Animal Reproduction Science最新文献

{"title":"Superovulation treatment of immature female rabbits increases the number of ovulated oocytes that can in vitro develop into blastocytes.","authors":"Toshiaki Akiyoshi, Fumikazu Matsuhisa, Masashi Shiomi, Masatoshi Morimoto, Shuji Kitajima","doi":"10.1016/j.anireprosci.2025.107765","DOIUrl":"https://doi.org/10.1016/j.anireprosci.2025.107765","url":null,"abstract":"<p><p>To clarify the efficiency of superovulation in immature female rabbits, immature female rabbits were superovulated with pregnant mare serum gonadotropin, and the number of recovered oocytes, their maturity, and their ability to develop into blastocysts under in vitro fertilization and culture were examined in this study. More than 80 oocytes were recovered from 12-14-week-old immature female rabbits. In particular, an average of more than 100 oocytes were recovered from 13-week-old immature female rabbits. The number of oocytes in immature female rabbits was significantly-approximately 4 times-higher than in mature female rabbits. To compare oocyte maturity, oocytes from immature and mature female rabbits were compared by brilliant cresyl blue staining and mitochondrial distribution analysis. The proportion of mature oocytes detected by brilliant cresyl blue staining was higher in oocytes from mature female rabbits than in oocytes from immature female rabbits, but there were no significant differences in active mitochondrial distribution, fertilization rate, or blastocyst development rate. These results indicate that superovulation with immature female rabbits may be a useful technique for the collection of many oocytes.</p>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"274 ","pages":"107765"},"PeriodicalIF":2.2,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142998806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of luteal blood perfusion and expression of estrus on pregnancy rates of Bos taurus embryo recipients exposed to fixed-time embryo transfer.","authors":"Lucas Melo Goncalves, Samir Burato, Madison Blake Walker, Molly Smith, Lucas Neira, Gregory Clements, Shane Morgan, Reinaldo Fernandes Cooke, Pedro Levy Piza Fontes","doi":"10.1016/j.anireprosci.2025.107764","DOIUrl":"https://doi.org/10.1016/j.anireprosci.2025.107764","url":null,"abstract":"<p><p>This study evaluated the impact of luteal blood perfusion (BP) and expression of estrus on pregnancy rates of Bos taurus embryo recipients exposed to fixed-timed embryo transfer (FTET) using a gonadotropin-releasing hormone (GnRH)- and progesterone-based ovulation synchronization protocol. Postpartum beef cows (n = 746) were exposed to a GnRH/progesterone-based ovulation synchronization protocol. Luteal morphometry and BP were assessed using color Doppler ultrasonography 9 days after CIDR removal concurrently with FTET. Embryo recipients were retrospectively categorized according to CL area [small (< 2.5 cm²), medium (2.5-3.5 cm²), or large (> 3.5 cm²)] and BP [low (≤ 30 %), medium (>30 and ≤ 40 %), or high (> 40 % of luteal area with BP signals)]. Luteal area classification did not impact pregnancy rates (P = 0.50); however, cows with high luteal BP had greater (P < 0.01) pregnancy rates compared with low BP cows and tended to have greater pregnancy rates compared with medium BP cows (P = 0.07). Embryo recipients that expressed behavioral estrus had larger (P < 0.01) and more vascularized CL (P = 0.02), as well as greater (P = 0.02) pregnancy rates compared with females that did not express estrus. Nevertheless, no interaction between expression of behavioral estrus and luteal BP classification was observed (P = 0.41). In summary, luteal BP at FTET and expression of behavioral estrus were positively associated with pregnancy rates in Bos taurus embryo recipients exposed to progesterone- and GnRH-based protocols.</p>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"274 ","pages":"107764"},"PeriodicalIF":2.2,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142998805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relative increase in walking activity in lactating dairy cows submitted to timed artificial insemination: Risk factors and subsequent fertility.","authors":"E Rojas Canadas, I García-Ispierto","doi":"10.1016/j.anireprosci.2025.107762","DOIUrl":"https://doi.org/10.1016/j.anireprosci.2025.107762","url":null,"abstract":"<p><p>The objectives of this study were: (i) to evaluate the associations between postpartum health disorders, relative increase in walking activity (RIWA) and expression of behavioral estrus intensity captured by pedometers; (ii) to assess associations between RIWA and pregnancy in cows submitted to timed artificial insemination (TAI). Lactating dairy cows (n = 881) were enrolled in the study. Cows were submitted to a 5d TAI protocol with insertion of a progesterone device. Pregnancy status was determined by ultrasonography on D30 after AI. During the postpartum period cows were categorized as healthy (n = 468), having a reproductive disorder (n = 185), metabolic disorder (n = 118), reproductive and metabolic disorders (n = 56), or clinical mastitis (n = 54). Healthy cows tended to have (P = 0.10) a greater RIWA and a lower proportion of healthy cows did not exhibit estrus (P = 0.03) compared with cows that had a postpartum health disorder. Similarly, ovarian status at TAI protocol commencement (P = 0.01), presence of twin pregnancy (P = 0.03), and season (P < 0.0001) were all associated with RIWA. Cows with a corpus luteum (CL) at TAI protocol commencement, carrying twins and those served during the cold season had an enhanced RIWA compared with those with no CL, carrying singletons and cows served during the hot season. In addition, healthy cows (P = 0.003) and cows showing estrus (P = 0.0002) had enhanced pregnancy per TAI compared with those with postpartum disorders, and those failing to exhibit estrus, respectively. Hence, cows that remained healthy during the postpartum had a greater RIWA, expression of behavioral estrus intensity and pregnancy to TAI compared with those that had a postpartum disorder.</p>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"274 ","pages":"107762"},"PeriodicalIF":2.2,"publicationDate":"2025-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142969440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gonadal expression profiles reveal the underlying mechanisms of temperature effects on sex determination in the large-scale loach (Paramisgurnus dabryanus).","authors":"Lei Zhang, Yutong Fang, Mijuan Shi, Keyi Ren, Xin Guan, Waqar Younas, Yingyin Cheng, Wanting Zhang, Yaping Wang, Xiao-Qin Xia","doi":"10.1016/j.anireprosci.2024.107661","DOIUrl":"10.1016/j.anireprosci.2024.107661","url":null,"abstract":"<p><p>The sex determination mechanism in large-scale loach (Paramisgurnus dabryanus) follows a ZZ/ZW system, with sexual differentiation regulated by both genotypic factors and temperature effects (GSD+TSD), where elevated temperatures result in a higher proportion of males. Currently, research on the sex determination mechanisms in large-scale loach is limited, and the specific gene expression profiles and the role of temperature in influencing sex remain largely unknown. This study investigated the impact of temperature on the sex ratio in cultured populations of the large-scale loach, and then identified a female-specific genetic marker by whole genome sequencing, facilitating the distinguishing of females, males, and pseudo-males within this population. Transcriptomic analysis was subsequently performed on these groups, and the data revealed a similar expression pattern between pseudo-males and true-males. The research combined differential expression analysis with WGCNA to construct a regulatory network of nine sex differentiation-related genes (SDG) (map3k4, trpv4, hsd17b12a, wt1, ar, dmrt1, bcar1, sox9a, cyp17a1), indicating that sex differentiation in large-scale loach is probably driven by the regulation of male-related genes. The transcriptomic analysis suggested that temperature significantly modified the expression of SDG in the ovaries, while in the testes, it predominantly affects metabolism-related pathways. We established a temperature-sensitive gene network in females, based on the correlation between gene expression and temperature, as well as the number of co-regulated genes in female data. We propose that, with increasing temperature, wt1 serves as a central regulator, leading to the down-regulation of foxl2a, cyp19a1a, and the cholesterol biosynthesis-related gene sqlea, ultimately resulting in the development of pseudo-males.