Animal Reproduction Science最新文献_第4页

Boar semen cryopreservation: State of the art, and international trade vision 公猪精液冷冻保存：技术现状与国际贸易展望

IF 2.2 2区农林科学

Animal Reproduction Science Pub Date : 2024-10-01 DOI: 10.1016/j.anireprosci.2024.107496

{"title":"Boar semen cryopreservation: State of the art, and international trade vision","authors":"","doi":"10.1016/j.anireprosci.2024.107496","DOIUrl":"10.1016/j.anireprosci.2024.107496","url":null,"abstract":"<div><div><span>Biosecurity<span> is a major concern in the global pig<span> production. The separation in time of semen collection, processing and </span></span></span>insemination<span><span> in the pig farm is a few days for chilled semen but it can be indefinite when using cryopreserved semen. Field fertility results of boar cryopreserved semen are close to chilled semen, which makes it a valuable resource for the establishment of semen genebanks, long-distance semen trade, and the implementation of other technologies such as the sex-sorted semen. But cryopreserved semen is far from being routine in pig farms. The most recent research efforts to facilitate its implementation include the use of additives before freezing, or in the thawing extender. Long-term preserved semen trade is a biosecurity challenge. To harmonize international trade of germplasm, the World Organization of Animal Health (WOAH) established a regulatory framework for all member countries. The present paper aims to review the latest advances of boar semen </span>cryopreservation with special focus on the benefits of its inclusion as a routine tool in the pig industry. We also review recently reported field fertility results of cryopreserved semen, its international trade compared to chilled semen, and the regulatory framework involved. Boar cryopreserved semen is a valuable tool to control biosecurity risk, implement other technologies, and facilitate international trade. Research already demonstrated good field fertility results, but it still represents less than 0.1 % of the international trade. As boar cryopreserved semen gets closer to implementation, the correspondent authorities are reviewing the trade rules.</span></div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"269 ","pages":"Article 107496"},"PeriodicalIF":2.2,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141037019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multimodal distribution and its impact on the accurate assessment of spermatozoa morphological data: Lessons from machine learning 多模态分布及其对精子形态数据准确评估的影响：机器学习的启示。

IF 2.2 2区农林科学

Animal Reproduction Science Pub Date : 2024-10-01 DOI: 10.1016/j.anireprosci.2024.107564

D. Stefanovski , M. Schulze , G.C. Althouse

{"title":"Multimodal distribution and its impact on the accurate assessment of spermatozoa morphological data: Lessons from machine learning","authors":"D. Stefanovski , M. Schulze , G.C. Althouse","doi":"10.1016/j.anireprosci.2024.107564","DOIUrl":"10.1016/j.anireprosci.2024.107564","url":null,"abstract":"<div><div><span><span><span>Objective assessment of sperm morphology is an essential component for assessing ejaculate quality. Due to economic limitations, investigators often divert to conducting observational studies instead of experimental ones, which provide the strongest statistical power, yielding more heterogeneous data regardless of the number of </span>data sources (barns/farms). Using such data inevitably leads to higher variances of estimates, which negatively impacts the statistical power of a study. In this article, we describe a statistical methodology called finite mixture modeling (FMM), which, based on the supplied data and assumed number of sub-classes, classifies the data into two or more homogeneous types of distributions and determines their fractional size relative to the entire cohort. The goal is to use statistical methods that will confound the variance of the sample. A figure from a previous publication was used to generate </span>simulated data (n=1559) on the cytoplasmic droplet rate. We identified that a bi-modal distribution with two latent classes best described the simulated data. </span><em>Post-hoc</em><span> estimation showed that about 80 % of observations belonged to latent class 1, with 20 % in latent class 2. The FMM methodology identified a cutoff point of 8.7 %. Finally, when estimating the standard error for the total cohort, the FMM methodology yielded a 40 % reduction in the standard error compared to standard methodologies. In conclusion, here we show that FMM successfully confounded the variance of the data and, as such, yielded lower estimates of the variance than standard methodologies, increasing the statistical power of the cohort.</span></div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"269 ","pages":"Article 107564"},"PeriodicalIF":2.2,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141756690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pigs in heaven: Enhancing the lives of breeding boars 天堂里的猪改善种公猪的生活。

