Animal Science Journal最新文献

Responses of Chick Myoblast Proliferation to Branched-Chain Amino Acids in a Three-Component System 三组分体系中支链氨基酸对鸡成肌细胞增殖的影响。

IF 1.2 3区农林科学

Animal Science Journal Pub Date : 2025-09-19 DOI: 10.1111/asj.70106

Kunihiko Soma, Motoi Kikusato, Kan Sato, Masaaki Toyomizu

{"title":"Responses of Chick Myoblast Proliferation to Branched-Chain Amino Acids in a Three-Component System","authors":"Kunihiko Soma, Motoi Kikusato, Kan Sato, Masaaki Toyomizu","doi":"10.1111/asj.70106","DOIUrl":"10.1111/asj.70106","url":null,"abstract":"<div>\u0000 \u0000 This study investigated the combined effect of branched-chain amino acids (BCAAs), valine (Val), leucine (Leu), and isoleucine (Ile) on chick myoblast proliferation and associated gene expression within a three-component system. Isolated myoblasts were incubated in 22 different culture media, designed using a {3, 5} simplex lattice design to cover the entire range of compositions for each BCAA. The proliferation was estimated based on cell quantities after 72 h of incubation. The proliferation data under different BCAA combinations were fitted using Scheffé's seven-term special cubic model for three components. The response surfaces of proliferation showed a convex configuration, with increases in Ile leading to a decrease in proliferation. The maximal proliferation was observed at Leu and Val proportions of 3:7 and 1:1 in an Ile-free medium, as measured by the DNA assay and water-soluble tetrazolium salt (WST-8) assay, respectively. These findings suggest that Val has a greater influence than Leu on myoblast proliferation, particularly in DNA content. The responses of myoblast proliferation-associated factors, Pax7 and p21, corresponded to the proliferation evaluated with DNA and WST-8 assays, respectively. These results indicate that the growth of chick myoblast cells is affected by the combination of BCAAs in a three-component system.\u0000 </div>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145084948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chitosan Oligosaccharide Reduces Abdominal Fat Deposition by Regulating Cecal Microbiota Composition in Broilers 壳聚糖低聚糖通过调节盲肠菌群组成减少肉鸡腹部脂肪沉积

IF 1.2 3区农林科学

Animal Science Journal Pub Date : 2025-09-12 DOI: 10.1111/asj.70105

Ruixia Lan, Haoxuan Wang, Jia Zhang, Adzitey Frederick

{"title":"Chitosan Oligosaccharide Reduces Abdominal Fat Deposition by Regulating Cecal Microbiota Composition in Broilers","authors":"Ruixia Lan, Haoxuan Wang, Jia Zhang, Adzitey Frederick","doi":"10.1111/asj.70105","DOIUrl":"https://doi.org/10.1111/asj.70105","url":null,"abstract":"<div>\u0000 \u0000 To illustrate the effects of chitosan oligosaccharide (COS) supplementation on abdominal fat deposition (AFD), lipid metabolism, cecal microbiota composition, and short-chain fatty acids (SCFA) content in the ceca of broilers. Totally, 144 one-day-old male Arbor Acres broilers were randomly allocated into two groups with six replicates and 12 broilers per replicate. The control group (CON) was fed the basal diet; the treatment group was fed the basal diet with 200-mg/kg COS (COS200). COS supplementation led to a reduction (p < 0.05) in AFD, serum triglyceride, hepatic high-density lipoprotein cholesterol content, hepatic fatty acid synthase, acetyl-coenzyme A carboxylase gene expression level, and peroxisome proliferator-activated receptor γ gene expression level in abdominal fat (AF). Furthermore, COS supplementation resulted in higher (p < 0.05) relative abundance of cecal Bacteroidetes and Alistipes but lower (p < 0.05) relative abundance of Desulfobacterota, Patescibacteria, Campilobacterota, Deferribacterota, Shuttleworthia, and Erysipelatoclostridium, accompanied by increasing acetic acid, propionic acid, isobutyric acid, caproic acid, and total acid content. The AF weight inversely (p < 0.05) correlated with the relative abundance of Bacteroidota and isobutyric acid content. Overall, COS supplementation reduced AF deposition by inhibiting hepatic fatty acid synthesis, abdominal adipocyte differentiation, and proliferation-related gene expression, which was associated with the changes in cecal microbiota composition and SCFA content.\u0000 </div>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145038432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Re-Vitrification at the Pronuclear and Blastocyst Stage of Porcine Embryos Obtained From Vitrified Immature Oocytes 猪未成熟卵母细胞在原核和囊胚期的再玻璃化。

