Animals最新文献 - Book学术

Correction: Zheng et al. Effects of Bacillus coagulans on Growth Performance, Digestive Enzyme Activity, and Intestinal Microbiota of the Juvenile Fourfinger Threadfin (Eleutheronema tetradactylum). Animals 2025, 15, 515. 更正：郑等人。凝固芽孢杆菌对四指丝虫鱼幼鱼生长性能、消化酶活性和肠道菌群的影响动物2025,15,515。

IF 3.2 2区农林科学

Animals Pub Date : 2026-10-28 DOI: 10.3390/ani16213402

Anna Zheng, Jiaqin Hu, Evodia Moses Mkulo, Minxuan Jin, Linjuan Wang, Huijuan Zhang, Baogui Tang, Hui Zhou, Bei Wang, Jiansheng Huang, Zhongliang Wang

引用次数: 0

Correction: Huang et al. The Impact of Mutant EDNRB on the Two-End Black Coat Color Phenotype in Chinese Local Pigs. Animals 2025, 15, 478. 更正：Huang et al.。EDNRB突变体对中国地方猪两头黑毛色表型的影响。动物2025,15,478。

IF 3.2 2区农林科学

Animals Pub Date : 2026-10-26 DOI: 10.3390/ani16213370

Min Huang, Zuohao Wen, Tao Huang, Xiaolong Zhou, Zhijun Wang, Songbai Yang, Ayong Zhao

引用次数: 0

Genome-Wide Detection of Genetic Diversity and Genomic Structure in Hanwoo Cows. 韩宇奶牛遗传多样性和基因组结构的全基因组检测。

IF 3.2 2区农林科学

Animals Pub Date : 2026-10-21 DOI: 10.3390/ani16203320

Seungwon Oh, Duhak Yoon

{"title":"Genome-Wide Detection of Genetic Diversity and Genomic Structure in Hanwoo Cows.","authors":"Seungwon Oh, Duhak Yoon","doi":"10.3390/ani16203320","DOIUrl":"10.3390/ani16203320","url":null,"abstract":"This study aimed to utilize genomic information to elucidate the genetic diversity and structure of Hanwoo cows and develop a more efficient improvement system. Genomic DNA was extracted from hair root samples of 7273 Hanwoo cows, and genotypes were determined using a customized Illumina Hanwoo SNP50k BeadChip. A total of 41,884 single-nucleotide polymorphisms (SNPs) covering approximately 2500 Mb of the genome were selected for analysis, along with 7166 Hanwoo cows that passed quality control. The mean observed and expected heterozygosity were 0.358 ± 0.125 and 0.357 ± 0.124, respectively. A total of 94,560 runs of homozygosity (ROHs) were observed, with an average of 13.2 per animal. Linkage disequilibrium (LD) was estimated using two parameters, D' and r2, which were found to be 0.381 ± 0.309 and 0.054 ± 0.126, respectively, on autosomes. LD declined sharply from< 50 kb to 50-100 kb. In addition, 1818 haplotype blocks were identified on autosomes, totaling 88.27 Mb (3.53% of the autosomal length). The most common haplotype block size was two SNPs, with 1101 blocks. Finally, the effective population size (Ne) was 2853 at 1691 generations but decreased to 277 at 13 generations. These results provide genomic information for further studies and will be helpful for Hanwoo breeding programs.","PeriodicalId":7955,"journal":{"name":"Animals","volume":"16 20","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13700702/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149260909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression Profile of Transfer RNA-Derived Fragments in Chinese Mitten Crab (Eriocheir sinensis) After Challenge with Aeromonas hydrophila. 嗜水气单胞菌侵染中华绒螯蟹后转移rna衍生片段的表达谱