IF 2.2 2区农林科学

Animal Reproduction Science Pub Date : 2024-10-01 DOI: 10.1016/j.anireprosci.2024.107549

Pol Llonch

{"title":"Pigs in heaven: Enhancing the lives of breeding boars","authors":"Pol Llonch","doi":"10.1016/j.anireprosci.2024.107549","DOIUrl":"10.1016/j.anireprosci.2024.107549","url":null,"abstract":"<div><div>The recognition of animals as sentient beings has raised societal awareness of the welfare of farmed animals. This has been instrumental in increasing the legislative pressure towards welfare-conscious farming practices and, more lately, greater consumer demand for ethically sourced products. Besides, improved animal welfare is a key driver for enhanced performance, particularly in breeding boars, as welfare conditions strongly influence reproductive outcomes. The stressors associated with confinement impact testicular physiology and semen quality and the efforts to improve the welfare of breeding boars have so far focused on mitigating the negative consequences associated with stressors. The Five Freedoms framework and the European Food Safety Authority (EFSA)’s ‘welfare consequences’ approach prioritise the alleviation of suffering. In breeding boars, key welfare negative consequences include movement restriction, isolation stress, inability to engage in exploratory behaviours, locomotory disorders, and prolonged hunger. The negative consequences, which can be mitigated by improved housing and management practices, should be tackled in any commitment to improve the welfare of breeding boars. Animal welfare science, however, has recently shifted towards cultivating positive welfare experiences and a life worth living, beyond just alleviating suffering. The Five Domain Model systematically evaluates animal welfare, considering both negative and positive aspects. Encouraging positive welfare states involves facilitating species-specific behaviours, such as exploration and play, and fostering positive human-animal interactions. In breeding boars, strategies promoting positive welfare include providing enriching environments, encouraging exploration, and cultivating positive interactions with caretakers. Thus, for an overall welfare improvement of breeding boars, not only should the absence of suffering be guaranteed, but also the promotion of positive experiences that make their lifes worth living.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"269 ","pages":"Article 107549"},"PeriodicalIF":2.2,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141632422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunological defense mechanisms of ejaculates and the spread of viral infectious diseases through pig semen 射精的免疫防御机制和病毒性传染病通过猪精液的传播。

IF 2.2 2区农林科学

Animal Reproduction Science Pub Date : 2024-10-01 DOI: 10.1016/j.anireprosci.2024.107535

{"title":"Immunological defense mechanisms of ejaculates and the spread of viral infectious diseases through pig semen","authors":"","doi":"10.1016/j.anireprosci.2024.107535","DOIUrl":"10.1016/j.anireprosci.2024.107535","url":null,"abstract":"<div><div><span>This review focuses on the mechanisms of immune tolerance and antimicrobial defense in the male genital tract of the pig<span>. Sperm cells are foreign to the immune system and, therefore, they must be protected from the immune system. The blood-testis-barrier is mediated by a physical barrier between adjacent </span></span>Sertoli cells<span>, several cell types within the testis<span>, and interactions between immunomodulatory molecules. The blood-epididymal-barrier is composed of a physical barrier that is lined with principal cells having a network of junctional complexes in their apical lateral membrane and completed by specific transporters. The seminal plasma (SP) contains many signaling agents involved in establishing a state of immune tolerance in the female genital tract, which is essential for successful fertilization. Specific SP-proteins, however, also have pro-inflammatory capacities contributing to transient uterine inflammation, supporting the removal of foreign cells, possible pathogens, and excessive spermatozoa. While many different proteins and other substances present in semen can damage sperm cells, they may also protect them against viral infections. A delicate balance of these substances, therefore, needs to be maintained. Related to this, recent studies have shown the importance of extracellular vesicles (EVs), as they contain these substances and convey immune signals. Yet, viruses may use EVs to interact with the male genital tract and circumvent immune responses. For this reason, further research needs to explore the role of EVs in the male reproductive tract, as it might contribute to elucidating the pathogenesis of viral infections that might be transmitted via semen and to developing better vaccines.</span></span></div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"269 ","pages":"Article 107535"},"PeriodicalIF":2.2,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141330318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Artificial intelligence and porcine breeding 人工智能与猪育种

IF 2.2 2区农林科学

Animal Reproduction Science Pub Date : 2024-10-01 DOI: 10.1016/j.anireprosci.2024.107538

