IF 2.7 2区农林科学

Animals Pub Date : 2025-05-01 DOI: 10.3390/ani15091312

Ebuderda Günay, Tomasz Szara, Buket Çakar, Emine İrem Deveci, Ali Serhan Coşkun, Gökhan Gün, Funda Yiğit, Ozan Gündemir, Sokol Duro, Mihaela Claudia Spataru

{"title":"Geometric Morphometric Analysis of Sexual Dimorphism in the Bill of the White Stork (Ciconia ciconia).","authors":"Ebuderda Günay, Tomasz Szara, Buket Çakar, Emine İrem Deveci, Ali Serhan Coşkun, Gökhan Gün, Funda Yiğit, Ozan Gündemir, Sokol Duro, Mihaela Claudia Spataru","doi":"10.3390/ani15091312","DOIUrl":"10.3390/ani15091312","url":null,"abstract":"Sexual dimorphism in bill morphology influences foraging strategies and bird competitive interactions. This study uses geometric morphometric analyses to examine sex-based differences in the bill shape and size of Ciconia ciconia, a large wading bird. Standardized dorsal and lateral photographs of 45 white storks (24 females and 21 males) were analyzed. The mean centroid size (CS) confirmed that males have significantly larger bills than females, yet principal component analysis revealed no reliable shape-based sex differentiation. Allometric analyses indicated that larger individuals exhibit more pronounced shape variations, likely tied to functional adaptations, though these were insufficient for sex determination. While bill size correlates with sex, shape variation does not serve as a reliable criterion for sexing C. ciconia. This study contributes to the understanding of sexual dimorphism in Ciconia ciconia by quantifying bill size differences using geometric morphometrics.","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 9","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12070864/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143967187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated Transcriptomic Analysis of Liver and Muscle Tissues Reveals Candidate Genes and Pathways Regulating Intramuscular Fat Deposition in Beef Cattle. 肉牛肝脏和肌肉组织的综合转录组学分析揭示了调节肌肉内脂肪沉积的候选基因和途径。

IF 2.7 2区农林科学

Animals Pub Date : 2025-04-30 DOI: 10.3390/ani15091306

Siwei Wang, Tingting Liu, Peng Peng, Yurong Fu, Shaoqing Shi, Shuang Liang, Xi Chen, Kun Wang, Rongyan Zhou

{"title":"Integrated Transcriptomic Analysis of Liver and Muscle Tissues Reveals Candidate Genes and Pathways Regulating Intramuscular Fat Deposition in Beef Cattle.","authors":"Siwei Wang, Tingting Liu, Peng Peng, Yurong Fu, Shaoqing Shi, Shuang Liang, Xi Chen, Kun Wang, Rongyan Zhou","doi":"10.3390/ani15091306","DOIUrl":"10.3390/ani15091306","url":null,"abstract":"Intramuscular fat (IMF) content in beef cattle is a critical determinant of beef meat quality, as it positively influences juiciness, tenderness, and palatability. In China, the crossbreeding of Wagyu and Angus is a prevalent method for achieving a better marbling level. However, the molecular mechanisms governing IMF regulation in these crossbreeds remain poorly understood. To elucidate the mechanism of IMF deposition in these crossbred cattle, we conducted a comparative transcriptomic analysis of longissimus dorsi muscles and livers from cattle with divergent IMF content. RNA-seq revealed 940 and 429 differentially expressed genes (DEGs) in the liver and muscle, respectively, with 60 genes co-differentially expressed (co-DEGs) in both tissues. Functional enrichment highlighted lipid metabolism pathways including fatty acid β-oxidation, PPAR signaling, and glycerolipid metabolism. A total of eleven genes including ACAA2, ACADL, ACOX2, CPT1B, CPT2, LPL, SLC27A1, ACAT1, GK, ACOX3, and ACSM5, were screened as key candidate genes for IMF deposition. A \"liver-muscle\" regulatory network of IMF deposition was built to illustrate the tissues' interaction. The reliability of the transcriptomic data was verified by quantitative reverse real-time PCR (qRT-PCR). Our findings provide novel molecular markers for increasing the IMF content and accelerating the genetic improvement of beef quality traits in crossbred cattle.","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 9","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12071110/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143962670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated Multi-Tissue Lipidomics and Transcriptomics Reveal Differences in Lipid Composition Between Mashen and Duroc × (Landrace × Yorkshire) Pigs. 综合多组织脂质组学和转录组学揭示马胜猪和杜洛克×（长×大）猪脂质组成差异。

