AnimalsPub Date : 2025-06-03DOI: 10.3390/ani15111648
Chao Fan, Huiliang Xue, Jinhui Xu, Ming Wu, Lei Chen, Laixiang Xu
{"title":"Two Species of Long-Day Breeding Hamsters Exhibit Distinct Gut Microbial Responses to Photoperiodic Variations.","authors":"Chao Fan, Huiliang Xue, Jinhui Xu, Ming Wu, Lei Chen, Laixiang Xu","doi":"10.3390/ani15111648","DOIUrl":"10.3390/ani15111648","url":null,"abstract":"<p><p>The relationship between the gut microbiota and photoperiod has received widespread attention, and it is necessary to explore the probable common mechanisms involved. We tested whether the gut microbiota of animals with similar light-regulated life history traits would also exhibit consistent responses to the photoperiod. Here, two species of long-day breeders, striped hamsters (<i>Cricetulus barabensis</i>) and Djungarian hamsters (<i>Phodopus sungorus</i>), were raised under different photoperiods (long daylight, LD; short daylight, SD), and their cecal contents were collected to assess the gut microbiota. There was no difference in the gut microbial diversity between the groups of striped hamsters; however, in the Djungarian hamsters, lower Chao and Shannon indices were observed in the LD group than in the SD group. The bacterial community variation in the striped hamsters was reflected mainly in the enrichment of the genera <i>Enterorhabdus</i> and <i>Jeotgalicoccus</i> in the LD group; meanwhile, more taxa with significant changes in relative abundance under different photoperiods were found in the Djungarian hamsters, such as the enrichment of the genera <i>Lactobacillus</i> and <i>Faecalibaculum</i> in the LD group and the enrichment of the genera <i>Ruminococcus</i> and <i>Colidextribacter</i> in the SD group. The LD conditions substantially reduced the complexity of the gut microbial network in the Djungarian hamsters and increased the <i>R</i><sup>2</sup> value of the striped hamster gut microbiota under fitting with a neutral community model. Moreover, the potential gut microbial functions in the striped hamsters were relatively stable, but variations were observed in multiple pathways between the groups of Djungarian hamsters. These results contribute to the understanding of host species specificity in the response of the gut microbiota to external changes.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 11","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12153784/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144282220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
AnimalsPub Date : 2025-06-03DOI: 10.3390/ani15111639
Nikolaus Velich, Britta Vidoni, Eberhard Ludewig, Alexander Tichy, Eva Schnabl-Feichter
{"title":"Comparative Radiographic Analysis of Trochleoplasties for Patellar Luxation Correction: Inter-Observer Agreement of a Modified Osteoarthritis Scoring System.","authors":"Nikolaus Velich, Britta Vidoni, Eberhard Ludewig, Alexander Tichy, Eva Schnabl-Feichter","doi":"10.3390/ani15111639","DOIUrl":"10.3390/ani15111639","url":null,"abstract":"<p><p>Patellar luxation is common in small breed dogs and is often treated surgically. This study compares the long-term outcomes of two surgical techniques, trochlear wedge recession (TWR) and trochlear block recession (TBR), for medial patellar luxation (MPL) with regard to osteoarthritis (OA) progression. High inter-observer agreement was hypothesized for the use of a modified OA scoring system and fewer OA changes in the TBR group. This study included 25 dogs (<15 kg) with grade-2 or -3 MPLs treated at the University of Veterinary Medicine Vienna (2016-2021). A total of 32 stifle joints (TWR Group n = 11, TBR Group n = 21) were evaluated pre-operatively and at least one year post-operatively using a modified OA scoring system. A statistical analysis was conducted to compare OA progression between the techniques. The inter-observer agreement was high. The OA scores increased in both groups post-operatively, with TBR demonstrating a greater progression at specific points. Overall, no significant differences were found between the techniques. These findings suggest that OA progression does not significantly differ between TWR and TBR. The modified OA scoring system has shown to be reliable for assessing OA progression after treatment. Further prospective studies with larger study populations incorporating clinical assessments are needed for a better understanding of the surgical impacts on OA development.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 11","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12153589/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144282065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
