Animal Science Journal最新文献_第8页

The Potential Role of Two Red Macroalgae (Asparagopsis taxiformis and A. armata) That Promote Anti-Methanogenic Activities in Laboratory Conditions. 两种红藻（taxxiformasparagopsis和a.a armata）在实验室条件下促进抗产甲烷活性的潜在作用。

IF 1.6 3区农林科学

Animal Science Journal Pub Date : 2026-01-01 DOI: 10.1111/asj.70225

Byeng R Min, Giuseppa Genovese, Damiano Spagnuolo, Mariline Hilaire, Hossam Ismael, Santosh Chaudhary, Dipti W Pitta, Nagaraju Indugu

{"title":"The Potential Role of Two Red Macroalgae (Asparagopsis taxiformis and A. armata) That Promote Anti-Methanogenic Activities in Laboratory Conditions.","authors":"Byeng R Min, Giuseppa Genovese, Damiano Spagnuolo, Mariline Hilaire, Hossam Ismael, Santosh Chaudhary, Dipti W Pitta, Nagaraju Indugu","doi":"10.1111/asj.70225","DOIUrl":"10.1111/asj.70225","url":null,"abstract":"The objectives of this study were to evaluate the effects of two red macroalgae species (RMS; Asparagopsis taxiformis and A. armata) on rumen fermentation profiles, greenhouse gas (GHG) emissions, microbiome changes, and anti-methanogenic activities. The two RMSs were included at dietary levels of 0%, 2%, and 4% (as-fed basis) in an in vitro experiment with triplicate incubations (n = 3). Gases were collected using an ANKOM Gas Production system and analyzed for methane (CH4) and nitrous oxide (N2O) via gas chromatography. The RMS supplementation increased total gas, lactate, butyrate, valerate, hexanoate, heptanoate, 4-ethylphenol production, and AGR (non-glucogenic [acetate + butyrate]/glucogenic [propionate]) ratio (p < 0.01), while reducing production of CH4 (mg/g DM), acetate, propionate, iso-butyrate, phenylpropionate, phenylacetate, and acetate/propionate (A/P) ratios (p < 0.01), and in vitro dry matter digestibility (IVDMD; % DM) (p < 0.01) as RMS supplementation increased. With both algal species present, there were decreases in Actinobacteria, Firmicutes (p < 0.001), Firmicutes/Bacteroidetes ratio (F/B), and Methanobrevibacter sp. (p < 0.01), but increases in Spirochetes, Proteobacteria (methanotrophs; p < 0.001), Candidatus methanomethylophilus alvus (CMC), and non-methanogenic archaea Thermoplasma sp. (p < 0.001) at 2% and 4% DM. Therefore, it may be possible to suppress methanogenesis both directly and indirectly by adding RMS.","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"97 1","pages":"e70225"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13429947/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148663393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

5-Aminolevulinic Acid Improves Immune Response in Dairy Cows In Vivo and In Vitro. 5-氨基乙酰丙酸提高奶牛体内和体外免疫应答

IF 1.6 3区农林科学

Animal Science Journal Pub Date : 2026-01-01 DOI: 10.1111/asj.70222

Amin Omar Hendawy, Satoshi Sugimura, Shin Taniguchi, Kan Sato

{"title":"5-Aminolevulinic Acid Improves Immune Response in Dairy Cows In Vivo and In Vitro.","authors":"Amin Omar Hendawy, Satoshi Sugimura, Shin Taniguchi, Kan Sato","doi":"10.1111/asj.70222","DOIUrl":"10.1111/asj.70222","url":null,"abstract":"5-Aminolevulinic acid (5-ALA) is a naturally occurring amino acid, which plays an important role in the biosynthesis of heme. We investigated its immunomodulatory effect as a potential bioactive feed additive by evaluating the expression of immune-related genes and oxidative status in dairy cows. Using a crossover design, six lactating Holstein cows randomized to control and treatment groups received a total mixed ration (TMR) or TMR plus 10 mg 5-ALA/kg of dry matter for 2 weeks. In vitro, peripheral blood mononuclear cells (PBMC) obtained from untreated dairy cows were incubated with 5-ALA in the presence of Escherichia coli lipopolysaccharide (LPS). Tumor necrosis factor-α (TNF-α) mRNA expression significantly decreased in PBMC of dairy cows fed 5-ALA, and interleukin-1β (IL-1β) tended to decrease. 5-ALA had no effect on the cluster of differentiation 3, interferon-γ, and Toll-like receptors in PBMC and 1,1-diphenyl-2-picrylhydrazyl free radical scavenging activity, relative superoxide dismutase activity, and thiobarbituric acid reactive substances in the blood, milk, and ruminal fluid of dairy cows. In vitro, TNF-α expression decreased in PBMC treated with 5-ALA (10 μg/mL) in the presence of LPS compared with LPS alone. Thus, 5-ALA may optimize the inflammatory response by controlling the expression of TNF-α in PBMC and eventually improving immunity in dairy cows.","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"97 1","pages":"e70222"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13422138/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148618149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Maternal Undernutrition During Late Pregnancy Impairs Fetal Renal Development and Induces Apoptotic Pathway Activation in the Sheep Model. 母羊妊娠后期营养不良影响胎儿肾脏发育并诱导凋亡通路激活

