Animal Reproduction Science最新文献

{"title":"Omics approaches to understand impact of heat stress on semen quality and fertility in bovines","authors":"Meenakshi Chitkara ,&nbsp;Ankita Gurao ,&nbsp;Harsimran Kaur ,&nbsp;Mahesh Shivanand Dige ,&nbsp;Monika Sodhi ,&nbsp;Manishi Mukesh ,&nbsp;Ranjit Singh Kataria","doi":"10.1016/j.anireprosci.2025.107786","DOIUrl":"10.1016/j.anireprosci.2025.107786","url":null,"abstract":"<div><div>Recent surge in global climate change poses unprecedented challenges to traditional livestock breeding methods. In tropical countries, where large bovines are vital source of meat and milk, the escalating global warming significantly impact their productivity. Central to this challenge is the resilient ability of the animals, which directly influences the productivity. Artificial insemination (AI) programs, a cornerstone in modern livestock production, have also been severely hampered by climate change-induced heat stress, affecting the quality semen production. This stress not only affects the blood biochemical profiles of the animals but also their testicular physiology, leading to the issues such as low-quality semen with compromised freezability and fertility. Researchers have identified specific markers, including single nucleotide polymorphisms (SNPs), copy number variations (CNV), and epigenetic signatures like histone modifications, DNA methylation, and noncoding RNAs, influencing the semen quality in livestock species. Furthermore, the issue of heat stress has been addressed in a very precise way, and biomarkers have been identified, which can be integrated into the breeding programme to keep up the sire summary. Transcriptomic studies have further illuminated the temporal expression patterns of genes related to sperm quality during heat stress, pinpointing candidate genes for further exploration. This review comprehensively summarizes the progress made in understanding the intricacies of sperm biology in bovines, with a specific focus on cattle and buffalo delving into a spectrum of changes, from biochemical shifts to profound cellular alterations, including genomic, transcriptomic, and epigenetic modifications.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"274 ","pages":"Article 107786"},"PeriodicalIF":2.2,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143195069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of a practical method for short-term stored common carp sperm with extenders and quality adjustment before fertilization for breeding success","authors":"Nururshopa Eskander Shazada ,&nbsp;Mohammad Abdul Momin Siddique ,&nbsp;Songpei Zhang ,&nbsp;Zhijun Ma ,&nbsp;Marek Rodina ,&nbsp;Zuzana Linhartová ,&nbsp;Otomar Linhart","doi":"10.1016/j.anireprosci.2025.107763","DOIUrl":"10.1016/j.anireprosci.2025.107763","url":null,"abstract":"<div><div>The main objective of this study was to elucidate whether there are any effects of sperm dilution time before short-term storage in common carp. The sperm of five males (2–2.5 kg body weight, 3 years old) with good spermatozoa motility (˃90 %) were diluted with carp extender immediately, 3 h post-diluted, and undiluted fresh sperm was used as control. Sperm motility and velocity of undiluted and diluted sperm were evaluated using a CASA system. A two-way ANOVA revealed that spermatozoa motility, curvilinear velocity (VCL), and straight-line velocity (VSL) were significantly impacted by storage time, varying dilution with extenders, and their interaction (p &lt; 0.001). Sperm motility and velocity dropped steadily with increasing storage periods for diluted sperm, while they drastically fell for undiluted sperm and became immotile at 5 days post-storage. In the control group, the mean percentage of sperm motility was achieved by 40.60 % at 3 days post-storage and sperm diluted with Cejko solution immediately and 3 h post-diluted sperm retained motility at 65.13 % and 65.63 %, respectively, at 3 days post-storage and retained motility of 39.45 and 40.43 %, respectively, at 7 days post-storage. After 7 days post-storage, the diluted (immediately and 3 h post-diluted) pooled sperm had a 61.01 % and 56.70 % fertilization and a 43.40 % and 36.52 % hatching rate, whereas fresh sperm had an 89.34 % fertilization and 78.06 % hatching success. According to the results of the fertilization and hatching assay, compared to fresh sperm, 7 days of stored diluted pooled sperm produced a 68.29 % fertilization rate and a 55.60 % hatching rate.