Re-Vitrification at the Pronuclear and Blastocyst Stage of Porcine Embryos Obtained From Vitrified Immature Oocytes

IF 1.2 3区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Science Journal Pub Date : 2025-09-11 DOI:10.1111/asj.70103

Hiep Thi Nguyen, Michiko Nakai, Shoichiro Sembon, Nguyen Xuan Bui, Uoc Thi Nguyen, Kazuhiro Kikuchi, Seiki Haraguchi, Linh Viet Nguyen, Hong Thi Nguyen, Nhung Thi Nguyen, Hanh Van Nguyen, Duc Tien Luu, Tamás Somfai

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引用次数: 0

Abstract

The aims of this study were to investigate the effects of re-vitrification at the pronuclear (PN) stage of porcine embryos generated from vitrified oocytes on subsequent development and to clarify if re-vitrification is more feasible at the PN stage or at the blastocyst stage. Immature porcine oocytes at the germinal vesicle (GV) stage were vitrified/warmed and subjected to in vitro maturation, parthenogenetic activation (PA), and embryo culture. Subsequent parthenotes were either cultured without re-vitrification for 6 days (GV-vit group) or were re-vitrified 8 h after PA at the PN stage (GV-vit/PN-revit group), and after warming, cultured for 6 days. Embryo development to the blastocyst stage was compared with a control group processed without vitrification at any stage. Then, blastocysts obtained in each group were vitrified/warmed and their survival was assessed. Blastocyst formation in the GV-vit/PN-revit group decreased dramatically (p < 0.05) compared with the GV-vit and control groups (3.4% vs. 10.2% and 22.4%, respectively). However, 80.0% of the blastocysts in the GV-vit group survived re-vitrification. Hence, after blastocyst re-vitrification, 8.2% of the vitrified oocytes re-expanded to transferable embryos. In conclusion, re-vitrification at the PN stage was detrimental for subsequent development, whereas that at the blastocyst stage was more advantageous.

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猪未成熟卵母细胞在原核和囊胚期的再玻璃化。

本研究的目的是研究由玻璃化卵母细胞产生的猪胚胎在原核（PN）阶段再玻璃化对随后发育的影响，并阐明在原核阶段还是囊胚阶段再玻璃化更可行。将生发囊（GV）期的未成熟猪卵母细胞玻璃化/加热，并进行体外成熟、孤雌生殖激活（PA）和胚胎培养。随后的孤雌体要么不进行玻璃化培养6天（GV-vit组），要么在PN期PA后8 h进行玻璃化培养（GV-vit/PN-revit组），加热后培养6天。将胚胎发育至囊胚期与未进行玻璃化处理的对照组进行比较。然后，将各组囊胚玻璃化/加热，并评估其存活率。GV-vit/PN-revit组囊胚形成显著减少(p

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来源期刊

Animal Science Journal 生物-奶制品与动物科学

CiteScore

3.80

自引率

5.00%

发文量

111

审稿时长

1 months

期刊介绍： Animal Science Journal (a continuation of Animal Science and Technology) is the official journal of the Japanese Society of Animal Science (JSAS) and publishes Original Research Articles (full papers and rapid communications) in English in all fields of animal and poultry science: genetics and breeding, genetic engineering, reproduction, embryo manipulation, nutrition, feeds and feeding, physiology, anatomy, environment and behavior, animal products (milk, meat, eggs and their by-products) and their processing, and livestock economics. Animal Science Journal will invite Review Articles in consultations with Editors. Submission to the Journal is open to those who are interested in animal science.