AnimalsPub Date : 2025-06-03DOI: 10.3390/ani15111643
Haerin Rhim, Maria G Aguilar, Kimberly L Boykin, Kaylie Zapanta, Janina A Krumbeck, Mark A Mitchell
{"title":"A Preliminary Investigation of the Gastrointestinal Bacterial Microbiomes of Barred Owls (<i>Strix varia</i>) Admitted to a Wildlife Hospital.","authors":"Haerin Rhim, Maria G Aguilar, Kimberly L Boykin, Kaylie Zapanta, Janina A Krumbeck, Mark A Mitchell","doi":"10.3390/ani15111643","DOIUrl":"10.3390/ani15111643","url":null,"abstract":"<p><p>Research on the gut microbiome, which includes microbial communities and genetic material in the gastrointestinal tract, has revealed essential roles beyond digestion, such as immune regulation, metabolism, and homeostasis. However, studies on birds-key ecosystem members-remain limited. Injured wild birds admitted to wildlife hospitals often receive antibiotics that can alter gut microbiota, leading to dysbiosis and promoting antimicrobial-resistant (AMR) bacteria. This study examined how hospitalization and antibiotics influence the cloacal microbiota of barred owls admitted for fracture repair. A total of 17 cloacal swab samples were analyzed using next-generation sequencing targeting 16S rRNA and AMR genes. Across all samples, Bacillota (Firmicutes), Actinomycetota (Actinobacteria), and Pseudomonadota (Proteobacteria) were the most abundant phyla. In non-antibiotic-treated owls, alpha and beta diversity showed no significant changes between admission and release; however, antibiotic-treated owls exhibited significant diversity shifts in these parameters at release. AMR genes were detected in most samples at admission, with some increasing significantly during hospitalization, suggesting an impact of antibiotic exposure. These findings provide insights into how antibiotics used in wildlife rehabilitation affect host microbiota and contribute to AMR gene dissemination.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 11","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12153569/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144282041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
AnimalsPub Date : 2025-06-03DOI: 10.3390/ani15111651
Mariska Barten, Yvette de Geus, Joop den Hartog, Len Lipman
{"title":"Decision-Making Regarding On-Farm Culling Methods for Dairy Cows Related to Cow Welfare, Sustainable Beef Production, and Farm Economics.","authors":"Mariska Barten, Yvette de Geus, Joop den Hartog, Len Lipman","doi":"10.3390/ani15111651","DOIUrl":"10.3390/ani15111651","url":null,"abstract":"<p><p>In the Netherlands, around 52,000 dairy cows die on the primary farm each year due to natural death, euthanasia, or on-farm emergency slaughter (OFES). The decision as to what is the best option is made by the farmer, often after consulting a veterinarian, a livestock trader, or a slaughterhouse operator. To determine which factors play a role in this decision-making process, semi-structured interviews were conducted with dairy farmers, private veterinary practitioners, livestock traders, and slaughterhouse operators in the Netherlands. Dairy cattle culling decisions are influenced and limited by strict enforcement of livestock transport regulations and limited options for on-farm killing methods. Requirements regarding mortality rates imposed by the dairy industry and private quality labels for raw milk also influence culling decisions in the Netherlands. Most participants stated that restrictive conditions regarding OFES and mobile slaughterhouses (MSHs) appear to have (unintended) negative effects on cow welfare and meat salvage in general. Different interests, such as cow welfare, food safety, economic concerns of various stakeholders, the reputational interests of the dairy and beef industries, and sustainability objectives such as meat salvage can be conflictive. The results of this study show that the decision-making process regarding culling or (prolonged) veterinary treatment of dairy cattle is complex because various factors, interests, and uncertainties must be weighed. This weighing can vary between individual dairy farms and individual dairy farmers.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 11","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12153892/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144282083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