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"274 ","pages":"Article 107763"},"PeriodicalIF":2.2,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143377089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IGF-1 outperforms vitamin E and crocin in improving semen quality, antioxidant profile and fertility gene expression in cryopreserved goat (Capra hircus) semen","authors":"W. Lomen Singh ,&nbsp;Sudip Sinha ,&nbsp;Kutubuddin Ahmed ,&nbsp;Lukumoni Buragohain ,&nbsp;Mohua Das Gupta ,&nbsp;Dipak Bhuyan ,&nbsp;Shantanu Tamuly ,&nbsp;Sayed Nabil Abedin ,&nbsp;Souvik Dhara ,&nbsp;Maradona Nath","doi":"10.1016/j.anireprosci.2025.107791","DOIUrl":"10.1016/j.anireprosci.2025.107791","url":null,"abstract":"<div><div>Semen cryopreservation combined with artificial insemination (AI) benefits germplasm conservation, production, and breeding. Sperm cells are vulnerable to oxidative and other stressors during cryopreservation, necessitating the use of additives/antioxidants to mitigate this damage. This study aimed to evaluate the effects of vitamin E, IGF-1, and crocin supplementation on the quality of cryopreserved goat semen. A total of 48 pooled ejaculates from six goat bucks were diluted in Tris-Citrate-Fructose-Egg Yolk-Glycerol (TCFEYG) extender and divided into four groups: a control group (no antioxidant addition) and groups supplemented with 2 mM vitamin E, 125 ng/mL IGF-1 and 1 mM crocin. The semen samples were cryopreserved in liquid nitrogen (LN₂) and analyzed post-thaw for sperm quality attributes, antioxidant enzyme activities and mRNA expression levels. IGF-1 supplementation significantly (<em>P</em> &lt; 0.05) enhanced <em>in vitro</em> sperm quality, and antioxidant enzyme <em>viz</em>., catalase (CAT), glutathione reductase (GR), glutathione peroxidase (GPx), glutathione S transferase (GST) and superoxide dismutase (SOD) compared to other treatments. Lipid peroxidation (LPO) levels were significantly (<em>P</em> &lt; 0.05) lower, and fertility-related mRNA transcripts (<em>NFE2L2, GPx4, CAT, and SOD2</em>) were significantly (<em>P</em> &lt; 0.05) upregulated in the IGF-1 group. No significant (<em>P</em> &gt; 0.05) differences in pregnancy rates were observed among treatments after AI. In conclusion, IGF-1 supplementation significantly improved the post-thaw quality of cryopreserved goat semen, enhancing antioxidant activity and fertility-related mRNA expression compared to vitamin E and crocin treatments.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"274 ","pages":"Article 107791"},"PeriodicalIF":2.2,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143078313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating sperm characteristics from stripping and testicular extraction in northern pike (Esox lucius) with a focus on stability during storage","authors":"Beata Irena Cejko ,&nbsp;Sławomir Krejszeff ,&nbsp;Agata Anna Cejko ,&nbsp;Katarzyna Dryl","doi":"10.1016/j.anireprosci.2025.107789","DOIUrl":"10.1016/j.anireprosci.2025.107789","url":null,"abstract":"<div><div>The quality and quantity of stripped and testicular wild northern pike (<em>Esox lucius</em>) sperm was compared and the effectiveness of short-term storage was assessed. Stripped sperm (SS) was collected using abdominal massage. Next, the fish were decapitated and the testes were removed. Testicular sperm (TS) was collected by gently macerating the testes through double-layered gauze. The volume and number of sperm were assessed in SS and TS. Computer-assisted sperm analysis (CASA) was used to determine SS and TS motility and kinetic parameters. Undiluted samples of SS and TS were then stored short-term under refrigeration (8°C) for 72 h. A higher volume and number of sperm were noted for TS compared to SS. On the other hand, motility and kinetic parameters of SS and TS were similar immediately after collection. Seminal plasma osmotic pressure was lower in SS compared to TS. Regardless of the sperm source (SS or TS), values of sperm motility and kinetic parameters remained high during short-term storage for 24 h. However, after 48 and 72 h significant decreases in the values of MOT, curvilinear velocity (VCL, μm s⁻¹), straight-linear velocity (VSL, µm s⁻¹), and amplitude of lateral head displacement (ALH, µm) parameters were noted in SS compared to TS. This indicated that northern pike sperm obtained from spermatic ducts lost their biological value (fertilization capacity) much faster than that obtained from testes. This was most likely due to the presence of urine and its long-term negative impact on storage efficiency of SS compared to TS.