Applied Biological Chemistry最新文献

{"title":"Effect of hydrogen peroxide on the simultaneous removal of veterinary antibiotics during aerobic liquid manure fertilization process","authors":"Young-Kyu Hong,&nbsp;Jin-Wook Kim,&nbsp;Oh-Kyung Kwon,&nbsp;Yong-Bok Lee,&nbsp;Sung Chul Kim","doi":"10.1186/s13765-025-01033-6","DOIUrl":"10.1186/s13765-025-01033-6","url":null,"abstract":"<div><p>Veterinary antibiotics (VAs) in liquid manure-based fertilizers can be applied in the soil and cause an adverse effect on the soil ecosystem. This study evaluates the potential of hydrogen peroxide (H₂O₂) as an oxidant for the simultaneous removal of multiple VA groups during the aerobic swine liquid manure-based fertilization process. For this purpose, swine manure spiked with 10 mg L⁻¹ of target VAs was treated with 1.0% (v v⁻¹) H₂O₂ and then continuously aerated for 28 days in a laboratory-scale aerobic liquid fertilization reactor. The results indicate that 1.0% H₂O₂ effectively degrades penicillins, achieving removal rates exceeding 90% within 24 h. Tetracyclines and macrolides exhibited moderate susceptibility to H₂O₂ oxidation, after 28 days, achieving average removal rates exceeding 90% and 95%, respectively. In contrast, the sulfonamide group displayed the lowest removal efficiency, with an average removal rate of 70%. The study further reveals that the oxidative effect of H₂O₂ is the most pronounced in the early stages of fertilization, with degradation rates plateauing over time due to the gradual decomposition of H₂O₂ and interactions with organic matter in swine manure. Despite these limitations, H₂O₂ treatment did not significantly change the key chemical properties of the final liquid fertilizer, maintaining its fertilizer efficacy. These findings suggest that H₂O₂-based oxidation could be a cost-effective and an eco-friendly substitute for traditional methods for mitigating VA contamination in liquid manure fertilization systems. The results will help to better understand the effect of H₂O₂ on the reduction of VAs during the liquid fertilization process, which has the potential to make a significant contribution to mitigating threats to public health and the ecosystems associated with the application of liquid fertilizer in agricultural environments.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"68 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-025-01033-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145037034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elucidation of the protective effect of schisandrin C originating from Schisandra chinensis (Turcz.) Baill. against indomethacin-induced intestinal injury by combining network pharmacology, molecular docking, and experimental validation","authors":"Son Hung Tran,&nbsp;Uyen Tran Tu Nguyen,&nbsp;Mi Ri Kim,&nbsp;Hee Ju Lee,&nbsp;Esther Youn,&nbsp;Won Young Bae,&nbsp;Suk Woo Kang,&nbsp;Won Kyu Kim,&nbsp;Ki Young Choi,&nbsp;Keunwan Park,&nbsp;Kyungsu Kang","doi":"10.1186/s13765-025-01038-1","DOIUrl":"10.1186/s13765-025-01038-1","url":null,"abstract":"<div><p>Long-term use of nonsteroidal anti-inflammatory drugs (NSAIDs), especially indomethacin, has severe adverse effects on the gastrointestinal system. <i>Schisandra chinensis</i> (Turcz.) Baill., an herbal medicine used to treat several gastrointestinal diseases, might effectively reduce the adverse effects of indomethacin. This study aimed to elucidate the main bioactive compound from <i>S. chinensis</i> and its molecular mechanism for protection against indomethacin-induced intestinal injury. Network pharmacology, molecular docking, and molecular dynamics were performed to elucidate the key compounds of <i>S. chinensis</i>, their relevant targets, and potential molecular mechanisms via various databases, including the TCMSP, SwissTargetPrediction, TargetNet, STRING, and DAVID databases. The protective effect of the key compound and its molecular mechanism were validated in intestinal cells and <i>Caenorhabditis elegans</i> models. Network pharmacology analysis demonstrated that schisandrin C (SCHC) is the active ingredient of <i>S. chinensis</i> and protects against indomethacin-induced intestinal injury. Computational analysis revealed that AKT1, PIK3CA, RELA, EGFR, and GSK3B are potential targets of SCHC. In vitro experiments confirmed that SCHC prevents apoptotic cell death and intestinal permeability dysfunction by modulating the AKT/GSK-3β pathway and tight junction protein expression. Furthermore, SCHC ameliorated the intestinal atrophy induced by indomethacin and increased the intestinal lysosomal level in <i>C. elegans</i>, while its protective effect was abolished in <i>age-1</i> and <i>akt-1</i>,<i>2</i> mutants, indicating the involvement of the PI3K/AKT pathway. In summary, this study demonstrated that SCHC could be a potential candidate for reversing the side effects of indomethacin in the intestinal tract by combining network pharmacology, molecular docking, and experimental validation.