The use of wheat grass juice as a promising functional feed additive for enhancing reproductive performance and larvae quality of sterlet (Acipenser ruthenus)

IF 2.2 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science Pub Date : 2025-02-15 DOI:10.1016/j.anireprosci.2025.107796

C.-A. Barbacariu , G. Dumitru , L. Dîrvariu , D.A. Şerban , I. Dincheva , E. Todirascu-Ciornea , M. Burducea

{"title":"The use of wheat grass juice as a promising functional feed additive for enhancing reproductive performance and larvae quality of sterlet (Acipenser ruthenus)","authors":"C.-A. Barbacariu , G. Dumitru , L. Dîrvariu , D.A. Şerban , I. Dincheva , E. Todirascu-Ciornea , M. Burducea","doi":"10.1016/j.anireprosci.2025.107796","DOIUrl":null,"url":null,"abstract":"<div><div>The optimization of broodstock nutrition remains a critical challenge in sturgeon aquaculture. This study investigated the effects of wheatgrass juice (WGJ) supplementation of sterlet (<em>Acipenser ruthenus</em>) broodstock on reproductive performance, larval quality, hematological profile and oxidative status. Female sterlets were randomly assigned to three groups: control diet (V1), WGJ-supplemented diet (V2, 4 % inclusion), both (V1 and V2) hormonally stimulated and saline solution (V3) without hormonal stimulation. WGJ's chemical composition revealed substantial levels of bioactive compounds, including chlorophyll (a: 0.936 ± 0.01, b: 0.329 ± 0.01 mg/mL fw), carotenoids (0.167 ± 0.01 mg/mL fw), proteins (1.205 ± 0.04 mg PSB/mL fw), flavonoids (7.381 ± 0.31 mg/mL fw), and polyphenols (2.963 ± 0.05 mg/mL fw). WGJ supplementation significantly enhanced reproductive parameters, with V2 showing higher egg production (0.23 ± 0.03 kg vs. 0.13 ± 0.01 kg) and gonadosomatic index (14.48 ± 1.73 vs. 8.05 ± 0.51) compared to V1. Larvae from WGJ-supplemented females exhibited significantly improved length (10.9 ± 0.3 cm vs. 9.8 ± 0.14 cm, p < 0.001) and weight parameters. Analysis of oxidative stress markers revealed tissue-specific modulation of antioxidant systems, with WGJ supplementation reducing malondialdehyde levels across tissues (muscle: 1.061 ± 0.075 vs. 1.888 ± 0.105 nM/mg protein, p < 0.0001) and enhancing antioxidant enzyme activities. Hematological parameters remained within physiological ranges, with reduced neutrophil counts in the WGJ group suggesting potential immunomodulatory effects. These findings establish WGJ as a promising functional feed additive for enhancing reproductive performance in sterlet aquaculture, while maintaining optimal physiological status through improved antioxidant defense mechanisms.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"274 ","pages":"Article 107796"},"PeriodicalIF":2.2000,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Animal Reproduction Science","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0378432025000351","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}

引用次数: 0

Abstract

The optimization of broodstock nutrition remains a critical challenge in sturgeon aquaculture. This study investigated the effects of wheatgrass juice (WGJ) supplementation of sterlet (Acipenser ruthenus) broodstock on reproductive performance, larval quality, hematological profile and oxidative status. Female sterlets were randomly assigned to three groups: control diet (V1), WGJ-supplemented diet (V2, 4 % inclusion), both (V1 and V2) hormonally stimulated and saline solution (V3) without hormonal stimulation. WGJ's chemical composition revealed substantial levels of bioactive compounds, including chlorophyll (a: 0.936 ± 0.01, b: 0.329 ± 0.01 mg/mL fw), carotenoids (0.167 ± 0.01 mg/mL fw), proteins (1.205 ± 0.04 mg PSB/mL fw), flavonoids (7.381 ± 0.31 mg/mL fw), and polyphenols (2.963 ± 0.05 mg/mL fw). WGJ supplementation significantly enhanced reproductive parameters, with V2 showing higher egg production (0.23 ± 0.03 kg vs. 0.13 ± 0.01 kg) and gonadosomatic index (14.48 ± 1.73 vs. 8.05 ± 0.51) compared to V1. Larvae from WGJ-supplemented females exhibited significantly improved length (10.9 ± 0.3 cm vs. 9.8 ± 0.14 cm, p < 0.001) and weight parameters. Analysis of oxidative stress markers revealed tissue-specific modulation of antioxidant systems, with WGJ supplementation reducing malondialdehyde levels across tissues (muscle: 1.061 ± 0.075 vs. 1.888 ± 0.105 nM/mg protein, p < 0.0001) and enhancing antioxidant enzyme activities. Hematological parameters remained within physiological ranges, with reduced neutrophil counts in the WGJ group suggesting potential immunomodulatory effects. These findings establish WGJ as a promising functional feed additive for enhancing reproductive performance in sterlet aquaculture, while maintaining optimal physiological status through improved antioxidant defense mechanisms.

