion, piperine can be used to reduce hair greasiness by suppressing adipogenesis in hair stem cells.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00889-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140340212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of safety and efficacy of febantel and fenbendazole in fish and exposure assessment","authors":"Min Ji Kim,&nbsp;Ji Young Kim,&nbsp;Dong Woo Shin,&nbsp;Hyun-Kyung Kim","doi":"10.1186/s13765-024-00879-6","DOIUrl":"10.1186/s13765-024-00879-6","url":null,"abstract":"<div><p>Fish are susceptible to blood-sucking parasite infections, which cause severe anemia, dyspnea, and ultimately death. However, veterinary drugs available for fish to treat such infectious diseases are lacking; thus, livestock drugs have been repurposed as aquatic animal drugs. Febantel (FBT) and fenbendazole (FBZ) are representative antiparasitic agents for livestock such as cattle, swine, and poultry, and are considered suitable as aquatic animal drugs. Therefore, we investigated the safety and efficacy of FBT and FBZ in fish and performed a risk assessment to determine the maximum residue limit in fish. Most studies indicate that FBT is rapidly converted to FBZ, which is metabolized to oxfendazole and oxfendazole sulfone. FBZ was frequently detectable in the plasma and tissues (e.g., muscle, skin, and the liver) in significant quantities than other metabolites. We regarded the liver as the target organ because reversible hepatocytic changes were observed in fish after administration of 100 mg/kg FBT for 9 days. No toxicological effects, such as increased mortality or decreased appetite, were observed when the fish were administered 50 mg/kg FBT for 3 days. The efficacy of the drugs was verified in various parasites, including <i>H. heterocerca, H. okamotoi or Z. japonica</i>, and <i>M. seriolae</i>, as causative agents of beko disease through laboratory and field trials. Although toxicity studies on FBZ in fish are limited, its safety has been demonstrated from toxicity studies in a wide range of animal models. The risk from using FBT and FBZ was negligible for human health because the ratio of the estimates of dietary exposure and acceptable daily intake was 78.4%.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00879-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140328974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nutrient recycling of source-separated human faeces using biochar immobilized indigenous psychrotrophic bacteria for sustaining the agroecosystems of north-western Himalaya","authors":"Shruti Sinai Borker,&nbsp;Aman Thakur,&nbsp;Krishna Kanta Pandey,&nbsp;Pallavi Sharma,&nbsp;Vivek Manyapu,&nbsp;Abhishek Khatri,&nbsp;Rakshak Kumar","doi":"10.1186/s13765-024-00887-6","DOIUrl":"10.1186/s13765-024-00887-6","url":null,"abstract":"<div><p>The Himalayan composting toilets (CTs) offer a sustainable solution for converting human faeces (HF) into compost, supplementing the low-fertile land of the region. However, CTs face challenges such as delayed composting processes (6–8 months), increased heavy metal content, and foul odour. Therefore, the current study evaluated biochar-amended psychrotrophic bacteria for HF degradation under low-temperature conditions (10 ± 2 °C). Out of 153 psychrotrophic bacteria isolated from HF compost, 17 bacterial strains were selected based on highest and two or more hydrolytic activities. Furthermore, considering the isolation source, bacterial strains were examined for haemolytic activity, biofilm formation, cytotoxicity and seed germination assay. In total, 14 potential strains belonging to <i>Pseudomonas</i>, <i>Microbacterium</i>, <i>Arthrobacter</i>, <i>Streptomyces</i>, <i>Glutamicibacter</i>, <i>Rhodococcus</i>, <i>Serratia</i>, <i>Exiguobacterium</i>, and <i>Jeotgalicoccus</i> genera were considered safe for both human handling and plants. The composting process was conducted in modified plastic drums at 10 ± 2 °C for 90 days through two treatments: Treatment 1 (T1) involving HF, non-immobilized biochar and cocopeat, and Treatment 2 (T2) involving HF, consortium-immobilized biochar and cocopeat. The consortium-immobilized biochar (T2) degraded HF within 90 days with hemicellulose and cellulose degradation ratios of 73.9% and 62.4%, respectively (p ≤ 0.05). The compost maturation indices like C/N ratio (16.5 ± 1.85), total nitrogen (2.66 ± 0.07), total phosphate (0.4 ± 0.005), total potassium (1.8 ± 0.05) also improved in T2 treatment (p ≤ 0.05). Additionally, T2 was more effective in achieving safe levels of faecal coliforms (&lt; 1000 MPN g⁻¹) and reducing heavy metal content compared to T1. 