Animals最新文献

{"title":"Tandem Administration of Prostaglandin F_2α and Gonadotropin-Releasing Hormone in Beef Heifers and Cows as a Convergent Presynchronization Method in the 7 & 7 Synch Protocol.","authors":"Lucas J Palcheff, Genevieve M VanWye, Kimberly R Ricardo, Kendal L Green, Franklin J Even, Samantha R Roberts, Adella B Lonas, Christine M Spinka, Scott E Poock, Saulo Menegatti Zoca, Jessica N Drum, Jordan M Thomas","doi":"10.3390/ani15091329","DOIUrl":"10.3390/ani15091329","url":null,"abstract":"<p><p>An experiment was designed to evaluate modifications to the 7 & 7 Synch protocol, with the hypothesis that tandem administration of prostaglandin F2α (PG) and gonadotropin-releasing hormone (GnRH) during the presynchronization portion of the protocol would generate a convergent ovarian response among anestrous and estrous cycling females. Nulliparous, primiparous, and multiparous Angus and Angus-cross females were blocked based on parity and pre-treatment luteal status and, within a block, randomly assigned to one of three treatments. The females assigned to the 7 & 7 Synch treatment (n = 765) received an intravaginal progesterone-releasing insert (CIDR^®) and an administration of PG on Day 0, GnRH on Day 7, and PG concurrent with CIDR removal on Day 14. Estrotect™ estrus detection aids were applied on Day 14 at the time of CIDR removal and PG administration. The females assigned to the 7 & 7 + G treatment (n = 769) received the same treatment schedule, with the modification that GnRH was administered in tandem with PG on Day 0. The females assigned to the 7 & 7 + G + P treatment (n = 786) received the same treatment schedule, with the modification that GnRH was administered in tandem with PG on both Days 0 and 7. Fixed-time artificial insemination (FTAI) was performed at 54 ± 2 h (nulliparous) or 66 ± 2 h (primiparous and multiparous) after CIDR removal. Transrectal ovarian ultrasonography was performed on Days 7 and 14 to characterize the largest follicle diameter (LFD) and the presence of corpora lutea (CL). Estrus prior to FTAI was characterized using estrus detection aids. Pregnancy status was determined via transrectal ultrasonography approximately 60-100 days after FTAI. The proportion of multiparous females with CL on Day 14 tended to be greater following treatment with 7 & 7 Synch or 7 & 7 + G (90% and 87%, respectively), as compared to females treated with 7 & 7 + G + P (82%; <i>p</i> = 0.09). A greater proportion of multiparous females expressed behavioral estrus prior to FTAI when treated with 7 & 7 + G + P (90%) as compared to multiparous females treated with 7 & 7 + G (79%; <i>p</i> < 0.002) or 7 & 7 Synch (78%; <i>p</i> = 0.0004); however, pregnancy rates resulting from FTAI did not differ based on treatment. In summary, tandem administration of GnRH and PG on Days 0 and 7 of the 7 & 7 Synch protocol effectively generated a convergent ovarian response among females with variable pretreatment luteal status but failed to enhance pregnancy rates to FTAI.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 9","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12070911/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143965344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Role of Black Soldier Fly Larvae in Optimizing Performance and Amino Acid Composition in Thai Native Chicken.","authors":"Theeraphat Srikha, Padsakorn Pootthachaya, Warin Puangsap, Suphakon Pramotchit, Wuttigrai Boonkum, Chanon Suntara, Yupa Hanboonsong, Anusorn Cherdthong, Bundit Tengjaroensakul, Sawitree Wongtangtintharn","doi":"10.3390/ani15091330","DOIUrl":"10.3390/ani15091330","url":null,"abstract":"<p><p>The objective of this study was to investigate the effects of black soldier fly larvae (BSFL) inclusion on the performance, economic return, carcass traits, meat quality, amino acid profile, and purine content in the breast meat of Thai native chickens (TNCs) (Pradu Hang Dam Mor Kor 55: PD) from 10 to 70 days of age. A total of <b>216 ten-day-old PD chickens (108 males and 108 females)</b> were randomly assigned to three dietary treatments with four replicates per treatment (18 birds per replicate, with a 1:1 male-to-female ratio). The treatments included (T1) control diet with soybean meal as the main protein source, (T2) 10% BSFL meal replacing soybean meal, and (T3) 12% BSFL meal replacing soybean meal. The results showed that BSFL inclusion had no significant effect on overall growth performance (<i>p</i> > 0.05), but it reduced abdominal fat and feed cost per gain while improving economic returns (<i>p</i> < 0.05). Additionally, BSFL increased some breast meat amino acid profiles, such as serine, glutamic acid, threonine, arginine, and lysine (<i>p</i> < 0.05), and increased total purine content (<i>p</i> < 0.05), although the values were still within the safe limit for human consumption. These results suggest that BSFL can be used as a sustainable alternative protein source in Thai native chicken diets without compromising product performance or quality.