Applied Biological Chemistry最新文献

{"title":"Ethnomedicine and ethnopharmacology of medicinal plants used in the treatment of diabetes mellitus in Uganda","authors":"Roggers Gang,&nbsp;Motlalepula Matsabisa,&nbsp;Denis Okello,&nbsp;Youngmin Kang","doi":"10.1186/s13765-023-00797-z","DOIUrl":"10.1186/s13765-023-00797-z","url":null,"abstract":"<div><p>Diabetes mellitus (DM) is a global health problem owing to its high prevalence and increased morbidity and mortality. The prevalence of DM and impaired glucose tolerance in Uganda is approximately 4.1% and 6.6%, respectively. Medicinal plants are commonly used for the management of DM, especially in developing countries, such as Uganda<i>.</i> According to several ethnobotanical surveys conducted in Uganda, various medicinal plants are used in DM management. Meanwhile, ethnopharmacological studies have confirmed the anti-diabetic efficacy of various plants and plant-derived formulations from Uganda. However, these information remain highly fragmented without a single repository for plants used in the management and treatment of DM in Uganda, hindering further investigations. Therefore, this study aimed to comprehensively explore plants used for DM treatment in Uganda and retrieve relevant ethnopharmacological and ethnomedicinal information that can be used for DM therapy development. English peer-reviewed articles and books were searched in scientific databases, especially PubMed, Scopus, Google Scholar, Science Direct, SciFinder, and Medline, to retrieve information on medicinal plants used for DM treatment and management in Uganda. The databases were searched to obtain published literature on the anti-diabetic activities and safety of plants among the identified plants. The family name, plant parts used, anti-diabetic activities, dosage, and mechanisms of action of plant extracts were captured. In total, 46 species belonging to 26 families are used to treat DM in Uganda. Most species belonged to the Fabaceae (20%), Asteraceae (13%), and Solanaceae (7%) families. Anti-diabetic activities of 27 (59%) species have been scientifically investigated, whereas the rest have not been evaluated. This review indicated that various medicinal plants are used in the traditional treatment and management of DM across different regions in Uganda. Scientific investigations have revealed the anti-diabetic potential and safety of several of these plants. However, there is a need to validate the anti-diabetic potential of other unstudied plants. Additionally, isolating and characterizing active principles and elucidating the anti-diabetic mechanism of these plants and performing preclinical and clinical studies in the future could aid in the formulation of an effective and safe treatment for DM.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"66 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2023-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-023-00797-z","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4255154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of analysis method for the quantification of residues of halquinol and its metabolites in livestock and fishery products using liquid chromatography–tandem mass spectrometry","authors":"Tae Ho Lee,&nbsp;Sun Young Park,&nbsp;Ji Young Kim,&nbsp;Jang-Duck Choi,&nbsp;Guiim Moon","doi":"10.1186/s13765-023-00793-3","DOIUrl":"10.1186/s13765-023-00793-3","url":null,"abstract":"<div><p>In this study, an analysis method was established for the quantification of residues of halquinol and its metabolites in livestock and fishery products using liquid chromatography–tandem mass spectrometry (LC–MS/MS). We selected beef, pork (muscle and fat), chicken, egg, milk, flat fish, eel, and shrimp as target samples for validation of the method owing to them being typical livestock and fishery products. Validation of the developed analysis method was performed using liquid chromatography–tandem mass spectrometry (LC–MS/MS) at three concentration levels (0.5, 1, and 2 × the maximum residue limits) following the Codex Alimentarius (CODEX) guidelines (CAC/GL 71–2009). For all samples, correlation coefficients (R²) exceeded 0.99, recoveries ranged between 75.59 and 119.36%, and coefficients of variation (CV) ranged between 1.39 and 28.66%, thus satisfying CODEX guidelines. In addition, inter-laboratory validation was conducted, and the resulting recoveries and CVs satisfied the CODEX guidelines; LOQ was established as 10 μg kg^–1 for pig muscle and 5 μg kg^–1 for the other samples. Therefore, the analysis method developed in this study can accurately and precisely screen for and quantify halquinol and its metabolites in livestock and fishery products.