Animal Science Journal最新文献_第9页

Can fecal characteristics be used to predict the digestibility of certain macro minerals in dry and lactating cows? 能否利用粪便特征预测干奶牛和泌乳奶牛对某些宏量矿物质的消化率？

IF 1.7 3区农林科学

Animal Science Journal Pub Date : 2025-01-13 DOI: 10.1111/asj.70017

Emre Yilmaz, Soner Uysal

{"title":"Can fecal characteristics be used to predict the digestibility of certain macro minerals in dry and lactating cows?","authors":"Emre Yilmaz, Soner Uysal","doi":"10.1111/asj.70017","DOIUrl":"10.1111/asj.70017","url":null,"abstract":"This study aims to evaluate the relationship between mineral digestibility and fecal characteristics and compare digestibility in dry and late-lactating cows. A total of 107 multiparous Holstein and Simmental cows were included, with 66 cows in late lactation and 41 cows in the dry period. The apparent digestibility of key macro minerals, dry matter content in feces, dirtiness scores, fecal characteristics, and serum macro mineral levels were determined. Cows consuming the same diet were arranged according to a completely randomized design. Results showed that, compared to the late lactation group, the dry period group had a significantly lower total dirtiness score, higher phosphorus digestibility, and elevated serum calcium levels (p < 0.05). Additionally, fecal consistency and fecal height were greater in the dry period group (p < 0.05). However, the increased phosphorus digestibility observed during the dry period was not significantly associated with fecal consistency (r = +0.225, p < 0.05) or the total contamination score (r = −0.339, p < 0.05). Consequently, this study reveals that understanding the differences in mineral digestibility between different physiological stages can enhance nutritional approaches for better dairy cow management.","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11726135/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142969423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of graded levels Citri Reticulatae Pericarpium (Chenpi) on growth performance, serum biochemical indices, meat quality, and caecal microbiota and metabolite in yellow-feathered broilers 分级水平柑桔皮对黄羽肉鸡生长性能、血清生化指标、肉品质的影响以及盲肠微生物群和代谢物的影响

IF 1.7 3区农林科学

Animal Science Journal Pub Date : 2025-01-10 DOI: 10.1111/asj.70025

Ying Ju, Liangliang Qi, Yongqiang Hu, Liling Huang, Liying Li, Yi Luo, Xiaoguang Shi, Xuzhou Liu, Mingguo Jiang

{"title":"Effects of graded levels Citri Reticulatae Pericarpium (Chenpi) on growth performance, serum biochemical indices, meat quality, and caecal microbiota and metabolite in yellow-feathered broilers","authors":"Ying Ju, Liangliang Qi, Yongqiang Hu, Liling Huang, Liying Li, Yi Luo, Xiaoguang Shi, Xuzhou Liu, Mingguo Jiang","doi":"10.1111/asj.70025","DOIUrl":"10.1111/asj.70025","url":null,"abstract":"The Citri Reticulatae Pericarpium (CRP), is the aged peel of Citrus fruit, which contains phenols, flavonoids, and polysaccharides. This study aims to investigate dietary CRP supplementation on the growth performance, serum biochemical indices, meat quality, intestinal morphology, microbiota, and metabolite of yellow-feathered broilers. A total of 240 yellow-feathered broilers (1.00 ± 0.22 kg, 9 weeks old) were randomly allotted into 4 treatments feeding a basal diet (control), the basal diet containing antibiotics (positive control), and the control diet containing 1.5% and 3% CRP, respectively. At the end of a 56-day trial, one broiler from each replicate was selected, and samples of ileal tissue and cecal digesta were collected to analyze intestinal morphology, microbial composition, and metabolites. The results revealed that 3% CRP decreased average daily feed intake (ADFI), serum total cholesterol (TC), and low-density lipoprotein (LDL). The CRP supplementation could increase serum superoxide dismutase (SOD) and meat pH. Broilers fed CRP had elevated antioxidant and liquid-regulated metabolites. Together, the results suggested that incorporating a moderate level of CRP has a minor impact on growth performance and could benefit health to some extent by enhancing antioxidants, regulating serum liquid, and changing microbial composition and metabolites.","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142942845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of dietary supplementation of myristic acid on jejunal mucosa-associated microbiota, mucosal immunity, and growth performance of nursery pigs 饲粮中添加肉豆蔻酸对育幼猪空肠黏膜相关微生物群、黏膜免疫和生长性能的影响

