Animal Reproduction Science最新文献

Selenium yeast alleviates diquat-induced oxidative stress and testicular damage in roosters. 酵母硒可减轻diquat诱导的公鸡氧化应激和睾丸损伤。

IF 2.2 2区农林科学

Animal Reproduction Science Pub Date : 2025-02-01 Epub Date: 2024-12-28 DOI: 10.1016/j.anireprosci.2024.107760

Ningna Xiong, Sili Liu, Wei Hu, Yamei Liu, Xiang Ding, Deming Xu, Yiqiang Ling, Manxin Fang, Ben Liu, Lucheng Zheng, Wenya Zheng, Bingyan Wu

{"title":"Selenium yeast alleviates diquat-induced oxidative stress and testicular damage in roosters.","authors":"Ningna Xiong, Sili Liu, Wei Hu, Yamei Liu, Xiang Ding, Deming Xu, Yiqiang Ling, Manxin Fang, Ben Liu, Lucheng Zheng, Wenya Zheng, Bingyan Wu","doi":"10.1016/j.anireprosci.2024.107760","DOIUrl":"10.1016/j.anireprosci.2024.107760","url":null,"abstract":"Diquat (DQ) is a pro-oxidant that generates free radicals in cells through redox reactions, leading to the induction of oxidative stress. During the processes of growth and reproduction, poultry are particularly vulnerable to oxidative stress. Selenium yeast (SeY) serves as an organic selenium source characterized by high activity and low toxicity, imparting antioxidant effects. The objective of this study was to assess the protective effects of SeY against DQ-induced oxidative stress in rooster testicles.The results demonstrated that SeY pretreatment mitigated DQ-induced oxidative damage in the testes. This mitigation encompassed the alleviation of inhibited spermatogenesis, reduced spermatogenic cell abundance, and the alleviation of decreased expression of genes StAR, P450scc, and 3β-HSD which related to testosterone synthesis. Specifically, SeY pretreatment counteracted DQ-induced oxidative stress by activating the Nrf2/HO-1 antioxidant signaling pathway, enhancing the activity of antioxidant enzymes such as catalase (CAT) and total superoxide dismutase (T-SOD), and reducing the concentration of malondialdehyde (MDA). Furthermore, SeY pretreatment attenuated DQ-induced spermatogonia apoptosis by modulating the expression of apoptosis-related genes and proteins, including Bax, Bcl-2, Caspase3, and NF-κB. Additionally, SeY restored the proliferative capacity of spermatogenic cells by promoting the expression of the proliferation-related protein Ki67. The aforementioned findings signify that SeY effectively safeguards the testes against DQ-induced damage through mechanisms involving the reduction of oxidative stress, inhibition of apoptosis, promotion of proliferation, and enhancing the expression of testosterone synthesis related genes. This study lays a solid theoretical foundation for future research aimed at safeguarding the reproductive health of male poultry exposed to agricultural pesticides.","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"273 ","pages":"107760"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142930718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SUMO1 modification reduces oxidative stress and SUMO1ylated AKAP4 degradation affects frozen-thawed boar sperm quality. SUMO1修饰降低氧化应激，SUMO1化的AKAP4降解影响冻融猪精子质量。

IF 2.2 2区农林科学

Animal Reproduction Science Pub Date : 2025-02-01 Epub Date: 2024-12-27 DOI: 10.1016/j.anireprosci.2024.107759

Yuyang Zhang, Xuan Chen, Lipeng Cao, Junzheng Zhang, Jie Wang, Zhiwei Yao, Kun Zhao, Yi Jin

