Animal Science Journal最新文献

α-Glycerol Monolaurate Promotes Tight Junction Assembly and Enhances Epithelial Barrier Function in IPEC-J2 Cells and Partridge Chicks α-单月桂酸甘油促进IPEC-J2细胞和鹧鸪鸡紧密连接组装并增强上皮屏障功能

IF 1.7 3区农林科学

Animal Science Journal Pub Date : 2025-06-19 DOI: 10.1111/asj.70067

Siyu Dai, Shuai Meng, Shifeng Liu, Dagang Li, Hong Wang

{"title":"α-Glycerol Monolaurate Promotes Tight Junction Assembly and Enhances Epithelial Barrier Function in IPEC-J2 Cells and Partridge Chicks","authors":"Siyu Dai, Shuai Meng, Shifeng Liu, Dagang Li, Hong Wang","doi":"10.1111/asj.70067","DOIUrl":"https://doi.org/10.1111/asj.70067","url":null,"abstract":"<div>\u0000 \u0000 This study investigated the role and mechanism of α-glycerol monolaurate (α-GML), a Food and Drug Administration–approved natural emulsifier, in enhancing tight junction (TJ) assembly and intestinal barrier function. In vitro experiments showed that α-GML significantly increased the gene and protein levels of TJ-related occludin (OCLN) and zonula occluden-1 (ZO-1), increased ZO-1 distribution in the cell membrane, and reduced paracellular permeability in porcine jejunum epithelial cells (IPEC-J2). These findings indicated that α-GML enhanced intestinal barrier function by promoting TJ protein expression and assembly. Compared with the α-GML group, TJ protein expression and assembly and AMP-activated protein kinase (AMPK) phosphorylation were significantly downregulated in cotreatment groups receiving α-GML along with a calcium-sensing receptor antagonist or a phospholipase C inhibitor. Notably, the groups treated with α-GML and inositol 1,4,5-trisphosphate receptor inhibitor showed a significant decrease in TJ assembly and AMPK phosphorylation, with no significant difference in the TJ protein expression levels. In vivo experiments demonstrated that dietary α-GML significantly upregulated TJ levels and AMPK phosphorylation in the jejunum of partridge chicks. In conclusion, α-GML may upregulate intestinal epithelial TJ assembly via AMPK activation and promote intestinal barrier development in young animals to maintain intestinal health.\u0000 </div>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144323504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Individual Variation in Salt Block Licking and Its Relationship With the Social Hierarchy of Resource Access in Beef Cows: A Case Study 肉牛舔盐块的个体差异及其与资源获取社会等级的关系

IF 1.7 3区农林科学

Animal Science Journal Pub Date : 2025-06-10 DOI: 10.1111/asj.70070

Hirohiko Kume, Kaoru Sato, Michiru Fukasawa, Shin-ichiro Ogura

{"title":"Individual Variation in Salt Block Licking and Its Relationship With the Social Hierarchy of Resource Access in Beef Cows: A Case Study","authors":"Hirohiko Kume, Kaoru Sato, Michiru Fukasawa, Shin-ichiro Ogura","doi":"10.1111/asj.70070","DOIUrl":"https://doi.org/10.1111/asj.70070","url":null,"abstract":"This study aimed to evaluate the relationship between the licking time of salt block (LT) and the social hierarchy (SH) of resource access of cows. LT and SH were determined for 20 days using 19 Japanese Black beef cows under group-feeding, and then classified into three rank groups (low, medium, and high) based on their SH ranking. The average LT showed significant variations among individuals (p < 0.05), ranging from 17.9 to 1124.3 s/day. In the GLM analysis, there were no significant effects of SH, parity, or body weight on LT (p > 0.10). Although there was no correlation between the LT and the SH (p > 0.1, ρ = −0.08), and LT was not consistently associated with the social hierarchy, the low SH group tended to have greater individual variation in LT than high SH group (p = 0.053). Lower SH cows may experience either enhanced or reduced motivation for salt block licking due to behavioral interruption by higher SH cows, which could reduce their opportunities to display a medium LT.","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/asj.70070","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144256022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of Dietary Nucleotide Supplementation in Milk Replacer on Growth Performance and Blood Composition of Japanese Black Suckling Calves 饲粮中添加代乳剂核苷酸对日本黑乳犊牛生长性能和血液成分的影响

