Animal Reproduction Science最新文献_第3页

The phenotype, sex ratio and gonadal development in triploid hybrids of rainbow trout (Oncorhynchus mykiss) ♀ and brook trout (Salvelinus fontinalis) ♂. 虹鳟（Oncorhynchus mykiss）♀和溪鳟（Salvelinus fontinalis）♂三倍体杂种的表型、性别比和性腺发育。

IF 2.2 2区农林科学

Animal Reproduction Science Pub Date : 2025-01-01 Epub Date: 2024-11-30 DOI: 10.1016/j.anireprosci.2024.107659

Marcin Kuciński, Paulina Trzeciak, Ziemowit Pirtań, Wojciech Jóźwiak, Konrad Ocalewicz

{"title":"The phenotype, sex ratio and gonadal development in triploid hybrids of rainbow trout (Oncorhynchus mykiss) ♀ and brook trout (Salvelinus fontinalis) ♂.","authors":"Marcin Kuciński, Paulina Trzeciak, Ziemowit Pirtań, Wojciech Jóźwiak, Konrad Ocalewicz","doi":"10.1016/j.anireprosci.2024.107659","DOIUrl":"10.1016/j.anireprosci.2024.107659","url":null,"abstract":"<p><p>The phenotype, genetic sex ratio and gonadal development characteristics were evaluated in randomly selected juvenile (15 months old), sub-adult (22 months old) and adult (30 months old) triploid hybrids of rainbow trout (Oncorhynchus mykiss) and brook trout (Salvelinus fontinalis). The examined fish originated from the family produced by application High Hydrostatic Pressure (HHP) shock (9500 psi for 5 minutes, applied 35 minutes post-fertilization at 10°C) to eggs stripped from five rainbow trout females and fertilized with sperm of two brook trout males. After hatching, all fish were reared under the same conditions for three years. The ploidy level and hybrid status of the fish were confirmed through cytogenetic analysis and DNA genotyping. Three distinct phenotypes; trout-like (Tr-l) phenotype, salmon-like (Sa-l) phenotype and intermediate (Tr/Sa-l) phenotype, with the following frequencies: f= 50.8 %, f= 31.7 % and f= 17.5 %, respectively, were observed among the sampled specimens. Genetic males exclusively detected among individuals with Tr-l phenotype developed macroscopically visible and histologically functional testes in their second year of life, representing the lowest value for the aquaculture production. A comparable proportion of males and females was recorded among individuals with Sa-l and Tr/Sa-l phenotypes. Males with these phenotypes developed macroscopically distinguishable and histologically functional testes in their third year of life. In the genetic males, underdeveloped intersex or completely reduced gonads were recorded. This study revealed that individuals with Sa-l phenotype represent the greatest value for commercial production because of their unique appearance and complete sterility (females) or delayed maturation observed only after reaching the market size (males).</p>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"272 ","pages":"107659"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142779461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of vibration duration on the quality of boar semen doses packed in bags or tubes. 振动时间对袋装或管装公猪精液质量的影响。

IF 2.2 2区农林科学

Animal Reproduction Science Pub Date : 2025-01-01 Epub Date: 2024-11-29 DOI: 10.1016/j.anireprosci.2024.107658

Gabriel A Bona, Rodrigo D Rech, Leonardo A Leal, Lucas M Wolf, Thaís S Christ, Martin Schulze, Fernando P Bortolozzo, Rafael R Ulguim, Ana Paula G Mellagi

{"title":"Influence of vibration duration on the quality of boar semen doses packed in bags or tubes.","authors":"Gabriel A Bona, Rodrigo D Rech, Leonardo A Leal, Lucas M Wolf, Thaís S Christ, Martin Schulze, Fernando P Bortolozzo, Rafael R Ulguim, Ana Paula G Mellagi","doi":"10.1016/j.anireprosci.2024.107658","DOIUrl":"10.1016/j.anireprosci.2024.107658","url":null,"abstract":"<p><p>As previous data showing the negative impact of semen agitation during transport on sperm quality was obtained only in tube-type packages, this study aimed to compare two packages for boar semen dose (bag or tube) submitted to different agitation times. Ejaculates from thirty different boars were diluted in BTS extender, packed in bags or tubes in split sample, and submitted to agitation for 0, 6, or 12 h at 100 rpm at 17 ºC. Semen doses were stored for up to 168 h and evaluated for quality traits. Analyses were conducted as repeated measures, including package type, agitation time, storage time (or incubation for TRT) and their interactions. A longer agitation time resulted in reduced sperm motility, increased pH, decreased apoptotic-like changes cells percentage, reduced mitochondrial activity, increased ROS, and decreased sperm motility after TRT (P < 0.05). Tube packages exhibited higher sperm motility during storage and after TRT, a higher proportion of cells with no apoptotic-like changes and cells with high mitochondrial activity than bags (P < 0.01). The significant interaction between storage time and package type (P < 0.01) showed that tubes had higher pH levels after 72 h, consistently increasing up to 168 h than bags. The integrity was significantly reduced in bags compared to tubes after 12 h of agitation at 120 h of storage (P < 0.01). Acrosome membrane integrity decreased with increased agitation time, which was particularly pronounced in bags compared to tubes (P < 0.05). Overall, tube packaging offered superior protection, particularly under prolonged agitation conditions.</p>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"272 ","pages":"107658"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142783875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sperm fitness assessment in poultry: Brief review of in vitro methods. 家禽精子适应性评估：体外方法综述。

