Animals最新文献

{"title":"Advances in the Diagnosis of Reproductive Disorders in Male Camelids.","authors":"Abdelmalek Sghiri, Muhammad Salman Waqas, Michela Ciccarelli, Abelhaq Anouassi, Ahmed Tibary","doi":"10.3390/ani15192931","DOIUrl":"10.3390/ani15192931","url":null,"abstract":"<p><p>Male fertility is important to ensure herd health and productivity. The camelid male breeding soundness examination (BSE) is strongly recommended because natural mating remains the primary breeding method due to the challenges in semen cryopreservation and artificial insemination. Guidelines for the BSE have been proposed but not adopted in practice. The investigation of male reproductive failure includes history, general health examination, examination of the genitalia, semen evaluation, and testing for contagious diseases. Difficulties in ejaculate collection and semen viscosity are challenges in camelid male fertility investigation. This review summarizes the outcomes of BSE in our practice on South American camelids (SACs) and camels. The results and discussion are presented under four main categories: congenital defects, <i>impotentia coeundi, impotentia generandi</i>, and male reproductive emergencies. There is a difference between camels and SACs in the incidence of various disorders. Congenital defects are common in SACs in particular cryptorchidism, testicular hypoplasia and rete testis cysts. Orchitis is more common in camels, particularly in areas where brucellosis is prevalent. Testicular degeneration occurs in all camelids and has been associated with heat stress, aging, systemic diseases and overuse of anabolic steroids. Precise diagnosis of fertility impairment may require disease testing, testicular biopsy, cytogenetics and endocrine evaluation. A significant proportion of males are referred because of reproductive emergencies, due to poor management, which results in loss of genetic potential. Implementation of regular BSE is possible in SACs but can be difficult in dromedaries because of the large variation in breed characteristics and management systems.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 19","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12523255/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145297965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive Identification of the Bovine KLF Gene Family and Its Functional Regulation in Muscle Development: Insights from Single-Nuclei Transcriptomics.","authors":"Fengying Ma, Le Zhou, Lili Guo, Chencheng Chang, Dan Dan, Yanchun Bao, Guiting Han, Mingjuan Gu, Lin Zhu, Risu Na, Caixia Shi, Jiaxin Zhang, Wenguang Zhang","doi":"10.3390/ani15192930","DOIUrl":"10.3390/ani15192930","url":null,"abstract":"<p><p>The Krüppel-like factor (<i>KLF</i>) family of transcription regulators plays pivotal roles in adipogenesis, myogenesis, and metabolism. While comprehensively studied in humans and mice, its characterization in cattle remains limited, especially within the skeletal muscle niche. This study aimed to systematically characterize the <i>KLF</i> family in Bos taurus and elucidate its role in breed-specific muscular development. We employed an integrated approach of comparative genomics and single-nucleus RNA sequencing (snRNA-seq) on longissimus dorsi muscle from Angus (ANG, beef breed) and Holstein (HST, dairy breed) cattle. Phylogenomic analysis identified 14 <i>KLF</i> genes, revealing evolutionary conservation and potential functional divergence. snRNA-seq delineated 11 distinct cell populations and uncovered cell-type-specific expression patterns of <i>KLFs</i>. Further machine learning based analysis pinpointed <i>KLF6</i>, <i>KLF9</i>, <i>KLF10</i>, and <i>KLF12</i> as key global drivers of transcriptional differences between breeds, while <i>KLF6</i> was identified as a major cell-type-specific contributor in lymphatic endothelial cells. Our work provides a foundational resource for understanding the <i>KLF</i> family in cattle and identifies promising candidate genes for improving meat production traits through molecular breeding.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 19","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12524274/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145298116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Monitoring Night-Time Activity Patterns of Laying Hens in Response to Poultry Red Mite Infestations Using Night-Vision Cameras.","authors":"Sam Willems, Hanne Nijs, Nathalie Sleeckx, Tomas Norton","doi":"10.3390/ani15192928","DOIUrl":"10.3390/ani15192928","url":null,"abstract":"<p><p>The poultry red mite (PRM) feeds on hens' blood at night, disrupting sleep, harming welfare, and reducing productivity. Effective control may lie in dynamic Integrated Pest Management (IPM), which relies on routine monitoring and adaptation to farm conditions. This study investigated how PRM infestations affect the night-time activity of hens. Three groups of eight hens, housed in enriched cages, were monitored with night-vision cameras over a two-month period, both before and after artificial PRM introduction, while PRM levels were simultaneously recorded. To quantify changes in