{"title":"SUMO1 modification reduces oxidative stress and SUMO1ylated AKAP4 degradation affects frozen-thawed boar sperm quality.","authors":"Yuyang Zhang, Xuan Chen, Lipeng Cao, Junzheng Zhang, Jie Wang, Zhiwei Yao, Kun Zhao, Yi Jin","doi":"10.1016/j.anireprosci.2024.107759","DOIUrl":"https://doi.org/10.1016/j.anireprosci.2024.107759","url":null,"abstract":"Low-temperature injury affects normal physiological function and viability of boar sperm during cryopreservation. Small ubiquitin-like modifier (SUMO) modification of proteins after translation is related to the cell stress response but the relationship between SUMO modification and oxidative stress in freeze-thawed sperm remains unclear. A-kinase ankyrin 4 (AKAP4) and its precursor proAKAP4 are two main proteins in mammalian sperm. Although AKAP4 expression has been studied in many species, its expression in porcine sperm has not been described in detail. In this study, liquid chromatography-mass spectrometry was used to determine the differentially expressed SUMO-modified proteins in porcine sperm after freezen and thawed. The results identified 26 down-regulated SUMO-modified proteins, with AKAP4 identified as one of the target proteins of SUMO1 under sperm stress. In addition, the level of SUMO1 protein increased significantly (P < 0.001) and the level of AKAP4 protein decreased (P < 0.05) after freezing and oxidative stress treatment. Inhibition of SUMO1 modification of AKAP4 protein did not affect its degradation (P > 0.05), indicating that SUMO1 is not involved in the degradation of AKAP4. The inhibition of SUMO1 modification by sperm protein decreased sperm motility (P < 0.05), ATP content, and DNA integrity (P < 0.05). In summary, cryopreservation and oxidative stress can induce SUMO modification of porcine sperm proteins and the modification of sperm protein SUMO1 can help sperm resist oxidative stress; and its role in protecting sperm quality is not via regulating the degradation of AKAP4. These findings provide new insights into the mechanisms underlying SUMO1 modifications during sperm cryopreservation and oxidative stress.","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"273 ","pages":"107759"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142998804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic imprinting and environmental epigenetics: Their influence on sheep reproductive traits across parities. 基因组印记和环境表观遗传学：基因组印记与环境表观遗传学：它们对绵羊不同性别繁殖特征的影响

IF 2.2 2区农林科学

Animal Reproduction Science Pub Date : 2025-02-01 Epub Date: 2024-12-09 DOI: 10.1016/j.anireprosci.2024.107668

Fatemeh Tavasolian, Mohsen Gholizadeh, Hasan Hafezian

{"title":"Genomic imprinting and environmental epigenetics: Their influence on sheep reproductive traits across parities.","authors":"Fatemeh Tavasolian, Mohsen Gholizadeh, Hasan Hafezian","doi":"10.1016/j.anireprosci.2024.107668","DOIUrl":"10.1016/j.anireprosci.2024.107668","url":null,"abstract":"This study aimed to explore the impact of genomic imprinting on the genetic variance of composite reproductive traits across three parities in Baluchi sheep. The traits analyzed included litter mean weight per lamb born (LMWLB), litter mean weight per lamb weaned (LMWLW), total litter weight at birth (TLWB), and total litter weight at weaning (TLWW). We employed a univariate linear animal model for each trait, treating performance across parities as separate traits. Twenty-four animal models were assessed, incorporating direct additive genetic effects, maternal genetic effects, maternal permanent environmental effects, direct and maternal genetic covariances, as well as maternal and paternal imprinting. Model selection was based on Akaike's Information Criterion (AIC). Direct heritability estimates for the traits were generally low, ranging from 0.039 ± 0.017 for LMWLW to 0.085 ± 0.028 for TLWW. TLWB and TLWW exhibited higher heritability than LMWLB and LMWLW in their respective parities. In the best model (model 24), maternal imprinting heritability estimates from the first to third parity were 0.059 ± 0.016, 0.060 ± 0.013, and 0.085 ± 0.021 for TLWB, 0.075 ± 0.021, 0.068 ± 0.025, and 0.048 ± 0.016 for LMWLB, 0.051 ± 0.013, 0.065 ± 0.019, and 0.068 ± 0.020 for TLWW, and, 0.072 ± 0.012, 0.057 ± 0.018 and 0.054 ± 0.011 for LMWLW, respectively. Paternal imprinting heritability estimates were consistently lower than maternal imprinting estimates, with values across parities ranging from 0.001 ± 0.024 to 0.019 ± 0.032 for TLWB, 0.005 ± 0.022-0.010 ± 0.019 for LMWLB, 0.012 ± 0.05-0.017 ± 0.05 for TLWW and, 0.013 ± 0.01-0.016 ± 0.01 for LMWLW. In conclusion, imprinting effects should be included in breeding programs to increase the accuracy of genetic evaluation.","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"273 ","pages":"107668"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142821639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Letter from the Guest Editor. 客座编辑的来信。

