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Effects of Crimson Clover Silage Inclusion on Nutrient Intake, Behavior, and Stress Condition in Gestating Sows 苜蓿青贮包埋对妊娠母猪营养摄入、行为和应激状态的影响
IF 1.7 3区 农林科学
Animal Science Journal Pub Date : 2025-06-08 DOI: 10.1111/asj.70065
Andrew L. Makalani, Yasuyuki Ishii, Tomoyuki Kawashima, Ikuo Kobayashi, Toshihiro Takahashi
{"title":"Effects of Crimson Clover Silage Inclusion on Nutrient Intake, Behavior, and Stress Condition in Gestating Sows","authors":"Andrew L. Makalani,&nbsp;Yasuyuki Ishii,&nbsp;Tomoyuki Kawashima,&nbsp;Ikuo Kobayashi,&nbsp;Toshihiro Takahashi","doi":"10.1111/asj.70065","DOIUrl":"https://doi.org/10.1111/asj.70065","url":null,"abstract":"<div>\u0000 \u0000 <p>This study aimed to determine whether crimson clover silage (CSS) can serve as an effective and beneficial substitute for conventional sow diets, to enhance satiety and normal behavior, while reducing production costs. Six sows with an average initial body weight (BW) of 246.0 ± 16.0 kg and an initial backfat thickness of 23.8 ± 1.10 mm were assigned to a 6 × 2 crossover design. Each feeding period included a 10-day acclimation period and a 10-day data collection period for each treatment. In the test treatment, a commercial diet was replaced by CCS on a dry matter (DM) basis at 20%, while the control treatment had no CCS inclusion. Data on daily feed intake, BW, and backfat thickness were recorded, and urine samples for cortisol analysis and video footage for behavior analysis were collected on the last day of each treatment. There were no leftovers with either diet, and nutrient uptake, BW, and backfat thickness were unaffected (<i>p</i> &gt; 0.05). However, stereotypic behaviors and urinary cortisol concentrations were significantly reduced (<i>p</i> &lt; 0.05) with CCS inclusion. Replacing 20% DM of the commercial diet with crimson clover silage presents an effective strategy for lowering feeding costs while also enhancing animal behavior and well-being.</p>\u0000 </div>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144244530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Enhanced Retinol Extraction From Bovine Whole Blood Using n-Hexane and Phosphate-Buffered Saline 正己烷和磷酸盐缓冲盐水强化牛全血视黄醇提取
IF 1.7 3区 农林科学
Animal Science Journal Pub Date : 2025-06-05 DOI: 10.1111/asj.70069
Wataru Iwasaki, Nobutomo Morita, Risa Yokoishi, Tetsuro Inoue, Takanori Jogi, Seiyu Miki
{"title":"Enhanced Retinol Extraction From Bovine Whole Blood Using n-Hexane and Phosphate-Buffered Saline","authors":"Wataru Iwasaki,&nbsp;Nobutomo Morita,&nbsp;Risa Yokoishi,&nbsp;Tetsuro Inoue,&nbsp;Takanori Jogi,&nbsp;Seiyu Miki","doi":"10.1111/asj.70069","DOIUrl":"https://doi.org/10.1111/asj.70069","url":null,"abstract":"<p>Ensuring optimal blood retinol levels of cattle during fattening is essential for achieving high-quality marbling in beef production. We previously developed a rapid analytical method for measuring blood retinol concentration that eliminates the process of blood cell separation. However, achieving a strong correlation with actual retinol levels proved challenging due to the requirements of vigorous agitation of blood and <i>n</i>-hexane using a vortex mixer. Consequently, in this study, we developed a modified method that does not require intense agitation by adding phosphate-buffered saline (PBS) to the blood and <i>n</i>-hexane mixture. The addition of PBS facilitated the dispersion of whole blood in <i>n</i>-hexane, although the augmented interface between the two enhanced extraction efficiency. Furthermore, we conducted field tests using blood samples collected from 98 fattening cattle from six different farms, each using distinct feeding methods and rearing environments, to reflect the diverse feeding practices in Japanese Black beef production. Their retinol concentrations were analyzed using the developed method and compared with values obtained by HPLC. A correlation coefficient of 0.94–0.96 was obtained, indicating that blood retinol levels can be reliably measured even among farms with different feeding methods.