IF 1.7 3区农林科学

Animal Science Journal Pub Date : 2025-06-09 DOI: 10.1111/asj.70064

Masahiro Shibata, Sara Yasuhara, Jiro Sasaki, Masahiko Tabata, Masahiro Osada

{"title":"Influence of Dietary Nucleotide Supplementation in Milk Replacer on Growth Performance and Blood Composition of Japanese Black Suckling Calves","authors":"Masahiro Shibata, Sara Yasuhara, Jiro Sasaki, Masahiko Tabata, Masahiro Osada","doi":"10.1111/asj.70064","DOIUrl":"https://doi.org/10.1111/asj.70064","url":null,"abstract":"<div>\u0000 \u0000 The present study aimed to investigate the effects of dietary nucleotide supplementation of an antibiotic-free milk replacer on the growth performance and blood composition of Japanese Black calves. Eighteen calves were divided into two groups: antibiotics-free milk replacer supplementation with (RM group) and without (CT group) nucleotides. No differences in dry matter, crude protein, or total digestible nutrient intake were found between the groups during the suckling period. The suckling period from the beginning of the study at age 11.6 ± 0.9 days to weaning was significantly shorter in the RM group than in the CT group. Body weight was not significantly different between the two groups at any time point. However, the daily gain (DG) and feed efficiency during the suckling period were significantly higher in the RM group than in the CT group. Body measurements and fecal condition of calves were recorded, but no significant differences in any values were observed between the two groups. Post-weaning blood β-hydroxybutyrate values in both groups were significantly higher than those in the suckling period. Feeding nucleotides to Japanese Black suckling calves led to enhanced growth performance, increased DG, and improved feed efficiency, resulting in a shorter duration of suckling.\u0000 </div>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144244958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Crimson Clover Silage Inclusion on Nutrient Intake, Behavior, and Stress Condition in Gestating Sows 苜蓿青贮包埋对妊娠母猪营养摄入、行为和应激状态的影响

IF 1.7 3区农林科学

Animal Science Journal Pub Date : 2025-06-08 DOI: 10.1111/asj.70065

Andrew L. Makalani, Yasuyuki Ishii, Tomoyuki Kawashima, Ikuo Kobayashi, Toshihiro Takahashi

{"title":"Effects of Crimson Clover Silage Inclusion on Nutrient Intake, Behavior, and Stress Condition in Gestating Sows","authors":"Andrew L. Makalani, Yasuyuki Ishii, Tomoyuki Kawashima, Ikuo Kobayashi, Toshihiro Takahashi","doi":"10.1111/asj.70065","DOIUrl":"https://doi.org/10.1111/asj.70065","url":null,"abstract":"<div>\u0000 \u0000 This study aimed to determine whether crimson clover silage (CSS) can serve as an effective and beneficial substitute for conventional sow diets, to enhance satiety and normal behavior, while reducing production costs. Six sows with an average initial body weight (BW) of 246.0 ± 16.0 kg and an initial backfat thickness of 23.8 ± 1.10 mm were assigned to a 6 × 2 crossover design. Each feeding period included a 10-day acclimation period and a 10-day data collection period for each treatment. In the test treatment, a commercial diet was replaced by CCS on a dry matter (DM) basis at 20%, while the control treatment had no CCS inclusion. Data on daily feed intake, BW, and backfat thickness were recorded, and urine samples for cortisol analysis and video footage for behavior analysis were collected on the last day of each treatment. There were no leftovers with either diet, and nutrient uptake, BW, and backfat thickness were unaffected (p > 0.05). However, stereotypic behaviors and urinary cortisol concentrations were significantly reduced (p < 0.05) with CCS inclusion. Replacing 20% DM of the commercial diet with crimson clover silage presents an effective strategy for lowering feeding costs while also enhancing animal behavior and well-being.\u0000 </div>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144244530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced Retinol Extraction From Bovine Whole Blood Using n-Hexane and Phosphate-Buffered Saline 正己烷和磷酸盐缓冲盐水强化牛全血视黄醇提取

IF 1.7 3区农林科学

Animal Science Journal Pub Date : 2025-06-05 DOI: 10.1111/asj.70069

Wataru Iwasaki, Nobutomo Morita, Risa Yokoishi, Tetsuro Inoue, Takanori Jogi, Seiyu Miki