IF 2.7 2区农林科学

Animals Pub Date : 2025-04-30 DOI: 10.3390/ani15091280

Mingyue Shi, Wenxia Li, Shuai Yang, Qipin Lv, Jingxian Yang, Di Sun, Guanqing Yang, Yan Zhao, Wanfeng Zhang, Meng Li, Yang Yang, Chunbo Cai, Pengfei Gao, Xiaohong Guo, Bugao Li, Guoqing Cao

{"title":"Integrated Multi-Tissue Lipidomics and Transcriptomics Reveal Differences in Lipid Composition Between Mashen and Duroc × (Landrace × Yorkshire) Pigs.","authors":"Mingyue Shi, Wenxia Li, Shuai Yang, Qipin Lv, Jingxian Yang, Di Sun, Guanqing Yang, Yan Zhao, Wanfeng Zhang, Meng Li, Yang Yang, Chunbo Cai, Pengfei Gao, Xiaohong Guo, Bugao Li, Guoqing Cao","doi":"10.3390/ani15091280","DOIUrl":"10.3390/ani15091280","url":null,"abstract":"Chinese native pig breeds exhibit unique advantages over Western pig breeds, but the specific lipid metabolism mechanisms remain unclear. The phenotypic characteristics of Mashen (MS) pigs and Duroc × (Landrace × Yorkshire) (DLY) pigs are studied. The results show that MS pigs exhibit higher intramuscular fat (IMF) content. The area of adipocytes of MS pigs is significantly greater than that in DLY pigs (p < 0.01). Lipidomics analysis reveals distinct profiles in the upper layer of backfat (ULB), leaf lard (LL), greater omentum (GOM), and IMF, with MS pigs showing higher polyunsaturated fatty acids (PUFAs) in ULB, LL, and GOM. Key differential lipids identified in the two pig breeds include the following triglycerides (TGs) and phosphatidylcholines (PC): TG(16:1_18:1_18:3), TG(18:1_18:2_18:3), TG(18:3_18:2_18:2), PC(18:0_18:1), and PC(18:0_18:2). Weighted gene co-expression network analysis (WGCNA) reveals lipid molecules associated with serum biochemical indices. Transcriptomics analysis highlights 1944 differentially expressed genes between the MS-ULB and DLY-ULB. Notably, multiple genes from the cytochrome P450 family (CYP2E1, CYP4A24, CYP2J2), along with PLA2G2D, PLA2G4A, and multiple PCs, are associated with the metabolism of arachidonic acids and linoleic acids. PLA2G2D and PLA2G4A are also involved in the metabolism of α-linolenic acids. This comprehensive analysis provides essential information for breeding strategies and meat quality improvement.","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 9","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12071155/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143960092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Early Methionine Supplementation of Ewe Lambs (F0) Modifies Meat Quality Traits of the Progeny (F1, Male Fattening Lambs). 母羊羔羊（F0）早期补充蛋氨酸对后代（F1，公育羔）肉质性状的影响。

IF 2.7 2区农林科学

Animals Pub Date : 2025-04-30 DOI: 10.3390/ani15091290

Mahsa Dehnavi, Javier Mateo, Alba Martín, F Javier Giráldez, Irma Caro, Lara Morán, Sonia Andrés

{"title":"The Early Methionine Supplementation of Ewe Lambs (F0) Modifies Meat Quality Traits of the Progeny (F1, Male Fattening Lambs).","authors":"Mahsa Dehnavi, Javier Mateo, Alba Martín, F Javier Giráldez, Irma Caro, Lara Morán, Sonia Andrés","doi":"10.3390/ani15091290","DOIUrl":"10.3390/ani15091290","url":null,"abstract":"Adequate management of replacement ewe lambs (F0) in dairy sheep farms during postnatal life may modify the germline cells, thus promoting transmission of intergenerational effects to the offspring (F1). To test this hypothesis, 18 newborn male lambs (F1), either born from methionine-supplemented ewe lambs (F0 ewe lambs being fed ad libitum with a milk replacer supplemented with 1 g methionine/kg DM) or not supplemented (F0 ewe lambs being fed ad libitum with the same milk replacer with no methionine added), were included in the present study. All the male F1 lambs were managed exactly in the same way along the whole lifespan in order to bring out the differences caused by methionine supplementation of F0 dams. Our data show that the methionine supplementation of dams (F0) during the suckling period did not promote significant (p > 0.05) changes on feed intake, growth rate, or feed efficiency of F1 male lambs during the fattening period. Moreover, the meat chemical composition (proximal, fatty acid profile, and volatile compounds) was similar for both groups (p > 0.05), but the meat of F1-MET lambs presented higher redness and hardness (p < 0.05) when compared to F1-CTRL lambs. The biochemical profile also highlighted significant (p < 0.05) differences in the serum creatinine and calcium content that may be at least partially related to the meat quality traits observed. Overall, all these results suggest that methionine supplementation of lambs (F0) during early postnatal life causes permanent changes in the offspring. This has positive effects, such as achieving a more attractive color of lamb meat (F1) for consumers, and negative effects, such as reduced meat tenderness.","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 9","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12071116/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143967222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effect of the Use of a Settling Chamber in the Cultivation of Penaeus vannamei and Salicornia neei in Aquaponics with Bioflocs. 沉降箱在生物絮凝水培中栽培凡纳滨对虾和海角鱼的效果。