IF 1.6 3区农林科学

Animal Science Journal Pub Date : 2026-01-01 DOI: 10.1111/asj.70173

Yina Qiao, Chi Ma, Yang Zi, Suting Shen, Mingyue Li, Yingchun Liu, Feng Gao

{"title":"Maternal Undernutrition During Late Pregnancy Impairs Fetal Renal Development and Induces Apoptotic Pathway Activation in the Sheep Model.","authors":"Yina Qiao, Chi Ma, Yang Zi, Suting Shen, Mingyue Li, Yingchun Liu, Feng Gao","doi":"10.1111/asj.70173","DOIUrl":"10.1111/asj.70173","url":null,"abstract":"This study investigated the effects of maternal nutritional restriction during late pregnancy on fetal kidney development in sheep. Eighteen Mongolian ewes with singleton fetuses were allocated to three groups at 90 days of gestation: restricted group 1 (RG1, 0.18 MJ ME · kg BW-0.75 · day-1, n = 6), restricted group 2 (RG2, 0.33 MJ ME · kg BW-0.75 · day-1, n = 6), and a control group (CG, ad libitum, 0.67 MJ ME · kg BW-0.75 · day-1, n = 6). Fetal kidney samples were collected on Day 140 of pregnancy. The results indicated that maternal nutritional restriction significantly decreased fetal kidney weight (p < 0.05) and total DNA content (p < 0.01). Masson staining showed an increase in total collagen fibers and a decrease in collagen I levels (p < 0.01). In the apoptotic pathway, nutritional restriction up-regulated the expression of proapoptotic protein p53 and Bax (p < 0.01) and activated Caspase-3 (p < 0.05) and cytochrome C (p < 0.01). It is suggested that maternal nutritional restriction in late pregnancy may impair normal fetal kidney development by interfering with extracellular matrix metabolic balance and activating p53-mediated mitochondrial apoptosis pathway. This provides important evidence for understanding the molecular mechanism of maternal undernutrition-related kidney development programming, improving ruminant reproductive efficiency, and exploring the developmental origin of human-related diseases.","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"97 1","pages":"e70173"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147466601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing Berseem (Trifolium alexandrinum L.) Ensiled With Molasses by Bioactive Agents: Effects on Ensiling Characteristics, Aerobic Stability, Rumen Fermentation, and Characteristics In Vitro. 增强Berseem （Trifolium alexandrinum L.）生物活性物质对糖蜜青贮特性、好氧稳定性、瘤胃发酵及体外特性的影响

