Animal Science Journal最新文献

{"title":"Ursodeoxycholic Acid Alleviates Palmitic Acid–Induced Apoptosis in Bovine Mammary Epithelial Cells","authors":"Md. Rezwanul Habib,&nbsp;Yukako Tokutake,&nbsp;Shinichi Yonekura","doi":"10.1111/asj.70038","DOIUrl":"https://doi.org/10.1111/asj.70038","url":null,"abstract":"<p>This study investigated the protective effects of ursodeoxycholic acid (UDCA) against PA-induced apoptosis in the MAC-T bovine mammary epithelial cell (bMEC) line by assessing the level of cell viability, oxidative stress indicators, and expression of endoplasmic reticulum (ER) stress markers. MAC-T cells were pretreated with UDCA at 25, 50, and 100 μM before exposure to PA at 300 μM. UDCA was noncytotoxic at these concentrations and significantly improved cell viability, which was reduced by PA. UDCA pretreatment notably decreased PA-induced expression of GRP78, XBP1s, ATF4, and CHOP mRNA, indicating reduced ER stress. Moreover, UDCA substantially lowered PA-induced reactive oxygen species production and dichlorofluorescein fluorescence intensities. Although PA treatment elevated GSSG levels and disrupted redox balance by decreasing both the total GSH/GSSG and reduced GSH/GSSG ratios, UDCA effectively counteracted these effects. Specifically, UDCA reduced PA-induced GSSG levels and restored redox balance by increasing both total and reduced GSH/GSSG ratios. These findings suggest that UDCA mitigates PA-induced apoptosis in MAC-T cells by enhancing redox homeostasis. Therefore, incorporating UDCA into dairy cow feed could help reduce PA-induced cellular stress and improve milk production during periods of negative energy balance.</p>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/asj.70038","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143380378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Curcumin Suppresses ROS Production and Increases Mitochondrial Activity in Cumulus Cells and Oocytes of COCs Derived From Non-Vascularized Follicles in Pigs","authors":"Tomoya Nakanishi,&nbsp;Shingo Tonai,&nbsp;Haruto Ichikawa,&nbsp;Shota Mori,&nbsp;Shinji Ishihara,&nbsp;Yongjin Chang,&nbsp;Yasuhisa Yamashita","doi":"10.1111/asj.70032","DOIUrl":"https://doi.org/10.1111/asj.70032","url":null,"abstract":"<div>\u0000 \u0000 <p>In vitro maturation (IVM) produces offspring from domestic animals; however, the blastocyst rate after IVM was low. We previously reported that the developmental competence of oocytes derived from follicles with blood vessels absent on the surface (non-vascularized follicles: NVF) is quite low compared to those derived from follicles with blood vessels present on the surface (vascularized follicles: VF). Thus, it is important to develop technique to improve the quality of NVF-derived oocyte by IVM. Since it has been reported that reactive oxygen species (ROS) reduces oocyte quality, in this study, we investigated whether curcumin that is known as antioxidant could improve oocyte quality derived from NVF. As results, cultivation of NVF Cumulus-oocyte complexes (COCs) with curcumin significantly improved cumulus expansion and oocyte meiotic maturation of NVF COCs compared to those of NVF COCs without curcumin. Cultivation with curcumin of NVF COCs significantly improved the proliferative activity of cumulus cells. Furthermore, the cultivation significantly reduced ROS activity and increased mitochondrial activity. Hence, it was revealed that the addition of curcumin to the maturation medium increased mitochondrial activity and reduced ROS levels in NVF-derived cumulus cells and oocytes, thereby improving the maturation of oocytes within COCs.</p>\u0000 </div>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143112144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary Protein Reduction in Lean Pigs From Pietrain Paternal Line Under Commercial Setting: An Interplay Between Nitrogen Efficiency and Meat Attributes","authors":"Javier Álvarez-Rodríguez,&nbsp;Alba Vigo-Morancho,&nbsp;Francisco Javier Miana-Mena,&nbsp;Leticia Pérez-Ciria,&nbsp;Maria Angeles Latorre","doi":"10.1111/asj.70033","DOIUrl":"10.1111/asj.70033","url":null,"abstract":"<div>\u0000 \u0000 <p>A trial was conducted to research the impact of reducing dietary crude protein (CP) levels from 14.8% to 12.6% in the finishing diet (90–120 kg of body