Animals最新文献

{"title":"A Case of Acute Hyperkalaemia of Unknown Origin During General Anaesthesia in a Rottweiler.","authors":"Dany Elzahaby, Olivier Louis Levionnois, Francesca Tosi, Ute Morath-Huss","doi":"10.3390/ani15121730","DOIUrl":"10.3390/ani15121730","url":null,"abstract":"<p><p>A 4-year-old neutered female rottweiler underwent three separate anaesthetic events for the diagnosis and treatment of cranial cruciate ligament rupture. The patient was otherwise healthy, with consistently normal pre-anaesthetic electrolyte values. However, during the second anaesthetic procedure, the patient developed severe hyperkalaemia with life-threatening cardiovascular compromise. Prompt treatment led to rapid normalisation of plasma potassium levels and resolution of cardiac abnormalities. This case highlights the need for further research to elucidate the mechanisms underlying this rare but serious complication. Clinicians should remain vigilant, as intra-anaesthetic hyperkalaemia, though uncommon, can be fatal. While the condition has been historically associated with greyhounds, recently reported cases in rottweilers may warrant similar concern.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 12","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12189807/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144493478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional and Genetic Insights into the Role of the <i>NR4A1</i> Gene in the Litter Size of the Shaanbei White Cashmere Goat.","authors":"Ebadu Areb, Yutian Bi, Yangyang Bai, Qihui Zhu, Lingyuan Ma, Chuanying Pan, Xiaolei Chen, Xianyong Lan","doi":"10.3390/ani15121729","DOIUrl":"10.3390/ani15121729","url":null,"abstract":"<p><p>Nuclear receptor subfamily 4 group A member 1 (<i>NR4A1</i>) plays a crucial role in regulating various physiological processes. As gene mining for reproductive traits is essential, this study aimed to investigate the mRNA expression, genetic variation, and association of the <i>NR4A1</i> gene with goat litter size. We examined the mRNA expression levels of the <i>NR4A1</i> gene in eight different tissues of female Shaanbei White Cashmere (SBWC) goats (<i>n</i> = 6). Then, a novel 11-bp insertion/deletion (InDel) variant was genotyped in 1136 SBWC goats, 87 SNPs were identified through resequencing (<i>n</i> = 120), and selection signal analysis was undertaken. The <i>NR4A1</i> gene was expressed in all examined tissues, including the ovary and the oviduct, suggesting its role in goat reproduction. Both the 11-bp InDel and 13 SNP variants showed significant association with litter size. Additionally, four potential transcription factor binding sites were predicted within the insertion allele, which may contribute to increased litter size. Selection signal analysis revealed strong pressure on the <i>NR4A1</i> gene region in the Cashmere goat population. These findings suggest that <i>NR4A1</i> is a promising candidate gene for improving litter size in goats and could be utilized as a genetic marker in breeding programs.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 12","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12189067/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144493484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beyond the Camera Trap: A Systematic Review of Computing Technology Used to Monitor and Interact with (More) Varied Taxa in Zoos and Aquariums.","authors":"Lilliana Hassinger, Christena Nippert-Eng","doi":"10.3390/ani15121721","DOIUrl":"10.3390/ani15121721","url":null,"abstract":"<p><p>Zoos and aquariums house a wide range of species, yet research remains heavily skewed toward mammals and camera-based technologies. This systematic literature review examines the use of computing technologies to monitor or interact with animals in zoos, aquariums, or wildlife parks, with a focus on taxonomic representation and technological diversity. A total of 125 studies published between 2014 and 2024 met the inclusion criteria, encompassing 151 recorded instances of technology use. Cameras and video systems were the most frequently used tools, appearing alone in 40% of studies and in combination with other technologies in an additional 18.4%, accounting for 48.3% of all technology instances. Most studies focused on only mammals (73.5%), and behavioral monitoring was the most common research aim (40.9%). These findings suggest an uneven distribution of research shaped more by convenience and familiarity than by welfare need, highlighting a critical opportunity to diversify both species focus and technological application. Increased investment in underrepresented species and underutilized tools will help ensure that research better reflects the full spectrum of animal needs and experiences.