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Evaluation of Fermentation Quality, Digestive Characteristics, and Nutritive Value of Whole Crop Silage Prepared by Fine-Cutting From Rice Cultivars With Short Panicles and Fed to Dairy Cows. 短穗水稻精切全株青贮饲喂奶牛发酵品质、消化特性及营养价值评价
IF 1.6 3区 农林科学
Animal Science Journal Pub Date : 2026-01-01 DOI: 10.1111/asj.70240
Higuchi Kouji, Nonaka Itoko, Ohtani Fumihiro, Osawa Ryo
{"title":"Evaluation of Fermentation Quality, Digestive Characteristics, and Nutritive Value of Whole Crop Silage Prepared by Fine-Cutting From Rice Cultivars With Short Panicles and Fed to Dairy Cows.","authors":"Higuchi Kouji, Nonaka Itoko, Ohtani Fumihiro, Osawa Ryo","doi":"10.1111/asj.70240","DOIUrl":"10.1111/asj.70240","url":null,"abstract":"<p><p>Silage fermentation and digestibility characteristics, ruminal fermentation, and chewing activity in dairy cattle fed whole-crop rice silages having short panicle cultivars \"Tachisuzuka,\" \"Tachiayaka,\" and \"Tsukisuzuka\" were evaluated. All cultivars used in this study were grown under identical conditions, harvested using a fine-cutting harvesting machine, and ensiled in a round baler for the chopped material along with Lactobacillus inoculation. Four nonlactating, nonpregnant, rumen-cannulated Holstein cows were housed in a climate-controlled room and fed one of the three silage diets in a 3 × 3 Latin square design. Every 2 weeks, the cows were housed in respiration chambers for 5 days to measure gaseous exchange, energy and nitrogen balance, apparent total-tract digestibility of nutrients, and chewing activity. All cultivars showed fermentation quality with low pH and high acetic acid content. The digestibilities of fiber and nonfibrous carbohydrates were higher in Tachisuzuka and Tachiayaka than in the other cultivars, respectively. The total digestible nutrient contents in Tachisuzuka, Tachiayaka, and Tsukisuzuka were 61.4%, 60.8%, and 59.4%, respectively. The roughage value index was 79-86. The high digestibility of these silage cultivars suggests that they could be used as feed for lactating cows; however, their high chewing stimulus also needs to be considered.</p>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"97 1","pages":"e70240"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13545000/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148890901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Egg Quality Traits Among Four Japanese Indigenous Breeds of Chickens. 日本4个地方品种鸡的蛋品质特征。
IF 1.6 3区 农林科学
Animal Science Journal Pub Date : 2026-01-01 DOI: 10.1111/asj.70182
Emmanuel Tope Ogunwale, Dipson Gyawali, Tatsuhiko Goto
{"title":"Egg Quality Traits Among Four Japanese Indigenous Breeds of Chickens.","authors":"Emmanuel Tope Ogunwale, Dipson Gyawali, Tatsuhiko Goto","doi":"10.1111/asj.70182","DOIUrl":"10.1111/asj.70182","url":null,"abstract":"<p><p>Japanese indigenous chickens potentially produce good-quality eggs. This study aims to analyze egg quality traits using four Japanese indigenous breeds: Tosa-jidori (TSJ), Ko-Shamo (KSM), Minohikichabo (MHC), and Chabo (CHB). Eleven egg quality traits were measured from 73 hens in total. One-way ANOVA, correlation analysis, and principal component analysis (PCA) were conducted. Significant breed effects were observed for nine egg traits, except for the length of the short axis of the egg and eggshell yellowness. TSJ and KSM have significantly higher egg weight and albumen weight than MHC and CHB. The weights of yolk and eggshell in KSM were significantly higher than those of the others. MHC indicated significantly higher eggshell strength and thickness than CHB. MHC showed significantly redder eggshells than the others. PC1 (size and weight of egg components), PC2 (eggshell quality and color), and PC3 (thickness, weight, and redness of the eggshell) explained 76.9% of the total variance. Significant breed effects were observed for PC1 and PC2, revealing breed-specific characteristics in egg traits in this study. Moderate to strong correlations were found both among size and weight traits and among eggshell color traits. These results suggest the importance of characterizing untapped genetic resources in egg quality traits.</p>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"97 1","pages":"e70182"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147760176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Meta-Analysis of the Mitigation Effects of Supplementation With Calcium Salts of Unsaturated Fatty Acids on Enteric Methane Emissions in Beef Cattle and Its Impact on Meat Quality. 补充不饱和脂肪酸钙盐对肉牛肠道甲烷排放及其对肉质影响的meta分析
