农林科学最新文献

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Effect of lipopolysaccharides from pathogenic bacteria on broiler chickens' productivity: a meta-analysis. 病原菌脂多糖对肉鸡生产率的影响:一项荟萃分析。
IF 1.6 3区 农林科学
British Poultry Science Pub Date : 2024-12-01 Epub Date: 2024-06-28 DOI: 10.1080/00071668.2024.2364331
H L Sanjaya, B P Maharani, A P Baskara, Muhlisin, R Martien, Zuprizal
{"title":"Effect of lipopolysaccharides from pathogenic bacteria on broiler chickens' productivity: a meta-analysis.","authors":"H L Sanjaya, B P Maharani, A P Baskara, Muhlisin, R Martien, Zuprizal","doi":"10.1080/00071668.2024.2364331","DOIUrl":"10.1080/00071668.2024.2364331","url":null,"abstract":"<p><p>1. This meta-analysis investigated the impact of LPS and covariates (serotype, rearing period and administration route) on the productivity parameters of broiler chickens (average daily feed intake (ADFI), average daily gain (ADG) and feed conversion ratio (FCR)).2. Thirty-two eligible studies were included. Hedges' g effect size was determined using a random-effects model at 95% confidence interval.3. Results showed that LPS significantly decreased average daily feed intake (ADFI; <i>p</i> < 0.0001) and average daily gain (ADG; <i>p</i> < 0.0001) and increased FCR (<i>p</i> < 0.0001). The serotypes <i>Escherichia coli</i> 055: B5 (EC055: B5) and <i>Escherichia coli</i> 0127: B8 (EC 0127: B8) significantly reduced ADFI and ADG, and the serotype EC 055: B5 significantly increased the FCR (<i>p</i> < 0.05).4. The intraperitoneal administration of the LPS significantly reduced the productivity of broiler chickens (<i>p</i> < 0.05), but other administration routes did not show such effects. The reduction in ADFI and ADG was found in all rearing periods (<i>p</i> < 0.05), and the increase in FCR was observed in the starter (<i>p</i> = 0.0302) and grower periods (<i>p</i> = 0.0031).5. Exposure to LPS significantly reduced the productivity of broiler chickens (<i>p</i> < 0.05). However, no relationship was observed between LPS dosage and productivity as indicated by the meta-regression study.6. The findings indicated that LPS has detrimental effects on broiler chickens' ADFI, ADG and FCR across various LPS serotypes and rearing periods. These detrimental impacts of LPS remain consistent regardless of the administered dosage.</p>","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"708-721"},"PeriodicalIF":1.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141466322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of ear cleaning on treatment outcome for canine otitis externa. 洗耳对犬外耳道炎治疗效果的影响
IF 1.9 3区 农林科学
Veterinary dermatology Pub Date : 2024-12-01 Epub Date: 2024-08-30 DOI: 10.1111/vde.13292
Erin Corb, Craig E Griffin, Willie Bidot, Melissa Hall, Allison Kirby, Wayne Rosenkrantz
{"title":"Effect of ear cleaning on treatment outcome for canine otitis externa.","authors":"Erin Corb, Craig E Griffin, Willie Bidot, Melissa Hall, Allison Kirby, Wayne Rosenkrantz","doi":"10.1111/vde.13292","DOIUrl":"10.1111/vde.13292","url":null,"abstract":"<p><strong>Background: </strong>Ear cleaning is often recommended for management of canine otitis externa (OE). Few in vivo studies evaluate how ear cleaning affects treatment outcome.</p><p><strong>Hypothesis/objectives: </strong>To determine the effect of ear cleaning on canine OE by comparing treatment outcome in cleaned versus noncleaned ears.</p><p><strong>Animals: </strong>Twenty-three client-owned dogs with ceruminous or purulent OE.</p><p><strong>Materials and methods: </strong>Forty ears were randomised to one of two groups: 20 ears were wiped with dry or saline-moistened gauze (grp1), and 20 external ear canals received manual cleaning (grp2) with a commercial product. One millilitre of a commercial otic suspension containing hydrocortisone aceponate, miconazole nitrate and gentamicin sulfate was applied to affected ears every 24 h for five days. Cytological scores, modified otitis index score (OTIS3), pruritus Visual Analog Scale (PVAS), modified PVAS and client questionnaire were compared on Day (D)0 and D7.</p><p><strong>Results: </strong>There were no statistical differences between cytological scores, modified OTIS3, PVAS, modified PVAS or client assessments between groups at D7. Both groups had significant reduction in all treatment parameters from D0 to D7 with the exception of cytological rod scores that were only significantly decreased in cleaned ears. There was a significant difference in OTIS3 before and after cleaning on D0.