16S rRNA amplicon-based analysis demonstrated an enhancement of bacterial community diversity in T2, with the presence of <i>Rhodococcus</i>, <i>Pseudomonas</i>, <i>Arthrobacter</i>, and <i>Streptomyces</i> at the end of the composting period promoting HF degradation. Furthermore, T2-fertilized soil showed a germination index (121 ± 0.4, p ≤ 0.05) and stimulated root, shoot and yield by 110%, 45.2%, and 288%, respectively, in pea (<i>Pisum sativum</i> var. AS-10) compared to T1 (49.6%, 19%, and 5.8%, respectively) (p ≤ 0.05). In conclusion, the developed biochar-based formulation proved effective in degrading HF at low temperatures, mitigating foul odours, reducing heavy metals, and enhancing the agronomic value of the final compost. This study presents a promising approach for the sustainable management of HF that can supplement the non-nutritive soil of high-altitude regions.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00887-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140328976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of anti-obesity effects of green, fermented, and γ-aminobutyric acid teas in a high-fat diet-induced mouse model","authors":"Seung-Jun Lee,&nbsp;Kyoung Hwan Cho,&nbsp;Jong Cheol Kim,&nbsp;Ho Jin Choo,&nbsp;Jeong-Yun Hwang,&nbsp;Hyun Chin Cho,&nbsp;Young-Sool Hah","doi":"10.1186/s13765-024-00888-5","DOIUrl":"10.1186/s13765-024-00888-5","url":null,"abstract":"<div><p>Obesity, a prevalent disease associated with numerous chronic conditions, including hyperlipidemia, hyperglycemia, diabetes, and metabolic syndrome, remains a major global health challenge. This study investigated the potential of green tea (GT), fermented tea (FT), and γ-aminobutyric acid (GABA) tea (GBT), which are rich in phytonutrients and polyphenols, for the management of obesity. Using a high-fat diet-induced obese mouse model (C57BL/6N), we explored the effect of these teas on various obesity-related parameters. The mice were categorized into five groups: normal diet with water, high-fat diet with water, and high-fat diet supplemented with GT, FT, or GBT. Over 13 weeks, we monitored body weight, perirenal and liver fat, adipocyte lipid accumulation, and key metabolic indicators, such as serum cholesterol, leptin, insulin, and fasting blood glucose. These teas contain beneficial phytochemicals such as GABA, theanine, and caffeine, and have demonstrated an enhanced antioxidant capacity, which increases the scavenging of free radicals and may reduce oxidative stress. The animal study indicated a decrease in feeding efficiency and significant reductions in body weight liver fat, epididymal fat, and perirenal fat, as well as in adipocyte lipid accumulation. Additionally, notable improvements were observed in metabolic health indicators, including reductions in serum cholesterol, leptin, insulin, and fasting blood glucose levels. Our findings revealed that GT, FT, or GBT significantly counteracted the negative effects of a high-fat diet, suggesting their potential in combating obesity and related metabolic disorders.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00888-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140297156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Murraya koenigii (L.) Sprengel seeds and pericarps in relation to their chemical profiles: new approach for multidrug resistant Acinetobacter baumannii ventilator-associated pneumonia","authors":"Riham A. El-Shiekh,&nbsp;Rana Elshimy,&nbsp;Asmaa A. Mandour,&nbsp;Hanaa A. H. Kassem,&nbsp;Amal E. Khaleel,&nbsp;Saleh Alseekh,&nbsp;Alisdair R. Fernie,&nbsp;Mohamed A. Salem","doi":"10.1186/s13765-024-00886-7","DOIUrl":"10.1186/s13765-024-00886-7","url":null,"abstract":"<div><p><i>Acinetobacter</i> <i>baumannii</i> is without a doubt one of the most problematic bacteria causing hospital-acquired nosocomial infections in today's healthcare system. To solve the high prevalence of multi-drug resistant (MDR) in <i>A.