{"title":"Enhanced Retinol Extraction From Bovine Whole Blood Using n-Hexane and Phosphate-Buffered Saline","authors":"Wataru Iwasaki, Nobutomo Morita, Risa Yokoishi, Tetsuro Inoue, Takanori Jogi, Seiyu Miki","doi":"10.1111/asj.70069","DOIUrl":"https://doi.org/10.1111/asj.70069","url":null,"abstract":"Ensuring optimal blood retinol levels of cattle during fattening is essential for achieving high-quality marbling in beef production. We previously developed a rapid analytical method for measuring blood retinol concentration that eliminates the process of blood cell separation. However, achieving a strong correlation with actual retinol levels proved challenging due to the requirements of vigorous agitation of blood and n-hexane using a vortex mixer. Consequently, in this study, we developed a modified method that does not require intense agitation by adding phosphate-buffered saline (PBS) to the blood and n-hexane mixture. The addition of PBS facilitated the dispersion of whole blood in n-hexane, although the augmented interface between the two enhanced extraction efficiency. Furthermore, we conducted field tests using blood samples collected from 98 fattening cattle from six different farms, each using distinct feeding methods and rearing environments, to reflect the diverse feeding practices in Japanese Black beef production. Their retinol concentrations were analyzed using the developed method and compared with values obtained by HPLC. A correlation coefficient of 0.94–0.96 was obtained, indicating that blood retinol levels can be reliably measured even among farms with different feeding methods.","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/asj.70069","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144220128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dietary Supplementation With Mannitol Regulates the Rumen and Intestinal Microbiome, Immune Function, and Antioxidant Capacity of Grazed Mongolian Sheep 饲粮中添加甘露醇对放牧蒙古羊瘤胃和肠道微生物群、免疫功能和抗氧化能力的调节

IF 1.7 3区农林科学

Animal Science Journal Pub Date : 2025-06-03 DOI: 10.1111/asj.70074

Xiao Min, Aldar, Gong Jian

{"title":"Dietary Supplementation With Mannitol Regulates the Rumen and Intestinal Microbiome, Immune Function, and Antioxidant Capacity of Grazed Mongolian Sheep","authors":"Xiao Min,  Aldar, Gong Jian","doi":"10.1111/asj.70074","DOIUrl":"https://doi.org/10.1111/asj.70074","url":null,"abstract":"<div>\u0000 \u0000 This study aimed to investigate the short-term effects of mannitol supplementation on grazing Mongolian sheep, including ruminal and intestinal microbiome, serum biochemical parameters, and immune and antioxidant functions. Ten sheep were randomly divided into control and experimental groups (n = 5) receiving without or with 10 g/kg mannitol, respectively. After 20 days, including a 5-day adaptation, mannitol did not affect the average daily gain and rumen fermentation but significantly altered microbial composition, as evidenced by principal coordinates' analysis. The abundances of genera including Rikenellaceae_RC9_gut_group increased notably in the rumen, and those of Alistipes and Norank_f_Muribaculaceae increased in intestine with mannitol supplementation. Mannitol increased the serum concentrations of total protein, albumin, retinol-binding protein, paraoxonase, and total cholesterol and decreased alanine aminotransferase levels. Mannitol also increased the levels of immunoglobulins, interleukins, tumor necrosis factor, interferon, and complements and antioxidant enzyme activities while reducing malondialdehyde and hydrogen peroxide concentrations. The serum total protein positively correlated with Rikenellaceae_RC9_gut_group and Alistipes while negatively correlated with superoxide dismutase and specific microbial genera. Taken together, mannitol supplementation reshapes ruminal and intestinal microbial communities, improving serum biomarkers, immune responses, and antioxidant defenses in grazing Mongolian sheep. These findings offer insights into novel feeding strategies for the management of grazing sheep.\u0000 </div>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144206386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Various Factors, Including Management Type at the Slaughterhouse, on the Presence of Dark Cutting in Cattle: A Study in Turkey 包括屠宰场管理类型在内的各种因素对牛黑切现象的影响：土耳其的一项研究

