Animal Reproduction Science最新文献

{"title":"Comprehensive analysis of non-coding RNAs in the ovaries of high and low egg production hens","authors":"Jintang Luo ,&nbsp;Tiantian Sun ,&nbsp;Siyi Jiang ,&nbsp;Zhuliang Yang ,&nbsp;Cong Xiao ,&nbsp;Jixian Deng ,&nbsp;Biyan Zhou ,&nbsp;Xiurong Yang","doi":"10.1016/j.anireprosci.2025.107836","DOIUrl":"10.1016/j.anireprosci.2025.107836","url":null,"abstract":"<div><div>Egg production performance a critical economic trait in the poultry industry. The regulatory mechanisms underlying egg production performance mediated by non-coding RNAs remain to be characterized. To systematically investigate ovarian lncRNAs, circRNAs, and miRNAs associated with laying efficiency, we conducted comparative transcriptomic analyses using RNA sequencing (RNA-seq) of ovarian tissues from phenotypically divergent groups - high egg production (HEP) and low egg production (LEP) hens. In our study, we identified 675 lncRNAs, 140 circRNAs, and 10 miRNAs that were significantly differentially expressed (DE) between HEP and LEP. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that target genes of DE lncRNAs, DE miRNAs, and the source genes of DE circRNAs are involved in the MAPK signaling pathway, endocytosis, notch signaling pathway, among others. Furthermore, we identified five miRNA-mRNA interactions related to egg production including gga-miR-449c-3p, and five genes (<em>GLI</em>2, <em>TAC</em>1, <em>EML</em>6, <em>THOC</em>3, <em>MMP</em>9). These findings establish the first comprehensive ncRNA interactome driving ovarian efficiency, offering both biomarkers for breeding selection and mechanistic targets for reproductive enhancement.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"276 ","pages":"Article 107836"},"PeriodicalIF":2.2,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143816945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of freezing and thawing techniques on the in vitro quality of pellet-frozen ram semen","authors":"E.A. Spanner,&nbsp;J.P. Rickard,&nbsp;S.P. de Graaf","doi":"10.1016/j.anireprosci.2025.107822","DOIUrl":"10.1016/j.anireprosci.2025.107822","url":null,"abstract":"<div><div>The post-thaw quality of pellet-frozen ram semen was evaluated based on (i) sperm concentration, (ii) thawing diluent, and (iii) the number of pellets thawed simultaneously. Ejaculates from three Merino rams were collected, with four replicates per ram (n = 12 ejaculates) for each experiment. In Experiment 1, ejaculates were frozen at 200, 400, 600 or 800 × 10⁶ sperm/ mL. In Experiment 2, ejaculates were frozen at 600 × 10⁶ sperm/ mL and thawed in a dry test tube (control) or with 1 + 3 tris-citrate-fructose, tris-citrate-fructose+egg yolk, PBS+dye, PBS alone or IVF media (Emcare). In Experiment 3, groups of 1, 2 or 3 pellets were thawed in tris-citrate-fructose or a dry test tube (control). Post-thaw samples were tested for motility, acrosome and membrane integrity (FITC-PNA/PI) at 0, 3, and 6 h (all Experiments) and morphology at 0 h (Experiment 1). At 3 and 6 h post-thaw, motility was reduced at 200 × 10⁶ sperm/mL compared to other concentrations (P &lt; 0.05). Across all time points, samples frozen at 800 × 10⁶ sperm/mL (P &lt; 0.05) showed lower viability, while freezing at 200 × 10⁶ sperm/mL increased morphological abnormalities (P &lt; 0.05). Thawing pellets with tris-citrate-fructose media resulted in higher motility, acrosome and membrane integrity 0, 3 and 6 h post-thaw (<em>P</em> &gt; 0.05). Thawing one-pellet in tris-citrate-fructose improved motility and viability post-thaw (P &lt; 0.05) compared to multiple pellets, while the number of pellets thawed had no significant effect when dry-thawed (P &gt; 0.05). The results suggest that the optimal post-thaw quality is achieved when semen is frozen between 400 and 600 × 10⁶ sperm/mL and thawed in tris-citrate-fructose media, one pellet at a time.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"276 ","pages":"Article 107822"},"PeriodicalIF":2.2,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143830100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The surface proteome of rabbit spermatozoa","authors":"Patrícia Pinto-Pinho ,&nbsp;Francis Impens ,&nbsp;Sara Dufour ,&nbsp;Delphi Van Haver ,&nbsp;Rosário Pinto-Leite ,&nbsp;John Howl ,&nbsp;Margarida Fardilha ,&nbsp;Bruno Colaço","doi":"10.1016/j.anireprosci.2025.107834","DOIUrl":"10.1016/j.anireprosci.2025.107834","url":null,"abstract":"<div><div>Cell surface proteins, targeted by approximately 70 % of current pharmaceuticals, offer promising prospects for therapeutic and biotechnological advancements. The recent identification of cell surface proteins, differentially expressed