</p>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/asj.70069","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144220128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dietary Supplementation With Mannitol Regulates the Rumen and Intestinal Microbiome, Immune Function, and Antioxidant Capacity of Grazed Mongolian Sheep 饲粮中添加甘露醇对放牧蒙古羊瘤胃和肠道微生物群、免疫功能和抗氧化能力的调节
IF 1.7 3区 农林科学
Animal Science Journal Pub Date : 2025-06-03 DOI: 10.1111/asj.70074
Xiao Min,  Aldar, Gong Jian
{"title":"Dietary Supplementation With Mannitol Regulates the Rumen and Intestinal Microbiome, Immune Function, and Antioxidant Capacity of Grazed Mongolian Sheep","authors":"Xiao Min,&nbsp; Aldar,&nbsp;Gong Jian","doi":"10.1111/asj.70074","DOIUrl":"https://doi.org/10.1111/asj.70074","url":null,"abstract":"<div>\u0000 \u0000 <p>This study aimed to investigate the short-term effects of mannitol supplementation on grazing Mongolian sheep, including ruminal and intestinal microbiome, serum biochemical parameters, and immune and antioxidant functions. Ten sheep were randomly divided into control and experimental groups (<i>n</i> = 5) receiving without or with 10 g/kg mannitol, respectively. After 20 days, including a 5-day adaptation, mannitol did not affect the average daily gain and rumen fermentation but significantly altered microbial composition, as evidenced by principal coordinates' analysis. The abundances of genera including <i>Rikenellaceae_RC9_gut_group</i> increased notably in the rumen, and those of <i>Alistipes</i> and <i>Norank_f_Muribaculaceae</i> increased in intestine with mannitol supplementation. Mannitol increased the serum concentrations of total protein, albumin, retinol-binding protein, paraoxonase, and total cholesterol and decreased alanine aminotransferase levels. Mannitol also increased the levels of immunoglobulins, interleukins, tumor necrosis factor, interferon, and complements and antioxidant enzyme activities while reducing malondialdehyde and hydrogen peroxide concentrations. The serum total protein positively correlated with <i>Rikenellaceae_RC9_gut_group</i> and <i>Alistipes</i> while negatively correlated with superoxide dismutase and specific microbial genera. Taken together, mannitol supplementation reshapes ruminal and intestinal microbial communities, improving serum biomarkers, immune responses, and antioxidant defenses in grazing Mongolian sheep. These findings offer insights into novel feeding strategies for the management of grazing sheep.</p>\u0000 </div>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144206386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of Various Factors, Including Management Type at the Slaughterhouse, on the Presence of Dark Cutting in Cattle: A Study in Turkey 包括屠宰场管理类型在内的各种因素对牛黑切现象的影响:土耳其的一项研究
IF 1.7 3区 农林科学
Animal Science Journal Pub Date : 2025-06-03 DOI: 10.1111/asj.70073
Seyfi Özdemir, Bulent Ekiz