AnimalsPub Date : 2025-06-03DOI: 10.3390/ani15111641
Tiarna Scerri, Sabrina Lomax, Peter Thomson, Benjamin Kimble, Peter White, Merran Govendir, Cameron Clark, Dominique Van der Saag
{"title":"Medicated Meloxicam Pellets Reduce Some Indicators of Pain in Disbudded Dairy Calves.","authors":"Tiarna Scerri, Sabrina Lomax, Peter Thomson, Benjamin Kimble, Peter White, Merran Govendir, Cameron Clark, Dominique Van der Saag","doi":"10.3390/ani15111641","DOIUrl":"10.3390/ani15111641","url":null,"abstract":"<p><p>Disbudding is a husbandry practice that causes pain and discomfort to calves. As a prominent welfare concern, it is now standard practice for calves to be given analgesic treatment such as a nonsteroidal anti-inflammatory drug (NSAID) injection. Meloxicam is a commonly used NSAID as it provides pain relief for up to 44 h following disbudding. However, since symptoms can persist for up to two weeks, it was hypothesised that more prolonged analgesic treatment would promote better welfare outcomes than the conventional injection. This study tested a novel treatment whereby disbudded calves were fed grain-based pellets medicated with meloxicam over a 7-day period. Lower levels of horn site inflammation were observed for the pellet treatment across the 7-day feeding period in comparison to the conventional injection. The pellet treatment calves also exhibited less pain-specific and more positive social-specific behaviours during and beyond the feeding period. Together, these results suggest that lower levels of inflammation enacted by prolonged meloxicam administration have an active role in reducing pain and maintaining the affectivity of disbudded calves. With the goal of establishing sustained disbudding treatment as a new industry standard, future research will focus on larger-scale results reproducibility and maximising treatment practicality.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 11","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12153791/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144282150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
AnimalsPub Date : 2025-06-03DOI: 10.3390/ani15111642
Nobuo Masataka
{"title":"Possible Anxiolytic Effects of Cannabidiol (CBD) Administration on Feline Responses to a Fear Response Test.","authors":"Nobuo Masataka","doi":"10.3390/ani15111642","DOIUrl":"10.3390/ani15111642","url":null,"abstract":"<p><p>In humans, cannabidiol (CBD), the primary non-addictive component of cannabis, is known to possess considerable therapeutic potential. The purpose of this study was to investigate the effects of CBD administration on reducing sound-induced fear in healthy domestic cats in a laboratory model of thunderstorm simulation. A total of 40 cats, each naïve to the current testing, were randomly assigned into either of two administration groups (CBD and placebo). Each group was then exposed to the thunderstorm test twice; once at the beginning of the administration (the administration of CBD at 4.0 mg/kg/day over a 2-week-period or the administration of the same amount of sunflower oil as a placebo) and once after the end of the administration. When undesirable urination was observed, occurrences of this behaviour were found to decrease significantly when CBD was administered. However, no such changes were recorded when the placebo was administered. These results indicate that CBD could be an effective option for the treatment of noise-induced fear.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 11","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12153555/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144282169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
AnimalsPub Date : 2025-06-03DOI: 10.3390/ani15111640
Mariana Santos, Dalton Mendes de Oliveira, Matheus Rodrigues de Souza, Gabrielly Benevides de Almeida, Aylpy Renan Dutra Santos, Juliano Cesar Castro Belmonte, Fabiana de Andrade Melo-Sterza, André Luiz Julien Ferraz, Marcelo Vedovatto