weight) of lean pigs, with an essential AA supply (8.8 g Lys, 2.1 g Met, 6.7 g Thr, 5.5 g Ile, and 6.7 g Val, per kilogram of feed) in intact males and females under commercial setting. The productive performances and nitrogen (N) balance, blood markers related to protein metabolism, apparent digestibility of nutrients, carcass, and meat quality were evaluated. This dietary CP restriction did not impair the growth performances, decreased the N excretion, and did not affect the apparent digestibility of most nutrients, except phosphorus digestibility, which was reduced in the low-CP diet. The responses to this dietary CP restriction were independent of sex, except the serum urea concentration and total proteins, which were only steadily reduced in intact males fed the low-CP diet. The low-CP diet reduced the nutritional pork quality by increasing the moisture content, although the technological characteristics of meat were not affected. Further studies are required to improve the sex-tailored feeding strategies for high-conformed lean pigs in an environmentally friendly context.</p>\u0000 </div>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143078459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acknowledgment to Reviewers","authors":"","doi":"10.1111/asj.70035","DOIUrl":"https://doi.org/10.1111/asj.70035","url":null,"abstract":"","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143121269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heat stress induces oxidative stress and activates the KEAP1-NFE2L2-ARE pathway in reproduction-related cells","authors":"Hanako Bai,&nbsp;Manabu Kawahara,&nbsp;Kazuya Kusama,&nbsp;Toshihiro Sakurai,&nbsp;Christiane Pfarrer,&nbsp;Masashi Takahashi","doi":"10.1111/asj.70023","DOIUrl":"10.1111/asj.70023","url":null,"abstract":"<p>Heat stress negatively affects the reproductive function of in animals and humans. Although a relationship between heat and oxidative stress has been suggested, the underlying mechanism has not been sufficiently examined in reproduction-related cells. Therefore, we aimed to investigate whether heat stress induces oxidative stress using a variety of reproduction-related cells including bovine placental and cumulus–granulosa cells, human cell lines derived from cervical and endometrial cancers, and fibroblasts derived from endometrium. Quantitative polymerase chain reaction analysis showed that the expression levels of representative heat and oxidative stress–related genes were significantly increased in cells cultured at high temperatures compared with those in cells cultured at basal temperatures. Moreover, luciferase reporter assays showed that the reporter activity of the heat shock element and antioxidant responsive element (ARE) was increased in cells cultured at high temperatures compared with that in cells cultured at basal temperatures. Furthermore, the stability of nuclear factor erythroid 2 like 2 (NFE2L2), a master regulator of the cellular stress response, increased under high temperatures. Point mutations in Kelch-like ECH-associated protein 1 (KEAP1) cysteine residues reduced the luciferase activity. Our results suggest that heat stress induces oxidative stress and that the KEAP1-NFE2L2-ARE pathway may play a protective role in reproduction-related cells against heat stress.</p>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143045141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physicochemical Properties and Contents of Amino Acids, Fatty Acids, and Phytanic Acid in Goat Meat Produced by Grazing System in an Abandoned Rice Field","authors":"Nthanda Chimwemwe Amini,&nbsp;Taketo Obitsu,&nbsp;Toshihisa Sugino,&nbsp;Shogo Shinde","doi":"10.1111/asj.70028","DOIUrl":"10.1111/asj.70028","url":null,"abstract":"<div>\u0000 \u0000 <p>This study evaluated carcass weight and physicochemical properties of loin and leg meat parts of Saanen castrated goats (<i>n</i> = 4) reared by grazing for 5 months in an abandoned rice field with concentrate supplements, comparing with meat of goats (<i>n</i> = 4) reared by confined feeding with grass silage and concentrate. Carcass and meat part weights of goats in the grazing group were lower (<i>p</i> &lt; 0.01) than confined group. Shear force and cooking loss of meat showed no differences between the feeding groups. For