Animal Reproduction Science最新文献_第3页

Evaluation of sperm quality and male fertility: The use of molecular markers in boar sperm and seminal plasma 评估精子质量和男性生育能力：使用公猪精子和精浆中的分子标记。

IF 2.2 2区农林科学

Animal Reproduction Science Pub Date : 2024-10-01 DOI: 10.1016/j.anireprosci.2024.107545

{"title":"Evaluation of sperm quality and male fertility: The use of molecular markers in boar sperm and seminal plasma","authors":"","doi":"10.1016/j.anireprosci.2024.107545","DOIUrl":"10.1016/j.anireprosci.2024.107545","url":null,"abstract":"<div><div><span><span><span>In pig production, the optimization of </span>artificial insemination<span> (AI) efficiency significantly relies on the accurate assessment of semen quality and fertility of boars. Traditional methods such as conventional seminogram techniques, although long-standing, exhibit limited sensitivity in predicting boar fertility, warranting the exploration of novel molecular markers. This review synthesizes the current knowledge on the utilization of molecular markers for semen quality evaluation and </span></span>male fertility prediction in boars, providing an in-depth examination of molecular markers in this context. Specifically, the present work delves into the potential of OMICs technologies, encompassing </span>genetic<span><span> and genomic approaches, transcriptomics, </span>proteomics<span><span>, and metabolomics<span>. A diverse array of molecular markers, including genomic regions associated with sperm quality and male fertility, chromatin integrity, mitochondrial </span></span>DNA content<span>, mRNA and non-coding RNA signatures, as well as proteins and metabolites in sperm and seminal plasma, are identified as promising molecular markers for fertility prediction in boars. Furthermore, the need of validating biomarkers and their practical implementation in AI centres is here emphasized. Addressing these considerations and integrating molecular markers within the swine breeding field holds the potential to enhance reproductive management practices and optimize productivity in boar breeding programs. This integration can significantly improve overall efficiency within the pig breeding industry.</span></span></span></div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"269 ","pages":"Article 107545"},"PeriodicalIF":2.2,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141496895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic, transcriptomic and epigenomic analysis towards the understanding of porcine semen quality traits. Past, current and future trends 通过基因组、转录组和表观基因组分析了解猪精液质量特征。过去、现在和未来的趋势。

IF 2.2 2区农林科学

Animal Reproduction Science Pub Date : 2024-10-01 DOI: 10.1016/j.anireprosci.2024.107543

{"title":"Genomic, transcriptomic and epigenomic analysis towards the understanding of porcine semen quality traits. Past, current and future trends","authors":"","doi":"10.1016/j.anireprosci.2024.107543","DOIUrl":"10.1016/j.anireprosci.2024.107543","url":null,"abstract":"<div><div>The importance of boar reproductive traits, including semen quality, in the sustainability of pig production system is increasingly being acknowledged by academic and industrial sectors. Research is needed to understand the biology and genetic components underlying these traits so that they can be incorporated into selection schemes and managerial decisions. This article reviews our current understanding of genome biology and technologies for genome, transcriptome and epigenome analysis which now facilitate the identification of causal variants affecting phenotypes more than ever before. Genetic and transcriptomic analysis of candidate genes, Genome-Wide Association Studies, expression microarrays, RNA-Seq of coding and noncoding genes and epigenomic evaluations have been conducted to profile the molecular makeups of pig sperm. These studies have provided insightful information for a several semen-related parameters. Nonetheless, this research is still incipient. The spermatozoon harbors a reduced transcriptome and highly modified epigenome, and it is assumed to be transcriptionally silent for nuclear gene expression. For this reason, the extent to which the sperm’s RNA and epigenome recapitulate sperm biology and function is unclear. Hence, we anticipate that single-cell level analyses of the testicle and other male reproductive organs, which can reveal active transcription and epigenomic profiles in cells influencing sperm quality, will gain popularity and markedly advance our understanding of sperm-related traits. Future research will delve deeper into sperm fertility, boar resilience to environmental changes or harsh conditions, especially in the context of global warming, and also in transgenerational inheritance and how the environment influences the sperm transcriptome and epigenome.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"269 ","pages":"Article 107543"},"PeriodicalIF":2.2,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141562456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Contaminant toxicity of concern for boars and semen used in assisted reproduction programs 辅助生殖计划中使用的公猪和精液的污染物毒性问题

IF 2.2 2区农林科学

Animal Reproduction Science Pub Date : 2024-10-01 DOI: 10.1016/j.anireprosci.2024.107519