by rabbit spermatozoa, could support technologies to enable sperm sex selection. However, a more detailed knowledge of the rabbit sperm plasma membrane proteome is crucial to such developments. Hence, the primary objective of this study was to conduct a shotgun proteomic (LC-MS/MS) analysis of New Zealand White rabbit spermatozoa protein lysates enriched in cell surface proteins isolated through biotinylation. This approach was designed to provide an overall characterization of this proteome and so determine an expanded list of protein candidates with potential for rabbit sperm sexing. The most promising targets were identified through functional annotation (UniProt and eggNOG-mapper v.2.1.9) and topology prediction (DeepTMHMM v.1.0.13). Additionally, a statistical overrepresentation test (PANTHER 18.0) and analysis of protein–protein interactions (STRING v.12.0) were conducted. Among the 859 detected proteins, 803 had Gene Ontology information, with 574 predicted as globular proteins, 152 as transmembrane proteins, and 133 possessing only a signal peptide. The combined data identified 107 proteins as potential cell surface targets, including three transmembrane proteins encoded by the X chromosome (ADGRG2, ATP6AP2, and VSIG1). Furthermore, two proteins (BCAP31 and PGRMC1), previously identified as putative rabbit X-targets, were recognized. This study enhances our comprehension of rabbit spermatozoa proteomics. Further validation of the utility of these five proteins to differentiate between X- and Y-sperm will determine their suitability for integration into sperm sexing technologies.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"276 ","pages":"Article 107834"},"PeriodicalIF":2.2,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143791639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of maternal dietary protein on livestock development, production and health","authors":"Shize Xiao ,&nbsp;Wenju Liu ,&nbsp;Shujun Zhang ,&nbsp;Martine Schroyen","doi":"10.1016/j.anireprosci.2025.107835","DOIUrl":"10.1016/j.anireprosci.2025.107835","url":null,"abstract":"<div><div>Maternal dietary protein plays a pivotal role in shaping offspring development, health, and productivity in economically important livestock species, including pigs, cattle, and sheep. Protein intake during gestation influences multiple physiological processes in the offspring, such as fetal growth, metabolic programming, muscle development, immune function, reproduction, and gut health. The specific effects of maternal protein intake vary depending on the species and the gestational period, as the demands for protein fluctuate throughout pregnancy to support fetal development and postnatal adaptation. This review systematically explores the effects of maternal protein nutrition on the offspring of different species and identifies the commonalities and differences observed in the studies. Studies indicate that maternal protein restriction can lead to lower birth weights, impaired muscle growth, altered metabolic programming, and compromised immune function in offspring, potentially affecting their long-term productivity. Conversely, excessive protein intake may also have adverse effects, such as immune dysregulation and metabolic imbalances. The impact of maternal protein levels extends beyond birth, influencing postnatal growth trajectories, reproductive performance, and gut microbiota composition. While considerable progress has been made in understanding these relationships, gaps remain in identifying the precise molecular mechanisms underlying these effects. Future research should focus on refining dietary recommendations tailored to different livestock species, investigating the role of gestation stage-specific protein requirements, and integrating multi-omics approaches to elucidate the long-term consequences of maternal protein intake. A deeper understanding of these mechanisms will contribute to optimizing feeding strategies, enhancing animal welfare, and improving the sustainability of livestock production systems.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"276 ","pages":"Article 107835"},"PeriodicalIF":2.