{"title":"Impact of Two-Stage Weaning and Bovine-Appeasing Substance on Growth, Temperament, Pasture Behavior, and Immune System of Nellore Calves.","authors":"Mariana Santos, Dalton Mendes de Oliveira, Matheus Rodrigues de Souza, Gabrielly Benevides de Almeida, Aylpy Renan Dutra Santos, Juliano Cesar Castro Belmonte, Fabiana de Andrade Melo-Sterza, André Luiz Julien Ferraz, Marcelo Vedovatto","doi":"10.3390/ani15111640","DOIUrl":"10.3390/ani15111640","url":null,"abstract":"<p><p>This experiment evaluated the effects of a nose flap (NF) device and bovine-appeasing substance (BAS) administration on the growth, temperament, immune response, and pasture behavior of calves during weaning. A total of 24 Nellore calves were used in a 2 × 2 factorial arrangement. The factors were the use of an NF or not (NoNF) for 14 d before weaning and the administration of BASs or not (NoBAS) at weaning (d 0). NF vs. NoNF reduced (<i>p</i> ≤ 0.01) the average daily gain (ADG; d -7 to 84; -0.289 vs. 0.378 ± 0.13 kg/d) and the time ruminating but increased (<i>p</i> ≤ 0.05) the exit score from the chute (d 14 and 28) and the time grazing. BAS vs. NoBAS administration increased (<i>p</i> ≤ 0.02) the ADG (d 14 to 84; 0.487 vs. -0.08 ± 0.10 kg/d) and the time grazing, reduced (<i>p</i> < 0.01) the time ruminating, and tended to decrease (<i>p</i> ≤ 0.10) the exit score (d 7, 14 and 28) and the time vocalizing. Treatments did not affect (<i>p</i> ≥ 0.35) serum rabies titer concentration. Thus, the use of NFs reduced growth and increased stress after weaning, while BAS administration increased growth and decreased stress after weaning, altering behavior but not the immune system. According to the results of this experiment, the use of BASs but not NFs is recommended to alleviate weaning stress in beef calves.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 11","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12153807/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144282113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
AnimalsPub Date : 2025-06-03DOI: 10.3390/ani15111652
Yue Shi, Yan Liu, Yunpeng Qi, Youjun Rong, Xiaofang Ao, Mingzhu Zhang, Qincheng Xia, Yanjun Zhang, Ruijun Wang
{"title":"Estimation of Genetic Parameters of Growth Traits in the Inner Mongolia White Cashmere Goat (Erlangshan Type).","authors":"Yue Shi, Yan Liu, Yunpeng Qi, Youjun Rong, Xiaofang Ao, Mingzhu Zhang, Qincheng Xia, Yanjun Zhang, Ruijun Wang","doi":"10.3390/ani15111652","DOIUrl":"10.3390/ani15111652","url":null,"abstract":"<p><p>This study aimed to estimate the variance, heritability, and genetic correlation of growth traits in Inner Mongolia white cashmere goats (Erlangshan type) (IMWCG-ER). Data collected from the Erlang Mountain Ranch in 2022-2023 were analyzed. The traits studied included birth weight (BW), weaning weight (WW), pre-weaning daily gain (ADG), and the 12-month weight (12W). In animal models, single and multi-trait analyses were performed using the restricted maximum likelihood (REML) method to estimate genetic parameters, and six different models were fitted for each trait by ignoring or including maternal permanent environmental effects, maternal additive genetic effects, and the interaction with individual additive genetic effects. The accuracy and suitability of each model were tested using the likelihood ratio and AIC and BIC tests. The heritability estimates of birth weight, weaning weight, daily gain before weaning, and the 12-month weight were 0.3884, 0.2951, 0.2749, and 0.2192, respectively. The absolute value of the genetic correlation coefficient between traits ranged from -0.8700 to 0.5529. The highest genetic association was between birth weight and pre-weaning daily gain (-0.8700), followed by birth weight and the 12-month weight (-0.6256). The absolute value of phenotypic correlation coefficients ranged from -0.7906 to 0.3562. The highest phenotypic correlation was between birth weight and daily gain before weaning, and the correlation coefficient was -0.7906.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 11","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12153933/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144282075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
AnimalsPub Date : 2025-06-02DOI: 10.3390/ani15111634
Pantaleo Gemma, Eleonora Nannoni, Barbara Padalino, Angelo Peli, Francesco Luca Alexander, Giovanni Buonaiuto, Luca Sardi, Giovanna Martelli
