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Effect of Feeding Bean Sprout Residues to Cows on Ruminal Gas Production 饲喂豆芽渣对奶牛瘤胃产气的影响
IF 1.7 3区 农林科学
Animal Science Journal Pub Date : 2025-07-23 DOI: 10.1111/asj.70081
Maho Yamanaka, Makito Hori, Toshinori Nakagawa, Masaki Yokoo, Takuji Hirayama
{"title":"Effect of Feeding Bean Sprout Residues to Cows on Ruminal Gas Production","authors":"Maho Yamanaka,&nbsp;Makito Hori,&nbsp;Toshinori Nakagawa,&nbsp;Masaki Yokoo,&nbsp;Takuji Hirayama","doi":"10.1111/asj.70081","DOIUrl":"https://doi.org/10.1111/asj.70081","url":null,"abstract":"<div>\u0000 \u0000 <p>In this study, we investigated the ruminal properties and gas production of cows fed bean sprout residue. Six Holstein cows (average age 24.8 ± 1.0 months) were used and divided into two groups: one that was fed bean sprout residue and one that was not fed bean sprout residue. Three cows were placed in each group in a 2 × 2 crossover design. In the bean sprout residue feeding group, each cow was given 4 kg of raw bean sprout residue in two separate feedings per day, in the morning and in the evening. The survey items were ruminal pH, volatile fatty acid (VFA) concentration, protozoa number, and ruminal gas production, and differences in these items were examined between groups. No significant differences were observed between the groups for any detected VFA concentration. No significant difference was observed in ruminal pH between the groups. The number of protozoa tended to be higher in the bean sprout residue group (<i>p</i> &lt; 0.10). The ruminal methane production was significantly lower in the bean sprout residue-fed group than in the control group (<i>p</i> &lt; 0.01). These results suggest that feeding bean sprout residue may be able to reduce methane emissions without affecting the production of VFAs.</p>\u0000 </div>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144688145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Vitamin B6 Status Assessed by Plasma Vitamers and Homocysteine in Lactating Dairy Cows and Its Relationship to Rumen Fermentation, Plasma Metabolites, and Milk Production in Different Environmental Conditions 不同环境条件下血浆维生素和同型半胱氨酸对泌乳奶牛维生素B6含量的影响及其与瘤胃发酵、血浆代谢产物和产奶量的关系
IF 1.7 3区 农林科学
Animal Science Journal Pub Date : 2025-07-22 DOI: 10.1111/asj.70084
Suttida Prombood, Taketo Obitsu, R-Jun Frederick Avelino Gaspe, Toshihisa Sugino, Yuzou Kurokawa, Thanutchaporn Kumrungsee
{"title":"Vitamin B6 Status Assessed by Plasma Vitamers and Homocysteine in Lactating Dairy Cows and Its Relationship to Rumen Fermentation, Plasma Metabolites, and Milk Production in Different Environmental Conditions","authors":"Suttida Prombood,&nbsp;Taketo Obitsu,&nbsp;R-Jun Frederick Avelino Gaspe,&nbsp;Toshihisa Sugino,&nbsp;Yuzou Kurokawa,&nbsp;Thanutchaporn Kumrungsee","doi":"10.1111/asj.70084","DOIUrl":"https://doi.org/10.1111/asj.70084","url":null,"abstract":"<p>The supply of vitamin B<sub>6</sub> from diet and rumen microbial synthesis is generally considered to be sufficient in dairy cows. However, increased milk yield and environmental factors may alter their vitamin B<sub>6</sub> status. This study aimed to clarify the vitamin B<sub>6</sub> status in lactating dairy cows milked with automatic milking system (AMS) under different environmental conditions. In the winter and summer feeding experiments, plasma concentrations of vitamin B<sub>6</sub> vitamers of pyridoxal (PL) and pyridoxal-5′-phosphate (PLP) with pyridoxic acid (PA), and homocysteine (Hcys) of the cows were assessed, along with their feed intake, milk production, rumen fermentation, and plasma metabolites. Higher PL but lower PA concentrations and PA/(PLP + PL) ratio in plasma were found in the summer experiment compared with the winter experiment, even though plasma PLP concentration, milk production, and dry matter intake were similar between the winter and summer experiments. Plasma concentration of vitamin B<sub>6</sub> vitamers and Hcys concentration did not correlate with milk production parameters. However, the PA/(PLP + PL) ratio in plasma negatively correlated with ruminal acetic acid composition. Summer environment and the variation of rumen fermentation could alter the turnover of vitamin B<sub>6</sub>, even though plasma PLP concentration is likely maintained in lactating cows milked with AMS.</p>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/asj.70084","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144681455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of Bacillus hisashii Supplementation on the Growth Performance, Nitrogen Excretion, and Intestinal Microbiota of Broiler Chickens Fed Low Crude Protein Diet 添加hishii芽孢杆菌对低粗蛋白质饲粮肉鸡生长性能、氮排泄和肠道微生物群的影响