IF 1.7 3区农林科学

Animal Science Journal Pub Date : 2025-01-08 DOI: 10.1111/asj.70027

Hyunjun Choi, Gabriel Cipriano Rocha, Sung Woo Kim

{"title":"Effects of dietary supplementation of myristic acid on jejunal mucosa-associated microbiota, mucosal immunity, and growth performance of nursery pigs","authors":"Hyunjun Choi, Gabriel Cipriano Rocha, Sung Woo Kim","doi":"10.1111/asj.70027","DOIUrl":"10.1111/asj.70027","url":null,"abstract":"The objective of this study was to investigate the effects of myristic acid on jejunal mucosal microbiota, mucosal immunity, and growth performance of nursery pigs. Thirty-six pigs (6.6 ± 0.4 kg of body weight) were assigned to three treatments (n = 12) for 35 d in three phases: (NC) basal diet; (PC) NC + bacitracin; and (MA) NC + myristic acid compound. Pigs were euthanized to collect jejunal mucosa, jejunal tissues, and ileal digesta. The PC increased (p < 0.05) the relative abundance (RA) of Lactobacillus spp., and Bifidobacterium boum than the NC group. The MA increased (p < 0.05) RA of Bifidobacterium dentium and Megasphaera spp. than the NC group. The PC tended to decrease IL-8 (p = 0.053) and protein carbonyl (p = 0.075) whereas IgG (p = 0.051) and IL-8 (p = 0.090) in jejunal mucosa were decreased by the MA. The PC increased (p < 0.05) the villus height to crypt depth ratio than the NC group. Both bacitracin and myristic acid improved the intestinal health and growth performance of nursery pigs. Effects of bacitracin were rather immediate whereas the effects of myristic acid were obtained after a 3-week feeding.","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11707569/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142942844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impedance measurement for nondestructive venison pH estimation using touch-type electrodes 接触式电极无损测量鹿肉pH值。

IF 1.7 3区农林科学

Animal Science Journal Pub Date : 2025-01-07 DOI: 10.1111/asj.70026

Akari Igarashi, Takayuki Muramoto

{"title":"Impedance measurement for nondestructive venison pH estimation using touch-type electrodes","authors":"Akari Igarashi, Takayuki Muramoto","doi":"10.1111/asj.70026","DOIUrl":"10.1111/asj.70026","url":null,"abstract":"The pH of venison is affected by deer capture methods, which affects its water-holding capacity (WHC) during heating. Therefore, cooking or processing venison requires careful consideration of WHC at different pH levels. While this requires nondestructively measuring the pH of venison during distribution, there are no established methods to nondestructively distinguish between normal- and high-pH meats. We investigated the relationship between the pH of venison and impedance measured with touch-type electrodes. Significant negative correlations were found between pH before and after storage and drip loss (p < 0.05) and between impedance before storage using touch-type electrodes measured at 10 kHz and pH after 24 h of storage (p < 0.01). These results provided an estimating equation (y = −0.10x + 10.84) that can be used to nondestructively determine the pH of venison (y) after 24 h of storage by measuring its impedance (x) before storage using touch-type electrodes measured at 10 kHz (p < 0.01). Therefore, this study suggests the possibility of nondestructively estimating venison pH by measuring impedance using touch-type electrodes.","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11707497/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142942846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of volatile compound profile in Mongolian traditional fermented mare's milk, as Airag 蒙古传统发酵马奶中挥发物组分的研究。

