Applied Biological Chemistry最新文献

{"title":"Bioactive constituents from Carica papaya fruit: implications for drug discovery and pharmacological applications","authors":"Hiruni Sandunika kumarasinghe,&nbsp;Ji-Hyang Kim,&nbsp;Su-Lim Kim,&nbsp;Kyeoung Cheol Kim,&nbsp;Rambukkana Maggonage Thiruni Dananjana Perera,&nbsp;Seong-Cheol Kim,&nbsp;Dong-Sun Lee","doi":"10.1186/s13765-024-00962-y","DOIUrl":"10.1186/s13765-024-00962-y","url":null,"abstract":"<div><p><i>Carica papaya</i>, commonly known as papaya, is a fruit recognized for its substantial medicinal potential, primarily due to its wide range of bioactive compounds. This review thoroughly examines the pharmacological implications of these constituents and highlights their potential applications in drug discovery and therapy. Papaya is abundant in vitamins (A, C, and E), minerals, enzymes, and phytochemicals such as flavonoids, phenolic acids, carotenoids, and alkaloids, all of which contribute to its antioxidant, anti-aging, anti-inflammatory, and anticancer effects. The fruit demonstrates significant activity against diabetes, obesity, cardiovascular diseases, and gastrointestinal disorders. This review also discusses how environmental factors, including temperature, light, soil quality, and rainfall, impact the phytochemical composition of papaya, thereby influencing its medicinal properties. Both in vitro and in vivo studies have highlighted the therapeutic potential of papaya-derived compounds in various health conditions, including cancer, diabetes, wound healing, and cardiovascular health. Additionally, we explore papaya's role in promoting gut health and its antimicrobial properties against bacterial and viral pathogens. In conclusion, the diverse pharmacological activities of papaya's bioactive compounds position it as a valuable candidate for further research and development in drug discovery and therapeutic applications.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00962-y","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142811100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anticancer effects of aloe-emodin from Rheum undulatum L. through activation of the p53 pathway in human prostate cancer cells","authors":"Nguyen Khoi Song Tran,&nbsp;Nhu Quynh Nguyen,&nbsp;Sullim Lee,&nbsp;Seung Hyun Kim,&nbsp;Daesik Jeong,&nbsp;Eunjeong Seo,&nbsp;Jin Ju Park,&nbsp;Jaejin Cho,&nbsp;Ki Sung Kang","doi":"10.1186/s13765-024-00956-w","DOIUrl":"10.1186/s13765-024-00956-w","url":null,"abstract":"<div><p>Aloe-emodin, an anthraquinone compound naturally derived from <i>Rheum undulatum</i> L., has gained extensive research attention owing to its various pharmacological effects, including its potential as an anticancer, antivirus, anti-inflammatory, antibacterial, and anti-parasitic agent. It has demonstrated notable inhibitory effects against various types of cancer and cancer cells. Prostate cancer is among the most commonly identified cancers globally and remains a leading cause of cancer-associated deaths in men, often presenting challenges in early detection due to its asymptomatic nature during initial stages. The aim of present study was to determine the biological activity of aloe-emodin obtained from <i>Rheum undulatum</i> L. involving activation of the p53-dependent pathway in certain human prostate cancer cell lines. We explored the mechanisms underlying the anticancer effects of aloe-emodin using LNCaP cells, which include p53-wild type and phosphatase and tensin homolog-deficient mutated genes, a widely studied model in genomic research. Aloe-emodin induced apoptosis in LNCaP cells through several mechanisms, including upregulation of the cleavage of caspase-8 (a cross-linked promoter of cell death signals), phosphorylation of p53 at serine 15, DNA fragmentation, cleavage of poly [ADP-ribose] polymerase, and promotion of cell death. These findings strongly indicated that aloe-emodin's anticancer properties in human prostate cancer involve the activation of p53-induced cellular senescence. Conclusively, the findings of this study imply that aloe-emodin extracted from <i>Rheum undulatum</i> L<i>.</i> is a potential therapeutic compound for adjuvant chemotherapy that induces apoptosis and pyroptosis, an innate immune response, in preventing the progression of precancerous lesions in patients with prostate cancer.