IF 1.6 3区农林科学

Animal Science Journal Pub Date : 2026-01-01 DOI: 10.1111/asj.70229

Mariam G Ahmed, Samir Z El-Zarkouny, Eman A Elwakeel

{"title":"Enhancing Berseem (Trifolium alexandrinum L.) Ensiled With Molasses by Bioactive Agents: Effects on Ensiling Characteristics, Aerobic Stability, Rumen Fermentation, and Characteristics In Vitro.","authors":"Mariam G Ahmed, Samir Z El-Zarkouny, Eman A Elwakeel","doi":"10.1111/asj.70229","DOIUrl":"10.1111/asj.70229","url":null,"abstract":"This study investigated the effects of plant-derived bioactive compounds on the fermentation characteristics and aerobic stability of berseem (Trifolium alexandrinum) silage and evaluated their impact on ruminal fermentation. In Experiment 1, curcumin, resveratrol, eugenol, limonene, and thymol were applied at 400 and 800 mg/kg fresh matter in combination with molasses (50 g/kg) and ensiled for 60 days to screen their effects on silage quality. Bioactive supplementation modified fermentation in a compound- and dose-dependent manner, reflecting differences in physicochemical properties and antimicrobial selectivity. Among the tested compounds, eugenol was the most effective in enhancing silage fermentation and aerobic stability, as it reduced pH, increased lactic acid concentration (p < 0.05), and maintained Flieg's score comparable to the control. Experiment 2 evaluated the ruminal consequences of eugenol-treated silage using in vitro incubation at 24 and 48 h. Eugenol supplementation reduced nutrient degradability and microbial protein synthesis at 48 h and decreased methane production at both incubation times (p < 0.05). Overall, eugenol showed promising effects for improving silage preservation and aerobic stability; however, it reduced in vitro nutrient degradability and microbial protein synthesis, suggesting that optimization of inclusion strategies is required to minimize potential negative effects on ruminal nutrient utilization.","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"97 1","pages":"e70229"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13429269/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148663408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Presence Effect of Trueperella pyogenes in the Uterus at 2 Weeks Postpartum on the Progression of Cytological Endometritis and Ultrasonographic Findings in Dairy Cows. 产后2周子宫内存在化脓性真假杆菌对奶牛子宫内膜炎进展及超声表现的影响。

IF 1.6 3区农林科学

Animal Science Journal Pub Date : 2026-01-01 DOI: 10.1111/asj.70215

Yoshiyuki Tsuchiya, Sayaka Ohashi, Aiko Yamamoto-Kinami, Atsushi Kimura, Danil Kim, Toshihiro Ichijo, Kazuhiro Bandai, Ryotaro Miura

{"title":"The Presence Effect of Trueperella pyogenes in the Uterus at 2 Weeks Postpartum on the Progression of Cytological Endometritis and Ultrasonographic Findings in Dairy Cows.","authors":"Yoshiyuki Tsuchiya, Sayaka Ohashi, Aiko Yamamoto-Kinami, Atsushi Kimura, Danil Kim, Toshihiro Ichijo, Kazuhiro Bandai, Ryotaro Miura","doi":"10.1111/asj.70215","DOIUrl":"10.1111/asj.70215","url":null,"abstract":"We investigated the degree of endometrial inflammation and the fluid in the uterus (FIU) scores in Holstein cows with or without intrauterine Trueperella pyogenes infection. Scoring of FIU by transrectal ultrasonography, percentage of polymorphonuclear neutrophils (PMN%) measurement, and bacterial species identification in endometrial samples were performed at 2, 5, and 7 weeks postpartum. Threshold for cytological endometritis based on PMN% was set at ≥ 6% and ≥ 4% at 5 and 7 weeks postpartum, respectively. Cows were categorized into the T. pyogenes-positive (Tp group, n = 25) and T. pyogenes-negative groups (non-Tp group, n = 24) based on the presence of intrauterine T. pyogenes infection at 2 weeks postpartum. The prevalence of cytological endometritis at 5 and 7 weeks postpartum was significantly (p < 0.01) higher in the Tp group than in the non-Tp group (84.0% vs. 16.7% at 5, and 68.0% vs. 9.1% at 7 weeks postpartum). The Tp group exhibited significantly (p < 0.05) higher FIU scores and PMN% at 5 and 7 weeks postpartum than the non-Tp group. Intrauterine T. pyogenes infection at 2 weeks postpartum might be associated with high PMN%, cytological endometritis prevalence, and FIU scores during the postpartum period.","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"97 1","pages":"e70215"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13348505/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148410279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to "5-Aminolevulinic Acid Improves Immune Response in Dairy Cows In Vivo and In Vitro". 对“5-氨基乙酰丙酸提高奶牛体内和体外免疫反应”的更正。

IF 1.6 3区农林科学

Animal Science Journal Pub Date : 2026-01-01 DOI: 10.1111/asj.70234

引用次数: 0

Phenotypic Definition Influences Genetic Inference and Predictive Ability for Reining Performance in Quarter Horses Using Random Regression Models. 利用随机回归模型，表型定义影响四分之一马驾驭性能的遗传推断和预测能力。