</p><p><strong>Conclusions and clinical relevance: </strong>Both groups achieved successful outcome regardless of cleaning. Ear cleaning may be more important when rod-shaped bacteria are present.</p>","PeriodicalId":23599,"journal":{"name":"Veterinary dermatology","volume":" ","pages":"716-725"},"PeriodicalIF":1.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142112511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Gastrointestinal Health Benefits of Sorghum Phenolics. 高粱酚类化合物对胃肠道健康的益处。
IF 3.1 2区 农林科学
Plant Foods for Human Nutrition Pub Date : 2024-12-01 Epub Date: 2024-08-30 DOI: 10.1007/s11130-024-01230-6
Ibtesam Sleem, Dmitriy Smolensky, Vermont Dia
{"title":"Gastrointestinal Health Benefits of Sorghum Phenolics.","authors":"Ibtesam Sleem, Dmitriy Smolensky, Vermont Dia","doi":"10.1007/s11130-024-01230-6","DOIUrl":"10.1007/s11130-024-01230-6","url":null,"abstract":"<p><p>Sorghum is considered a promising food security crop and remarkable rich source of bioactive components including phenolic acids, flavonoids, and tannins. Sorghum phenolics exhibited numerous protective effects against multiple chronic diseases. However, there is no review of the effects of sorghum phenolics on gastrointestinal (GI) health. Specifically, recent studies have highly suggested that sorghum phenolics can maintain gastrointestinal homeostasis and enhance microbial diversity and richness. Furthermore, sorghum phenolics showed GI anticancer effects in both in vitro and in vivo studies against colorectal and esophageal cancers. Treatment of GI related human cancer cell lines stimulated apoptosis and suppressed proliferation. Sorghum intake and extracts treatments reduced intestinal oxidative stress and inflammatory mediators in human and in vivo studies. In addition, understanding the role and mechanisms underlying gastrointestinal health benefits of sorghum phenolics is crucial to determine treatment strategies of different GI diseases.</p>","PeriodicalId":20092,"journal":{"name":"Plant Foods for Human Nutrition","volume":" ","pages":"730-743"},"PeriodicalIF":3.1,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142110840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In vitro efficacy of lavender oil, otological gel and gentamicin to eradicate biofilm produced by Pseudomonas aeruginosa. 薰衣草精油、耳用凝胶和庆大霉素对消除铜绿假单胞菌产生的生物膜的体外疗效。
IF 1.9 3区 农林科学
Veterinary dermatology Pub Date : 2024-12-01 Epub Date: 2024-08-30 DOI: 10.1111/vde.13294
Ana Mourão, Isa Serrano, Eva Cunha, Luís Tavares, Ana Lourenço, Manuela Oliveira
{"title":"In vitro efficacy of lavender oil, otological gel and gentamicin to eradicate biofilm produced by Pseudomonas aeruginosa.","authors":"Ana Mourão, Isa Serrano, Eva Cunha, Luís Tavares, Ana Lourenço, Manuela Oliveira","doi":"10.1111/vde.13294","DOIUrl":"10.1111/vde.13294","url":null,"abstract":"<p><strong>Background: </strong>Otitis externa (OE) is one of the most frequently diagnosed dermatological diseases in dogs, having a multifactorial aetiology. Among the bacterial agents associated with canine OE, Pseudomonas aeruginosa is of special concern owing to its frequent multidrug resistance profile and ability to form biofilms related to the infection's chronicity and recurrence.</p><p><strong>Objectives: </strong>The main objective of this study was to evaluate and compare the antibiofilm activity of two innovative antimicrobials-an otological gel containing a synthetic antimicrobial peptide and Lavandula angustifolia essential oil-with gentamicin (a conventional antibiotic) using biofilm-producing P. aeruginosa isolates obtained from dogs with OE.</p><p><strong>Materials and methods: </strong>The biofilm eradication capacity of gentamicin, otological gel and lavender oil was determined against a collection of 12 P. aeruginosa biofilm-producers among 35 clinical isolates obtained from the ear canals of dogs with OE. Also, the antimicrobial activity of the otological gel against P. aeruginosa biofilms was assessed in an in vitro model of dog cerumen.