Animals最新文献 - Book学术

Functional Requirement of Niacinamide for Blood Profiles, Antioxidant Status, and Intestinal Health in Finishing Pigs Fed a Low-Protein Diet. 低蛋白饲粮对育肥猪血液、抗氧化状态和肠道健康的烟酰胺功能需求

IF 2.7 2区农林科学

Animals Pub Date : 2025-06-19 DOI: 10.3390/ani15121813

Yan Zhao, Fangli Tang, Yunlong Shi, Qinyu Tan, Qingxin Ju, Ziyi Yang, Guanqing Yang, Pengfei Gao, Sung Woo Kim, Lin Xi, Guoqing Cao, Bugao Li

{"title":"Functional Requirement of Niacinamide for Blood Profiles, Antioxidant Status, and Intestinal Health in Finishing Pigs Fed a Low-Protein Diet.","authors":"Yan Zhao, Fangli Tang, Yunlong Shi, Qinyu Tan, Qingxin Ju, Ziyi Yang, Guanqing Yang, Pengfei Gao, Sung Woo Kim, Lin Xi, Guoqing Cao, Bugao Li","doi":"10.3390/ani15121813","DOIUrl":"10.3390/ani15121813","url":null,"abstract":"This study investigated the effects of dietary niacinamide supplementation on blood parameters, antioxidant status, and intestinal health in finishing pigs fed low-protein diets. Sixty-four pigs (Duroc × Landrace × Yorkshire; 80.4 ± 0.1 kg) were randomly allocated to four dietary treatments supplemented with 30 (NAM30), 130 (NAM130), 230 (NAM230), and 330 (NAM330) mg/kg niacinamide for 30 days. Each treatment had four replicate pens and four pigs per pen. Growth performance was not significantly affected. However, the NAM130 group showed higher (p < 0.05) hemoglobin levels, reduced (p < 0.05) serum malondialdehyde and interleukin-1β (IL-1β) concentrations, and altered intestinal microbiota composition, including lower Streptococcus abundance (p < 0.05). Serum alanine aminotransferase levels increased quadratically (p < 0.05) with niacinamide supplementation, with a breakpoint at approximately 221 mg/kg. These results suggest that dietary supplementation with 130 mg/kg niacinamide improves antioxidant status, modulates inflammation, and supports intestinal microbial balance, with a safety threshold to avoid hepatic stress.","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 12","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12189497/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144493485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preliminary Study of the Antimicrobial Capacity of the Cutaneous Mucus and Smear Cytology of the Epidermis in a Population of European eels (Anguilla anguilla, Linnaeus 1758). 欧洲鳗鲡皮肤黏液抗菌能力及表皮涂片细胞学的初步研究（Anguilla Anguilla, Linnaeus 1758）。

IF 2.7 2区农林科学

Animals Pub Date : 2025-06-19 DOI: 10.3390/ani15121810

Enrico Volpe, Sara Ciulli, Maria Morini, Laura Gentile, Antonio Casalini, Chiara Gentilezza, Luciana Mandrioli

{"title":"Preliminary Study of the Antimicrobial Capacity of the Cutaneous Mucus and Smear Cytology of the Epidermis in a Population of European eels (Anguilla anguilla, Linnaeus 1758).","authors":"Enrico Volpe, Sara Ciulli, Maria Morini, Laura Gentile, Antonio Casalini, Chiara Gentilezza, Luciana Mandrioli","doi":"10.3390/ani15121810","DOIUrl":"10.3390/ani15121810","url":null,"abstract":"The skin and its products, such as the mucus, represent an important defense mechanism against infection by pathogens. Various environmental conditions can alter mucus composition and/or cells embedded in this matrix. The analysis of skin mucus is considered a useful method to evaluate the biological response of fish to stimuli. The mucus and cells can be considered suitable non-invasive biomarkers. With this preliminary study, an antibacterial capacity method was applied to cutaneous mucus samples of a European eel population. This standardized method highlighted the ability of the mucus to significantly counteract the proliferation of two bacteria, the opportunistic pathogen Aeromonas hydrophila and the primary pathogen Aeromonas salmonicida. The cytological method using skin scraping has proven to be a useful non-invasive tool, having allowed the highlighting of the two most represented cellular populations of the cutaneous covering, the epidermal cells and the goblet cells, in combination with mucous strands. Cytological findings did not disclose pathologic elements in the mucus. A broader use of these two non-invasive microbiological and cytological methods can provide useful information on fish health, indirectly contributing to the conservation of the species.","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 12","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12189166/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144493582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preclinical Evaluation of Fenbendazole for Controlling Gyrodactylus kobayashii (Monogenea, Gyrodactylidae) in Goldfish: Dose Optimization and Safety Assessment. 芬苯达唑防治金鱼小林回肠虫的临床前评价：剂量优化及安全性评价。

