Animals最新文献 - Book学术

Evaluation of the Synergistic Antimicrobial Activity of Essential Oils and Cecropin A Natural Peptide on Gram-Negative Bacteria. 评估精油和天然肽 Cecropin A 对革兰氏阴性菌的协同抗菌活性

IF 2.7 2区农林科学

Animals Pub Date : 2025-01-20 DOI: 10.3390/ani15020282

Filippo Fratini, Chiara Pecorini, Ilaria Resci, Emma Copelotti, Francesca Paola Nocera, Basma Najar, Simone Mancini

{"title":"Evaluation of the Synergistic Antimicrobial Activity of Essential Oils and Cecropin A Natural Peptide on Gram-Negative Bacteria.","authors":"Filippo Fratini, Chiara Pecorini, Ilaria Resci, Emma Copelotti, Francesca Paola Nocera, Basma Najar, Simone Mancini","doi":"10.3390/ani15020282","DOIUrl":"10.3390/ani15020282","url":null,"abstract":"In an era dominated by the phenomenon of antibiotic resistance, it is increasingly important to look for alternatives to synthetic antibiotics. In light of these considerations, the synergistic use of essential oils and Antimicrobial Peptides (AMPs) seems a viable strategy. In this study, we assessed the Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC) and Fractional Inhibitory Concentration (FIC) of three Essential Oils (EOs): winter savory (Satureja montana), bergamot (Citrus bergamia) and cinnamon (Cinnamomum zeylanicum) and of the insect antimicrobial peptide Cecropin A (CecA), alone and in combination with EOs, against two Gram-negative ATCC bacterial strains: Escherichia coli and Salmonella enterica serovar Typhimurium. The MIC results showed that winter savory EO (SmEO) and cinnamon EO (CzEO) exhibited the strongest antibacterial activity against both bacterial strains, whereas bergamot EO (CbEO) and CecA demonstrated comparatively lower antibacterial efficacy. These results were also confirmed by the MBC values. The FIC Indices (FICI) revealed that the most effective synergies were observed with the combinations SmEO/CzEO and SmEO/CbEO against E. coli, while against S. enterica Typhimurium the best combinations were CbEO/CzEO and SmEO/CzEO. Regarding CecA, although it was not the most efficient agent either individually or in combination, it is noteworthy that, when combined, it exhibited antibacterial activity even at a 1:64 dilution.","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11760479/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of Genomic Selection in Beef Cattle Disease Prevention.

IF 2.7 2区农林科学

Animals Pub Date : 2025-01-20 DOI: 10.3390/ani15020277

Ramanathan Kasimanickam, Joao Carlos Pinheiro Ferreira, John Kastelic, Vanmathy Kasimanickam

{"title":"Application of Genomic Selection in Beef Cattle Disease Prevention.","authors":"Ramanathan Kasimanickam, Joao Carlos Pinheiro Ferreira, John Kastelic, Vanmathy Kasimanickam","doi":"10.3390/ani15020277","DOIUrl":"10.3390/ani15020277","url":null,"abstract":"Genomic applications in beef cattle disease prevention have gained traction in recent years, offering new strategies for improving herd health and reducing economic losses in the livestock industry. Advances in genomics, including identification of genetic markers linked to disease resistance, provide powerful tools for early detection, selection, and management of cattle resistant to infectious diseases. By incorporating genomic technologies such as whole-genome sequencing, genotyping, and transcriptomics, researchers can identify specific genetic variants associated with resistance to pathogens like bovine respiratory disease and Johne's disease. These genomic insights allow for more accurate breeding programs aimed at enhancing disease resistance and overall herd resilience. Genomic selection, in particular, enables identification of individuals with superior genetic traits for immune function, reducing the need for antibiotic treatments and improving animal welfare. Moreover, precision medicine, powered by genomic data, supports development of tailored health management strategies, including targeted vaccination plans and antimicrobial stewardship. Incorporation of genomic tools in beef cattle management also offers the potential for early disease detection, facilitating proactive interventions that reduce the spread of infections. Despite challenges like cost, data interpretation and integration into current management systems, the potential advantages of genomic applications in disease prevention are substantial. As these technologies advance, they are anticipated to have crucial roles in improving sustainability (by enhancing herd performance), profitability (by improving overall herd longevity), and biosecurity (by decreasing the likelihood of disease outbreaks) of beef cattle production systems worldwide.","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11759163/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Elevated Lipid Concentrations in Seminal Plasma Can Reduce Sperm Motility in Simmental Bulls.

