Animal Reproduction Science最新文献

{"title":"Cryopreservation of in vivo derived goat embryos: Influence of post-collection incubation methods on cellular stress and survival rates","authors":"Ribrio Ivan Tavares Pereira Batista ,&nbsp;Luana Rangel Côrtes ,&nbsp;Viviane Lopes Brair ,&nbsp;Jeferson Ferreira da Fonseca ,&nbsp;Joanna Maria Gonçalves Souza-Fabjan","doi":"10.1016/j.anireprosci.2025.107957","DOIUrl":"10.1016/j.anireprosci.2025.107957","url":null,"abstract":"<div><div>This study evaluated how post-collection conditions affect the survival of in vivo-derived (IVD) goat embryos after cryopreservation. A total of 223 IVD embryos (grades I and II) from three replicates were distributed into three groups: P0–3H, embryos maintained for 3 h at 37 °C in culture dishes with 2 mL of holding medium (HM); P6–8H, embryos held under the same conditions for 6–8 h; and T6–8H, embryos stored in cryotubes with 2 mL of HM at 37 °C for 6–8 h in a portable incubator. Osmolarity analysis showed increases of 15.3 % ± 3.4 and 48.8 % ± 8.5 in P0–3H and P6–8H, respectively, while T6–8H maintained osmotic stability (1.0 % ± 1.4). Developmental outcomes were better in T6–8H, with higher proportions of blastocysts and expanded blastocysts (27.5 %± 2.7 and 42.5 % ± 3.2) compared to P6–8H (14.1 % ± 1.4 and 34.6 % ± 3.0). However, post-thaw survival rates were similar across groups (P &gt; 0.05). Gene expression analysis revealed increased stress (PRDX1, HSP70) and apoptosis (BAX) markers in P6–8H compared to T6–8H. The highest mitochondrial activity and ROS levels were observed in T6–8H, whereas GSH levels were elevated in P6–8H, matching P0–3H values. These findings suggest that the T6–8H condition—embryos stored in cryotubes with HM at 37 °C—preserved osmotic balance, supported better embryo development, and promoted a more favorable cellular environment. Therefore, this strategy may improve embryo quality before cryopreservation, contributing to more consistent post-thaw outcomes in goat embryo transfer programs.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"280 ","pages":"Article 107957"},"PeriodicalIF":3.3,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144723070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aquaporins in bovine male reproductive tract: From calves to reproductive bulls","authors":"Katarzyna Michałek ,&nbsp;Sławomir Zych ,&nbsp;Patrycja Oberska - Łuszczewska ,&nbsp;Marta Marynowska ,&nbsp;Maciej Murawski ,&nbsp;Dariusz Gączarzewicz ,&nbsp;Jakub Andruszkiewicz ,&nbsp;Nicola Oster ,&nbsp;Andrzej Syczewski ,&nbsp;Marta Grabowska","doi":"10.1016/j.anireprosci.2025.107956","DOIUrl":"10.1016/j.anireprosci.2025.107956","url":null,"abstract":"<div><div>The role of aquaporins (AQPs) in the male reproductive system of cattle, particularly in relation to spermatogenesis, remains incompletely understood. Therefore, the aim of this study was to analyze the developmental changes in AQP8 and AQP11 expression in the bovine (Bos taurus) male reproductive tract. Tissue samples were collected from three age groups of animals: calves aged 5–6 weeks (n = 10), young bulls aged 15–25 weeks (n = 10) and reproductive bulls aged 2–6 years (n = 11). The expression of the studied AQPs was analyzed using immunohistochemistry (IHC). The identification of AQP8 and AQP11 in specific sections of the reproductive organs was verified in the oldest group of animals using PCR. The key achievements of this study include the localization of AQP8 in gonocytes, Sertoli cells, and Leydig cells. AQP8 and AQP11 were observed in the epithelium lining the rete testis, efferent ducts, successive segments of the epididymis, and vas deferens, with expression and distribution patterns changing with animal growth and development. The present results were complemented and discussed in the context of observations from our previous works. It was demonstrated that AQP expression patterns change during animal growth and development, with the frequently diffused and heterogeneous location observed in the reproductive organs of sexually immature animals, transitioning to defined and consistent patterns in mature males.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"279 ","pages":"Article 107956"},"PeriodicalIF":2.2,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144696627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptional assessment of pro-inflammatory and immune markers in the endometrium of bitches affected with CEH-Pyometra","authors":"Ajeet Kumar ,&nbsp;Deepak Kumar ,&nbsp;J.K. Prasad ,&nbsp;Ajeet Kumar ,&nbsp;Amrita Behera ,&nbsp;Alok Kumar","doi":"10.1016/j.anireprosci.2025.107952","DOIUrl":"10.1016/j.anireprosci.2025.107952","url":null,"abstract":"<div><div>The canine cystic endometrial hyperplasia (CEH)-pyometra is one of the prevalent uterine pathological conditions associated with infertility and often turn fatal. The study aimed to investigate pro-inflammatory and immune