</i> <i>baumannii</i>, we investigated one of the medicinal plants traditionally used as antibacterial agent; namely <i>Murraya</i> <i>koenigii</i> (L.) Sprengel. The total methanolic extracts of seeds and pericarps were prepared and their anti-bacterial activity was assessed using the agar diffusion method and minimum inhibitory concentration (MIC) was then calculated as compared to tigecycline. Then, an in-vivo murine model was established which confirmed the promising activity of <i>M.</i> <i>koenigii</i> seeds in demonstrating anti-bacterial and anti-inflammatory actions. The histopathological study of lungs, scoring of pulmonary lesions, counting of bacterial loads after infection by multi-drug resistant <i>A.</i> <i>baumannii</i> all provided evidence to support these findings. LC–MS/MS profiling coupled to molecular networking and chemometrics detected the presence of carbazole alkaloids, and coumarins as dominate metabolites of the active seed extracts. Positively correlated metabolites to antibacterial potential were 6-(2ʹ,3ʹ-dihydroxy-3-methylbutyl)-8-prenylumbelliferone, scopoline, and 5-methoxymurrayatin. An in-silico study was also performed on the crystal structure of MurF from <i>A.</i> <i>baumannii</i> (PDB ID: 4QF5), the studied structures of the mentioned extracts revealed good docking interaction at the active site suggestive of competition with the ATP ligand. These collective findings suggest that extracts of <i>Murraya</i> <i>koenigii</i> (L.) Sprengel seed is a novel prospective for the discovery of drug candidates against infections caused by MDR <i>A.</i> <i>baumannii</i>.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00886-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140291351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and application of a high-performance liquid chromatography diode-array detection (HPLC–DAD) method for the simultaneous quantification of phenolic compounds in the aerial part of Glehnia littoralis","authors":"Yun Ji Park,&nbsp;Yeong Bin Choi,&nbsp;Sang-Bin Oh,&nbsp;Jinyoung Moon,&nbsp;To Quyen Truong,&nbsp;Phuong Kim Huynh,&nbsp;Sang Min Kim","doi":"10.1186/s13765-024-00884-9","DOIUrl":"10.1186/s13765-024-00884-9","url":null,"abstract":"<div><p><i>Glehnia littoralis</i>, a medicinal herb employed in traditional practices for alleviating fatigue, cough, and a dry throat, is recognized for its beneficial properties due to a diverse array of active compounds found in its extracts. For example, the <i>G. littoralis</i> roots (Radix Glehniae) mainly contain coumarins and phenolic acids, serving as the primary focus of this study. Despite the widespread use of the tools in various industries and the development of multiple analytical methods for their examination, the edible aerial parts have industrial potential, and there is currently no analytical method available to identify their key components. In this study, a high-performance liquid chromatography method combined with diode array detection (HPLC–DAD) was developed to simultaneously detect 16 phenolic compounds previously reported to be present in the edible aerial parts of <i>G. littoralis</i>. The proposed approach included using gradient elution to change the solvent system from water/acetonitrile to water/methanol. Furthermore, the method validation was conducted, assessing its linearity, limit of detection, limit of quantification, precision, accuracy, and recovery, all of which demonstrated satisfactory results. Subsequently, the developed method was applied to quantify the phenolic compounds in various <i>G. littoralis</i> samples obtained from different organs, solvent extraction processes, and processing methods. Moreover, the online HPLC-ABTS (2,2ʹ-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) assay was used to evaluate the antioxidant capacities of individual constituents, identifying four important antioxidants and estimate the overall antioxidant capacity of the <i>G. littoralis</i> extract.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00884-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140188472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}