IF 3.2 2区农林科学

Animals Pub Date : 2026-10-21 DOI: 10.3390/ani16203312

Tongtong Kong, Lei Liu, Hanyu Liu, Ngoc Tuan Tran

{"title":"Expression Profile of Transfer RNA-Derived Fragments in Chinese Mitten Crab (Eriocheir sinensis) After Challenge with Aeromonas hydrophila.","authors":"Tongtong Kong, Lei Liu, Hanyu Liu, Ngoc Tuan Tran","doi":"10.3390/ani16203312","DOIUrl":"10.3390/ani16203312","url":null,"abstract":"The Chinese mitten crab (Eriocheir sinensis) is an important cultured crab in China; however, its aquaculture productivity is severely limited by bacterial pathogens, including Aeromonas hydrophila. Transfer RNA-derived fragments (tRFs) are an emerging class of functional small non-coding RNAs that have been implicated in host-pathogen interactions and immune regulation; yet, their roles in the antibacterial defense of crustaceans remain poorly understood. To investigate the immune response of the Chinese mitten crab to A. hydrophila infection, we employed high-throughput sequencing to characterize the expression profiles and functional features of tRFs in crab hemocytes following a bacterial challenge. The results revealed that tRFs exhibited a conserved length distribution (15-20 nt) and subtype composition, with 5'-tRFs representing the dominant subtype in both control and infected groups. Notably, infection specifically induced the abundance of tRF fragments of 35-nt tRF fragments and slightly altered the proportion of tRF subtypes. Differential expression analysis identified 415 significantly upregulated and 3 downregulated tRFs upon A. hydrophila infection, suggesting widespread changes in tRF abundance during infection. Target prediction and functional enrichment analyses were performed for the top 30 differentially expressed tRFs; the predicted targets were significantly associated with VEGF signaling pathway, DNA replication proteins, FoxO signaling pathway, and NF-kappa B signaling pathway. The reliability of the sequencing data was confirmed by stem-loop RT-qPCR validation. Collectively, our findings advance the understanding of tRF expression in crustacean immunity and suggest that tRFs may be associated with the immune response of crabs against bacterial infection, pending functional validation.","PeriodicalId":7955,"journal":{"name":"Animals","volume":"16 20","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13700734/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149260833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coronavirus Diversity in an Italian Bat Population Sampled During the Pandemic in 2020. 在2020年大流行期间取样的意大利蝙蝠种群中的冠状病毒多样性。

IF 3.2 2区农林科学

Animals Pub Date : 2026-10-21 DOI: 10.3390/ani16203319

Gabriele Vaccari, Ilaria Di Bartolo, Luca Dorigo, Angelo Leandro Dreon, Stefano Toppo, Margherita Morpurgo, Edoardo Vernier, Luigi Bertolotti, Andrea Crisanti, Maurizio Guido Paoletti, Enrico Lavezzo, Luca De Sabato