{"title":"Contaminant toxicity of concern for boars and semen used in assisted reproduction programs","authors":"","doi":"10.1016/j.anireprosci.2024.107519","DOIUrl":"10.1016/j.anireprosci.2024.107519","url":null,"abstract":"<div><div><span>The commercial swine industry utilizes artificial insemination<span><span> (AI) in their breeding programs. With this assisted reproductive technology, the process starts by obtaining fresh ejaculates from desirable boars who are housed in a dedicated facility (i.e., stud) that also contains a clean-room laboratory where semen quality is assessed and then ejaculates processed into AI doses. In concert with AI adoption, disruptions in sow herd </span>reproductive performance have been traced back to contributions made from the boar stud. Through field investigations and research, several extrinsic contaminants have been identified that impact semen quality either at the boar or AI-dose level. These contaminants can be categorized as either biological or chemical in origin, eliciting reprotoxic outcomes at the boar level and/or spermatotoxicity at the AI-dose level. Biological contaminants include multiple genera of primarily opportunistic microbes (i.e., bacteria, fungi), along with their </span></span>secondary metabolites<span><span> (e.g., endotoxins<span><span>, exotoxins, mycotoxins). Chemical contaminants appear to originate from products used at the stud, and include cleaning agent/disinfectant residues, leachates from gloves and plastics, </span>semen extender impurities, purified and drinking water impurities, and pesticides (i.e., </span></span>biocides<span>, fungicides, herbicides, insecticides, wood preservatives). In conclusion, contaminants are a real and constant threat to the health and productivity of a stud, and have caused significant reproductive and economic losses in the swine industry. The knowledge gained in recognizing the types and sources of contaminants provides a solid foundation for the development and implementation of pro-active strategies that mitigate risk to the industry.</span></span></div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"269 ","pages":"Article 107519"},"PeriodicalIF":2.2,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141413979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Editorial: 10th International Conference on Boar Semen Preservation 社论：第 10 届国际野猪精液保存会议。

IF 2.2 2区农林科学

Animal Reproduction Science Pub Date : 2024-10-01 DOI: 10.1016/j.anireprosci.2024.107575

Marc Yeste

引用次数: 0

Modern feeding strategies for breeding boars 种公猪的现代饲养策略

IF 2.2 2区农林科学

Animal Reproduction Science Pub Date : 2024-10-01 DOI: 10.1016/j.anireprosci.2024.107497

{"title":"Modern feeding strategies for breeding boars","authors":"","doi":"10.1016/j.anireprosci.2024.107497","DOIUrl":"10.1016/j.anireprosci.2024.107497","url":null,"abstract":"<div><div><span>Feeding of breeding boars in artificial insemination<span> (AI) centers is critical to maintaining and improving breeding quality and performance in agriculture<span>. Modern feeding strategies for AI boars are aiming towards maximizing their lifetime semen dose output. Given the high growth potential of modern swine genetics, AI boars should be controlled in their daily gain to reduce stress factors for the locomotion system, final </span></span></span>body weights<span><span>, and improve their survivability but also the ease of handling boars. The feeding program should be designed in such a way that young boars (up to 7 months of age) are limited to a daily gain of 400–600 g. Mature boars should be fed towards a body condition<span> score of ‘2’. Aside from energy intake, protein sources should provide 0.62% SID lysine. As far as minerals and vitamins, special attention should be given to Calcium and Phosphorus as they play a crucial role in bone mineralization. A standardized total tract digestible Calcium-Phosphorus ratio between 1.75:1 and 1.82:1 seems to be most favorable. While certain nutritional requirements are needed to enable the production of ejaculates eligible for AI, considerations should be given to the mitigation of </span></span>risk factors<span> like mycotoxins<span>, herbicides, and pesticides. Most feed additives and supplements lack consistency in their effect across studies evaluated in this review.</span></span></span></div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"269 ","pages":"Article 107497"},"PeriodicalIF":2.2,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140921078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Swine fertility in a changing climate 不断变化的气候中的猪繁殖力。

IF 2.2 2区农林科学

Animal Reproduction Science Pub Date : 2024-10-01 DOI: 10.1016/j.anireprosci.2024.107537