</p><p><strong>Results: </strong>Lavender oil showed the best effectiveness after 30 min of contact, eradicating 58.3% (seven of 12) of the isolates, and gentamicin showed full eradication (12 of 12) after 24 h. The otological gel acted more slowly than the lavender oil; yet at 24 h, the antibiofilm capacity of both compounds was similar, with no significant difference between them. It also was found that triglycerides from synthetic cerumen earwax had antipseudomonal activity and, when combined with the otological gel, led to the full eradication of P. aeruginosa.</p><p><strong>Conclusions and clinical relevance: </strong>The results of this in vitro study indicate that lavender oil and the otological gel may be effective topical treatments for canine OE promoted by P. aeruginosa biofilm-producers, as alternatives to gentamicin.</p>","PeriodicalId":23599,"journal":{"name":"Veterinary dermatology","volume":" ","pages":"726-735"},"PeriodicalIF":1.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142112513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Minimum inhibitory and bactericidal/fungicidal concentration of commercially available products containing essential oils, zinc gluconate, or 4% chlorhexidine for Malassezia pachydermatis, Pseudomonas aeruginosa, and multi-drug resistant Staphylococcus pseudintermedius canine clinical isolates. 含有精油、葡萄糖酸锌或 4% 洗必泰的市售产品对犬临床分离物马拉色菌、铜绿假单胞菌和耐多药假中间葡萄球菌的最小抑菌和杀菌/杀真菌浓度。
IF 1.8 3区 农林科学
Veterinary Research Communications Pub Date : 2024-12-01 Epub Date: 2024-09-03 DOI: 10.1007/s11259-024-10528-4
Alexandra Bergen, Savannah Roemhild, Domenico Santoro
{"title":"Minimum inhibitory and bactericidal/fungicidal concentration of commercially available products containing essential oils, zinc gluconate, or 4% chlorhexidine for Malassezia pachydermatis, Pseudomonas aeruginosa, and multi-drug resistant Staphylococcus pseudintermedius canine clinical isolates.","authors":"Alexandra Bergen, Savannah Roemhild, Domenico Santoro","doi":"10.1007/s11259-024-10528-4","DOIUrl":"10.1007/s11259-024-10528-4","url":null,"abstract":"<p><p>Skin infections are common complications in both humans and animals. Because of the increased incidence of multi-drug resistant (MDR) skin infections, essential oils have been suggested as potential alternatives to the classic antimicrobials. The goal of this study was to evaluate the minimum inhibitory and bactericidal/fungicidal concentrations (MIC and MBC/MFC) of commercially available products containing essential oils, zinc gluconate, or 4% chlorhexidine. Microbroth dilution technique was performed on clinical isolates of MDR Staphylococcus pseudintermedius (MDR-SP; n = 10), Pseudomonas aeruginosa (PA; n = 10), and Malassezia pachydermatis (MP; n = 10). For MDR-SP, essential oil-containing products showed median MICs of 1:240 and 1:320. The chlorhexidine shampoo had a MIC of 1:128,000 (0.312 µg/mL), whereas zinc gluconate products had median MICs of 1:320 and 1:160. Three essential oil-containing shampoos (MBC 1:40), the zinc gluconate (MBC 1:40), and the chlorhexidine (MBC 1:64,000 [0.625 µg/mL]) reached an MBC. For PA, essential oil-containing products showed median MICs of 1:30 and 1:80. The zinc-gluconate products had a median MIC of 1:160, whereas the chlorhexidine shampoo had a median MIC of 1:4,000 (10 µg/mL). Only the zinc-gluconate products (MBC 1:80) and the chlorhexidine shampoo (MBC 1:2,000 [20 µg/mL]) reached an MBC. For MP, essential oil-containing and zinc-gluconate products showed lower median MICs (1:4,800 and 7,200) for shampoos compared with other formulations (1:160 and 1:320), whereas the chlorhexidine shampoo had a median MIC of 1:80,000 (0.5 µg/mL). These results suggest that natural topical compounds can be an effective alternative to treat skin infections in companion animals. Further in vivo studies are needed to clinically confirm this study's results.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":" ","pages":"3699-3709"},"PeriodicalIF":1.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142120699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antioxidant properties of D-limonene and its nanoemulsion form enhance its anticoccidial efficiency in experimentally infected broilers with Eimeria tenella: an in vitro and in vivo study. D-柠檬烯及其纳米乳液的抗氧化特性提高了其在实验性感染天牛埃默氏菌的肉鸡中的抗球虫效率:一项体外和体内研究。
IF 1.8 3区 农林科学
Veterinary Research Communications Pub Date : 2024-12-01 Epub Date: 2024-09-05 DOI: 10.1007/s11259-024-10512-y
