{"title":"In Vitro Ruminal Fermentation Profiles of Various Starch-Rich Food By-Products","authors":"Sandi Nayohan, Komura Erina, Masaya Matamura, Yoshimasa Nishikawa, Didier Kichochi Barwani, Hiroki Matsui, Makoto Kondo","doi":"10.1111/asj.70053","DOIUrl":"https://doi.org/10.1111/asj.70053","url":null,"abstract":"<p>This study evaluated nutritional content, gelatinisation rate and rumen fermentation of 13 starch-rich food by-products compared with grain feeds. Using in vitro rumen gas production rates over 24 h, feeds were classified into five clusters. Cluster 1 (corn grits) showed lower rumen fermentability due to low gelatinised starch. Cluster 2 included steam-flaked corn and barley, brown rice tea and barley tea. Cluster 3 (granola, pancake, bread crust, crepe wrapper) exhibited the highest initial gas production rate (<i>p</i> < 0.01), with high sugar and moderate starch. Cluster 4 (ice-cream cone, spring roll wrapper) showed the highest middle-phase gas production rate (<i>p</i> < 0.01), with high gelatinised starch and low sugar. Cluster 5 (ramen, rice cracker, dumpling wrapper, thin wheat noodle) showed slow initial fermentation, accelerating significantly in middle phase. While Clusters 4 and 5 showed the highest organic acid production over 24 h, Cluster 5 had less lactate accumulation in initial and middle phases and a smaller pH decrease compared with Clusters 3 and 4. By-products in Clusters 3, 4 and 5 demonstrated potential to supply more energy to ruminants compared with grain feeds but may increase the risk of ruminal acidosis due to rapid fermentation and subsequent pH reduction.</p>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/asj.70053","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143761851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nu Anh Thu Le, Rena Kubo, Liushiqi Borjigin, Takayuki Ibi, Shinji Sasaki, Tetsuo Kunieda
{"title":"Presence of a Deletion Mutation of Myostatin (MSTN) Gene Associated With Double-Muscling Phenotype in Japanese Black Cattle Population","authors":"Nu Anh Thu Le, Rena Kubo, Liushiqi Borjigin, Takayuki Ibi, Shinji Sasaki, Tetsuo Kunieda","doi":"10.1111/asj.70055","DOIUrl":"https://doi.org/10.1111/asj.70055","url":null,"abstract":"<p>Mutations in the bovine myostatin (<i>MSTN</i>) gene have been identified as the causative factor for the double-muscling phenotype in several European cattle breeds, including Belgian Blue, Piedmontese, and Shorthorn. In Japan, following the Meiji Restoration, several European breeds, including Shorthorn, Brown Swiss, Devon, Simmental, and Ayrshire, were introduced and crossbred with native cattle to develop modern Japanese beef cattle breeds, such as Japanese Black cattle. Historical records regarding the breeding of Japanese Black cattle indicate that the double-muscling phenotype, referred to as “Butajiri,” occasionally appeared in Japanese Black cattle population. These historical observations suggest the potential presence of <i>MSTN</i> gene mutation in the Japanese Black cattle population. The aim of this study was, therefore, to investigate the presence of <i>MSTN</i> gene mutation in the current Japanese Black cattle population. Through screening 400 reproductive females, we identified one cow carrying an 11-bp deletion in the <i>MSTN</i> gene. While further investigation of the animals in the pedigree of this cow could not reveal any living animals with this mutation, this is the first report demonstrating the presence of the <i>MSTN</i> mutation in the Japanese Black cattle population.</p>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/asj.70055","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143749712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yasushi Kiyokawa, Ryoko Koizumi, Ryoko Yamada, Matthew Frye, Michael H. Parsons, Shigeki Hirata, Tsutomu Tanikawa