behaviour, we developed an activity-monitoring algorithm that extracts both group-level and individual night-time activity patterns from video recordings. Group activity between 18:00 and 03:00 was analyzed hourly, and individual activity between 21:00 and 00:00 was classified into four activity categories. Before infestation, group activity declined after 19:00, remained low from 20:00 to 01:00, and peaked just before the end of the dark period. After infestation, activity remained elevated with no anticipatory activity peak towards the end of the dark period. Individual data showed an increase in time spent in the most active activity category from 24% to 67% after infestation. The rise in calculated activity was supported by a nearly 23-fold increase in annotated PRM-related behaviours, specifically head shaking and head scratching. These findings suggest that PRM mostly disrupted sleep from two hours after lights-off to two hours before lights-on and may have acted as a chronic stressor. Automated video-based monitoring could strengthen dynamic IPM in commercial systems.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 19","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12524107/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145298331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histological and Proteomic Approaches to Assessing the Adrenal Stress Response in Common Dolphins (<i>Delphinus delphis</i>).","authors":"Claudia Medina Santana, Orla Slattery, Jim O'Donovan, Sinéad Murphy","doi":"10.3390/ani15192924","DOIUrl":"10.3390/ani15192924","url":null,"abstract":"<p><p>The adrenal glands are central to the stress response in cetaceans, yet their morphological and molecular changes under chronic stress remain poorly described. We investigated adrenal histology and protein composition in stranded common dolphins (<i>Delphinus delphis</i>) to assess whether post-mortem material can provide insights into stress physiology. Adrenal glands from 58 dolphins recovered along the Irish coast during a period of reported nutritional stress in the species were analyzed for adrenal mass, cortex-to-medulla (C:M) ratios, and cortical cell density. Additionally, two archival formalin-fixed, paraffin-embedded (FFPE) tissues were included in a pilot trial to assess the feasibility of protein extraction and mass spectrometry analysis. While adrenal mass did not differ significantly between stress types, chronically stressed dolphins exhibited significantly higher C:M ratios and cortical mass, consistent with cortical hypertrophy. Protein extraction from FFPE tissues was feasible, with the in-gel digestion method yielding more proteins (136) than the filter-aided sample preparation method (22). These findings demonstrate that histological and proteomic approaches can detect stress-related signatures in dolphins and highlight the potential of archival tissues for retrospective biomarker discovery.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 19","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12523369/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145298154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbiota-Metabolite-Host Crosstalk Mediates the Impact of Dietary Energy Levels on Colonic Homeostasis in High-Altitude Ruminants.","authors":"Qinran Yu, Ning Li, Pengjia Bao, Chun Huang, Qingbo Zheng, Tong Wang, Chaofan Ma, Jingying Deng, Fengtao Jiang, Jianlei Jia, Ping Yan","doi":"10.3390/ani15192929","DOIUrl":"10.3390/ani15192929","url":null,"abstract":"<p><p>The colon plays a crucial role in energy metabolism and intestinal health of ruminants during various physiological stages. Plateau ruminants have long been subjected to extreme environments characterized by hypoxia, cold, and nutritional scarcity, which makes their dependence on energy metabolism particularly pronounced. However, existing research on the regulatory effects of dietary energy levels on the colonic function of plateau ruminants is still quite limited. This study involved 60 healthy male Pamir yaks with consistent body conditions, which were randomly divided into three groups: a low-energy diet group (YG, Neg 1.53 MJ/kg), a medium-energy diet group (QG, Neg 2.12 MJ/kg), and a high-energy diet group (RG, Neg 2.69 MJ/kg). Each yak was provided with 5 kg of mixed feed daily over a 170-day feeding trial. The results indicated that a high-energy diet enhanced growth performance in yaks (<i>p</i> < 0.05). However, it also induced local colonic inflammation, decreased levels of immune factors (IgA, IgG, and IL-10), and increased the abundance of potentially pathogenic bacteria, such as <i>Klebsiella</i> and <i>Campylobacter</i> (<i>p</i> < 0.05). Conversely, a medium-energy diet fostered the proliferation of beneficial bacteria such as <i>Bradymonadales</i>, <i>Parabacteroides</i>, and <i>Mogibacterium</i> (<i>p</i> < 0.05), and preserved immune homeostasis. Additionally, multi-omics analysis revealed that the QG group was significantly enriched in key metabolic pathways, including pyruvate metabolism and glycine, serine, and threonine metabolism and panto-thenate and CoA biosynthesis pathways, among others (<i>p</i> < 0.05), demonstrating a synergistic regulatory effect among the microbiome, metabolism, and host. In summary, a moderate-energy diet can promote the proliferation of beneficial bacteria in the extreme environment of the plateau. By regulating pathways such as Amino acid, Nucleotide, and Lipid metabolism, it coordinates the expression of key host genes and metabolite levels, effectively balancing immune signals and energy metabolism. This interaction establishes a beneficial microbial-metabolism-host pattern that supports colon health.