IF 1.6 3区农林科学

Animal Science Journal Pub Date : 2026-01-01 DOI: 10.1111/asj.70223

Mário Luiz Santana, Annaiza Braga Bignardi

{"title":"Phenotypic Definition Influences Genetic Inference and Predictive Ability for Reining Performance in Quarter Horses Using Random Regression Models.","authors":"Mário Luiz Santana, Annaiza Braga Bignardi","doi":"10.1111/asj.70223","DOIUrl":"10.1111/asj.70223","url":null,"abstract":"Reining performance is influenced by subjectivity and competition structure, challenging genetic evaluation. This study estimated genetic parameters for reining performance in Brazilian Quarter Horses using random regression models (RRM) and evaluated how alternative phenotypic definitions affect genetic inference and predictive ability. A dataset comprising 21,165 longitudinal records from 1603 horses was analyzed, comparing within-group rank (RANK) and Blom-transformed rank (BLOM). Model comparison indicated that cubic RRM provided the best fit for both phenotypes. Heritability estimates were low and age-dependent, ranging from 0.01 to 0.17 for RANK and from 0.06 to 0.23 for BLOM. Permanent environmental and rider effects accounted for substantial phenotypic variance. Genetic correlations were higher between adjacent ages and declined across distant ages. RANK showed greater additive genetic stability across ages and slightly higher theoretical accuracy for total EBV among selected stallions. In contrast, BLOM showed greater consistency for the combined additive genetic + permanent environmental component, stronger favorable genetic trends, and better LR-based predictive performance across several age classes. Overall, both rank-based phenotypes were useful and complementary for genetic evaluation of reining performance. BLOM showed modest advantages for several criteria, whereas RANK retained relevant information for representing relative competitive performance on the observed scale.","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"97 1","pages":"e70223"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13392984/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148560821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glycosylated Carbonated Apatite Particles for Macrophage-Targeted Delivery of CpG Oligodeoxynucleotides From Streptococcus thermophilus Enhance Immune Activation. 糖基化碳酸磷灰石颗粒用于巨噬细胞靶向递送嗜热链球菌CpG寡脱氧核苷酸增强免疫激活。

IF 1.6 3区农林科学

Animal Science Journal Pub Date : 2026-01-01 DOI: 10.1111/asj.70209

Md Rayhan Chowdhury, Mitsuaki Goto, Ariful Islam, Takeshi Shimosato

{"title":"Glycosylated Carbonated Apatite Particles for Macrophage-Targeted Delivery of CpG Oligodeoxynucleotides From Streptococcus thermophilus Enhance Immune Activation.","authors":"Md Rayhan Chowdhury, Mitsuaki Goto, Ariful Islam, Takeshi Shimosato","doi":"10.1111/asj.70209","DOIUrl":"10.1111/asj.70209","url":null,"abstract":"Immunostimulatory CpG oligodeoxynucleotides (CpG-ODNs) are potent activators of innate immunity with promising applications in human and veterinary medicine. We previously developed carbonate apatite-based oligonucleotide particles (ODNcaps) for oral delivery, which are efficiently taken up by macrophages in jejunal Peyer's patches. To enhance macrophage-specific targeting, we introduced glycan modifications to ODNcaps, exploiting carbohydrate-binding receptors on macrophages. In this study, glycosylated ODNcaps were synthesized through N-acetylglucosamine (GlcNAc) conjugation and evaluated for cellular uptake and immunostimulatory activity in mouse peritoneal macrophages. Uptake efficiency was quantified using fluorescently labeled CpG-ODNs, and immune activation was assessed by IL-6 mRNA expression and IL-6 secretion. GlcNAc-modified ODNcaps showed markedly higher uptake and induced the strongest IL-6 responses at both transcriptional and protein levels compared with non-glycosylated controls. These results demonstrate that glycosylation is a simple yet effective strategy to improve macrophage targeting and immunostimulation by CpG-ODNs. Beyond murine models, this platform may hold promise for oral or mucosal immune modulation in livestock such as cattle, poultry, and swine, offering a novel approach to enhance antigen-presenting cell activation and strengthen mucosal immunity in animal production.","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"97 1","pages":"e70209"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148306549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Light and Stocking Density Regimen on Welfare and Zoo-Technical Performance of Broilers Under Tropical Conditions. 光照和放养密度方案对热带条件下肉鸡福利和动物园技术性能的影响