{"title":"A Nationwide Survey in Japan to Identify the Factors Associated With Rodent Infestation on Livestock Farms","authors":"Yasushi Kiyokawa, Ryoko Koizumi, Ryoko Yamada, Matthew Frye, Michael H. Parsons, Shigeki Hirata, Tsutomu Tanikawa","doi":"10.1111/asj.70057","DOIUrl":"https://doi.org/10.1111/asj.70057","url":null,"abstract":"<div>\u0000 \u0000 <p>Brown rats (<i>Rattus norvegicus</i>), roof rats (<i>Rattus rattus</i>), and house mice (<i>Mus musculus</i>) are major pests on livestock farms. Identifying the factors associated with rodent infestation is considered the first step to control rodents effectively on livestock farms. Here, we performed a nationwide survey in Japan to elucidate the factors associated with rodent infestation. We found that 82.5% of the 401 valid responses indicated that rodent infestation occurred on their farms, suggesting that the presence of rodents is a common occurrence. An ordinal logistic regression analysis suggests that livestock type, farm size, and implementation of rodent control measures contribute to differences in rodent infestation between farms. Comparisons between the most- and least-infested barns on each farm among the 237 valid responses suggest that the number of livestock in the barn on farms keeping cows or pigs, the feeding method on farms keeping pigs, and the age of the barn on farms keeping cows, chickens, or pigs contribute to differences in rodent infestation within a farm. Taken together, the results of this study provide valuable information for understanding rodent infestation on livestock farms.</p>\u0000 </div>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143741373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Dynamic Changes in Postprandial Plasma Free Amino Acid Levels of the Hepatic Portal, Hepatic, and Jugular Veins in the Healthy Pre-Ruminant Calves","authors":"HueyShy Chee, Atsushi Kimura, Aiko Yamamoto-Kinami, Yoshiyuki Tsuchiya, Tomomi Kanazawa, Yuki Hoshino, Keiichi Matsuda, Toshihiro Ichijo","doi":"10.1111/asj.70058","DOIUrl":"https://doi.org/10.1111/asj.70058","url":null,"abstract":"<p>Sufficient amino acids (AAs) supply is crucial in growing animals to maintain the rapid skeletal muscle protein synthesis and healthy growth. Liver is known to be the major organ that plays a central role in AA metabolism. Seeing as few studies have been made to investigate the dynamic changes of postprandial AAs over a short time interval before and after the liver, a first attempt was made to investigate the changes in postprandial free AA levels over eight time points with short interval in plasma, collected simultaneously from the hepatic portal, hepatic, and jugular veins, to better understand the intrahepatic, pre- and post-hepatic AA metabolisms. AAs absorption and uptake by liver occurred soon after feeding and most of the AAs peaked at 30 min postprandial. Two postprandial peaks of the plasma total free AAs, essential AAs (EAAs), and non-essential AAs were observed in hepatic portal and hepatic veins, indicating that two phases of digestion and absorption of AAs may occur in the gastrointestinal tract of the pre-ruminant calves. Individual free AAs showed three main AA transition profiles over time. The total EAA concentration at 240 min postprandial was significantly higher (<i>p</i> < 0.05) in the HPV than in the JV.</p>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/asj.70058","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143740984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effects of Somatic Cell Score on Milk Traits in Polish Holstein-Friesian Cows","authors":"Alicja Satoła","doi":"10.1111/asj.70052","DOIUrl":"https://doi.org/10.1111/asj.70052","url":null,"abstract":"<div>\u0000 \u0000 <p>The objective of this study was to investigate the effect of somatic cell score (SCS) on milk yield; fat, protein, and lactose percentage; and fat-to-protein ratio (FPR). The study dataset contained 41,593 records collected for 2709 Polish Holstein-Friesian cows from three herds. A total of eight classes were created based on SCS. For each study trait, a linear mixed model was used, taking into account fixed effects: parity, stage of lactation, season of calving, SCS class, and their interactions, and random effects of cow, herd test day, and residual. A higher SCS was found to be associated with a reduced milk yield and lactose percentage, while the lowest protein and fat percentage and FPR were observed in the lowest SCS class, and they tended to increase with increasing SCS. The effect of SCS on milk yield, protein, and lactose percentage was more pronounced in younger cows (up to third parity) than in older cows (fifth and sixth parities). A greater effect of SCS on milk yield was observed in the later stages of lactation (from about the fifth month).</p>\u0000 </div>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143749284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nguyen Van Chao, Le Dinh Phung, Ho Thi Dung, Bui Thi Hien, Pham Hoang Son Hung, Tran Quang Vui, Nguyen Thi Hoa, Anjan Mondal, Victor L. Nsereko, Le Duc Thao