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 9","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12071139/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143971981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Health Status of Skopelos Goats and Its Impact on Milk Yield Under Intensive and Extensive Farming Systems.","authors":"Vera Korelidou, Aphrodite I Kalogianni, Georgios Arsenos, Athanasios I Gelasakis","doi":"10.3390/ani15091328","DOIUrl":"10.3390/ani15091328","url":null,"abstract":"<p><p>Health and welfare are fundamental to goat production; however, their impact on milk yield in goats reared under different farming systems remains insufficiently studied. The objective was to examine the frequency of various health issues in dairy goats reared under two different farming systems and to assess their effects on daily milk yield (DMY). A total of 286 Skopelos goats from an intensive (N_A = 153) and an extensive (N_B = 133) farm, all with the same genetic background, were prospectively monitored for two consecutive lactation periods. Each goat was clinically examined for animal-based health indicators every 50 days, while individual DMY was also recorded. Morbidity frequency measures (cumulative incidence, point, and period prevalence) were calculated for each one of the studied health issues, and mixed linear regression models were developed to assess the effect of each one of the studied health issues on DMY. The results indicated that overgrown hooves were more common in intensively reared goats, while anemia and nasal discharge prevailed in extensively reared goats. Udder asymmetry, udder fibrosis, body abscesses, poor hair coat quality, and swollen lymph nodes were common in both farms, albeit more prevalent in extensively reared goats, except for udder asymmetry. Among the studied health issues, lameness (18.5%, <i>p</i> < 0.05), mouth lesions (14.1%, <i>p</i> < 0.05), udder asymmetry (6.6%, <i>p</i> < 0.001), and udder fibrosis (9.5%, <i>p</i> < 0.001), were significantly associated with reductions in DMY. These findings provide valuable insights for optimizing herd health management practices in dairy goats based on evidence and farming system requirements.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 9","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12070935/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143971951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deep Learning-Based Classification of Canine Cataracts from Ocular B-Mode Ultrasound Images.","authors":"Sanghyeon Park, Seokmin Go, Seonhyo Kim, Jaeho Shim","doi":"10.3390/ani15091327","DOIUrl":"10.3390/ani15091327","url":null,"abstract":"<p><p>Cataracts are a prevalent cause of vision loss in dogs, and timely diagnosis is essential for effective treatment. This study aimed to develop and evaluate deep learning models to automatically classify canine cataracts from ocular ultrasound images. A dataset of 3155 ultrasound images (comprising 1329 No cataract, 614 Cortical, 1033 Mature, and 179 Hypermature cases) was used to train and validate four widely used deep learning models (AlexNet, EfficientNetB3, ResNet50, and DenseNet161). Data augmentation and normalization techniques were applied to address category imbalance. DenseNet161 demonstrated the best performance, achieving a test accuracy of 92.03% and an F1-score of 0.8744. The confusion matrix revealed that the model attained the highest accuracy for the No cataract category (99.0%), followed by Cortical (90.3%) and Mature (86.5%) cataracts, while Hypermature cataracts were classified with lower accuracy (78.6%). Receiver Operating Characteristic (ROC) curve analysis confirmed strong discriminative ability, with an area under the curve (AUC) of 0.99. Visual interpretation using Gradient-weighted Class Activation Mapping indicated that the model effectively focused on clinically relevant regions. This deep learning-based classification framework shows significant potential for assisting veterinarians in diagnosing cataracts, thereby improving clinical decision-making in veterinary ophthalmology.