IF 1.2 3区农林科学

Animal Science Journal Pub Date : 2025-09-11 DOI: 10.1111/asj.70103

Hiep Thi Nguyen, Michiko Nakai, Shoichiro Sembon, Nguyen Xuan Bui, Uoc Thi Nguyen, Kazuhiro Kikuchi, Seiki Haraguchi, Linh Viet Nguyen, Hong Thi Nguyen, Nhung Thi Nguyen, Hanh Van Nguyen, Duc Tien Luu, Tamás Somfai

{"title":"Re-Vitrification at the Pronuclear and Blastocyst Stage of Porcine Embryos Obtained From Vitrified Immature Oocytes","authors":"Hiep Thi Nguyen, Michiko Nakai, Shoichiro Sembon, Nguyen Xuan Bui, Uoc Thi Nguyen, Kazuhiro Kikuchi, Seiki Haraguchi, Linh Viet Nguyen, Hong Thi Nguyen, Nhung Thi Nguyen, Hanh Van Nguyen, Duc Tien Luu, Tamás Somfai","doi":"10.1111/asj.70103","DOIUrl":"10.1111/asj.70103","url":null,"abstract":"<div>\u0000 \u0000 The aims of this study were to investigate the effects of re-vitrification at the pronuclear (PN) stage of porcine embryos generated from vitrified oocytes on subsequent development and to clarify if re-vitrification is more feasible at the PN stage or at the blastocyst stage. Immature porcine oocytes at the germinal vesicle (GV) stage were vitrified/warmed and subjected to in vitro maturation, parthenogenetic activation (PA), and embryo culture. Subsequent parthenotes were either cultured without re-vitrification for 6 days (GV-vit group) or were re-vitrified 8 h after PA at the PN stage (GV-vit/PN-revit group), and after warming, cultured for 6 days. Embryo development to the blastocyst stage was compared with a control group processed without vitrification at any stage. Then, blastocysts obtained in each group were vitrified/warmed and their survival was assessed. Blastocyst formation in the GV-vit/PN-revit group decreased dramatically (p < 0.05) compared with the GV-vit and control groups (3.4% vs. 10.2% and 22.4%, respectively). However, 80.0% of the blastocysts in the GV-vit group survived re-vitrification. Hence, after blastocyst re-vitrification, 8.2% of the vitrified oocytes re-expanded to transferable embryos. In conclusion, re-vitrification at the PN stage was detrimental for subsequent development, whereas that at the blastocyst stage was more advantageous.\u0000 </div>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145032529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

L-Carnitine Modulates Oocyte Development and Cumulus Expansion in Tropical Ruminants: Experimental and Bioinformatics Insights 左旋肉碱调节热带反刍动物卵母细胞发育和积云扩张：实验和生物信息学见解。

IF 1.2 3区农林科学

Animal Science Journal Pub Date : 2025-09-10 DOI: 10.1111/asj.70102

Mst Mamata Akter, Tajnin Jahan Tazi, Md Hasanur Alam, Ireen Akter, Mohammad Moniruzzaman

{"title":"L-Carnitine Modulates Oocyte Development and Cumulus Expansion in Tropical Ruminants: Experimental and Bioinformatics Insights","authors":"Mst Mamata Akter, Tajnin Jahan Tazi, Md Hasanur Alam, Ireen Akter, Mohammad Moniruzzaman","doi":"10.1111/asj.70102","DOIUrl":"10.1111/asj.70102","url":null,"abstract":"<div>\u0000 \u0000 This study investigates the effects of L-carnitine on nuclear maturation and fertilization in cattle and goat oocytes. Ovaries were collected from females with poor reproductive efficiency in the tropical climate, and cumulus-oocyte complexes (COCs) were retrieved from large antral follicles. COCs were cultured with varying concentrations of L-carnitine (0, 0.5, 1, and 1.5 mg/mL for goats; 0, 0.25, 0.375, and 0.5 mg/mL for cattle). Cumulus expansion and nuclear maturation were assessed, while bovine oocytes underwent in vitro fertilization. Here, L-carnitine enhanced cumulus expansion and nuclear maturation, with the highest maturation rates at 1 mg/mL in goats and 0.375 and 0.5 mg/mL in cattle. Fertilization rates in cattle improved with L-carnitine supplementation, particularly at 0.375 and 0.5 mg/mL. Gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses revealed that L-carnitine modulates pathways related to oxidative stress reduction, glucose metabolism, and mitochondrial function. Protein–protein interaction network analysis identified key regulatory genes, including SOD2, SIRT3, IGFBP3, PRL, NOS2, NOX4, SOD1, HMOX1, LEP, and AKT1, which may mediate L-carnitine's effects on oocyte maturation and fertilization. In conclusion, L-carnitine supplementation enhances oocyte competence possibly by improving cellular metabolism and reducing oxidative stress, providing valuable insights for optimizing assisted reproductive technologies in ruminants.\u0000 </div>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145032578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating Amino Acid Profiles and Blood Gas Concentrations Between Single and Twin Merino Newborn Lambs 单羔和双羔美利奴新生羔羊氨基酸谱和血气浓度的测定。