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 12","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12189352/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144493505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-Invasive Analyses of Altered Schaedler Flora in C57Bl/6J and Balb/c Mice to Monitor Hygiene Status of a Housing Facility.","authors":"Rebecca Nistelberger, Patrizia Gibler, Lisa Barones, Arno Absenger, Julia B Kral-Pointner, Manuel Salzmann, Boris Hartmann, Bruno K Podesser, Phillip J Hohensinner, Roberto Plasenzotti","doi":"10.3390/ani15121725","DOIUrl":"10.3390/ani15121725","url":null,"abstract":"<p><p>The composition of the gut microbiome, defined by environmental factors, significantly affects research outcomes, with variations observed across animal facilities. Efforts to standardize led to the definition of the 'Altered Schaedler flora' (ASF), comprising eight bacterial groups. Our data highlights the variability of ASF under pathogen contact. Feces from two wild-type strains (<i>C57Bl/6J</i> and <i>Balb/c</i> mice) with and without proven infection was collected in two different animal facilities and analyzed. The data show a significant difference in the quantity (either reduction or increase) of the eight ASF bacterial groups when comparing infected and non-infected mice across different housing areas (SPF-specific pathogen-free, quarantine, and conventional-experimental areas) within a facility, as well as in comparison to another facility. Furthermore, strain-specific differences are also evident, with certain ASF groups showing a reduction in quantity at one facility but an increase at the other, comparing the same housing area. Comparative studies across facilities confirmed the necessity of baseline determination for accurate ASF analysis. Performing ASF analysis, facilitated by in-house qPCR (quantitative polymerase chain reaction) kits, offers prompt and precise microbiome profiling, enhancing experimental accuracy and health monitoring in animal research settings.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 12","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12189589/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144493594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Technological Solutions to Decrease Protein Content in Piglet Weaning Feed, Improving Performance, Gut Efficiency, and Environmental Sustainability.","authors":"Michele Lanzoni, Paul De Smet, Giovanni Ferrari, Davide Bochicchio, Sujen Santini, Valerio Faeti","doi":"10.3390/ani15121720","DOIUrl":"10.3390/ani15121720","url":null,"abstract":"<p><p>The trial explored innovative low-protein diets for weaning piglets, testing the elimination of fish meal and blood derivatives. The trial compared three treatments, each with two post-weaning feeds: control feed with blood plasma and fishmeal; T1: low-protein feed with seven amino acids, fishmeal, and blood plasma; T2: low-protein feed with seven amino acids without fishmeal and blood plasma. The trial tested 180 weaned piglets, 24 days of age, evenly distributed by weight, age, and litter of origin. At the end of the first phase, T1 and T2 recorded a significantly higher average weight, ADG, and FCR compared with the control feed. At the end of the second phase, the T2 group maintained a significantly better weight, ADG, and FCR than the control group. Considering the entire test period (phase 1 and phase 2), the T2 thesis appears to have a significantly higher average weight, ADG, with a lower FCR compared to the control group. The T2 thesis fixed a significantly (<i>p</i> < 0.01) greater quantity of nitrogen compared to the T1 thesis and control feed (T2 58.71% vs. T1 54.13% and control 54.02%). In conclusion, a low-protein diet without raw materials of animal origin proved more efficient in terms of performance and nitrogen retention.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 12","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12190129/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144493617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome Selection and Genome-Wide Association Analyses for Litter Size Traits in Large White Pigs.","authors":"Yifeng Hong, Xiaoyan He, Dan Wu, Jian Ye, Yuxing Zhang, Zhenfang Wu, Cheng Tan","doi":"10.3390/ani15121724","DOIUrl":"10.3390/ani15121724","url":null,"abstract":"<p><p>(1) Background: Litter size traits are critical for pig breeding efficiency but pose challenges due to low heritability and sex-limited influences. This study aimed to elucidate the genetic architecture and identify candidate genes for these traits in Large White pigs using genomic selection (GS) and genome-wide association analyses (GWAS). (2) Methods: This study utilized phenotypic data from nine litter size traits in Large White sows. Genotyping-by-sequencing (GBS) was performed to obtain genotype data, retaining 153,782 high-quality SNPs after quality control. Genetic evaluation was conducted using single-step genomic best linear unbiased prediction (ssGBLUP), with genetic parameters (heritability and genetic correlations) estimated via an animal model (repeatability model). To assess prediction accuracy, 10-fold cross-validation was employed to compare traditional BLUP with ssGBLUP. Furthermore, a single-step genome-wide association study (ssGWAS) integrated genomic