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"68 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-025-01038-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145037290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salinity tolerance of two rice cultivars is related to enhanced activities of enzymatic antioxidants and higher proline content","authors":"Tahmina Khanam,&nbsp;Kiyoon Kim,&nbsp;Md. Abdul Karim,&nbsp;Sadia Afrin,&nbsp;Farjana Rahman Lopa,&nbsp;Mohammad Moziball Hoque,&nbsp;Hirokazu Higuchi,&nbsp;Denver I. Walitang,&nbsp;Swapan Kumar Roy,&nbsp;Tongmin Sa,&nbsp;Nahid Akhtar","doi":"10.1186/s13765-025-01034-5","DOIUrl":"10.1186/s13765-025-01034-5","url":null,"abstract":"<div><p>Soil salinity leads to reduced plant health and productivity necessitating the need for salt-tolerant crop cultivars. Thus, the present study evaluated the salinity-induced modulations in morphological, physiological and biochemical responses of two popular rice cultivars bred in Bangladesh, namely BRRI dhan55, which has been developed for growing in dry and pre-monsoon season, and BRRI dhan43, developed for pre-monsoon season. The rice cultivars were exposed to different levels of salt stress (0–300 mM NaCl) after seedling establishment under natural sunshine. Salinity posed a significant growth decline in both the rice cultivars. Under increasing salinity stress, BRRI dhan43 exhibited a pronounced reduction in overall growth. Chlorophyll and proline content declined significantly in BRRI dhan43 with rising salt concentration. A marked decrease in antioxidant enzyme activities, including superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) was also observed. Furthermore, salinity severely reduced grain yield and associated yield parameters in BRRI dhan43. In contrast, BRRI dhan55 demonstrated comparatively better tolerance under salinity. Although chlorophyll content was reduced in BRRI dhan55, the decrease was less pronounced than in BRRI dhan43, while proline content increased with increasing salinity. Additionally, antioxidant enzyme activities (SOD, CAT, and APX) were increased in BRRI dhan55. However, grain yield and yield attributes in BRRI dhan55 were moderately affected by salinity, showing noticeably less reduction compared to BRRI dhan43. Results indicate that the higher salt stress tolerance of BRRI dhan55 is attributed to higher protection of photosynthetic machineries, osmolyte biosynthesis, and upregulated antioxidant functions leading to better yield performance compared to less tolerant rice cultivars.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"68 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-025-01034-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145021589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced diastereomeric mixture analysis through integration of quantum chemical calculations with spatio-conformational information obtained from ultraselective NMR techniques","authors":"Miyeon Bang,&nbsp;Jin Wook Cha","doi":"10.1186/s13765-025-01030-9","DOIUrl":"10.1186/s13765-025-01030-9","url":null,"abstract":"<div><p>Nuclear Magnetic Resonance (NMR) spectroscopy has become indispensable for elucidating molecular structures in pharmaceutical and natural product chemistry, where precise stereochemical configurations critically determine biological activities and therapeutic efficacy. We present an integrated methodology combining ultraselective NMR techniques (GEMSTONE and UHPT) with quantum chemical calculations to analyze iprovalicarb, a conformationally flexible diastereomeric mixture. By extracting detailed individual <i>J</i>-coupling and NOE data from mixture, we established spatio-conformational constraints that enabled systematic filtering of computationally generated conformers. This approach allowed precise identification of conformers consistent with experimental observations and accurate determination of <i>R/S</i> configurations without chemical derivatization or crystallization. ECD calculations on filtered conformers showed superior agreement with experimental measurements compared to unfiltered calculations, validating our approach. This methodology reduces resource requirements while improving structural analysis accuracy, offering applications in drug development and other field of chemistry for complex stereoisomeric systems.