IF 1.2 3区农林科学

Animal Science Journal Pub Date : 2025-09-10 DOI: 10.1111/asj.70107

Leesa-Joy Dunstan, Michelle L. Hebart, Forbes D. Brien, Sue A. McCoard, Mariana Caetano

{"title":"Evaluating Amino Acid Profiles and Blood Gas Concentrations Between Single and Twin Merino Newborn Lambs","authors":"Leesa-Joy Dunstan, Michelle L. Hebart, Forbes D. Brien, Sue A. McCoard, Mariana Caetano","doi":"10.1111/asj.70107","DOIUrl":"10.1111/asj.70107","url":null,"abstract":"As sheep production standards progress, and animals are bred for high production in terms of the number and weight of lambs weaned per ewe, research has identified a difference in the physiology of single lambs compared to multiple born lambs. The current study aimed to report the baseline amino acid (AA) profiles and blood gas concentrations in newborn, Merino single and twin lambs. From 120 days of gestation, 50 single-bearing and 50 twin-bearing, naturally mated Merino ewes were monitored for signs of approaching parturition. At birth, blood samples of the progeny were collected, and birth weight, rectal temperature, and meconium score were recorded. Blood plasma samples were analysed for AA profiles and blood gas concentrations were determined using an i-Stat Alinity. Single-born lambs had a higher birth weight (5.05 kg) compared to twins (4.24 kg; p < 0.05). Birth rank also affected rectal temperature and AAs aspartic acid, isoleucine, leucine, and phenylalanine, all being lower in twins compared to singles (p < 0.05). These baseline data provide insight into the physiological differences between single and twin lambs at birth from dams where there has been no treatment or intervention imposed.","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/asj.70107","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145032597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Estimating the Textural Properties of Bromelain-Tenderized Beef by Impedance Measurement 用阻抗法评价菠萝蛋白酶嫩化牛肉的结构特性。

IF 1.2 3区农林科学

Animal Science Journal Pub Date : 2025-09-10 DOI: 10.1111/asj.70104

Miku Matsumoto, Akari Igarashi, Takayuki Muramoto

{"title":"Estimating the Textural Properties of Bromelain-Tenderized Beef by Impedance Measurement","authors":"Miku Matsumoto, Akari Igarashi, Takayuki Muramoto","doi":"10.1111/asj.70104","DOIUrl":"10.1111/asj.70104","url":null,"abstract":"Hardness of meat is one of the most important textural properties noted while eating. Bromelain, found in pineapples, is an enzyme that degrades collagen, a factor that affects meat hardness. The latter is generally evaluated based on shear strength and texture; however, such methods are destructive. This study aimed to develop a nondestructive impedance measurement method. We conducted impedance measurements, using touch-type electrodes, on the muscles in beef artificially tenderized with bromelain, and examined the relationship between textural properties and impedance to develop a method for estimating the textural properties of bromelain-tenderized beef by impedance measurement. The maximum load was significantly lower (p < 0.01) in the bromelain group than that in the others. Significant positive correlations (p < 0.01) were found between the total loss and impedance at all frequencies, and significant negative correlations were found between the maximum load and impedance at 120 Hz (p < 0.05), 20 kHz (p < 0.01), and 100 kHz (p < 0.05). The results of this study indicated that impedance measurements can be used to nondestructively estimate the water-holding capacity and hardness of beef tenderized by bromelain solution.","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/asj.70104","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145032580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Walnut (Juglans regia L.) Green Husk Supplemented to Total Mixture Ration on In Vitro Rumen Fermentation Parameters and Microbial Protein Synthesis 核桃的作用总混合日粮中添加青皮对体外瘤胃发酵参数及微生物蛋白合成的影响