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 9","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12070956/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143973712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Gabapentin on the Efficiency of a Desensitization-Counter-Conditioning Claw-Trimming Protocol for Cats with Healthcare Phobias: A Double-Blind, Placebo-Controlled Crossover Trial.","authors":"Lua Raucourt, Sylvia Masson","doi":"10.3390/ani15091326","DOIUrl":"10.3390/ani15091326","url":null,"abstract":"<p><p>Feline healthcare phobia is a major challenge in veterinary practice, limiting medical care and increasing stress for cats, owners, and veterinarians. Traditional desensitization-counter-conditioning (DS-CC) protocols aim to reduce fear responses, but their success is limited in sensitized cats. This study evaluates whether gabapentin can improve the efficiency of DS-CC training in healthcare phobia in cats. Forty-two sensitized cats participated in a claw-trimming learning protocol. Using a double-blind, placebo-controlled crossover trial, the cats followed 10 training sessions: the first 5 under one product and the last 5 under the other one. Their progression through the learning steps was monitored. The results indicate that gabapentin significantly accelerated the cats' ability to complete DS-CC steps, suggesting a positive effect on the learning process. While some side effects, such as mild sedation and ataxia, were observed in 42% of the cats, they were transient and did not hinder participation. These findings suggest that gabapentin should be added when engaging patients in DS-CC protocols in order to lower the time needed to achieve therapy, which will improve the overall welfare of our patients. Further studies are needed to confirm these results in other types of therapies and in a clinical setting.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 9","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12070960/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143958282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Bacillus amyloliquefaciens</i> CU33 Fermented Product Improves Growth Performance, Diarrhea, and Immunity of Goat Kids.","authors":"Tsung-Yu Lee, Yueh-Sheng Lee, Chean-Ping Wu, Bor-Chun Weng, Kuo-Lung Chen","doi":"10.3390/ani15091324","DOIUrl":"10.3390/ani15091324","url":null,"abstract":"<p><p>This study focused on the physicochemical characteristics of the fermented products (FP) produced by <i>Bacillus amyloliquefaciens</i> CU33 (CU33) from soybean meal with 70% moisture. Additionally, it investigated the effects of adding FP to starter on the growth performance, general health performance, blood clinical biochemistry, and immunity of Alpine goat kids during the weaning period. Forty 14-day-old male Alpine goat kids were randomly assigned into starter supplementations of 0, 0.1, 0.3, or 0.5% CU33 FP for 8 weeks, and each goat kid was individually raised in stainless steel cage (width 70 cm × height 70 cm × depth 80 cm). The moisture after fermentation was linearly decreased as fermentation time increased (<i>p</i> < 0.05), and the pH value and <i>Bacillus</i>-like counts reached the highest at 24 h of fermentation. The activity of neutral protease and alkaline protease, the content of surfactin and γ-PGA, the viscosity, and the odor of CU33 FP were linearly increased as fermentation time increased (<i>p</i> < 0.05). The neutral protease activity, surfactin, γ-PGA, and viscosity increased after drying, whereas the moisture, pH value, <i>Bacillus</i>-like counts, and odor decreased (<i>p</i> < 0.05). During the pre-weaning period (0-4 weeks), the body weight gain (BWG) of the 0.1% CU33 FP group was higher than that of the control group (<i>p</i> < 0.05), and all CU33 FP groups showed a better feed conversion ratio (FCR) than the control group (<i>p</i> < 0.05). During the post-weaning period (4-8 weeks) and throughout the entire experimental period (0-8 weeks), the BWG and FCR of all CU33 FP groups were better than those of the control group (<i>p</i> < 0.05). Furthermore, both BWG and FCR improved linearly as the dietary level of CU33 FP increased (<i>p</i> < 0.05). Simultaneously, the fecal consistency index at 0-4 and 4-8 weeks and the coliform counts in the rectum at 4 weeks linearly decreased (<i>p</i> < 0.05), and the <i>Bacillus</i>-like counts in the rectum linearly increased at 4 and 8 weeks (<i>p</i> < 0.05). Phosphorous (P), total protein (TP), blood urea nitrogen (BUN) in serum at 8 weeks, and the oxidative burst capacity at 4 weeks linearly increased as the dietary level of CU33 FP increased, but the skin sensitization test showed a quadratic curve, and the 0.1% CU33 FP group had the lowest performance (<i>p</i> < 0.05). In conclusion, dietary supplementation with 0.1% of CU33 FP can improve the growth performance, diarrhea status, and oxidative burst capacity of Alpine goat kids, showing the potential to be a feed additive.