{"title":"Effect of Feeding a Saccharomyces cerevisiae Fermentation Product on Pathogenic and Antibiotic Resistance Bacteria in Crossbred F1 (Luong Phuong × Ri) Broiler Chickens in the Production Systems With Low Biosecurity (Sector 3)","authors":"Nguyen Van Chao, Le Dinh Phung, Ho Thi Dung, Bui Thi Hien, Pham Hoang Son Hung, Tran Quang Vui, Nguyen Thi Hoa, Anjan Mondal, Victor L. Nsereko, Le Duc Thao","doi":"10.1111/asj.70049","DOIUrl":"https://doi.org/10.1111/asj.70049","url":null,"abstract":"<div>\u0000 \u0000 <p>This study explored the effects of dietary inclusion of a <i>Saccharomyces cerevisiae</i> fermentation product (SCFP) on bacterial pathogens and associated antimicrobial resistance (AMR) in chickens raised in a Sector 3 production system in Vietnam. A total of 196 one-day-old F<sub>1</sub> (Luong Phuong × Ri) chickens were assigned to two treatments. The treatments were a standard basal diet (control) and a standard basal diet containing 1.25 kg/MT of SCFP (treatment). Fecal samples were collected three times: at 1, 42, and 85 days of age for enumeration of <i>Escherichia coli</i> and isolation of <i>Salmonella</i>. The isolates were tested for AMR and the presence of antimicrobial resistance genes (ARGs) using the disk diffusion and the PCR method. Chickens supplemented with SCFP showed significantly lower of <i>E. coli</i> counts (log CFU/g) compared to the control group at 85 days of age (<i>p</i> = 0.04). There were no differences in the level of AMR of <i>E. coli</i> and <i>Salmonella</i> between the control and treatment groups (<i>p</i> > 0.05). Overall, dietary supplementation of the SCFP did not affect to the frequency of <i>Salmonella</i> colonization and the level of AMR. However, dietary supplementation of the SCFP reduced the <i>E. coli</i> counts at 85 days of age.</p>\u0000 </div>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143707376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effects of Utilizing Spent Coffee Ground as Protein Source for Ruminant Diet by In Vitro Study","authors":"Kanokwan Chukaew, Somkiert Prasanpanich, Phongthorn Kongmun","doi":"10.1111/asj.70048","DOIUrl":"10.1111/asj.70048","url":null,"abstract":"<div>\u0000 \u0000 <p>The aim of this research was to explore the substitution of palm kernel cake (PKC) with spent coffee grounds (SCGs) in ruminant diets through in vitro experiments. The research employed a completely randomized design. These treatments consisted of SCG substituting PKC at 0%, 25%, 50%, 75%, and 100%. Results indicate that SCG contains higher concentrations of CP and EE compared to PKC, while PKC displays elevated levels of NDF and ADF relative to SCG. The cumulative gas production at 72 h exhibited a significant quadratic difference (<i>p</i> < 0.01). IVDMD demonstrated a linear decline with increasing levels of SCG substitution (<i>p</i> = 0.001). The highest ruminal NH<sub>3</sub>-N concentration was observed in the group with 50% SCG substitution, whereas the 75% and 100% substitution groups displayed the lowest values (<i>p</i> < 0.05). At 4 h post incubation, the total VFA concentration and the proportions of C2 and C4 showcased a linear reduction (<i>p</i> < 0.05), whereas the proportion of C3 displayed a significant quadratic difference (<i>p</i> = 0.030), with the 50% substitution level exhibiting the highest. The populations of <i>Ruminococcus albus</i> and <i>Prevotella bryantii</i> after 4 h post incubation experienced a notable linear decrease (<i>p</i> < 0.01). In conclusion, it is recommended to substitute PKC with SCG at levels ranging from 50% to 75%.</p>\u0000 </div>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ismail Cetin, Ece Cetin, Deniz Karakcı, Egemen Ercetin
