Animal Reproduction Science最新文献

{"title":"Relaxin-like gonad-stimulating peptide promotes gamete maturation and spawning in the sea cucumber Apostichopus japonicus","authors":"Jiaze Wang ,&nbsp;Zhaoming Gao ,&nbsp;Shuai Liu ,&nbsp;Yongchao Hu ,&nbsp;Libin Zhang ,&nbsp;Beini Deng ,&nbsp;Zhenxia Sha ,&nbsp;Xiaoshang Ru","doi":"10.1016/j.anireprosci.2025.107820","DOIUrl":"10.1016/j.anireprosci.2025.107820","url":null,"abstract":"<div><div><em>Apostichopus japonicus</em> is a crucial aquaculture species in Asia, and seedling production is fundamental to its cultivation. Nevertheless, the reproductive behavior of this species remains difficult to regulate artificially. Although the spawning mode of sea cucumbers is well documented, the endocrine mechanisms that regulate the synchronized processes of oocyte maturation and spawning behavior remain poorly understood. In this study, the effects of a recombinant relaxin-like gonad-stimulating peptide (RGP) on oocyte maturation and parental spawning behavior were investigated. The recombinant RGP derived from <em>A. japonicus</em> was effectively expressed using the <em>Pichia pastoris</em> system, and its biological activity was validated through mass spectrometry analysis. Results indicated that the RGP promoted oocyte maturation by inducing the rupture of germinal vesicles. Behavioral studies revealed that the RGP enhanced gamete release. Furthermore, the gametes induced by the RGP did not differ significantly from those released spontaneously in terms of progeny quality and quantity. These findings suggested that the recombinant RGP could be utilized in the breeding of <em>A. japonicus</em> seedlings. They also had substantial implications for elucidating the reproductive behavior of this species and for the artificial induction of seedling production.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"275 ","pages":"Article 107820"},"PeriodicalIF":2.2,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143591694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of hCG administered 5 or 7 days after the onset of induced estrus on luteal morphology and function in seasonally anovular dairy goats","authors":"Juliana Nascimento Duarte Rodrigues ,&nbsp;José Domingos Guimarães ,&nbsp;Paulo Sergio Cerqueira Rangel ,&nbsp;Maria Emilia Franco Oliveira ,&nbsp;Felipe Zandonadi Brandão ,&nbsp;Pawel Mieczyslaw Bartlewski ,&nbsp;Jeferson Ferreira Fonseca","doi":"10.1016/j.anireprosci.2025.107818","DOIUrl":"10.1016/j.anireprosci.2025.107818","url":null,"abstract":"<div><div>The objective was to evaluate the effects of intramuscular (i.m.) human chorionic gonadotropin (hCG) administered on day 5 (D5) or day 7 (D7) after estrus (D0) on luteal structures (number, area, vascularization, and progesterone secretion) and pregnancy rates in estrus-induced dairy goats during the non-breeding season. Thirty-four Alpine goats received intravaginal sponges containing medroxyprogesterone acetate for 6 days and a single i.m. injection of 200 international units (IU) of equine chorionic gonadotropin (eCG) plus 37.5 micrograms (µg) of d-cloprostenol 24 hours before sponge removal. All exhibited estrus and were mated by fertile bucks. Goats were randomly assigned to hCG-5 (300 IU on D5; n = 12), hCG-7 (300 IU on D7; n = 11), or controls (n = 11). Transrectal ovarian ultrasonography and blood sampling were performed on D5, D7, D10, D13, D17, and D21, with pregnancy diagnosis on D60. hCG treatment did not affect pregnancy rates. Accessory corpora lutea (aCL) appeared in 66.6 % (hCG-5) and 90.9 % (hCG-7) (P &gt; 0.05). Total luteal area in hCG-7 was greater (P &lt; 0.05) than in controls from D10 to D21. The mean original corpora lutea (oCL) area in hCG-7 was largest (P &lt; 0.05) on D10. High-velocity Doppler area (HVDA) of aCL was greater (P &lt; 0.05) in hCG-5 compared with hCG-7 on D13. Plasma progesterone (P4) concentrations were higher (P &lt; 0.05) in hCG-7 than in controls from D10 to D21. Although hCG administered on D5 or D7 transiently enhanced luteal function and vascularization, it did not improve pregnancy outcomes in seasonally anovular dairy goats.