IF 1.6 3区 农林科学
Animal Science Journal Pub Date : 2026-01-01 DOI: 10.1111/asj.70239
Kazuki Matsubara, Tomoyuki Suzuki, Yuko Kamiya, Mikito Higuchi, Kazuhide Morimoto, Takahiro Fukuma, Shinichi Suenaga, Akihiro Nishikawa, Sachio Kohno, Shinji Oyama, Moe Kimura, Kazumi Hoshi, Jo Ezure, Jiro Imai, Satoshi Yamamoto
{"title":"Meta-Analysis of the Mitigation Effects of Supplementation With Calcium Salts of Unsaturated Fatty Acids on Enteric Methane Emissions in Beef Cattle and Its Impact on Meat Quality.","authors":"Kazuki Matsubara, Tomoyuki Suzuki, Yuko Kamiya, Mikito Higuchi, Kazuhide Morimoto, Takahiro Fukuma, Shinichi Suenaga, Akihiro Nishikawa, Sachio Kohno, Shinji Oyama, Moe Kimura, Kazumi Hoshi, Jo Ezure, Jiro Imai, Satoshi Yamamoto","doi":"10.1111/asj.70239","DOIUrl":"10.1111/asj.70239","url":null,"abstract":"<p><p>This study evaluated the effects of calcium salts of unsaturated fatty acids (CSUFAs) on enteric methane (CH<sub>4</sub>) emissions and meat quality in beef cattle by integrating data from trials on beef cattle of different production stages, sexes, and breeds. Specifically, a meta-analysis of four studies including 44 animals (22 with CSUFAs and 22 as a control) was performed to assess CH<sub>4</sub>-related outcomes. Daily CH<sub>4</sub> emissions in all experiments were estimated by multiplying the breath CH<sub>4</sub>:CO<sub>2</sub> concentration ratio by estimated CO<sub>2</sub> emissions. CSUFA supplementation tended to reduce CH<sub>4</sub> emissions per unit of total digestible nutrient intake (CH<sub>4</sub>/TDNI; mean difference = -2.2 L/kg, p = 0.077), corresponding to a reduction of 5.6%. Meanwhile, no significant differences were observed for the CH<sub>4</sub>:CO<sub>2</sub> ratio, daily CH<sub>4</sub> emissions, or CH<sub>4</sub> per unit of dry matter intake. Physicochemical analysis of meat from 16 animals revealed no adverse effects of CSUFA supplementation on meat quality. Descriptive sensory evaluation indicated improved juiciness, fat flavor richness, and other sensory attributes upon CSUFA supplementation (p < 0.05). These findings suggest that CSUFAs can reduce enteric CH<sub>4</sub> emissions relative to dietary energy density while maintaining meat quality, supporting their use as a practical strategy for mitigating CH<sub>4</sub> emissions from beef cattle.</p>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"97 1","pages":"e70239"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13548069/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148900087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparison of Temperature and Humidity Data Measured at Cattle Farm in Japan With Meteorological Observation Records and NASA POWER Data. 日本养牛场温度和湿度数据与气象观测记录和NASA POWER数据的比较。
IF 1.6 3区 农林科学
Animal Science Journal Pub Date : 2026-01-01 DOI: 10.1111/asj.70256
Shinichiro Ogawa, Yoichiro Hoshino, Norihide Yokoi
{"title":"Comparison of Temperature and Humidity Data Measured at Cattle Farm in Japan With Meteorological Observation Records and NASA POWER Data.","authors":"Shinichiro Ogawa, Yoichiro Hoshino, Norihide Yokoi","doi":"10.1111/asj.70256","DOIUrl":"https://doi.org/10.1111/asj.70256","url":null,"abstract":"<p><p>To evaluate the utility of open-access meteorological observation records to estimate on-farm thermal information, temperature and humidity data during June 5, 2024-April 15, 2026 were collected on the Kyoto University Livestock Farm in Japan. Data loggers were installed in breeding barn, calf barn, and paddock. Daily average dry-bulb temperature (T<sub>db</sub>), relative humidity (RH), volumetric humidity (VH), and temperature-humidity index (THI) were measured. Ambient temperature and humidity were estimated using meteorological observations from the two nearest weather stations. NASA POWER daily average T<sub>db</sub> and RH data at the location of farm were downloaded. Correlations of on-farm data were 0.86-0.91 for RH and > 0.99 for T<sub>db</sub>, VH, and THI with estimated values from meteorological observations, while 0.73-0.74 for RH, 0.99 for VH, and > 0.99 for T<sub>db</sub> and THI with NASA POWER data. Downward biases for T<sub>db</sub> and THI and upward biases for RH and VH were observed and the degree of biases for humidity-related indices tended to be more pronounced during the cold season, while bias in RH was greater for NASA POWER data. Our results could provide novel insight into thermal information on cattle farms and using public databases to infer on-farm temperature and humidity.</p>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"97 1","pages":"e70256"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149190524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Preliminary Study: High-Concentrate Feeding During the Growing Period Induces Region-Specific Growth and Alters Growth Hormone Dynamics in Japanese Black Heifers. 生长期高精料饲喂对日本黑母牛区域特异性生长和生长激素动态的影响。