IF 1.7 3区 农林科学
Animal Science Journal Pub Date : 2025-07-22 DOI: 10.1111/asj.70088
Motoi Kikusato, Fu Namai
{"title":"Effect of Bacillus hisashii Supplementation on the Growth Performance, Nitrogen Excretion, and Intestinal Microbiota of Broiler Chickens Fed Low Crude Protein Diet","authors":"Motoi Kikusato,&nbsp;Fu Namai","doi":"10.1111/asj.70088","DOIUrl":"https://doi.org/10.1111/asj.70088","url":null,"abstract":"<div>\u0000 \u0000 <p>The present study evaluated the effects of feeding a newly found thermophile, <i>Bacillus hisashii</i>, on broiler chickens fed a low crude protein (CP) diet. One hundred day-old male Ross 308 broiler chicks are randomly assigned to four treatment groups of five replicates (five birds per replicate). The groups were fed the isocalorically formulated diets containing normal CP (NCP) or low CP (LCP; −2.0% pt) and the diets supplemented with the <i>B. hisashii</i> probiotics (Pro) until 42 days of age. The LCP diet significantly reduced body weight at 42 days, and the probiotics partially suppressed the reduction. There was no difference in feed intake between the groups, and Pro significantly lowered the feed conversion ratio and fecal nitrogen content in the LCP diet (<i>p</i> &lt; 0.05). The probiotics significantly decreased mucosal interleukin-6 concentration in the LCP diet. There were no differences in α-diversity indices of the cecal microbial community, while Pro improved the relative abundance of the genera <i>Lactobacillus</i> and <i>Escherichia-Shigella</i> in the LCP diet. There were significant differences in β-diversity between the groups, excluding NCP/Pro versus LCP. The results suggest that <i>B. hisashii</i> supplementation improved growth performance and intestinal inflammatory status, possibly through improving intestinal microbial composition and balance in broiler chickens.</p>\u0000 </div>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144681315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Partial Substitution of Cereal Grains With Sugar-Rich Castella By-Product on Nutrient Digestibility and Lactation Performance of Dairy Cows 富糖Castella副产品部分替代谷物对奶牛营养物质消化率和泌乳性能的影响
IF 1.7 3区 农林科学
Animal Science Journal Pub Date : 2025-07-22 DOI: 10.1111/asj.70086
Sandi Nayohan, Daiki Kasugai, Masaya Matamura, Yoshimasa Nishikawa, Didier Kichochi Barwani, Makoto Kondo
{"title":"Partial Substitution of Cereal Grains With Sugar-Rich Castella By-Product on Nutrient Digestibility and Lactation Performance of Dairy Cows","authors":"Sandi Nayohan,&nbsp;Daiki Kasugai,&nbsp;Masaya Matamura,&nbsp;Yoshimasa Nishikawa,&nbsp;Didier Kichochi Barwani,&nbsp;Makoto Kondo","doi":"10.1111/asj.70086","DOIUrl":"https://doi.org/10.1111/asj.70086","url":null,"abstract":"<p>This study investigated the effect of partially replacing corn with castella by-product, a sugar-rich feedstuff, on the lactation performance and nutrient digestibility of dairy cows. Eleven late-lactating Holstein cows were used in the experiment employing a crossover design with two periods of 21 days, including 18 days of adaptation and 3 days of data collection, under group-feeding. Castella by-product was incorporated into the diet at 7% on a dry matter (DM) basis, which replaced 30% DM of the starch-rich grains used in the diet. The results showed that the inclusion of the castella increased the digestibilities of DM, organic matter, and neutral detergent fiber (<i>p</i> &lt; 0.05) despite its high sugar content, while decreasing starch digestibility (<i>p</i> &lt; 0.05), without affecting fecal pH, fecal DM, or microbial protein synthesis in the rumen (<i>p</i> &gt; 0.05). The castella diet did not differ significantly from the corn diet for milk yield, milk composition, milk fatty acid profile, or microbial protein synthesis in the rumen (<i>p</i> &gt; 0.05). In summary, the partial replacement of corn with castella by-product in the diet yielded comparable lactation performance outcomes to those of the control diet in dairy cows.</p>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/asj.70086","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144673170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Changes in Antimicrobial Component Concentrations at the Onset of the Dry Period and Peripartum in Dairy Cows 奶牛干燥期和围产期开始时抗菌成分浓度的变化