{"title":"Thyme Essential Oil Supplementation in Growing Merino Lambs: Effects on Growth Performance, Blood Metabolites, and Fecal Microbiology","authors":"Ismail Cetin, Ece Cetin, Deniz Karakcı, Egemen Ercetin","doi":"10.1111/asj.70051","DOIUrl":"10.1111/asj.70051","url":null,"abstract":"<div>\u0000 \u0000 <p>The study was conducted to evaluate the effect of thyme essential oil on growth performance, fecal microflora and immune system parameters in growing Merino lambs to investigate its use as safe and natural method. Thirty Merino lambs were used in 45 days in a randomized complete block design. The lambs in control group did not receive thyme essential oil, whereas each lamb in experimental groups supplemented with 250 mg thyme essential oil/d (T250) and 500 mg thyme essential oil/d (T500) throughout the study. The number of coliform bacteria was found to be the lowest in the T250 group compared to the other groups. Moreover, a significant difference was found between the control and experimental groups in the <i>Lactobacillus</i> (<i>p</i> < 0.019) and <i>Bifidobacterium</i> (<i>p</i> < 0.010) counts, and it was determined that the experimental groups contained higher numbers of beneficial bacteria than the control group. Immunoglobulin G plasma levels was highest in the 500 mg/day thyme oil group compared to the other groups. In conclusion, adding thyme essential oil with a high carvacrol content to lamb rations under stress following weaning has demonstrated that it can have antibacterial, immune-supportive, and growth-promoting properties without causing a negative effect on performance parameters.</p>\u0000 </div>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Weighted Values for Direct and Maternal Genetic Effects to Maximize the Accuracy of Index Selection","authors":"Masahiro Satoh","doi":"10.1111/asj.70050","DOIUrl":"10.1111/asj.70050","url":null,"abstract":"<p>A method was devised to obtain weighted values for the additive direct and maternal genetic effects of a single trait, estimated using a selection index to maximize the accuracy of selection. A method maximizing the square of the selection accuracy was used. This method should facilitate optimal genetic evaluation when the economic weights of direct and maternal genetic effects are unknown or difficult to establish.</p>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11926413/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143668712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Impact of Slaughter Weight on Flavor Metabolites in Longissimus Dorsi Muscle of Tianfu Finishing Pigs","authors":"Xuan Tao, Pinyao Zhao, Yuanfeng Li, Xiang Ao","doi":"10.1111/asj.70045","DOIUrl":"https://doi.org/10.1111/asj.70045","url":null,"abstract":"<div>\u0000 \u0000 <p>In order to investigate the flavor compounds in the muscles of Tianfu finishing (TF) pigs with different slaughter weight (SW), 12 TF pigs were selected for the experiment. The volatile metabolic profiles of meat specimens from the <i>longissimus dorsi</i> (<i>LD</i>) muscle of TF pigs with different SW were studied by untargeted liquid chromatography-mass spectrometry. Our data revealed that the three types of TF pork showed significantly different profiles of hydrocarbons, alcohols, esters, heterocyclic compounds, and others, which could underpin the nuances of their flavors. A total of 118 differentially expressed metabolites (DEMs) were identified (2 upregulated DEMs and 116 downregulated DEMs). Among the volatile flavor compounds, hydrocarbons, alcohols, ester, and heterocyclic compound in the three groups accounted for 26.71%, 13.01%, 13.01%, and 13.70%, respectively, and their contents decreased with increasing SW. The contents of alcohols, aldehydes, and hydrocarbons in the 150-kg group was significantly higher than those in the 125-kg and 100-kg groups (<i>p</i> < 0.05). Taken together, raising SW to 125 kg, or more, decreased the flavor of TF pork and had no benefits to pork quality attributes. Our results provided insights into the molecular basis for sensory variations among different SW of TF pork.</p>\u0000 </div>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143638784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}