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"274 ","pages":"Article 107789"},"PeriodicalIF":2.2,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143063357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Incorporating olive (Olea europaea L) fruit extracts in a tris-based extender improves buffalo semen cryotolerance by reducing oxidative stress","authors":"Maria Paz Benitez Mora ,&nbsp;Michal Andrzej Kosior ,&nbsp;Valentina Longobardi ,&nbsp;Chiara Del Prete ,&nbsp;Francesca Luisa Fedele ,&nbsp;Alessia Staropoli ,&nbsp;Giulio Guido Aiudi ,&nbsp;Natascia Cocchia ,&nbsp;Bianca Gasparrini","doi":"10.1016/j.anireprosci.2025.107787","DOIUrl":"10.1016/j.anireprosci.2025.107787","url":null,"abstract":"<div><div>This work aimed to evaluate whether supplementing the freezing extender with olive fruit extract (OFE) would improve the antioxidant defense of buffalo sperm, resulting in improved post-thaw semen quality. Ejaculates (two per 16 Murrah buffalo bulls) were split into four aliquots that were diluted in an extender supplemented with different doses of OFE (0, D50, D100, and D150, based on µM concentrations of hydroxytyrosol, the most represented polyphenol) and frozen according to standard procedures. At thawing, sperm motility, kinetics, viability, acrosome integrity, and membrane functionality were evaluated. Based on the dose-response results, biological antioxidant potential (BAP) and reactive oxygen metabolites (ROMs) were assessed after thawing in D50 and control groups. The pre-freezing supplementation of the extender with D50 OFE showed higher (<em>P</em> &lt; 0.05) total and progressive sperm motility, as well as straight-line velocity compared to the control. Treatment with D50 OFE of buffalo semen also improved (<em>P</em> &lt; 0.01) post-thaw sperm viability, membrane functionality, and acrosome integrity compared to the control. The enrichment of the extender with D50 OFE increased (<em>P</em> &lt; 0.01) the post-thaw BAP and reduced (<em>P</em> &lt; 0.05) the ROMs levels. The highest concentration tested (D150 OFE) negatively affected (<em>P</em> &lt; 0.05) total and progressive motility, and the percentage of sperm with functional membranes and intact acrosomes, compared to the control. In conclusion, low doses of OFE added to the extender significantly improved post-thawing buffalo semen quality by protecting the spermatozoa from cryopreservation-induced oxidative stress. Further studies should investigate its effectiveness on <em>in vivo</em> and <em>in vitro</em> fertility, for potential commercial applications.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"274 ","pages":"Article 107787"},"PeriodicalIF":2.2,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143063359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of animal temperament on body and reproductive development of beef bulls","authors":"Mariana Karla Francolino da Silva ,&nbsp;Marcilio Nichi ,&nbsp;Marcelo Sant’Ana Borges ,&nbsp;Richardt Gama Landgraf ,&nbsp;Marcelo Francisco Arantes Pereira ,&nbsp;André Maciel Crespilho","doi":"10.1016/j.anireprosci.2025.107788","DOIUrl":"10.1016/j.anireprosci.2025.107788","url":null,"abstract":"<div><div>Several studies have related reactive temperament to poorer productive and reproductive performance in cattle. However, no studies have aimed to investigate the effect of temperament on sexual development and precocity of male cattle. The objective of this study was to test the hypothesis that reactive animals exhibit delayed sexual development and poorer reproductive performance. For the study, 40 crossbred male cattle (<em>Nellore</em> x <em>Santa Gertrudis</em>) at 12 months were selected. The animals underwent behavioral evaluation and were divided into two groups: calm (CA; <em>n</em> = 24) and reactive (RE; <em>n</em> = 16). All steers were weighed to monitor weight gain, and blood samples were collected to measure cortisol and testosterone concentrations. Semen collections by electroejaculation were performed to determine the onset of puberty and assess sperm quality. Cryopreservation tests were conducted, and the samples were evaluated for kinetics and plasma membrane integrity. Ultrasound examinations assessed testicular development. Additionally, testicular biopsies were performed to evaluate spermatid and spermatozoa ratios. There was a trend toward higher cortisol production (<em>P</em> = 0.07) in RE animals. Higher total (<em>P</em> = 0.03), average (<em>P</em> = 0.07), and daily (<em>P</em> = 0.06) weight gains were observed for CA animals. RE steers produced a higher proportion of cells with minor sperm defects (<em>P</em> = 0.06). Greater vesicular glands development was observed in CA animals (<em>P</em> = 0.01). No effect of temperament was observed on sexual precocity, cryotolerance, or testosterone production. It was concluded that although temperament does not influence age at puberty, reactive bulls exhibit poorer performance in body development, vesicular glands development, and fresh semen quality compared to calm bulls.