IF 1.8 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-08-31 DOI: 10.1080/10495398.2025.2547344

Deyanira Figueroa, Yolanda Romero, Lizeth A Heredia-Vilchez, Carlos Poemape, Wigoberto Alvarado, Carlos Quilcate

{"title":"Genomic analysis for cattle breeding improvement, progress and future perspectives in Peru: a review.","authors":"Deyanira Figueroa, Yolanda Romero, Lizeth A Heredia-Vilchez, Carlos Poemape, Wigoberto Alvarado, Carlos Quilcate","doi":"10.1080/10495398.2025.2547344","DOIUrl":"https://doi.org/10.1080/10495398.2025.2547344","url":null,"abstract":"Genomics offers a promising solution by enabling precise cattle selection and breeding to boost productivity and sustainability. In Peru, livestock plays a crucial role in the economy and food security. Despite its importance, the sector faces significant challenges, including poor pasture quality, limited conservation practices, a shortage of trained professionals, minimal use of genomic tools, and an incomplete understanding of the genetic potential of both native and introduced breeds. Since the 1940s, Peru has advanced in genetic improvement through artificial insemination, improved semen preservation, the establishment of a National Semen Bank, and the introduction of new breeds. Key developments have included embryo transfer, in vitro fertilization, and pioneering cloning efforts. Future perspectives for livestock genomics in Peru involve expanding bioinformatics capacity, improving genomic infrastructure, and integrating genomic selection into national breeding strategies. This review discusses the history, current status, challenges, and future perspectives of livestock genomics in Peru.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2547344"},"PeriodicalIF":1.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144939105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to "Phenolics from noni (Morinda citrifolia L.) fruit alleviate obesity in high fat diet-fed mice via modulating the gut microbiota and mitigating intestinal damage" [Food Chem. 402 (2023) 134232]. “noni （Morinda citrifolia L.）果实中的酚类物质通过调节肠道微生物群和减轻肠道损伤来减轻高脂肪饮食小鼠的肥胖”[食品化学，402(2023)134232]。

IF 9.8 1区农林科学

Food Chemistry Pub Date : 2025-11-15 Epub Date: 2025-08-22 DOI: 10.1016/j.foodchem.2025.145940

Ruimin Wang, Lu Wang, Shengwei Wang, Juntao Wang, Congyan Su, Lin Zhang, Congfa Li, Sixin Liu

引用次数: 0

Untargeted flavoromics and correlation analysis reveal microbial interactions driving flavor in LAB co-fermented Beita juice. 非靶向风味组学和相关分析揭示了LAB共发酵贝他汁中微生物相互作用的影响。

IF 5.2 1区农林科学

International journal of food microbiology Pub Date : 2025-11-02 Epub Date: 2025-08-05 DOI: 10.1016/j.ijfoodmicro.2025.111378

Yajie Zhao, Chengjie Tong, Chencheng Gu, Qinwei Xu, Weirong Yao, He Qian, Yuliang Cheng

{"title":"Untargeted flavoromics and correlation analysis reveal microbial interactions driving flavor in LAB co-fermented Beita juice.","authors":"Yajie Zhao, Chengjie Tong, Chencheng Gu, Qinwei Xu, Weirong Yao, He Qian, Yuliang Cheng","doi":"10.1016/j.ijfoodmicro.2025.111378","DOIUrl":"10.1016/j.ijfoodmicro.2025.111378","url":null,"abstract":"Lactic acid bacteria (LAB) co-fermentation has been increasingly recognized as an effective approach to improve the sensory quality of food products. In this study, a novel starter composed of Lacticaseibacillus rhamnosus (L. rhamnosus), Lactiplantibacillus plantarum (L. plantarum), and Lactobacillus helveticus (L. helveticus) was innovatively applied in co-fermentation of Beita juice, imparting distinctive flavor to the final product. The dynamic changes of key flavor substances, including organic acids (OAs), free amino acids (FAAs), and volatile flavor compounds (VFCs) during fermentation were systematically elucidated. Interaction-network and redundancy analysis (RDA) clearly demonstrated the synergistic effects of the three LAB strains on shaping the flavor profile of the final product, particularly contributing to the formation of mild acidity, umami taste, and enriched fruity-floral aroma. Untargeted metabolomics revealed a prospective impact of LAB co-fermentation on the metabolic profile of Beita juice, resulting in 4 significant differential metabolites including diacetyl, malate, l- (+)-lactic acid, and dl-malic acid out of 278 differential metabolites, consistency to the results of flavor substances analysis. And pathway enrichment analysis further confirmed that pathways associated with energy metabolism (e.g., carbohydrate metabolism) and the biosynthesis of key flavor precursors (particularly amino acid and organic acid metabolism) were significantly enriched during co-fermentation. This study provides critical insights into the biochemical mechanisms by which LAB co-fermentation enhances flavor characteristics, offering a scientific basis for targeted flavor modulation in fermented foods production.","PeriodicalId":14095,"journal":{"name":"International journal of food microbiology","volume":"442 ","pages":"111378"},"PeriodicalIF":5.2,"publicationDate":"2025-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144803982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modulating bacterial virulence: The role of food-plant essential oils in counteracting foodborne pathogen threats - A systematic review. 调节细菌毒力：食用植物精油在对抗食源性病原体威胁中的作用-系统综述。