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00956-w","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142810879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of the safety and hepatic lipid-lowering effects of Lactobacillus delbrueckii subsp. lactis CKDB001","authors":"Hyunchae Joung,&nbsp;Jaeryang Chu,&nbsp;Yoo Jin Kwon,&nbsp;Kyung Hwan Kim,&nbsp;Chang Hun Shin,&nbsp;Jung-Heun Ha","doi":"10.1186/s13765-024-00949-9","DOIUrl":"10.1186/s13765-024-00949-9","url":null,"abstract":"<div><p>Probiotics have been shown to provide health benefits for several metabolic diseases, including obesity, type 2 diabetes, and metabolic dysfunction-associated steatotic liver disease (MASLD), by modulating the gut microbiota. In this study, we evaluated the safety and efficacy of <i>Lactobacillus delbrueckii</i> subsp. <i>lactis</i> CKDB001 as a potential therapeutic candidate for the treatment of MASLD. We evaluated antibiotic resistance, hemolytic, gelatinase, and bile salt hydrolase activities, and the production of biogenic amines and D-lactate using in vitro analyses. We found that <i>L. lactis</i> CKDB001 treatment resulted in significant anti-adipogenic properties in the HepG2 cell line, reducing lipid accumulation and improving lipid profiles through mechanisms involving the upregulation of SIRT1 and PPARα, and downregulation of CD36 and ELOVL6. These results suggest that <i>L. lactis</i> CKDB001 is a safe and effective probiotic for managing MASLD. Further in vivo studies and clinical trials are required to validate these effects and fully elucidate their therapeutic potential and safety profiles.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00949-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142679543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative analysis of seven commonly used synthetic food color additives by HPLC-PDA","authors":"You Rim Min,&nbsp;Jun-Bae Hong,&nbsp;Sam Han,&nbsp;Min-Ji Choi,&nbsp;Seong Bo Shim,&nbsp;Hae-Won Jang,&nbsp;Jung-Bin Lee","doi":"10.1186/s13765-024-00954-y","DOIUrl":"10.1186/s13765-024-00954-y","url":null,"abstract":"<div><p>Sixteen color additives (tar colors) were detected in 128 food samples (macarons, meringue cookies, and coque macarons) using HPLC with a photodiode array detector at 420 nm, 520 nm, and 620 nm for the yellow, red, and blue and green color types, respectively. The tar color recovery rates ranged from 81.3 to 95.6%, and their limits of detection (LOD) and quantification (LOQ) were 0.001–0.049 mg/kg and 0.004–0.147 mg/kg, respectively. Seven tar colors (Y4, Y5, R3, R40, R102, B1, and B2) were detected in 129 samples. All the samples did not contain nine tar colors (R2, G3, Azo, R106, QY, ORII, BBN, PBV, and GS). The quantity of tar colors (Y4, Y5, R40, and B1) in 15 samples exceeded the Korean Ministry of Food and Drug Safety (MFDS) standard. Ninety samples (70%) used a mixture of two or more tar colors, and the amount used was 11.0–1643.3 mg/kg. The quantity of combined tar colors in 15 samples exceeded 300 mg/kg. Through these findings, this study aims to contribute to the development of safer and more reliable desserts containing tar colors, by enhancing safety measures and ensuring improved quality control for consumer protection.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00954-y","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142679699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biosynthesis of phloretin and its C-glycosides through stepwise culture of Escherichia coli","authors":"Shin-Won Lee,&nbsp;Garok Lee,&nbsp;Ji-Hyeon Jo,&nbsp;Youri Yang,&nbsp;Joong-Hoon Ahn","doi":"10.1186/s13765-024-00955-x","DOIUrl":"10.1186/s13765-024-00955-x","url":null,"abstract":"<div><p>Phloretin (PT) belongs to the dihydrochalcones (DHCs) family and is found in apple and rooibos tea. Its glycosides, including phlorizin (PT 2′-<i>O</i>-glucoside), trilobatin (PT 4′-<i>O</i>-glucoside), and nothofagin (NF, PT 3′-<i>C</i>-glucoside), are present in various plants. Phloretin and its related glycosides possess health benefits, including antioxidant, anti-inflammatory, and antibacterial activities. To biosynthesize PT and its glycosides, the relevant pathways in plants were studied and introduced into <i>Escherichia coli</i>. We reconstructed the biosynthetic pathways pertaining to PT and three PT <i>C</i>-glycosides (NF, PT 3′, 5′-di-<i>C</i>-glucoside [PDG], and PT 3′-<i>C</i>-arabinoside [PARA]) in <i>E. coli.