IF 2.2 2区农林科学

Animal Reproduction Science Pub Date : 2025-02-01 Epub Date: 2024-12-17 DOI: 10.1016/j.anireprosci.2024.107679

John Kastelic

引用次数: 0

Hormonal symphony: The dynamic duo of IGF and EGF in gonadotropin-induced fish ovarian development and egg maturation. 激素交响乐：促性腺激素诱导的鱼类卵巢发育和卵子成熟中IGF和EGF的动态二重奏。

IF 2.2 2区农林科学

Animal Reproduction Science Pub Date : 2025-02-01 Epub Date: 2024-12-05 DOI: 10.1016/j.anireprosci.2024.107663

Sourav Kundu, Basanta Kumar Das, Subhadeep Das Gupta

{"title":"Hormonal symphony: The dynamic duo of IGF and EGF in gonadotropin-induced fish ovarian development and egg maturation.","authors":"Sourav Kundu, Basanta Kumar Das, Subhadeep Das Gupta","doi":"10.1016/j.anireprosci.2024.107663","DOIUrl":"10.1016/j.anireprosci.2024.107663","url":null,"abstract":"Fish oocyte maturation (FOM) is a critical biological process that occurs before ovulation and is influenced by gonadotropins, particularly luteinizing hormone (LH). The release of LH stimulates the ovarian follicle to produce a maturation-inducing hormone (MIH), specifically 17α, 20β-dihydroxy-4-pregnen-3-one (17α, 20β-DP), which initiates the formation of maturation-promoting factor (MPF) through the activation of cyclin B and cdc2 kinase. Insulin-like growth factor I (IGF-I) significantly regulates ovarian functions, including steroidogenesis, by activating its membrane receptors and the tyrosine kinase pathway. IGF-I influences oocyte maturation directly via the PI3 kinase pathway, independent of steroid hormones. Additionally, epidermal growth factor (EGF) promotes cell growth and differentiation by binding to its receptor (EGFR). It is implicated in mediating human chorionic gonadotropin (hCG)-induced DNA synthesis in ovarian follicles while suppressing apoptosis. The presence of EGF in follicle cells and oocytes, along with its higher expression in oocytes, suggests it may act as a paracrine signal regulating somatic cell activity. Recent studies indicate that the activin system in follicle cells could be a target for EGF activity. The EGFR signaling pathway enhances gonadotropin-induced steroidogenesis and governs the transition of oocyte maturation stages, essential for successful fertilization. This review synthesizes current research on the roles of gonadotropins, IGFs, and EGFs in fish oocyte maturation and ovarian steroid production.","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"273 ","pages":"107663"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142823787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A review: Breeding behavior and management strategies for improving reproductive efficiency in bulls. 综述：提高公牛繁殖效率的育种行为及管理策略。

IF 2.2 2区农林科学

Animal Reproduction Science Pub Date : 2025-02-01 Epub Date: 2024-12-12 DOI: 10.1016/j.anireprosci.2024.107669