IF 2.7 2区农林科学

Animals Pub Date : 2025-04-30 DOI: 10.3390/ani15091294

Isabela Pinheiro, Flávia Banderó Höffling, Felipe Boéchat Vieira, Walter Quadros Seiffert

{"title":"The Effect of the Use of a Settling Chamber in the Cultivation of Penaeus vannamei and Salicornia neei in Aquaponics with Bioflocs.","authors":"Isabela Pinheiro, Flávia Banderó Höffling, Felipe Boéchat Vieira, Walter Quadros Seiffert","doi":"10.3390/ani15091294","DOIUrl":"10.3390/ani15091294","url":null,"abstract":"This study aimed to evaluate the effect of the continuous use of the settling chamber for solids removal in the cultivation of the marine shrimp Penaeus vannamei and the halophyte Salicornia neei in an aquaponic system with bioflocs. Two treatments were tested: with settling and without settling. Each experimental unit consisted of an 800 L tank for shrimp rearing (stocking density of 375 shrimp m-3) and a hydroponic bench of 0.33 m2 for 28 seedlings (84 plants m-2). In the treatment without settling, water was continuously pumped to the hydroponic bench. In the treatment with settling, the water was first pumped to the chamber, and the overflow was then distributed across each irrigation channel, returning to the tank by gravity. To maintain the concentration of suspended solids in the shrimp culture, solids that accumulated in the settling chamber were pumped back into the tank every 30 min. During the 54-day trial, the reduction in suspended solids in the treatment with settling led to an increase in TAN and NO2 levels, while the concentration of NO3 remained stable. Although water quality parameters were more stable in the treatment without settling, no significant differences were observed between the treatments regarding plant and shrimp production indices. These results demonstrate the feasibility of cultivating P. vannamei and S. neei in a biofloc-based aquaponic system without the continuous use of a settling chamber during the pre-grow phase (until 10 g), offering a potential method for simplifying aquaponic system design.","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 9","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12070975/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143967355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hematological and Biochemical Parameters of Subadult Captive Siberian Tigers (Panthera tigris altaica). 圈养东北虎亚成年的血液学和生化指标。

IF 2.7 2区农林科学

Animals Pub Date : 2025-04-30 DOI: 10.3390/ani15091299

Xin Liu, Izhar Hyder Qazi, Haijun Wang, Zhiqiang Han, Xiao Li, Xiuli Zhang, Rui Du, Naiquan Yao, Chao Xu

{"title":"Hematological and Biochemical Parameters of Subadult Captive Siberian Tigers (Panthera tigris altaica).","authors":"Xin Liu, Izhar Hyder Qazi, Haijun Wang, Zhiqiang Han, Xiao Li, Xiuli Zhang, Rui Du, Naiquan Yao, Chao Xu","doi":"10.3390/ani15091299","DOIUrl":"10.3390/ani15091299","url":null,"abstract":"The conservation of Siberian tigers, the apex predators in the Asia-Pacific region, hinges on a thorough understanding of their physiological statuses and adaptive mechanisms. In order to fill a critical gap in our knowledge of this endangered species, we conducted comprehensive analyses of Siberian tiger whole blood and serum using modern hematology and biochemical analyzers. The blood samples were collected from subadult (2-3 years old) Siberian tigers (n = 15 for hematological analysis and n = 7 for biochemical analysis). In the present study, distinct hematological patterns including an elevated mean platelet volume (MPV) and a higher red cell distribution width coefficient of variation (RDW-CV) were observed, which were possibly triggered by pre-anesthesia resistance movements. Notably, the neutrophil percentage in Siberian tigers was found to be significantly higher than that in domestic cats and Bengal tigers, suggesting species-specific immune adaptations. In addition, serum biochemical analysis provided refined parameter ranges for subadult tigers. Unlike previous studies encompassing a broader age range, our data specifically characterize biochemical profiles in subadult tigers, offering vital baselines for health monitoring and conservation strategies. These results not only enhance our understanding of the biology of Siberian tigers but also provide scientific support for targeted conservation efforts, fostering population recovery and ecological balance in their natural habitats.","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 9","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12071179/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143967188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic Diversity and Population Structure of Acanthopagrus latus in the South China Sea. 南海棘鱼的遗传多样性和种群结构。