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"274 ","pages":"Article 107788"},"PeriodicalIF":2.2,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143073179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro effect of omentin-1 on level of other adipokines in granulosa cells from ovaries of Large White and Meishan pigs","authors":"Karolina Pich ,&nbsp;Natalia Respekta-Długosz ,&nbsp;Monika Dawid ,&nbsp;Christelle Rame ,&nbsp;Nina Smolińska ,&nbsp;Joëlle Dupont ,&nbsp;Agnieszka Rak","doi":"10.1016/j.anireprosci.2025.107783","DOIUrl":"10.1016/j.anireprosci.2025.107783","url":null,"abstract":"<div><div>Omentin-1, also known as intelectin-1 (ITLN1), is an adipokine produced by visceral adipose tissue and effect on improve insulin sensitivity, regulate energy metabolism and reproduction. Our previously data has shown that ITLN1 expression in porcine ovarian follicles is influenced by both the level of adiposity and the phase of the estrous cycle. Additionally, recent studies have indicated variations in the serum levels of adipokines in pigs with higher fat content, suggest their common interaction. It was therefore hypothesized that ITLN1 regulates the levels of other adipokines and their receptors in the ovary of pigs with different fat content. The aim of this study was to investigate the <em>in vitro</em> effect of ITLN1 (10–100 ng/mL) on the levels of other adipokines, namely adiponectin, apelin, leptin, chemerin, resistin, visfatin, and vaspin in the culture medium, assessed by ELISA. In addition, the effect of ITLN1 on the mRNA expression of the above-mentioned adipokines with their receptors was investigated using RT-qPCR in granulosa cells (Gc) from normal weight Large White (LW) and fat Meishan (MS) pigs on days 10–12 of the estrous cycle. The results showed that ITLN1 reduced apelin levels, increased leptin, and had no effect on vaspin in the culture medium. ITLN1 was found to have a modulating effect on the mRNA expression of the other adipokines with their receptors in porcine Gc. It can, therefore, be proposed that ITLN1 represents a previously unidentified auto- and paracrine regulator of the functions of ovarian microenvironmental compartments in different types of pig breeds.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"274 ","pages":"Article 107783"},"PeriodicalIF":2.2,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143073178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Superovulation treatment of immature female rabbits increases the number of ovulated oocytes that can in vitro develop into blastocytes","authors":"Toshiaki Akiyoshi ,&nbsp;Fumikazu Matsuhisa ,&nbsp;Masashi Shiomi ,&nbsp;Masatoshi Morimoto ,&nbsp;Shuji Kitajima","doi":"10.1016/j.anireprosci.2025.107765","DOIUrl":"10.1016/j.anireprosci.2025.107765","url":null,"abstract":"<div><div>To clarify the efficiency of superovulation in immature female rabbits, immature female rabbits were superovulated with equine chorionic gonadotropin, and the number of recovered oocytes, their maturity, and their ability to develop into blastocysts under <em>in vitro</em> fertilization and culture were examined in this study. More than 80 oocytes were recovered from 12–14-week-old immature female rabbits. In particular, an average of more than 100 oocytes were recovered from 13-week-old immature female rabbits. The number of oocytes in immature female rabbits was significantly-approximately 4 times-higher than in mature female rabbits. To compare oocyte maturity, oocytes from immature and mature female rabbits were compared by brilliant cresyl blue staining and mitochondrial distribution analysis. The proportion of mature oocytes detected by brilliant cresyl blue staining was higher in oocytes from mature female rabbits than in oocytes from immature female rabbits, but there were no significant differences in active mitochondrial distribution, fertilization rate, or blastocyst development rate. These results indicate that superovulation with immature female rabbits may be a useful technique for the collection of many oocytes.