{"title":"Artificial intelligence and porcine breeding","authors":"","doi":"10.1016/j.anireprosci.2024.107538","DOIUrl":"10.1016/j.anireprosci.2024.107538","url":null,"abstract":"<div><div>Livestock management is evolving into a new era, characterized by the analysis of vast quantities of data (Big Data) collected from both traditional breeding methods and new technologies such as sensors, automated monitoring system, and advanced analytics. Artificial intelligence (A-In), which refers to the capability of machines to mimic human intelligence, including subfields like machine learning and deep learning, is playing a pivotal role in this transformation. A wide array of A-In techniques, successfully employed in various industrial and scientific contexts, are now being integrated into mainstream livestock management practices. In the case of swine breeding, while traditional methods have yielded considerable success, the increasing amount of information requires the adoption of new technologies such as A-In to drive productivity, enhance animal welfare, and reduce environmental impact. Current findings suggest that these techniques have the potential to match or exceed the performance of traditional methods, often being more scalable in terms of efficiency and sustainability within the breeding industry. This review provides insights into the application of A-In in porcine breeding, from the perspectives of both sows (including welfare and reproductive management) and boars (including semen quality and health), and explores new approaches which are already being applied in other species.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"269 ","pages":"Article 107538"},"PeriodicalIF":2.2,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141454696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimal conditions for cryopreservation by vitrification of largemouth bass (Micropterus salmoides) embryos 大口鲈鱼（Micropterus salmoides）胚胎玻璃化冷冻保存的最佳条件

IF 2.2 2区农林科学

Animal Reproduction Science Pub Date : 2024-09-27 DOI: 10.1016/j.anireprosci.2024.107613

Meng Zhang , Xiao Ma , Yuqing Han , Zerui Wang , Zhilin Jia , Dongcai Chen , Zhigang Qiao , Xiaotian Gao , Chunlong Zhao , Yawei Shen

{"title":"Optimal conditions for cryopreservation by vitrification of largemouth bass (Micropterus salmoides) embryos","authors":"Meng Zhang , Xiao Ma , Yuqing Han , Zerui Wang , Zhilin Jia , Dongcai Chen , Zhigang Qiao , Xiaotian Gao , Chunlong Zhao , Yawei Shen","doi":"10.1016/j.anireprosci.2024.107613","DOIUrl":"10.1016/j.anireprosci.2024.107613","url":null,"abstract":"<div><div>The largemouth bass (<em>Micropterus salmoides</em>) is one of the important freshwater aquaculture species in the world. However, due to limitations on introduction scale, high-density farming, inbreeding, and species hybridization, the germplasm resources of largemouth bass face threats such as degradation and susceptibility to diseases. Therefore, it is urgent to conduct research on the conservation of its original and good germplasm resources. We optimized the conditions of cryopreservation to vitrify and revive largemouth bass embryos, including the mixing ratio of cryoprotectants, embryo stage, equilibration step and temperature, and washing regent. The results showed that the least toxic single, binary, and ternary mixed permeating cryoprotectants were PG, PM (PG: MeOH = 2:1), and PMD (PM: DMSO = 3:1), respectively. The least toxic non-permeating cryoprotectant was 5 % glucose. The optimal vitrification solution selected was PMDG (30 % PMD + 5 % glucose) with an 80.67 % survival rate of embryos. Embryos at the heartbeat stage exhibited strong tolerance to the PMDG solution, which is the optimal embryo stage for cryopreservation. During the equilibration process, either the five-step equilibration method or pre-cooling the cryoprotectant to 4°C could reduce its toxicity. During the washing process, a 0.125 mol·L<sup>−1</sup> sucrose solution yielded the best results. Based on the optimized conditions, 650 embryos at the heartbeat stage were subjected to cryopreservation by vitrification, resulting in a total of 350 intact transparent eggs, two of which hatched successfully. The results provide a reference for further improving the efficiency of cryopreservation by vitrification of largemouth bass and other fish species.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"270 ","pages":"Article 107613"},"PeriodicalIF":2.2,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142327038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Folic acids promote in vitro maturation of bovine oocytes by inhibition of ferroptosis via upregulated glutathione and downregulated Fe2+ accumulation 叶酸通过上调谷胱甘肽和下调Fe2+积累来抑制铁突变，从而促进牛卵母细胞的体外成熟。