Jennifer M Pearson

{"title":"A review: Breeding behavior and management strategies for improving reproductive efficiency in bulls.","authors":"Jennifer M Pearson","doi":"10.1016/j.anireprosci.2024.107669","DOIUrl":"10.1016/j.anireprosci.2024.107669","url":null,"abstract":"This review focuses on bull breeding behaviors and management strategies to improve reproductive efficiency. Breeding soundness evaluations are utilized to classify a bull's physical ability and sperm quality, yet roughly 20 % of bulls fail to meet the minimum criteria. Furthermore, despite achieving the minimum criteria, few bulls in multi-sire breeding groups sire the majority of calves, indicating a need for better understanding of bull behavior that impact siring capacity, and thus, a bull's reproductive efficiency. Several factors influence bull libido such as age, breed, and environmental conditions. Although service capacity tests have been used to measure libido, standardization and repeatability, along with variability in age and breed, can be problematic. Management in collection facilities largely focuses on the pre-stimulation of bulls through behavioral cues for better sperm quality and quantity during collection, thus improving a bull's reproductive efficiency through fewer collections with increased breeding doses harvested. In management of multi-sire breeding groups, understanding social interactions, bull-to-female ratios, synchronization of females, and DNA testing to determine parentage, are techniques that can be utilized to improve reproductive efficiency. New research utilizing remote monitoring technology is being developed to better understand bull behavior without the constraints of direct observation. This technology may be used to predict siring capacity, better manage bulls based on social dynamics, and potentially detect lameness or injury in bulls that may impact siring capacity. A better understanding of developing management strategies of breeding behaviors should be further investigated to improve reproductive success of bulls.","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"273 ","pages":"107669"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142871156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hormonal induction and seasonal variation in male reproductive viability of the Southern Rocky Mountain boreal toad. 南落基山北方蟾蜍雄性生殖能力的激素诱导和季节变化。

IF 2.2 2区农林科学

Animal Reproduction Science Pub Date : 2025-02-01 Epub Date: 2024-12-14 DOI: 10.1016/j.anireprosci.2024.107678

Natalie E Calatayud, Leah Jacobs, Gina Della Togna, Cecilia J Langhorne, Amanda C Mullen, Rose Upton

{"title":"Hormonal induction and seasonal variation in male reproductive viability of the Southern Rocky Mountain boreal toad.","authors":"Natalie E Calatayud, Leah Jacobs, Gina Della Togna, Cecilia J Langhorne, Amanda C Mullen, Rose Upton","doi":"10.1016/j.anireprosci.2024.107678","DOIUrl":"10.1016/j.anireprosci.2024.107678","url":null,"abstract":"The Southern Rocky Mountain boreal toad (Anaxyrus boreas boreas) depends on both the rearing of wild-collected egg masses and a long-standing conservation breeding program (CBP), the latter of which heavily relies on assisted reproductive technologies (ARTs) to support wild populations. Achieving consistent reproductive success in the CBP, however, remains a significant challenge. Natural breeding has not led to a sustained increase in reproductive capacity, prompting the exploration of exogenous hormone treatments as an alternative strategy. This study specifically examined male responses to the administration of human chorionic gonadotropin (hCG), a previously tested hormone, in combination with gonadotropin-releasing hormone agonist (GnRH-A), either individually or together, to evaluate their effects on sperm induction and viability across different seasons. Insights into how hormone treatments and seasonality influence sperm acquisition can guide managers in improving breeding outcomes within ex situ populations by enhancing their understanding of reproductive health, applying hormone treatments at the optimal time of year, and determining the best timing for high quality sperm collection. These advancements can increase reproductive capacity and support long-term genetic management through biobanking. Results indicated that combining hCG and GnRH-A yielded the highest sperm quantity and quality, although further optimization of hormone dosages could improve outcomes. Seasonal factors significantly influenced hormonal efficacy, with variations in sperm concentration and quality observed across months.","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"273 ","pages":"107678"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142871157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Atractylenolide Ⅲ partially alleviates tunicamycin-induced damage in porcine oocytes during in vitro maturation by reducing oxidative stress. 白术内酯Ⅲ通过减少氧化应激，部分减轻了兔霉素在体外成熟过程中对猪卵母细胞的损伤。

