Animals最新文献

{"title":"Environmental DNA and Hydroacoustic Surveys for Monitoring the Spread of the Invasive European Catfish (<i>Silurus glanis</i> Linnaeus, 1758) in the Guadalquivir River Basin, Spain.","authors":"Ruth Coya, Amadora Rodríguez-Ruiz, Álvaro Fueyo, Carlos Orduna, Laura Miralles, Ilaria de Meo, Trinidad Pérez, Juan Ramón Cid, Carlos Fernández-Delgado, Lourdes Encina, Yaisel J Borrell, Carlos Granado-Lorencio","doi":"10.3390/ani15020285","DOIUrl":"10.3390/ani15020285","url":null,"abstract":"<p><p>The European catfish (<i>Silurus glanis</i> Linnaeus, 1758) was introduced into the Ebro Basin in Spain in 1974 for recreational fishing. Since then, the species has spread throughout the country's river basins, reaching the Iznájar Reservoir (Guadalquivir River Basin) in 2011. This area is of great ecological and economic relevance, especially as it includes the Doñana National Park, one of the most important nature reserves in Europe. Recently, the presence of catfish has been reported in the lower reaches of the river. In this work, we used non-invasive vertical and horizontal hydroacoustic surveys and environmental DNA river sampling to unravel the actual distribution and dispersal pattern of the species in the lower Guadalquivir River. The hydroacoustic profiles and the species-specific detections by real-time PCR (qPCR) and droplet digital PCR (ddPCR) showed that these non-invasive methods allow the detection and quantification of catfish and provide valuable information on the species' presence. We have confirmed the presence of catfish in most of the study area, including downstream areas of the Guadalquivir Basin. The results suggest the possibility of other isolated introductions and/or human-mediated movements of specimens, and imply that a coordinated catfish prevention and mitigation strategy is therefore urgently needed.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11761632/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome Analysis Reveals the Molecular Mechanism of PLIN1 in Goose Hierarchical and Pre-Hierarchical Follicle Granulosa Cells.","authors":"Hua He, Yueyue Lin, Xi Zhang, Hengli Xie, Zhujun Wang, Shenqiang Hu, Liang Li, Hehe Liu, Chunchun Han, Lu Xia, Jiwei Hu, Jiwen Wang, Lin Liao, Xin Yuan","doi":"10.3390/ani15020284","DOIUrl":"10.3390/ani15020284","url":null,"abstract":"<p><p><i>PLIN1</i>, a member of the PAT family, is expressed in both adipocytes and steroidogenic cells. In this study, we used cell transfection technology combined with transcriptome sequencing to investigate the regulatory mechanism of <i>PLIN1</i> in goose follicular GCs. Gene Ontology (GO) analysis revealed that in the four groups (phGC: over_vs_over-NC; hGC: over_vs_over-NC; phGC: si_vs_si-NC; hGC: si_vs_si-NC), most differentially expressed genes (DEGs) were significantly enriched (<i>p</i> < 0.05) in pathways related to biological processes (BPs), particularly those associated with the regulation of cellular lipid metabolism and oxidative stress. KEGG analysis further identified significant enrichment (<i>p</i> < 0.05) in pathways related to cell apoptosis and the cell cycle. A joint analysis of KEGG and PPI on the upregulated and downregulated DEGs revealed that the TGF-β signaling pathway was the only pathway significantly enriched among both upregulated and downregulated DEGs after <i>PLIN1</i> overexpression in hGCs and phGCs. Based on these findings, we hypothesize that <i>PLIN1</i> overexpression may promote granulosa cell proliferation and apoptosis by activating the TGF-β signaling pathway in goose follicular GCs. Additionally, nine potential candidate genes were identified: <i>PPARγ</i>, <i>MGLL</i>, <i>PTEN</i>, <i>BAMBI</i>, <i>BMPR2</i>, <i>JUN</i>, <i>FST</i>, <i>ACSF3</i>, and <i>ACSL4</i>. These results address a significant research gap concerning the role of this gene in granulosa cells and contribute to the understanding of its molecular regulatory mechanisms.