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143785968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differences in microRNA within plasma extracellular vesicles at various time points during early pregnancy in sheep revealed by high-throughput sequencing data","authors":"Zhanmin Lv ,&nbsp;Zhenliang Zhang ,&nbsp;Yucheng Liu ,&nbsp;Jingjing Wang ,&nbsp;Weijun Liu ,&nbsp;Pengcheng Wan ,&nbsp;Jianhong Ni ,&nbsp;Chunxia Lu ,&nbsp;Changbin Liu","doi":"10.1016/j.anireprosci.2025.107833","DOIUrl":"10.1016/j.anireprosci.2025.107833","url":null,"abstract":"<div><div>Exosome microRNAs are vital for gene transcription and expression, influencing target genes. They may play direct and indirect roles in pregnancy across animal models and humans. The objective of this research was to comprehensively explore the expression profiles of plasma exosomal miRNAs during the early pregnancy stage in sheep. This study specifically delved into the differences in the expression of plasma exosomal miRNAs between Chinese Merino and Suffolk sheep. The primary focus was on clearly elucidating the changes in plasma exosomal miRNAs, pinpointing potential biomarkers that might exert an impact on early pregnancy, and thoroughly exploring their biological functions as well as the possible underlying molecular mechanisms. Chinese Merino and Suffolk sheep were divided into two groups of 20 for artificial insemination. Blood samples were collected at various pregnancy stages (day 0, day 14, and day 30), Ultrasound diagnosis was carried out five weeks following artificial insemination. Three pregnant sheep from each group were selected for isolating extracellular vesicle-derived miRNAs from plasma. High-throughput sequencing identified differentially expressed miRNAs, and their target genes were analyzed using bioinformatics methods. In plasma exosomal samples derived from all sheep of both breeds at various time points during the early pregnancy stage, a cumulative total of 80 known miRNAs and 182 novel miRNAs were successfully identified. Compared to day 0 gestation controls, both sheep breeds showed differential expression of six known miRNAs: four were up- regulated and two down- regulated. Pathway analysis linked these miRNAs to biological processes such as ECM receptor interactions, growth hormone synthesis, carbohydrate digestion, adrenergic signaling in cardiomyocytes, and MAPK signaling regulation. Notably, Oar-mir-27a differed between the two groups at various time points. In summary, this research meticulously carried out a profiling analysis of the expression of plasma exosomal miRNAs in Chinese Merino and Suffolk sheep. Through this analysis, several exosomal miRNAs were identified, which display distinct abundance levels during the early pregnancy period. These findings strongly imply that these miRNAs have the potential to serve as biomarkers for precisely assessing the reproductive status of sheep.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"276 ","pages":"Article 107833"},"PeriodicalIF":2.2,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143851562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome analysis of the ovary and testis of the pearl oyster Pinctada fucata: Identification of genes and pathways involved in gonadal development","authors":"Ying Guo ,&nbsp;Pei Wang ,&nbsp;Ruolin Zhu ,&nbsp;Tangrongjun Guo ,&nbsp;Weiwei Chen ,&nbsp;Xiuxiu Sang ,&nbsp;Lirong Bai ,&nbsp;Xianwei Fan ,&nbsp;Dahui Yu","doi":"10.1016/j.anireprosci.2025.107832","DOIUrl":"10.1016/j.anireprosci.2025.107832","url":null,"abstract":"<div><div><em>Pinctada fucata</em> is a commercially vital species in global pearl aquaculture, producing high-quality pearls. To investigate the molecular regulatory mechanisms of gonadal development in <em>P. fucata</em>, transcriptome analysis was employed to compare expression profiles between testis and ovary across four key developmental stages. A total of 56.86 Gb of clean data was generated, including 392,510 circular consensus sequencing reads, among which 292,295 full-length non-chimeric (FLNC) sequences were identified. A transcript clustering analysis of FLNC reads revealed 89,645 high-quality consensus sequences. 17,646 gene loci were identified, including 8588 novel loci and 28,121 newly discovered transcripts, of which 17,350 were successfully annotated. The boundaries of 12,040 genes on the chromosomes were corrected, and 10,761 complete open reading frame sequences. 281 genes related to gonadal development were identified, including 186 genes with full-length cDNAs in the PacBio library. The study found that <em>HUS1-like</em>, <em>MAD2A-X1</em>, and <em>BLM</em> were stage-specifically upregulated during ovarian maturation, ensuring the accuracy of meiosis. Meanwhile, <em>NR0B1</em>, <em>ETV7L-X5</em>, and <em>CAPRIN1-X2</em> promoted testicular maturation by regulating somatic cell differentiation and the germ cell microenvironment. KEGG enrichment analysis identified key pathways involved in gonadal development, including the ribosome, oxidative phosphorylation, DNA replication, and lysosome. Fatty acid metabolism was linked to ovarian maturation, while the FoxO and ErbB signaling pathways were associated with testicular maturation. These findings offer valuable insights into the molecular mechanisms regulating gonadal development in <em>P. fucata</em> and enhance genomic resources for this economically important species.