IF 2.7 2区农林科学

Animals Pub Date : 2025-06-19 DOI: 10.3390/ani15121811

Jing Dong, Jiangtao Li, Yongtao Liu, Qiuhong Yang, Ning Xu, Xiaohui Ai, Shun Zhou

{"title":"Preclinical Evaluation of Fenbendazole for Controlling Gyrodactylus kobayashii (Monogenea, Gyrodactylidae) in Goldfish: Dose Optimization and Safety Assessment.","authors":"Jing Dong, Jiangtao Li, Yongtao Liu, Qiuhong Yang, Ning Xu, Xiaohui Ai, Shun Zhou","doi":"10.3390/ani15121811","DOIUrl":"10.3390/ani15121811","url":null,"abstract":"This preclinical study investigated the efficacy and safety of fenbendazole, a broad-spectrum benzimidazole anthelmintic, for the treatment of Gyrodactylus kobayashii in goldfish (Carassius auratus). In vivo bath treatments demonstrated potent, dose-dependent anthelmintic efficacy, achieving 98.58% efficacy at a concentration of 0.02 mg/L and a 48 h EC50 of 0.006 mg/L. A short-duration (6 h) bath at 0.06 mg/L, followed by an 18 h recovery period in dechlorinated water, resulted in complete parasite elimination. However, acute toxicity assay indicated a relatively narrow safety margin for prolonged bath treatments, with a 96 h LC50 of 0.039 mg/L, highlighting the need for caution when employing extended bath treatments. Oral administration of fenbendazole at 20 mg/kg body weight for three consecutive days resulted in an efficacy of 83.35%, which increased to 96.28% by seven days post-treatment. Safety evaluations revealed this regimen induced transient oxidative stress and mild, reversible histopathological alterations in the liver and gills. Biochemical and histological markers indicated a recovery trend, approaching baseline levels by 15 days post-treatment. These findings suggested that oral fenbendazole is an effective and relatively safe anthelmintic treatment against G. kobayashii in goldfish. This study underscores the potential of drug repurposing as an effective strategy for developing novel anthelmintic agents in aquaculture.","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 12","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12189257/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144493601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Mertz et al. Interdisciplinary Animal Research Ethics-Challenges, Opportunities, and Perspectives. Animals 2024, 14, 2896. 更正：Mertz等人。跨学科动物研究伦理——挑战、机遇和观点。动物学报，2024,14,2896。

IF 2.7 2区农林科学

Animals Pub Date : 2025-06-19 DOI: 10.3390/ani15121803

Marcel Mertz, Tatiana Hetzel, Karla Alex, Katharina Braun, Samuel Camenzind, Rita Dodaro, Svea Jörgensen, Erich Linder, Sara Capas-Peneda, Eva Ingeborg Reihs, Vini Tiwari, Zorana Todorović, Hannes Kahrass, Felicitas Selter

引用次数: 0

Sperm Membrane: Molecular Implications and Strategies for Cryopreservation in Productive Species. 精子膜：生殖物种低温保存的分子意义和策略。