IF 5.2 1区农林科学

International journal of food microbiology Pub Date : 2025-11-02 Epub Date: 2025-08-05 DOI: 10.1016/j.ijfoodmicro.2025.111382

Juliana Fidelis, Yago Alves de Aguiar Bernardo, Hannay Crystynah Almeida de Souza, Carlos Adam Conte-Junior, Vania Margaret Flosi Paschoalin

{"title":"Modulating bacterial virulence: The role of food-plant essential oils in counteracting foodborne pathogen threats - A systematic review.","authors":"Juliana Fidelis, Yago Alves de Aguiar Bernardo, Hannay Crystynah Almeida de Souza, Carlos Adam Conte-Junior, Vania Margaret Flosi Paschoalin","doi":"10.1016/j.ijfoodmicro.2025.111382","DOIUrl":"10.1016/j.ijfoodmicro.2025.111382","url":null,"abstract":"Pathogenic bacteria significantly threaten human health, mainly through the ingestion of contaminated food. Because of this, alternative strategies to combat this are required to guarantee food safety. In this sense, food-plant essential oils (EOs) displaying antibacterial activities have become promising natural alternatives enhancing food preservation. This review focuses on EOs mechanisms of action, such as inhibiting biofilm formation, disrupting quorum sensing, and generating reactive oxygen species. A search following PRISMA guidelines retrieved 538 articles, with 12 relevant studies selected after applying the inclusion criteria. Essential oils derived from cinnamon, citrus, rosemary, and clove display significant antibacterial and anti-biofilm activities, highlighting their potential for application in food matrices. Food-plant essential oils were shown to be very effective in controlling foodborne pathogen proliferation in chicken and poultry meats, as Listeria monocytogenes and Salmonella Heidelberg, bringing promising employment as natural preservative substances in animal-derived food matrices. These natural compounds can overcome antibiotic resistance, as they interfere with QS, exhibit antibiofilm activity, and induce oxidative stress. However, their application in foods requires careful consideration, as improper use may pose risks, including toxicity, allergenic reactions, and the development of bacterial resistance. To maximize the efficacy of EOs while mitigating resistance concerns, future research should focus on validating their effects in food systems, prioritizing in vivo studies, and exploring the use of antibacterial nanoemulsions for improved stability and performance in food applications. Co-toxicity assessments should also be conducted to evaluate potential hazardous effects of EOs in food contexts.","PeriodicalId":14095,"journal":{"name":"International journal of food microbiology","volume":"442 ","pages":"111382"},"PeriodicalIF":5.2,"publicationDate":"2025-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144816566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanistic insights into co-culture inhibition of pellicle formation in fermented chili peppers by Pediococcus ethanolidurans M1117. 乙醇化球球菌M1117共培养抑制发酵辣椒膜形成的机理研究。

IF 5.2 1区农林科学

International journal of food microbiology Pub Date : 2025-11-02 Epub Date: 2025-08-05 DOI: 10.1016/j.ijfoodmicro.2025.111377