IF 2.7 2区农林科学

Animals Pub Date : 2025-01-20 DOI: 10.3390/ani15020276

Zhuo Yang, Fang Luo, Chenglei Song, Zhiyuan Ma, Yucheng Tian, Yu Fu, Hao Zheng, Jinzhong Tao

{"title":"Elevated Lipid Concentrations in Seminal Plasma Can Reduce Sperm Motility in Simmental Bulls.","authors":"Zhuo Yang, Fang Luo, Chenglei Song, Zhiyuan Ma, Yucheng Tian, Yu Fu, Hao Zheng, Jinzhong Tao","doi":"10.3390/ani15020276","DOIUrl":"10.3390/ani15020276","url":null,"abstract":"Sperm motility is a key factor influencing male fertility and is associated with metabolic and lipid profiles across species. The aim of this study was to investigate the relationship between sperm motility and the seminal plasma lipid profile in Simmental bulls, and to identify key lipids potentially influencing sperm motility. Semen samples were collected from 26 healthy Simmental bulls with an average age of 4.9 years. Sperm quality was evaluated using computer-assisted sperm analysis (CASA). Based on motility, the samples were divided into two groups: high sperm motility (HSM > 65%) and low sperm motility (LSM < 65%). Compared to the LSM group, the HSM group exhibited significantly higher sperm viability, motility, straight-line velocity, beat-cross frequency, and sperm acrosome integrity, while the sperm malformation rate was lower (p < 0.05). Lipid profiles were determined using LC-MS/MS, and 40 differential lipids were identified by multivariate statistical analysis. Among them, 39 lipids were upregulated in the LSM group compared to the HSM group. They were primarily triglycerides and carnitines, mainly involved in four metabolic pathways related to glycerophospholipid and linoleic acid metabolism. Notably, PC (16:0/20:4; 14:0/18:3), LPC (22:4/0:0; 22:6/0:0), and PE (14:0/18:1; 18:1/20:3) were diagnosed with great accuracy (AUC > 0.7), which means they may serve as potential biomarkers for sperm motility.","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11762868/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Estimation of Optimal and Maximum Standardized Ileal Digestible Methionine Requirements Based on Performance for Male Broilers Aged 0 to 21 Days.

IF 2.7 2区农林科学

Animals Pub Date : 2025-01-20 DOI: 10.3390/ani15020278

Su-Hyun An, Changsu Kong

{"title":"Estimation of Optimal and Maximum Standardized Ileal Digestible Methionine Requirements Based on Performance for Male Broilers Aged 0 to 21 Days.","authors":"Su-Hyun An, Changsu Kong","doi":"10.3390/ani15020278","DOIUrl":"10.3390/ani15020278","url":null,"abstract":"Two experiments were conducted with Ross 308 male broiler chickens to determine the standardized ileal digestible (SID) methionine (Met) requirement for maximum weight gain during the starter (0 to 10 days) and grower (10 to 21 days) phases. A total of 720 and 288 birds were allocated to Experiments 1 and 2, respectively, and assigned six dietary treatments in a randomized complete block design. The experimental diets were formulated with corn, soybean meal, and synthetic amino acids to achieve varying SID Met levels (3.8 to 5.8 g/kg in experiment 1 and 3.0 to 5.5 g/kg in experiment 2), with increments of 0.4 and 0.5 g/kg. Weight gain, feed intake, and the gain-to-feed ratio (G:F) increased quadratically (p < 0.05) as dietary SID Met increased. The SID Met requirements for maximum weight gain and G:F in the starter phase were 5.1 g/kg (79.5 mg/d) and 5.3 g/kg (89.8 mg/d), while in the grower phase, they were 5.5 g/kg (343.0 mg/d) and 5.4 g/kg (330.3 mg/d), respectively. These findings, divided into two growth phases, provide guidelines to facilitate the optimal and maximum performance of young male broilers, and this may facilitate the selection of appropriate values for formulating broiler feed. These findings also highlight the importance of Met supplementation strategies for improving performance.","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11759192/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advanced Imaging for Congenital Left Ventricular Diverticulum in a Dog: The Role of Electrocardiosynchronous CT.