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"275 ","pages":"Article 107818"},"PeriodicalIF":2.2,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143580083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of the reproductive cycles of two cryptic soles in the Adriatic Sea","authors":"Laura Sabatini ,&nbsp;Fortunata Donato ,&nbsp;Mario La Mesa ,&nbsp;Emanuela Fanelli ,&nbsp;Giuseppe Scarcella","doi":"10.1016/j.anireprosci.2025.107817","DOIUrl":"10.1016/j.anireprosci.2025.107817","url":null,"abstract":"<div><div>In the Adriatic Sea, two congeneric and cryptic soles coexist and share the same habitats. They are the common sole, <em>Solea solea</em>, one of the main Mediterranean fishery resources, and the Egyptian sole, <em>Solea aegyptiaca</em>. The present study investigated and compared their reproductive biology to obtain updated and original information useful for explaining their coexistence as distinct and reproductively isolated species and supporting stock assessment and management measures. The comparison of the reproductive cycle and strategy revealed similar and distinctive traits. Histological analysis indicated that both species had asynchronous ovaries and were batch-spawners. On the other hand, spawning activities occurred in late autumn or late winter-early spring in <em>S. solea</em> and <em>S. aegyptiaca</em>, respectively. Such a different reproductive timing may contribute to their sexual isolation and genetic distinction. Other pre-zygotic isolating processes have been hypothesized to act by preventing mating, such as the involvement of olfactory sense for intraspecific recognition. Fecundity estimates were similar between the two species (mean potential fecundity: 323,122 for <em>S. aegyptiaca</em> and 210,752 for <em>S. solea</em>, <em>P</em> &gt; 0.05), but the oocytes produced by <em>S. aegyptiaca</em> were significantly smaller than those of <em>S. solea</em>. This study contributed to shedding light on the reproductive traits that may support the coexistence of these congeneric species and encouraged future investigations to understand their fitness in the Adriatic Sea.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"275 ","pages":"Article 107817"},"PeriodicalIF":2.2,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143644451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant enzyme dynamics suggest the absence of oxidative stress in the canine endometrium across the estrous cycle","authors":"R. Payan-Carreira ,&nbsp;C. Santos ,&nbsp;D. Santos","doi":"10.1016/j.anireprosci.2025.107819","DOIUrl":"10.1016/j.anireprosci.2025.107819","url":null,"abstract":"<div><div>Oxidative stress plays a vital role in female fertility, yet the mechanisms regulating oxidative balance in the canine endometrium remain poorly understood. This study investigates the dynamics of the antioxidant enzyme system in the canine endometrium, focusing on superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), glutathione reductase (GSR), and glutathione-S-transferase (GST), along with thiobarbituric acid reactive substances (TBARS) and total cellular thiols. Enzyme activities were revealed in five different phases of oestrus cycle in 25 dogs, using a spectrophotometric method: anestrus, proestrus, estrus, early diestrus and diestrus (n = 5 per group). Notably, a distinctive pattern in SOD and CAT activity was observed, with the former being characterised by a decrease from anestrus to estrus, and the later showed an opposite increase from anestrus to diestrus. In contrast, the activities of the glutathione-dependent enzymes GPX, GSR, and GST remained remarkably stable, although showing some fluctuations in different stages. TBARS analysis indicated an evident increase in oxidative stress-related lipid peroxidation in the canine endometrium only between anestrus and proestrus. Conversely, the thiol cell content remained consistent within the cycle stages. Our examination of enzyme ratios underscores a delicate balance in the normal canine uterus, effectively controlling oxidative stress without causing damage to lipids or proteins due to excessive reactive oxygen species. These findings contribute to our understanding of the unique physiological dynamics of the canine endometrium, offering valuable insights into the intricate regulation of oxidative stress in this context and its potential implications for female fertility.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"275 ","pages":"Article 107819"},"PeriodicalIF":2.2,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143552836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gelatin capsules for post mortem vitrification of Piracanjuba (Brycon orbignyanus) ovarian tissue as an accessible solution for emergency genetic preservation","authors":"Thaiza Rodrigues de Freitas ,&nbsp;Lis Santos Marques ,&nbsp;Raquel Santos dos Santos ,&nbsp;Renata Villar Dantas ,&nbsp;Thales Souza França ,&nbsp;Rômulo Batista Rodrigues ,&nbsp;Karel Gelina Torres-Lozano ,&nbsp;Eduardo Albuquerque ,&nbsp;Diógenes Henrique Siqueira-Silva ,&nbsp;Tiantian Zhang ,&nbsp;Danilo Pedro Streit Jr","doi":"10.1016/j.anireprosci.2025.107816","DOIUrl":"10.1016/j.anireprosci.2025.107816","url":null,"abstract":"<div><div>Environmental disasters have caused significant losses in fish populations, threatening the genetic diversity