Yumeng Li, Yue Xiao, Yanping Wu, Kai Zhong, Yina Huang, Baiyi Lu, Hong Gao

{"title":"Mechanistic insights into co-culture inhibition of pellicle formation in fermented chili peppers by Pediococcus ethanolidurans M1117.","authors":"Yumeng Li, Yue Xiao, Yanping Wu, Kai Zhong, Yina Huang, Baiyi Lu, Hong Gao","doi":"10.1016/j.ijfoodmicro.2025.111377","DOIUrl":"10.1016/j.ijfoodmicro.2025.111377","url":null,"abstract":"Pellicle formation in pickles is detrimental, yet current inhibition methods are limited, necessitating the exploration of novel strategies and underlying mechanisms. This study identified Pichia kudriavzevii M420 as the primary microorganism responsible for pellicle formation in fermented chili peppers, which significantly reduced the levels of organic acids, free amino acids, and aroma compounds. Consequently, this altered the sensory profile of pickles by diminishing sourness and sweetness, increasing bitterness, and introducing a wine-like flavor along with other undesirable odors, thereby compromising overall quality. Co-culturing with Pediococcus ethanolidurans M1117 effectively inhibited pellicle formation through microbial interactions with P. kudriavzevii M420. Transcriptome analysis revealed substantial downregulation of genes in P. kudriavzevii M420, particularly those involved in energy metabolism, genetic information processing, and cellular functions. Notably, inhibition of protein synthesis was identified as a crucial mechanism in preventing pellicle formation. These findings provide deep insights into the molecular mechanisms underlying pellicle formation and highlight the potential of P. ethanolidurans M1117 as an innovative biocontrol strategy to enhance the quality and safety of fermented chili peppers.","PeriodicalId":14095,"journal":{"name":"International journal of food microbiology","volume":"442 ","pages":"111377"},"PeriodicalIF":5.2,"publicationDate":"2025-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144799043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combined efficacy of Bacillus amyloliquefaciens CIM95 and melatonin in reducing anthracnose in strawberries (Fragaria × ananassa Duch.) by enhancing antioxidant activity and defense-related genes. 解淀粉芽孢杆菌CIM95和褪黑素通过增强草莓抗氧化活性和防御相关基因联合降低草莓炭疽病的效果

IF 5.2 1区农林科学

International journal of food microbiology Pub Date : 2025-11-02 Epub Date: 2025-08-05 DOI: 10.1016/j.ijfoodmicro.2025.111379

Anam Moosa, Arwa Abdulkreem Al-Huqail, Dikhnah Alshehri, Suliman Mohammed Suliman Alghanem, Faisal Zulfiqar, Gamal Awad El-Shaboury, Fahad Al-Asmari, Nurah M Alzamel, Layla A Alahmari, Tolga İzgü

{"title":"Combined efficacy of Bacillus amyloliquefaciens CIM95 and melatonin in reducing anthracnose in strawberries (Fragaria × ananassa Duch.) by enhancing antioxidant activity and defense-related genes.","authors":"Anam Moosa, Arwa Abdulkreem Al-Huqail, Dikhnah Alshehri, Suliman Mohammed Suliman Alghanem, Faisal Zulfiqar, Gamal Awad El-Shaboury, Fahad Al-Asmari, Nurah M Alzamel, Layla A Alahmari, Tolga İzgü","doi":"10.1016/j.ijfoodmicro.2025.111379","DOIUrl":"10.1016/j.ijfoodmicro.2025.111379","url":null,"abstract":"Anthracnose caused by Colletotrichum acutatum causes substantial losses in yield and fruit quality. This study aimed to investigate the combined effect of Bacillus-derived lipopeptides (LPs) and melatonin (MLT) on the suppression of anthracnose in strawberries. Compared with the control, ten different Bacillus species were screened for their antifungal activity in vitro, with Bacillus amyloliquefaciens CIM95 resulting in the greatest colony growth inhibition of C. acutatum, followed by B. subtilis LLCG89. LPs were extracted from the two strains, CIM95 and LLCG89, where CIM95 resulted in greater colony growth inhibition of the pathogen. Liquid chromatography time of flight mass spectrometry (LC-TOF/MS) analysis of CIM95 revealed the presence of eight different LPs; surfactin, bacillibactin, bacillaene, fengycin, bacillomycin D, bacilysin, iturin, and plantazolicin. The application of MLT on strawberry fruit at different concentrations revealed that the 5 mM concentration resulted in the lowest lesion diameter and disease incidence of anthracnose in strawberry fruit. Our findings revealed that, compared with individual application or no treatment, combined treatment with CIM95 LPs and MLT reduced the disease incidence and lesion diameter of the pathogen on strawberry fruit. Furthermore, CIM95 LPs and MLT presented the greatest increase in the levels of key antioxidant enzymes, viz., SOD, POD, CAT, PPO, PAL, APX, CHI, and GLU. Additionally, the expression of PR1, PR5, PPO, GLU APX, CAT, PAL, NPR1, GST, and CH-1 was up upregulated till 48 h and subsequently decreased to 96 h. These results suggest that the combination of CIM95 LPs and MLT represents a sustainable and eco-friendly approach for managing anthracnose in strawberries.","PeriodicalId":14095,"journal":{"name":"International journal of food microbiology","volume":"442 ","pages":"111379"},"PeriodicalIF":5.2,"publicationDate":"2025-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144812042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to "Flavonoid-rich extract of Paederia scandens (Lour.) Merrill improves hyperuricemia by regulating uric acid metabolism and gut microbiota" [Food Chem. 471 (2025) 142857]. “黄酮提取物”的勘误表美林通过调节尿酸代谢和肠道微生物群改善高尿酸血症[食品化学，471(2025)142857]。