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11761271/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbiological Analysis Conducted on Raw Milk Collected During Official Sampling in Liguria (North-West Italy) over a Ten-Year Period (2014-2023).","authors":"Sara Antonia Chiarlone, Andrea Gori, Serena Ravetta, Andrea Armani, Lisa Guardone, Francesca Pedonese, Salvatore Bavetta, Caterina Fiannacca, Nicola Pussini, Cristiana Maurella, Elisabetta Razzuoli","doi":"10.3390/ani15020286","DOIUrl":"10.3390/ani15020286","url":null,"abstract":"<p><p>Milk has been consumed by humans for thousands of years for its nutritional properties. In recent years, raw milk demand has increased, valued for its authenticity and connection to local traditions. In Italy, the sale of raw milk is allowed exclusively through direct sale from the producing farm to the final consumer, either at the producing farm itself or through vending machines. However, the consumption of raw milk is not without risks. Among these, microbiological ones are relevant. These can lead to severe symptoms, particularly in vulnerable populations. For this reason, although consumers are advised to boil raw milk before consumption, producing farms in Italy are required to meet the microbiological criteria outlined in the Provision of 25 January 2007. In this retrospective study, the results of the analyses performed on 355 raw milk samples collected in Liguria between 2014 and 2023 for the detection of <i>Campylobacter</i> spp., <i>Salmonella</i> spp., <i>Listeria monocytogenes</i>, <i>Staphylococcus aureus</i>, and <i>Escherichia coli</i> O157 were analysed to better characterise the associated risk for consumers. The samples were collected during official controls by the local veterinary health services at vending machines of seven producing farms. Overall, six samples tested positive for <i>C. jejuni</i>, while only one sample tested positive for <i>Salmonella enterica</i> subsp. <i>enterica</i>, Serovar Veneziana. <i>Listeria monocytogenes</i>, <i>S. aureus</i>, and <i>E. coli</i> O157 were never responsible for non-compliances. Interestingly, three of the six samples positive for <i>C. jejuni</i> derived from the same producer. In farms where positive samples were detected, certain structural and/or operational non-compliances were identified. It can be concluded that, although the scenario in question does not present any cause for concern, it is nevertheless essential to implement a series of preventive measures in order to guarantee the safety of raw milk. These measures include the implementation of biosecurity practices, the maintenance of strict hygiene protocols during milking, and the adherence to the cold chain distribution protocol until the final stage of distribution.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11763045/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of Vaccination Regimens on Immune Responses Using PED Replicon Vaccine: A Field Trial in PED-Negative and PED-Positive Thai Swine Farms.","authors":"Chaitawat Sirisereewan, Thanh Che Nguyen, Nanthiya Iampraphat, Hongyao Lin, Leonardo Ellerma, Pisit Sirithanyakul, Roongtham Kedkovid, Roongroje Thanawongnuwech","doi":"10.3390/ani15020273","DOIUrl":"10.3390/ani15020273","url":null,"abstract":"<p><p>Porcine epidemic diarrhea virus (PEDV) is an economically important pathogen of swine, causing severe diarrhea in neonates with high morbidity and mortality. Vaccination is a key strategy for PEDV control, but optimizing regimens based on herd status is essential for improving immunity and protection. This study evaluated immune responses to different vaccination protocols using a PED replicon vaccine (PED-RP) in Thai swine farms with varying PED statuses. Two field trials were conducted: Experiment 1 (<i>n</i> = 120) on a PED-negative farm used a killed vaccine followed by PED-RP, while Experiment 2 (<i>n</i> = 42) on a PED-positive farm used a modified-live vaccine (MLV) followed by PED-RP. In Experiment 1, gilts boosted with PED-RP during gestation showed significantly higher colostrum IgA levels compared to those receiving two doses of the killed vaccine. In Experiment 2, priming with MLV followed by PED-RP resulted in increased neutralizing antibody titers and seropositivity, outperforming the MLV-only regimen after acclimatization. These findings suggest that using PED-RP as a booster can enhance serum and lactogenic immunity, providing a promising strategy for improving immune responses in both PED-negative and PED-positive herds. The study highlights the importance of tailored vaccination strategies to optimize herd immunity and protect piglets from PEDV.