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"66 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2023-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-023-00793-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4727276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibition of monoamine oxidases by benzimidazole chalcone derivatives","authors":"Athulya Krishna,&nbsp;Jiseong Lee,&nbsp;Sunil Kumar,&nbsp;Sachithra Thazhathuveedu Sudevan,&nbsp;Prerna Uniyal,&nbsp;Leena K. Pappachen,&nbsp;Hoon Kim,&nbsp;Bijo Mathew","doi":"10.1186/s13765-023-00795-1","DOIUrl":"10.1186/s13765-023-00795-1","url":null,"abstract":"<div><p>Ten benzimidazole chalcone derivatives were synthesized, and their monoamine oxidase (MAO) inhibitory activity was evaluated. Most compounds showed higher inhibitory activity against MAO-B than MAO-A. Compound BCH2 exhibited an IC₅₀ value of 0.80 μM, thereby showing the most potent inhibition amongst all. In addition, BCH2 showed the highest MAO-B selectivity index (SI) with an SI value of 44.11 compared to MAO-A. Among the substituents, the halogen group showed the best MAO-B inhibition, and the <i>ortho</i>-position of the B ring showed better inhibitory activity than the <i>para</i>-site. In comparison with <i>ortho</i>-substituents, the inhibitory activity increased in the order, -Cl &gt; -Br &gt; -F &gt; -H. BCH2 was found to be a competitive inhibitor of the enzyme with optimum inhibition kinetics, where K_i was found to be 0.25 ± 0.014 μM. In the reversibility experiment, BCH2 showed a recovery pattern after MAO-B inhibition, similar to that of lazabemide. Thus, BCH2 is a potent, reversible, and selective MAO-B inhibitor and has been suggested as a candidate for the treatment of neurological disorders.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"66 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2023-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-023-00795-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4687200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The determination of epidermal growth factor in Edible bird's nest by enzyme-linked immunosorbent assay","authors":"Weijuan Bai,&nbsp;Fenghong Deng,&nbsp;Xiaojiang Zhang,&nbsp;Yanping Han,&nbsp;Yue’e Xiao,&nbsp;Nan Wang,&nbsp;Xuncai Liu,&nbsp;Qunyan Fan,&nbsp;Baozhong Guo","doi":"10.1186/s13765-023-00794-2","DOIUrl":"10.1186/s13765-023-00794-2","url":null,"abstract":"<div><p>Edible bird's nest (EBN) is a traditional food which was nourishing and functional. Particularly, there is the epidermal growth factor (EGF) in EBN, which is thought to play an important role in promoting skin repair. However, the type and content of EGF in EBN were not determined yet. In this study, the type of EGF in EBN was identified as bird EGF by enzyme-linked immunosorbent assay and this method was validated to be accurate and precise. Moreover, it was found that the content of EGF in raw-unclean EBN, raw-clean EBN and stewed EBN was 3000 pg/g–4000 pg/g and there were no significant differences, which suggested that the batches, origins, forms, stewing temperatures and stewing times of EBN had no effect on the content of EGF in EBN. However, it was due to that enzyme destroyed the primary structure of EGF, the EGF content of neutral protease and trypsin hydrolysates of EBN was lower than that of flavor enzymes, alkaline protease and pepsin hydrolysates of EGF. This study was the first to determine the type and content of EGF in EBN, and provided a theoretical basis for the selection and processing of EBN and using EBN as a source of EGF.