{"title":"Coronavirus Diversity in an Italian Bat Population Sampled During the Pandemic in 2020.","authors":"Gabriele Vaccari, Ilaria Di Bartolo, Luca Dorigo, Angelo Leandro Dreon, Stefano Toppo, Margherita Morpurgo, Edoardo Vernier, Luigi Bertolotti, Andrea Crisanti, Maurizio Guido Paoletti, Enrico Lavezzo, Luca De Sabato","doi":"10.3390/ani16203319","DOIUrl":"10.3390/ani16203319","url":null,"abstract":"Evolutionary studies have established a correlation between human and bat coronaviruses (CoV), identifying bats as the natural reservoir for Coronaviridae. Over the past two decades, three major zoonotic spillover events have been described: Severe Acute Respiratory Syndrome CoV (SARS-CoV) and Middle East Respiratory Syndrome CoV (MERS-CoV), which caused significant global outbreaks, and SARS-CoV-2, which triggered the COVID-19 pandemic. Despite these events, the true diversity of coronavirus species harbored by bats remains largely unmapped, even as the discovery of novel strains continues to accelerate. In this study, we analyzed 37 fecal swab samples collected in Northern Italy, targeting the bat genera Miniopterus, Myotis, and Rhinolophus. Coronaviruses were detected using a pan-coronavirus (Pan-CoV) nested RT-PCR in five bat samples: three tested positive for Alphacoronaviruses (Alpha-CoVs) and two for Betacoronaviruses (Beta-CoVs). Positive samples were subjected to Pan-CoV Illumina next-generation sequencing (NGS), and two samples yielded sufficient read depth for robust contig assembly. Phylogenetic analysis revealed the co-circulation of three distinct Alpha-CoVs within a Miniopterus schreibersii (M. schreibersii) fecal sample and the presence of a SARS-CoV-related strain in a Rhinolophus hipposideros (R. hipposideros) fecal sample. Both the Alpha-CoVs and the Beta-CoVs were most closely related to European strains. These findings confirm the widespread circulation of diverse CoVs in Italian bats and underscore the need for continued molecular epidemiological surveillance to unravel the full extent of CoV heterogeneity in European wildlife.","PeriodicalId":7955,"journal":{"name":"Animals","volume":"16 20","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13700689/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149260846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Estimation of Greenhouse Gas Emission Factor During Hanwoo Manure Composting. 韩雨粪肥堆肥过程中温室气体排放因子的估算

IF 3.2 2区农林科学

Animals Pub Date : 2026-10-21 DOI: 10.3390/ani16203322

Woo Je Lee, Geun Woo Park, Sung Chul Seo, Ki-Youn Kim, Jung Kon Kim

{"title":"Estimation of Greenhouse Gas Emission Factor During Hanwoo Manure Composting.","authors":"Woo Je Lee, Geun Woo Park, Sung Chul Seo, Ki-Youn Kim, Jung Kon Kim","doi":"10.3390/ani16203322","DOIUrl":"10.3390/ani16203322","url":null,"abstract":"Accurate estimation of climate-altering gases, such as methane and nitrous oxide, from livestock waste treatment is critical for establishing national inventories. This study aims to monitor and analyze the emission characteristics of these gases during the treatment process at an operational Hanwoo (Korean cattle for beef) farm in Republic of Korea to develop country-specific metrics. A full-scale compost shed was modified into a 2400 m3 flux chamber equipped with fans to control airflow at 183 m3·min-1. Real-time gas concentrations were measured using enhanced-performance laser-based analyzers across summer, autumn, and winter. The results indicated that mean emissions for both methane and nitrous oxide were highest in summer, followed by winter, and lowest in autumn. Daily patterns revealed higher hourly mean emissions during the morning and afternoon. The final estimated emission factors were 1.28 kgCH4·head-1·yr-1 and 0.039 kgN2O· head-1·yr-1. These values are relatively lower than those reported in previous studies. These values were lower than those reported in several previous studies. Differences in facility scale and operating conditions may partly explain this result; however, potential underestimation due to non-uniform airflow should also be considered.","PeriodicalId":7955,"journal":{"name":"Animals","volume":"16 20","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13700611/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149260780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Free-Ranging Cat Predation on Wildlife in East Asia: A Scoping Review of Ecological Evidence and Knowledge Gaps. 东亚野生动物的自由放养猫捕食：生态学证据和知识空白的范围审查。