IF 1.7 3区 农林科学
Animal Science Journal Pub Date : 2025-07-22 DOI: 10.1111/asj.70085
Jirapat Jaisue, Jinkun Sun, Nur Laili Marufah, Masashi Itagaki, Naoki Isobe
{"title":"Changes in Antimicrobial Component Concentrations at the Onset of the Dry Period and Peripartum in Dairy Cows","authors":"Jirapat Jaisue,&nbsp;Jinkun Sun,&nbsp;Nur Laili Marufah,&nbsp;Masashi Itagaki,&nbsp;Naoki Isobe","doi":"10.1111/asj.70085","DOIUrl":"https://doi.org/10.1111/asj.70085","url":null,"abstract":"<p>We aimed to clarify antimicrobial component (AMC) dynamics during the dry period in dairy cows and determine the impacts of varying somatic cell count (SCC) at the onset of the dry period on the subsequent changes in AMC concentrations in milk. Milk samples obtained from Holstein dairy cows were categorized into low-SCC and high-SCC groups. AMC concentrations in milk were measured at four time points: (1) Onset of the dry period, (2) before calving, (3) immediately after calving, and (4) 1 week postpartum. In the low-SCC group, SCC and lingual antimicrobial peptide increased prepartum but decreased 1 week postpartum. Milk lactoperoxidase and lactoferrin concentrations increased prepartum but declined after calving, while cathelicidin-7, S100A7, and S100A8 concentrations increased after calving compared to those prepartum in both groups. S100A7 concentrations in the low-SCC group were significantly higher than those in the high-SCC group prepartum and immediately after calving. However, S100A8 concentrations in the low-SCC group were significantly lower than those in the high-SCC group prepartum and 1 week postpartum. These results suggest that some AMCs were elevated after the onset of the dry period and immediately after calving and highlight the importance of mitigating inflammation in the mammary glands before the onset of the dry period.</p>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/asj.70085","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144673173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Response of the Ruminal Microbial Environment to a High-Dose Supplementation of Blended Essential Oils 大剂量混合精油对瘤胃微生物环境的影响
IF 1.7 3区 农林科学
Animal Science Journal Pub Date : 2025-07-18 DOI: 10.1111/asj.70087
Peng Zhaoyu, Kasumi Fujimura, Alimari Endo, Yutaka Uyeno
{"title":"Response of the Ruminal Microbial Environment to a High-Dose Supplementation of Blended Essential Oils","authors":"Peng Zhaoyu,&nbsp;Kasumi Fujimura,&nbsp;Alimari Endo,&nbsp;Yutaka Uyeno","doi":"10.1111/asj.70087","DOIUrl":"https://doi.org/10.1111/asj.70087","url":null,"abstract":"<div>\u0000 \u0000 <p>We conducted two experiments to investigate the effects of a high-dose blend of essential oils (BEO) on rumen microbial community structure and methane (CH<sub>4</sub>) production. The first was an in vitro study that measured gas production, methane emissions, and microbial composition when two BEO levels (0.1% and 1.0% of the substrate) were added to a forage-based substrate incubated with rumen fluid from dry cows. The second was a feeding trial using a crossover design with four Japanese native goats to evaluate the effects of BEO at 1 g/DM feed on rumen fermentation characteristics and microbial composition. Both experiments demonstrated that BEO supplementation reduced CH<sub>4</sub> production and influenced rumen fermentation parameters. However, BEO altered microbial composition and carbohydrate fermentation patterns differently between the two experiments. In in vitro study, many of the major bacterial groups exhibited population shifts, whereas only one family, Acidoaminococcaceae, decreased in the rumen community of goats fed BEO for 3 weeks. Overall, the results suggest inconsistencies in the microbial response, which may underlie variations in methane emissions and rumen microbiota composition. Our findings imply that BEO broadly suppresses microbial activity, shifting fermentation toward the utilization of readily digestible carbohydrates and leading to reduced methane production.</p>\u0000 </div>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144657707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Detection of QTLs and Candidate Polymorphisms for Percentage of Oleic Acid Through GWAS and Post-GWAS Analysis in Japanese Black Beef 利用GWAS和后GWAS分析检测日本黑牛肉油酸百分比的qtl和候选多态性