IF 2.2 2区农林科学

Animal Reproduction Science Pub Date : 2025-02-01 Epub Date: 2024-12-29 DOI: 10.1016/j.anireprosci.2024.107761

Chuang Li, Butian Zhang, Minkyu Kim, Haixing Liu, Feiyang Yang, Ke Chen, Hongfeng Shi

{"title":"Atractylenolide Ⅲ partially alleviates tunicamycin-induced damage in porcine oocytes during in vitro maturation by reducing oxidative stress.","authors":"Chuang Li, Butian Zhang, Minkyu Kim, Haixing Liu, Feiyang Yang, Ke Chen, Hongfeng Shi","doi":"10.1016/j.anireprosci.2024.107761","DOIUrl":"https://doi.org/10.1016/j.anireprosci.2024.107761","url":null,"abstract":"Assisted reproductive technology (ART) is widely used to address infertility and enhance reproductive outcomes in livestock. Among various ART techniques, in vitro maturation (IVM) is commonly used to obtain high-quality oocytes but is susceptible to oxidative stress. In traditional Chinese medicine, Rhizoma Atractylodis Macrocephalae (Bai Zhu) is used to enhance maternal and fetal health. Atractylenolide Ⅲ (AⅢ), a major component of Bai Zhu, has shown both antioxidant properties and oxidative stress induction, leading to controversy. This study used porcine oocytes as a model to investigate the effects of AⅢ under tunicamycin (TM)-induced oxidative stress. During IVM, oocytes were treated with various concentrations of AⅢ and a constant dose of TM. AⅢ promoted oocyte maturation and cumulus cell expansion, with the optimal concentration being 1 mg/L. AⅢ reduced reactive oxygen species (ROS) and malondialdehyde (MDA) levels, indicating reduced oxidative damage. Mitochondrial function and membrane potential (MMP) were preserved in AⅢ-treated oocytes. Additionally, AIII could alleviate TM-induced endoplasmic reticulum (ER) stress, as shown by decreased mRNA expression of ER stress markers. Following parthenogenetic activation (PA), AⅢ-treated oocytes exhibited increased cleavage and blastocyst formation rates with reduced apoptosis compared to the TM group. These findings suggest that AⅢ protects against oxidative stress, improving oocyte quality and developmental potential, with potential applications in ART.","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"273 ","pages":"107761"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142998803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitochondria-targeted antioxidant MitoQ improves the quality of low temperature-preserved yak semen via alleviating oxidative stress. 线粒体靶向抗氧化剂MitoQ通过减轻氧化应激改善低温保存牦牛精液的质量。

IF 2.2 2区农林科学

Animal Reproduction Science Pub Date : 2025-02-01 Epub Date: 2024-12-19 DOI: 10.1016/j.anireprosci.2024.107680

Yanjin Zhu, Jun Yu, Qinhui Yang, Yumian Xie, Xupeng Li, Zhuo Chen, Yan Xiong, Wei Fu, Honghong He, Shi Yin, Daoliang Lan, Jian Li, Xianrong Xiong