of many species. Cryopreservation of genetic material post-mortem has emerged as a potential solution to safeguard biodiversity. However, a fast, accessible method for cryopreserving such material in emergencies is needed. This study aims to evaluate gelatin capsules as a practical, rapid alternative to cryovials for the vitrification of fish ovarian tissue <em>post mortem</em>, providing an emergency method for genetic preservation. Experiments were conducted during a real-life incident where fish were found deceased. Gelatin capsules demonstrated performance comparable to cryovials in terms of oocyte viability (capsules: 68.3 ± 10.3 %; cryovials: 64 ± 5.7 %) and mitochondrial activity (capsules: 10.3 ± 4.5 AU/g; cryovials: 8.7 ± 2.7 AU/g) immediately after warming. Viability decreased after 24 h of incubation, but no significant differences were observed between treatments or containers. Histological analysis revealed minor morphological damage in capsule-vitrified oocytes immediately after warming; however, damage was equally present in both containers after 24 h. The addition of LH and FSH to the medium reduced oocyte damage, while cryovial samples without hormones showed larger cell areas (0.077 ± 0.023 mm) and perimeters (3.31 ± 0.98 mm). Gelatin capsules are a viable, accessible option for emergency cryopreservation, serving as a useful tool for preserving maternal fish genetic material under urgent circumstances. Additionally, viable primary growth oocytes were successfully obtained <em>post mortem</em> and remained viable after 24 h of incubation, highlighting the potential of this method for emergency genetic preservation applications.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"275 ","pages":"Article 107816"},"PeriodicalIF":2.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143552835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A metabolomic study uncovering key amino acids and amines in Duroc boar semen as biomarkers of sexual maturity","authors":"Asmita Shrestha ,&nbsp;Ann Helen Gaustad ,&nbsp;Janne Beate Øiaas ,&nbsp;Elisabeth Kommisrud ,&nbsp;Maren van Son ,&nbsp;Anna Nordborg ,&nbsp;Anne Hege Alm-Kristiansen","doi":"10.1016/j.anireprosci.2025.107800","DOIUrl":"10.1016/j.anireprosci.2025.107800","url":null,"abstract":"<div><div>Metabolomic analysis of boar semen associated with sexual maturation is essential for improving fertility management and breeding, with amino acids and amines playing key roles in the reproductive process. This study aimed to explore changes in amino acids and amines in boar spermatozoa and seminal plasma during puberty to sexual maturity and identify potential biomarkers of sexual maturity. Semen was collected from the same 15 Duroc boars over time at approximately 7 months (Age 1), 8.5 months (Age 2), and 10 months (Age 3). Liquid chromatography-mass spectrometry was used to analyse amino acids and amines in spermatozoa and seminal plasma separately. Multivariate analysis (PLS-DA) revealed pronounced age-dependent changes in amino acids and amines in spermatozoa between Age 1 and Age 3, and more subtle shifts in seminal plasma. Univariate analysis (Repeated measure ANOVA/Friedman) revealed that glutamate and taurine had significant pairwise differences in seminal plasma (<em>P</em> &lt; 0.05). In sperm, 15 amino acids (glutamate, alanine, aspartate, choline, taurine, histidine, methionine, tryptophan, leucine, cystine, tyrosine, arginine, lysine, valine and glycine) exhibited significant pairwise differences (<em>P</em> &lt; 0.05). VIP scoring (&gt;1.5) prioritised glutamate, alanine, aspartate, and choline as key contributors to the variations and pathway analysis implicated alanine, aspartate and glutamate metabolism, and histidine metabolism linked to sexual maturity. Our study highlights metabolic changes during sexual maturation, identifying potential biomarkers for assessing reproductive maturity. These findings are initial steps toward optimising younger boars' usage in breeding, enhancing genetic gain, and reducing costs associated with their non-productive days at AI centres.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"275 ","pages":"Article 107800"},"PeriodicalIF":2.2,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143474586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The use of wheat grass juice as a promising functional feed additive for enhancing reproductive performance and larvae quality of sterlet (Acipenser ruthenus)","authors":"C.