AnimalsPub Date : 2025-03-19DOI: 10.3390/ani15060878
Clive J C Phillips
{"title":"Developments in Chinese Attitudes to Animal Welfare.","authors":"Clive J C Phillips","doi":"10.3390/ani15060878","DOIUrl":"10.3390/ani15060878","url":null,"abstract":"<p><p>China has strong traditions of respect for animals, but momentous changes in recent history have changed society dramatically, and it cannot be assumed that animal welfare is always good. Farm animal production is being intensified rapidly, which can reduce resources offered to animals, in particular space for comfortable living. However, amongst workers in the animal industries, there is little optimism about their ability or need to improve animal welfare. Nevertheless, there is growing attention to animal welfare science in China, which is focused on remote sensing and technological approaches. The Chinese government currently has relatively little involvement in supporting good animal welfare standards, although legislation is in place. Attitudes are influenced by many factors, including the law, age, experience, occupation, and gender. The relatively strong involvement of women in caring for farm animals is encouraging as they tend to have more empathetic attitudes toward animals. It is concluded that attitudes are changing rapidly, but so are animal production systems, which make the development and improvement of animal welfare on farms especially important.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 6","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11939795/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143727554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
AnimalsPub Date : 2025-03-19DOI: 10.3390/ani15060880
Marek Gaworski
{"title":"How Do We Recognize the Effects of Dairy Cattle Activity in the Lying Area? A Case Study in Free-Stall Barns.","authors":"Marek Gaworski","doi":"10.3390/ani15060880","DOIUrl":"10.3390/ani15060880","url":null,"abstract":"<p><p>Dairy cattle kept in barns with a free-stall system have contact with the structural elements of the stalls in the lying area. As a result of the cattle entering the lying stall, lying down, and standing up, shiny surfaces appear on the neck rails and partitions. These shiny surfaces can be a source of information about the activity of the animals in the lying stalls over a long period of time. This case study aimed to assess the diversity of shiny spots on the neck rails and partitions in the lying area of barns with a free-stall system. The length of the shiny surfaces and their locations on the neck rails and partitions were measured in three barns (on one dairy farm) containing a total of 512 lying stalls. The differences in the lengths of the shiny surfaces on the left and right partitions were assessed, and their locations on the partitions and neck rail were compared. Identifying the interactions between animals and metal elements of the lying stalls could contribute to improving the welfare of dairy cattle.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 6","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11939605/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143727706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"CTNNB1 and CDH1 Regulate Trophoblast Cell Adhesion and Junction Formation in Yak Placental Tissue at Different Gestational Stages.","authors":"Bohao Zhang, Chen Song, Bin Zhou, Junjun Zhang, Weitao Dong, Yong Zhang, Xingxu Zhao, Quanwei Zhang","doi":"10.3390/ani15060876","DOIUrl":"10.3390/ani15060876","url":null,"abstract":"<p><p>Yaks (<i>Bos grunniens</i>), which are distributed across the Tibetan Plateau and other high-altitude regions, are vital livestock that provide essential resources for local herders and have significant economic and ecological value [...].</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 6","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11939409/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143727545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
AnimalsPub Date : 2025-03-19DOI: 10.3390/ani15060882
Jenna C P Wagner, Mark Edwards, Shweta Trivedi, Larry J Minter, Kimberly Ange-van Heugten
