Animals最新文献

{"title":"One Health, Two Species: Linking Domestication to Cognitive Aging in Dogs and Humans.","authors":"Corinne Quadalti","doi":"10.3390/ani15192851","DOIUrl":"10.3390/ani15192851","url":null,"abstract":"<p><p>This commentary explores the parallel neuroanatomical and neurobiological evolution that ultimately led to modern dogs and humans, through domestication and self-domestication, respectively. The selective pressures for benignness and enhanced prosociality have reshaped brain anatomy and cognitive architecture in both <i>Canis familiaris</i> and <i>Homo sapiens</i>, leading to analogous changes including a reduction in limbic system volume and expansion of the prefrontal cortex, critical for executive control and social cognition. From a molecular point of view, shared genetic and epigenetic underpinnings of these adaptations and their implications gave rise to parallel trajectories in brain aging; notably, the emergence of canine cognitive dysfunction. Interestingly, this canine age-related cognitive decline presents significant overlaps with Alzheimer's disease in terms of both behavioral presentation and underlying pathology. In the context of a One Health perspective, the profound influence of shared environmental exposures, such as urbanization, pollutants, and stressors, on neurodevelopment, cognitive aging, and disease susceptibility offers a compelling translational model for understanding brain health within intertwined ecological and social contexts.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 19","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12523904/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145298398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wnt/β-Catenin Pathway Activation Confers Fumonisin B1 Tolerance in Chicken Intestinal Organoid Monolayers by Enhancing Intestinal Stem Cell Function.","authors":"Shuai Zhang, Yanan Cao, Yiyi Shan, Xueli Zhang, Liangxing Xia, Haifei Wang, Shenglong Wu, Wenbin Bao","doi":"10.3390/ani15192850","DOIUrl":"10.3390/ani15192850","url":null,"abstract":"<p><p>Fumonisin B1 (FB1) is a prevalent mycotoxin in moldy grains and feeds, highly toxic to livestock and compromising product quality while threatening food safety. Poultry exhibit low susceptibility to FB1, but the underlying tolerance mechanisms remain unclear. Traditional 3D chicken intestinal organoid models cannot simulate direct interaction between the epithelial monolayer and FB1, limiting the study of FB1-chicken intestinal crosstalk. Here, we established a 2D chicken intestinal organoid monolayer model, derived from intestinal crypts of 18-day-old specific pathogen-free chicken embryos, to systematically explore poultry's resistance mechanisms against FB1. Using this model, we compared FB1-induced effects with those in a porcine intestinal epithelial cell model. Results showed that FB1 exposure did not reduce transepithelial electrical resistance, induce abnormal expression of tight junction genes, or cause significant fluctuations in inflammatory factor levels in chicken intestinal organoid monolayers. Mechanistically, FB1 enhances chicken intestinal stem cell function by activating the Wnt/β-catenin pathway, thereby promoting epithelial regeneration and renewal to increase FB1 resistance and decrease toxin sensitivity in chickens. This study reveals a strategy for enhancing FB1 tolerance in poultry by promoting intestinal stem cell function, providing a new perspective for developing mycotoxin prevention and control strategies.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 19","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12523766/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145298511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Short-Term Treatment of Hanwoo Satellite Cells with Various Concentrations of Cortisol.","authors":"Leecheon Kim, Dongjin Yu, Hyunwoo Choi, Jongryun Kim, Junseok Ban, Kwanseob Shim, Darae Kang","doi":"10.3390/ani15192847","DOIUrl":"10.3390/ani15192847","url":null,"abstract":"<p><p>Transportation, environmental changes, and overcrowding can induce short-term stress in livestock, leading to physiological imbalances even within a short period. Cortisol is a stress-response hormone and its concentration in the blood can rapidly fluctuate depending on the individual and situation. This study evaluated the short-term effects of cortisol by applying blood cortisol concentrations that mimicked the normal and stress-induced levels observed in Korean native cattle (Hanwoo) to the culture medium of Hanwoo muscle stem cells (HWSC). Treatments were designed with five cortisol concentrations (0, 5, 10, 30, and 70 ng/mL) and four treatment times (0.5, 1, 2, and 3 h), based on the CCK-8 and viable cell count results. The expression levels of cortisol receptor-related genes (<i>NR3C1</i>, <i>HSP70</i>, and <i>HSP90AA1</i>) increased and reached a peak at 30 min post-treatment. After 30 min, the expression of these genes gradually decreased. However, in the case of <i>HSP70</i>, expression tended to increase again after 3 h of treatment. This could be seen as the regulation of cortisol inflow into the HWSC. Upon examining the oxidative effects of cortisol on superoxide dismutase 1 (<i>SOD1</i>), glutathione peroxidase (<i>GPX</i>), catalase (<i>CAT</i>), and oxygen consumption rate (OCR), the expression of antioxidant factors increased and peaked at 30 min of treatment. Following this peak, their levels generally began to decrease. However, in the 70 ng/mL group, the expression of these factors remained at a high level compared to the control group even after 30 min. In addition, the cellular respiration index and ATP production increased as the treatment prolonged, regardless of the concentration, as shown by the OCR analysis. These results can be considered a phenomenon corresponding to the accumulation of oxidative by products, such as Reactive Oxygen Species (ROS), caused by cortisol. The gene expression of apoptosis factors (<i>p53</i>, <i>BAX</i>, <i>Caspase-3</i>) temporarily increased at 30 min but then decreased. Caspase-3 protein activity was elevated at 30 min in the 70 ng/mL group, which later reduced. These results suggested that short-term cortisol administration had no effect on apoptosis in muscle cell culture. Therefore, the study findings elucidating the effects of short-term cortisol treatment on HWSC suggest that short-term stress may not have a significant negative effect on Hanwoo muscle. However, as this study was limited to muscle stem cells derived from Hanwoo, further investigation is required to determine whether the observed responses are consistent across different species and in vivo environments.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 19","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12524049/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145298173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carcass Traits and Meat Quality of Surgically Castrated and Immunocastrated Pigs at Two Slaughter Weights.","authors":"Dmytro V Zhdanov, Oleksandr H Mykhalko, Mykola H Povod, Galia Zamaratskaia","doi":"10.3390/ani15192846","DOIUrl":"10.3390/ani15192846","url":null,"abstract":"<p><p>Surgical castration of male piglets is a common practice to prevent boar taint and reduce aggressive behaviour. However, it raises welfare concerns and alters carcass fat deposition. Immunocastration, a vaccine-based alternative targeting gonadotropin-releasing hormone (GnRH), mitigates these welfare issues. This study evaluated carcass traits and meat quality in surgically and immunocastrated pigs slaughtered at two weight classes (approximately 116 kg and 136 kg). We compared growth performance, carcass composition, fat quality, and key meat quality indicators among surgically castrated males, immunocastrated males, and immunocastrated females. Inclusion of uncastrated and immunocastrated females provides novel comparative data for mixed-sex production systems, where such information is scarce. This broader evaluation helps fill current gaps in knowledge about immunocastration effects in female pigs. Surgically castrated males showed higher backfat thickness and fat content, particularly at the heavier weight, while immunocastrated pigs exhibited intermediate traits. Ultimate pH, colour, marbling, water-holding capacity, and moisture loss varied with castration method, sex, and slaughter weight, though many differences were subtle. The findings confirm that immunocastration offers a favourable balance between animal welfare and production traits, producing pork quality comparable to surgical castration. These results provide valuable insights for optimizing pork production systems, balancing welfare, efficiency, and meat quality.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 19","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12523624/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145298057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Goblet Cells and Mucus Composition in Jejunum and Ileum Containing Peyer's Patches and in Colon: A Study in Pigs.","authors":"Vladimir Ginoski, José Luis Cortés Sánchez, Stefan Kahlert, Johannes Schulze Holthausen, Łukasz Grześkowiak, Jürgen Zentek, Hermann-Josef Rothkötter","doi":"10.3390/ani15192852","DOIUrl":"10.3390/ani15192852","url":null,"abstract":"<p><p>The intestinal mucus layer is a dynamic protective barrier that maintains gut homeostasis, supports immune defense, and regulates host-microbiota interactions. Rodent models have yielded valuable insights, but their intestinal structure and physiology differ from those of humans and pigs. By contrast, the omnivorous pig shares closer anatomical and immunological features with humans, making it a relevant large-animal model in translational studies. In this study, we established a histological workflow for porcine intestine by combining Carnoy's fixation with Alcian Blue-Periodic Acid-Schiff and Mucicarmine staining. This enabled accurate visualization and quantification of goblet-cell density and mucus thickness across intestinal segments, with a particular focus on Peyer's patches-key sites of immune surveillance. Both stains produced consistent results. We observed a clear proximal-to-distal gradient, from jejunum to colon, in mucus thickness: the colon displayed the thickest layer (~100 μm), whereas the follicle-associated epithelium over Peyer's patches in the jejunum and ileum showed a markedly thinner layer (<12 μm) and fewer goblet cells. Immunofluorescence further revealed strong cytokeratin-18 expression in goblet cells, delineating their morphology and polarity. These findings demonstrate region-specific differences in mucus architecture and goblet-cell distribution that likely reflect specialized immune functions, advancing our understanding of the intestinal barrier and informing future strategies to support gut health and immunity.