-A. Barbacariu ,&nbsp;G. Dumitru ,&nbsp;L. Dîrvariu ,&nbsp;D.A. Şerban ,&nbsp;I. Dincheva ,&nbsp;E. Todirascu-Ciornea ,&nbsp;M. Burducea","doi":"10.1016/j.anireprosci.2025.107796","DOIUrl":"10.1016/j.anireprosci.2025.107796","url":null,"abstract":"<div><div>The optimization of broodstock nutrition remains a critical challenge in sturgeon aquaculture. This study investigated the effects of wheatgrass juice (WGJ) supplementation of sterlet (<em>Acipenser ruthenus</em>) broodstock on reproductive performance, larval quality, hematological profile and oxidative status. Female sterlets were randomly assigned to three groups: control diet (V1), WGJ-supplemented diet (V2, 4 % inclusion), both (V1 and V2) hormonally stimulated and saline solution (V3) without hormonal stimulation. WGJ's chemical composition revealed substantial levels of bioactive compounds, including chlorophyll (a: 0.936 ± 0.01, b: 0.329 ± 0.01 mg/mL fw), carotenoids (0.167 ± 0.01 mg/mL fw), proteins (1.205 ± 0.04 mg PSB/mL fw), flavonoids (7.381 ± 0.31 mg/mL fw), and polyphenols (2.963 ± 0.05 mg/mL fw). WGJ supplementation significantly enhanced reproductive parameters, with V2 showing higher egg production (0.23 ± 0.03 kg vs. 0.13 ± 0.01 kg) and gonadosomatic index (14.48 ± 1.73 vs. 8.05 ± 0.51) compared to V1. Larvae from WGJ-supplemented females exhibited significantly improved length (10.9 ± 0.3 cm vs. 9.8 ± 0.14 cm, p &lt; 0.001) and weight parameters. Analysis of oxidative stress markers revealed tissue-specific modulation of antioxidant systems, with WGJ supplementation reducing malondialdehyde levels across tissues (muscle: 1.061 ± 0.075 vs. 1.888 ± 0.105 nM/mg protein, p &lt; 0.0001) and enhancing antioxidant enzyme activities. Hematological parameters remained within physiological ranges, with reduced neutrophil counts in the WGJ group suggesting potential immunomodulatory effects. These findings establish WGJ as a promising functional feed additive for enhancing reproductive performance in sterlet aquaculture, while maintaining optimal physiological status through improved antioxidant defense mechanisms.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"274 ","pages":"Article 107796"},"PeriodicalIF":2.2,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143429542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nicotinamide mononucleotide enhances porcine sperm quality by activating the SIRT3-SOD2/ROS pathway and promoting oxidative phosphorylation","authors":"Haize Zhang,&nbsp;Xue Qin,&nbsp;Nataraj Bojan,&nbsp;Chaoyue Cao,&nbsp;Jiawen Chai,&nbsp;Weijun Pang","doi":"10.1016/j.anireprosci.2025.107797","DOIUrl":"10.1016/j.anireprosci.2025.107797","url":null,"abstract":"<div><div>Sperm quality is crucial for animal reproduction but is often deteriorates due to oxidative stress. Preserving functionality during storage by protecting it from oxidative stress is crucial for enhancing the success of assisted reproductive technologies and maintaining fertility. Nicotinamide mononucleotide (<strong>NMN</strong>), a precursor of Nicotinamide adenine dinucleotide (<strong>NAD</strong>^{<strong>+</strong>}), acts as a potent antioxidant. However, its role and regulatory mechanism in porcine sperm liquid preservation <em>in vitro</em> are remains unclear. In this study we demonstrated that NMN supplementation in the semen diluent significantly improved porcine sperm quality during storage at 17 °C by increasing NAD⁺ concentrations and decreasing the reactive oxygen species (<strong>ROS</strong>) concentrations in sperm. Subsequent analysis revealed that NMN, upon conversion to NAD⁺ within sperm, exerted antioxidant effect by upregulating the mitochondrial protein sirtuin 3 (<strong>SIRT3</strong>), resulting in the downregulation of superoxide dismutase 2 (<strong>SOD2</strong>) acetylation. This pathway mitigated oxidative stress. Additionally, NMN also enhanced sperm oxidative phosphorylation (<strong>OXPHOS</strong>) thereby increase adenosine triphosphate (<strong>ATP</strong>) production concentrations via activating SIRT3, without affecting sperm glycolysis. Furthermore, the semen diluent supplemented with 50 μg/mL NMN resulted in improved reproductive outcomes, including the birth of healthier piglets and a reduction in stillbirths rates following artificial insemination (<strong>AI</strong>). Taken together, NMN improves porcine sperm quality through SIRT3-activating the SOD2/ROS axis and oxidative phosphorylation. Our findings advance understanding of sperm physiology and metabolism and provide a new avenue promising approach for developing innovative semen diluents for liquid preservation in animal reproduction biology <em>in vitro.