IF 1.7 3区农林科学

Animal Science Journal Pub Date : 2025-01-05 DOI: 10.1111/asj.70024

Oyundelger Ganzorig, Batjargal Batdorj, Ishii Satomi

{"title":"Characterization of volatile compound profile in Mongolian traditional fermented mare's milk, as Airag","authors":"Oyundelger Ganzorig, Batjargal Batdorj, Ishii Satomi","doi":"10.1111/asj.70024","DOIUrl":"10.1111/asj.70024","url":null,"abstract":"We aimed to evaluate the volatile compounds profile in traditional airag samples collected from two regions, including Bulgan and Uvurkhangai provinces, whereas famous airag-making areas in Mongolia. The volatile compounds of airag were investigated by the GC–MS method. A total of 95 kinds of volatile compounds were detected, and these were classified into 6 different classes: 14 acids, 14 alcohols, 16 aldehydes, 19 esters, 9 ketones, and 23 aliphatic hydrocarbons. Among them, acetic acid, 2-methyl propionic acid, isoamyl lactic acid, 2-methyl butanoic acid, hexanoic acid, heptanoic acid, octanoic acid, nonanoic acid, n-decanoic acid, 1-propanol, 3-methyl 1-butanol, phenyl ethyl alcohol, acetaldehyde, acetoin, ethyl acetate, ethyl hexanoate, ethyl caprylate, ethyl decanoate, ethyl 9-decanoate, and ethyl laurate had a high concentration in airag samples. It may explain that acids, alcohols, and esters mainly influence the taste and aroma of airag, and aldehydes change depending on their fermentation time. We also found that Bulgan provinces` samples contained 20 types of aliphatic hydrocarbons, which may relate to the vegetation and fermentation process. The formation of sensory characteristics in airag is a complex and unclear process because it affects many factors. Our findings provide essential guidance on the composition of volatile compounds in Mongol airag.","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142930721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Discrimination thresholds for glutamic acid and chicken oil in chicken broth assessed by an untrained panel at a research institute 鸡汤中谷氨酸和鸡油的鉴别阈值由一个未经培训的研究机构小组评估。

IF 1.7 3区农林科学

Animal Science Journal Pub Date : 2025-01-05 DOI: 10.1111/asj.70022

Genya Watanabe, Shota Ishida, Karin Akada, Masaya Komatsu, Hiroki Sakurai, Saki Shinoda, Michiyo Motoyama, Ikuyo Nakajima, Atsushi Asano, Yuji Miyaguchi, Keisuke Sasaki

{"title":"Discrimination thresholds for glutamic acid and chicken oil in chicken broth assessed by an untrained panel at a research institute","authors":"Genya Watanabe, Shota Ishida, Karin Akada, Masaya Komatsu, Hiroki Sakurai, Saki Shinoda, Michiyo Motoyama, Ikuyo Nakajima, Atsushi Asano, Yuji Miyaguchi, Keisuke Sasaki","doi":"10.1111/asj.70022","DOIUrl":"10.1111/asj.70022","url":null,"abstract":"Glutamic acid (Glu) and fat are recognized as primary flavor components in meat. This study aimed to determine the discrimination threshold of an untrained panel for Glu and fat in meat. Thirty-two untrained panelists were recruited among research institute employees. First, triangle tests were performed using chicken broth supplemented with Glu at concentrations of 10, 50, 110, and 200 μg/ml and broth without Glu supplementation (control). The Glu supplementation test did not distinguish between Glu-supplemented broth and the control. Thus, the discrimination threshold for Glu in chicken broth was determined to be >200 μg/ml. Second, triangle tests were performed using chicken oil-emulsified broth at concentrations of 43, 129, 387, and 1160 μg/ml and broth without chicken oil supplementation. The probability of choosing the correct sample in the triangle test for each chicken oil concentration was calculated. The probit regression analysis estimated the discrimination threshold for chicken oil in chicken broth to be 341 μg/ml among untrained panelists. Although the number of untrained panelists recruited in this study was limited, our results provided useful findings for evaluating and improving meat quality utilizing Glu and fat content.","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142930034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Control of Recessive Genetic Disorders in Japanese Black Cattle Using DNA-Based Genetic Diagnostic Tests. 利用基于dna的遗传诊断试验控制日本黑牛的隐性遗传疾病。

IF 1.2 3区农林科学

Animal Science Journal Pub Date : 2025-01-01 DOI: 10.1111/asj.70121

Atsushi Ogino, Yoshiyuki Miyazaki, Yuri Suzuki, Gaku Tahara, Shinji Sasaki, Kazuhito Kurogi, Masanobu Nurimoto