IF 3.2 2区农林科学

Animals Pub Date : 2026-10-21 DOI: 10.3390/ani16203321

Michelle Zhibing Zhou, Christian S Wright

{"title":"Free-Ranging Cat Predation on Wildlife in East Asia: A Scoping Review of Ecological Evidence and Knowledge Gaps.","authors":"Michelle Zhibing Zhou, Christian S Wright","doi":"10.3390/ani16203321","DOIUrl":"10.3390/ani16203321","url":null,"abstract":"Free-ranging domestic cats (Felis catus) interact with wildlife through predation and other ecological pathways, yet evidence of their associated effects has not been comprehensively synthesized in East Asia. This scoping review mapped empirical evidence on free-ranging cat predation and associated ecological effects in East Asia, characterized its geographic distribution and inferential strength, and identified knowledge gaps. Five databases, including region-specific databases, were searched through June 2026, and reference lists of included full-text sources were hand-searched. Of 9326 records identified, 31 sources met the inclusion criteria: 28 from the pre-specified East Asian region and 3 global or multinational sources with independently extractable East Asia-specific data. Diet- and carcass-based studies documented consumption of diverse wildlife taxa, whereas fewer evaluated prey abundance or other population-level outcomes. Reported predation estimates varied substantially among studies, reflecting differences in cat category, ecological setting, and methodology. Evidence was concentrated in Japan, with the clearest conservation concerns reported from Japanese island ecosystems involving endemic or IUCN-listed threatened species. Human-mediated factors and management practices were recurrent contextual themes; however, direct evidence linking these factors to wildlife outcomes was limited. Overall, the evidence base was geographically and methodologically uneven, underscoring the need for standardization, broader regional coverage, and improved documentation.","PeriodicalId":7955,"journal":{"name":"Animals","volume":"16 20","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13700886/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149260916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IF 3.2 2区农林科学

Animals Pub Date : 2026-10-21 DOI: 10.3390/ani16203317

Réjane Kerbrat-Copy, Océane Thérier, Marion Fusellier

{"title":"Age-Related Decrease in Interthalamic Adhesion Thickness in Cats Without Apparent Cognitive Dysfunction.","authors":"Réjane Kerbrat-Copy, Océane Thérier, Marion Fusellier","doi":"10.3390/ani16203317","DOIUrl":"10.3390/ani16203317","url":null,"abstract":"Feline cognitive dysfunction syndrome (FCDS) is an age-associated disorder for which objective imaging markers remain limited. This study evaluated the association between age and interthalamic adhesion thickness (IAT) in cats without apparent cognitive dysfunction and described IAT across feline life stages. MRI examinations from 112 client-owned cats aged 4 months to 18 years were retrospectively reviewed. Cats were classified as kittens (<1 year), young (1 to < 7 years), mature (7 to <11 years) and senior (≥11 years). Cats with intracranial lesions or reported behavioral changes suggestive of cognitive dysfunction were excluded. Two blinded observers measured IAT on transverse T1W and T2W images. Analyses included linear regression adjusted for brain height, brain width, sex/neuter status, breed group, and scanner; exploratory quadratic age modeling; and comparisons among life stages. Interobserver agreement was high. Age was negatively associated with IAT on T1W (B = -0.071 mm/year) and T2W images (B = -0.067 mm/year; both p < 0.001). This association persisted after multivariable adjustment (T1W: B = -0.074 mm/year; T2W: B = -0.067 mm/year; both p < 0.001) and after excluding kittens or brachycephalic cats. Senior cats had lower IAT than all other life stages on both sequences. A quadratic term suggested a steeper decline at older ages on T1W images only; this finding remains exploratory. These findings support a group-level association between age and IAT, rather than individual-level diagnostic use. Its potential diagnostic value for FCDS requires prospective evaluation with standardized behavioral assessment and age-matched controls.","PeriodicalId":7955,"journal":{"name":"Animals","volume":"16 20","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13700864/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149260939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immune Response of the Pacific Oyster Magallana gigas Following Shell Damage. 太平洋牡蛎壳损伤后的免疫反应。

IF 3.2 2区农林科学

Animals Pub Date : 2026-10-21 DOI: 10.3390/ani16203310

Daria S Lavrichenko, Elizaveta A Bogacheva, Viktoria A Uppe, Ekaterina A Vodiasova, Elina S Chelebieva, Ekaterina S Kladchenko