</p>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"272 ","pages":"107661"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142791012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pre-conception nutrition estimated from milk composition characterises the sex ratio of offspring in cattle.","authors":"Janez Belšak, Janez Jeretina, Maja Prevolnik Povše, Dejan Škorjanc, Janko Skok","doi":"10.1016/j.anireprosci.2024.107650","DOIUrl":"10.1016/j.anireprosci.2024.107650","url":null,"abstract":"<p><p>In livestock breeding, offspring of a particular sex are often favoured. Various biological mechanisms influence the offspring sex ratio (OSR). It has been hypothesised that maternal body condition/nutritional status influence OSR. We analysed the relationship between the nutritional status of the cow and OSR. The analysed dataset contained 40,913 milk recordings of 39,192 cows from 3625 farms. Nutritional status was estimated based on the fat-to-protein (F:P) ratio and urea content in milk in the seven to fourteen days prior to conception. Somatic cell count (SCC) was also considered. Cows with > 150,000 SSC were analysed separately. When SSC was normal (≤150,000), high urea with high F:P resulted in a higher probability of male offspring (61 %), with OSR significantly deviating towards male (1.47). Conversely, a high urea level and low F:P resulted in a higher probability of female offspring (57 %), with OSR significantly in favour of females (0.79). At normal or low milk urea, the sex of the offspring did not change within F:P classes. The sex ratio did not deviate from 1:1 when F:P and urea were in the normal range. When SCC was high, the probability of particular sex did not change significantly in either combination of F:P and urea, but OSR showed a tendency toward males (OSR>1) when F:P and urea were low or normal. Our results confirmed the influence of maternal nutritional status on the offspring sex. Thus, adjusting nutrition in the pre-insemination period seems to be a way to influence the sex ratio of offspring in dairy cows.</p>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"272 ","pages":"107650"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142791014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effect of epidermal growth factor on cryopreservation of dromedary camel epididymal spermatozoa: Evidence from in vitro and in silico studies.","authors":"Sameh A Abdelnour, Wael A Khalil, Mahmoud A E Hassan, Ibrahim T El-Ratel, Mostafa A El-Harairy, Sherif M Dessouki, Kandil A A Attia","doi":"10.1016/j.anireprosci.2024.107662","DOIUrl":"10.1016/j.anireprosci.2024.107662","url":null,"abstract":"<p><p>Epidermal growth factor (EGF) plays a crucial role in maintaining male reproductive capacity in mammals, however, its protective effects on cryopreserved dromedary camel epididymal spermatozoa have not been thoroughly investigated. This study aims to investigate the potential protective role of EGF on cryopreserved camel epididymal spermatozoa, supported by evidence from a molecular docking study. We assessed sperm motility, kinematics parameters, oxidative stress, ultrastructural changes, apoptosis, and molecular docking markers in camel epididymal spermatozoa following cryopreservation. Camel epididymal spermatozoa (n = 30 pairs of testes) were collected from local slaughterhouses. The epididymal spermatozoa were diluted with a freezing medium (SHOTOR extender) supplemented with different concentrations of EGF; 0 (EGF0), 50 (EGF50), 100 (EGF100), 200 (EGF200), and 400 (EGF400) ng/mL in SHOTOR extender and cryopreserved using a standard protocol. All EGF groups showed significant improvements in sperm progressive motility, viability, and sperm membrane function after equilibration at 5 °C for 24 hours. Regarding frozen-thawed samples, sperm progressive motility and some kinematic parameters (DAP, VSL, VCL and AHL) were significantly higher in the EFG400 group compared to the other groups (P < 0.01). A significant increase in the percentage of live/acrosome-intact sperm was observed, accompanied by a significant decrease in malondialdehyde levels in all EGF groups (P < 0.05). Both the EGF200 and EGF400 groups showed significantly higher sperm viability and significantly lower percentages of apoptotic and necrotic sperm compared to the other groups (P < 0.05). EGF supplementation preserved the ultrastructural integrity and cryotolerance of epididymal camel spermatozoa. The docking analysis indicated that EGF exhibited higher binding affinity with apoptosis sperm markers, including caspase-3 and bcl-2-associated X (Bax) proteins, with binding energies of -502.0 and -621.0 kcal/mol, respectively. In conclusion, the addition of EGF to SHOTOR extender was found to have beneficial effects on sperm motility, kinematics parameters, sperm viability, acrosome integrity, sperm ultrastructural features, and reduced oxidative stress and apoptosis-like changes in cryopreserved epididymal camel spermatozoa.