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 19","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12524252/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145298446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SATSN: A Spatial-Adaptive Two-Stream Network for Automatic Detection of Giraffe Daily Behaviors.","authors":"Haiming Gan, Xiongwei Wu, Jianlu Chen, Jingling Wang, Yuxin Fang, Yuqing Xue, Tian Jiang, Huanzhen Chen, Peng Zhang, Guixin Dong, Yueju Xue","doi":"10.3390/ani15192833","DOIUrl":"10.3390/ani15192833","url":null,"abstract":"<p><p>The daily behavioral patterns of giraffes reflect their health status and well-being. Behaviors such as licking, walking, standing, and eating are not only essential components of giraffes' routine activities but also serve as potential indicators of their mental and physiological conditions. This is particularly relevant in captive environments such as zoos, where certain repetitive behaviors may signal underlying well-being concerns. Therefore, developing an efficient and accurate automated behavior detection system is of great importance for scientific management and welfare improvement. This study proposes a multi-behavior automatic detection method for giraffes based on YOLO11-Pose and the spatial-adaptive two-stream network (SATSN). Firstly, YOLO11-Pose is employed to detect giraffes and estimate the keypoints of their mouths. Observation-Centric SORT (OC-SORT) is then used to track individual giraffes across frames, ensuring temporal identity consistency based on the keypoint positions estimated by YOLO11-Pose. In the SATSN, we propose a region-of-interest extraction strategy for licking behavior to extract local motion features and perform daily behavior classification. In this network, the original 3D ResNet backbone in the slow pathway is replaced with a video transformer encoder to enhance global spatiotemporal modeling, while a Temporal Attention (TA) module is embedded in the fast pathway to improve the representation of fast motion features. To validate the effectiveness of the proposed method, a giraffe behavior dataset consisting of 420 video clips (10 s per clip) was constructed, with 336 clips used for training and 84 for validation. Experimental results show that for the detection tasks of licking, walking, standing, and eating behaviors, the proposed method achieves a mean average precision (mAP) of 93.99%. This demonstrates the strong detection performance and generalization capability of the approach, providing robust support for automated multi-behavior detection and well-being assessment of giraffes. It also lays a technical foundation for building intelligent behavioral monitoring systems in zoos.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 19","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12524285/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145298205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unroofed Coronary Sinus in a Dog: Diagnostic Utility of ECG-Gated Computed Tomography.","authors":"Nanaha Ito, Risa Okamoto, Kazumi Shimada, Daigo Azakami, Zeki Yilmaz, Ryou Tanaka, Lina Hamabe","doi":"10.3390/ani15192834","DOIUrl":"10.3390/ani15192834","url":null,"abstract":"<p><p>A Labrador Retriever (4-year-old, castrated male) with signs of fatigue was diagnosed with an atrial septal defect at his primary veterinary clinic. Due to the uncertainty of this diagnosis, he was referred to the Tokyo University of Agriculture and Technology Animal Medical Center for further investigation. Transthoracic echocardiography performed on arrival showed an irregular blood flow from the left atrium (LA) to the right atrium (RA), yet no opening was found in the septum. An electrocardiogram (ECG)-gated computed tomography (CT) exam revealed a communication between the coronary sinus (CS) and the LA, causing a shunt between the LA and the RA. A diagnosis of unroofed coronary sinus syndrome (UCSS) was made. The dog's condition was stable and plans to keep observations were made. This is the first case of UCSS diagnosed with an ECG-gated CT exam.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 19","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12523538/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145298497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Habitat Shifts in the Pacific Saury (<i>Cololabis saira</i>) Population in the High Seas of the North Pacific Under Medium-to-Long-Term Climate Scenarios Based on Vessel Position Data and Ensemble Species Distribution Models.","authors":"Hanji Zhu, Yuyan Sun, Yang Li, Delong Xiang, Ming Gao, Famou Zhang, Jianhua Wang, Sisi Huang, Heng Zhang, Lingzhi Li","doi":"10.3390/ani15192828","DOIUrl":"10.3390/ani15192828","url":null,"abstract":"<p><p>Global climate change poses a significant management challenge for vital transboundary resources like the Pacific saury (<i>Cololabis saira</i>). To address this, we developed an innovative framework that uses high-resolution Automatic Identification System (AIS) data and deep learning to define species distribution, which then informs a robust Ensemble Species Distribution Model (ESDM). The model (TSS > 0.89, AUC > 0.97) identifies sea surface temperature (SST) and chlorophyll-a (CHL) as key habitat drivers. Projections under future climate scenarios reveal two critical threats: (1) a continuous northeastward migration of the habitat's centroid, exceeding 400 km by 2100 under a high-emission SSP5-8.5 scenario, and (2) a drastic contraction of highly suitable habitat (suitability > 0.8), shrinking by up to 94% under the high-emission SSP3-7.0 scenario. By directly linking key oceanographic features to these climate-driven risks, this study delivers an essential scientific decision-support tool for management bodies like the North Pacific Fisheries Commission (NPFC) to develop climate-adaptive strategies.