IF 1.7 3区 农林科学
Animal Science Journal Pub Date : 2025-07-16 DOI: 10.1111/asj.70083
Fuki Kawaguchi, Natsuki Maeda, Ryotaro Ito, Eiji Iwamoto, Emi Yoshida, Kenji Oyama, Hideyuki Mannen, Shinji Sasazaki
{"title":"Detection of QTLs and Candidate Polymorphisms for Percentage of Oleic Acid Through GWAS and Post-GWAS Analysis in Japanese Black Beef","authors":"Fuki Kawaguchi,&nbsp;Natsuki Maeda,&nbsp;Ryotaro Ito,&nbsp;Eiji Iwamoto,&nbsp;Emi Yoshida,&nbsp;Kenji Oyama,&nbsp;Hideyuki Mannen,&nbsp;Shinji Sasazaki","doi":"10.1111/asj.70083","DOIUrl":"https://doi.org/10.1111/asj.70083","url":null,"abstract":"<div>\u0000 \u0000 <p>Fatty acid composition is a key trait affecting meat quality in Japanese Black cattle. In this study, a genome-wide association study (GWAS) was conducted on Hyogo population of Japanese Black cattle (<i>n</i> = 432) for the percentage of oleic acid (C18:1). Quantitative trait loci (QTLs) were detected on chromosomes 6 and 19 (BTA6 and BTA19). Focusing on the QTL on BTA6, we conducted a detailed search for candidate polymorphisms. Using whole-genome resequencing data (<i>n</i> = 42), all polymorphisms were detected within 83 genes located on the candidate region (chr6: 55–83 Mbp). Based on variant annotations and linkage disequilibrium coefficients with the most significantly associated single nucleotide polymorphism from GWAS (top SNP), 93 variants were extracted as potential causal polymorphisms. Validation revealed that four variants, located within four different genes, had <i>p</i> values comparable to the top SNP. Among them, CWH43 emerged as a strong candidate, as it regulates the intracellular Mn<sup>2+</sup> concentrations, which influence fatty acid metabolism. Notably, the SNP in CWH43 was located upstream of the gene, suggesting a possible regulatory role in gene expression. These findings provide important insights into the genetic basis of fatty acid composition and support the identification of functional polymorphisms in Japanese Black cattle.</p>\u0000 </div>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144647091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Behavior, Welfare, and Meat Quality of Japanese Black Cattle Under Different Transportation Methods 不同运输方式下日本黑牛的行为、福利与肉质
IF 1.7 3区 农林科学
Animal Science Journal Pub Date : 2025-07-16 DOI: 10.1111/asj.70082
Gianne Bianca P. Manalo, Mitsushi Kobayashi, Jitsuo Mizowaki, Masayuki Nagase, Makoto Iwamoto, Kenta Koike, Shigeru Ninomiya
{"title":"Behavior, Welfare, and Meat Quality of Japanese Black Cattle Under Different Transportation Methods","authors":"Gianne Bianca P. Manalo,&nbsp;Mitsushi Kobayashi,&nbsp;Jitsuo Mizowaki,&nbsp;Masayuki Nagase,&nbsp;Makoto Iwamoto,&nbsp;Kenta Koike,&nbsp;Shigeru Ninomiya","doi":"10.1111/asj.70082","DOIUrl":"https://doi.org/10.1111/asj.70082","url":null,"abstract":"<p>Despite extensive research on livestock transportation, the specific effects of different transportation methods on the behavior, welfare, and meat quality parameters of Japanese Black cattle (JBC) remain unclear. This study is the first comprehensive investigation comparing two transport methods: same farm (SF) and multiple farm (MF), while accounting for combined factors such as distance and introduction of unfamiliar animal along the journey. A total of 42 JBC steers (784 ± 81.3 kg) from three production farms (Farms A, B, and C) were observed using focal sampling at a slaughter facility. Generalized linear models (GLMs) were used to analyze the effects of two transportation methods. Results showed that cattle transported via the MF method exhibited significantly higher frequencies of drinking (<i>p</i> = 0.005) and eating (<i>p</i> = 0.005), suggesting increased fatigue. Additionally, self-grooming behavior was significantly higher in Farm B under the SF method (<i>p</i> = 0.002). No significant differences in meat quality parameters were observed between the two transportation methods. These findings suggest that transporting animals from SF method may help reduce transport-related stress upon arrival at the slaughter facility. Further investigation is needed to explore the physiological and economic implications of different transportation methods.</p>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/asj.70082","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144647090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