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"276 ","pages":"Article 107832"},"PeriodicalIF":2.2,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143739977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AMPKα alleviates the inhibitory effect of NEFA on the function of bovine follicular granulosa cells cultured in vitro","authors":"Chang Zhao ,&nbsp;Ruru Xu ,&nbsp;Shuzhen Xin ,&nbsp;Benzheng Jiang ,&nbsp;Shibin Feng ,&nbsp;Xichun Wang ,&nbsp;Cheng Xia","doi":"10.1016/j.anireprosci.2025.107831","DOIUrl":"10.1016/j.anireprosci.2025.107831","url":null,"abstract":"<div><div>High levels of non-esterified fatty acids (NEFA) in cows with subclinical ketosis (SCK) impair postpartum follicular development and disrupt estrus. The precise mechanism through which NEFA impacts the functionality of bovine follicular cells remains elusive. An in vivo experiment was conducted to compare SCK cows without estrus (SCK-E, n = 6) with healthy cows in estrus (C-E, n = 6). In the vitro test, bovine granulosa cells (GCs) were exposed to 0.4 mM NEFA. Notably, the SCK-E group exhibited an elevated ratio of phosphorylated adenosine 5’-monophosphate-activated protein kinase α (AMPKα) to total AMPKα in both liver and ovarian tissues, compared to the C-E group. NEFA treatment of GCs adversely affected steroid hormone synthesis, suppressed the expression of cyclin and proteins crucial for steroid synthesis, and triggered cell apoptosis, thereby inhibiting cell proliferation. Furthermore, it led to a decline in cell mitochondrial membrane potential and an increase in reactive oxygen species production, ultimately causing cellular damage. Subsequently, GCs were co-cultured with adenovirus (ad-AMPKα-siRNA) and NEFA (0.4 mM). Inhibiting AMPKα further exacerbated the detrimental effects of NEFA on steroid hormone synthesis, cell apoptosis, cell proliferation, and mitochondrial function in GCs. Furthermore, upon inhibiting AMPKα, a reduction was observed in both mRNA and protein levels of acetyl-CoA carboxylase 1, accompanied by an elevation in the levels of carnitine palmitoyltransferase-1. These findings suggest that AMPKα becomes activated in SCK cows experiencing elevated NEFA levels, and that AMPKα has the potential to mitigate the detrimental effects of NEFA on GCs function in vitro.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"276 ","pages":"Article 107831"},"PeriodicalIF":2.2,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143734525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Successive seminal collections from Piaractus mesopotamicus in biofloc technology during the reproductive period","authors":"Louise Nex Spica ,&nbsp;Ruy Alberto Caetano Corrêa Filho ,&nbsp;Maurício Gustavo Coelho Emerenciano ,&nbsp;Diógenes Henrique de Siqueira Silva ,&nbsp;Lucas de Oliveira Brasileiro ,&nbsp;Rebeca Maria Sousa ,&nbsp;Giovanna Rodrigues Stringhetta ,&nbsp;Jany Bernardino Santos Gomes ,&nbsp;Adryadine Almeida da Costa ,&nbsp;Lais Santos Roque ,&nbsp;Sara Ferreira Pedrosa ,&nbsp;Jayme Aparecido Povh","doi":"10.1016/j.anireprosci.2025.107821","DOIUrl":"10.1016/j.anireprosci.2025.107821","url":null,"abstract":"<div><div>This study aimed to evaluate the sperm characteristics of pacu (<em>Piaractus mesopotamicus</em>) maintained in biofloc technology (BFT) in comparison partial water renewal system (PWR). Twenty-five males in two production systems were used: 12 in BFT and 13 in PWR. The fish were induced to reproduce in two moments: before the distribution in the experimental units of both systems; and after maintenance of the breeders for 42 days in the systems. After 42 days, only 23 % of PWR fish released semen, compared to 75 % of those kept in BFT. This improvement in the reproduction of pacu broodstock kept in BFT can be corroborated by the higher (<em>P</em> &lt; 0.05) gonadosomatic index of broodstock kept in BFT (0.387) compared to those kept in PWR (0.135), and also due to more advanced germ cell stages (spermatocytes, spermatids, spermatozoa) in broodstock testes maintained in BFT at 42 days. Despite a decrease in sperm concentration in BFT from the first to the second collection, the total number of spermatozoa did not differ significantly. Plasma membrane integrity associated with mitochondrial potential was worse for broodstock kept in PWR compared to those kept in BFT. The total amount of spermatozoa produced per liter of water used in BFT was 46,406, surpassing 304 of PWR's system. Maintaining pacu breeders in BFT for 42 days is more efficient for induced reproduction in situations of successive semen collections compared to the PWR system, with improved water optimization.