Anticancer effects of aloe-emodin from Rheum undulatum L. through activation of the p53 pathway in human prostate cancer cells

IF 2.3 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY
Nguyen Khoi Song Tran, Nhu Quynh Nguyen, Sullim Lee, Seung Hyun Kim, Daesik Jeong, Eunjeong Seo, Jin Ju Park, Jaejin Cho, Ki Sung Kang
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引用次数: 0

Abstract

Aloe-emodin, an anthraquinone compound naturally derived from Rheum undulatum L., has gained extensive research attention owing to its various pharmacological effects, including its potential as an anticancer, antivirus, anti-inflammatory, antibacterial, and anti-parasitic agent. It has demonstrated notable inhibitory effects against various types of cancer and cancer cells. Prostate cancer is among the most commonly identified cancers globally and remains a leading cause of cancer-associated deaths in men, often presenting challenges in early detection due to its asymptomatic nature during initial stages. The aim of present study was to determine the biological activity of aloe-emodin obtained from Rheum undulatum L. involving activation of the p53-dependent pathway in certain human prostate cancer cell lines. We explored the mechanisms underlying the anticancer effects of aloe-emodin using LNCaP cells, which include p53-wild type and phosphatase and tensin homolog-deficient mutated genes, a widely studied model in genomic research. Aloe-emodin induced apoptosis in LNCaP cells through several mechanisms, including upregulation of the cleavage of caspase-8 (a cross-linked promoter of cell death signals), phosphorylation of p53 at serine 15, DNA fragmentation, cleavage of poly [ADP-ribose] polymerase, and promotion of cell death. These findings strongly indicated that aloe-emodin's anticancer properties in human prostate cancer involve the activation of p53-induced cellular senescence. Conclusively, the findings of this study imply that aloe-emodin extracted from Rheum undulatum L. is a potential therapeutic compound for adjuvant chemotherapy that induces apoptosis and pyroptosis, an innate immune response, in preventing the progression of precancerous lesions in patients with prostate cancer.

大黄芦荟大黄素通过激活人前列腺癌细胞p53通路的抗癌作用
芦荟大黄素是一种天然从大黄中提取的蒽醌类化合物,因其具有抗癌、抗病毒、抗炎、抗菌和抗寄生虫等多种药理作用而受到广泛关注。对多种类型的肿瘤和癌细胞具有显著的抑制作用。前列腺癌是全球最常见的癌症之一,仍然是男性癌症相关死亡的主要原因,由于其在初始阶段无症状,往往在早期发现方面面临挑战。本研究的目的是确定从大黄中提取的芦荟大黄素在某些人前列腺癌细胞中激活p53依赖通路的生物学活性。我们利用LNCaP细胞探索了芦荟大黄素抗癌作用的机制,LNCaP细胞包括p53野生型和磷酸酶和紧张素同源缺陷突变基因,这是基因组研究中广泛研究的模型。芦荟大黄素通过多种机制诱导LNCaP细胞凋亡,包括上调caspase-8(细胞死亡信号的交联启动子)的裂解、p53丝氨酸15位点的磷酸化、DNA断裂、聚[adp核糖]聚合酶的裂解和促进细胞死亡。这些发现有力地表明,芦荟大黄素在人类前列腺癌中的抗癌特性涉及激活p53诱导的细胞衰老。总之,本研究结果表明,从大黄中提取的芦荟大黄素是一种潜在的辅助化疗治疗化合物,可诱导细胞凋亡和焦亡,这是一种先天免疫反应,可预防前列腺癌患者癌前病变的进展。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Applied Biological Chemistry
Applied Biological Chemistry Chemistry-Organic Chemistry
CiteScore
5.40
自引率
6.20%
发文量
70
审稿时长
20 weeks
期刊介绍: Applied Biological Chemistry aims to promote the interchange and dissemination of scientific data among researchers in the field of agricultural and biological chemistry. The journal covers biochemistry and molecular biology, medical and biomaterial science, food science, and environmental science as applied to multidisciplinary agriculture.
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