mediator transcripts in endometrium along with haematological and histopathological evaluation in healthy and clinically diagnosed CEH-pyometra conditions. The current study involved total 20 bitches from various breeds, weighing between 25 and 45 kg and aged 2–6 years. Animals under study were categorised in 3 different groups, HC group (n = 10) as healthy, CP group (n = 6) closed cervix CEH-pyometra and OP group (n = 4) as open cervix CEH-pyometra. Each animal in the study received a standardised clinical evaluation, followed by an ultra-sonographic assessment and vaginal cytology for tentative diagnosis. Blood samples were collected on the day of diagnosis. Endometrial samples were obtained by incising both uterine horns, followed by scraping endometrial part through forceps and subsequently used in evaluation of transcriptional profile. Similarly, uterine samples intended for histopathological examinations were collected by cutting 1 cm² full-thickness area. Significant (P &lt; 0.05) elevations in total leukocyte counts and neutrophilia were reported in the CP group compared to HC group. Histopathological examination revealed marked endometrial glandular hyperplasia, luminal exudation, and neutrophilic infiltration in CEH-pyometra cases, with greater severity in closed-cervix conditions. Endometrial transcript of IL 6, IL-1β, TNF-α and TGF-β1 gene in CP group animals were upregulated in significant (p &lt; 0.001) manner as compared with HC group. The elevated transcripts of pro inflammatory and immune mediators, in particular, highlight their potential roles in the inflammatory response associated with closed cervix CEH-pyometra condition. Notable variation in immunological activity based on cervical patency was evident in our study. The lack of significant rise in these pro-inflammatory markers in open cervix-pyometra condition further indicates possibility of more controlled and resolved inflammatory environment.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"279 ","pages":"Article 107952"},"PeriodicalIF":2.2,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144696626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Risk factors for Serratia marcescens contamination in liquid preserved boar semen – A cross-laboratory comparative study","authors":"Sophie Henneberg ,&nbsp;Anine Riedel ,&nbsp;Markus Jung ,&nbsp;Martin Schulze","doi":"10.1016/j.anireprosci.2025.107955","DOIUrl":"10.1016/j.anireprosci.2025.107955","url":null,"abstract":"<div><div>Antibiotic-free extenders are being used with increasing frequency in boar artificial insemination (AI) centers to prevent the promotion of antibiotic resistance. In this context, it becomes imperative to ensure hygienic semen production and to conduct regular hygiene checks in semen-processing laboratories (labs). This study presents the spermatological and microbiological findings from hygiene checks at eight German boar AI centers in 2024. At each of the 10 included labs, microbiological samples were taken from nine hygienic critical control points (HCCPs, n = 90), and 15 extended semen doses (n = 150) were examined spermatologically and microbiologically. The bacteria were identified based on matrix-assisted laser desorption ionization-time of flight mass spectrometry. Additionally, antimicrobial susceptibility tests were performed for 13 commonly used antibiotics. In total, 21 different bacterial genera were identified at the HCCPs. Contamination with <em>Serratia marcescens</em> (<em>S. marcescens</em>) was found in three labs. In two labs, the sinks were contaminated and in one lab, the semen dyes were contaminated with <em>S. marcescens</em>. A total of 15 semen doses (10 %) from four AI centers were found to be contaminated with bacteria, 11 contained <em>S. marcescens.</em> The contamination was associated with a decline in sperm quality, characterized by sperm agglutination, and reduced total sperm motility after thermo-resistance test. The antibiogram revealed a resistance of <em>S. marcescens</em> isolates to 9 ± 1 (mean ± SD) of 13 tested antibiotics, particularly gentamicin. Considering the findings, it is recommended that labs prioritize maintaining accurate hygiene in sinks and drains, and that they consider abstaining from the use of semen dyes in AI semen production.