IF 1.6 3区 农林科学
Animal Science Journal Pub Date : 2026-01-01 DOI: 10.1111/asj.70213
Taketo Haginouchi, Tomiko Fumita, Daichi Nishino, Yutaka Suzuki, Shotaro Nishimura, Takafumi Gotoh
{"title":"Preliminary Study: High-Concentrate Feeding During the Growing Period Induces Region-Specific Growth and Alters Growth Hormone Dynamics in Japanese Black Heifers.","authors":"Taketo Haginouchi, Tomiko Fumita, Daichi Nishino, Yutaka Suzuki, Shotaro Nishimura, Takafumi Gotoh","doi":"10.1111/asj.70213","DOIUrl":"10.1111/asj.70213","url":null,"abstract":"<p><p>This study investigated the effects of high-concentrate (HC) feeding during the growing stage on growth allocation and endocrine dynamics in Japanese Black heifers. Six heifers were assigned to a HC (3.0% body weight [BW]/day) or control (CON: 1.5% BW/day) group from 5 to 10 months of age. Feed intake, BW, muscle, bone, fat depot and visceral organ weights, histological traits of longissimus thoracis (LT), serum metabolites, and growth hormone (GH) dynamics were evaluated. At 10 months, the HC group showed greater BW and total muscle weight, with 18 muscles being heavier. The radius and ulna weights increased, but BW-adjusted ratios of several load-bearing bones tended to decrease. Subcutaneous fat deposition increased. LT adipocyte diameter and fat contents were greater, while muscle fiber type composition was unchanged. Liver weight and γ-glutamyl transferase activity increased. GH pulsatile secretion was reduced, whereas pituitary GH-positive cells, circulating IGF-1, and insulin concentrations remained unchanged. These findings indicate that HC feeding promotes overall growth but induces nonuniform development across tissues, reflecting a shift in growth allocation and endocrine regulation. Nutritional conditions during the growing stage may therefore play a critical role in shaping tissue development and subsequent productivity.</p>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"97 1","pages":"e70213"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148374336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of Eating Posture Detection Artificial Intelligence Models Trained on Different Supervised Datasets and Prototyping of Real-Time Feed Intake Estimation Tool for Broilers. 基于不同监督数据集训练的进食姿态检测人工智能模型评估及肉鸡采食量实时估计工具的原型设计
IF 1.6 3区 农林科学
Animal Science Journal Pub Date : 2026-01-01 DOI: 10.1111/asj.70162
Tong Meng, Hiroki Matsuyama, Shinsuke Konno, Kenichi Horiguchi
{"title":"Evaluation of Eating Posture Detection Artificial Intelligence Models Trained on Different Supervised Datasets and Prototyping of Real-Time Feed Intake Estimation Tool for Broilers.","authors":"Tong Meng, Hiroki Matsuyama, Shinsuke Konno, Kenichi Horiguchi","doi":"10.1111/asj.70162","DOIUrl":"10.1111/asj.70162","url":null,"abstract":"<p><p>This study investigated the impact of supervised dataset content on the accuracy of object detection Artificial Intelligence (AI) model for detecting eating postures (EP) in broilers. The findings confirmed that (1) more individuals in EP did not necessarily improve the AI model's detection accuracy, and using still images with different numbers of such individuals could enhance the AI model's ability to detect EP. (2) Including both EP and postures similar to eating postures (SEP) was found to improve detection accuracy; additionally, adding still images that contain only SEP also enhanced detection accuracy; moreover, accuracy was higher when EP and SEP were included in the same still image than in separate images. (3) Ensuring an equal proportion of still images containing different numbers of individuals in EP and in SEP was necessary. Furthermore, a tool to estimate broiler feed intake in real time was prototyped. Using this tool, eight feed intake estimations of 0.5 h were conducted for five broilers under group management, resulting in the mean absolute error (MAE) of 4.3 g and the mean absolute percentage error (MAPE) of 13.3%, indicating good estimation accuracy.</p>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"97 1","pages":"e70162"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147430393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of Approaches to Cattle Calving Prediction Based on Vaginal Temperature: Machine Learning-Versus Threshold-Based Models. 基于阴道温度的牛产犊预测方法评估:机器学习与基于阈值的模型。