IF 1.2 3区农林科学

Animal Science Journal Pub Date : 2025-09-10 DOI: 10.1111/asj.70110

Mazlum Mahsum Aslan, Çağrı Kale

{"title":"Effects of Walnut (Juglans regia L.) Green Husk Supplemented to Total Mixture Ration on In Vitro Rumen Fermentation Parameters and Microbial Protein Synthesis","authors":"Mazlum Mahsum Aslan, Çağrı Kale","doi":"10.1111/asj.70110","DOIUrl":"https://doi.org/10.1111/asj.70110","url":null,"abstract":"<div>\u0000 \u0000 The aim of this experiment was to determine the effects of walnut (Juglans regia L.) green husk (WGH) supplemented to ration on rumen fermentation by in vitro gas production technique. WGH was supplemented at different ratios (0%, 2%, 4%, 6%, 8%, and 10%) to the total mixture ration formed from 80%/20% roughage/concentrate feed. Experimental rations were incubated with 1/2 rumen fluid/artificial saliva mixture in in vitro gas production syringes in a water bath at 39°C ± 1°C for 24 h. WGH supplemented to the ration at different ratios increased in vitro organic matter digestibility (IVOMD), gas production (GP), ruminal ammonia nitrogen (NH3-N), and pH values linearly and quadratically. Methane ratio in total gas, metabolizable energy (ME) value, true digestibility (TD), and microbial protein (MP) synthesis also increased quadratically. With the supplementation of WGH to the ration, acetic acid (AA) concentration decreased linearly, while acetic acid/propionic acid ratio (AA/PA) decreased both linearly and quadratically. Overall, it was observed that WGH had positive effects on many rumen fermentation parameters. It is thought that the use of WGH in ruminant ration should be supported by new experiments with different supplementation ratios or ration mixtures.\u0000 </div>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145022135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating the Effects of Different n-6 Polyunsaturated Fatty Acid Ratios on Ferroptosis in IPEC-J2 Cells 不同n-6多不饱和脂肪酸比例对IPEC-J2细胞凋亡影响的研究

IF 1.2 3区农林科学

Animal Science Journal Pub Date : 2025-09-08 DOI: 10.1111/asj.70109

Ching-Hsuan Liu, Yuan-Yu Lin

{"title":"Investigating the Effects of Different n-6 Polyunsaturated Fatty Acid Ratios on Ferroptosis in IPEC-J2 Cells","authors":"Ching-Hsuan Liu, Yuan-Yu Lin","doi":"10.1111/asj.70109","DOIUrl":"https://doi.org/10.1111/asj.70109","url":null,"abstract":"<div>\u0000 \u0000 Dietary n-6 and n-3 polyunsaturated fatty acid (PUFA) balance critically modulates various physiological processes, including inflammation and cell death. This study investigated the effects of different n-6 PUFA ratios (1:1, 5:1, 10:1, 20:1) on ferroptosis in porcine IPEC-J2 intestinal epithelial cells. Cells treated with varying PUFA ratios showed a significant reduction in cell viability, which was alleviated by the ferroptosis inhibitor ferrostatin-1 (fer-1). The 20:1 n-6 PUFA ratio exhibited the most pronounced decrease in cell viability and the greatest increase in malondialdehyde (MDA) levels, a marker of lipid peroxidation. Levels of 4-hydroxynonenal (4-HNE), total iron, and proinflammatory cytokine IL-6 were significantly elevated in the 5:1, 10:1, and 20:1 groups, with fer-1 treatment mitigating these effects. The GSSG/GSH ratio, an indicator of oxidative stress, also significantly increased in the 10:1 and 20:1 groups. These findings suggest that higher n-6 PUFA content exacerbates lipid peroxidation, iron accumulation, and inflammatory responses, leading to ferroptosis in IPEC-J2 cells. This study highlights the importance of dietary n-6 PUFA balance in modulating ferroptosis and intestinal health, offering potential insights into strategies for improving intestinal function by regulating ferroptosis pathways.\u0000 </div>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145012193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Environment- and Animal-Based Factors on the Main Components and Bioactive Substances in Buffalo Colostrum and Calf Serum 环境和动物性因素对水牛初乳和犊牛血清主要成分和生物活性物质的影响