IF 2.7 2区农林科学

Animals Pub Date : 2025-01-20 DOI: 10.3390/ani15020280

Miki Hirose, Lina Hamabe, Kazumi Shimada, Aki Takeuchi, Kazuyuki Terai, Aimi Yokoi, Ahmed Farag, Akari Hatanaka, Rio Hayashi, Katsuhiro Matsuura, Ryou Tanaka

引用次数: 0

Tumor-Infiltrating Lymphocytes and Neutrophils and Their Location in Canine Mammary Neoplasms with a Solid Arrangement: A Prognostic Factor? 实性排列的犬乳腺肿瘤中的肿瘤浸润淋巴细胞和中性粒细胞及其位置：预后因素？

IF 2.7 2区农林科学

Animals Pub Date : 2025-01-20 DOI: 10.3390/ani15020287

Mayra C Flecher, Marina P Reys, Débora Balabram, Karen Y R Nakagaki, Geovanni D Cassali

{"title":"Tumor-Infiltrating Lymphocytes and Neutrophils and Their Location in Canine Mammary Neoplasms with a Solid Arrangement: A Prognostic Factor?","authors":"Mayra C Flecher, Marina P Reys, Débora Balabram, Karen Y R Nakagaki, Geovanni D Cassali","doi":"10.3390/ani15020287","DOIUrl":"10.3390/ani15020287","url":null,"abstract":"In canine mammary neoplasms, greater inflammation is associated with higher histological grade, lymphatic invasion, and metastases. This retrospective study assessed the density of peri- and intratumoral tumor-infiltrating lymphocytes (TILs), tumor-associated neutrophils (TANs), and CD3+ and CD79+ lymphocytes in canine mammary neoplasms with a solid arrangement, and associated such data with histological types, immunophenotype, prognostic factors, cyclooxygenase-2 (Cox-2) expression and overall and cancer-specific survival. Sixty-one neoplasms with a solid arrangement were classified as malignant myoepitheliomas (6/9.8%), solid papillary carcinomas (8/13.1%), carcinomas with a solid pattern (9/14.8%), basaloid carcinomas (BC) (19/31.1%), and malignant adenomyoepitheliomas (19/31.1%). Intra- and peritumoral TILs, TANs, and TCD3+ and BCD79+ lymphocytes were counted, and based on the resulting median, the neoplasms were divided into low or high cell infiltration. BCs had the lowest density of intratumoral TILs (p = 0.02), and luminal B neoplasms showed a significantly higher density of intratumoral TCD3+ than luminal A cases. Neoplasms with a higher density of peritumoral CD3+ and CD79+ had significantly greater proliferative activity. High infiltration of intratumoral BCD79+ lymphocytes was related to nodal metastasis (p = 0.03). Intratumoral TILs and TCD3+ were associated with shorter survival time. Therefore, intratumoral lymphocyte infiltration is possibly an important feature in the progression of cancer and influences the survival in bitches with solid arrangement neoplasms.","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11762997/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prepartum Dietary Supplementation of Potassium Humate Improves Postpartum Lactation Performance, Metabolic Profile of Multiparous Cows, and Immune Response of Their Calves. 产前膳食中添加腐植酸钾可提高多胎母牛的产后泌乳性能、新陈代谢状况及其犊牛的免疫反应。