{"title":"Immune Response of the Pacific Oyster Magallana gigas Following Shell Damage.","authors":"Daria S Lavrichenko, Elizaveta A Bogacheva, Viktoria A Uppe, Ekaterina A Vodiasova, Elina S Chelebieva, Ekaterina S Kladchenko","doi":"10.3390/ani16203310","DOIUrl":"10.3390/ani16203310","url":null,"abstract":"Shell integrity is a key determinant of health in cultured bivalves; however, its damage may affect physiological and immune processes associated with repair. This study evaluated the effects of a single non-penetrating shell damage on cellular immune responses and immune-related gene expression in the mantle of the Pacific oyster Magallana gigas. Over 14 days post-damage, reactive oxygen species (ROS) and reactive nitrogen species (RNS) production, phagocytic activity, adhesion, and aggregation of hemocytes were assessed. Relative expression of tumor necrosis factor (TNF-1, TNF-2), interleukin-17 (IL-17-3, IL-17-6), and Toll receptor (Toll-1) genes was also determined in mantle tissue. The damage induced predominantly temporal alterations in hemocyte functional activity. During the first two days, ROS production decreased, while RNS production remained unchanged; phagocytic activity increased on day 1 and decreased on day 2. More prolonged changes were observed in hemocyte adhesion and aggregation. Of the genes examined, only TNF-2 showed a significant change, with decreased expression on day 7. These results show that a single non-penetrating shell damage primarily induces a broad stress-associated physiological response, which transiently modulates cellular immune parameters and local regulation mechanisms. By the end of the experiment, most parameters did not differ from the control, although certain changes persisted during shell repair.","PeriodicalId":7955,"journal":{"name":"Animals","volume":"16 20","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13700679/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149260581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanoscale Characterization of Microscopically Layered Bone in the Russian Sturgeon Pectoral Fin Spine: A 3D FIB-SEM Study. 俄罗斯鲟鱼胸鳍骨微观层状骨的纳米级表征：3D FIB-SEM研究。

IF 3.2 2区农林科学

Animals Pub Date : 2026-10-21 DOI: 10.3390/ani16203318

Esteban Marroquín-Arroyave, Joshua Milgram

{"title":"Nanoscale Characterization of Microscopically Layered Bone in the Russian Sturgeon Pectoral Fin Spine: A 3D FIB-SEM Study.","authors":"Esteban Marroquín-Arroyave, Joshua Milgram","doi":"10.3390/ani16203318","DOIUrl":"10.3390/ani16203318","url":null,"abstract":"Dermal bone, the earliest known form of bone, develops without a cartilage precursor and persists in extant fish species. The pectoral fin spine (PFS) is a dermal bone located at the leading edge of the pectoral fin of sturgeons, where it is exposed to loading associated with locomotion. The peripheral region and dermal osteons of the PFS both appear layered at lower magnification; however, collagen organization in load-bearing fish skeletal elements remains incompletely characterized at the nanoscale. The aim of this study is to characterize the 3D nanostructure of the layered peripheral and osteonal bone forming the PFS in Russian sturgeons using focused ion beam-scanning electron microscopy (FIB-SEM). In the dermal osteons, bone forms concentric layers around a central canal at lower magnification, and at the nanoscale, the calcified collagen fibrils within these layers have preferential orientations that change in a clockwise-anticlockwise fanning pattern, consistent with lamellar bone. In contrast, the examined peripheral bone, which also appears layered at low magnification, is composed mainly of unidirectional calcified collagen fibrils aligned with the long axis of the PFS. Within the examined peripheral volumes, no comparable nanoscale lamellar pattern was detected, and the predominant fibril organization was consistent with parallel-fibered bone. Groups of voids associated with unidentified fibrillar material were also observed in the examined peripheral volumes; the voids were oriented approximately normal to the long axis of the PFS. We conclude that regions appearing layered at lower magnification can differ markedly at the nanoscale, and that the lamellar organization seen in dermal osteons raises questions about its evolutionary origin.","PeriodicalId":7955,"journal":{"name":"Animals","volume":"16 20","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13700704/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149260928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0