{"title":"Effects of Various Factors, Including Management Type at the Slaughterhouse, on the Presence of Dark Cutting in Cattle: A Study in Turkey","authors":"Seyfi Özdemir,&nbsp;Bulent Ekiz","doi":"10.1111/asj.70073","DOIUrl":"https://doi.org/10.1111/asj.70073","url":null,"abstract":"<p>The aim of this study was to examine the effects of various factors, especially the management type applied to cattle in the slaughterhouse, on meat pH<sub>24h</sub> and the presence of dark cutting. Data from 583 cattle of the Holstein and Simmental breeds were evaluated. Cattle were divided into four management types according to whether they were tethered or nontethered in the unloading, lairage, and slaughter corridor. Mean pH<sub>24h</sub> was 5.60, and the dark-cutting percentage was 13.20%. The effect of management type on the presence of dark cutting was significant (<i>p</i> = 0.047). The mean pH<sub>24h</sub> value was higher in cattle from the nontethered management group (G-I, 5.642) than in cattle from the tethered group (G-II, 5.579). The presence of dark cutting was 2.519 times higher in the G-I cattle than in the cattle in Group IV (tethered during unloading and at the lairage and nontethered at the slaughter corridor). The effects of season, sex, season × sex, season × management type, sex × management type, age, trackload density, and carcass weight on pH<sub>24h</sub> were significant. In conclusion, the tethered management type may contribute to reducing the presence of dark cutting in slaughterhouses that were established without considering animal behaviors and ease of cattle handling.</p>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/asj.70073","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144206385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Association of Somatic Cell Score at First Calving With Milk Productivity and Mortality in Dairy Herds: A Cross-Sectional Study in Eastern Hokkaido, Japan 日本北海道东部奶牛群初产犊时体细胞评分与产奶量和死亡率的相关性研究
IF 1.7 3区 农林科学
Animal Science Journal Pub Date : 2025-06-02 DOI: 10.1111/asj.70071
Akira Goto, Mitsuo Yokoi, Naoki Yamamoto, Yoichi Inoue, Keiichi Hisaeda, Yasunori Shinozuka, Ken Nakada
{"title":"Association of Somatic Cell Score at First Calving With Milk Productivity and Mortality in Dairy Herds: A Cross-Sectional Study in Eastern Hokkaido, Japan","authors":"Akira Goto,&nbsp;Mitsuo Yokoi,&nbsp;Naoki Yamamoto,&nbsp;Yoichi Inoue,&nbsp;Keiichi Hisaeda,&nbsp;Yasunori Shinozuka,&nbsp;Ken Nakada","doi":"10.1111/asj.70071","DOIUrl":"https://doi.org/10.1111/asj.70071","url":null,"abstract":"<div>\u0000 \u0000 <p>Over the past decade, the increasing proportion of primiparous cows in Hokkaido dairy herds has resulted in younger herds, but somatic cell count (SCC) has stagnated at around 220,000 cells/mL, preventing milk quality improvements. This study evaluated the impact of elevated postpartum somatic cell scores (SCS) in primiparous cows on herd productivity, aiming to inform milk quality improvement measures in Hokkaido. Production data from 163 herds in Eastern Hokkaido were analyzed, with milk yield per cow, herd SCC, and mortality used as dependent variables and primiparous cow SCS as the independent variable. Results revealed a significant positive association between primiparous cow SCS and both herd SCC and mortality, whereas a significant negative association was observed with milk yield per cow. These findings suggest that high SCS in primiparous cows may reflect suboptimal animal welfare practices, such as poor udder hygiene and inadequate peripartum management. Enhancing welfare practices, such as udder hygiene, and providing a supportive calving environment could improve SCS in primiparous cows, increase milk productivity, and reduce mortality. Additionally, supporting farmers by using SCC levels of primiparous cows as a benchmark could be an effective strategy for improving milk quality in the Hokkaido region.</p>\u0000 </div>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144197459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Equol-Producing Bacteria in the Chicken Intestine: PCR Analysis on the Bacteria Quantitatively Correlating With Equol Concentration in Daidzein-Fed Laying Hens 鸡肠内产马酚细菌:用大豆素饲养的蛋鸡体内马酚浓度与细菌数量相关的PCR分析