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"279 ","pages":"Article 107955"},"PeriodicalIF":2.2,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144663469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vitamin E nanoemulsion enhances porcine sperm quality and reproductive performance via antioxidant enhancement and fatty acid metabolism regulation","authors":"Chaoyue Cao,&nbsp;Xue Qin,&nbsp;Yiwen Sha,&nbsp;Haoqi Xiao,&nbsp;Zhenheng Lai,&nbsp;Yi Zheng,&nbsp;Rui Cai,&nbsp;Weijun Pang","doi":"10.1016/j.anireprosci.2025.107954","DOIUrl":"10.1016/j.anireprosci.2025.107954","url":null,"abstract":"<div><div>Vitamin E (VE) is widely used as an antioxidant in boar semen preservation. Its low water solubility and limited bioavailability, however, compromise its functional efficacy during storage. To address these limitations, we developed a lecithin-stabilized vitamin E nanoemulsion (NE) to enhance its dispersibility and antioxidant efficacy. Semen from Duroc boars was diluted in Beltsville thawing solution (BTS) containing either 0.6 mM NE, 4 mM free VE, or no additive, and stored at 17 °C. Compared to VE and control groups, NE significantly enhanced sperm motility, preserved the structural integrity of the plasma and acrosomal membranes, and maintained mitochondrial function, while reducing oxidative stress and DNA fragmentation over a 7d storage period (<em>P</em> &lt; 0.01). Untargeted metabolomics revealed that NE supplementation regulated key pathways including unsaturated fatty acid metabolism and glutathione-related antioxidation. Artificial insemination (AI) trials involving 637 sows confirmed that NE-treated semen improved total and healthy piglet numbers per litter (<em>P</em> &lt; 0.05), without adverse effects. These findings suggest that NE provides an effective and practical strategy for enhancing boar semen preservation and reproductive outcomes in porcine production.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"279 ","pages":"Article 107954"},"PeriodicalIF":2.2,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144663520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel microfluidics-based device achieves automatic cumulus cells removal and optimal embryo development in cattle","authors":"Javier Guerrero-Sánchez ,&nbsp;Gonzalo Fernández-Blanco ,&nbsp;Ismael Lamas-Toranzo ,&nbsp;Leopoldo González-Brusi ,&nbsp;Alba Pérez-Gómez ,&nbsp;Beatriz Galiano-Cogolludo ,&nbsp;Ana Querejeta-Fernández ,&nbsp;Yolanda Cabello ,&nbsp;Joana Fidalgo ,&nbsp;Lionel Matthys ,&nbsp;Santiago Munné ,&nbsp;José A. Horcajadas ,&nbsp;Pablo Bermejo-Álvarez","doi":"10.1016/j.anireprosci.2025.107953","DOIUrl":"10.1016/j.anireprosci.2025.107953","url":null,"abstract":"<div><div>Oocyte or zygote denudation is a procedure required for optimal visualization of the oocyte or the developing embryo. Cumulus cells are conventionally removed by mechanical and/or enzymatic treatment, a manual procedure prone to operator-to-operator variations in completeness of denudation and shear force applied. We have developed an automated denudation prototype based on microfluidics able to denude 16 bovine cumulus oocyte complexes (COCs) at a time. COCs matured and fertilized <em>in vitro</em> were randomly allocated into two groups: one was denuded by manual stripping and other was denuded using the automatic denudation prototype. A zona pellucida permeability test observed that zonae were permeable to 20 nm particles and impermeable to 40 and 100 nm, irrespective of the denudation protocol employed. Blastocyst formation rates were similar between both protocols, and no differences were observed in the total, inner cell mass and trophectoderm cell numbers at the blastocyst stage. Blastocyst transcriptome was remarkably similar between both denudation groups, with only three genes being differentially expressed. In contrast, 36 and 48 genes were differentially expressed between <em>in vivo</em> derived blastocysts and <em>in vitro</em> produced blastocysts following automatic and manual denudation, respectively. Finally, an <em>in vitro</em> analysis of post-hatching developmental ability revealed similar survival, hypoblast migration and embryonic disc formation rates between embryos obtained following manual or automatic denudation. In conclusion, the device possibilities a safe and repeatable means to denude bovine COCs and constitutes a first step to attain automatic denudation in human COCs, whose size is remarkably similar to those of cattle.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"279 ","pages":"Article 107953"},"PeriodicalIF":2.2,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144663468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of long-acting recombinant porcine FSH on reproductive performance and production efficiency of gilts and weaned sows","authors":"Jiawei Zhang ,&nbsp;Lei Shi ,&nbsp;Zheng Huang ,&nbsp;Shiwen Cheng ,&nbsp;Guo Han ,&nbsp;Jiansheng Xiong ,&nbsp;Jiahua Bai ,&nbsp;Shuyu Xie ,&nbsp;Dongmei Chen ,&nbsp;Yan Liu ,&nbsp;Haoshu Luo","doi":"10.1016/j.anireprosci.2025.107951","DOIUrl":"10.1016/j.anireprosci.2025.107951","url":null,"abstract":"<div><div>To enhance production efficiency, equine chorionic gonadotropin (eCG) plays a crucial role in promoting follicular development in the reproductive management of sows; however, eCG is a heterologous protein derived from pregnant mare serum. It is essential to develop and utilize more advantageous alternative products in the reproductive management of sows. In this study, a novel long-acting recombinant