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"276 ","pages":"Article 107821"},"PeriodicalIF":2.2,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143683362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relaxin-like gonad-stimulating peptide promotes gamete maturation and spawning in the sea cucumber Apostichopus japonicus","authors":"Jiaze Wang ,&nbsp;Zhaoming Gao ,&nbsp;Shuai Liu ,&nbsp;Yongchao Hu ,&nbsp;Libin Zhang ,&nbsp;Beini Deng ,&nbsp;Zhenxia Sha ,&nbsp;Xiaoshang Ru","doi":"10.1016/j.anireprosci.2025.107820","DOIUrl":"10.1016/j.anireprosci.2025.107820","url":null,"abstract":"<div><div><em>Apostichopus japonicus</em> is a crucial aquaculture species in Asia, and seedling production is fundamental to its cultivation. Nevertheless, the reproductive behavior of this species remains difficult to regulate artificially. Although the spawning mode of sea cucumbers is well documented, the endocrine mechanisms that regulate the synchronized processes of oocyte maturation and spawning behavior remain poorly understood. In this study, the effects of a recombinant relaxin-like gonad-stimulating peptide (RGP) on oocyte maturation and parental spawning behavior were investigated. The recombinant RGP derived from <em>A. japonicus</em> was effectively expressed using the <em>Pichia pastoris</em> system, and its biological activity was validated through mass spectrometry analysis. Results indicated that the RGP promoted oocyte maturation by inducing the rupture of germinal vesicles. Behavioral studies revealed that the RGP enhanced gamete release. Furthermore, the gametes induced by the RGP did not differ significantly from those released spontaneously in terms of progeny quality and quantity. These findings suggested that the recombinant RGP could be utilized in the breeding of <em>A. japonicus</em> seedlings. They also had substantial implications for elucidating the reproductive behavior of this species and for the artificial induction of seedling production.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"275 ","pages":"Article 107820"},"PeriodicalIF":2.2,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143591694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of hCG administered 5 or 7 days after the onset of induced estrus on luteal morphology and function in seasonally anovular dairy goats","authors":"Juliana Nascimento Duarte Rodrigues ,&nbsp;José Domingos Guimarães ,&nbsp;Paulo Sergio Cerqueira Rangel ,&nbsp;Maria Emilia Franco Oliveira ,&nbsp;Felipe Zandonadi Brandão ,&nbsp;Pawel Mieczyslaw Bartlewski ,&nbsp;Jeferson Ferreira Fonseca","doi":"10.1016/j.anireprosci.2025.107818","DOIUrl":"10.1016/j.anireprosci.2025.107818","url":null,"abstract":"<div><div>The objective was to evaluate the effects of intramuscular (i.m.) human chorionic gonadotropin (hCG) administered on day 5 (D5) or day 7 (D7) after estrus (D0) on luteal structures (number, area, vascularization, and progesterone secretion) and pregnancy rates in estrus-induced dairy goats during the non-breeding season. Thirty-four Alpine goats received intravaginal sponges containing medroxyprogesterone acetate for 6 days and a single i.m. injection of 200 international units (IU) of equine chorionic gonadotropin (eCG) plus 37.5 micrograms (µg) of d-cloprostenol 24 hours before sponge removal. All exhibited estrus and were mated by fertile bucks. Goats were randomly assigned to hCG-5 (300 IU on D5; n = 12), hCG-7 (300 IU on D7; n = 11), or controls (n = 11). Transrectal ovarian ultrasonography and blood sampling were performed on D5, D7, D10, D13, D17, and D21, with pregnancy diagnosis on D60. hCG treatment did not affect pregnancy rates. Accessory corpora lutea (aCL) appeared in 66.6 % (hCG-5) and 90.9 % (hCG-7) (P &gt; 0.05). Total luteal area in hCG-7 was greater (P &lt; 0.05) than in controls from D10 to D21. The mean original corpora lutea (oCL) area in hCG-7 was largest (P &lt; 0.05) on D10. High-velocity Doppler area (HVDA) of aCL was greater (P &lt; 0.05) in hCG-5 compared with hCG-7 on D13. Plasma progesterone (P4) concentrations were higher (P &lt; 0.05) in hCG-7 than in controls from D10 to D21. Although hCG administered on D5 or D7 transiently enhanced luteal function and vascularization, it did not improve pregnancy outcomes in seasonally anovular dairy goats.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"275 ","pages":"Article 107818"},"PeriodicalIF":2.2,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143580083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}