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"66 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2023-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-023-00794-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4388958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of phytochemical profile of rhizome of artificial cultured Polygonatum sibiricum with multiple rhizome buds","authors":"Weiqing Cheng,&nbsp;Zhibin Pan,&nbsp;Hanjing Zheng,&nbsp;Gelian Luo,&nbsp;Zhibin Liu,&nbsp;Suli Xu,&nbsp;Junhan Lin","doi":"10.1186/s13765-023-00792-4","DOIUrl":"10.1186/s13765-023-00792-4","url":null,"abstract":"<div><p>Rhizome of <i>Polygonatum sibiricum</i> is both a renowned traditional Chinese remedy and a commonly consumed delicacy. Due to the escalating demand and excessive overexploitation, there has been a growing interest in the artificial cultivation of this plant in recent years. To assess the therapeutic benefits of artificially cultivated <i>P. sibiricum</i>, it is crucial to identify and classify its phytochemical components, which are the primary bioactive compounds found in its rhizome. In this study, the phytochemical profile of an artificially cultivated <i>P. sibiricum</i> rhizomes with multiple rhizome buds (ACM) was characterized by using untargeted UHPLC-Q-Orbitrap-MS based approach. In addition, two-wild-types <i>P. sibiricum</i> rhizomes, namely the wild-type with multiple rhizome buds (WTM) and the wild-type with single rhizome bud (WTS), were used for comparison. A total of 183 phytochemicals, including 20 alkaloids, 48 flavonoids, 33 phenolic acids, and 82 terpenoids, were tentatively identified. Generally, the phytochemical profile of ACM was comparable to that of WTM and WTS. In specific, most of the identified alkaloids and phenolic acids, and approximately half of the identified terpenoids, were not significantly different. Notably, several phytochemicals with potent therapeutic properties, such as epiberberine, laetanine, sinapic acid, curcumenol, were present in ACM. Additionally, artificial cultivation increased the abundance of geniposide and naringenin, which have been linked to cardioprotective effects. These findings provide valuable insights for the future utilization of artificially cultivated <i>P. sibiricum</i>.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"66 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2023-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-023-00792-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4345185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of antioxidant and anti-inflammatory on bioactive components of carrot (Daucus carota L.) leaves from Jeju Island","authors":"Ji Soo Kim,&nbsp;Ji Hee Lim,&nbsp;Somi Kim Cho","doi":"10.1186/s13765-023-00786-2","DOIUrl":"10.1186/s13765-023-00786-2","url":null,"abstract":"<div><p>The present study evaluated the potential of carrot (<i>Daucus carota</i> L.) leaf, a non-edible part of carrots, which are among the most consumed vegetables worldwide. The antioxidant activities of 70% ethanol extract (EEC) and hot water extract (HEC) of carrot leaves were compared. The results revealed that the total polyphenol content, total flavonoid content, and DPPH and ABTS radical scavenging activities were higher in EEC than in HEC. Both extracts protected the cells against H₂O₂-induced toxicity and markedly reduced the levels of reactive oxygen species in RAW 264.7 cells. Moreover, pretreatment of RAW 264.7 cells with EEC and HEC prior to H₂O₂ (500 μM) exposure increased superoxide dismutase and glutathione peroxidase activities in these cells. Notably, EEC and HEC increased intracellular catalase activity by 36.77 and 6.39 times, respectively. Compared to HEC, EEC remarkably inhibited the production of lipopolysaccharide-induced nitric oxide and reduced the gene expression of IL-6, IL-1β, iNOS, COX-2, and TNF-α. Comparative analysis of the composition of the extracts using HPLC–UV suggested notably higher contents of catechin, chlorogenic acid, caffeic acid, rutin, quercetin, and cynaroside in EEC than in HEC. Collectively, these results imply that carrot leaves are a potentially beneficial natural source of antioxidants and anti-inflammatory compounds in functional foods.