IF 2.2 2区农林科学

Animal Reproduction Science Pub Date : 2025-01-01 Epub Date: 2024-12-06 DOI: 10.1016/j.anireprosci.2024.107666

Denise Calisto Bongalhardo

{"title":"Sperm fitness assessment in poultry: Brief review of in vitro methods.","authors":"Denise Calisto Bongalhardo","doi":"10.1016/j.anireprosci.2024.107666","DOIUrl":"10.1016/j.anireprosci.2024.107666","url":null,"abstract":"<p><p>In the poultry industry, every chick or poult produced has high economic value; therefore, selection of male breeders capable of transmitting desirable traits to their offspring has an important role in fertility and hatching success. Following either natural mating or artificial insemination, sperm must go through a sequence of steps to fertilize the egg: 1. Go from the site of deposition (vagina) to the Sperm Storage Tubules (SST); 2. Enter and stay inside the SST; 3. Reacquire motility; and 4. Bind to and penetrate the egg. To perform these tasks successfully, sperm must be alive, motile, and have structural integrity (membrane, glycocalyx, acrosome, and DNA). To evaluate sperm fitness, several in vitro tests can be performed; however, it is often necessary to combine several assays to have a more reliable fertility estimation, as each procedure evaluates specific and distinct sperm features. In this article, we briefly review in vitro tests that can be used to evaluate poultry sperm, associating them with each step for fertilization. The sperm-egg interaction assay using the inner perivitelline layer of chicken eggs is highlighted, since it is a single test that evaluates multiple sperm characteristics and is highly correlated with fertility.</p>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"272 ","pages":"107666"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142806048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IF 2.2 2区农林科学

Animal Reproduction Science Pub Date : 2025-01-01 Epub Date: 2024-11-29 DOI: 10.1016/j.anireprosci.2024.107657

B Hensel, M Schulze

引用次数: 0

Supplementation of Epimedium polysaccharide (EPS) improves goat semen characteristics following cryopreservation. 添加淫羊藿多糖（EPS）可改善山羊精液冷冻保存后的性状。

IF 2.2 2区农林科学

Animal Reproduction Science Pub Date : 2025-01-01 Epub Date: 2024-11-29 DOI: 10.1016/j.anireprosci.2024.107654

Yu Li, Hui Wang, Zhangtao Hu, Guoyu Zhang, Fei Wen, Ming Xian, Songmao Guo, Guangzhi Zhang, Xing Zhang, Jianhong Hu