{"title":"Domestic Quarter Horse (<i>Equus caballus</i>) Milk Macronutrient Composition Analyses Within Micro Quantities from Two Different Geographical Locations.","authors":"Jenna C P Wagner, Mark Edwards, Shweta Trivedi, Larry J Minter, Kimberly Ange-van Heugten","doi":"10.3390/ani15060882","DOIUrl":"10.3390/ani15060882","url":null,"abstract":"<p><p>Microquantity laboratory procedures (<5 mL) are often used to analyze milk from non-bovid or less common species due to limited available samples and small sample sizes, unlike the large quantities of milk needed for macroquantity (~20 mL) assays standard in the commercial dairy industry. To better understand microquantity milk laboratory analyses, quarter horse (<i>Equus caballus</i>) milk from North Carolina State University (females = 4, n = 43) and California Polytechnic State University (females = 4, n = 42) equine centers was compared to investigate differences in macronutrient composition from 4 to 130 days after parturition. All mares were healthy, but consumed different diets. Major milk macronutrients were measured at the Smithsonian National Zoo and Conservation Biology Institute using a microquantity analysis of ash, crude protein (CP), dry matter (DM), crude fat, sugar, and gross energy. Of the six measured nutrients, only CP and crude fat differed (<i>p</i> < 0.05) by location, while sugar and crude fat had differences among individual mares. Californian mares had greater fat concentration means on a dry matter basis (DMB) than the North Carolinian mares (ANCOVA, <i>p</i> = 0.003). North Carolinian mares had greater CP concentration means than Californian mares. These findings indicate that dietary differences and/or environmental factors may play a role in CP and crude fat milk macronutrient composition within horse breeds. However, despite the differences noted, the microquantity analyses for nutritional means for all eight mares were within macroquantity horses ranges available in the previous literature. The results indicate that micro versus macroassays are comparable and supports clinical and scientific research on milk from species where large sample quantities are difficult to obtain.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 6","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11939680/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143727048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"New Data on <i>Rhinogobius chiengmaiensis</i> and <i>Rhinogobius mekongianus</i> in Thailand by DNA Barcoding and Morphological Methods.","authors":"Siriluck Tuncharoen, Paiboon Panase, Nontree Panprommin, Eakapol Wangkahart, Supranee Ruenkoed, Keatipong Mongkolwit, Dutrudi Panprommin","doi":"10.3390/ani15060871","DOIUrl":"10.3390/ani15060871","url":null,"abstract":"<p><p>A combination of morphological analysis and DNA barcoding (partial sequences of the cytochrome c oxidase I (COI) gene) was used to differentiate four gobiid fish species in the family Oxudercidae. <i>Rhinogobius chiengmaiensis</i> and <i>Rhinogobius mekongianus</i> were found in Thailand, while <i>Eugnathogobius siamensis</i> and <i>Pseudogobiopsis oligactis</i> were used for comparative purposes. Morphological identification relied on appearances, counts, and measurements. The 707-base pair COI sequences from eleven samples of four gobiid species were compared with reference sequences in public databases to confirm their scientific names. The average AT content was 51.8 ± 0.5% and the GC content was 48.2 ± 0.5%. Intraspecific genetic distances ranged from 0.00-0.28%, while interspecific genetic distances ranged from 0.86-16.63%. A neighbor-joining (NJ) phylogenetic tree depicted the relationships among the COI sequences of these species. Morphological analysis and COI sequences successfully distinguished the four gobiid species. Notably, the COI sequences of <i>R. chiengmaiensis</i>, <i>R. mekongianus</i>, and <i>E. siamensis</i> were previously unreported, hence, this study is the first report to add their sequences to public databases. These results can serve as valuable information for the management of aquatic resources, conservation, and aquaculture efforts.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 6","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11939654/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143727490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
AnimalsPub Date : 2025-03-19DOI: 10.3390/ani15060872
Edita Meškinytė, Vigilijus Jukna, Vilma Zigmantaitė, Oksana Ilina, Audrius Kučinskas