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11759142/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143036000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Complex Probiotics Suppress Inflammation by Regulating Intestinal Metabolites in Kittens.","authors":"Shimin Zhu, Musu Zha, Yanan Xia","doi":"10.3390/ani15020272","DOIUrl":"10.3390/ani15020272","url":null,"abstract":"<p><p>Cats are popular companions for humans, and their health is of importance to a growing number of pet owners. The juvenile period is a critical stage of cat growth; in particular, the metabolic activity of the intestinal microbiome of kittens is critical for processing nutrients and supporting overall health. In this study, the effect of complex probiotics on the intestinal health of kittens was explored through a metabolomics analysis. Twenty-four healthy kittens were randomly assigned to two groups (<i>n</i> = 12): the control group was provided a basal diet and the probiotics group was provided the basal diet supplemented with complex probiotics (given at the same time daily). The kittens were acclimatized for 5 days, and the experiment was conducted for 14 days. We collected feces from each kitten on days 1 and 14 for metabolomic analyses. Compared to the control, the probiotics group had significantly higher (<i>p</i> < 0.05) methylmalonylcarnitine, lysyl-hydroxyproline, phenylpropionylglycine, and vitamin K3 levels, and significantly lower (<i>p</i> < 0.05) gamma-glutamyl-L-putrescine, cis-gondoic acid, myristic acid, 12,13-DiHOME, and glycodeoxycholic acid levels. The results of this study suggest that complex probiotics promote intestinal health in kittens by regulating changes in various metabolites in the intestine and may have a mitigating effect on intestinal inflammation.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11758643/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143036001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Low Inclusion Rate of Olive Cake in Dairy Cow Rations on Uterine Health and Fertility Indices During Early Lactation.","authors":"Ioannis Nanas, Themistoklis Giannoulis, Eleni Dovolou, Ilias Giannenas, Georgios S Amiridis","doi":"10.3390/ani15020269","DOIUrl":"10.3390/ani15020269","url":null,"abstract":"<p><p>Olive cake was incorporated at a low inclusion rate (3.7%) into the rations of dairy cows through partial substitution of maize, and its effects on milk production, general health, and fertility traits were investigated. Multiparous purebred Holstein dairy cows (n = 148) were divided into two groups: a treated group (n = 86) and a control group (n = 62). The control ration (group C) was modified by replacing 1 kg of maize with an equal quantity of olive cake (group T). The experiment lasted from 60 days prepartum to 150 days postpartum. Electronic aids were utilized to quantify daily milk yield and detect estrus expression, while clinical and uterine examinations were performed weekly. Blood samples, uterine epithelial cells, and endometrial tissue samples were collected from a subgroup of healthy animals in both groups at specific time points. Blood samples were examined to determine the β-hydroxybutyric acid (BHBA), acute phase proteins (haptoglobin [Hpt] and serum amyloid A [SAA]), progesterone, and pregnancy-associated glycoproteins concentrations. Uterine epithelial cells were assessed for polymorphonuclear neutrophil (PMN) counts, and the expression of nine genes encoding inflammatory cytokines and immune system activation was analyzed in uterine biopsy tissue. No significant differences (<i>p</i> > 0.05) were observed between groups in milk yield, general morbidity, clinical endometritis, or conception rates. However, animals in group T came to estrus approximately 6 days earlier (<i>p</i> = 0.013) than those in group C; progesterone concentrations on day 7 of the subsequent cycle tended (<i>p</i> = 0.07) to be higher in group T. On day 21, BHBA concentrations were higher in group C than in group T (<i>p</i> < 0.05). Throughout the experiment, Hpt levels in group T were consistently lower (<i>p</i> < 0.001), while SAA was lower on day 7 compared to group C. From days 21 to 42 postpartum, there was a significant reduction in PMN numbers (group C <i>p</i> = 0.02; group T <i>p</i> < 0.0001), with a tendency for a greater reduction rate in group T (<i>p</i> = 0.08). Among the genes studied, a significant difference was revealed in the expression of the ILA1 gene, with strong correlations of gene expression in group C and weak to moderate correlations in group T. In conclusion, under the conditions of this experiment, the inclusion of olive cake into dairy cows' rations did not affect milk production but improved certain health and fertility parameters, making olive cake a suitable alternative feedstuff for high-producing animals.