Osama Ewais, Heba Abdel-Tawab, Huda El-Fayoumi, Shawky M Aboelhadid, Saleh Al-Quraishy, Piotr Falkowski, Abdel-Azeem S Abdel-Baki
{"title":"Antioxidant properties of D-limonene and its nanoemulsion form enhance its anticoccidial efficiency in experimentally infected broilers with Eimeria tenella: an in vitro and in vivo study.","authors":"Osama Ewais, Heba Abdel-Tawab, Huda El-Fayoumi, Shawky M Aboelhadid, Saleh Al-Quraishy, Piotr Falkowski, Abdel-Azeem S Abdel-Baki","doi":"10.1007/s11259-024-10512-y","DOIUrl":"10.1007/s11259-024-10512-y","url":null,"abstract":"<p><p>The excessive use of conventional medications to treat coccidiosis has led to concerns regarding drug residues in tissues and the emergence of multidrug resistance. Essential oils with anti-inflammatory and antioxidant activities may also have anticoccidial effects. The present study investigated the efficacy of D-limonene and its nanoemulsion form against Eimeria tenella in chickens. An in vitro study was conducted to evaluate the sporulation inhibitory effects of D-limonene on Eimeria tenella oocysts. Five D-limonene concentrations (0.625, 1.25, 2.5, 5, and 10% v/v) were tested alongside positive (10% formalin) and negative (2.5% potassium dichromate) controls. Each ELISA plate well was inoculated with 1200 unsporulated oocysts and incubated at 30 °C for 24, 48, and 72 h. Subsequently, samples were microscopically examined to assess sporulation inhibition and calculate the percentage of sporulated oocysts. For the in vivo study, 125 eight-day-old broiler chicks were divided into five groups of 25 birds each. The control negative group remained uninfected and untreated. The control positive group was challenged with 5 × 10<sup>4</sup> sporulated Eimeria tenella oocysts. The diclazuril group received 0.2 mg/kg diclazuril in their diet two days prior to, and until 10 days post infection. The D-limonene (DL) and D-limonene nanoemulsion (DLN) groups were challenged with 5 × 10<sup>4</sup> sporulated E. tenella oocysts at 18 days of age and administered 150 mg/L of their respective treatments in drinking water from day eight until the end of the experiment. Results from the in vitro study demonstrated that D-limonene suppressed oocyst sporulation by 50.83% at its highest concentration of 10%. In the in vivo study, both DL and DLN treated groups exhibited a significant reduction in oocyst output per gram of feces (OPG), along with increased body weight and decreased parasite stages in the cecal tissue. Furthermore, these treatments were associated with elevated levels of antioxidant enzymes such as glutathione peroxidase (GPX), catalase (CAT), and superoxide dismutase (SOD), accompanied by a decrease in malondialdehyde (MDA) and nitric oxide (NO) levels. Particularly, DLN treatment remarkably increased the number of goblet cells. In conclusion, D-limonene and its nanoemulsion represent promising alternatives for managing coccidiosis in poultry. They not only effectively control parasites but also promote intestinal health and boost antioxidant defenses.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":" ","pages":"3711-3725"},"PeriodicalIF":1.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142133923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Investigation of the biological activity and toxicity of bioactive silver nanoparticles synthesized via Vitex agnus-castus seed extract on honey bees. 通过荆芥种子提取物合成的生物活性银纳米粒子对蜜蜂的生物活性和毒性研究。
IF 1.8 3区 农林科学
Veterinary Research Communications Pub Date : 2024-12-01 Epub Date: 2024-09-21 DOI: 10.1007/s11259-024-10550-6
Seda Ekici, Ogun Bozkaya, Sedat Sevin, Babur Erdem, Okan Can Arslan, Ozge Özgenç Cinar, Esra Bozkaya, Husamettin Ekici