{"title":"Supplementation of Epimedium polysaccharide (EPS) improves goat semen characteristics following cryopreservation.","authors":"Yu Li, Hui Wang, Zhangtao Hu, Guoyu Zhang, Fei Wen, Ming Xian, Songmao Guo, Guangzhi Zhang, Xing Zhang, Jianhong Hu","doi":"10.1016/j.anireprosci.2024.107654","DOIUrl":"10.1016/j.anireprosci.2024.107654","url":null,"abstract":"<p><p>Cryopreservation facilitates long-term semen storage and enables the exchange of genetic material among elite livestock over extensive distances. A decrease in sperm quality is an unavoidable outcome of the cryopreservation process. Prior research has established that incorporating cryoprotectants into the diluent can mitigate freeze-induced damage and enhance sperm quality. This study aims to assess the impact of Epimedium polysaccharide (EPS) on the cryopreservation of goat semen. Samples were obtained from six healthy goats following an initial examination. One portion of the semen was diluted with a base solution containing EPS for treatment purposes, whereas another was diluted without EPS, serving as the control. Results indicated that varying concentrations (1, 2, 3, 4, 5 mg/mL) of EPS in the diluent enhanced both physiological characteristics and antioxidant enzyme activities in cryopreserved goat sperm. Further analysis showed that the 3 mg/mL EPS concentration significantly improved sperm motility (52.10 %), plasma membrane integrity (57.01 %), tail plasma membrane integrity (52.37 %), acrosome integrity rate (52.45 %), and antioxidant enzyme activities relative to other groups (P < 0.05). Additionally, the inclusion of 3 mg/mL EPS substantially improved the sperm's fertilization capability. In conclusion, our experiments confirm that EPS supplementation significantly enhances sperm quality post-freezing, with 3 mg/mL identified as the optimal concentration.</p>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"272 ","pages":"107654"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142791021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NPC2, a seminal plasma protein with membrane cholesterol-binding ability, influences the cryotolerance and functionality of spermatozoa from Chino Santandereano bulls. NPC2是一种具有膜胆固醇结合能力的精浆蛋白，影响中国公牛精子的低温耐受性和功能。

IF 2.2 2区农林科学

Animal Reproduction Science Pub Date : 2024-12-30 DOI: 10.1016/j.anireprosci.2024.107758

Norberto Villa-Duque, Juan J Agudelo-Flórez, Ricci Terraza-Martinez, Elkin Romero-Cárdenas, Germán Gómez, Julian Valencia

{"title":"NPC2, a seminal plasma protein with membrane cholesterol-binding ability, influences the cryotolerance and functionality of spermatozoa from Chino Santandereano bulls.","authors":"Norberto Villa-Duque, Juan J Agudelo-Flórez, Ricci Terraza-Martinez, Elkin Romero-Cárdenas, Germán Gómez, Julian Valencia","doi":"10.1016/j.anireprosci.2024.107758","DOIUrl":"https://doi.org/10.1016/j.anireprosci.2024.107758","url":null,"abstract":"<p><p>Despite Latin America's rich biodiversity, active genetic material conservation programs are scarce. This study investigates potential freezability markers in both sperm and seminal plasma (SP) in Chino Santandereano, a Colombian Creole breed. Thirty ejaculates from ten Chino Santandereano bulls were cryopreserved and subsequently classified as of good (GFE) or poor (PFE) freezability according to their post-thaw total sperm motility (TMOT) and plasma membrane integrity (PMI). Sperm cholesterol and NPC2 protein levels in SP were measured prior to cryopreservation using colorimetric detection, densitometric quantification, and Western blot. Sperm functional competence (SFC), a functional test, was evaluated before and after cryopreservation. Correlation and t-student tests, a binomial regression model and two multiple linear regression models were utilized to evaluate the predictive power of NPC2 protein and sperm related variables. Two bands (14 kDa and 15 kDa) for NPC2 protein were identificated. NPC2 14 kDa is associated with a significant decrease in freezability (P < 0.01). The TMOT in fresh semen highly significant (P < 0.01) for sperm freezability and the interaction between NPC2 14 kDa and cholesterol content in sperm was significant (P < 0.01). Correlation between SFC and TMOT and PMI post-thaw (P < 0.01), along with the significantly different values between PFE and GFE (P < 0.05) were observed. We can conclude that NPC2 protein as a marker for sperm freezability of Chino Santandereano bull, and this depends on sperm content of cholesterol. In addition, our findings support the utility SFC combined tests in providing a precise assessment of sperm freezability.</p>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"274 ","pages":"107758"},"PeriodicalIF":2.2,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142969418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New approaches in bovine spermatozoa evaluation and their relationship with male fertility 牛精子评价及其与雄性生育能力关系的新途径

IF 2.2 2区农林科学

Animal Reproduction Science Pub Date : 2024-11-26 DOI: 10.1016/j.anireprosci.2024.107656