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 19","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12524189/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145298170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cryptorchidism in Equid: A Retrospective Study on Diagnostic Approach, Anaesthesia Management, Surgical Treatment and Outcomes.","authors":"Irene Nocera, Rebecca Moroni, Diana Fanelli, Alessandra Rota, Chiara Di Franco, Camilla Ungari, Caterina Puccinelli, Marco Gandini, Gessica Giusto, Iacopo Vannozzi","doi":"10.3390/ani15192923","DOIUrl":"10.3390/ani15192923","url":null,"abstract":"<p><p>Over the years, various diagnostic and surgical techniques have been developed to recognise and treat cryptorchidism in equid. This study retrospectively analysed cases of cryptorchidism referred to two centres between 2012 and 2025. Clinical data collected included species, breed, age, gender, location of retained testis, diagnostic approach, anaesthesia plan and surgical treatment, perioperative medical treatment, intra-operative complications and outcomes. A total of 37 clinical cases were included, 1/37 was a donkey and 1/37 was an intersex female. Most of the horses were three years old and belonged to western riding horse breeds. Ultrasound examination correctly identified the location of the retained testis in 87% of cases. The anaesthesia plan includes a local anaesthesia block, mainly a local injection for the infiltration of the retained testis. The laparoscopic standing flank technique was the main approach, performed in 82% of cases. Complications were encountered in 9% of cases, and the median discharge time was 2 days. In our study, Quarter Horse-type horses were overrepresented, and three horses were sons of the same Quarter Horse stallion, suggesting a genetic component in aetiology. The use of ultrasound might confirm cryptorchidism in both abdominal and inguinal locations. Standing flank laparoscopy was the most frequently chosen procedure, over recumbent laparoscopy and inguinal open orchiectomy. The outcome reported a low rate of intra-operative complications.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 19","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12523330/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145298151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficiency of Recurrent Genomic Selection in Panmictic Populations.","authors":"José Marcelo Soriano Viana, Jean Paulo Aparecido da Silva, Paulo Sávio Lopes","doi":"10.3390/ani15192925","DOIUrl":"10.3390/ani15192925","url":null,"abstract":"<p><p>Simulation-based studies can support breeders' decisions inexpensively, since there is no need to perform a new procedure. The objective was to assess the efficiency of recurrent genomic selection in panmictic population under additive-dominance and additive-dominance with epistasis models. We assumed two broiler chicken populations with contrasting linkage disequilibrium (LD) levels, 38,500 SNPs, and 1000 genes controlling feed conversion ratio. We applied recurrent genomic selection over seven cycles. The genomic selection efficacy, expressed as realized total genetic gain, was proportional to the LD level and genotypic variance. Genomic selection required model updating to achieve a higher efficacy. The training set size required by genomic selection can be as low as 10%/generation. Under this low-cost scenario, the genomic selection efficacy was slightly lower than the maximum efficacy. There is no difference between genetic evaluation methods regarding the decrease in the genotypic variance due to selection. In general, additive value prediction accuracies and realized genetic gains were highly correlated. The accumulated inbreeding level was not high due to avoidance of sib cross. The genomic inbreeding coefficient over generations was close to zero. Except for dominant epistasis, the efficacy of genomic selection was 4.1 to 46.2% lower than the efficacy under no epistasis.