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"66 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2023-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-023-00786-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4305037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro repress of breast cancer by bio-product of edible Pleurotus ostreatus loaded with chitosan nanoparticles","authors":"Aisha M. H. Al-Rajhi,&nbsp;Tarek M. Abdel Ghany","doi":"10.1186/s13765-023-00788-0","DOIUrl":"10.1186/s13765-023-00788-0","url":null,"abstract":"<div><p>Despite advances in early detection and therapy, cancer still is a significant health challenge with the highest priority for investigation. Breast cancer represents the most common cancerous disease among women in the world. The study’s purpose is to estimate the cytotoxic activity of the edible mushroom <i>Pleurotus ostreatus</i> extract (PE), chitosan nanoparticles (ChNPs), and PE loaded with ChNPs (PELChNPs), as well as to identify the molecular docking of the cytotoxicity of methyl gallate (MG) as a main component of the PE against breast cancer (MCF-7) cell line. High-performance liquid chromatography (HPLC) analysis of PE exhibited the existence of various phenolic and flavonoid compounds such as MG, gallic acid, chlorogenic acid, hesperetin, naringenin, rutin, and cinnamic acid. The proliferation of the MCF-7 cell line was inhibited at 1, 3.9, and 62.50 µg/mL of PELChNPs, PE, and ChNPs, respectively. PELChNPs were more effective against the MCF-7 cell line than PE, particularly at low concentrations. For instance, at 7.8 µg/mL of PELChNPs and PE, the inhibitory % of MCF-7 proliferation was 20.59±1.75% and 8.57±0.59%, respectively. At 15.6 µg/mL of PELChNPs and PE, the inhibitory % of MCF-7 proliferation was 51.37±1.09% and 25.18±1.64%, respectively. While there is slight difference in the inhibition % of MCF-7 cells (98.64±0.21 and 97.22±0.16%) at high concentration 500 µg/mL of PELChNPs and PE, respectively. IC₅₀ was 15.25 ± 0.54 µg/mL, 46.27 ± 1.94 µg/mL, and 337.38 ± 13.68 µg/mL against MCF-7 cell line of PELChNPs, PE, and ChNPs, respectively. The value of IC₅₀ documented the efficacy of PELChNPs compared with the IC₅₀ (5.91 ± 0.43 µg/mL) of Vinblastine sulfate. Noticeable distortions were observed in the MCF-7 cell line mainly treated with PELChNPs, followed by PE alone. While ChNPs exhibited less effect on the morphology of the MCF-7 cell line. Antioxidant activity of ChNPs, PE, and PELChNPs was evaluated compared with Trolox, which reflected IC₅₀ = 118.33 ± 4.02, 85.63 ± 3.96, 36.80 ± 2.52 and 24.74 ± 0.45 µg/mL. Methyl gallate binding interactions were assessed using molecular docking with the MOE-Dock tool against the target crystal structures of Breast cancer cell line 3HB5. The results shed light on how molecular modeling techniques can inhibit methyl gallate with possible uses in treating breast cancer.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"66 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2023-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-023-00788-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4130151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Induction of autophagy by oleifolioside A in HCT-116 human colorectal cancer cells","authors":"So-Young An,&nbsp;Hyun-Kyu An,&nbsp;Kyoung-Sook Kim,&nbsp;Young-Choon Lee,&nbsp;Seok-Ho Kim","doi":"10.1186/s13765-023-00791-5","DOIUrl":"10.1186/s13765-023-00791-5","url":null,"abstract":"<div><p>In current study, we addressed the anti-cancer effect of oleifolioside A and its mechanism on the regulation of cell death in HCT-116 human colorectal cancer cells. Oleifolioside A inhibited HCT-116 cell proliferation and caused apoptosis associated with sequential activation of caspases 8 and 3, followed by PARP cleavage. Moreover, anti-LC3-positive granules and the increased LC3-II level were observed in HCT-116 cells treated with oleifolioside A, which is the specific characteristics of autophagy. Treatment of autophagy inhibiors, 3-MA and Wort, markedly accelerated the cell death by oleifolioside A and, furthermore, knockdown of Beclin-1 and Atg7 using shRNA increased oleifolioside A-induced apoptosis, suggesting a cytoprotective function of autophagy against oleifolioside A-triggered apoptosis. Treatment of HCT-116 cells with oleifolioside A time-dependently activated extracellular signal-regulated kinase (ERK). Oleifolioside A-induced autophagy was dramatically inhibited by pretreatment with an ERK inhibitor, U0126, which resulted in a marked reduction in cell viability. These findings indicate that oleifolioside A induce autophagy through ERK activation in HCT-116 cells and that autophagy suppression enhances apoptosis induced by oleifolioside A.