IF 1.6 3区 农林科学
Animal Science Journal Pub Date : 2026-01-01 DOI: 10.1111/asj.70235
Masafumi Miwa, Kaiyu Kubota, Tsumugi Yoshiyama, Miki Sakatani
{"title":"Evaluation of Approaches to Cattle Calving Prediction Based on Vaginal Temperature: Machine Learning-Versus Threshold-Based Models.","authors":"Masafumi Miwa, Kaiyu Kubota, Tsumugi Yoshiyama, Miki Sakatani","doi":"10.1111/asj.70235","DOIUrl":"https://doi.org/10.1111/asj.70235","url":null,"abstract":"<p><p>Prediction of calving among cattle based on body temperature is used to prevent calving accidents. However, conventional threshold-based methods often miss or misdetect signs of calving. We quantitatively compared a machine learning-based model with conventional threshold-based methods for calving prediction using vaginal temperature data from wearable devices. Vaginal temperature was recorded at 5-min intervals from approximately 7 days before the expected calving date until parturition in beef (n = 77) and dairy cattle (n = 78). Twenty-six time-series features were extracted, and a machine learning-based calving prediction model was developed using gradient-boosted decision trees. The model predicting calving within 24 h achieved 84.4% and 94.9% sensitivity and 60.2% and 56.1% precision in beef and dairy cattle, respectively, compared with 70.1% and 76.9% sensitivity and 38.3% and 51.7% precision for a conventional threshold-based model using fixed threshold values derived from previous literature. Furthermore, the model predicting calving within 12 h achieved performance comparable to that of the 24-h model (81.8% and 84.6% sensitivity; 50.0% and 42.3% precision, respectively), suggesting the feasibility of customizable alert timing across multiple time windows. Further improvements, including larger datasets and refined feature engineering and model optimization, are needed to reduce missed and false detections.</p>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"97 1","pages":"e70235"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13494690/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148787360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Metabolomic Profiles Associated With Difference in Muscle Protein Degradation Between Fast- and Slow-Growing Chicks During the Neonatal Period. 新生期快、慢生长鸡肌肉蛋白降解差异的代谢组学分析
IF 1.6 3区 农林科学
Animal Science Journal Pub Date : 2026-01-01 DOI: 10.1111/asj.70169
Saki Shimamoto, Hanae Katayama, Daichi Ijiri, Shinobu Fujimura, Kazuki Nakashima, Shinya Ishihara, Shozo Tomonaga, Akira Ohtsuka, Miyu Kamimura
{"title":"Metabolomic Profiles Associated With Difference in Muscle Protein Degradation Between Fast- and Slow-Growing Chicks During the Neonatal Period.","authors":"Saki Shimamoto, Hanae Katayama, Daichi Ijiri, Shinobu Fujimura, Kazuki Nakashima, Shinya Ishihara, Shozo Tomonaga, Akira Ohtsuka, Miyu Kamimura","doi":"10.1111/asj.70169","DOIUrl":"10.1111/asj.70169","url":null,"abstract":"<p><p>We aimed to identify metabolites and metabolic pathways involved in differences in muscle protein degradation in broiler chicks during the neonatal period. To this end, we performed gas chromatography-mass spectrometry-based metabolomic analyses of plasma, pectoralis major muscle, and liver samples. Based on body weight gain from 1 to 5 days of age, 5-day-old broiler chicks (Ross308) were divided into three groups (slow-, intermediate-, and fast-growing groups). First, we confirmed that muscle protein degradation levels were closely associated with body weight gain. Untargeted metabolomic analysis revealed that 4, 14, and 22 metabolites in plasma, muscle, and liver, respectively, were correlated with the body weight gain of chicks from 1 to 5 days of age (Pearson's correlation, |r| ≥ 0.4). Most metabolic pathways identified in plasma and muscle were involved in either protein or amino acid metabolism, while those in liver were related to tricarboxylic acid cycle and carbohydrate metabolism. Interestingly, the expression of genes encoding enzymes related to branched-chain amino acid (BCAA) catabolism were higher in the slow-growing chicks than in the fast-growing ones. These results suggest that BCAA catabolism is important for muscle protein degradation levels and body growth of broiler chicks during the neonatal period.</p>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"97 1","pages":"e70169"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13037696/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147580275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Changes in Antimicrobial Component Concentrations in Goat Milk Following Intramammary Lipopolysaccharide Administration. 乳内脂多糖对羊奶抗菌成分浓度的影响。