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"274 ","pages":"Article 107765"},"PeriodicalIF":2.2,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142998806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of luteal blood perfusion and expression of estrus on pregnancy rates of Bos taurus embryo recipients exposed to fixed-time embryo transfer","authors":"Lucas Melo Goncalves ,&nbsp;Samir Burato ,&nbsp;Madison Blake Walker ,&nbsp;Molly Smith ,&nbsp;Lucas Neira ,&nbsp;Gregory Clements ,&nbsp;Shane Morgan ,&nbsp;Reinaldo Fernandes Cooke ,&nbsp;Pedro Levy Piza Fontes","doi":"10.1016/j.anireprosci.2025.107764","DOIUrl":"10.1016/j.anireprosci.2025.107764","url":null,"abstract":"<div><div>This study evaluated the impact of luteal blood perfusion (BP) and expression of estrus on pregnancy rates of <em>Bos taurus</em> embryo recipients exposed to fixed-timed embryo transfer (FTET) using a gonadotropin-releasing hormone (GnRH)- and progesterone-based ovulation synchronization protocol. Postpartum beef cows (<em>n</em> = 746) were exposed to a GnRH/progesterone-based ovulation synchronization protocol. Luteal morphometry and BP were assessed using color Doppler ultrasonography 9 days after CIDR removal concurrently with FTET. Embryo recipients were retrospectively categorized according to CL area [small (&lt; 2.5 cm²), medium (2.5–3.5 cm²), or large (&gt; 3.5 cm²)] and BP [low (≤ 30 %), medium (&gt;30 and ≤ 40 %), or high (&gt; 40 % of luteal area with BP signals)]. Luteal area classification did not impact pregnancy rates (<em>P</em> = 0.50); however, cows with high luteal B<em>P</em> had greater (<em>P</em> &lt; 0.01) pregnancy rates compared with low BP cows and tended to have greater pregnancy rates compared with medium BP cows (<em>P</em> = 0.07). Embryo recipients that expressed behavioral estrus had larger (<em>P</em> &lt; 0.01) and more vascularized CL (<em>P</em> = 0.02), as well as greater (<em>P</em> = 0.02) pregnancy rates compared with females that did not express estrus. Nevertheless, no interaction between expression of behavioral estrus and luteal BP classification was observed (<em>P</em> = 0.41). In summary, luteal BP at FTET and expression of behavioral estrus were positively associated with pregnancy rates in <em>Bos taurus</em> embryo recipients exposed to progesterone- and GnRH-based protocols.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"274 ","pages":"Article 107764"},"PeriodicalIF":2.2,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142998805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relative increase in walking activity in lactating dairy cows submitted to timed artificial insemination: Risk factors and subsequent fertility","authors":"E. Rojas Canadas,&nbsp;I. García-Ispierto","doi":"10.1016/j.anireprosci.2025.107762","DOIUrl":"10.1016/j.anireprosci.2025.107762","url":null,"abstract":"<div><div>The objectives of this study were: (i) to evaluate the associations between postpartum health disorders, relative increase in walking activity (RIWA) and expression of behavioral estrus intensity captured by pedometers; (ii) to assess associations between RIWA and pregnancy in cows submitted to timed artificial insemination (TAI). Lactating dairy cows (<em>n</em> = 881) were enrolled in the study. Cows were submitted to a 5d TAI protocol with insertion of a progesterone device. Pregnancy status was determined by ultrasonography on D30 after AI. During the postpartum period cows were categorized as healthy (<em>n</em> = 468), having a reproductive disorder (<em>n</em> = 185), metabolic disorder (<em>n</em> = 118), reproductive and metabolic disorders (n = 56), or clinical mastitis (<em>n</em> = 54). Healthy cows tended to have (<em>P</em> = 0.10) a greater RIWA and a lower proportion of healthy cows did not exhibit estrus (<em>P</em> = 0.03) compared with cows that had a postpartum health disorder. Similarly, ovarian status at TAI protocol commencement (<em>P</em> = 0.01), presence of twin pregnancy (<em>P</em> = 0.03), and season (<em>P</em> &lt; 0.0001) were all associated with RIWA. Cows with a corpus luteum (CL) at TAI protocol commencement, carrying twins and those served during the cold season had an enhanced RIWA compared with those with no CL, carrying singletons and cows served during the hot season. In addition, healthy cows (<em>P</em> = 0.003) and cows showing estrus (<em>P</em> = 0.0002) had enhanced pregnancy per TAI compared with those with postpartum disorders, and those failing to exhibit estrus, respectively. Hence, cows that remained healthy during the postpartum had a greater RIWA, expression of behavioral estrus intensity and pregnancy to TAI compared with those that had a postpartum disorder.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"274 ","pages":"Article 107762"},"PeriodicalIF":2.2,"publicationDate":"2025-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142969440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}