</i> To prevent the undesirable synthesis of flavonoids instead of PT, we strategically divided the entire pathway into two parts: the first involved the synthesis of tyrosine to phloretic acid (PA), while the second involved the synthesis of PA to PT and its glycosides. The gene set pertaining to each part was incorporated into a different engineered microbe. We optimized phloretin microbial biosynthesis by improving enzyme affinity, identifying the gene that increased the output, refining the production design to a stepwise culture approach, and analyzing the culture conditions (substrate and yeast extract concentrations and pH) conducive to maximum output and the prevention of product degradation. Using the stepwise culture approach, 12.8 mg/L of PT, 26.1 mg/L of NF, 30.0 mg/L of PDG, and 18.1 mg/L of PARA were synthesized. This study provides valuable information for future approaches in the microbe-based synthesis of PT derivatives.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00955-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142679668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of oral hyaluronic acid on monosodium iodoacetate-induced osteoarthritis in rats: mechanistic insights and therapeutic implications","authors":"Mi-Rae Shin,&nbsp;Minju Kim,&nbsp;Hui Yeon An,&nbsp;Hwang-Yong Choi,&nbsp;Youngseok Ham,&nbsp;Hakjoo Choi,&nbsp;Seong-Soo Roh","doi":"10.1186/s13765-024-00945-z","DOIUrl":"10.1186/s13765-024-00945-z","url":null,"abstract":"<div><p>This study aimed to meticulously assess the effectiveness of hyaluronic acid (HA) in mitigating symptoms associated with monosodium iodoacetate (MIA)-induced osteoarthritis (OA) symptoms in rodent models and to investigate the underlying mechanistic pathways. Eight-week-old rats were randomly allocated to a normal control group and three experimental groups (<i>n</i> = 10 per group). The normal group did not undergo any treatment. The experimental groups were administered MIA for 1 week to induce osteoarthritis, and orally administered distilled water (control group), 2 mg/kg indomethacin (INDO group), or 20 mg/kg HA (HA20 group) daily for 4 weeks. The HA20 group showed a significant improvement in hind-paw weight-bearing distribution after 4 weeks compared to the control group. HA suppressed inflammatory responses by reducing the overproduction of prostaglandin E2 and leukotriene B4 and protected the vital components of the articular ECM, including glycosaminoglycans and aggrecan. HA treatment effectively reduced inflammation, protected cartilage by inhibiting MMP expression, and suppressed inflammatory mediator production. This study demonstrates that HA has potential to alleviate OA symptoms in a rodent model stimulated with MIA, rendering it a promising therapeutic agent for OA.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00945-z","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142645384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeted dsRNA-mediated suppression of Phytophthora infestans infection via Avr3a","authors":"Hyeonmin Lee,&nbsp;Minsu Park,&nbsp;Yujin Kweon,&nbsp;Dowhan Lee,&nbsp;Chanseok Shin","doi":"10.1186/s13765-024-00953-z","DOIUrl":"10.1186/s13765-024-00953-z","url":null,"abstract":"<div><p><i>Phytophthora infestans</i> (<i>P</i>. <i>infestans</i>) is a highly destructive oomycete that causes the late blight in Solanaceous crops, such as potatoes and tomatoes, reducing crop yield. Although many pesticides are used to control <i>P</i>. <i>infestans</i>, the pathogen has evolved resistance to these chemical pesticides over time. In this study, we employed RNAi technology as an alternative strategy to suppress <i>P</i>. <i>infestans</i> infection. We designed and synthesized two dsRNAs targeting 5' and 3' regions of the <i>Avirulence Protein 3a</i> (<i>Avr3a</i>) gene, a key effector essential for the virulence of <i>P</i>. <i>infestans</i>. Interestingly, the dsRNA targeting the 5' region which contains the conserved RxLR-EER motif of Avr3a exhibited more substantial suppression of <i>P</i>. <i>infestans</i> infection and <i>Avr3a</i> expression level compared to the 3' region targeting dsRNA. Additionally, we identified changes in the expression of genes related to pattern-triggered immunity (PTI) and effector-triggered immunity (ETI) in plants treated with these dsRNAs. In leaves treated with dsRNAs targeting <i>Avr3a</i>, the expression of PTI-related genes was restored, while ETI-related genes showed lower expression levels compared to the mock-treated leaves. These results suggest that dsRNAs targeting <i>Avr3a</i> effectively suppress <i>P</i>. <i>infestans</i> infection, enabling plants to achieve balanced immunity and enhanced defense.