</em></div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"275 ","pages":"Article 107797"},"PeriodicalIF":2.2,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143428205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of dietary supplementation of Bacillus subtilis QST 713 on constipation, reproductive performance and offspring growth performance of sows","authors":"Fuyong Li ,&nbsp;Deyuan Wu ,&nbsp;Kaidi Ma ,&nbsp;Tanghong Wei ,&nbsp;Junyi Wu ,&nbsp;Shijian Zhou ,&nbsp;Shizhe Xiang ,&nbsp;Zhenpeng Zhu ,&nbsp;Xiandong Zhang ,&nbsp;Chengquan Tan ,&nbsp;Hefeng Luo ,&nbsp;Jinping Deng","doi":"10.1016/j.anireprosci.2025.107785","DOIUrl":"10.1016/j.anireprosci.2025.107785","url":null,"abstract":"<div><div>The experiment aimed to investigate the effects of dietary supplementation with <em>Bacillus subtilis</em> QST 713 (BS) on the reproductive performance, perinatal constipation, and offspring growth performance of sows. 78 multiparous Landrace-Yorkshire sows with same parity and backfat thickness were divided into 2 groups: a basal diet group (CON) and a basal diet supplemented with 1.5 × 10⁹ CFU/kg BS. The experiment was conducted from day 85 of gestation to day 21 of lactation. Reproductive performance, lactation performance, and perinatal fecal scores of sows were recorded. Samples were collected for subsequent analysis, including the feces of sows on day 110 of gestation, colostrum within 2 hours of parturition, milk on day 21 of lactation and feces from sows on day 21 of lactation. The results showed that, compared with the CON group, the BS group significantly shortened the duration of parturition (<em>P</em> &lt; 0.01) and the average birth interval of piglets (<em>P</em> &lt; 0.05), reduced stillbirth rate (<em>P</em> &lt; 0.05) and invalid piglet rate (<em>P</em> &lt; 0.05). Additionally, the BS group increased the content of protein (<em>P</em> &lt; 0.05) and level of IgM in colostrum (<em>P</em> &lt; 0.05), as well as the average daily gain (<em>P</em> &lt; 0.05) of weaned piglets. The BS group increased the perinatal fecal score (<em>P</em> &lt; 0.05) of sows, decreased the incidence of post-partum constipation (<em>P</em> &lt; 0.05), and increased the relative abundance of <em>Lactobacillus</em> (<em>P</em> &lt; 0.05) in feces on day 110 of gestation. In summary, a maternal diet supplemented with BS reduced the stillbirth rate by shortening the duration of parturition, alleviated perinatal constipation, and improved intestinal microbiota, meanwhile improving the growth performance of piglets by increasing nutrients and immunoglobulins in colostrum.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"274 ","pages":"Article 107785"},"PeriodicalIF":2.2,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143428250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of the simultaneous addition of cyanidin 3-glucoside and cyanidin 3-rutinoside in boar semen preserved under refrigeration at 17°C for 168 hours","authors":"Henricco Zapparoli ,&nbsp;Ana Carolina Pedrosa ,&nbsp;Jorgea Pradiee ,&nbsp;Guilherme Ferreira da Silva ,&nbsp;Rosa Daniela Palchucan Nieto ,&nbsp;Lourdes Maria Corrêa Cabral ,&nbsp;Flávia dos Santos Gomes ,&nbsp;Renata Valeriano Tonon ,&nbsp;Simone Maria Massami Kitamura Martins ,&nbsp;André Furugen Cesar de Andrade","doi":"10.1016/j.anireprosci.2025.107799","DOIUrl":"10.1016/j.anireprosci.2025.107799","url":null,"abstract":"<div><div>Artificial insemination is widely used in swine reproductive management, allowing a single high-genetic-value male to fertilize a larger number of females. However, the dilution of spermatozoa in cooling media does not prevent oxidative processes that reduce the fertilizing capacity of sperm cells. This study investigated the effects of the antioxidant compounds cyanidin 3-glucoside and cyanidin 3-rutinoside, present in açaí extract, on sperm motility and the integrity of the plasma and acrosomal membranes of boar spermatozoa cooled to 17 ºC for up to 168 hours and the impact of these compounds on preventing the formation of superoxide anions. Semen samples were diluted with different concentrations of these compounds and compared with a long-term extender (Vitasem®) and a negative control. The results were evaluated at three time points: initial (time 0), after 96 hours, and after 168 hours of cooling. Vitasem® maintained high levels of motility and sperm plasma membrane integrity, while the cyanidins did not provide significant improvements compared to the negative control. Statistical analysis indicated that the addition of cyanidin 3-glucoside and cyanidin 3-rutinoside at the tested concentrations did not result in significant improvements in sperm characteristics. These findings suggest the need to explore other concentrations and combinations of antioxidants to optimize sperm preservation during cold storage.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"276 ","pages":"Article 107799"},"PeriodicalIF":2.2,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143835404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}