ceRNA Profiling Reveals circSAMD4A Promoted Porcine Adipocytes Differentiation via Targeting miR-127/PRKAR2B ceRNA分析揭示circSAMD4A通过靶向miR-127/PRKAR2B促进猪脂肪细胞分化
IF 1.7 3区 农林科学
Animal Science Journal Pub Date : 2025-07-01 DOI: 10.1111/asj.70072
Zhenzhen Zhang, Huali Chen, Li Ding, Ya Chen, Chunhui Yin, Pan Zhou, Honglin Yan, Qingbi Gou
{"title":"ceRNA Profiling Reveals circSAMD4A Promoted Porcine Adipocytes Differentiation via Targeting miR-127/PRKAR2B","authors":"Zhenzhen Zhang,&nbsp;Huali Chen,&nbsp;Li Ding,&nbsp;Ya Chen,&nbsp;Chunhui Yin,&nbsp;Pan Zhou,&nbsp;Honglin Yan,&nbsp;Qingbi Gou","doi":"10.1111/asj.70072","DOIUrl":"https://doi.org/10.1111/asj.70072","url":null,"abstract":"<div>\u0000 \u0000 <p>Pork quality is critically influenced by fat deposition patterns, with emerging evidence implicating circular RNAs (circRNAs) as key regulators of adipogenesis, though the specific circRNAs controlling lipogenesis remain incompletely characterized. To systematically elucidate the competing endogenous RNA (ceRNA) network governing porcine adipocyte differentiation, we isolated primary preadipocytes from piglets, induced differentiation, and performed comprehensive RNA sequencing that identified 504 differentially expressed (DE) circRNAs, 126 DE miRNAs, and 1615 DE mRNAs between proliferative and differentiated states, revealing an extensive transcriptomic rewiring during adipogenesis. Functional characterization demonstrated that two circRNAs, circSAMD4A and circLPAR1, significantly promoted lipid accumulation. The further mechanistic studies established that circSAMD4A functions by competitively binding miR-127, alleviating the repression of PRKAR2B, thereby enhancing adipogenic differentiation. These findings not only advance fundamental understanding of circRNA biology in adipose tissue but also provide actionable molecular targets for precision breeding strategies aimed at optimizing intramuscular fat deposition.</p>\u0000 </div>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144520102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparison of Teat End Massage Characteristics Across Liner Types Using a Modeling Device 用建模装置比较不同类型衬垫的乳头末端按摩特性
IF 1.7 3区 农林科学
Animal Science Journal Pub Date : 2025-06-30 DOI: 10.1111/asj.70078
Masafumi Enokidani
{"title":"Comparison of Teat End Massage Characteristics Across Liner Types Using a Modeling Device","authors":"Masafumi Enokidani","doi":"10.1111/asj.70078","DOIUrl":"https://doi.org/10.1111/asj.70078","url":null,"abstract":"<div>\u0000 \u0000 <p>This study evaluated teat end massages (TEMs) associated with various liner shapes and provides foundational data for assessing the impact of liner design on teat ends. A silicon dropper, simulating a teat end, was attached to one end of a stainless tube, with a pressure sensor attached to the opposite end. Twelve liner types were tested under vacuum settings of 40 or 50 kPa, with liners positioned at depths of 7 and 10 cm from the liner mouthpiece. TEMs were recorded over 10-s periods at 0.1-s intervals. TEMs were also conducted after altering the pulsation ratio. TEMs were consistently higher at 50 kPa for all liners and increased at a depth of 10 cm compared with 7 cm. Significant differences (<i>p</i> &lt; 0.01) in TEMs were observed across four categories of liner types, with non-round liners exhibiting higher TEMs. The D-phase duration was correlated with average TEMs, but not with maximum TEMs. Based on these findings, the study recommends using either a round liner with outer ribs or a round silicone liner with a pulsation ratio of 60:40 to promote teat end health.</p>\u0000 </div>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144514700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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