IF 2.2 2区农林科学

Animal Reproduction Science Pub Date : 2024-09-27 DOI: 10.1016/j.anireprosci.2024.107605

Zhuo Yang , Yaochang Wei , Yu Fu , Xiaoyan Wang , Wenjuan Shen , An Shi , Han Zhang , Heqiang Li , Xuexiao Song , Jie Wang , Mengdong Jin , Hao Zheng , Jinzhong Tao , Yongsheng Wang

{"title":"Folic acids promote in vitro maturation of bovine oocytes by inhibition of ferroptosis via upregulated glutathione and downregulated Fe2+ accumulation","authors":"Zhuo Yang , Yaochang Wei , Yu Fu , Xiaoyan Wang , Wenjuan Shen , An Shi , Han Zhang , Heqiang Li , Xuexiao Song , Jie Wang , Mengdong Jin , Hao Zheng , Jinzhong Tao , Yongsheng Wang","doi":"10.1016/j.anireprosci.2024.107605","DOIUrl":"10.1016/j.anireprosci.2024.107605","url":null,"abstract":"<div><div>Bovine embryos by <em>in vitro</em> fertilization have become the primary source of commercial embryo transfers globally. However, the developmental capacity of <em>in vitro</em> maturation (IVM) oocytes is considerably lower than that of <em>in vivo</em> maturation (IVO) oocytes, owing to the production of reactive oxygen species (ROS) via mitochondrial metabolism, which was higher in IVM oocytes than in IVO oocytes. To avoid the negative effects of ROS on embryo quality, folic acid (FA) was supplemented directly into the IVM medium to antagonize ROS production, however, the mechanisms remain unknown. In the present study, five levels of FA (0, 25, 50, 100, and 200 µM) were supplemented into the bovine oocyte culture medium. The maturation, cleavage, and blastocyst formation rates increased by 8.95 %, 6.94 %, and 4.36 %, respectively, in the 50 µM group compared to the 0 µM group. Moreover, 7904 differential genes were identified between 0 µM and 50 µM groups by transcriptome sequencing, and they were mainly enriched in 8 pathways. The glutathione, ROS, and Fe<sup>2+</sup> levels in oocytes were found to be associated with ferroptosis. Our results revealed that 50 µM FA promoted the IVM of bovine oocytes and affected the expression of genes involved in the ferroptosis pathway. The downregulation of <em>TFR1</em> and <em>STEAP3</em> led to a decrease in intracellular Fe<sup>2+</sup> accumulation, and the upregulation of <em>GCL</em> increased oocyte GSH levels, thereby reducing the production of ROS in the ferroptosis pathway. Our study provides a new insight into the molecular mechanisms by which FA promotes bovine oocyte development <em>in vitro</em>.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"270 ","pages":"Article 107605"},"PeriodicalIF":2.2,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142370787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibition of testicular development by suppressing neonatal LH rise in male domestic pigs 通过抑制雄性家猪新生儿 LH 的升高来抑制睾丸的发育。

IF 2.2 2区农林科学

Animal Reproduction Science Pub Date : 2024-09-27 DOI: 10.1016/j.anireprosci.2024.107606

ChanJin Park , Sandra Soto-Heras , Lindsey Reinacher , Katie Chai , Sherry Zhou , Po-Ching Lin , Ji-Eun Oh , Mary Bunnell , Rex A. Hess , Luiz Renato de França , CheMyong Ko