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 9","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12071083/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143961834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Comparison of Artificial Intelligence and Human Observation in the Assessment of Cattle Handling and Slaughter.","authors":"Lily Edwards-Callaway, Huey Yi Loh, Carina Kautsky, Paxton Sullivan","doi":"10.3390/ani15091325","DOIUrl":"10.3390/ani15091325","url":null,"abstract":"<p><p>Slaughter facilities use a variety of tools to evaluate animal handling, including but not limited to live audits, the use of remote video auditing, and some AI technologies. The objective of this study was to determine the similarity between AI and human evaluator assessments of critical cattle handling outcomes in a slaughter plant. One hundred twelve video clips of cattle handling and stunning from a slaughter plant in the United Kingdom were collected. The AI identified the presence or absence of: Stunning, Electric Prod Usage, Falling, Pen Crowding, and Questionable Handling Events. Three human evaluators scored the videos for these outcomes. Four different datasets were generated, and Jaccard similarity indices were generated. There was high similarity (JI > 0.90) for Stunning, Electric Prod Usage, and Falls between the evaluators and the AI. There was high consistency (JI > 0.80) for Pen Crowding. There were differences (JI ≥ 0.50) between the humans and the AI when identifying Questionable Animal Handling Events but the AI was adept at identifying events for further review. The implementation of AI to assist with cattle handling in a slaughter facility environment could be an added tool to enhance animal welfare programs.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 9","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12071094/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143974315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparing the Use of Transverse Abdominis Plane Block and Splash Block for Postoperative Pain Control in Dogs Undergoing Mastectomy-A Blinded Randomized Prospective Clinical Study.","authors":"Daniele Corona, Simone K Ringer, Stefanie Keller, Iris M Reichler, Regula Bettschart-Wolfensberger, Annette P N Kutter","doi":"10.3390/ani15091323","DOIUrl":"10.3390/ani15091323","url":null,"abstract":"<p><p>To compare the use of postoperative analgesia for mastectomy, 44 dogs were randomly allocated to either the Splash treatment group (group A) or the Transverse Abdominis Plane block treatment group (TAP, group B). Following intramuscular (IM) premedication with pethidine (4 mg kg^-1) and acepromazine (0.01 mg kg^-1), anesthesia was induced with intravenous (IV) propofol and maintained with isoflurane by an anesthetist (DC) who was unaware of the treatment. In group A, ropivacaine 0.5% (2 mg kg^-1) was administered prior to surgical wound closure. In group B, ropivacaine 0.5% (0.8-1 mg kg^-1 per point) was administered by ultrasound-guided TAP block with two injection points per treated body side. At the end of the surgery, all dogs received pethidine (4 mg kg^-1 IM), meloxicam (0.2 mg kg^-1 IV), and acepromazine (0.005 mg kg^-1 IV). The animals' pain was assessed by the anesthetist, who remained unaware of the treatment type used, via the Short Form of the Glasgow Composite Pain Scale. When the pain scores were ≥6, methadone (0.2 mg kg^-1 IV) and gabapentin (10 mg kg^-1 per oral) were started. When the pain score remained ≥ 6, ketamine (1 mg kg^-1 subcutaneously) was administered. The dogs in the TAP block group had lower postoperative pain scores 3-12 h after anesthesia administration was terminated and required significantly less rescue analgesia.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 9","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12071095/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143965964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Impact of Adding Trehalose to the Diet on Egg Quality and Tibia Strength in Light-Laying Hens.","authors":"Fernando Perazzo Costa, Isabelle Kaneko, Thamires Ferreira, Jorge Muniz, Eliane Silva, Adiel Lima, Raul Lima Neto, Matheus Ramalho Lima, Thiago Moreira","doi":"10.3390/ani15091318","DOIUrl":"10.3390/ani15091318","url":null,"abstract":"<p><p>Trehalose, a disaccharide consisting of two D-glucose molecules, is present in a variety of organisms, including bacteria, yeast, fungi, insects, and plants. In plants, it functions as a source of energy and carbon, and in yeast and plants, it serves as a signaling