Animal Reproduction Science最新文献

{"title":"The combination of FLI, ITS, pFF, and citrate improves the developmental potential of gilt oocytes during in vitro maturation","authors":"Zhilong Chen ,&nbsp;Caiyong Zhang ,&nbsp;Chao Yan ,&nbsp;Cuiting Peng ,&nbsp;Hao Xie ,&nbsp;Yingting Pan ,&nbsp;Lin Qi ,&nbsp;Chaoqun Qu ,&nbsp;Yulan Zhao ,&nbsp;Zhonglin Tang","doi":"10.1016/j.anireprosci.2025.107945","DOIUrl":"10.1016/j.anireprosci.2025.107945","url":null,"abstract":"<div><div>The intrinsic quality of porcine oocytes is a critical determinant of early embryonic development during <em>in vitro</em> production (IVP). This study investigated the effects of chemically defined <em>in vitro</em> maturation (IVM) systems supplemented with glutamine, GlutaMAX, insulin-transferrin-selenium (ITS), porcine follicular fluid (pFF), citrate, and growth factors (FGF2, LIF, IGF1, termed FLI) on the cumulus diffusion and maturation of oocytes derived from different diameters follicles. Moreover, four IVM media were systematically assessed for their effects on oocyte nuclear and cytoplasmic maturation and subsequent embryonic development. Results showed that the addition of pFF or FLI alone significantly enhanced cumulus expansion (<em>P</em> &lt; 0.05). FLI medium combined with ITS, citrate, and pFF, exhibited higher average cumulus diffusion diameters and rates of first polar body extrusion. Notably, the synergistic combination of ITS, citrate, and pFF with FLI medium upregulated expression of cumulus expansion makers (<em>HAS2</em>, <em>PTX3</em>, <em>CX43</em>, <em>TNFAIP6</em>) and oocyte maturation makers (<em>GDF9</em> and <em>BMP15</em>), improved cortical granule distribution, spindle morphology, chromosome alignment, and elevated glutathione (GSH) levels. This combination also enhanced cleavage and blastocyst rates, increased inner cell mass cell counts in blastocysts, and improved enucleation and fusion efficiency of oocytes following somatic cell nuclear transfer (SCNT). Collectively, the combination of FLI medium with ITS, pFF, and citrate synergistically promoted the maturation and developmental potential of porcine oocytes derived from prepubertal gilts, including those from small follicles. This optimized IVM system provides a robust foundation for advancing porcine IVP in reproductive biotechnology.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"279 ","pages":"Article 107945"},"PeriodicalIF":2.2,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144320827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Age-related changes in semen characteristics of selected Slovak geese breeds","authors":"Eva Tvrdá ,&nbsp;Cyril Hrnčár ,&nbsp;Filip Benko ,&nbsp;Michal Ďuračka ,&nbsp;Anton Kováčik ,&nbsp;Jakub Vozaf ,&nbsp;Andrea Svoradová ,&nbsp;Henrieta Arpášová ,&nbsp;Miroslava Kačániová ,&nbsp;Peter Chrenek","doi":"10.1016/j.anireprosci.2025.107888","DOIUrl":"10.1016/j.anireprosci.2025.107888","url":null,"abstract":"<div><div>This paper strove to characterize and compare ejaculates collected from two geese breeds over the period of two years in the context of selected traditional, biochemical, oxidative, bacteriological, and immunological properties. Slovak white and Suchovy ganders were selected for sperm quality evaluation, while bacteria were characterized using the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Oxidative profile of semen was assessed using chemiluminescent and colorimetric protocols. Levels of pro-inflammatory cytokines and antibacterial proteins were evaluated with the enzyme-linked immunosorbent assay. Seminal plasma biochemistry was assessed with an automated clinical chemistry analyzer. Overall, lower semen quality was found in the Slovak white breed and during the first year of semen collection in both breeds. At the same time, the sperm motility was in a strong positive correlation with the plasma membrane integrity (<em>P</em> &lt; 0.0001), whilst being negatively associated with the bacterial load (<em>P</em> = 0.0056), reactive oxygen species production (<em>P</em> &lt; 0.0001), DNA fragmentation (<em>P</em> &lt; 0.0001) and the levels of interleukin-1 (<em>P</em> &lt; 0.0001) and C-reactive protein (<em>P</em> &lt; 0.0001). Higher semen quality in ganders was accompanied by increased seminal plasma levels of lysozyme (<em>P</em> &lt; 0.0001), cathelicidin (<em>P</em> &lt; 0.0001), calcium (<em>P</em> &lt; 0.01), magnesium (<em>P</em> &lt; 0.01 in the case of the Suchovy breed), phosphorus (<em>P</em> &lt; 0.001), total proteins (<em>P</em> &lt; 0.0001 with respect to the Slovak white breed; <em>P</em> &lt; 0.01 with regards to the Suchovy breed) and cholesterol (<em>P</em> &lt; 0.05), and a decreased activity of aspartate transaminase (<em>P</em> &lt; 0.0001). We may conclude that semen of overall better quality was obtained during the second year of semen collection as opposed to the first year in ganders of both selected goose breeds.