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00953-z","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142636723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-aging potential of Cephalotaxus harringtonia extracts: the role of harringtonine and homoharringtonine in skin protection","authors":"Si-Young Ahn,&nbsp;Chang-Dae Lee,&nbsp;Ja Jung Ku,&nbsp;Sanghyun Lee,&nbsp;Sullim Lee","doi":"10.1186/s13765-024-00951-1","DOIUrl":"10.1186/s13765-024-00951-1","url":null,"abstract":"<div><p>Photoaging damages the skin layers. The tumor necrosis factor-alpha (TNF-α) plays a crucial role in the central mechanism of photoaging. TNF-α production leads to direct damage to skin cells and facilitates the degradation of vital extracellular matrix (ECM) proteins. TNF-α stimulates matrix metalloproteinase-1 (MMP-1) activation This accelerates the loss of skin elasticity and wrinkle formation. Thus, preventing photoaging and delaying the skin aging process are important research objectives, and the development of new anti-aging substances that target the TNF-α and MMP-1 pathways is promising. In this context, the efficacies of four extracts derived from two types of <i>Cephalotaxus harringtonia</i> (CH) buds (CH-10Y-buds, CH-200Y-buds) and leaves (CH-10Y-leaves, CH-200Y-leaves) were investigated, exhibiting a significant reduction in reactive oxygen species (ROS). Among the four extracts, CH-10Y-buds was the most effective in reducing ROS and exhibited the highest amounts of harringtonine and homoharringtonine. The activities of harringtonine, homoharringtonine, and ginkgetin were evaluated; harringtonine exhibited a high efficacy in inhibiting TNF-α-induced inflammatory responses and MMP-1 activation, thereby reducing collagen degradation. These findings suggest that CH-10Y-buds and their components herringtonin are promising candidates for preventing damage caused by photoaging. Our results can facilitate the development of new methods for maintaining skin health and inhibiting the skin aging process. Further research is necessary to comprehensively evaluate the potential efficacy of these candidate substances and investigate their applicability to actual skin. Such studies will aid in the development of more effective anti-aging strategies in the future.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00951-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142596086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Publisher Correction to: Development of Bacillus stratosphericus Lysate Concentrate to Control Sebum Secretion through In vitro Studies and Clinical Trial","authors":"Hosam Ki,&nbsp;Sung Geon Yoon,&nbsp;Jeung Hi Han,&nbsp;Byeongmin Shin,&nbsp;Young Soo Kim,&nbsp;Yang Gyu Choi,&nbsp;Kwang Yeon Hwang","doi":"10.1186/s13765-024-00950-2","DOIUrl":"10.1186/s13765-024-00950-2","url":null,"abstract":"","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00950-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142565981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro effectiveness of CB469, a MET tyrosine kinase inhibitor in MET-activated cancer cells","authors":"Ji Yeon Song,&nbsp;Hyunsook An,&nbsp;Soojeong Kim","doi":"10.1186/s13765-024-00952-0","DOIUrl":"10.1186/s13765-024-00952-0","url":null,"abstract":"<div><p>Gene alterations in receptor tyrosine kinases can result in oncogenic driver mutation in non-small cell lung cancer (NSCLC) including in genes like EGFR, ALK and MET. MET amplifications and MET exon14 skipping are the primary genetic changes in MET-altered cancers. Acquired MET mutations mediate resistance to clinical MET-targeted therapy in NSCLC. MET kinase domain secondary mutations (D1228X, Y1230X) confer resistance to type I MET tyrosine kinase inhibitors (TKIs) in METexon14-altered or MET amplified NSCLC. Here, we investigated the preclinical activity of a novel MET inhibitor, CB469, with cell growth, signaling pathway and colony formation. We confirmed that CB469 inhibited the activity of MET wild and secondary mutant kinases, D1228N and Y1230H, as a type II inhibitor. CB469 also inhibited cell growth and cell signaling proteins in MET-activated or MET exon14 skipping-mutated cancer cell lines and NIH/3T3 cells expressing an engineered MET mutant. CB469 exhibited the inhibitory efficacy comparable with that of capmatinib in migration of EBC-1(<i>METwt</i>) and Hs746T(<i>METΔex14</i>) cells. Finally, CB469 showed selective and potent inhibition in MET-activated cancer cells among MET TKIs leading to enhanced selectivity for MET-mutant versus wild type MET with inhibition of cell growth in NIH/3T3 cells expressing an engineered MET mutant variant.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00952-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142565825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}