IF 2.7 2区农林科学

Animals Pub Date : 2025-04-30 DOI: 10.3390/ani15091295

Cheng-He Sun, Qun Zhang, Chang-Hu Lu

{"title":"Genetic Diversity and Population Structure of Acanthopagrus latus in the South China Sea.","authors":"Cheng-He Sun, Qun Zhang, Chang-Hu Lu","doi":"10.3390/ani15091295","DOIUrl":"10.3390/ani15091295","url":null,"abstract":"The yellowfin seabream Acanthopagrus latus (Houttuyn, 1782) belongs to the order Spariformes and family Sparidae and is tender, rich in fat, and nutritious, making it a high-value seafood variety along the China coast. The fry can be used for large-scale aquaculture in both seawater and freshwater. Due to environmental pollution and excessive fishing, natural A. latus populations have declined significantly in recent years, which severely depletes genetic diversity. Here, we analyzed the genetic structure of four wild A. latus populations in the South China Sea to provide insights into the genetic resources. In particular, we analyzed 40 samples from four sampling sites through whole-genome resequencing, yielding 515 Gb of raw data and 132,505,081 single nucleotide polymorphisms. Population structure, phylogenetic, and principal component analyses revealed that the four populations were homogeneous and did not show clustering based on geographical origin, indicating extensive admixture. This could be attributed to recent enhancement and release activities in the South China Sea. This information may help to protect and utilize A. latus germplasm resources.","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 9","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12071160/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143960020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From Livestock to Companion: Admission Causes, Diagnostics, and Clinical Findings in Chickens Admitted to the Avian Clinic of the Vetmeduni Vienna, 2009-2019. 从牲畜到伴侣：2009-2019年维也纳Vetmeduni禽类诊所收治鸡的入院原因、诊断和临床结果。

IF 2.7 2区农林科学

Animals Pub Date : 2025-04-30 DOI: 10.3390/ani15091288

Cornelia Konicek, Anja Joachim, Joachim Spergser, Barbara Richter, Michaela Gumpenberger

{"title":"From Livestock to Companion: Admission Causes, Diagnostics, and Clinical Findings in Chickens Admitted to the Avian Clinic of the Vetmeduni Vienna, 2009-2019.","authors":"Cornelia Konicek, Anja Joachim, Joachim Spergser, Barbara Richter, Michaela Gumpenberger","doi":"10.3390/ani15091288","DOIUrl":"10.3390/ani15091288","url":null,"abstract":"This retrospective study investigated the clinical presentation, diagnostic findings, and treatment outcomes in 419 backyard (pet) chickens admitted to the Service for Avian and Reptiles at the University of Veterinary Medicine Vienna from 1 May 2009 to 30 April 2019. The median age of the chickens was 1.5 years, with 83.8% being female. The majority (80.0%) presented with individual clinical issues, while 20.0% were diagnosed with flock diseases. The most common admission causes included unspecific clinical signs (57.3%), respiratory tract issues (18.4%), and locomotor problems (16.2%). Diagnostic imaging modalities such as ultrasonography, radiography, and CT scans revealed pathologies, including ascites, salpingitis, and skeletal system abnormalities. Parasitological examinations found a high rate of coccidia, Capillaria, and Ascaridia, while microbiological analysis identified Escherichia coli and Pasteurella multocida as common bacterial pathogens. The most prevalent diseases were upper respiratory tract infections (13.8%), egg peritonitis (9.9%), and soft tissue trauma (9.4%). Treatment outcomes showed that 67.5% survived to discharge, while 32.2% died or were euthanized. Surgical interventions were performed in 25.3% of cases, with the most common surgeries being salpingohysterectomy and wound debridement. This study highlights the wide range of health issues faced by pet chickens and emphasizes the importance of accurate diagnosis and targeted treatment in avian veterinary care. It also underscores the role of various diagnostic tools, such as imaging, pathogen detection, and histopathology, in addressing the health challenges of backyard chickens.","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 9","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12071008/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Food Restriction Induces Changes in Ovarian Folliculogenesis, Cell Proliferation, Apoptosis, and Production of Regulatory Peptides in Rabbits. 食物限制诱导兔卵巢卵泡发生、细胞增殖、凋亡和调节肽产生的变化。