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"279 ","pages":"Article 107888"},"PeriodicalIF":2.2,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144279624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An analysis addressing factors affecting risk of ovulation, pregnancy, and pregnancy loss in lactating dairy cows submitted to fixed-time artificial insemination programs with special reference to the diameter of the ovarian follicle","authors":"Jeffrey S. Stevenson","doi":"10.1016/j.anireprosci.2025.107887","DOIUrl":"10.1016/j.anireprosci.2025.107887","url":null,"abstract":"<div><div>Because reduced fertility in studies resulted from GnRH-induced ovulation of small or large follicles in lactating dairy cattle, the objective was to determine the association of follicle diameter and risks of ovulation and pregnancy per artificial insemination (P/AI). An analysis was conducted of data from 14 studies in 82 herd-year-seasons in which ovulation was synchronized before a fixed-time AI. Follicle diameter was included as a continuous variable in a random-effects mixed model that used herd-year-season as a random surrogate for study. Effects of presynchronization, parity, cycling status, body condition score, occurrence of estrus, service sire, and progesterone were included as appropriate in initial models plus all two-way interactions. Ovulation risk after first GnRH treatment of Ovsynch increased (<em>P</em> &lt; 0.001) with increasing follicle diameter. Ovulation risk after the breeding GnRH treatment increased (<em>P</em> = 0.03) in multiparous but not in primiparous cows as follicle diameter increased. Pregnancy per AI at d 28–37 and at d 56–84 was not associated with follicle diameter but was affected by presynchronization treatment (<em>P</em> = 0.03), ovulation after the breeding GnRH treatment (<em>P</em> = 0.003), parity (<em>P</em> &lt; 0.001), prebreeding cycling status (<em>P</em> &lt; 0.001), and progesterone (<em>P</em> &lt; 0.001) before AI. As follicle diameter before AI increased, pregnancy loss by d 56–84 after AI decreased (<em>P</em> = 0.006), especially in cows of greater body condition. These results do not support any effect of follicle size on P/AI in cows whose ovulation is synchronized before AI, but less pregnancy loss was associated with increasing follicle diameter.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"279 ","pages":"Article 107887"},"PeriodicalIF":2.2,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144307760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Schisandrin B mitigates oxidative damage in early porcine embryos exposed to Ochratoxin A via NRF2 pathway activation","authors":"Chuang Li ,&nbsp;Kuk Bin Ji ,&nbsp;Eun Young Kim ,&nbsp;Ho Yong Choi ,&nbsp;Jae Hyeok Yoon ,&nbsp;Xiang Zhang ,&nbsp;Ryoung Eun Kim ,&nbsp;Kang Sun Park ,&nbsp;Haixing Liu ,&nbsp;Min Kyu Kim","doi":"10.1016/j.anireprosci.2025.107889","DOIUrl":"10.1016/j.anireprosci.2025.107889","url":null,"abstract":"<div><div>Ochratoxin A (OTA), a mycotoxin produced by Aspergillus and Penicillium species, exerts toxic effects on reproduction and embryonic development through oxidative stress. Although OTA contamination in animal feed is a well-recognized threat, effective protective strategies remain limited. Schisandrin B (Sch-B), a bioactive lignan derived from Schisandra chinensis, is known for its potent antioxidant activity. This study investigated whether Sch-B could alleviate OTA-induced oxidative damage in porcine preimplantation embryos. Porcine zygotes were cultured in vitro with OTA (0.5 mg/L) and/or Sch-B (1 mg/L), and developmental competence, oxidative stress, mitochondrial function, apoptosis, and NRF2/KEAP1 signaling were assessed. OTA exposure significantly reduced cleavage and blastocyst formation rates, increased ROS and MDA levels, and impaired mitochondrial membrane potential. In contrast, Sch-B treatment restored embryonic development, decreased ROS accumulation, increased glutathione (GSH) levels, and improved mitochondrial function. Moreover, Sch-B upregulated NRF2 expression and downregulated KEAP1, indicating activation of the NRF2-mediated antioxidant pathway. Apoptosis assays and mRNA analysis revealed that Sch-B attenuated OTA-induced cell death by modulating the expression of pro- and anti-apoptotic genes. Co-treatment, however, did not fully restore all parameters to control levels, suggesting that higher Sch-B concentrations or additional interventions may be needed. These results demonstrate that Sch-B mitigates OTA-induced oxidative stress and supports early embryonic development via NRF2 pathway activation. Sch-B represents a promising candidate for improving in vitro embryo culture conditions and counteracting mycotoxin-induced reproductive toxicity.