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 19","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12523783/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145298263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Parenteral Copper and Zinc Administration on Reproduction, Inflammation, and Antioxidant Responses of <i>Bos indicus</i> Beef Heifers.","authors":"Luana Gomes da Silva, Marcelo Vedovatto, Juliana Ranches, Edilane Costa Martins, Matheus Fellipe Ferreira, Eduardo de Assis Lima, Luiz Carlos Louzada Ferreira, Willian Vaniel Alves Dos Reis, Gumercindo Loriano Franco","doi":"10.3390/ani15192926","DOIUrl":"10.3390/ani15192926","url":null,"abstract":"<p><p>Two experiments evaluated the effects of copper (Cu) and zinc (Zn) injection on body weight (BW), body condition score (BCS), pregnancy rate, ovarian traits, and antioxidant and inflammatory responses of beef heifers. In Exp. 1, 29 heifers were assigned to having saline or CuZn (a solution containing 15 and 50 mg/mL of Cu and Zn) subcutaneously injected (5 mL/heifer) 9 days before artificial insemination. Exp. 2 was conducted to increase the statistical power to evaluate pregnancy rate, and 283 heifers were assigned to either the saline or CuZn group. In Exp. 1, CuZn heifers tended (<i>p</i> ≤ 0.10) to gain more BW and to have greater corpus luteum size and plasma concentration of glutathione peroxidase. No effects of treatment were detected (<i>p</i> ≥ 0.18) for BCS; estrus score; serum concentration of Cu, Zn, and cortisol; and plasma concentration of haptoglobin, ceruloplasmin, and superoxide dismutase. In Exp. 2, CuZn heifers had greater (<i>p</i> < 0.01) BW and serum Cu. The CuZn heifers with low BCS had greater (<i>p</i> ≤ 0.05) estrus scores and tended (<i>p</i> = 0.10) to have greater pregnancy rates. Thus, injecting CuZn may be an effective strategy to enhance growth, reproductive performance, and antioxidant responses in heifers, especially when their BCS is below 5.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 19","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12523923/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145298100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"No Effect of a Commercially Used Odor Repellent for Roe Deer (<i>Capreolus capreolus</i>) Protection During Meadow Harvest.","authors":"Jan Cukor, Klára Matějka Košinová, Rostislav Linda, Vlastimil Skoták, Richard Ševčík, Tereza Červená, Kateřina Brynychová, Zdeněk Vacek","doi":"10.3390/ani15192932","DOIUrl":"10.3390/ani15192932","url":null,"abstract":"<p><p>In Central Europe, the fawning season of roe deer (<i>Capreolus capreolus</i>) directly overlaps with meadow and alfalfa harvest, typically from late May to early June. During these operations, tens or more likely hundreds of thousands of fawns are mutilated by agricultural machinery. To mitigate this unethical mortality, wildlife managers often deploy odor repellents to drive roe deer individuals from high-risk fields before mowing. Therefore, we evaluated repellent efficacy in a paired design. The abundance of roe deer was quantified by drones equipped with thermal cameras before and after repellent application and then compared with untreated control meadows. Results showed high adult abundance that did not differ significantly among treatments. The highest median was paradoxically observed on meadows \"after application\" (8.25 ind./10 ha), followed by \"not treated\" meadows (7.92 ind./10 ha), and \"before application\" (5.72 ind./10 ha). For fawns, differences between treated and untreated plots were likewise non-significant. Their numbers increased over time after application, consistent with the peak of parturition in the second half of May. Overall, the study confirms that the tested odor repellent, when applied according to the manufacturer's protocol, did not reduce roe deer presence on meadows. This underscores the need to consider alternative approaches, such as the use of thermal-imaging drones combined with the subsequent translocation of detected fawns to safe locations.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 19","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12523241/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145298282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EnhancedMulti-Scenario Pig Behavior Recognition Based on YOLOv8n.","authors":"Panqi Pu, Junge Wang, Geqi Yan, Hongchao Jiao, Hao Li, Hai Lin","doi":"10.3390/ani15192927","DOIUrl":"10.3390/ani15192927","url":null,"abstract":"<p><p>Advances in smart animal husbandry necessitate efficient pig behavior monitoring, yet traditional approaches suffer from operational inefficiency and animal stress. We address these limitations through a lightweight YOLOv8n architecture enhanced with SPD-Conv for feature preservation during downsampling, LSKBlock attention for contextual feature fusion, and a dedicated small-target detection head. Experimental validation demonstrates superior performance: the optimized model achieves a 92.4% mean average precision (mAP@0.5) and 87.4% recall, significantly outperforming baseline YOLOv8n by 3.7% in AP while maintaining minimal parameter growth (3.34M). Controlled illumination tests confirm enhanced robustness under strong and warm lighting conditions, with performance gains of 1.5% and 0.7% in AP, respectively. This high-precision framework enables real-time recognition of standing, prone lying, lateral lying, and feeding behaviors in commercial piggeries, supporting early health anomaly detection through non-invasive monitoring.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 19","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12523645/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145298230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}