porcine FSH (rpFSH-pFc) was used for the first time to promote synchronized estrus in sows. Through dose screening, we found that a combination of 40 μg of rpFSH-pFc and 200 IU of hCG effectively promoted synchronized estrus in gilts. Moreover, this combination could effectively enhance the secretion of estradiol and progesterone in gilts. Subsequently, this combination was applied to synchronize estrus in both gilts and weaned sows. Our results demonstrated that the combination hormone treatment group had a significantly higher number of piglets born alive per litter compared to the control group (12.0 ± 1.9 vs. 10.6 ± 1.7, <em>P</em> &lt; 0.05). Although the treatment group receiving the combination of rpFSH-pFc and hCG exhibited outcomes similar to those of the eCG treatment group, it resulted in a greater total number of piglets in both gilts (192 vs. 176) and weaned sows (531 vs. 481), compared to the eCG treatment group, with equal sample sizes. Taken together, our results revealed that rpFSH-pFc might be an ideal candidate to promote the synchronization of estrus and ovulation in sows and improve the reproductive efficiency of livestock production.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"279 ","pages":"Article 107951"},"PeriodicalIF":2.2,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144631694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of the administration of dexamethasone in pregnant goats: Vitality, thermography, physiological response, and behavioral patterns of newborn kids","authors":"Daniela Bello-Cabrera ,&nbsp;Luis A. de la Cruz-Cruz ,&nbsp;Cristian Larrondo ,&nbsp;Juan M. Vázquez-García ,&nbsp;Patricia Roldán-Santiago","doi":"10.1016/j.anireprosci.2025.107949","DOIUrl":"10.1016/j.anireprosci.2025.107949","url":null,"abstract":"<div><div>Perinatal deaths cause economic losses in goat production, and birth induction has emerged as a key strategy for producers to plan parturition periods and thus increase neonatal survival. The objective of this study was to evaluate the effects of dexamethasone administration in pregnant goats on the vitality, thermographic profile, physiological response, and behavioral patterns of their newborn kids. A total of 36 multiparous French Alpine goats and their offspring were randomly assigned into three groups: G1, control goats; G2, goats that received a 0.9 % intramuscular injection of physiological saline solution 36 h before parturition; and G3, goats that received a 16-mg intramuscular injection of dexamethasone 36 h prior to the estimated parturition date. In the dams, the duration of labor, interval between expulsions, latency to first grooming, sniffing, surface temperature, and mother–young bonding were evaluated. In the kids, vitality, neonatal behavior, blood glucose and lactate levels, oxygenation saturation, birth weight, and surface temperature were evaluated at birth, after the first suckling, and at 24 h postpartum. Kids born to dexamethasone-treated goats showed improved vitality parameters compared to neonates from untreated dams, particularly in the suckling reflex (P &lt; 0.0001) and in normal oxygenation and glucose levels. Additionally, dexamethasone-treated dams showed improved maternal behavior. We conclude that administering a 16-mg dose of dexamethasone to naturally mated goats improves mother–kid bonding and results in kids born with higher surface temperatures, adequate oxygenation levels, and higher percentages of the suckling reflex.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"279 ","pages":"Article 107949"},"PeriodicalIF":2.2,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144587594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of simultaneous addition of cyanidin 3-glucoside and cyanidin 3-rutinoside in boar semen stored under refrigeration at 17°C for 168 hours","authors":"Henricco Zapparoli e Silva ,&nbsp;Guilherme Ferreira da Silva ,&nbsp;Rosa Daniela Palchucan Nieto ,&nbsp;Ana Carolina Pedrosa ,&nbsp;Simone Maria Massami Kitamura Martins ,&nbsp;André Furugen Cesar de Andrade","doi":"10.1016/j.anireprosci.2025.107930","DOIUrl":"10.1016/j.anireprosci.2025.107930","url":null,"abstract":"","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"278 ","pages":"Article 107930"},"PeriodicalIF":2.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144569779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of glycolytic and non-glycolytic substrates on the liquid preservation of pig semen","authors":"Ferran Garriga ,&nbsp;Marc Llavanera ,&nbsp;Jodd Codina-Benaiges ,&nbsp;Marc Yeste ,&nbsp;Joan E. Rodríguez-Gil","doi":"10.1016/j.anireprosci.2025.107944","DOIUrl":"10.1016/j.anireprosci.2025.107944","url":null,"abstract":"","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"278 ","pages":"Article 107944"},"PeriodicalIF":2.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144569979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}