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"279 ","pages":"Article 107889"},"PeriodicalIF":2.2,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144297019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of resveratrol supplementation in conventional slow freezing and kinetic vitrification media on the post-cryopreserved quality of dog epididymal spermatozoa","authors":"Diego A. Galarza ,&nbsp;Mauricio Duma ,&nbsp;Karla Carreño-Aguilar ,&nbsp;José Sañay-Sañay ,&nbsp;Cristina Bernardi ,&nbsp;Manuel E. Soria ,&nbsp;María S. Méndez","doi":"10.1016/j.anireprosci.2025.107886","DOIUrl":"10.1016/j.anireprosci.2025.107886","url":null,"abstract":"<div><div>Resveratrol (RES) is a powerful antioxidant that has recently been shown to improve sperm survival subjected to different cryopreservation methods. This study evaluated the effect of RES supplementation in conventional slow (CS) freezing and kinetic vitrification (VIT) media on dog sperm quality variables. Twenty pooled sperm samples from 30 orchiectomized adult dogs were used (three epididymal samples / pool). In each pooled sample, four treatments were established based on RES supplementation (control <em>[no added]</em> or 100 µM) and the cryopreservation method (<em>CS</em> or <em>VIT</em>): CS-Control, CS+RES, VIT-Control and VIT+RES. The CS freezing was performed by exposing sperm samples in 0.25 ml straws to liquid nitrogen (LN₂) vapors. Vitrification was made by directly submerging 30 µl sperm droplets into LN₂. The results initially demonstrated that VIT treatments produced outcomes similar to those of CS freezing in terms of sperm kinematics and membrane integrity. Sperm samples from the CS+RES treatment showed higher percentages (P &lt; 0.05) of motility, viability, acrosomal integrity, and DNA integrity compared to those from the CS-Control treatment. Furthermore, both CS+RES and VIT+RES treatments obtained a lower percentage (P &lt; 0.05) of sperm DNA fragmentation compared to both CS-Control and VIT-Control treatments. Despite this, RES negatively affected certain kinematic parameters (e.g., velocities, straightness, and beat cross frequency) and sperm head dimensions after vitrification. In conclusion, resveratrol improved sperm motility, viability, and acrosomal integrity and reduced DNA fragmentation following conventional slow freezing. While it also reduced DNA fragmentation after vitrification, it negatively affected sperm kinematics in dog epididymal sperm.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"279 ","pages":"Article 107886"},"PeriodicalIF":2.2,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144242427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of semen collection methods on sperm quality and metabolite profile in goat seminal plasma: Comparing between artificial vagina and electro-ejaculator techniques","authors":"Chunrong Lv ,&nbsp;Allai Larbi ,&nbsp;Jiachong Liang ,&nbsp;Chunyan Li ,&nbsp;Badaoui Bouabid ,&nbsp;Guoquan Wu ,&nbsp;Guobo Quan","doi":"10.1016/j.anireprosci.2025.107885","DOIUrl":"10.1016/j.anireprosci.2025.107885","url":null,"abstract":"<div><div>This study compared two semen collection methods—artificial vagina (AV) and electro-ejaculator (EE)—to evaluate their effects on sperm quality and the metabolomic profile of goat seminal plasma (SP). Semen samples were analyzed for sperm characteristics, and SP was assessed using ultra-performance liquid chromatography-electrospray ionization quadrupole Orbitrap mass spectrometry. Sperm collected via AV showed lower ejaculate volume but significantly higher concentration and motility than EE. Metabolomic profiling revealed 568 and 350 metabolites in positive and negative ion modes, respectively, with 105 and 77 showing significant differential expression. In the negative ion mode, metabolites such as bezafibrate, phosphatidylinositol 16 and 18, and benzothiazoline were down-regulated in AV, while docosahexaenoic acid, arachidonic acid, phosphatidylcholine, rutin, pyruvate, famotidine, and citrate were up-regulated. In the positive ion mode, metabolites including astaxanthin, lapachol, and 3-acetylaconitine were down-regulated, while mannitol, choline, L-carnitine, and indirubin were up-regulated in AV. KEGG pathway enrichment showed 30 significantly altered pathways. This is the first study to assess the impact of AV and EE on the metabolomic profile of goat SP. The identified metabolites serve as promising biomarkers for evaluating sperm quality and assessing reproductive potential, with implications for improving semen collection techniques and conserving genetic resources.