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"68 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-025-01030-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145005557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Publisher Correction: Berberine hinders the malignant progression of non-small cell lung cancer via regulating E2F1/FZD8 signaling pathway","authors":"Shenping Liu,&nbsp;Chunmei Tang","doi":"10.1186/s13765-025-01029-2","DOIUrl":"10.1186/s13765-025-01029-2","url":null,"abstract":"","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"68 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-025-01029-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144914776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of sea salt on the fatty liver induced by a high fat diet","authors":"Saoraya Chanmuang,&nbsp;Ye-Jin Son,&nbsp;Quynh-An Nguyen,&nbsp;Dong-Shin Kim,&nbsp;Hyo-Kyoung Choi,&nbsp;Ji-Hye Song,&nbsp;Soo-Hyun Park,&nbsp;Madhuri Sangar,&nbsp;Kyung-Sik Ham,&nbsp;Sang-Mi Jo,&nbsp;Inhae Kang,&nbsp;Hyun-Jin Kim","doi":"10.1186/s13765-025-01028-3","DOIUrl":"10.1186/s13765-025-01028-3","url":null,"abstract":"<div><p>High fat and salt intake link with various health issues, but the combined impact of high fat and salt, especially sea salt (SS) with minerals, on health are still not fully investigated. Therefore, the effect of SS on HFD-induced obese mice was investigated. The study was conducted on male C57BL/6 mice for 13 weeks, following 4 diets: normal diet (control), HFD, HFD with 0.7% SS (LS) and HFD with 2.8% SS (HS). SS countered HFD-induced hepatic fat accumulation and gene expression, accompanied by increased hepatic Ca²⁺ content. SS and Ca²⁺ reduced OA-triggered lipid accumulation in HepG2 cells more effectively than NaCl. Ca²⁺ notably reversed OA-induced reduction in isocitrate dehydrogenase 2 (<i>Idh2</i>) expression in HepG2 cells. However, HFD-altered metabolites were intensified by SS intake. While SS reduced HFD-induced liver fat, not all HFD-impacted liver functions improved. Ca²⁺ in SS may contribute to the regulation of fat accumulation potentially through TCA cycle activation.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"68 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-025-01028-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144880757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seed priming with co-aggregated Azospirillum and Methylobacterium cells reduce the bacterial fruit blotch incidence in watermelon","authors":"Abitha Benson,&nbsp;Kiyoon Kim,&nbsp;Vinodkumar Selvaraj,&nbsp;Rageshwari Selvaraj,&nbsp;Manoharan Melvin Joe,&nbsp;Sa Tongmin","doi":"10.1186/s13765-025-01027-4","DOIUrl":"10.1186/s13765-025-01027-4","url":null,"abstract":"<div><p>Microbial inoculants are increasingly vital in the plant growth-promotion and disease management of important agricultural crops. The possibility of co-aggregated <i>Azospirillum</i> sp. (CW903) and <i>Methylobacterium</i> sp. (CBMB110) as a biocontrol approach to combat watermelon blotch disease was investigated in this study. In survivability assays, co-aggregated CW903 outperformed CBMB110 in the watermelon soil. The co-inoculation using CBMB110 and CW903 in the co-aggregated form drastically reduced the number of <i>Acidovorax citrulli</i> on watermelon leaves and lowered the lesion areas by 42%. Various physiological and biochemical parameters such as phenol concentration, ethylene emission, and trans-cinnamic acid, were significantly decreased by the co-inoculation. Significant decreases were also observed in electrolyte leakage, H₂O₂ concentration, β-1,3-glucanase activity, phenylalanine ammonia-lyase (PAL) activity, peroxidase (POD), and polyphenol oxidase (PPO) activity. Co-aggregated cells enhanced disease suppression efficiency, and significantly reduced the severity of seedling blight by 21% and leaf spot by 35%.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"68 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-025-01027-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144868745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effects of Dioscorea bulbifera and Zingiber officinale mixed extracts against glutamate-induced cell death in HT22 cells","authors":"Seung Woo Im,&nbsp;Won Min Jeong,&nbsp;Dong Yeol Lee,&nbsp;Tae Woo Oh","doi":"10.1186/s13765-025-01026-5","DOIUrl":"10.1186/s13765-025-01026-5","url":null,"abstract":"<div><p>This study aimed to evaluate the neuroprotective effects of mixed extracts from <i>Dioscorea bulbifera</i> and <i>Zingiber officinale</i> (DBZO) against glutamate-induced excitotoxicity in HT-22 cells and to elucidate the associated molecular mechanisms. Neurotoxicity and protective effects were assessed using MTT and LDH assays, while cellular morphology was analyzed via microscopy. DBZO extract significantly restored axonal integrity disrupted by glutamate exposure. A DCFDA assay confirmed that DBZO reduced