查看原文本刊更多论文

小麦草汁作为一种有前景的功能性饲料添加剂，可提高小鲟的繁殖性能和幼虫品质。

鱼类营养的优化仍然是鲟鱼养殖面临的关键挑战。本试验研究了添加小麦草汁（WGJ）对小鲟（acpenser ruthenus）繁殖性能、幼虫品质、血液学特征和氧化状态的影响。将雌性幼鱼随机分为3组：对照饲粮（V1）、添加wgj的饲粮（V2，含4 %）、激素刺激组（V1和V2）和无激素刺激的生理盐水组（V3）。WGJ化学成分显示大量的生物活性化合物的水平,包括叶绿素( :0.936±0.01 ,b: 0.329±0.01  毫克/毫升弗兰克-威廉姆斯),类胡萝卜素(0.167 ±0.01  毫克/毫升fw)、蛋白质(1.205 ±0.04 公安局 毫克/毫升弗兰克-威廉姆斯),类黄酮(7.381 ±0.31  毫克/毫升弗兰克-威廉姆斯),和多酚(2.963 ±0.05  毫克/毫升fw)。WGJ补充显著增强生殖参数,V2显示更高的产蛋(0.23 ±  公斤0.03 vs 0.13 ±0.01  公斤)和gonadosomatic指数(14.48 ±  1.73和8.05±0.51 )相比,V1。添加wgj的雌性幼虫的体长（10.9 ± 0.3 cm vs. 9.8 ± 0.14 cm, p <； 0.001）和体重参数显著提高。氧化应激标志物分析揭示了抗氧化系统的组织特异性调节，添加WGJ可降低组织间丙二醛水平（肌肉：1.061 ± 0.075 vs. 1.888 ± 0.105 nM/mg蛋白，p <； 0.0001）并增强抗氧化酶活性。血液学参数保持在生理范围内，WGJ组中性粒细胞计数减少，提示潜在的免疫调节作用。这些结果表明，WGJ是一种很有前景的功能性饲料添加剂，可以通过改善抗氧化防御机制来提高小鱼养殖的繁殖性能，同时保持最佳的生理状态。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Animal Reproduction Science 农林科学-奶制品与动物科学

CiteScore

4.50

自引率

9.10%

发文量

136

审稿时长

54 days

期刊介绍： Animal Reproduction Science publishes results from studies relating to reproduction and fertility in animals. This includes both fundamental research and applied studies, including management practices that increase our understanding of the biology and manipulation of reproduction. Manuscripts should go into depth in the mechanisms involved in the research reported, rather than a give a mere description of findings. The focus is on animals that are useful to humans including food- and fibre-producing; companion/recreational; captive; and endangered species including zoo animals, but excluding laboratory animals unless the results of the study provide new information that impacts the basic understanding of the biology or manipulation of reproduction. The journal''s scope includes the study of reproductive physiology and endocrinology, reproductive cycles, natural and artificial control of reproduction, preservation and use of gametes and embryos, pregnancy and parturition, infertility and sterility, diagnostic and therapeutic techniques. The Editorial Board of Animal Reproduction Science has decided not to publish papers in which there is an exclusive examination of the in vitro development of oocytes and embryos; however, there will be consideration of papers that include in vitro studies where the source of the oocytes and/or development of the embryos beyond the blastocyst stage is part of the experimental design.