IF 2.7 2区农林科学

Animals Pub Date : 2025-06-19 DOI: 10.3390/ani15121808

Macarena Castro, Karla Leal, Felipe Pezo, María José Contreras

{"title":"Sperm Membrane: Molecular Implications and Strategies for Cryopreservation in Productive Species.","authors":"Macarena Castro, Karla Leal, Felipe Pezo, María José Contreras","doi":"10.3390/ani15121808","DOIUrl":"10.3390/ani15121808","url":null,"abstract":"Sperm cryopreservation is a fundamental reproductive biotechnology, enabling the long-term storage of genetic material and supporting assisted reproduction programs. Despite its widespread application, the process is associated with significant limitations due to the cryo-induced cellular damage that occurs during freezing and thawing. These injuries primarily affect the plasma membrane, nuclear DNA, and motility, thereby compromising the fertilizing potential of spermatozoa. Furthermore, interspecies variability in terms of cryo-sensitivity poses a major challenge to the development of standardized cryopreservation protocols. Recent advances have focused on mitigating cryodamage through the use of various strategies. The inclusion of antioxidants in cryopreservation media has proven effective in reducing oxidative stress, thereby enhancing cellular protection. Similarly, the addition of lipid-based supplements contributes to membrane stabilization, improving post-thaw sperm viability and functionality. Moreover, the application of omics technologies, such as transcriptomics and proteomics, has facilitated a deeper understanding of molecular damage and protective responses, paving the way for the development of tailored, species-specific protocols. These integrated approaches optimize cryopreservation conditions, maximizing post-thaw survival and the fertilizing capacity of sperm. Enhancing cryopreservation techniques not only improves the outcomes of assisted reproductive technologies, but also plays a crucial role in the conservation of genetically valuable livestock species. In conclusion, the integration of biotechnological and molecular tools holds significant promise for overcoming the current limitations and advancing the efficacy of sperm cryopreservation.","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 12","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12189332/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144493590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of Stress in Dogs Under Cancer Therapy via Faecal Cortisol Metabolite Analysis: A Pilot Study. 通过粪便皮质醇代谢物分析评估癌症治疗犬的压力：一项初步研究。

IF 2.7 2区农林科学

Animals Pub Date : 2025-06-19 DOI: 10.3390/ani15121809

Christina Ziegerhofer, Alexander Tichy, Miriam Kleiter, Birgitt Wolfesberger, Rupert Palme

{"title":"Assessment of Stress in Dogs Under Cancer Therapy via Faecal Cortisol Metabolite Analysis: A Pilot Study.","authors":"Christina Ziegerhofer, Alexander Tichy, Miriam Kleiter, Birgitt Wolfesberger, Rupert Palme","doi":"10.3390/ani15121809","DOIUrl":"10.3390/ani15121809","url":null,"abstract":"Stress reactions play an important role in animals' ability to cope with various situations. Glucocorticoids are measured as a stress parameter, and analysis of their faecal metabolites has proven to be a good method for evaluating long term stress. We hypothesised that dogs suffering from cancer would have a higher stress level during cancer therapy, which would be reflected in higher levels of faecal cortisol metabolites (FCMs). Faeces were collected from 40 dogs receiving radiation or chemotherapy before and during the treatment, and from 53 healthy dogs, who served as a control group. FCMs were extracted and quantified by a cortisol enzyme immunoassay. The results showed that the stress levels were not significantly higher in the cancer patients before therapy compared to those in the control group. Additionally, there were no significant differences in the FCM concentrations of dogs at specific time points during chemotherapy or radiotherapy. Therefore, suspected stress should not be a criterion to exclude animals from cancer treatment. Such a treatment seems ethically justifiable if it is expected to provide benefits and improvement in the quality of life for patients.","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 12","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12189573/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144493502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The In Vitro Effects of Choline on Non-Esterified Fatty Acid-Treated Bovine Peripheral Blood Leukocytes. 胆碱对非酯化脂肪酸处理牛外周血白细胞的体外影响。

IF 2.7 2区农林科学

Animals Pub Date : 2025-06-19 DOI: 10.3390/ani15121814

Cheng-Yan Li, Yueh-Tung Chen, Tossapol Moonmanee, Jacky Peng-Wen Chan, Chien-Kai Wang