reactive oxygen species (ROS) generation in a concentration-dependent manner, underscoring its antioxidant capacity. Western blot analysis demonstrated that DBZO markedly decreased glutamate-induced neuronal death at 0.25 and 0.5 mg/mL. The observed neuroprotection was associated with the inhibition of the MAPK signaling cascade and the downregulation of apoptotic markers, including Caspase-3 and PARP. Moreover, DBZO activated the PI3K/Akt/mTOR survival pathway, enhancing neuronal viability. It also boosted antioxidant defenses by modulating Keap1 and NQO1 expression, thereby reducing oxidative damage. Collectively, these findings suggest that DBZO confers neuroprotection by regulating oxidative stress and apoptosis through NRf2/NQO-1 signaling. Due to its strong antioxidant and antiapoptotic properties.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"68 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-025-01026-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144843320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biosynthesis of three benzoic acid derivatives in Escherichia coli","authors":"Hyun-Ah Kim,&nbsp;Hyun Ji Kang,&nbsp;Joong-Hoon Ahn","doi":"10.1186/s13765-025-01024-7","DOIUrl":"10.1186/s13765-025-01024-7","url":null,"abstract":"<div><p>Benzoic acid, an aromatic compound conventionally obtained from coal tar, can also be produced via microbial biotransformation. The shikimate pathway of <i>Escherichia coli</i> provides a route for the biosynthesis of aromatic acids, with its intermediates serving as valuable starting materials for the synthesis of benzoic acid derivatives. Here, we report the <i>E. coli</i>-based synthesis of three benzoic acid derivatives: 3,4-dihydroxybenzoic acid (protocatechuic acid, 3,4-DHBA), gallic acid (GA), and β-glucogallin. The enzyme QuiC was used to catalyze the conversion of 3-dehydroshikimate to 3,4-DHBA. For GA production, a screening of four <i>pobA</i> mutants was conducted to identify the most efficient mutant. A grape-derived uridine diphosphate-dependent glucosyltransferase (UDP-GT) was utilized for the glucosylation of GA to β-glucogallin. To improve the production titers of 3,4-DHBA and GA, a shikimate pathway gene module and a specifically engineered <i>E. coli</i> mutant were employed, resulting in the accumulation of 451.3 mg/L 3,4-DHBA and 123.4 mg/L GA. β-Glucogallin synthesis was achieved through a stepwise process, wherein one <i>E. coli</i> strain produced GA, and its clarified culture medium was subsequently used by a second <i>E. coli</i> strain for β-glucogallin formation. Optimization of the ratio between the GA-containing supernatant and the second cell culture led to a β-glucogallin yield of 118.5 mg/L.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"68 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-025-01024-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144832150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation, flavor compounds, and antioxidant activity of hawthorn fruit-derived maillard reaction products","authors":"Fushen Wang,&nbsp;Shangrong Wang,&nbsp;Cunyong Qin,&nbsp;Jiaxin Li,&nbsp;Zhiping Zhang,&nbsp;Lingbo Ji,&nbsp;Xuewei Jia,&nbsp;Baojiang He,&nbsp;Lei Xing,&nbsp;Chunping Xu,&nbsp;Tianxiao Li","doi":"10.1186/s13765-025-01022-9","DOIUrl":"10.1186/s13765-025-01022-9","url":null,"abstract":"<div><p>In this study, a new kind of hawthorn fruit-derived food flavor was developed through Maillard reaction. The reaction conditions were optimized using a combination of single-factor experiments and response surface methodology (RSM). The key flavor compounds were determined by gas chromatography-mass spectrometry (GC-MS) and relative odor activity values (ROAVs). Single-factor experiments indicated that the preferred conditions were glycine supplement amount of 5.0%, reaction temperature of 130 °C, and reaction time of 90 min, which were further optimized by RSM. GC-MS analysis demonstrated a remarkable 2.7-fold increase in total flavor compounds (TFCs) of Maillard reaction samples (MRSs) than those of enzymolysis extract of hawthorn. ROAV analysis indicated 2,3-dihydro-3,5-dihydroxy-6-methyl-4<i>H</i>-pyran-4-one (DDMP), furfural, 2-methylbutyric acid, hexyl acetate, linalool, decanal, and <i>α</i>-ionone were the key flavor compounds of MRSs, which contributed to the sweetness, roasting, fruity, and acidic aromas. The antioxidant activity were obviously enhanced after Maillard reaction that could be associated with the yielded DDMP and 4-vinylphenol.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"68 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-025-01022-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144814395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}