IF 1.7 3区农林科学

Animal Science Journal Pub Date : 2025-06-03 DOI: 10.1111/asj.70073

Seyfi Özdemir, Bulent Ekiz

{"title":"Effects of Various Factors, Including Management Type at the Slaughterhouse, on the Presence of Dark Cutting in Cattle: A Study in Turkey","authors":"Seyfi Özdemir, Bulent Ekiz","doi":"10.1111/asj.70073","DOIUrl":"https://doi.org/10.1111/asj.70073","url":null,"abstract":"The aim of this study was to examine the effects of various factors, especially the management type applied to cattle in the slaughterhouse, on meat pH24h and the presence of dark cutting. Data from 583 cattle of the Holstein and Simmental breeds were evaluated. Cattle were divided into four management types according to whether they were tethered or nontethered in the unloading, lairage, and slaughter corridor. Mean pH24h was 5.60, and the dark-cutting percentage was 13.20%. The effect of management type on the presence of dark cutting was significant (p = 0.047). The mean pH24h value was higher in cattle from the nontethered management group (G-I, 5.642) than in cattle from the tethered group (G-II, 5.579). The presence of dark cutting was 2.519 times higher in the G-I cattle than in the cattle in Group IV (tethered during unloading and at the lairage and nontethered at the slaughter corridor). The effects of season, sex, season × sex, season × management type, sex × management type, age, trackload density, and carcass weight on pH24h were significant. In conclusion, the tethered management type may contribute to reducing the presence of dark cutting in slaughterhouses that were established without considering animal behaviors and ease of cattle handling.","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/asj.70073","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144206385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of Somatic Cell Score at First Calving With Milk Productivity and Mortality in Dairy Herds: A Cross-Sectional Study in Eastern Hokkaido, Japan 日本北海道东部奶牛群初产犊时体细胞评分与产奶量和死亡率的相关性研究

IF 1.7 3区农林科学

Animal Science Journal Pub Date : 2025-06-02 DOI: 10.1111/asj.70071

Akira Goto, Mitsuo Yokoi, Naoki Yamamoto, Yoichi Inoue, Keiichi Hisaeda, Yasunori Shinozuka, Ken Nakada

{"title":"Association of Somatic Cell Score at First Calving With Milk Productivity and Mortality in Dairy Herds: A Cross-Sectional Study in Eastern Hokkaido, Japan","authors":"Akira Goto, Mitsuo Yokoi, Naoki Yamamoto, Yoichi Inoue, Keiichi Hisaeda, Yasunori Shinozuka, Ken Nakada","doi":"10.1111/asj.70071","DOIUrl":"https://doi.org/10.1111/asj.70071","url":null,"abstract":"<div>\u0000 \u0000 Over the past decade, the increasing proportion of primiparous cows in Hokkaido dairy herds has resulted in younger herds, but somatic cell count (SCC) has stagnated at around 220,000 cells/mL, preventing milk quality improvements. This study evaluated the impact of elevated postpartum somatic cell scores (SCS) in primiparous cows on herd productivity, aiming to inform milk quality improvement measures in Hokkaido. Production data from 163 herds in Eastern Hokkaido were analyzed, with milk yield per cow, herd SCC, and mortality used as dependent variables and primiparous cow SCS as the independent variable. Results revealed a significant positive association between primiparous cow SCS and both herd SCC and mortality, whereas a significant negative association was observed with milk yield per cow. These findings suggest that high SCS in primiparous cows may reflect suboptimal animal welfare practices, such as poor udder hygiene and inadequate peripartum management. Enhancing welfare practices, such as udder hygiene, and providing a supportive calving environment could improve SCS in primiparous cows, increase milk productivity, and reduce mortality. Additionally, supporting farmers by using SCC levels of primiparous cows as a benchmark could be an effective strategy for improving milk quality in the Hokkaido region.\u0000 </div>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144197459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Equol-Producing Bacteria in the Chicken Intestine: PCR Analysis on the Bacteria Quantitatively Correlating With Equol Concentration in Daidzein-Fed Laying Hens 鸡肠内产马酚细菌：用大豆素饲养的蛋鸡体内马酚浓度与细菌数量相关的PCR分析

IF 1.7 3区农林科学

Animal Science Journal Pub Date : 2025-05-31 DOI: 10.1111/asj.70068

Sota Sakai, Kyohei Furukawa, Fu Namai, Keita Nishiyama, Dipson Gyawali, Tatsuhiko Goto, Motoi Kikusato