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"279 ","pages":"Article 107885"},"PeriodicalIF":2.2,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144242426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miR-1307/BRCA1 axis mediated oxidative stress promotes apoptosis of sow ovarian granulosa cells","authors":"Siqi Wang ,&nbsp;Jian Li ,&nbsp;Xing Du ,&nbsp;Lifan Zhang ,&nbsp;Shaoxian Cao ,&nbsp;Qifa Li","doi":"10.1016/j.anireprosci.2025.107884","DOIUrl":"10.1016/j.anireprosci.2025.107884","url":null,"abstract":"<div><div>Breast cancer associated gene 1 (BRCA1) mutation and consequential reduction in transcription cause cancer, predominantly in the breast and ovarian tissues. However, its noncancer-related functions in ovarian tissues remain largely unknown. Here, we report BRCA1 is strongly involved in sow granulosa cell (sGC) apoptosis and cycle, and is targeted and inhibited by the pro-apoptotic miR-1307. BRCA1 is highly abundant in sGCs, and up-regulated during sow follicular development, but weakly expressed in atretic follicles, indicating a close correlation with sow follicular development and atresia. A loss-of-function assay showed that BRCA1 suppresses sGC apoptosis and promotes cell cycle progression. Furthermore, miR-1307 is a pro-apoptotic factor that inhibits BRCA1 by targeting the 3′UTR of the latter. In addition, the miR-1307 and BRCA1 axis can be activated by oxidative stress (OS), which is related to the transcriptional activation of miR-1307 by FoxO3, a transcription factor activated by OS. In conclusion, this study provides a basis for understanding the role of BRCA1 in sow reproduction and a theoretical target for improving sow fertility.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"279 ","pages":"Article 107884"},"PeriodicalIF":2.2,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144230487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seminal plasma MicroRNA dynamics in stallion semen due to progressive motility and conception success","authors":"Hüseyin Özkan ,&nbsp;Kemal Tuna Olğaç ,&nbsp;Hasan Hüseyin Keçeli ,&nbsp;Murat Onur Yazlık ,&nbsp;Ufuk Kaya ,&nbsp;Mehmet Borga Tırpan ,&nbsp;Ergun Akçay","doi":"10.1016/j.anireprosci.2025.107876","DOIUrl":"10.1016/j.anireprosci.2025.107876","url":null,"abstract":"<div><div>This study aims to investigate the expression patterns of miR-34b, miR-122, let-7a in seminal plasma of stallions in relation to progressive motility and conception success, and to evaluate their potential as biomarkers. Fifteen adult stallions were enrolled in study. One ml of seminal plasma was kept at −80 °C, 2 ml of fresh semen was sampled from each stallion for sperm analysis, and the rest were used for artificial insemination only for one mare. Two groups were formed as high progressive motility (HPM; n:8) and low progressive motility (LPM; n:7), based on progressive motility. Besides, two groups were formed as positive (n:8) and negative (n:7) pregnancy groups according to 14th day pregnancy results. Motility and progressive motility values determined by computer-assisted semen analyzer. Flow cytometry was used to evaluate the viability, HMMP, PMAI, non-capacitated sperm rate and LPO parameters. MicroRNAs were evaluated with qPCR. Prediction of targets, protein-protein interactions and functional enrichment analyses were also performed. Total (82.13 ± 3.04; p &lt; 0.01) and progressive (45.88 ± 6.03; p &lt; 0.001) motilities, and non-capacitated sperm rates (45.76 ± 3.92; p &lt; 0.05) were greater in HPM. Compared to LPM, miR-34b (p &lt; 0.05) and miR-122 (p &lt; 0.05) were upregulated in HPM approximately 11- and 6-fold, respectively. miR-34b and total motility were positively correlated (0.674; p &lt; 0.01). Target gene analysis revealed a network of 102 genes and 421 edges. Moreover, significant interaction networks involving 19 proteins were determined. Performed enrichment analysis showed selected miRNAs significantly regulate crucial pathways. Regulatory differences of miR-34b and miR-122 in seminal plasma might serve to determine the characteristics of stallion semen.