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11758664/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143036095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the Protective Effects of MitoQ and Antifreeze Protein III on Cryopreserved Canine Sperm.","authors":"Abbas Farshad, Emilia Diel, Axel Wehrend","doi":"10.3390/ani15020270","DOIUrl":"10.3390/ani15020270","url":null,"abstract":"<p><p>Cryopreservation can adversely affect sperm motility, structural integrity, and fertilization ability. This study investigated the effects of MitoQ and antifreeze protein III (AFP III) on frozen-thawed semen from eight adult dogs using a Tris-fructose extender. Ejaculates were divided and diluted with a standard Tris-fructose-egg yolk extender containing MitoQ (200 nM/mL) and AFP III (0.75, 1.0, 2.0 µg/mL), individually or combined. Post-thaw, samples were evaluated for motility, viability, membrane and acrosome integrity, lipid peroxidation, apoptosis indicators, mitochondrial function, and reactive oxygen species (ROS-H₂O₂). The results showed significant (<i>p</i> < 0.05) improvements in motility rate, progressive motility, VAP, VSL, VCL, ALH, and BCF with MitoQ or AFP alone. AFP III (0.75, 1.0 µg/mL) showed higher values than controls (<i>p</i> > 0.05), while MitoQ alone showed no significant effect. Viability and acrosome integrity improved with AFP III. Membrane integrity and lipid peroxidation were better in 0.75 and 1.0 µg/mL AFP III groups. ROS-H₂O₂ levels and mitochondrial membrane potential were unaffected except at 1.0 µg/mL AFP III. The phosphatidylserine translocation assay showed no significant differences in dead sperm between controls and individual treatments, but significant differences occurred with combined MitoQ/AFP III. In conclusion, AFP III and MitoQ in diluents protect canine sperm cells from cryodamage.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11763333/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Replacing Inorganic Sources of Copper, Manganese, and Zinc with Different Organic Forms on Mineral Status, Immune Biomarkers, and Lameness of Lactating Cows.","authors":"Manqian Cha, Xingjun Ma, Yunlong Liu, Shengyang Xu, Qiyu Diao, Yan Tu","doi":"10.3390/ani15020271","DOIUrl":"10.3390/ani15020271","url":null,"abstract":"<p><p>(Objectives) The objectives of this study were to evaluate the effect of half-replacement of the supplementary sulfate sources of Cu, Mn, and Zn with methionine-hydroxy-analog-chelated (MHAC) mineral or amino-acid-complexed (AAC) mineral forms in diets on the mineral status, blood immune biomarkers, and lameness of lactating cows. (Methods) Sixty multiparous Holstein cows (158 ± 26 days in milk; body weight: 665 ± 52 kg; milk yield: 32 ± 7 kg/day) were randomly assigned into one of three dietary treatments (<i>n</i> = 20 per group): (1) MHAC: 50% replacement of sulfate minerals with MHAC forms. (2) AAC: 50% replacement of sulfate minerals with AAC forms. (3) S: 100% sulfate minerals (control). Their Cu, Mn, and Zn concentrations, blood immune biomarkers, and lameness were measured monthly. Repeated-measure mixed models were used to evaluate the effects on trace mineral status over time. As the responses with the MHAC and AAC forms were similar, the treatments were also analyzed as organic trace minerals (OTMs, combining the MHAC and AAC groups, <i>n</i> = 40) versus inorganic trace minerals (ITMs, the S group, <i>n</i> = 20). (Results) Cows supplemented with OTMs had higher concentrations of Cu and Mn in their serum (<i>p</i> ≤ 0.05), a higher hoof hardness (<i>p</i> ≤ 0.05), and a lower incidence of lameness compared to those with ITMs on d 90. There were no statistical differences (<i>p</i> > 0.10) in the concentrations of IgA, IgG, or ceruloplasmin, but there were significant differences (<i>p</i> = 0.03) in the concentrations of IgM in the serum as fixed effects of the diet treatments during the whole trial. On d 30 and 90, the serum IgA concentrations of the cows supplemented with OTMs tended to be higher (0.05 < <i>p</i> ≤ 0.10) than those in the cows supplemented with ITMs. (Conclusions) The half-replacement strategy showed that the MHAC and AAC sources of Cu, Mn, and Zn additives had similar effects on the production performance, blood immune biomarkers, and lameness of the lactating cows. The long-term replacement strategy with OTMs led to the enhancement of the trace mineral concentrations in their body fluids, blood immune biomarkers, and hoof health.