{"title":"The In Vitro Effects of Choline on Non-Esterified Fatty Acid-Treated Bovine Peripheral Blood Leukocytes.","authors":"Cheng-Yan Li, Yueh-Tung Chen, Tossapol Moonmanee, Jacky Peng-Wen Chan, Chien-Kai Wang","doi":"10.3390/ani15121814","DOIUrl":"10.3390/ani15121814","url":null,"abstract":"The transition period is defined as 3 weeks around parturition, involving the rapid increase in the energy demand, promoting adipose mobilization and non-esterified fatty acid (NEFA) release. High NEFA levels might cause oxidative stress and associated health risks, including the disruption of the immune capability of peripheral leukocytes. Nutrient supplementation of choline, which improves cellular lipid metabolism and controls lipid oxidation, potentially maintains the integrity of peripheral leukocytes and alleviates the impacts of increased NEFAs. This study investigated the effects of choline on bovine peripheral blood leukocytes (PBLs) treated with high levels of NEFAs. Peripheral blood mononuclear cells (PBMCs) and polymorphonuclear leukocytes (PMNs) were isolated from dry cows, and treated with 1 mM NEFA in combination with 0, 4, or 12 μM choline. The expression of pro-inflammatory cytokines and oxidative stress indicators was determined. This study demonstrates that 1 mM NEFA induces lipid oxidation and pro-inflammatory cytokine expression in PBLs. Supplementation with 4 μM and 12 μM choline significantly reduced NEFA-induced lipid oxidation; however, it did not affect pro-inflammatory cytokine mRNA expression. In conclusion, choline supplementation may help alleviate NEFA-induced oxidative stress in bovine peripheral blood leukocytes, highlighting its potential as a functional supplement during the transition period. However, it had no observable effect on mitigating pro-inflammatory responses, indicating that additional strategies may be needed to address NEFA-induced immune activation.","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 12","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12189240/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144493625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of Solid Lipid Microcapsule Matrix for Enhanced Release and Bioavailability of L-Lysine in Swine. 提高l -赖氨酸在猪体内释放和生物利用度的固体脂质微胶囊基质优化。

IF 2.7 2区农林科学

Animals Pub Date : 2025-06-19 DOI: 10.3390/ani15121806

Costanza Bonnici, Maria Federica Marchesi, Martina Felici, Federico Ghiselli, Roberta Majer, Benedetta Tugnoli, Guglielmo Gallina, Andrea Piva, Ester Grilli

{"title":"Optimization of Solid Lipid Microcapsule Matrix for Enhanced Release and Bioavailability of L-Lysine in Swine.","authors":"Costanza Bonnici, Maria Federica Marchesi, Martina Felici, Federico Ghiselli, Roberta Majer, Benedetta Tugnoli, Guglielmo Gallina, Andrea Piva, Ester Grilli","doi":"10.3390/ani15121806","DOIUrl":"10.3390/ani15121806","url":null,"abstract":"L-lysine (L-Lys) is the first-limiting amino acid in swine nutrition, but free-form supplements exhibit poor intestinal absorption, reducing their bioavailability. This study aimed to enhance the gastric retention, controlled intestinal release, and systemic availability of L-Lys by optimizing solid lipid microcapsules (SLMs). SLMs were formulated using hydrogenated triglycerides (C16:0 or C18:1), free fatty acids, and varying emulsifier concentrations. Gastric retention and intestinal release were evaluated in vitro under simulated gastrointestinal conditions (a pepsin buffer at pH 5.0 for 2 h, followed by a pancreatin buffer at pH 6.5 for up to 8 h at 39 °C). SLMs with hydrogenated triglycerides showed significantly higher gastric retention (94-95%) than those with free fatty acids (48%). Specifically, C16:0 triglyceride-based SLMs achieved 74% intestinal release, which was enhanced to 90% with 1% emulsifier. This refined formulation was subsequently evaluated in vivo using weaned pigs (three groups; n = 4) fed a basal cornmeal diet. The treatments included a single oral administration of saline solution (placebo), free L-Lys (0.17 g/kg BW), or L-Lys SLMs (0.38 g/kg BW, equally providing L-Lys at 0.17 g/kg BW). The SLMs delayed the L-Lys plasma peak (T. max. 3-4 h vs. 1 h) and significantly increased the total L-Lys amount in the plasma over 24 h, demonstrating the enhanced relative bioavailability of encapsulated L-Lys.","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 12","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12189550/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144493597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effects of the Lactation Period, Mare Age, and Foaling on the Chemical and Physical Composition of Milk from Kazakh Mares Kept Under Natural Pasture Conditions. 哺乳期、马龄和产驹对自然放牧条件下哈萨克母马奶理化成分的影响