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"278 ","pages":"Article 107876"},"PeriodicalIF":2.2,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144167060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing gene editing in pigs: The role of electroporation and lipofection","authors":"Celia Piñeiro-Silva ,&nbsp;Joaquín Gadea","doi":"10.1016/j.anireprosci.2025.107874","DOIUrl":"10.1016/j.anireprosci.2025.107874","url":null,"abstract":"<div><div>The production of genetically modified pigs is becoming increasingly important in both the agricultural and biomedical fields. Optimization of these processes is a key objective to improve the precision, scalability and viability of genetically modified animals for research and commercial applications. Among the available techniques, electroporation and lipofection have emerged as promising alternatives to traditional methods such as microinjection and somatic cell nuclear transfer (SCNT) due to their simplicity, cost-effectiveness, and potential for high-throughput applications. These methods allow the direct delivery of CRISPR/Cas components into zygotes and embryos, reducing the technical expertise required and bypassing some of the challenges associated with cloning. This review examines the application, efficacy, and outcomes of electroporation and lipofection as gene editing techniques in porcine gametes and embryos. We provide a comprehensive synthesis of recent advances, compare their efficacy, and discuss their potential in agricultural and biomedical research. The principles and mechanisms of both methods are reviewed, highlighting their advantages, such as cost-effectiveness and ease of implementation, over traditional approaches such as microinjection. In addition, we address their limitations, including variability in efficiency, and discuss recent protocol optimizations aimed at improving reproducibility and applicability. By analyzing these developments, this review provides valuable insights into the evolving role of electroporation and lipofection in porcine genetic modification strategies.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"278 ","pages":"Article 107874"},"PeriodicalIF":2.2,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144178854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitochondrial bioenergetics analysis on SLC-selected boar spermatozoa during liquid storage","authors":"Estibaliz Lacalle ,&nbsp;Beatrice Mislei ,&nbsp;Cristina Algieri ,&nbsp;Marcella Spinaci ,&nbsp;Felipe Martinez Pastor ,&nbsp;Salvatore Nesci ,&nbsp;Jane M. Morrell ,&nbsp;Jose M. Ortiz-Rodriguez ,&nbsp;Diego Bucci","doi":"10.1016/j.anireprosci.2025.107875","DOIUrl":"10.1016/j.anireprosci.2025.107875","url":null,"abstract":"<div><div>Semen preservation at 17 °C plays a pivotal role in the porcine breeding industry, though it presents challenges; not all ejaculates retain their quality during storage. Colloidal centrifugation has been developed to improve it since this method eliminates seminal plasma and microorganisms while simultaneously allowing the selection of spermatozoa based on gradient density centrifugation. This study aimed to evaluate whether two different colloid densities (1.026 g/ml – 20 %Porcicoll® and 1.104 g/ml – 80 % Porcicoll®) improve sperm bioenergetics, assessed using Agilent seahorse, along with key semen quality parameters such as viability, mitochondrial activity, oxidative stress and motility analyzed through flow cytometry and the CASA system over time. Fifteen ejaculates were divided into three groups: Control (CTR), 20 % Porcicoll® (P20), and 80 % Porcicoll® (P80). Sperm parameters were analyzed at days 0, 3, and 7 of storage. Results revealed a metabolic shift from mitochondrial oxidative phosphorylation to glycolysis during storage, with a 15 % decrease in ATP production by day 3. P80-treated sperm showed higher ATP production on day 0 compared to P20. Sperm quality parameters such as viability and mitochondrial activity decreased after colloidal centrifugation, possibly because of early capacitation; we also hypothesize that SLC could increase sensitivity to the high glucose levels in the medium. Although Porcicoll® effectively selected metabolically active sperm, the lack of seminal plasma and limitations in media composition negatively affected long-term sperm quality. Further research is necessary to optimize media formulations and assess fertility outcomes of selected sperm for enhanced sperm preservation techniques.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"278 ","pages":"Article 107875"},"PeriodicalIF":2.2,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144185636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}