Relaxin-like gonad-stimulating peptide promotes gamete maturation and spawning in the sea cucumber Apostichopus japonicus

IF 2.2 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science Pub Date : 2025-03-05 DOI:10.1016/j.anireprosci.2025.107820

Jiaze Wang , Zhaoming Gao , Shuai Liu , Yongchao Hu , Libin Zhang , Beini Deng , Zhenxia Sha , Xiaoshang Ru

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Abstract

Apostichopus japonicus is a crucial aquaculture species in Asia, and seedling production is fundamental to its cultivation. Nevertheless, the reproductive behavior of this species remains difficult to regulate artificially. Although the spawning mode of sea cucumbers is well documented, the endocrine mechanisms that regulate the synchronized processes of oocyte maturation and spawning behavior remain poorly understood. In this study, the effects of a recombinant relaxin-like gonad-stimulating peptide (RGP) on oocyte maturation and parental spawning behavior were investigated. The recombinant RGP derived from A. japonicus was effectively expressed using the Pichia pastoris system, and its biological activity was validated through mass spectrometry analysis. Results indicated that the RGP promoted oocyte maturation by inducing the rupture of germinal vesicles. Behavioral studies revealed that the RGP enhanced gamete release. Furthermore, the gametes induced by the RGP did not differ significantly from those released spontaneously in terms of progeny quality and quantity. These findings suggested that the recombinant RGP could be utilized in the breeding of A. japonicus seedlings. They also had substantial implications for elucidating the reproductive behavior of this species and for the artificial induction of seedling production.

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来源期刊

Animal Reproduction Science 农林科学-奶制品与动物科学

CiteScore

4.50

自引率

9.10%

发文量

136

审稿时长

54 days

期刊介绍： Animal Reproduction Science publishes results from studies relating to reproduction and fertility in animals. This includes both fundamental research and applied studies, including management practices that increase our understanding of the biology and manipulation of reproduction. Manuscripts should go into depth in the mechanisms involved in the research reported, rather than a give a mere description of findings. The focus is on animals that are useful to humans including food- and fibre-producing; companion/recreational; captive; and endangered species including zoo animals, but excluding laboratory animals unless the results of the study provide new information that impacts the basic understanding of the biology or manipulation of reproduction. The journal''s scope includes the study of reproductive physiology and endocrinology, reproductive cycles, natural and artificial control of reproduction, preservation and use of gametes and embryos, pregnancy and parturition, infertility and sterility, diagnostic and therapeutic techniques. The Editorial Board of Animal Reproduction Science has decided not to publish papers in which there is an exclusive examination of the in vitro development of oocytes and embryos; however, there will be consideration of papers that include in vitro studies where the source of the oocytes and/or development of the embryos beyond the blastocyst stage is part of the experimental design.