IF 2.7 2区农林科学

Animals Pub Date : 2025-04-30 DOI: 10.3390/ani15091282

Imane Hadjadj, Zuzana Fabova, María-Luz García, Iván Agea, Barbora Loncová, Martin Morovic, Peter Makovicky, María-José Argente, Alexander V Sirotkin

{"title":"Food Restriction Induces Changes in Ovarian Folliculogenesis, Cell Proliferation, Apoptosis, and Production of Regulatory Peptides in Rabbits.","authors":"Imane Hadjadj, Zuzana Fabova, María-Luz García, Iván Agea, Barbora Loncová, Martin Morovic, Peter Makovicky, María-José Argente, Alexander V Sirotkin","doi":"10.3390/ani15091282","DOIUrl":"10.3390/ani15091282","url":null,"abstract":"The aim of this study is to examine the influence of food restriction on rabbit ovarian functions. A total of eight females were fed ad libitum (NF), while eight females were subjected to 50% food restriction (RF). One month later, all females were euthanized. Weights and lengths of ovaries and uterine horns were measured. Representative parts of the ovaries were subjected to histomorphometry analysis of folliculogenesis. Granulosa cells were isolated and cell viability, proliferation (accumulation of PCNA, cyclin B1, and BrdU-positive cells), apoptosis (accumulation of bax, caspase 3, and DNA fragmentation) were evaluated. Granulosa cells were subjected to proteomic analysis by using the nano HPLC-Chip-MS/MS method. Estradiol and progesterone release by ovarian and granulosa cells was assessed by ELISA. Ovarian and uterine horn weights were lower in RF than NF. The diameter of follicles and oocytes and the thickness of the theca and granulosa cells were higher in RF than NF. RF showed a lower percentage of cells containing bax and caspase 3, occurrence of DNA fragmented cells, and estradiol and progesterone. RF had higher incorporation of BrdU, a higher proportion of cells containing PCNA and cyclin B1, and a lower percentage of viable cells. RF produced more specific proteins than NF, including peptides involved in cell differentiation, proliferation/division, mitotic cell cycle, and GTP-ase activity. In conclusion, food restriction can activate reproduction by (1) selection of the growing primordial follicles, (2) better transformation of secondary to preovulatory follicles, (3) increasing growth of oocytes, (4) increasing proliferation and decreasing apoptosis in granulosa cells, (5) changes in ovarian secretory activity, and (6) changes in the number of peptides.","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 9","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12071153/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143966755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Forage Turnip (Brassica rapa L.) as a Dietary Supplement to Improve Meat Quality. 饲料萝卜作为饲料添加剂提高肉品质的研究。

IF 2.7 2区农林科学

Animals Pub Date : 2025-04-30 DOI: 10.3390/ani15091277

Romina Rodríguez-Pereira, Ignacio Subiabre, Cristian J Moscoso, Carolina E Realini, Rodrigo Morales

{"title":"Forage Turnip (Brassica rapa L.) as a Dietary Supplement to Improve Meat Quality.","authors":"Romina Rodríguez-Pereira, Ignacio Subiabre, Cristian J Moscoso, Carolina E Realini, Rodrigo Morales","doi":"10.3390/ani15091277","DOIUrl":"10.3390/ani15091277","url":null,"abstract":"Summer turnips (Brassica rapa L.) have become one of the main supplementary crops in ruminant livestock systems in southern Chile because of accelerated forage growth as well as greater forage yield and nutritive value in the dry season. This study investigated the effects of forage turnip supplementation on the physicochemical and sensory quality of beef from steers. Twenty-seven Holstein-Friesian steers were allocated to one of three dietary treatments: pasture plus concentrate (Control), 50% turnip with a basal diet of pasture hay and rolled corn (T50), and 70% turnip with the same basal diet (T70). Carcass yields and physicochemical and sensory beef attributes, including fatty acid composition of intramuscular fat (IMF) in lean tissue, were measured. Compared to the Control diet, finishing steers on 50% or 70% turnips increased meat redness (a* > 25.9 vs. 22.9 in Control), IMF (1.79% in T50 vs. 1.12% in Control), polyunsaturated fatty acids (PUFAs) (especially n-3), cholesterol, and specific minerals (sodium, manganese and iron); this resulted in a reduction in the subcutaneous fat thickness (0.29 cm in T50, 0.25 cm in T70 vs. 0.51 cm in Control) and shear force of cooked meat (p < 0.05). However, no differences were found between diets in beef juiciness, flavor, and tenderness assessed by trained panelists (p > 0.05). Increasing forage turnip inclusion to 70% resulted in similar beef quality to the 50% inclusion level. Foraged turnips present a promising strategy for producing high-quality beef during periods of limited pasture growth.","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 9","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12070835/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143973714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Animals最新文献