Mayra Elena Ortiz D'Ávila Assumpção , Thais Rose dos Santos Hamilton

{"title":"New approaches in bovine spermatozoa evaluation and their relationship with male fertility","authors":"Mayra Elena Ortiz D'Ávila Assumpção , Thais Rose dos Santos Hamilton","doi":"10.1016/j.anireprosci.2024.107656","DOIUrl":"10.1016/j.anireprosci.2024.107656","url":null,"abstract":"<div><div>Male fertility potential depends on physical, endocrine, and genetic factors responsible for producing functional male gametes. Although the main function of the male gamete, the spermatozoon, is to deliver its genetic material to the oocyte, this premise has been modified over the past few years. It is believed that the spermatozoon provides essential factors for fertilization and pre-implantation embryo development. A viable/healthy spermatozoon has functional subcellular compartments (nucleus, acrosome, midpiece, and flagellum) due to the actions of proteins, transcripts, and epigenetic marks in the organelles present in them that have important roles in reproductive biology. Male fertility potential reflects viable spermatozoa with proper function. Therefore, new approaches to functional sperm analysis are essential. Additionally, intrinsic factors and sperm molecules constitute potential biomarkers of viable spermatozoa and male fertility. Among these factors are proteins, the genome, and coding and non-coding RNAs, such as microRNAs, that act during fertilization and early embryo development. Research has been seeking increasingly efficient tools to predict fertility and functional studies of these molecules through gene and protein expression. Thus, analytical tools are essential to identify and classify viable and functional spermatozoa, to evaluate assisted reproductive male potential.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"272 ","pages":"Article 107656"},"PeriodicalIF":2.2,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142743140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulatory T cells in bovine fertility: Current understanding and future prospects 调节性T细胞在牛生育中的作用：目前的认识和未来的展望

IF 2.2 2区农林科学

Animal Reproduction Science Pub Date : 2024-11-26 DOI: 10.1016/j.anireprosci.2024.107655

Arif Pandit , Syed M. Shah , Riaz A. Shah , Sabia Qureshi , R.S. Sethi , Faheem Bhat , Abrar Malik , Oveas Parray , Hilal Yaqoob , Masood Saleem

{"title":"Regulatory T cells in bovine fertility: Current understanding and future prospects","authors":"Arif Pandit , Syed M. Shah , Riaz A. Shah , Sabia Qureshi , R.S. Sethi , Faheem Bhat , Abrar Malik , Oveas Parray , Hilal Yaqoob , Masood Saleem","doi":"10.1016/j.anireprosci.2024.107655","DOIUrl":"10.1016/j.anireprosci.2024.107655","url":null,"abstract":"<div><div>Regulatory T cells (Tregs) have emerged as crucial players in maintaining maternal-fetal tolerance and promoting successful pregnancy outcomes. This review examines the importance of these cells in pregnancy, drawing on human and animal-based studies, with a focus on their role in bovine fertility. Tregs employ various mechanisms to mediate maternal-fetal tolerance, including regulation of effector T-cell responses, interactions with innate immune cells in the uterine microenvironment, and modulation of trophoblast function. In humans, Treg dynamics during normal pregnancy and alterations in pregnancy complications provide compelling evidence for their involvement in maintaining fetal-maternal harmony. Animal models, particularly mouse studies, have further elucidated the importance of Tregs in preventing fetal rejection and promoting successful pregnancy outcomes. The review also explores the characterization of bovine Tregs, highlighting their similarities and unique features compared to human and rodent counterparts. Recent studies have indicated the presence and potential significance of Tregs in the bovine uterine environment during early pregnancy. Translational applications of Treg research in livestock fertility are discussed, with a focus on immunomodulatory strategies for enhancing Treg function, such as antigen-specific tolerance induction, pharmacological targeting of Treg pathways, and cell-based therapies using autologous or allogeneic Tregs. The review concludes by emphasizing the potential impact of Treg-based strategies on the livestock industry and the broader implications for human reproductive health. Future research directions are outlined, underscoring the need for further investigations into the role of Tregs in bovine reproductive tissues and their relationship with fertility outcomes.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"272 ","pages":"Article 107655"},"PeriodicalIF":2.2,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142743141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Niosomal nanocarriers loaded with artemisinin has potential to enhance the functional characteristics of cryo-preserved equine spermatozoa 负载青蒿素的纳米载体有望增强低温保存的马精子的功能特性

IF 2.2 2区农林科学

Animal Reproduction Science Pub Date : 2024-11-26 DOI: 10.1016/j.anireprosci.2024.107653