molecule, influencing metabolic pathways and growth regulation. Additionally, it plays a role in protecting proteins and cell membranes from stress-induced damage. This study aims to assess the optimal level of trehalose supplementation in the diets of layer hens aged 34 to 49 weeks, addressing the limited existing literature on its effects on productivity. Experimental diets, designed in accordance with nutritional recommendations, were formulated to contain six different levels of trehalose (0, 0.05, 0.10, 0.30, 0.60, and 1.00%). The study was conducted over five 21-day periods, during which various performance parameters were evaluated. The results indicated that trehalose supplementation at levels of 0.05%, 0.10%, and 0.30% led to increased feed intake (FI) compared to the 1.00% level (<i>p</i> < 0.05). Furthermore, the highest trehalose level (1.00%) significantly reduced the feed conversion ratio by egg mass (FCRem) compared to both the control group and the other supplementation levels; however, the feed conversion ratio by dry matter (FCRDz) remained consistent across all treatments. The levels of 0.05%, 0.10%, and 0.30% exhibited superior FCREm and FCRDz compared to the 1.00% level. Egg weight (EW) was higher in the trehalose-supplemented groups compared to the control group. Additionally, the 1.00% trehalose treatment was found to be the most effective in terms of relative weights of shells (RWS), and egg mass (EM) was higher at all trehalose levels compared to the control group. The antioxidant status, as measured by malondialdehyde (MDA) levels, indicated that supplementation with 0.30% and 0.60% trehalose had a protective effect against oxidative stress, although the 1.00% level resulted in increased MDA levels. Total weight (TW) was highest in the 0.30% treatment group, and bone strength (BS) improved in the groups supplemented with 0.10% and 1.00% trehalose. Other parameters, including lipid content (L), dry matter (DM), phosphorus (P), and calcium (Ca), did not show any significant differences among the treatment groups. In conclusion, supplementation with 1.00% trehalose enhances feed efficiency, egg weight, and quality, with minimal impact on lipid peroxidation, while potentially providing benefits for gut health and egg quality.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 9","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12070847/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights into the Genetic Connectivity and Climate-Driven Northward Range Expansion of <i>Turbo sazae</i> (Gastropoda: Turbinidae) Along the Eastern Coast of Korea.","authors":"Young-Ghan Cho, Kyungman Kwon, Hyun Soo Rho, Won-Gi Min, Hee-Do Jeung, Un-Ki Hwang, Yong-Kyun Ryu, Areumi Park, Hyun-Ki Hong, Jong-Seop Shin, Hyun-Sung Yang","doi":"10.3390/ani15091321","DOIUrl":"10.3390/ani15091321","url":null,"abstract":"<p><p><i>Turbo sazae</i>, a commercially and ecologically significant marine gastropod traditionally found in Jeju Island and the southern coast of Korea, is experiencing a reported northward expansion into the East Sea, likely influenced by rising seawater temperatures. This study provides preliminary genetic insights into the genetic structure and connectivity of <i>T. sazae</i> populations between Jeju and the East Sea using mitochondrial COI sequences. Samples from 6 geographically distinct locations were analyzed, with three cloned replicates generated to enhance sequence reliability. Genetic diversity, haplotype distribution, and population differentiation were then assessed. Our analysis reveals potential genetic connectivity between Jeju and East Sea populations, possibly driven by larval dispersal via the Kuroshio and Tsushima Currents, highlighted by the predominance of shared haplotype EJ1 (60.0% in Jeju, 50.0% in East Sea). Bayesian phylogenetic analysis estimated the time to the most recent common ancestor (MRCA) between Jeju and East Sea populations at approximately 9.7 to 23.3 million years ago, indicating ancient divergence rather than very recent separation. Pairwise FST values and AMOVA results showed generally low levels of genetic differentiation. Given the small sample sizes and use of a single mitochondrial marker, these findings should be interpreted cautiously as preliminary evidence. Nevertheless, this study highlights the need for continued genetic monitoring of <i>T. sazae</i> populations under climate-driven range shifts and provides a foundation for future research incorporating broader genomic approaches.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 9","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12070958/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}