</p>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"272 ","pages":"107662"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142791016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Strategies to increase circulating progesterone in late metestrus and early diestrus in recipient dairy heifers and cows: A focus on induction of accessory corpus luteum formation.","authors":"Marcelo S El Azzi, João Paulo N Martins, José C de Souza","doi":"10.1016/j.anireprosci.2024.107652","DOIUrl":"10.1016/j.anireprosci.2024.107652","url":null,"abstract":"<p><p>The capacity of recipients to support transferred embryos is a challenge for dairy cattle production systems. This review explores methods for increasing P4 to improve early pregnancy establishment in dairy cattle. A key aspect of P4 supplementation during the critical first weeks of pregnancy is its correlation with successful embryo elongation. Moreover, P4 was correlated to an increased chance of embryo survival after maternal recognition of pregnancy. Internal releasing devices increase circulating P4 concentrations but may negatively impact fertility possibly due to unwanted health pathologies such as vaginitis and the still undefined mechanism of haste luteolysis. The accessory corpus luteum (CL) induction in recipient dairy cattle increases endogenous P4 output increasing circulating P4 concentrations. Two ovulatory inducers are commonly used: gonadotropin-releasing hormone (GnRH) and human chorionic gonadotropin (hCG). Despite their distinct structures, GnRH and hCG are equally capable of inducing ovulation in cattle. The P4 supplementation by an accessory CL formation was widely used in fixed-time artificial insemination breeding programs, but still not exhaustively studied in embryo transfer (ET) programs. The induction of an accessory CL in ET programs has yielded inconsistent results depending on parity, embryo type production, embryo stage of development, and type of hormone used. Although maternal physiological mechanisms support the idea of increasing P4 for higher fertility and better embryo development, the actual results in creating a high-P4 maternal environment are still uncertain.</p>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"272 ","pages":"107652"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142791019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnosing american bison pregnancy during its two last trimesters: Comparison between rectal palpation associated with ultrasonography and hormonal assays in serum or in feces.","authors":"Vincent Frisée, Goulven Rigaux, Patrice Dufour, Flore Brutinel, Philippe Bossaert, Sophie Egyptien, Olimpia Barbato, Frédéric Farnir, Caroline Le Goff, Stéfan Deleuze, Etienne Cavalier, Jérôme Ponthier","doi":"10.1016/j.anireprosci.2024.107660","DOIUrl":"10.1016/j.anireprosci.2024.107660","url":null,"abstract":"<p><p>The study aimed to propose sensitive, specific and easy pregnancy diagnosis for American Bison. Trans-rectal Palpation and Ultrasonography (TRPUS), concentration of Pregnancy Associated Glycoproteins (PAG), Progesterone (P4), Estrone (E1), Estrone-Sulfate (E1S) in serum and P4 and E1S in feces were compared. Immunoassay was used for PAG, whereas steroids were assayed using Liquid Chromatography coupled to Mass Spectrometry. Pregnancy and its period were retrospectively assessed after calving. Animals included were distributed as follows: 40 were non-pregnant, 27 were in their second and 15 in their third trimester of gestation. Fisher's test was used to determine sensitivity and specificity of TRPUS. Thresholds leading to best sensitivities and specificities for hormonal assays were determined using Receiver Operating Characteristic Curves. Although TRPUS requires contention, palpation skills, it was conclusive in 70.63 % of cases and good sensitivity (97.50 %) and decent specificity (83.33 %) were observed. Concentrations of PAG, P4, E1S and E1 in serum were higher in Pregnant animals (P), with thresholds giving decent specificity and sensitivity (lower sensitivity for E1 and E1S: 92.11 %; lower specificity for P4: 93.48 %). In feces, P4 and E1S were higher in P: pregnancy diagnosis using E1S assay in feces showed 100 % of sensitivity and specificity. In conclusion, assaying E1S in feces during the two last trimesters of American Bisons pregnancy is a sensitive and specific gestation diagnosis. Pregnancy diagnosis using TRPUS can be useful in ranches but requires contention and skill adaptation. Serum hormonal assays can help evaluating the pregnancy period but also require contention.