{"title":"The Effectiveness of the Use of Ultrasound Methodology (Applied to Live Animals) to Assess the Quality of Meat.","authors":"Edita Meškinytė, Vigilijus Jukna, Vilma Zigmantaitė, Oksana Ilina, Audrius Kučinskas","doi":"10.3390/ani15060872","DOIUrl":"10.3390/ani15060872","url":null,"abstract":"<p><p>The use of non-invasive technologies is among the most accurate methods for assessing meat quality parameters. Beef tenderness and palatability are strongly influenced by the amount of intramuscular fat, commonly referred to as marbling. Marbling is widely used in breeding programs to select animals with superior meat quality. In this study, we analyzed 236 Angus bulls and 22 Angus heifers from various farms in Lithuania, all aged 450 ± 112 days. Ultrasound examinations were performed using a linear transducer with a silicone standoff to evaluate the loin eye area. Key meat quality indicators, such as fat thickness, loin area and thickness, and intramuscular fat, were found to increase with the weight of bulls, ranging from 365 ± 12.70 kg to 825.57 ± 11.75 kg (<i>p</i> < 0.001), and heifers, ranging from 273 ± 20.71 kg to 767 ± 41.01 kg (<i>p</i> < 0.001). The highest intramuscular fat values were observed at 7.50 ± 0.75% (<i>p</i> < 0.05) to 6.9 ± 1.36% (<i>p</i> < 0.1) in bulls weighing 431.33-825.57 kg and 7.98 ± 0.28% (<i>p</i> < 0.001) in heifers weighing 603 kg. These findings demonstrate the feasibility of selecting Angus cattle for improved meat quality traits across different weight categories.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 6","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11939753/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143727605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
AnimalsPub Date : 2025-03-19DOI: 10.3390/ani15060874
Lorna Cameron, Meg Challinor, Sophie Armstrong, Abigale Kennedy, Sarah Hollister, Katharine Fletcher
{"title":"Tracking the Track: The Impact of Different Grazing Strategies on Managing Equine Obesity.","authors":"Lorna Cameron, Meg Challinor, Sophie Armstrong, Abigale Kennedy, Sarah Hollister, Katharine Fletcher","doi":"10.3390/ani15060874","DOIUrl":"10.3390/ani15060874","url":null,"abstract":"<p><p>Horses evolved to live in herds, continuously moving and foraging. Domestic horses often have limited social contact and movement, with high calorific intake, contributing to obesity. \"Track grazing\" is an increasingly popular management system, but evidence of its impact on weight management, behaviour, and welfare is limited. Horses (<i>n</i> = 19) resident at HorseWorld (UK) were observed July to October 2023, monitoring bodyweight (kg), Body Conditions Score (BCS), hoof health, behaviour, and welfare indicators. Horses on track grazing systems lost significantly more bodyweight (kg) (<i>F</i>(1,15) = 8.752, <i>p</i> = 0.010) than their restricted grazing counterparts, although this was not supported by BCS findings which may be ineffective for subtle body weight changes. Horses on track grazing systems lost (mean ± S.D.) 10.67 ± 9.9 kg from their starting weight, whereas those on the traditional paddock systems gained 6.8 ± 13.7 kg. Hoof health and general welfare assessment scores were maintained. More behaviours in \"positive\", \"negative\", and \"neutral\" categories were observed in paddock system horses than those on the track grazing system, although these differences were not statistically significant. These findings suggest that horses prone to bodyweight gain can be managed effectively using track grazing systems whilst maintaining health and welfare.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 6","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11939410/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143727617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
AnimalsPub Date : 2025-03-19DOI: 10.3390/ani15060875
Eniola Betiku, T Tiwa Ogundipe, Tanmaie Kalapala, Tomi Obe
{"title":"A Mini-Review on Multi-Hurdle Control of <i>Salmonella</i> Along Poultry Production Continuum.","authors":"Eniola Betiku, T Tiwa Ogundipe, Tanmaie Kalapala, Tomi Obe","doi":"10.3390/ani15060875","DOIUrl":"10.3390/ani15060875","url":null,"abstract":"<p><p>This mini-review presents common strategies for controlling <i>Salmonella</i> in poultry, addressing combined pre-harvest and post-harvest interventions to create a multi-hurdle approach. The goal is to highlight integrated approaches that enhance overall food safety and sustainability within the poultry industry. Current pre-harvest and post-harvest strategies are discussed, including industry practices and regulatory frameworks. Emphasis is placed on the implementation of biosecurity measures, vaccination, feed management, and environmental control in pre-harvest settings, as well as processing plant interventions such as antimicrobials for carcass decontamination, sanitation, and quality control measures. Pre-harvest strategies that have shown promise include enhanced biosecurity protocols, selective vaccinations for pathogenic <i>Salmonella</i> strains, and advanced feeding regimens. Post-harvest interventions, such as antimicrobial application for decontamination, have improved sanitation practices, and pathogen reduction technologies are also critical in reducing <i>Salmonella</i> prevalence. An integrated approach that combines both pre-harvest and post-harvest measures is essential for an effective <i>Salmonella</i> control program. Implementing a continuum of control strategies for <i>Salmonella</i> in poultry production is vital for ensuring food safety and protecting public health. Collaborative efforts between researchers, industry stakeholders, and policymakers are necessary to address emerging issues and enhance overall effectiveness.