IF 2.7 2区农林科学

Animals Pub Date : 2025-06-19 DOI: 10.3390/ani15121817

Maxat Toishimanov, Olzhas Zhanten, Rakhim Kanat, Indira Beishova, Vadim Ulyanov, Tolegen Assanbayev, Tlekbol Sharapatov, Dias Daurov, Ainash Daurova, Zagipa Sapakhova, Askar Nametov, Malika Shamekova

{"title":"The Effects of the Lactation Period, Mare Age, and Foaling on the Chemical and Physical Composition of Milk from Kazakh Mares Kept Under Natural Pasture Conditions.","authors":"Maxat Toishimanov, Olzhas Zhanten, Rakhim Kanat, Indira Beishova, Vadim Ulyanov, Tolegen Assanbayev, Tlekbol Sharapatov, Dias Daurov, Ainash Daurova, Zagipa Sapakhova, Askar Nametov, Malika Shamekova","doi":"10.3390/ani15121817","DOIUrl":"10.3390/ani15121817","url":null,"abstract":"This study aimed to evaluate the chemical composition of milk from Kazakh mares kept under free-grazing conditions on natural pasture without supplementary feeding. Fifty Kazakh mares were aged 3 to 13 years, kept in herds, and categorized by age, number of foalings, and lactation stage for statistical analysis. Milk samples were collected, chilled, and analyzed on the same day using a Milkoscan FT2. The composition of fat, protein, lactose, total solids, casein, and other components was examined. Statistical analyses, including ANOVA, PCA, CDA, and Pearson correlation analysis, were performed using JMP 17 Pro to assess the effects of lactation stage, mare age, and foaling number and to identify key relationships. The results showed that milk composition was significantly influenced by these factors. Milk from the late-lactation stage contained higher protein, lactose, and total nutrients, while milk from the early-lactation stage had a higher sugar content and acidity. Older mares and those with higher foalings produced milk with higher concentrations of glucose and galactose, while younger mares and those with fewer foalings produced milk with higher fat. A key aspect of this experiment was that all mares grazed exclusively on natural pastures without supplementation, allowing for an objective assessment of their inherent productive potential. These findings deepen the understanding of mare milk composition dynamics in Kazakh breed horses under extensive management, supporting its potential for organic dairy production.","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 12","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12189648/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144493622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diagnostic Approach and Pathological Characterization of Metastatic Intrahepatic Cholangiocarcinoma in a Captive Puma (Puma concolor). 圈养美洲狮（美洲狮）转移性肝内胆管癌的诊断方法和病理特征。

IF 2.7 2区农林科学

Animals Pub Date : 2025-06-19 DOI: 10.3390/ani15121821

Elisa Mazzotta, Claudia Zanardello, Giovanni De Zottis, Antonio Barberio, Mery Campalto, Federico Martignago, Giulia Maria De Benedictis, Carlo Guglielmini, Francesca Zanusso, Greta Foiani

{"title":"Diagnostic Approach and Pathological Characterization of Metastatic Intrahepatic Cholangiocarcinoma in a Captive Puma (Puma concolor).","authors":"Elisa Mazzotta, Claudia Zanardello, Giovanni De Zottis, Antonio Barberio, Mery Campalto, Federico Martignago, Giulia Maria De Benedictis, Carlo Guglielmini, Francesca Zanusso, Greta Foiani","doi":"10.3390/ani15121821","DOIUrl":"10.3390/ani15121821","url":null,"abstract":"A 16-year-old captive male puma (Puma concolor) presented with progressive weight loss and acute mild gastrointestinal symptoms. Clinical examination, and diagnostic imaging revealed abdominal, pleural, and pericardial effusion and nodular masses in multiple organs. Due to the clinical condition, multiple organ involvement, and a poor prognosis, compassionate euthanasia was chosen. Necropsy revealed abundant cavitary effusions and multiple yellow-white neoplastic masses involving the left hepatic lobes. Metastatic nodules were observed throughout the peritoneum, pericardium, kidney, spleen, and spermatic cord. Histologically, the tumor was characterized by tubular and acinar structures embedded in a desmoplastic stroma. Neoplastic cells were positive for pan-cytokeratins (CKs), CK7, and, to a lesser extent, CK20. The gross, histologic, and immunohistochemical findings were consistent with intrahepatic metastatic cholangiocarcinoma.","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 12","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12189588/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144493535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0