IF 9.8 1区农林科学

Food Chemistry Pub Date : 2025-11-01 Epub Date: 2025-08-19 DOI: 10.1016/j.foodchem.2025.145859

Yuyi Liu, Shanling Sheng, Linye Wu, Huixian Wang, Hui Xue, Ruimin Wang

引用次数: 0

The effect of age on the excretion of ovine herpesvirus-2 (OvHV-2), the causative agent of malignant catarrhal fever (MCF), in naturally infected sheep. 年龄对羊疱疹病毒-2 （OvHV-2）排泄的影响，OvHV-2是恶性卡他性热（MCF）的病原体。

IF 2.4 2区农林科学

Preventive veterinary medicine Pub Date : 2025-11-01 Epub Date: 2025-08-05 DOI: 10.1016/j.prevetmed.2025.106638

Mohammad Rahim Haji Hajikolaei, Masoud Reza Seyfi Abad Shapouri, Tara Tahmasbi, Maryam Faghani, Shoroush Hasanpour, Hamzeh Ghobadian Diali

{"title":"The effect of age on the excretion of ovine herpesvirus-2 (OvHV-2), the causative agent of malignant catarrhal fever (MCF), in naturally infected sheep.","authors":"Mohammad Rahim Haji Hajikolaei, Masoud Reza Seyfi Abad Shapouri, Tara Tahmasbi, Maryam Faghani, Shoroush Hasanpour, Hamzeh Ghobadian Diali","doi":"10.1016/j.prevetmed.2025.106638","DOIUrl":"10.1016/j.prevetmed.2025.106638","url":null,"abstract":"Malignant catarrhal fever (MCF) is one of the most important viral diseases affecting cattle and buffaloes. One of the viruses associated with this disease is ovine herpesvirus 2 (OvHV-2), which is responsible for sheep-associated MCF. Sheep serve as the reservoir host for OvHV-2, transmitting the virus susceptible species such as buffaloes and cattle. Virus shedding in sheep is thought to be age-dependent. Therefore, this study aimed to investigate OvHV-2 infection in sheep and determine the age at which virus excretion occurs. For this purpose, 191 female sheep from four suburbs of Ahvaz were studied. The studied sheep were female and according to incisors teeth formula divided into six age groups; 3-< 6, 6-< 12 months to 1, 2, 3,and ≥ 4 years. Blood and nasal swab samples were taken from each animal. Buffy coats were removed from the blood samples, and DNA extraction were performed from buffy coats and swab sample. Semi-nested PCR was used to detect OvHV-2 DNA. The PCR results showed that 89.5 % of blood samples and 78.5 % of nasal swabs were positive for OvHV-2. Nasal excretion of the virus was observed across all ages groups, though the frequency varied with age. A significant correlation was found between presence of virus in blood and detection of virus in nasal swap samples. In conclusion, sheep of all ages are capable to excretion OvHV-2 through nasal secretion, posing a risk of infecting susceptible animals.","PeriodicalId":20413,"journal":{"name":"Preventive veterinary medicine","volume":"244 ","pages":"106638"},"PeriodicalIF":2.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144804618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Yorkie involves in immune response under hypoxia stress in Chinese mitten crab (Eriocheir sinensis). Yorkie参与了中华绒螯蟹（Eriocheir sinensis）缺氧胁迫下的免疫应答。

IF 3.9 2区农林科学

Fish & shellfish immunology Pub Date : 2025-11-01 Epub Date: 2025-08-06 DOI: 10.1016/j.fsi.2025.110636

Yuhan Ma, Yuchang Zhu, Chang Liu, Yiyang Xu, Zihao Wu, Yuhan Tu, Junwei Guo, Siyue Li, Yuting Xi, Jialin Li, Dazuo Yang, Rantao Zuo, Shu Huang, Qilin Yi