{"title":"Inhibition of testicular development by suppressing neonatal LH rise in male domestic pigs","authors":"ChanJin Park , Sandra Soto-Heras , Lindsey Reinacher , Katie Chai , Sherry Zhou , Po-Ching Lin , Ji-Eun Oh , Mary Bunnell , Rex A. Hess , Luiz Renato de França , CheMyong Ko","doi":"10.1016/j.anireprosci.2024.107606","DOIUrl":"10.1016/j.anireprosci.2024.107606","url":null,"abstract":"<div><div>The neonatal increase in circulating luteinizing hormone (LH) is crucial for testicular development. In male pigs, blood LH levels start to increase approximately 1 week after birth and return to basal level by 5–6 weeks of age. This study tested the hypothesis that neonatal treatment with a combination of estrogens and androgens suppresses LH secretion and thereby inhibits testicular development. On Day 1 after birth, piglets received a slow-release implant containing estradiol (E<sub>2</sub>, 8–40 mg) and trenbolone acetate (TBA, 40–200 mg) or remained intact. At 4 weeks of age, mean serum LH concentrations were ∼ 7 ng/mL in untreated males, whereas pigs with implants had serum LH concentrations < 1 ng/mL. Despite this reduction, LH was still detected in the pituitary glands of treated pigs. Interestingly, neonatal castration also lowered circulating LH, highlighting the importance of testis physiology in the early establishment of the reproductive axis. The higher dose (20 mg E2 + 100 mg TBA) inhibited testis function more effectively, as evidenced by lower circulating testosterone concentrations compared to intact pigs. Furthermore, E2 + TBA treatment had a lasting impact on testicular growth, resulting in smaller testes at 26 weeks of age and the presence of immature Leydig cells. Overall, neonatal E2 + TBA treatment suppressed the postnatal LH rise and testicular growth until market age, offering a potential non-surgical alternative to castration in male pigs.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"270 ","pages":"Article 107606"},"PeriodicalIF":2.2,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142493069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quality assurance applied to semen analysis 将质量保证应用于精液分析。

IF 2.2 2区农林科学

Animal Reproduction Science Pub Date : 2024-09-27 DOI: 10.1016/j.anireprosci.2024.107615

Leonardo F.C. Brito

引用次数: 0

MiR-29b inhibits COC expansion and oocyte in vitro maturation via induction of ROS by targeting CYCS MiR-29b 通过靶向 CYCS 诱导 ROS 来抑制 COC 扩增和卵母细胞体外成熟。

IF 2.2 2区农林科学

Animal Reproduction Science Pub Date : 2024-09-19 DOI: 10.1016/j.anireprosci.2024.107598

Xiaoshu Zhan , Nanshan Qi , Derek Toms , Renee Freiburger , Lauren Fletcher , Bingyun Wang , Julang Li

{"title":"MiR-29b inhibits COC expansion and oocyte in vitro maturation via induction of ROS by targeting CYCS","authors":"Xiaoshu Zhan , Nanshan Qi , Derek Toms , Renee Freiburger , Lauren Fletcher , Bingyun Wang , Julang Li","doi":"10.1016/j.anireprosci.2024.107598","DOIUrl":"10.1016/j.anireprosci.2024.107598","url":null,"abstract":"<div><div>Cumulus-oocyte complex (COC) expansion and oocyte maturation are crucial processes for embryo development and fertility across species. Although miR-29b has been detected in porcine ovarian granulosa cells, its specific role in regulating oocyte maturation remains largely unknown. In this study, using the pig as a model, we report that over-expression of miR-29b lead to a decrease of COC expansion area and inhibits oocyte maturation (<em>P</em><0.05). This suppression correlated with a decrease expression of COC-expansion-associated genes, including <em>SHAS2</em>, <em>ADAMTS1</em>, <em>ADAMTS2</em>, <em>ADAMTS17</em> and <em>PTX 3</em> in both mural granulosa cells (mGCs) and cumulus granulosa cells (cGCs). Further investigation revealed that miR-29b over-expression induces reactive oxygen species (ROS) accumulation in both mGCs and cGCs, conversely, knock-down of miR-29b reverses all these effects. Treatment with the antioxidant β-mercaptoethanol alleviates ROS accumulation, rescues COC expansion and restores oocyte polar body formation impaired by miR-29b mimics. Computational analysis predicted <em>CYCS</em>, the gene encoding cytochrome C, as a potential target of miR-29b. Subsequent examination demonstrated that miR-29b downregulates CYCS at both mRNA and protein levels. Dual-luciferase reporter assays further confirmed that miR-29b interacts with the 3’-untranslated region (3’UTR) of CYCS. Over-expression of CYCS decreases ROS accumulation and promotes COC expansion (<em>P</em><0.05). These results indicate that miR-29b regulates COC expansion and oocyte maturation <em>in vitro</em> by inducing ROS, likely through targeting of CYCS. This study sheds light on the role of miR-29b in oocyte maturation and provides insight into the regulatory function of miRNAs in ovarian physiology.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"270 ","pages":"Article 107598"},"PeriodicalIF":2.2,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142339537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0