{"title":"Perception and Awareness of Animal Welfare Among Residents of Malta.","authors":"Pantaleo Gemma, Eleonora Nannoni, Barbara Padalino, Angelo Peli, Francesco Luca Alexander, Giovanni Buonaiuto, Luca Sardi, Giovanna Martelli","doi":"10.3390/ani15111634","DOIUrl":"10.3390/ani15111634","url":null,"abstract":"<p><p>A representative sample of Maltese citizens (N = 384) was surveyed about their perception and attitude towards animal welfare and animal-friendly foods. Knowledge about animal welfare was self-evaluated as moderate (36%) or good (27%), and mass media (television, web and newspapers) were the primary information source (73%). Dairy cows were perceived as having the highest welfare (average rating 3 on a 1-to-5 scale), while conditions for broilers and pigs were perceived as more critical (average rating 2.7). Respondents consider animal welfare important (64%), the availability of welfare-friendly products in Malta limited (49%), and would support a national animal-friendly label (84%). Although 49% were willing to pay more for animal-friendly products, the accepted price increase was limited (increase below 10% for 37% of respondents, 20% were not willing to pay more and 27% were price-sensitive). Chi-squared analysis showed that the respondents' profession impacted the support towards an animal welfare label (<i>p</i> < 0.01), with business operators being the least interested. Urban respondents were more critical toward farm animal welfare and more supportive of establishing a national welfare label than rural respondents (<i>p</i> < 0.05). The latter were less convinced that their choices can influence the welfare of farm animals (<i>p</i> < 0.01). Women were more willing to pay for welfare improvements than men (<i>p</i> < 0.01). These findings emphasize a significant concern for animal welfare among respondents, coupled with a moderate willingness to pay. The strong support for a national animal welfare label warrants the question as to whether transparent labelling would allow consumers to translate their sensibility into ethical purchasing behaviour.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 11","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12153805/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144282165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
AnimalsPub Date : 2025-06-02DOI: 10.3390/ani15111635
Chan-Ho Kim, Woo-Do Lee, Se-Jin Lim, Ka-Young Yang, Jung-Hwan Jeon
{"title":"Effects of Cool Water Supply on Laying Performance, Egg Quality, Rectal Temperature and Stress Hormones in Heat-Stressed Laying Hens in Open-Type Laying Houses.","authors":"Chan-Ho Kim, Woo-Do Lee, Se-Jin Lim, Ka-Young Yang, Jung-Hwan Jeon","doi":"10.3390/ani15111635","DOIUrl":"10.3390/ani15111635","url":null,"abstract":"<p><p>We used an animal welfare-certified open-type layer farm and analyzed the egg production, egg quality, rectal temperature, and yolk corticosterone levels of laying hens supplied with cool water during the summer season (avg. 33 ± 3.89 °C). A total of 5750 Hy-Line Brown laying hens at 53 weeks of age were used, and two treatment groups were established: a control group (2900 hens) and a cool water treatment group (2850 hens). The water temperature of the control group was 25.3 ± 0.8 °C and the cool water was 20.1 ± 0.3 °C; all other environment parameters (lighting, ventilation, temperature, feed, etc.) were the same. The experiment was conducted for a total of 9 weeks (between July and September 2024), and during this period, the temperature-humidity index (THI) inside the breeding facility averaged 85.21, which corresponds to the cool water supply range (80 < THI < 90). As a result, the cool water treatment group maintained high productivity and showed low mortality (<i>p</i> < 0.05). In addition, hens provided with cool water showed high eggshell strength and low yolk corticosterone levels (<i>p</i> < 0.05). The core finding of this study is that the supply of cool water in summer is effective in maintaining the productivity and egg quality of laying hens and reducing HS. This is significant in that it suggests it is possible to manage laying hens in summer in a simple way, and it can also be used as basic data for designing future studies, such as using a combination of natural products including vitamins and minerals.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 11","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12153544/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144282125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}