information and pedigree-based relationship matrices to screen for significant SNPs associated with litter size traits across the genome. Functional analysis of key candidate genes was subsequently conducted based on ssGWAS results. (3) Results: Heritabilities for litter traits ranged from 0.01 to 0.06. ssGBLUP improved genomic prediction accuracy by 6.38-13.33% over BLUP. Six genomic windows explaining 1.07-1.77% of genetic variance were identified via ssGWAS, highlighting <i>GPR12</i> on SSC11 as a key candidate gene linked to oocyte development. (4) Conclusions: This study demonstrates the efficacy of ssGBLUP for low-heritability traits and identifies GPR12 as a pivotal gene for litter size. Prioritizing NHB and LBWT in breeding programs could enhance genetic gains while mitigating adverse effects on piglet health. These findings advance genomic strategies for improving reproductive efficiency in swine.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 12","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12189426/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144493486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-Wide Association Analysis of Flavor Precursor Traits in Chengkou Mountain Chicken.","authors":"Haiwei Wang, Yu Huang, Lingbin Liu, Xin Zhang, Donghang Deng, Zhen Wang, Guangliang Gao, Qigui Wang","doi":"10.3390/ani15121726","DOIUrl":"10.3390/ani15121726","url":null,"abstract":"<p><p>Chengkou mountain chicken, a Chinese indigenous breed, exhibits unique flavor characteristics. However, the genetic basis of its flavor precursor substances remains unexplored. We performed a genome-wide association study (GWAS) using low-coverage whole-genome sequencing (lcWGS) and genotype imputation to explore genetic markers linked to flavor precursors (nucleotides, amino acids, etc.) in Chengkou mountain chicken breast muscle. We identified 44 SNPs potentially or significantly associated with flavor precursor traits and localized 18 genes. Functional analysis revealed eight important candidate genes, including <i>ZBTB20</i>, <i>RFX4</i>, <i>MAMLD1</i>, <i>SYN3</i>, <i>ABTB3</i>, <i>PRPF39</i>, <i>LRFN5</i>, and <i>DGCR14</i>, which may play key roles in influencing flavor precursor substances. Moreover, two SNPs residing in the haplotype block (53,448,483 bp to 53,450,834 bp) on chromosome 1 were significantly associated with a di-unsaturated acyl chain (C20:2) residue and mapped to the <i>ABTB3</i> gene. This study analyzes the composition of flavor precursor substances in the pectoralis major muscle of Chengkou mountain chicken. Moreover, the SNPs, haplotypes, and candidate genes identified in this study can be used to improve the accuracy of the marker-assisted selection of traits related to flavor precursor substances in the breast muscle of chicken. In addition, the candidate genes that are significantly associated with these traits will potentially lay the foundation for future genetic selection aimed at improving the flavor traits of chicken meat.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 12","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12189354/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144493487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Blue Crab (<i>Callinectes sapidus</i>) Haemolymph as a Potential Reservoir of Mesophilic <i>Shewanella</i> Species.","authors":"Giuseppe Esposito, Fabio Bondavalli, Matteo Riccardo Di Nicola, Paolo Pastorino, Sonia Scala, Martina Gini, Giulia Milanese, Edoardo Turolla, Alessandra Maganza, Simona Sciuto, Domenico Meloni, Rita Melillo, Pierluigi Acutis, Elena Bozzetta, Sebastiano Virgilio, Caterina Faggio, Silvia Colussi, Marino Prearo","doi":"10.3390/ani15121731","DOIUrl":"10.3390/ani15121731","url":null,"abstract":"<p><p>The blue crab (<i>Callinectes sapidus</i>) is an invasive alien species in the Mediterranean Sea, posing threats to biodiversity, fisheries, and aquaculture. Climate change has worsened these challenges, influencing the distribution of bacterial species, including <i>Shewanella</i> species, which are sensitive to changes in temperature and salinity. In this study, 300 blue crabs were sampled between June and October 2024 from the Sacca di Goro (Northern Adriatic Sea, Italy) to investigate the prevalence of <i>Shewanella</i> species in their haemolymph. The prevalence was found to be 7% (21/300), with species such as <i>S. mesophila</i>, <i>S. algae</i>, <i>S. cowelliana</i>, and <i>S. baltica</i> identified, particularly in the months of September and October. Molecular techniques, including MALDI-TOF MS and <i>rpoB</i> gene amplification, were used to identify isolates. Antibiotic susceptibility testing (AST) revealed a trend of resistance to beta-lactam antibiotics. A network analysis was also conducted to examine the global trends of <i>Shewanella</i> research in relation to humans, animals, and the marine environment. While proper cooking eliminates the risk to consumers, handling without personal protective equipment can increase exposure, particularly for vulnerable individuals such as those who are elderly or immunocompromised. Mild symptoms are observed in children. Further studies, particularly with a One Health approach, are crucial to better understand the transmission dynamics and evolving antibiotic resistance of <i>Shewanella</i> species.