{"title":"Mitochondria-targeted antioxidant MitoQ improves the quality of low temperature-preserved yak semen via alleviating oxidative stress.","authors":"Yanjin Zhu, Jun Yu, Qinhui Yang, Yumian Xie, Xupeng Li, Zhuo Chen, Yan Xiong, Wei Fu, Honghong He, Shi Yin, Daoliang Lan, Jian Li, Xianrong Xiong","doi":"10.1016/j.anireprosci.2024.107680","DOIUrl":"10.1016/j.anireprosci.2024.107680","url":null,"abstract":"Low-temperature preservation of yak semen during transportation and conservation is crucial to accelerate yak breeding. The effects of low-temperature cooling on yak semen quality, however, are poorly understood. This study aimed to determine the dose-dependent effect of mitochondria-targeted antioxidant \"MitoQ\" on the motility, oxidative status, and mitochondrial function of yak semen during low-temperature preservation. Semen samples were collected from six adult healthy Maiwa yaks and preserved at 4 ℃ in semen extender containing 0, 50, 100, 200, and 400 nM MitoQ, respectively. Firstly, the motility, membrane integrity, acrosome integrity, and abnormity index of yak spermatozoa were evaluated to determine the optimal MitoQ concentration. Next, the effect of MitoQ at the optimal concentration on spermatozoa antioxidant capacity, including reactive oxygen species (ROS) and malondialdehyde (MDA) contents, total antioxidant capacity (T-AOC), and superoxide dismutase content (SOD) levels, as well as mitochondrial membrane potential were analyzed. Up to 96 h of low-temperature storage, 200 nM MitoQ showed the most optimal effect on motility, membrane integrity, and acrosome integrity (P < 0.05) but not on sperm morphology (P > 0.05). Also, 200 nM MitoQ markedly reduced yak spermatozoa ROS and MDA contents for up to 48 h of low-temperature storage (P < 0.05). Finally, 200 nM MitoQ significantly improved T-AOC, SOD, and mitochondrial membrane potential for up to 24, 48, and 72 h of low-temperature storage, respectively (P < 0.05). In summary, semen extender supplementation with 200 nM MitoQ is beneficial for low-temperature yak semen preservation via improving the oxidative status.","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"273 ","pages":"107680"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142875806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of MFN2 in bovine embryonic development and the mitigation of ER stress. MFN2在牛胚胎发育和内质网应激中的作用。

IF 2.2 2区农林科学

Animal Reproduction Science Pub Date : 2025-02-01 Epub Date: 2024-12-04 DOI: 10.1016/j.anireprosci.2024.107664

Shu-Ming Shi, Bing Hu, Zhi-Chao Chi, Lin-Yi Qu, Li-Ying Liu, Yu-Yan He, Guan-Lin Jia, Jing-Hang Li, Ilkeun Kong, Yong-Xun Jin, Xian-Feng Yu

{"title":"Role of MFN2 in bovine embryonic development and the mitigation of ER stress.","authors":"Shu-Ming Shi, Bing Hu, Zhi-Chao Chi, Lin-Yi Qu, Li-Ying Liu, Yu-Yan He, Guan-Lin Jia, Jing-Hang Li, Ilkeun Kong, Yong-Xun Jin, Xian-Feng Yu","doi":"10.1016/j.anireprosci.2024.107664","DOIUrl":"10.1016/j.anireprosci.2024.107664","url":null,"abstract":"This study investigated the role of mitochondrial fusion protein-2 (MFN2) in bovine embryonic development and its relationship with endoplasmic reticulum (ER) stress, aiming to increase the efficiency of in vitro embryo culture. Western blot analysis revealed that MFN2 expression peaked at the 2-cell stage, decreased at the 4-cell stage, and gradually increased from the 6-8-cell stage to the blastocyst stage. Inhibiting MFN2 at the zygote stage reduced blastocyst formation and proliferation, and this damage was partially reversed by the ER stress protective agent TUDCA. MFN2 inhibition also led to the decreased formation of the inner cell mass (ICM) and reduced expression of the totipotent genes CDX2 and SOX2. Additionally, reactive oxygen species (ROS) levels increased following MFN2 inhibition but decreased after TUDCA treatment. The expression of antioxidative stress-related genes (SOD and CAT) was downregulated after MFN2 inhibition but upregulated following TUDCA treatment. Furthermore, MFN2 inhibition reduced ER fluorescence intensity and increased the expression of UPR signaling markers (GRP78, XBP1, CHOP, IRE1, and ATF6), indicating increased ER stress. TUDCA administration reversed these effects, restoring MFN2 levels and reducing apoptosis. In conclusion, MFN2 is essential for bovine embryonic development because it regulates ER stress and maintains cell function, with MFN2 deficiency leading to developmental disorders and cell damage. ER stress protectors such as TUDCA can effectively mitigate these negative effects, highlighting a potential strategy for improving in vitro embryo culture efficiency.","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"273 ","pages":"107664"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142827214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0