IF 2.7 2区农林科学

Animals Pub Date : 2025-01-20 DOI: 10.3390/ani15020279

Cangir Uyarlar, Abdur Rahman, Eyup Eren Gultepe, İbrahim Sadi Cetingul, Muhammad Uzair Akhtar, Ismail Bayram

{"title":"Prepartum Dietary Supplementation of Potassium Humate Improves Postpartum Lactation Performance, Metabolic Profile of Multiparous Cows, and Immune Response of Their Calves.","authors":"Cangir Uyarlar, Abdur Rahman, Eyup Eren Gultepe, İbrahim Sadi Cetingul, Muhammad Uzair Akhtar, Ismail Bayram","doi":"10.3390/ani15020279","DOIUrl":"10.3390/ani15020279","url":null,"abstract":"This research was carried out to determine the effects of potassium humate on the lactation performance and metabolic parameters of dairy cows during the transition period. Potassium humate was added to the concentrate feed at the following levels: (a) control (0%), (b) 0.5%, (c) 1%, (d) 1.5%, and (e) 2% humas, during the dry period from -60 to 0 days until calving. The results indicated that the total milk yield after 305 days was higher in the 0.5% group than in the 2% humic acid group. The average daily milk yield from lactation was also greater in the 0.5% group than in the 2% humic acid group. In terms of metabolic health and blood biochemistry, lymphocytes, neutrophils, monocytes, NEFAs, and BHBA were different among the treatment groups. No effects were detected on the blood physiology parameters of the calves. The IgG concentration in the colostrum and serum of calves on day 1 and 2 were higher in the 0.5% and 1% humic acid groups, respectively, than in the other groups. Overall, adding humic acid, especially at the dose of 0.5%, to the concentrate feed of dairy cows during the dry period resulted in an increased postpartum milk yield for the cows and increased serum IgG in both the cows and calves, with decreased NEFAs on the calving day and decreased postpartum BHBA for cows.","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11762799/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Sublingua of Lemur catta and Varecia variegata: Only a Cleaning Function?

IF 2.7 2区农林科学

Animals Pub Date : 2025-01-20 DOI: 10.3390/ani15020275

Juan Francisco Pastor, Magdalena Natalia Muchlinski, Josep María Potau, Aroa Casado, Yolanda García-Mesa, José Antonio Vega, Roberto Cabo

{"title":"The Sublingua of Lemur catta and Varecia variegata: Only a Cleaning Function?","authors":"Juan Francisco Pastor, Magdalena Natalia Muchlinski, Josep María Potau, Aroa Casado, Yolanda García-Mesa, José Antonio Vega, Roberto Cabo","doi":"10.3390/ani15020275","DOIUrl":"10.3390/ani15020275","url":null,"abstract":"The sublingua is an anatomical structure located under the tongue. This rare organ can be present in some animals as a rudimentary structure, but among prosimian primates, such as lemurs and lorises, it is fully developed. In addition to the sublingua, prosimians have modified lower incisors and canines called \"dental comb\". The anatomy of sublingua has been studied macro and microanatomically since the early 19th century. Most authors argue that the sublingua is an oral morphological adaptation to develop a toothbrush's role in cleaning the dental comb. However, others assert that the functional role has yet to be established. Comparative studies of macro and microanatomy are scarce or incomplete for primates; thus, the putative function remains unclear. To better understand the functional significance of the sublingua, we studied this structure in Lemur catta and Varecia variegata specimens using histochemical staining techniques and scanning electron microscopy with microanalysis. The new data obtained provide a fuller picture of the role assigned to sublingua so far, which could be more complex. In light of the morphological findings, we should consider additional roles/functions of the sublingua, including but not limited to food processing, grooming or social behavior.","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11760434/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunohistochemical Expression of Vascular Endothelial Growth Factor (VEGF) in Primary Canine Mast Cell Tumors and Related Regional Lymph Node Metastasis.