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 12","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12189129/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144493507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histopathological Assessment and In Ovo Vaccination Response to IBD and ND in Broiler Chickens.","authors":"Marcin Wegner, Adrian Żurek, Joanna Frischke-Krajewska, Michał Gesek","doi":"10.3390/ani15121722","DOIUrl":"10.3390/ani15121722","url":null,"abstract":"<p><p>Infectious bursal disease (IBD) is a viral disease that most commonly affects young chickens and destroys lymphocytes, leading to immunosuppression. The field study aimed to investigate the effect of three different vaccines administered in ovo against IBD and spray against Newcastle disease (ND) on serological response tested for IBD and ND and histopathological analysis of the bursa of Fabricius (BF) and quantitative B lymphocytes in BF in broiler chickens. The study was conducted on a farm of four hen houses with 30,000 chicks in each building. Three different vaccination programs were used in the poultry hatchery, and one hen house IV was not vaccinated. All three groups were vaccinated at 18 days and 9 h in ovo during egg transfer against IBD at a dose of 0.05 mL/embryo, group I vector vaccine (strain vHVT013-69), group II immunocomplex vaccine (strain Winterfield 2512), group III immunocomplex vaccine (strain M.B, 0.05). Then, after hatching, the chicks were vaccinated in a spray (groups I, II, and III) against NDV (strain VG/GA, 20 mL/100 birds) and infectious bronchitis (IBV) in a spray (strain H-120, serotype Mass, and strain CR88121, serotype 793B) at a dose of 20 mL/100 chicks. On days 1, 21, 31, and 41, blood was collected for serological tests to determine the antibody titer against IBD, which was performed using two tests (IDEXX and ID-Vet) and against ND. During the necropsy of birds on days 21 and 31, the bursae of Fabricius were collected from five chickens for histopathological evaluation of BF and quantitative B lymphocyte counts; a total of 40 bursae were analyzed (10 per group). The vaccination program applied significantly (<i>p</i> < 0.05) affected the immune response expressed as a geometric mean titer (GMT) in the serum of the examined chickens against IBDV on days 21, 31, and 41. Differences were also demonstrated in the mass and level of BF damage and the number of B lymphocytes. No significant differences were demonstrated in the GMT in the serum of the examined chickens against NDV depending on the vaccination program applied.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 12","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12189615/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144493521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking Immune Signatures: Surrogate Markers for Assessing VHSV Vaccine Efficacy in Olive Flounder (<i>Paralichthys olivaceus</i>).","authors":"Ji-Min Jeong, Mun-Gyeong Kwon, Chan-Il Park","doi":"10.3390/ani15121728","DOIUrl":"10.3390/ani15121728","url":null,"abstract":"<p><p><i>Viral hemorrhagic septicemia virus</i> (VHSV) is a major pathogen in <i>Paralichthys olivaceus</i> (olive flounder) aquaculture, necessitating effective vaccines and evaluation methods. This study assessed immune responses following intraperitoneal administration of an inactivated VHSV vaccine and identified surrogate markers of vaccine efficacy. Fish were sampled weekly over 8 weeks. Antigen-specific antibody titers were measured by ELISA, and the expression of seven immune-related genes (<i>CD4</i>, <i>CD8</i>, <i>CD28</i>, <i>IgM</i>, <i>Mx</i>, <i>IFN</i>, and <i>IL-1β</i>) was analyzed in the spleen, kidney, liver, and gill by qPCR. The vaccinated group showed 84% relative survival, while unvaccinated fish experienced 100% mortality. Antibody titers peaked at 4 weeks post-vaccination, aligning with elevated <i>IgM</i> expression in the spleen and kidney. <i>CD4</i> (kidney) and <i>CD28</i> (spleen) expression strongly correlated with antibody titers (r = 0.854 and 0.796, respectively), whereas <i>IL-1β</i>, <i>IFN</i>, and <i>Mx</i> showed moderate associations in specific tissues. These findings indicate that <i>CD4, CD28</i>, and <i>IgM</i> may serve as molecular indicators of humoral immune responses specifically following inactivated VHSV vaccination in olive flounder (<i>Paralichthys olivaceus</i>). Combining gene expression profiling with antibody analysis offers a non-lethal, practical approach to evaluating vaccine efficacy in aquaculture.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 12","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12189318/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144493645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}