IF 1.2 3区农林科学

Animal Science Journal Pub Date : 2025-09-08 DOI: 10.1111/asj.70108

İbrahim Cihangir Okuyucu, Hüseyin Erdem

{"title":"Effect of Environment- and Animal-Based Factors on the Main Components and Bioactive Substances in Buffalo Colostrum and Calf Serum","authors":"İbrahim Cihangir Okuyucu, Hüseyin Erdem","doi":"10.1111/asj.70108","DOIUrl":"https://doi.org/10.1111/asj.70108","url":null,"abstract":"<div>\u0000 \u0000 This study aimed to evaluate the effect of some environment- and animal-based factors, such as body condition score (BCS) on 15–20 days before parturition, parity, and calving season on colostrum dry matter (DM), fat, solids-nonfat (SNF), protein, and lactose contents as well as colostrum and calf's serum immunoglobulin (IgG, IgA, and IgM), IGF-1, and lactoferrin concentrations in buffaloes. Therefore, the components of colostrum (at the first milking) and calf serum samples (at 24–48 h and 28 days after birth) from 86 Anatolian buffalo cows were analyzed by an infrared milk analyzer and ELISA test. The high BCS enhanced colostrum DM, fat, and IgG content; calf serum IgG concentration at 24–48 h, and lactoferrin at 28 days compared to low BCS. At 28 days, the serum IgA and IgM concentrations were higher in the calves of cows with a parity of 2 and 4, respectively, compared to other parities. The colostrum SNF and protein percentage in summer were higher than that in autumn but not in winter and spring. In conclusion, a high BCS could ensure successful passive immunity transfer in buffalo calves by improving the studied colostrum and calf serum parameters, which are affected by parity and calving season.\u0000 </div>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145012115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Utilizing the Laparoscopic Ovum Pick-Up Method for In Vitro Embryo Production in Prepubertal Anatolian Native Black Calves: A Promising Approach for Gene Banks and Endangered Species 利用腹腔镜取卵法在安纳托利亚原生黑犊的青春期前体外胚胎生产：基因库和濒危物种的一种有前途的方法

IF 1.2 3区农林科学

Animal Science Journal Pub Date : 2025-09-03 DOI: 10.1111/asj.70099

Ramazan Sevgi, Alaeddin Okuroğlu, Mehmet Ali Yılmaz, Cemal Dayanıklı, Derya Şahin, Seher Şimşek, Ali Şimşek, Burak Artut, Yasin Ergiden, İlker Ünal, Aslıhan Turhan

{"title":"Utilizing the Laparoscopic Ovum Pick-Up Method for In Vitro Embryo Production in Prepubertal Anatolian Native Black Calves: A Promising Approach for Gene Banks and Endangered Species","authors":"Ramazan Sevgi, Alaeddin Okuroğlu, Mehmet Ali Yılmaz, Cemal Dayanıklı, Derya Şahin, Seher Şimşek, Ali Şimşek, Burak Artut, Yasin Ergiden, İlker Ünal, Aslıhan Turhan","doi":"10.1111/asj.70099","DOIUrl":"https://doi.org/10.1111/asj.70099","url":null,"abstract":"<div>\u0000 \u0000 Recent advancements in genomic technology have increased interest in using calves as oocyte sources through the laparoscopic ovum pick-up (LOPU) method, which shortens generation intervals and enhances genetic gain. This study aimed to produce embryos in vitro using oocytes from endangered Anatolian Native Black calves and to increase the number of embryos in the gene bank through LOPU, a method not previously studied in Turkey. Four Anatolian Native Black calves, approximately 3 months old, were used. The super stimulation protocol involved the application of an intravaginal sponge starting on day −5 and continuing until the LOPU procedure. FSH was administered intramuscularly six times at 12-h intervals starting at −72 h, with laparoscopic oocyte aspiration on day 0. A total of 42 A-B quality oocytes were collected, averaging 10.5 per calf. Maturation completion was 88.09%, with a cleavage rate of 76.19% at 24 h after fertilization. On day 7, blastocyst success was 28.57%. The study demonstrates that LOPU can successfully produce in vitro embryos in prepubertal endangered breed calves in Turkey, proving that LOPU is an effective method for gene bank embryo production.\u0000 </div>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144934821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0