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"66 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2023-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-023-00791-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4091021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simultaneous quantification of ferrous gluconate and calcium gluconate in foods using liquid chromatography–tandem mass spectrometry (LC–MS/MS)","authors":"Yeong-Ju Park,&nbsp;Sol Sim,&nbsp;Soyeon Jung,&nbsp;Hyunji Seo,&nbsp;Yejin Lee,&nbsp;Jihyun Lee,&nbsp;Chan Lee,&nbsp;Hee-Jae Suh","doi":"10.1186/s13765-023-00790-6","DOIUrl":"10.1186/s13765-023-00790-6","url":null,"abstract":"<div><p>Ferrous gluconate and calcium gluconate are used as food acidity regulators in South Korea, Japan, the European Union (EU), and other countries. A simultaneous analytical method was developed to quantify ferrous gluconate and calcium gluconate in food using ultra-performance liquid chromatography–tandem mass spectrometry. The limits of detection and quantification of ferrous gluconate were 1.1 and 3.5 mg/kg, respectively, while those of calcium gluconate were 1.4 and 4.8 mg/kg, respectively. The recoveries of ferrous gluconate from processed olives were in the range of 97.7–109.7%, while those of calcium gluconate from beverages were in the range of 94.3–110.8%. The developed simultaneous analytical method was applied to real samples from South Korea, which found ferrous gluconate concentrations of 0.031–0.065 g/kg in processed olives and calcium gluconate concentrations of 3.8–7.8 g/kg in beverages.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"66 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2023-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-023-00790-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"5057445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome profiling uncovers the involvement of CmXyn1, a glycosyl hydrolase 11, in Cochliobolus miyabeanus pathogenicity","authors":"Gi Hyun Lee,&nbsp;Ju Soon Yoo,&nbsp;Ha-Ram Oh,&nbsp;Cheol Woo Min,&nbsp;Jeong Woo Jang,&nbsp;Soumya Mukherjee,&nbsp;Ki-Hong Jung,&nbsp;Yu-Jin Kim,&nbsp;Yiming Wang,&nbsp;Ravi Gupta,&nbsp;Sun Tae Kim","doi":"10.1186/s13765-023-00789-z","DOIUrl":"10.1186/s13765-023-00789-z","url":null,"abstract":"<div><p>Necrotrophic pathogen <i>Cochliobolus miyabeanus</i> (<i>C. miyabeanus</i>) causes rice brown leaf spot disease and drastically affects the yield and quality of rice grains. However, the molecular mechanism of rice-<i>C. miyabeanus</i> remains poorly understood due to the limited research conducted on this pathosystem. To elucidate the molecular mechanism of rice-<i>C. miyabeanus</i>, a transcriptome analysis was conducted from in vitro and in planta grown <i>C. miyabeanus.</i> This analysis led to the identification of a total of 24,060 genes of which 426 in vitro and 57 in planta expressed genes were predicted to encode for secretory proteins. As these 57 genes were specifically expressed in planta and were predicted to be secretory in nature, these were consider as putative effectors, highlighting their possible roles in the fungal pathogenicity. Notably, among these putative effectors, <i>CmXyn1</i> which encodes a glycosyl hydrolase 11 displayed the highest expression level under in planta conditions and was thus selected for further functional characterization. Interestingly, the extracellular expression of <i>CmXyn1</i> transiently induced cell death in <i>Nicotiana benthamiana</i> leaves, while intracellular expression was comparatively lesser effective. In addition, transcriptome analysis on rice leaves during <i>C. miyabeanus</i> infection and comparing it to the rice leaf transcriptome data obtained during hemibiotrophic pathogen <i>Magnaporthe oryzae</i> infection led to the discovery of 18 receptors/receptor-like kinases that were commonly expressed in response to both pathogens, indicating their key roles in rice defense response. Taken together, our findings provide new insights into rice-<i>C. miyabeanus</i> interaction as well as the unique and common defense responses of rice against hemibiotroph and necrotroph model systems.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"66 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2023-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-023-00789-z","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4729907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}