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11763283/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143036042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Dietary Iron Levels on the Production Performance, Nutrient Digestibility, Blood Biochemistry, and Meat and Fur Quality of Growing Rex Rabbits.","authors":"Jiali Chen, Jiyuan Wang, Lei Liu, Fuchang Li","doi":"10.3390/ani15020274","DOIUrl":"10.3390/ani15020274","url":null,"abstract":"<p><p>The objective of this study was to explore the effects of dietary iron (Fe) levels on the production performance, nutrient digestibility, blood biochemistry, and meat and fur quality of growing Rex rabbits. Two hundred 3-month-old Rex rabbits were randomly allocated to five groups, each with forty replicates. Rabbits were fed a basal diet supplemented with varying levels of Fe (0, 20, 40, 80, and 160 mg/kg) in the form of ferrous sulfate monohydrate. The analyzed Fe concentrations in the diets were 8.2, 25.4, 49.1, 85.6, and 178.7 mg/kg, respectively. Over a 35-day trial period, rabbits supplemented with 40 mg/kg Fe showed a markedly increased average daily feed intake, average daily gain, and fur area compared to the control group (<i>p</i> < 0.05). The addition of 20 and 40 mg/kg Fe markedly improved the digestibility of crude protein and nitrogen (<i>p</i> < 0.05). Additionally, 80 mg/kg Fe supplementation significantly increased the redness of the <i>longissimus dorsi</i> muscle 30 min postmortem (<i>p</i> < 0.05). Dietary Fe addition also significantly elevated serum concentrations of Fe and copper (<i>p</i> < 0.05) while decreasing the total Fe-binding capacity (<i>p</i> < 0.05). In conclusion, dietary Fe supplementation boosted growth performance, protein and nitrogen digestibility, Fe stores, and meat and fur quality in Rex rabbits. Specifically, 40 mg/kg Fe (diet Fe content of 49.1 mg/kg) improved growth performance, nutrient digestibility, and fur quality, while 80 mg/kg Fe (diet Fe content at 85.6 mg/kg) was optimal for enhancing meat quality.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11759148/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Prospective Study of the Prevalence and Predictive Risk Factors of Repeat Breeder Syndrome in Dairy Cattle in the North of Spain.","authors":"Sofía L Villar, Carlos C Pérez-Marín, Jacobo Álvarez, Antía Acción, Renato Barrionuevo, Juan J Becerra, Ana I Peña, Pedro G Herradón, Luis A Quintela, Uxía Yáñez","doi":"10.3390/ani15020266","DOIUrl":"10.3390/ani15020266","url":null,"abstract":"<p><p>This study examined the prevalence and risk factors of repeat breeder syndrome (RB) in 2370 dairy cows in northern Spain. Data collected included the prevalence of postpartum pathologies, metabolic markers, and productive and reproductive parameters. The overall RB prevalence was 21.1%. In primiparous cows, the postpartum body condition loss (Odds Ratio (OR): 2.9-8.1, depending on severity), ketosis (OR: 3.5), lameness (OR: 3.4), clinical mastitis (OR: 4.2), dystocia (OR: 5.2), and endometritis (OR: 12.2) significantly increased the RB risk. Summer (OR: 0.4) or autumn (OR: 0.6) calvings reduced this likelihood compared to winter or spring calvings. In multiparous cows, risk factors included body condition loss (OR: 2.0), ketosis (OR: 4.3), lameness (OR: 2.5), clinical mastitis (OR: 4.2), dystocia (OR: 1.9), endometritis (OR: 6.2), metritis (OR: 1.38), and subclinical mastitis (OR: 1.9). A longer interval between calving and first postpartum insemination reduced the RB risk (OR: 0.99). Overall, metabolic and reproductive disorders were significant risk factors for RB across both cow groups, while seasonal calving and delayed insemination mitigated risk in primiparous and multiparous cows, respectively.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11762659/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}