{"title":"Control of Recessive Genetic Disorders in Japanese Black Cattle Using DNA-Based Genetic Diagnostic Tests.","authors":"Atsushi Ogino, Yoshiyuki Miyazaki, Yuri Suzuki, Gaku Tahara, Shinji Sasaki, Kazuhito Kurogi, Masanobu Nurimoto","doi":"10.1111/asj.70121","DOIUrl":"https://doi.org/10.1111/asj.70121","url":null,"abstract":"The effective control of genetic disorders is essential for animal welfare and sustainable production. Since 1996, 10 causative mutations in autosomal recessive genetic disorders have been identified in Japanese Black cattle, leading to the development of DNA-based genetic diagnostic tests. The government has published profile sheets detailing genetic disorders and designated seven of these disorders as having a significant economic impact, prompting a response policy using DNA-based genetic diagnostic tests to reduce their occurrence. However, the effects of these tests on the frequency of risk alleles have not yet been evaluated. In this study, we analyzed the risk allele frequency trends over two decades using a large-scale dataset from central slaughterhouses. We found that following the publication of the profile sheet, the frequencies of the risk alleles rapidly declined, reaching very low levels. For such a rapid decline to occur within three generations, a significant proportion of asymptomatic heterozygous carriers would need to have been eliminated from the breeding system. This level of removal is considered unattainable through natural selection alone, indicating the need for DNA-based genetic diagnostic tests. These results showed that DNA-based genetic diagnostic tests were effective in suppressing the frequency of risk alleles in the population.","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":"e70121"},"PeriodicalIF":1.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145243608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective Effects of α-Linolenic Acid on Liver Inflammation and Oxidative Stress Induced by Lipopolysaccharide in Broilers. α-亚麻酸对脂多糖诱导肉鸡肝脏炎症和氧化应激的保护作用

IF 1.2 3区农林科学

Animal Science Journal Pub Date : 2025-01-01 DOI: 10.1111/asj.70096

Xinyu Cheng, Xuelin Zhang, Jialei Ni, Houkang Ding, Weiming Ma, Dong Wang

{"title":"Protective Effects of α-Linolenic Acid on Liver Inflammation and Oxidative Stress Induced by Lipopolysaccharide in Broilers.","authors":"Xinyu Cheng, Xuelin Zhang, Jialei Ni, Houkang Ding, Weiming Ma, Dong Wang","doi":"10.1111/asj.70096","DOIUrl":"https://doi.org/10.1111/asj.70096","url":null,"abstract":"This study evaluated the protective effects of dietary α-linolenic acid (ALA) on liver inflammation and oxidative stress induced by lipopolysaccharide (LPS) in broilers. A total of 256 AA broilers were randomly allocated to 4 groups (Con, LPS, LPS + ALA400, and LPS + ALA600) with 8 replicates. On d 20, the Con group broilers were intraperitoneally injected with sterile saline, and the other groups were injected with LPS (0.5 mg/kg of body weight). The trial lasted for 21 days. Results indicated that there were no significant differences in growth performance across the groups. ALA supplementation at 600 mg/kg significantly improved the levels of low-density lipoprotein cholesterol, serum and liver catalase, total antioxidant capacity, and superoxide dismutase while decreasing the levels of aspartate aminotransferase, alkaline phosphatase and malondialdehyde as compared with the LPS group broilers. Moreover, dietary supplementation with 600 mg/kg ALA markedly attenuated LPS-induced inflammation and oxidative stress by downregulating the expression of pro-inflammatory cytokines (IL-1β, TNF-α, and IFN-γ) and the TLR4/NF-κB pathway, while upregulating the expression of antioxidant enzymes (CAT, GSH-Px, SOD1) and the Nrf2/HO-1 pathway. In conclusion, ALA supplementation mitigates LPS-induced liver inflammation and oxidative stress in broilers by enhancing liver antioxidant capacity and modulating TLR4/NF-κB and Nrf2/HO-1 signaling pathways.","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":"e70096"},"PeriodicalIF":1.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144798016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Possible Involvement of CSPG4 in Promoting Endothelial Cell Migration and Contributing to Angiogenesis during Skeletal Muscle Regeneration and Development in the Rat. CSPG4在大鼠骨骼肌再生和发育过程中促进内皮细胞迁移和血管生成的可能参与。

IF 1.2 3区农林科学

Animal Science Journal Pub Date : 2025-01-01 DOI: 10.1111/asj.70097

Riku Yamaguchi, Masanari Ikeda, Shiho Takeuchi, Katsuyuki Nakamura, Akbota Kurmangaliyeva, Naomi Teramoto, Hidetoshi Sugihara, Chihiro Kikawa, Takashi Matsuwaki, Masugi Nishihara, Keitaro Yamanouchi