{"title":"Equol-Producing Bacteria in the Chicken Intestine: PCR Analysis on the Bacteria Quantitatively Correlating With Equol Concentration in Daidzein-Fed Laying Hens","authors":"Sota Sakai, Kyohei Furukawa, Fu Namai, Keita Nishiyama, Dipson Gyawali, Tatsuhiko Goto, Motoi Kikusato","doi":"10.1111/asj.70068","DOIUrl":"https://doi.org/10.1111/asj.70068","url":null,"abstract":"<div>\u0000 \u0000 Equol is a microbial metabolite from daidzein, an isoflavone, in the animal intestine. This study aimed to identify equol-producing bacteria in the intestines of chickens. Sixteen 181-day-old Hy-Line Brown laying hens were given a basal diet formulated excluding soy products for 2 weeks and subsequently given the diets supplemented with 0-, 100-, 1000-, and 10,000-ppm daidzein for 10 days. Plasma and egg yolk equol concentration was significantly increased with dietary daidzein supplementation. Gene abundance of two major bacterial enzymes catalyzing the conversion of daidzein to equol, dihydrodaidzein reductase, and tetrahydro daidzein reductase was measured using polymerase chain reaction (PCR) analysis, with the abundance increasing with dietary daidzein supplementation (p < 0.05). After sequencing the PCR amplicons, six possible bacteria were identified, and they were further identified and quantified using a specific primer for each bacterial gene. As a result, Adlercreutzia equolifaciens and Slackia equolifaciens were identified, and their bacterial relative abundance increased with dietary daidzein concentration and plasma equol levels (p < 0.05). A. equolifaciens and S. equolifaciens are known as equol-producing bacteria, and the present study was the first to show that these bacteria could also contribute to equol production in chickens.\u0000 </div>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144179250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improvement of Sperm Quality in Cryopreserved Boar Semen by Supplementation of Kaempferia parviflora and Cholesterol-Loaded Cyclodextrin in Semen Extender 增精剂中添加山柰和高胆固醇环糊精对猪冷冻精液精子质量的改善

IF 1.7 3区农林科学

Animal Science Journal Pub Date : 2025-05-28 DOI: 10.1111/asj.70066

Himalai Saiyamanon, Ruthaiporn Ratchamak, Khomsan Buathalad, Wuttigrai Boonkum, Vibuntita Chankitisakul

{"title":"Improvement of Sperm Quality in Cryopreserved Boar Semen by Supplementation of Kaempferia parviflora and Cholesterol-Loaded Cyclodextrin in Semen Extender","authors":"Himalai Saiyamanon, Ruthaiporn Ratchamak, Khomsan Buathalad, Wuttigrai Boonkum, Vibuntita Chankitisakul","doi":"10.1111/asj.70066","DOIUrl":"https://doi.org/10.1111/asj.70066","url":null,"abstract":"<div>\u0000 \u0000 We investigated the potential of Kaempferia parviflora (KP) extract, rich in phenolic compounds, for enhancing boar semen quality during cryopreservation. We evaluated the impact of various KP extract concentrations (0%–8% w/v) in freezing extenders on postthawed semen quality and oxidative stress markers, along with the effects of cholesterol-loaded cyclodextrins (CLC). Semen was frozen using liquid nitrogen vapor before being plunged into liquid nitrogen, and postthaw semen quality and lipid peroxidation were analyzed. Results showed that 2%–4% KP extract significantly improved total and progressive motility, sperm viability, and acrosome integrity compared to the control (p < 0.05), likely due to reduced lipid peroxidation indicated by lower malondialdehyde (MDA) levels (p < 0.05). However, higher concentrations (6%–8%) negatively affected sperm quality. Adding CLC further enhanced the positive effects of KP extract, improving sperm motility and acrosome integrity and reducing lipid peroxidation (p < 0.05). In conclusion, supplementation of boar semen cryopreservation media with 2% KP extract significantly enhanced sperm quality postthawing, likely through its potent antioxidant properties. Combining KP extract with CLC amplified these beneficial effects. These findings highlight a promising natural strategy for improving boar semen cryopreservation outcomes.\u0000 </div>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144171206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0