IF 1.6 3区 农林科学
Animal Science Journal Pub Date : 2026-01-01 DOI: 10.1111/asj.70191
Jirapat Jaisue, Moeka Nishimura, Naoki Suzuki, Takahiro Nii, Naoki Isobe
{"title":"Changes in Antimicrobial Component Concentrations in Goat Milk Following Intramammary Lipopolysaccharide Administration.","authors":"Jirapat Jaisue, Moeka Nishimura, Naoki Suzuki, Takahiro Nii, Naoki Isobe","doi":"10.1111/asj.70191","DOIUrl":"10.1111/asj.70191","url":null,"abstract":"<p><p>Mastitis is a major cause of economic losses in dairy production; however, its prevention and treatment remain challenging. Under field conditions, antimicrobial drugs are frequently administered when somatic cell counts (SCCs) are elevated and signs of inflammation are observed, even in the absence of detectable pathogens in milk. As antimicrobial therapy may be unnecessary during the healing phase of inflammation, improved indicators of the mastitis stage are required. Therefore, this study aimed to determine whether changes in milk antimicrobial component (AMC) concentrations could serve as indicators of the inflammatory stage, thereby supporting judicious antimicrobial use. Lactating Tokara goats received an intramammary infusion of lipopolysaccharide (LPS) to induce experimental mastitis, with saline-infused quarters serving as controls. Milk samples were collected for up to 120 h post-infusion to measure SCC and the concentrations of lactoferrin (LF), goat β-defensin 1 (GBD1), cathelicidin-7, S100A8, and milk amyloid A (MAA). SCC peaked at 10 h, whereas GBD1 tended to peak at 4 h, indicating early inflammation. In contrast, LF, S100A8, and MAA, together with the increasing tendency of Cath-7 levels, peaked later (36-84 h) as SCC declined, suggesting an association with the recovery phase. These findings demonstrate that temporal patterns of milk AMCs can be used to stage mastitis progression.</p>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"97 1","pages":"e70191"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13184577/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147969970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A Technique for Nonmicrobial Freshness Evaluation of Broiler Breast Meat Using a Handheld Electrical Impedance Meter. 手持式电阻抗计评价肉鸡胸肉非微生物新鲜度技术
IF 1.6 3区 农林科学
Animal Science Journal Pub Date : 2026-01-01 DOI: 10.1111/asj.70241
Haruna Shinji, Chiaki Yamamoto, Masayoshi Otake, Shuichi Okabe, Makoto Otsuka
{"title":"A Technique for Nonmicrobial Freshness Evaluation of Broiler Breast Meat Using a Handheld Electrical Impedance Meter.","authors":"Haruna Shinji, Chiaki Yamamoto, Masayoshi Otake, Shuichi Okabe, Makoto Otsuka","doi":"10.1111/asj.70241","DOIUrl":"10.1111/asj.70241","url":null,"abstract":"<p><p>This study investigated the applicability of a handheld electrical impedance meter (EIM) for nondestructive evaluation of the freshness of broiler breast meat during refrigerated storage. Impedance ratios (2/100 kHz) were measured on Days 0, 1, 2, 3, 5, 7, and 10 of refrigerated storage and were compared with the K-values obtained through high-performance liquid chromatography (HPLC) analysis of adenosine triphosphate (ATP) degradation products. The impedance ratio decreased consistently over time, with SD decreasing from 0.36 on Day 0 to 0.07 on Day 10. A quadratic regression model showed that the impedance ratio decreased with the number of storage days (R<sup>2</sup> = 0.7561). The K-value increased over time and was also well described by a quadratic regression model (R<sup>2</sup> = 0.8395). The impedance ratio was strongly negatively correlated with the K-value (r = -0.730; n = 140; p < 0.001), supporting its utility for tracking freshness-related changes. Although the K-value demonstrated a slightly higher explanatory power, the impedance ratio showed sufficient accuracy for practical applications. These findings suggest that the EIM method offers a rapid, simple, and nondestructive alternative to conventional biochemical assays, with potential for implementation in poultry processing and retail environments for on-site freshness assessment.</p>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"97 1","pages":"e70241"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13547757/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148900084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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