{"title":"Possible Involvement of CSPG4 in Promoting Endothelial Cell Migration and Contributing to Angiogenesis during Skeletal Muscle Regeneration and Development in the Rat.","authors":"Riku Yamaguchi, Masanari Ikeda, Shiho Takeuchi, Katsuyuki Nakamura, Akbota Kurmangaliyeva, Naomi Teramoto, Hidetoshi Sugihara, Chihiro Kikawa, Takashi Matsuwaki, Masugi Nishihara, Keitaro Yamanouchi","doi":"10.1111/asj.70097","DOIUrl":"10.1111/asj.70097","url":null,"abstract":"Skeletal muscle regeneration is a complex process that requires coordinated interactions between myogenic and vascular cells. Chondroitin sulfate proteoglycan 4 (CSPG4), a cell surface proteoglycan, had been shown to be expressed around immature myofibers in patients with Duchenne muscular dystrophy, suggesting its role in muscle regeneration. In the present study, we found that CSPG4 is transiently expressed by regenerating myofibers upon muscle injury in the rat. We generated the CSPG4 knockout (KO) rats to investigate its role in muscle regeneration. In CSPG4 KO rats, skeletal muscle development was perturbed with impaired angiogenesis. When muscle regeneration was induced, CSPG4 KO rats showed a reduced number of CD31-positive cells at the regenerating site. These results suggest the role of CSPG4 in angiogenesis during muscle regeneration and development. In vitro, coculture of CSPG4-expressing mesenchymal progenitor cells (MPC) promoted vascular endothelial cell migration, whereas the KO of CSPG4 in MPC abrogated the upregulation of endothelial cell migration. Our findings identify CSPG4 as a key regulator of vascular endothelial cell recruitment during muscle vascular remodeling and highlight its potential as a therapeutic target for Duchenne muscular dystrophy, which is characterized by impaired angiogenesis.","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":"e70097"},"PeriodicalIF":1.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12328044/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144793279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient Isolation and Characterization Methods for Chicken Serum Extracellular Vesicles. 鸡血清细胞外囊泡的高效分离与表征方法

IF 1.2 3区农林科学

Animal Science Journal Pub Date : 2025-01-01 DOI: 10.1111/asj.70092

Hatsune Mukohda, Muhammet Mustafa Binici, Katsuya Miyake, Yoshitaka Sano, Takahisa Yamada, Takumi Terada, Toshie Sugiyama

{"title":"Efficient Isolation and Characterization Methods for Chicken Serum Extracellular Vesicles.","authors":"Hatsune Mukohda, Muhammet Mustafa Binici, Katsuya Miyake, Yoshitaka Sano, Takahisa Yamada, Takumi Terada, Toshie Sugiyama","doi":"10.1111/asj.70092","DOIUrl":"https://doi.org/10.1111/asj.70092","url":null,"abstract":"Extracellular vesicles (EVs) are small cell membrane-derived vesicles that are known as mediators of cell-to-cell communication. Efficient methods for chicken serum EV isolation and identification have not been established to satisfy the international guidelines of the Minimal Information for Studies of Extracellular Vesicles (MISEV). Therefore, the present study isolated EVs from chicken serum using four conventional methods-ultracentrifugation (UC), two polymer-based precipitations (PP), and filtration (FL)-and characterized them by western blotting (WB) using polyclonal antibodies (anti-CD9, anti-PDCD6IP, anti-TSG101, and anti-calnexin), nanoparticle tracking analyses, and transmission electron microscopy of MISEV guidelines. As a result, all isolated samples represented specific EV marker proteins, small particles sized 30-150 nm, and spherical morphology with a double membrane structure. In particular, FL-isolated EVs showed the most distinct expression of the marker proteins by WB analysis and the highest expression of miR-451, which is a stable microRNA of EVs, by quantitative PCR, suggesting that FL could isolate highly purified EVs. In conclusion, we established an optimal characterization method for chicken EVs and estimated the efficiency of EV isolation methods from chicken serum.","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":"e70092"},"PeriodicalIF":1.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144798014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0