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 6","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11939138/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143727349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
AnimalsPub Date : 2025-03-19DOI: 10.3390/ani15060884
Federica Pesce, Emanuela Sannino, Enza Ragosta, Laura Marigliano, Giuseppe Picazio, Mauro Esposito, Maria Dimatteo, Barbara Degli Uberti, Susanna De Luca, Noemi Di Caprio, Domenico Citarella, Renato Pinto, Giovanna Fusco, Esterina De Carlo, Gianluca Miletti
{"title":"Overkilling in a Dog: A Case Report.","authors":"Federica Pesce, Emanuela Sannino, Enza Ragosta, Laura Marigliano, Giuseppe Picazio, Mauro Esposito, Maria Dimatteo, Barbara Degli Uberti, Susanna De Luca, Noemi Di Caprio, Domenico Citarella, Renato Pinto, Giovanna Fusco, Esterina De Carlo, Gianluca Miletti","doi":"10.3390/ani15060884","DOIUrl":"10.3390/ani15060884","url":null,"abstract":"<p><p>The term \"overkilling\" in forensic medicine is not clearly defined and is used to refer to homicides involving unusually massive injuries, far exceeding those necessary to kill the victim. This is the clinical case of a dog found in February 2023 in a town near Naples, with a rope around its neck and the metacarpal region of its forelimbs. The dog was taken to the Istituto Zooprofilattico Sperimentale del Mezzogiorno (IZSM, Portici, Southern Italy), where it underwent a total body radiographic study performed using the \"Philosophy HF400\" X-ray device (Pan Vet, Kildare Town, Ireland). Subsequently, a full autopsy was performed, and samples of the injured organs were analyzed by accredited in-house laboratories for microbiological, histological and toxicological analyses. The autopsy revealed anatomopathological lesions compatible with strangulation, which were confirmed by histological examination. The autopsy also reported serosanguineous chest effusion, food material mixed with blackish microgranules and harmful substances in the stomach and, finally, uncoagulated blood in the atrioventricular chambers of the heart. These findings raised the suspicion of poisoning, which was confirmed by the positive outcome of toxicological tests.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 6","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11939647/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143727571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
AnimalsPub Date : 2025-03-19DOI: 10.3390/ani15060873
Samuel Lemos, Luis Llaneza, Armando Pereira, Aurora Monzón
{"title":"Food Habits of the Wolf in a Low-Density Territory in the Northeast of Trás-os-Montes (Portugal).","authors":"Samuel Lemos, Luis Llaneza, Armando Pereira, Aurora Monzón","doi":"10.3390/ani15060873","DOIUrl":"10.3390/ani15060873","url":null,"abstract":"<p><p>The study of carnivores' diets is an important conservation tool, which can minimize conflicts with different stakeholders and provide proper substantiations for management measures. The main aim of this study was to understand the diet of a small, isolated pack named Mogadouro Sul, present in the Northeast of Trás-os-Montes (Portugal). Scat analysis was the method used to determine what wolves consumed. The specific origin of all collected fecal samples (n = 78) was confirmed by molecular analysis. The results, expressed in frequency of occurrence (FO) showed that domestic animals were the most frequent food category in the wolf's diet (78.3% FO), with a special incidence in goats (40.6% FO), although wild ungulates (roe deer and wild boar) also accounted for 21.7% FO of the diet. The study pack presented a diet diversity (H') of 0.65 and a food niche breadth (B') of 0.55. Food availability did not appear to be a limiting factor, and the wolf's presence in the study area could be explained by changes in land use and increased infrastructure. This trophic behavior may threaten the conservation of this pack due to the persecution it may face.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 6","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11939525/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143727678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}