Azam Abbasi , Houshang Nouri , Firooz Samadi

{"title":"Niosomal nanocarriers loaded with artemisinin has potential to enhance the functional characteristics of cryo-preserved equine spermatozoa","authors":"Azam Abbasi , Houshang Nouri , Firooz Samadi","doi":"10.1016/j.anireprosci.2024.107653","DOIUrl":"10.1016/j.anireprosci.2024.107653","url":null,"abstract":"<div><div>Cryopreservation is a crucial method for the preservation of genetic materials. Nevertheless, this technique can have adverse effects due to cryo-oxidative damage. The primary objective of this study was to investigate the cryo-protective effects of niosomal nanocarriers loaded with artemisinin (NN-Art) on the functional attributes of equine spermatozoa. The parameters examined included sperm motility and kinematics through computer-assisted sperm analysis (CASA), plasma membrane integrity via the hypoosmotic swelling test, viability using the eosin-nigrosin staining technique, morphology with Hancock's solution, and lipid peroxidation through the thiobarbituric acid reactive substances assay. Additionally, flow cytometry was applied to assess mitochondrial membrane potential, reactive oxygen species (ROS) levels, sperm chromatin structure, and apoptotic-like changes. The CASA indicated a greater enhancement in the total and progressive motility and as well straight line velocity following the addition of NN-Art into the cryo-diluent medium compared with the conventional form of artemisinin and control (P < 0.05). Moreover, the incorporation of cryo-diluent medium with NN-Art demonstrated a greater efficiency in the improvement of viability, proportion of spermatozoa abnormality, plasma membrane functionality, and lipid peroxidation (P < 0.05). The flow cytometry assessment demonstrated a significant enhancement in mitochondrial potential activity, a reduction in the level of ROS, and an increase in the proportion of live spermatozoa along with a decrease in late apoptotic stallion post-thawed spermatozoa treated with both Art and specifically NN-Art. The results of this study suggest that NN-Art exhibit enhanced antioxidant capabilities, which are crucial for improving the functional quality of equine spermatozoa by mitigating cryo-oxidative stress.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"272 ","pages":"Article 107653"},"PeriodicalIF":2.2,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142721596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acute heat stress regulates estradiol synthesis in ovine ovarian granulosa cells through the SREBPs/MVK–LHR pathway 急性热应激通过SREBPs/ MVK-LHR途径调节羊卵巢颗粒细胞雌二醇合成

IF 2.2 2区农林科学

Animal Reproduction Science Pub Date : 2024-11-19 DOI: 10.1016/j.anireprosci.2024.107649

Yinxia Li , Fan Li , Jiaao Shu , Chunhua Meng , Jun Zhang , Jianli Zhang , Yong Qian , Huili Wang , Qiang Ding , Shaoxian Cao

{"title":"Acute heat stress regulates estradiol synthesis in ovine ovarian granulosa cells through the SREBPs/MVK–LHR pathway","authors":"Yinxia Li , Fan Li , Jiaao Shu , Chunhua Meng , Jun Zhang , Jianli Zhang , Yong Qian , Huili Wang , Qiang Ding , Shaoxian Cao","doi":"10.1016/j.anireprosci.2024.107649","DOIUrl":"10.1016/j.anireprosci.2024.107649","url":null,"abstract":"<div><div>The adverse effects of heat stress on reproductive performance of sheep are becoming increasingly severe. Previous research has revealed that heat stress decreases both cholesterol and estradiol content; however, regulation of estradiol by cholesterol and its regulatory mechanism under heat stress are unclear. Mevalonate kinase (MVK), a key cholesterol synthesis pathway enzyme, binds to the luteinizing hormone receptor (LHR; a key gene regulating hormone synthesis) mRNA. In this study, ovine ovarian granulosa cells (GCs) were used in an <em>in vitro</em> model. To elucidate the underlying molecular mechanism, immunofluorescence, quantitative reverse transcription polymerase chain reaction, western blotting, enzyme-linked immunosorbent assay, and an RNA electrophoretic mobility shift assay (REMSA) were used to investigate whether the decrease in cholesterol caused by acute heat stress resulted in a decrease in estradiol synthesis. Acute heat stress reduced the cholesterol content in ovine ovarian GCs, which transactivated the cholesterol synthesis pathway corresponding to the gene expression of sterol regulatory element-binding protein (SREBP-1A), SREBP-2, and MVK. Upregulated MVK increased the MVK–<em>LHR</em> mRNA complex, which caused <em>LHR</em> mRNA decay and downregulation, further leading to the downregulation of CYP19A1 and a decrease in estradiol. The cholesterol synthesis inhibitor, PF-429242, alleviated the decrease in estradiol synthesis caused by acute heat stress. Overall, acute heat stress caused a decrease in total cholesterol, which transactivated the expression of cholesterol synthesis genes, such as <em>SREBP-1A</em>, <em>SREBP2</em>, and <em>MVK</em>, increasing the MVK–LHR complex, downregulating <em>LHR</em> expression, and further decreasing estradiol.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"272 ","pages":"Article 107649"},"PeriodicalIF":2.2,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142743139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0