IF 2.7 2区农林科学

Animals Pub Date : 2025-01-20 DOI: 10.3390/ani15020283

Alice Corrêa Rassele, Isabella Oliveira Almeida, Maylla Garschagen Gava, Pedro Antônio Bronhara Pimentel, Antonio Giuliano, Felipe Augusto Ruiz Sueiro, Ayisa Rodrigues de Oliveira, Andrigo Barboza de Nardi, Rodrigo Dos Santos Horta

{"title":"Immunohistochemical Expression of Vascular Endothelial Growth Factor (VEGF) in Primary Canine Mast Cell Tumors and Related Regional Lymph Node Metastasis.","authors":"Alice Corrêa Rassele, Isabella Oliveira Almeida, Maylla Garschagen Gava, Pedro Antônio Bronhara Pimentel, Antonio Giuliano, Felipe Augusto Ruiz Sueiro, Ayisa Rodrigues de Oliveira, Andrigo Barboza de Nardi, Rodrigo Dos Santos Horta","doi":"10.3390/ani15020283","DOIUrl":"10.3390/ani15020283","url":null,"abstract":"Vascular endothelial growth factor (VEGF) is an important regulator of angiogenesis and has been identified as an autocrine growth factor for neoplastic cells of several tumors. The aim of this study was to evaluate VEGF expression in canine mast cell tumors and their respective lymph node metastases. For this purpose, 28 patients with mast cell tumors and confirmed regional lymph node metastasis were selected. The samples were submitted for analysis with immunohistochemistry. VEGF was expressed in 14/28 patients (50%) and 35.7% of primary MCTs (10/28), and only 14.3% expressed VEGF in both the primary tumor and its respective metastasis (4/28), with fair agreement (Κ = 0.250). There was a weak correlation between VEGF and tumor size (p = 0.016, rs = 0.045). In this cohort, survival time was correlated with Kiupel grade, mitotic index, tumor necrosis, tumor location, and systemic treatment. VEGF immunolabeling had no influence on survival; however, patients with positive expression may benefit from specific therapy.","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11761284/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143036069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a Portable Rapid Detection Method for Porcine Epidemic Diarrhea Virus Using Reverse Transcription-Recombinase-Aided Amplification Technology.

IF 2.7 2区农林科学

Animals Pub Date : 2025-01-20 DOI: 10.3390/ani15020281

Yiran Zhao, Weijie Yi, Qicheng Yang, Jiahao Li, Yanke Shan, Fei Liu

{"title":"Development of a Portable Rapid Detection Method for Porcine Epidemic Diarrhea Virus Using Reverse Transcription-Recombinase-Aided Amplification Technology.","authors":"Yiran Zhao, Weijie Yi, Qicheng Yang, Jiahao Li, Yanke Shan, Fei Liu","doi":"10.3390/ani15020281","DOIUrl":"10.3390/ani15020281","url":null,"abstract":"Porcine epidemic diarrhea virus (PEDV) continues to spread globally, causing clinical symptoms in piglets, including watery diarrhea, vomiting, and dehydration. Its exceptionally high morbidity and mortality rate contributes significantly to the economic losses of the swine industry. The continuous genetic mutations of PEDV have compromised the effectiveness of classical strain vaccines. Early detection and accurate diagnosis are therefore crucial for controlling its further spread. Developing a detection method that is user-friendly, highly sensitive, and efficient is crucial for disease control. In this study, a point-of-care rapid detection method for PEDV was successfully established using reverse transcription-recombinase-aided amplification (RT-RAA) technology. This method enables results to be obtained within 20 min of amplification at a constant temperature of 42 °C. It demonstrates high sensitivity, with a detection limit as low as 1 copy/μL, and shows strong specificity, with no cross-reactivity observed with seven other common swine pathogens. When applied to clinical samples, the results were 100% consistent with those obtained by RT-qPCR. This method is distinguished by its portable instrumentation and simple operation, making it particularly suitable for resource-constrained settings.","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11761192/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143036010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0