IF 1.2 3区农林科学

Animal Science Journal Pub Date : 2026-01-01 DOI: 10.1111/asj.70165

Sruthy Ravi, Jaydip Jaywant Rokade, Subrat Kumar Bhanja, Gautham Kolluri, Gopi Marappan, Ashok Kumar Tiwari, Mohammed Shameeh, Monika Madheshwaran, Vivek Srivastava

{"title":"Effect of Light and Stocking Density Regimen on Welfare and Zoo-Technical Performance of Broilers Under Tropical Conditions.","authors":"Sruthy Ravi, Jaydip Jaywant Rokade, Subrat Kumar Bhanja, Gautham Kolluri, Gopi Marappan, Ashok Kumar Tiwari, Mohammed Shameeh, Monika Madheshwaran, Vivek Srivastava","doi":"10.1111/asj.70165","DOIUrl":"10.1111/asj.70165","url":null,"abstract":"This study was conducted to evaluate the effect of stocking density (SD) under three different colored light-emitting diodes (LEDs) on production and welfare in tropical conditions. A total of 1224-day-old straight-run broiler chicks were divided into nine groups, each with four replicates, and housed in light-proof rooms for a period of 42 days. They were raised under three different SDs: 12 kg/m2 (26 chicks/replicate), 15 kg/m2 (33 chicks/replicate), and 20 kg/m2 (43 chicks/replicate), and three LED light colors: white (W), blue (B), and green (G). The production, welfare parameters, and litter material quality were assessed. Body weight and gait score were improved (p < 0.05) in low SD birds, but the main effect of LED light was inappreciable on growth parameters. The main effect of light color and SD had no significant difference (p > 0.05) on welfare indices. However, there was a significant interaction effect of light color and SD on the feather score of birds in this study. Similarly, light color had a significant (p < 0.05) influence on the globulin content, and the highest content was observed in the W group, followed by the G and B groups. The litter moisture content increased significantly (p < 0.05) as the SD increased from 12 to 20 kg/m2. The study concluded that birds under W color LED light and low SD could attain better growth without impairing welfare.","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"97 1","pages":"e70165"},"PeriodicalIF":1.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147300800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Estimation of the Association Between the Rumen Bacterial Abundance and Host Phenotypes of Lactating Dairy Cows Using Structural Equation Modeling. 利用结构方程模型估计泌乳奶牛瘤胃细菌丰度与宿主表型的关系

IF 1.6 3区农林科学

Animal Science Journal Pub Date : 2026-01-01 DOI: 10.1111/asj.70172

Shuhei Takizawa, Tomohisa Tomaru, Kenji Hashiba, Miho Fujimori, Takumi Shinkai, Fuminori Terada

{"title":"Estimation of the Association Between the Rumen Bacterial Abundance and Host Phenotypes of Lactating Dairy Cows Using Structural Equation Modeling.","authors":"Shuhei Takizawa, Tomohisa Tomaru, Kenji Hashiba, Miho Fujimori, Takumi Shinkai, Fuminori Terada","doi":"10.1111/asj.70172","DOIUrl":"10.1111/asj.70172","url":null,"abstract":"This study aimed to determine the association between the rumen bacterial abundance and the host phenotypes of dairy cows by performing structural equation modeling (SEM). Two herds of milking Holstein cows in Hokkaido and Gunma prefectures in Japan were examined. To improve the goodness-of-fit of the model, rumen bacteria were categorized into four groups based on the relative abundance in rumen microbiota and their correlation with (acetate + butyrate)/propionate ratio (NGR) as an indicator for rumen fermentation and enteric methane production. The top three most abundant archaeal operational taxonomic units were used as variables. Rumen fermentation parameters and blood metabolites were respectively transformed into three principal components (PCs). The SEM model revealed the interaction of the methane intensity with energy-corrected milk (ECM) and rumen PC1 and PC2 representing NGR and acetate concentration, and their standardized regression coefficients were comparable. Several bacteria that produce propionate and its intermediates, such as Prevotella lacticifex, Segatella baroniae, Parafannyhessea umbonata, and Dialister succinatiphilus, were detected in bacterial clusters interacting with rumen PC1 and PC2. Our findings indicated that ECM and several propionate- and its intermediate-producing bacteria contributed to reduce methane intensity; however, the archaea and blood PCs did not fit the SEM model.","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"97 1","pages":"e70172"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147631915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0