</p>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"272 ","pages":"107660"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142783872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alpha-Lipoic acid supplementation during in vitro culture of abattoir- and OPU-derived bovine oocytes improves embryonic quality.","authors":"Mariana C Fabra, Anabella A Campagna, Nicolás Farnetano, Juan P Anchordoquy, Juan M Anchordoquy, Ana C Carranza-Martin, Cecilia C Furnus, Noelia Nikoloff","doi":"10.1016/j.anireprosci.2024.107667","DOIUrl":"10.1016/j.anireprosci.2024.107667","url":null,"abstract":"<p><p>The Ovum Pick-Up (OPU) technique offers a rapid path to genetic improvement. Embryonic genome activation (EGA) is crucial for successful embryonic development and occurs in two phases: minor EGA (MN_EGA) from 2-cell to 8-cell stages, and major EGA (MJ_EGA) from 8-cell to 16-cell stages. Supplementation with alpha-lipoic acid (ALA) during MN_EGA using abattoir derived-oocytes has been shown to enhance in vitro embryo production. Two experiments were carried out in the study at hand. Experiment I aimed to evaluate ALA effects on embryo development and quality during MJ_EGA using abattoir-derived oocytes. Oocytes were treated with either a Control in vitro culture (IVC) medium or 2.5 µM ALA during MJ_EGA. The ALA presented a trend to increase the blastocyst cell number on day 7 (P = 0.06), along with reducing ROS levels and increasing GSH levels (P ≤ 0.05). Experiment II aimed to verify whether the results from abattoir- derived oocytes could be extrapolated to OPU-derived oocytes. The treatments included a Control, 2.5 µM ALA during MN_EGA, and 2.5 µM ALA during MJ_EGA. The ALA supplementation during MJ_EGA improved the total blastocyst rate on day 8 (P = 0.04), with a trend towards increased blastocyst cell number on day 7 (P = 0.09). No improvements were observed in MN_EGA. Overall, ALA supplementation during MJ_EGA is more effective than during MN_EGA for improving the embryonic quality of OPU-derived oocytes.</p>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"272 ","pages":"107667"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142806045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of maternal heat stress at different stages of pregnancy on the reproductive performance and antral follicle count of the progeny of Holstein cows.","authors":"Eduardo Alves Lima, Laís Reis Carvalho, Raphael Evangelista Orlandi, Luiz Manoel Souza Simões, Miguel Pizzolante Bottino, Ana Paula Castro Santos, Fernando de Oliveira Scarpa, Jose Nelio de Sousa Sales","doi":"10.1016/j.anireprosci.2024.107665","DOIUrl":"10.1016/j.anireprosci.2024.107665","url":null,"abstract":"<p><p>The objective was to evaluate the effect of maternal exposure to heat stress conditions at different stages of pregnancy on the reproductive performance and antral follicle count of progeny. The study was conducted at five commercial dairy farms located in southeastern Brazil. Retrospective data (2002 and 2015) on reproductive indices of daughters of Holstein cows that were exposed to heat stress conditions in the first (n = 447), second (n = 729) or third (n = 746) trimester of pregnancy and daughters of cows not exposed to heat stress (n = 359) were analyzed. Antral follicle count (AFC) and ovarian size were determined in a subgroup of animals (n = 242) by transrectal ultrasound. Statistical analysis was performed using the SAS GLIMMIX procedure. The number of services after first calving was lower in daughters of cows not exposed to heat stress conditions than in those exposed during different trimesters of pregnancy (P = 0.03). The calving interval was longer for daughters of cows exposed to heat stress conditions in the second and third trimesters of pregnancy than for those not exposed to heat stress (P = 0.01). In addition, the probability of pregnancy at first AI, (P = 0.01) and the AFC (P = 0.001) were greater in daughters of cows not exposed to heat stress conditions than in those exposed during the different phases of the gestational period. These findings indicate that maternal heat stress in different stages of pregnancy negatively affect the future fertility and AFC of bovine offspring.</p>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"272 ","pages":"107665"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142817025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}