IF 1.7 3区 农林科学
Animal Science Journal Pub Date : 2025-05-31 DOI: 10.1111/asj.70068
Sota Sakai, Kyohei Furukawa, Fu Namai, Keita Nishiyama, Dipson Gyawali, Tatsuhiko Goto, Motoi Kikusato
{"title":"Equol-Producing Bacteria in the Chicken Intestine: PCR Analysis on the Bacteria Quantitatively Correlating With Equol Concentration in Daidzein-Fed Laying Hens","authors":"Sota Sakai,&nbsp;Kyohei Furukawa,&nbsp;Fu Namai,&nbsp;Keita Nishiyama,&nbsp;Dipson Gyawali,&nbsp;Tatsuhiko Goto,&nbsp;Motoi Kikusato","doi":"10.1111/asj.70068","DOIUrl":"https://doi.org/10.1111/asj.70068","url":null,"abstract":"<div>\u0000 \u0000 <p>Equol is a microbial metabolite from daidzein, an isoflavone, in the animal intestine. This study aimed to identify equol-producing bacteria in the intestines of chickens. Sixteen 181-day-old Hy-Line Brown laying hens were given a basal diet formulated excluding soy products for 2 weeks and subsequently given the diets supplemented with 0-, 100-, 1000-, and 10,000-ppm daidzein for 10 days. Plasma and egg yolk equol concentration was significantly increased with dietary daidzein supplementation. Gene abundance of two major bacterial enzymes catalyzing the conversion of daidzein to equol, dihydrodaidzein reductase, and tetrahydro daidzein reductase was measured using polymerase chain reaction (PCR) analysis, with the abundance increasing with dietary daidzein supplementation (<i>p</i> &lt; 0.05). After sequencing the PCR amplicons, six possible bacteria were identified, and they were further identified and quantified using a specific primer for each bacterial gene. As a result, <i>Adlercreutzia equolifaciens</i> and <i>Slackia equolifaciens</i> were identified, and their bacterial relative abundance increased with dietary daidzein concentration and plasma equol levels (<i>p</i> &lt; 0.05). <i>A. equolifaciens</i> and <i>S. equolifaciens</i> are known as equol-producing bacteria, and the present study was the first to show that these bacteria could also contribute to equol production in chickens.</p>\u0000 </div>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144179250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Improvement of Sperm Quality in Cryopreserved Boar Semen by Supplementation of Kaempferia parviflora and Cholesterol-Loaded Cyclodextrin in Semen Extender 增精剂中添加山柰和高胆固醇环糊精对猪冷冻精液精子质量的改善
IF 1.7 3区 农林科学
Animal Science Journal Pub Date : 2025-05-28 DOI: 10.1111/asj.70066
Himalai Saiyamanon, Ruthaiporn Ratchamak, Khomsan Buathalad, Wuttigrai Boonkum, Vibuntita Chankitisakul
{"title":"Improvement of Sperm Quality in Cryopreserved Boar Semen by Supplementation of Kaempferia parviflora and Cholesterol-Loaded Cyclodextrin in Semen Extender","authors":"Himalai Saiyamanon,&nbsp;Ruthaiporn Ratchamak,&nbsp;Khomsan Buathalad,&nbsp;Wuttigrai Boonkum,&nbsp;Vibuntita Chankitisakul","doi":"10.1111/asj.70066","DOIUrl":"https://doi.org/10.1111/asj.70066","url":null,"abstract":"<div>\u0000 \u0000 <p>We investigated the potential of <i>Kaempferia parviflora</i> <b>(</b>KP) extract, rich in phenolic compounds, for enhancing boar semen quality during cryopreservation. We evaluated the impact of various KP extract concentrations (0%–8% w/v) in freezing extenders on postthawed semen quality and oxidative stress markers, along with the effects of cholesterol-loaded cyclodextrins (CLC). Semen was frozen using liquid nitrogen vapor before being plunged into liquid nitrogen, and postthaw semen quality and lipid peroxidation were analyzed. Results showed that 2%–4% KP extract significantly improved total and progressive motility, sperm viability, and acrosome integrity compared to the control (<i>p</i> &lt; 0.05), likely due to reduced lipid peroxidation indicated by lower malondialdehyde (MDA) levels (<i>p</i> &lt; 0.05). However, higher concentrations (6%–8%) negatively affected sperm quality. Adding CLC further enhanced the positive effects of KP extract, improving sperm motility and acrosome integrity and reducing lipid peroxidation (<i>p</i> &lt; 0.05). In conclusion, supplementation of boar semen cryopreservation media with 2% KP extract significantly enhanced sperm quality postthawing, likely through its potent antioxidant properties. Combining KP extract with CLC amplified these beneficial effects. These findings highlight a promising natural strategy for improving boar semen cryopreservation outcomes.</p>\u0000 </div>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144171206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of Microplastic Contamination on In Vitro Ruminal Fermentation and Feed Degradability 微塑料污染对体外瘤胃发酵及饲料降解率的影响
IF 1.7 3区 农林科学
Animal Science Journal Pub Date : 2025-05-27 DOI: 10.1111/asj.70063
Khalil Abid, Mohamed Aroua, Salvatore Barbera, Sara Glorio Patrucco, Hatsumi Kaihara, Mokhtar Mahouachi, Samia Ben Saïd, Sonia Tassone
{"title":"Effect of Microplastic Contamination on In Vitro Ruminal Fermentation and Feed Degradability","authors":"Khalil Abid,&nbsp;Mohamed Aroua,&nbsp;Salvatore Barbera,&nbsp;Sara Glorio Patrucco,&nbsp;Hatsumi Kaihara,&nbsp;Mokhtar Mahouachi,&nbsp;Samia Ben Saïd,&nbsp;Sonia Tassone","doi":"10.1111/asj.70063","DOIUrl":"https://doi.org/10.1111/asj.70063","url":null,"abstract":"<p>This study examined the effects of microplastic (MP) contamination on rumen fermentation dynamics and concentrate degradability using an in vitro model with lamb rumen fluid. Three types of MPs—polyethylene terephthalate (PET), low-density polyethylene (LDPE), and polyamide (PA)—were tested at contamination levels of 0%, 0.6%, 1.2%, and 1.8% of dry matter. MP contamination significantly disrupted rumen fermentation dynamics, reduced feed degradability, increased gas production, accelerated fermentation rates, and shortened the lag time before gas production (<i>p</i> &lt; 0.05). Additionally, MPs impaired microbial efficiency, increased ammonia-nitrogen (NH₃-N) levels, decreased rumen protozoa populations, and reduced concentrate degradability (<i>p</i> &lt; 0.05). LDPE exhibited the most severe effects, causing the highest increases in gas production and NH₃-N levels (15% and 12%, respectively at LDPE highest dose) while decreasing microbial efficiency, protozoa count, and feed degradability (16.0%, 16.4%, and 4.5%, respectively at LDPE highest dose). The severity of MPs' impacts followed a significant linear trend, with higher concentrations leading to more pronounced negative effects. The findings highlight MPs as significant emerging pollutants that can adversely affect rumen function and animal nutrition.</p>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/asj.70063","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144148571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Resveratrol Supplementation in In Vitro Maturation and Culture Medium: Enhancing Blastocyst Viability After Vitrification 在体外成熟和培养基中添加白藜芦醇:提高玻璃化后囊胚活力
IF 1.7 3区 农林科学
Animal Science Journal Pub Date : 2025-05-15 DOI: 10.1111/asj.70061
Kamolchanok Tonekam, Yanapon Anthakat, Apisit Polrachom, Worawalan Samruan, Preeyanan Anwised, Pakpoom Boonchuen, Mariena Ketudat-Cairns, Rangsun Parnpai