{"title":"Swine fertility in a changing climate","authors":"","doi":"10.1016/j.anireprosci.2024.107537","DOIUrl":"10.1016/j.anireprosci.2024.107537","url":null,"abstract":"<div><div>Climate change has been linked to increasing temperatures and weather extremes. Certain regions around the world become more susceptible to environmental hazards that limit pig production and reproductive fertility. Environmental measures that link to pig fertility are needed to assess change, risk and develop solutions. Sub-populations of pigs display lower fertility in summer and are susceptible to heat stress. In the context of a warming climate, elevated temperatures and number of heat stress days increase body temperature and change the physiology, behavior, feed intake, and stress response of the pig. These changes could alter follicle development, oocyte quality, estrus expression, conception and litter size. In boars, sperm quality and production are reduced in response to summer heat stress. Nevertheless, while temperature increases have occurred over the years in some warmer locations, other regions have not shown those changes. Perhaps this involves the measures used for heat stress assessment or that climate is buffered in more temperate areas. Reductions in pig fertility are not always evident, and depend upon climate, year, genotype and management. This could also involve selection, as females more susceptible to heat stress and fertility failure, are subsequently culled. In the years from 1999 to 2020 when increases in global temperature from baseline occurred, measures of female fertility improved for farrowing rate and litter size. Progressive reduction in fertility may not be apparent in all geo-locations, but as temperatures increases become more widespread, these changes are likely to become more obvious and detectable.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"269 ","pages":"Article 107537"},"PeriodicalIF":2.2,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141449362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrating metabolomics into reproduction: Sperm metabolism and fertility enhancement in pigs 将代谢组学融入繁殖：猪的精子代谢和繁殖力提高。

IF 2.2 2区农林科学

Animal Reproduction Science Pub Date : 2024-10-01 DOI: 10.1016/j.anireprosci.2024.107539

{"title":"Integrating metabolomics into reproduction: Sperm metabolism and fertility enhancement in pigs","authors":"","doi":"10.1016/j.anireprosci.2024.107539","DOIUrl":"10.1016/j.anireprosci.2024.107539","url":null,"abstract":"<div><div><span><span><span>The last decades of research have revealed that many other factors besides gamete genomes are able to determine the reproductive outcomes. Indeed, paternal factors have been observed to be capable of modulating multiple crucial features of the reproductive process, such as </span>sperm physiology<span>, the maternal environment and, even, the offspring health. These recent advances have been encompassed with the emergence of OMICS technologies, as they comprehensively characterise the molecular composition of biological systems. The present narrative review aimed to take a closer look at the potential of these technologies in the field of reproductive biology. This literature revision shows that most studies up to date have followed a non-targeted approach to screen mammalian seminal plasma (SP) and sperm metabolite composition through different </span></span>metabolome platforms. These studies have proposed metabolites of multiple natures as potential </span><em>in vivo</em><span><span><span><span> fertility biomarkers. Yet, targeted approaches can be used to answer specific biological question, and their power is exemplified herein. For instance, metabolomic studies have uncovered not only that glycolysis is the main ATP energy source of </span>pig sperm, but also that sperm metabolism can trigger DNA damage, hence compromise </span>embryo development. In conclusion, this review shows the potential of both non-targeted and targeted metabolomics for the discovery of cell pathways that govern the reproductive process. Understanding these systems could help make progress in different areas, including </span>livestock efficient breeding, the improvement of artificial reproductive technologies, and the development of biomarkers for infertility detection.</span></div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"269 ","pages":"Article 107539"},"PeriodicalIF":2.2,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141454697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reproductive physiology of the boar: What defines the potential fertility of an ejaculate? 公猪的生殖生理：是什么决定了射精的潜在生育能力？

IF 2.2 2区农林科学

Animal Reproduction Science Pub Date : 2024-10-01 DOI: 10.1016/j.anireprosci.2024.107476

{"title":"Reproductive physiology of the boar: What defines the potential fertility of an ejaculate?","authors":"","doi":"10.1016/j.anireprosci.2024.107476","DOIUrl":"10.1016/j.anireprosci.2024.107476","url":null,"abstract":"<div><div>Despite decades of research and handling of semen for use in artificial insemination (AI) and other assisted reproductive technologies, 5–10% of selected boar sires are still considered sub-fertile, escaping current assessment methods for sperm quality and resilience to preservation. As end-product, the ejaculate (emitted spermatozoa sequentially exposed to the composite seminal plasma, the SP) ought to define the homeostasis of the testes, the epididymis, and the accessory sexual glands. Yet, linking findings in the ejaculate to sperm production biology and fertility is suboptimal. The present essay critically reviews how the ejaculate of a fertile boar can help us to diagnose both reproductive health and resilience to semen handling, focusing on methods -available and under development- to identify suitable biomarkers for cryotolerance and fertility. Bulk SP, semen proteins and microRNAs (miRNAs) have, albeit linked to sperm function and fertility after AI, failed to enhance reproductive outcomes at commercial level, perhaps for just being components of a complex functional pathway. Hence, focus is now on the interaction sperm-SP, comparing <em>in vivo</em> with <em>ex vivo</em>, and regarding nano-sized lipid bilayer seminal extracellular vesicles (sEVs) as priority. sEVs transport fragile molecules (lipids, proteins, nucleic acids) which, shielded from degradation, mediate cell-to-cell communication with spermatozoa and the female internal genital tract. Such interaction modulates essential reproductive processes, from sperm homeostasis to immunological female tolerance. sEVs can be harvested, characterized, stored, and manipulated, e.g. can be used for andrological diagnosis, selection of breeders, and alternatively be used as additives to improve cryosurvival and fertility.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"269 ","pages":"Article 107476"},"PeriodicalIF":2.2,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140764465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selection and direct biomarkers of reproductive capacity of breeding boars 种公猪繁殖能力的选择和直接生物标记。