{"title":"Investigation of the biological activity and toxicity of bioactive silver nanoparticles synthesized via Vitex agnus-castus seed extract on honey bees.","authors":"Seda Ekici, Ogun Bozkaya, Sedat Sevin, Babur Erdem, Okan Can Arslan, Ozge Özgenç Cinar, Esra Bozkaya, Husamettin Ekici","doi":"10.1007/s11259-024-10550-6","DOIUrl":"10.1007/s11259-024-10550-6","url":null,"abstract":"<p><p>In this study, silver nanoparticles (AgNPs) coated with bioactive molecules were synthesized via Vitex agnus-castus L. (VAC) seed extract (VAC/AgNPs). The synthesized VAC/AgNPs were characterized by surface plasmon resonance (SPR) ultraviolet-visible region spectroscopy (UV-Vis). The hydrodynamic size and surface charge analysis of the particles were measured with a Zeta sizer. The results of UV-Vis and Zeta analysis revealed that AgNPs were synthesized, the size distribution was nanoscale, and the solution was stable. The effects of the synthesized VAC/AgNPs and aqueous extract of VAC seeds on honeybees were investigated by means of lifespan test and histopathological analysis. It was determined that both VAC seed extract and VAC/AgNPs were non-toxic to honeybees at certain doses, positively affected their life span and contributed to their longevity in the life span test. Furthermore, no adverse effects were detected in terms of intestinal health in histopathological examinations. Therefore, VAC/AgNPs are considered to be a promising bioactive agent for honeybees.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":" ","pages":"3813-3821"},"PeriodicalIF":1.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antimicrobial resistance and virulence profiling of Staphylococcus pseudintermedius isolated from cats, Bangladesh. 从孟加拉国猫体内分离出的假中间葡萄球菌的抗菌药耐药性和毒力特征。
IF 6.4 2区 农林科学
Veterinary Quarterly Pub Date : 2024-12-01 Epub Date: 2024-03-15 DOI: 10.1080/01652176.2024.2326848
Eaftekhar Ahmed Rana, Tanvir Ahmad Nizami, Md Sayedul Islam, Subrata Sarker, Hafizar Rahman, Azizul Hoque, Mizanur Rahman
{"title":"Antimicrobial resistance and virulence profiling of <i>Staphylococcus pseudintermedius</i> isolated from cats, Bangladesh.","authors":"Eaftekhar Ahmed Rana, Tanvir Ahmad Nizami, Md Sayedul Islam, Subrata Sarker, Hafizar Rahman, Azizul Hoque, Mizanur Rahman","doi":"10.1080/01652176.2024.2326848","DOIUrl":"10.1080/01652176.2024.2326848","url":null,"abstract":"<p><p><i>Staphylococcus pseudintermedius</i> is a significant bacterial pathogen that frequently colonizes different body sites and mucous membranes of pets. The objectives of the cross-sectional study were to estimate the prevalence, antimicrobial resistance pattern, and detection of diverse resistance as well as virulence genes of <i>S. pseudintermedius</i> in cats. A standard bacteriological method, species-specific gene and different antimicrobial resistance as well as virulence genes were confirmed by PCR assay. A total of 233 swab samples were collected from different body sites of 102 cats, among them 146 swabs from 73 healthy cats, and 87 from 29 diseased cats. Overall, prevalence of <i>S. pseudintermedius</i> in cats was 12.01%, while dermatitis and otitis affected cats were 26.08% and 33.33%, respectively. The highest antimicrobial resistance was observed against penicillin (96.42%) followed by streptomycin (85.71%) and erythromycin (78.57%). Moreover, 89.28% of <i>S. pseudintermedius</i> isolates exhibit multi-drug resistance (MDR) (≥ 3 classes' antimicrobial resistant). In addition, 17.86% isolates harbored the <i>mec</i>A gene; thus, were classified as methicillin-resistant <i>S. pseudintermedius</i> (MRSP). Furthermore, the erythromycin resistance genes <i>erm</i>A and <i>erm</i>B were harbored by 25% and 10.71% of isolates, while 42.86% and 17.86% of isolates carried <i>tet</i>K and <i>tet</i>L (tetracycline resistance) genes, respectively. In virulence profiling, 32.14% (<i>sea</i>) and 10.71% (<i>seb</i>) of isolates were found positive for enterotoxin genes, whereas, the toxic shock syndrome toxin-1 (<i>tst-</i>1) gene and the Panton-Valentine leukocidin gene (<i>pvl</i>) were detected in 25% and 14.29% of isolates, respectively. To our knowledge, this is the first report of cats in Bangladesh for MDR <i>S. pseudintermedius</i>, MRSP, and their virulence profiling.</p>","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"44 1","pages":"1-11"},"PeriodicalIF":6.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10946260/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140133198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Tertiary lymphoid organs in wild boar exposed to a low-virulent isolate of African swine fever virus. 暴露于低毒性非洲猪瘟病毒分离株的野猪的三级淋巴器官。
IF 6.4 2区 农林科学
Veterinary Quarterly Pub Date : 2024-12-01 Epub Date: 2024-03-27 DOI: 10.1080/01652176.2024.2331525
Néstor Porras, José M Sánchez-Vizcaíno, Antonio Rodríguez-Bertos, Aleksandra Kosowska, José Á Barasona