meat color, L* value of loin meat tended to be lower (<i>p</i> &lt; 0.15) for the grazing group compared with the confined group. Moisture content tended to be higher, but fat content tended to be lower (<i>p</i> &lt; 0.15) in loin meat for the grazing group. Carnosine and alanine contents in loin meat were higher (<i>p</i> &lt; 0.05) for the grazing group. Polyunsaturated fatty acid composition in meat was higher (<i>p</i> &lt; 0.05), whereas monounsaturated fatty acid composition was lower (<i>p</i> &lt; 0.05) for the grazing group. Phytanic acid content in both meat parts showed higher value (<i>p</i> &lt; 0.05) for the grazing group. These results indicate that goat meat produced by grazing system in an abandoned rice field contains beneficial compounds for human health.</p>\u0000 </div>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143045200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial Component Concentrations in the Milk of Peripartum Goats","authors":"Jin-Kun Sun,&nbsp;Zi-Long Liang,&nbsp;Takahiro Nii,&nbsp;Naoki Suzuki,&nbsp;Naoki Isobe","doi":"10.1111/asj.70029","DOIUrl":"10.1111/asj.70029","url":null,"abstract":"<p>The aim of this study was to investigate changes in milk conditions and antimicrobial components in goats during 8 weeks prepartum and 1 week postpartum. Milk was collected weekly from seven prepartum goats 8 weeks before the due day, immediately, and 1 week after parturition. Milk color scores and the concentration of antimicrobial components (cathelicidin-7 and S100A8) were significantly increased prepartum. There was a significant decrease in milk viscosity, color scores, somatic cell count, and antimicrobial components (lactoferrin and cathelicidin-2, 7) after kidding. However, S100A7 levels did not change throughout the prepartum or postpartum periods. These results indicate that concentrations of cathelicidin-7 and S100A8 differ between prepartum and postpartum periods. Therefore, these microbial components may play different roles in mastitis prevention. The findings of our study provide important information for regulating antimicrobial components in ruminants to prevent bacterial invasion during the peripartum period.</p>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11754004/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143021718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationships Between Genetic Parameters in the Component Traits of a Ratio Trait and the Distribution and Heritability of Such Ratio Trait","authors":"Masahiro Satoh","doi":"10.1111/asj.70031","DOIUrl":"10.1111/asj.70031","url":null,"abstract":"<p>The relationships between the distribution and heritability of a ratio trait and the genetic parameters of its component traits were clarified using a Monte Carlo computer simulation. The component traits of a ratio trait were assumed to be normally distributed. The genetic parameters of the ratio trait were estimated using parent–offspring regression. As the genetic and environmental correlations (<span></span><math>\u0000 <semantics>\u0000 <mrow>\u0000 <msub>\u0000 <mi>r</mi>\u0000 <mi>G</mi>\u0000 </msub>\u0000 </mrow>\u0000 <annotation>$$ {mathrm{r}}_{mathrm{G}} $$</annotation>\u0000 </semantics></math> and <span></span><math>\u0000 <semantics>\u0000 <mrow>\u0000 <msub>\u0000 <mi>r</mi>\u0000 <mi>E</mi>\u0000 </msub>\u0000 </mrow>\u0000 <annotation>$$ {mathrm{r}}_{mathrm{E}} $$</annotation>\u0000 </semantics></math>) of the component traits increased, the variance, skewness, and kurtosis of the ratio trait decreased. The amount of decrease had a greater effect on <span></span><math>\u0000 <semantics>\u0000 <mrow>\u0000 <msub>\u0000 <mi>r</mi>\u0000 <mi>E</mi>\u0000 </msub>\u0000 </mrow>\u0000 <annotation>$$ {mathrm{r}}_{mathrm{E}} $$</annotation>\u0000 </semantics></math> than on <span></span><math>\u0000 <semantics>\u0000 <mrow>\u0000 <msub>\u0000 <mi>r</mi>\u0000 <mi>G</mi>\u0000 </msub>\u0000 </mrow>\u0000 <annotation>$$ {mathrm{r}}_{mathrm{G}} $$</annotation>\u0000 </semantics></math>. The estimated heritability of the ratio trait decreased as <span></span><math>\u0000 <semantics>\u0000 <mrow>\u0000 <msub>\u0000 <mi>r</mi>\u0000 <mi>G</mi>\u0000 </msub>\u0000 </mrow>\u0000 <annotation>$$ {mathrm{r}}_{mathrm{G}} $$</annotation>\u0000 </semantics></math> increased and as <span></span><math>\u0000 <semantics>\u0000 <mrow>\u0000 <msub>\u0000 <mi>r</mi>\u0000 <mi>E</mi>\u0000 </msub>\u0000 </mrow>\u0000 <annotation>$$ {mathrm{r}}_{mathrm{E}} $$</annotation>\u0000 </semantics></math> decreased. The estimated heritability of a ratio trait can be higher than the heritability of any of its component traits.