{"title":"Yorkie involves in immune response under hypoxia stress in Chinese mitten crab (Eriocheir sinensis).","authors":"Yuhan Ma, Yuchang Zhu, Chang Liu, Yiyang Xu, Zihao Wu, Yuhan Tu, Junwei Guo, Siyue Li, Yuting Xi, Jialin Li, Dazuo Yang, Rantao Zuo, Shu Huang, Qilin Yi","doi":"10.1016/j.fsi.2025.110636","DOIUrl":"10.1016/j.fsi.2025.110636","url":null,"abstract":"Oxygen is essential for the survival of aquatic animals. Hypoxia constitutes a critical environmental stressor that fundamentally impacts immune homeostasis in aquatic crustaceans. However, the immune regulatory mechanisms of crustaceans under hypoxia stress require further exploration. Yorkie (Yki), a key downstream effector of the Hippo signaling pathway, has been implicated in innate immunity regulation, its precise functional mechanisms during hypoxic conditions in Eriocheir sinensis remain unclear. This study investigated the expression characteristics and immune regulation of Yki in response to hypoxia stress in E. sinensis. The mRNA expression of Yki and its downstream target gene, Cactus in haemocytes were significantly increased from 3 h to 6 h post hypoxia stress, and then decreased to slightly lower than that of the control. The Yki phosphorylation level under hypoxia stress was significantly decreased. Meanwhile, the positive green signal of Yki translocated from the cytoplasm to the nucleus under hypoxia stress. The Yki and Cactus transcripts were significantly increased after Aeromonas hydrophila injection under hypoxic conditions. A. hydrophila stimulation inhibited and promoted the phosphorylation level and nuclear translocation of Yki post hypoxia stress, respectively. Furthermore, after the reduction of Yki mRNA expression by RNAi, the mRNA expression levels of lipopolysaccharide factors (ALFs) in haemocytes were significantly up-regulated post A. hydrophila stimulation under hypoxia stress, whereas they significantly decreased after the inhibition of Yki phosphorylation by CA3 injection. Our findings collectively suggested that hypoxia could activate Yki, and Yki was involved in regulating ALFs expression in E. sinensis, which enrich the understanding of regulatory mechanisms underlying immune defense in crustaceans under hypoxia stress.","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":" ","pages":"110636"},"PeriodicalIF":3.9,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144798576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quaternary ammonium cationic surfactants target Vfm quorum sensing to suppress the virulence of rice pathogen Dickeya oryzae. 季铵盐阳离子表面活性剂以Vfm群体感应为靶点抑制稻瘟病菌的毒力。

IF 4 1区农林科学

Pesticide Biochemistry and Physiology Pub Date : 2025-11-01 Epub Date: 2025-08-05 DOI: 10.1016/j.pestbp.2025.106618

Longhui Luo, Lan-Tu Xiong, Xingnan Zhang, Di Zhu, Lian-Hui Zhang, Zi-Ning Cui

{"title":"Quaternary ammonium cationic surfactants target Vfm quorum sensing to suppress the virulence of rice pathogen Dickeya oryzae.","authors":"Longhui Luo, Lan-Tu Xiong, Xingnan Zhang, Di Zhu, Lian-Hui Zhang, Zi-Ning Cui","doi":"10.1016/j.pestbp.2025.106618","DOIUrl":"https://doi.org/10.1016/j.pestbp.2025.106618","url":null,"abstract":"The rice foot rot disease caused by Dickeya oryzae is an important bacterial disease that could cause tremendous economic losses. The virulence factor modulating cluster (Vfm) quorum sensing (QS) system, a major virulence regulatory mechanism conserved in the Dickeya genus, controls the production of zeamines and various extracellular cell wall degradation enzymes in D. oryzae. In this study, we targeted the Vfm QS system of D. oryzae strain EC1 to screen for chemical compounds capable of attenuating its virulence. Using a high-throughput vfmE-lux reporter assay, we screened over 2000 compounds and identified a series of long aliphatic chain compounds - quaternary ammonium cationic surfactants (QACs) that substantially reduced the vfmE promoter activity. Further analysis revealed that QACs blocked strain EC1 response to Vfm signals. Quantitative PCR results showed that the transcriptional expression of the vfm gene cluster and its downstream virulence genes, including those encoding production of phytotoxin zeamines and CWDEs were significantly downregulated. Consistent with their role in blocking Vfm signaling, QACs markedly inhibited the production of virulence determinant zeamines and suppressed the secretion of cellulase and pectinase in strain EC1. Infection assays demonstrated that these compounds substantially reduced the virulence of D. oryzae EC1 toward rice seeds and alleviated the soft rot symptoms in potato tubers. This is the first report that QACs are potent inhibitors of the D. oryzae Vfm QS system, illustrating a promising QS-targeted approach for the control of bacterial rice foot rot disease.","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"214 ","pages":"106618"},"PeriodicalIF":4.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145016031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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