{"title":"Tertiary lymphoid organs in wild boar exposed to a low-virulent isolate of African swine fever virus.","authors":"Néstor Porras, José M Sánchez-Vizcaíno, Antonio Rodríguez-Bertos, Aleksandra Kosowska, José Á Barasona","doi":"10.1080/01652176.2024.2331525","DOIUrl":"10.1080/01652176.2024.2331525","url":null,"abstract":"<p><p>Despite the great interest in the development of a vaccine against African swine fever (ASF) in wild boar, the immunological mechanisms that induce animal protection are still unknown. For this purpose, tertiary lymphoid organs (TLOs) of wild boar were characterised and compared with mucosa-associated lymphoid tissues (MALTs) by histopathology, histomorphometry and immunohistochemistry (CD3, CD79, PAX5, LYVE1, fibronectin). In addition, real-time polymerase chain reaction (qPCR) and immunohistochemistry (p72) were used to evaluate the presence of ASF virus (ASFV) in blood and tissues samples, respectively. TLOs were observed in animals infected with a low-virulent ASFV isolate (LVI), animals co-infected with low and high-virulent ASFV isolates (LVI-HVI) and animals infected only with the high virulence isolate (HVI). TLOs in LVI and LVI-HVI groups were located adjacent to the mucosa and presented a similar structure to MALT. Immunoexpresion of p72 observed in the inflammatory cells adjacent to TLOs/MALTs confirmed its development and reactivity generated by ASF attenuated isolates. Immunohistochemical evaluation, based on cellular composition (T and B lymphocytes), and histomorphometrical study revealed a more pronounced maturation of TLOs/MALTs in the LVI-HVI group. It is currently unclear whether these formations play a protective role by contributing to local immunity in chronic inflammatory diseases. However, the structural similarities between TLOs and MALTs and the location of TLOs close to the mucosa suggest that they may perform a similar function, facilitating a local protective response. Nevertheless, further investigations are warranted to assess the cellular and humoral dynamics of these lymphoid organs induced by attenuated isolates.</p>","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"44 1","pages":"1-13"},"PeriodicalIF":6.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10976996/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140295215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Knowledge, attitudes and behavior of Gulf medical students in relation to Food Dome dietary guidelines: a mixed-method research. 海湾地区医科学生对膳食指南的认识、态度和行为:一项混合方法研究。
IF 3.5 2区 农林科学
International Journal of Food Sciences and Nutrition Pub Date : 2024-12-01 Epub Date: 2024-10-14 DOI: 10.1080/09637486.2024.2415120
Alexander Woodman, Aseel AlSaleh, Nizar Jaoua, Abdel Halim Deifalla, Lamiaa H Al-Jamea
{"title":"Knowledge, attitudes and behavior of Gulf medical students in relation to Food Dome dietary guidelines: a mixed-method research.","authors":"Alexander Woodman, Aseel AlSaleh, Nizar Jaoua, Abdel Halim Deifalla, Lamiaa H Al-Jamea","doi":"10.1080/09637486.2024.2415120","DOIUrl":"10.1080/09637486.2024.2415120","url":null,"abstract":"<p><p>This mixed-methods research is the first to explore knowledge, attitudes and behaviours in relation to the Food Dome dietary guidelines (FDDG) among Gulf medical students. Quantitative phase included the administration of Food Dome questionnaire among <i>n</i> = 414 students; qualitative phase used focus group discussions. The quantitative findings show that more than half the sample reported that they had heard of the FDDG (55.3%), of which 15.7% followed and 39.6% did not follow the FDDG. An average female knew more about and behaved more in line with the guidelines, Bahrainis and Saudis were more aware than Kuwaitis on average, and those aged 21 or more showed, on average, more behaviour consistency with the FDDG. The qualitative findings show that the food choices of the Gulf medical students largely depend on time, university load, availability, and taste. Suggested recommendations include educational campaigns, FDDG application examples, teaching children from an early age.</p>","PeriodicalId":14087,"journal":{"name":"International Journal of Food Sciences and Nutrition","volume":" ","pages":"863-881"},"PeriodicalIF":3.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142464656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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