IF 2.2 2区农林科学

Animal Reproduction Science Pub Date : 2024-10-01 DOI: 10.1016/j.anireprosci.2024.107490

{"title":"Selection and direct biomarkers of reproductive capacity of breeding boars","authors":"","doi":"10.1016/j.anireprosci.2024.107490","DOIUrl":"10.1016/j.anireprosci.2024.107490","url":null,"abstract":"<div><div><span><span><span>Efficient management of pig reproduction is paramount for the sustainability and productivity of the global pork industry. Modern </span>artificial insemination (AI) breeding programs have greatly benefited from the integration of advanced selection methods and biomarkers to enhance the </span>reproductive performance of boars. While traditional selection methods have relied soley on boar phenotype, such as growth rate and conformation, modern pig breeding has shifted more and more toward molecular and </span>genetic<span><span><span> tools, which are still complemented by phenotypic traits. These methods encompass genomics, transcriptomics, and </span>proteomics. Biomarkers serve as critical indicators of boar reproductive capacity. They can help to identify individuals with superior fertility and aid in the early identification of potential fertility issues, allowing for proactive management strategies. This review summarizes current knowledge of various biomarkers associated with semen quality, sperm function, and overall </span>reproductive fitness<span> in boars. Furthermore, we explore advanced technologies and their potential applications in uncovering novel selection methods and biomarkers for predicting boar fertility. A comprehensive understanding of selection criteria and biomarkers governing boar reproductive capacity is essential for developing effective breeding programs to enhance swine reproductive performance.</span></span></div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"269 ","pages":"Article 107490"},"PeriodicalIF":2.2,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140910846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of the utility of PCR-RFLP as a rapid alternative to DNA sequencing for interrogation of the genetic sex of Mugil cephalus 研究用 PCR-RFLP 快速替代 DNA 测序来检测鲻鱼的遗传性别。

IF 2.2 2区农林科学

Animal Reproduction Science Pub Date : 2024-10-01 DOI: 10.1016/j.anireprosci.2024.107614

Karl B. Andree , Barbara Loi , Dario Vallainc , Danilo Concu , Neil Duncan , Stefano Carboni

{"title":"Investigation of the utility of PCR-RFLP as a rapid alternative to DNA sequencing for interrogation of the genetic sex of Mugil cephalus","authors":"Karl B. Andree , Barbara Loi , Dario Vallainc , Danilo Concu , Neil Duncan , Stefano Carboni","doi":"10.1016/j.anireprosci.2024.107614","DOIUrl":"10.1016/j.anireprosci.2024.107614","url":null,"abstract":"<div><div><em>Mugil cephalus</em> is a species of considerable interest for aquaculture. As a species that is not sexually dimorphic, when building a brood stock with a balanced sex ratio there is difficulty for identification of sex until the animals are quite mature. When mono-sex populations of this species is desired, as in the case of production of females for “bottarga”, considerable resource expenditure could be saved if early sorting of sexes were possible to enable selection of a single sex. A recently described sex-associated loci within the follicle stimulating hormone receptor gene (<em>fshr</em>) was identified using genomic DNA sequencing and shown to contain some non-synonymous mutations wherein the females have a tendency to be homozygous and the males heterozygous. The loci identification method is time-consuming and somewhat expensive. We propose that the method described for the identification of the genetic sex of <em>Mugil cephalus,</em> prior to sexual maturation should be rapid and not require DNA sequencing. In this work, we demonstrate the utilization of one of these <em>fshr</em> mutations within this genetic marker in a PCR-RFLP assay. Using this new method, the loci in question shows 77.8 % partitioning with males and 88.9 % partition with females, as referenced to phenotypic sex characterized by histology, thus confirming the partitioning of the genetic marker as seen previously using DNA sequencing. Future applications of this relatively rapid and inexpensive method could contribute to the production of mono-sex farmed stock and open new opportunities for more efficient broodstock management practices in the species.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"270 ","pages":"Article 107614"},"PeriodicalIF":2.2,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142374907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0