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 19","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12523842/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145298119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sericin Enhances Cryopreserved Sperm Quality in Chengde Hornless Black Goats by Increasing Glutamine Metabolism.","authors":"Yang Yu, Wei Xia, Wentao Zhang, Chenyu Tao, Xiaofeng Tian, Mengqi Li, Xiaosheng Zhang, Jinlong Zhang, Shunran Zhao, Yatian Qi, Tianmiao Qin, Junjie Li","doi":"10.3390/ani15192830","DOIUrl":"10.3390/ani15192830","url":null,"abstract":"<p><p>The cryopreservation of Chengde polled goat semen plays a critical role in conserving genetic resources, enhancing the utilization efficiency of superior breeding bucks, and advancing artificial insemination techniques. However, spermatozoa are vulnerable to oxidative stress during the freezing process, which can significantly compromise sperm motility. In this study, pooled ejaculates from multiple bucks were divided into six groups, including a control group cryopreserved with conventional extender and five treatment groups supplemented with sericin at concentrations of 0.2%, 0.4%, 0.6%, 0.8%, and 1.0% (<i>w</i>/<i>v</i>). The results demonstrated that supplementation of the semen cryoprotectant with 0.6% sericin significantly improved post-thaw sperm viability to 65.25% in Chengde hornless goats, while concurrently reducing both the sperm abnormality rate (<i>p</i> < 0.05) and intracellular ROS levels (<i>p</i> < 0.05). Integrated TMT proteomics and LC/MS metabolomics further compared the 0.6% sericin group with the frozen control group and identified 162 differentially expressed proteins and 109 differential metabolites between the sericin supplementation and frozen control groups. Functional analysis revealed the significant enrichment of differential metabolites, such as glutamine, in the alanine, aspartate, and glutamate metabolism pathway, concomitant with the marked upregulation of antioxidant proteins including LRP8, GSTM3, and SIRT2. Thus, 0.6% sericin enhances cryotolerance primarily by improving sperm viability, reducing oxidative damage, and sustaining energy metabolism. These findings indicate that sericin enhances cryotolerance by reducing oxidative damage and supporting metabolic function, providing preliminary molecular insights for improving goat semen cryopreservation.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 19","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12523751/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145298380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Farm-Specific Effects in Predicting Mastitis by Applying Machine Learning Models to Automated Milking System and Other Farm Management Data.","authors":"Muhammad N Dharejo, Olivier Kashongwe, Thomas Amon, Tina Kabelitz, Marcus G Doherr","doi":"10.3390/ani15192825","DOIUrl":"10.3390/ani15192825","url":null,"abstract":"<p><p>Early and accurate prediction of mastitis is crucial in effective herd management and minimizing economic losses. This study investigated the effects of farm-specific factors on the accuracy of mastitis predictions by applying machine learning (ML) models to an automated milking system (AMS) and farm management data. We analyzed a large dataset consisting of 5.88 million observations over the period of 2019-2024 from four dairy farms in Germany. Six ML algorithms were applied to predict mastitis occurrence, with a focus on understanding how farm-specific factors like herd size, management practices, and farm environment may influence prediction accuracy. For training and testing on combined data, the accuracy, sensitivity and specificity ranged between 83 and 92%, 78 and 93% and 83 and 92%, respectively, with an area under curve (AUC) between 91 and 96%. However, under mixed-to-individual farm effects analysis, results exposed weaknesses in the generalization. Models adapted well to internal patterns when analyzing each individual farm separately, reaching very high AUCs of up to 98%, but the results were significantly different again when analyzed with a leave-one-out approach. The analysis determined that data from each farm carries variable underlying patterns, suggesting that a tailored approach to each farm's unique characteristics might improve mastitis prediction through ML.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 19","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12523250/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145298211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}