</p>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11754005/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143021729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Palmitic Acid in the Diet of Dairy Goats and Its Effect on Physicochemical Characteristics and Fatty Acid Profile of Goat Kid Meat","authors":"Yuridia Bautista-Martínez,&nbsp;Jorge Alonso Maldonado-Jáquez,&nbsp;José Felipe Orzuna-Orzuna,&nbsp;Pablo Arenas-Báez,&nbsp;Lorenzo Danilo Granados-Rivera","doi":"10.1111/asj.70030","DOIUrl":"10.1111/asj.70030","url":null,"abstract":"<div>\u0000 \u0000 <p>The objective of this study was to evaluate the impact of incorporating palmitic acid into the diet of dairy goats on the physicochemical characteristics, nutritional value, and fatty acid profile of goat kid meat. For this, 28 Creole local kids were used, divided into the following treatments: grazing, kids fed milk from grazing goats; base diet, kids fed milk from goats in confinement with a total mixed ration; base diet + 3PA, kids fed milk from goats in confinement that consumed the base diet plus 3% palmitic acid; and base diet + 6PA, kids fed milk from goats in confinement fed with the base diet plus 6% palmitic acid. The feeding period was conducted over a 30-day period. Live weight, average daily gain, carcass weight, and carcass yield were higher (<i>p</i> ≤ 0.01) in kids from the base diet + 6PA treatment than in the other treatments. The proportion of stearic, elaidic, linoleic, CLA, oleic, and docosahexaenoic fatty acids was higher (<i>p</i> ≤ 0.05) in meat from kids fed with grass-fed milk. In contrast, kids from the base diet + 3PA and base diet + 6PA treatments had a higher (<i>p</i> ≤ 0.05) proportion of palmitic and palmitoleic acids. The addition of palmitic acid to goat feed improves growth rate and carcass yield and modifies the fatty acid profile in kid meat.</p>\u0000 </div>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143021725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive omics analysis of the fecal microbiome and serum metabolome in Dezhou donkey foals at the end of weaning and after weaning","authors":"Qiwen Yang,&nbsp;Zhaofei Wang,&nbsp;Jiangtian Su,&nbsp;Ge Yang,&nbsp;Haibing Liu,&nbsp;Bing Liu,&nbsp;Jie Cheng,&nbsp;Boying Dong,&nbsp;Halima Jafari,&nbsp;Hua Wang,&nbsp;Youxin Zhang,&nbsp;Chuzhao Lei,&nbsp;Ruihua Dang,&nbsp;Jie Yu","doi":"10.1111/asj.70021","DOIUrl":"10.1111/asj.70021","url":null,"abstract":"<p>Weaning is essential for foal growth and development. We determined the intestinal flora structure of donkey foals at the end of weaning (PreW_4d) and three stages after weaning (PostW_4d, PostW_8d, and PostW_15d) to explore the effects of weaning on intestinal development of donkey foals. The results showed that the main microbial flora in the gut of the donkey foal were Firmicutes and Bacteroides, and the proportion of Firmicutes gradually increased with weaning, which was an important reflection of the donkey foal's adaptability to the transition from lactose liquid feed to plant fiber solid feed. We also identified important microorganisms that maintain intestinal stability and boost immune, such as oscillospiraceae, Firmicutes, and lachnospiraceae. The metabolome showed that serum metabolites were mainly enriched in arachidonic acid metabolism and the tricarboxylic acid cycle (TCA cycle), which can influence energy metabolism, growth, and immunity in weaned donkey foals. We also found that the metabolite resveratrol was positively correlated with <i>g_NK4A214_group</i> and lactobacillus, which may have important implications for the prevention of diseases such as colon-inflammation in donkey foals. In summary, we provide a theoretical basis for studying the mechanism of intestinal microbiome and serum metabolite changes in weaning and postweaning donkey foals.</p>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142998807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}