{"title":"Resveratrol Supplementation in In Vitro Maturation and Culture Medium: Enhancing Blastocyst Viability After Vitrification","authors":"Kamolchanok Tonekam,&nbsp;Yanapon Anthakat,&nbsp;Apisit Polrachom,&nbsp;Worawalan Samruan,&nbsp;Preeyanan Anwised,&nbsp;Pakpoom Boonchuen,&nbsp;Mariena Ketudat-Cairns,&nbsp;Rangsun Parnpai","doi":"10.1111/asj.70061","DOIUrl":"https://doi.org/10.1111/asj.70061","url":null,"abstract":"<div>\u0000 \u0000 <p>Resveratrol is a potent antioxidant that improves the quality, development, and freezing resistance of mammalian oocytes and embryos by reducing oxidative stress. This study examined the effects of resveratrol supplementation during in vitro maturation (IVM), in vitro culture (IVC), and post-vitrification on the developmental competence of bovine embryos derived from vitrified blastocysts. Oocytes were matured in media with resveratrol at concentrations of 0, 0.5, 1.0, and 2.0 μM. Mitochondrial analysis indicated that vitrified oocytes matured with resveratrol exhibited significantly higher mitochondrial activity than controls (<i>p</i> &lt; 0.05). In IVC, zygotes treated with 0.5 μM resveratrol exhibited significantly higher cleavage (81.70%) and blastocyst rates (37.75%) than untreated embryos (<i>p</i> &lt; 0.05). Vitrified blastocysts derived from resveratrol-supplemented IVC and subsequently cultured without resveratrol for 48 h showed the highest development to the hatching and hatched blastocyst stage (64.15%). Gene expression analysis revealed the <i>BCL2</i> and <i>SIRT1</i> upregulation and <i>BAX</i> downregulation. These findings suggest that resveratrol enhances oocyte and embryo quality, mitochondrial function, and cryotolerance.</p>\u0000 </div>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143949927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
High Dietary Cation and Anion Difference Enhanced Renal Response to an Acute Acid Load of Lactating Goat Fed Under Tropical Conditions 热带条件下高阴阳离子饲粮差异增强了哺乳山羊对急性酸负荷的肾脏反应
IF 1.7 3区 农林科学
Animal Science Journal Pub Date : 2025-05-10 DOI: 10.1111/asj.70062
Sapon Semsirmboon, Dang Khoa do Nguyen, Narongsak Chaiyabutr, Sutthasinee Poonyachoti, Thomas A. Lutz, Sumpun Thammacharoen
{"title":"High Dietary Cation and Anion Difference Enhanced Renal Response to an Acute Acid Load of Lactating Goat Fed Under Tropical Conditions","authors":"Sapon Semsirmboon,&nbsp;Dang Khoa do Nguyen,&nbsp;Narongsak Chaiyabutr,&nbsp;Sutthasinee Poonyachoti,&nbsp;Thomas A. Lutz,&nbsp;Sumpun Thammacharoen","doi":"10.1111/asj.70062","DOIUrl":"https://doi.org/10.1111/asj.70062","url":null,"abstract":"<div>\u0000 \u0000 <p>High ambient temperature (HTa) in tropical conditions leads to evaporative heat dissipation by panting in lactating dairy goats. This panting causes respiratory hypocapnia, which increases the renal excretion of buffer molecules including sodium, potassium, and bicarbonate. In response, renal acid excretion decreases to preserve acidity. Besides hypocapnia, lactation and HTa could further deplete buffer molecules, enhancing acid preservation. This current study restored buffer molecules using high dietary cation and anion difference (DCAD) regimen and hypothesized that renal acid excretion during acute acid load would be increased in dairy goats fed high DCAD under HTa. To investigate this, 12 early-lactating dairy goats were randomly assigned to two groups: control and DCAD, which were fed control and high-DCAD diets, respectively, for 8 weeks. Acute acid loading during HTa was induced by high-dose ascorbic acid (AA) supplementation. During Weeks 4 and 8, the vehicle and AA were supplemented on the last two consecutive days of each week. The results showed that the renal acid excretion in DCAD group was higher than control group during high-dose AA supplementation. Together with high renal buffer excretion by DCAD, an increased renal acid excretion may be partially mediated by an increase in buffer reserve.</p>\u0000 </div>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143930515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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