Effects of hCG administered 5 or 7 days after the onset of induced estrus on luteal morphology and function in seasonally anovular dairy goats

IF 2.2 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science Pub Date : 2025-03-04 DOI:10.1016/j.anireprosci.2025.107818

Juliana Nascimento Duarte Rodrigues , José Domingos Guimarães , Paulo Sergio Cerqueira Rangel , Maria Emilia Franco Oliveira , Felipe Zandonadi Brandão , Pawel Mieczyslaw Bartlewski , Jeferson Ferreira Fonseca

{"title":"Effects of hCG administered 5 or 7 days after the onset of induced estrus on luteal morphology and function in seasonally anovular dairy goats","authors":"Juliana Nascimento Duarte Rodrigues , José Domingos Guimarães , Paulo Sergio Cerqueira Rangel , Maria Emilia Franco Oliveira , Felipe Zandonadi Brandão , Pawel Mieczyslaw Bartlewski , Jeferson Ferreira Fonseca","doi":"10.1016/j.anireprosci.2025.107818","DOIUrl":null,"url":null,"abstract":"<div><div>The objective was to evaluate the effects of intramuscular (i.m.) human chorionic gonadotropin (hCG) administered on day 5 (D5) or day 7 (D7) after estrus (D0) on luteal structures (number, area, vascularization, and progesterone secretion) and pregnancy rates in estrus-induced dairy goats during the non-breeding season. Thirty-four Alpine goats received intravaginal sponges containing medroxyprogesterone acetate for 6 days and a single i.m. injection of 200 international units (IU) of equine chorionic gonadotropin (eCG) plus 37.5 micrograms (µg) of d-cloprostenol 24 hours before sponge removal. All exhibited estrus and were mated by fertile bucks. Goats were randomly assigned to hCG-5 (300 IU on D5; n = 12), hCG-7 (300 IU on D7; n = 11), or controls (n = 11). Transrectal ovarian ultrasonography and blood sampling were performed on D5, D7, D10, D13, D17, and D21, with pregnancy diagnosis on D60. hCG treatment did not affect pregnancy rates. Accessory corpora lutea (aCL) appeared in 66.6 % (hCG-5) and 90.9 % (hCG-7) (P > 0.05). Total luteal area in hCG-7 was greater (P < 0.05) than in controls from D10 to D21. The mean original corpora lutea (oCL) area in hCG-7 was largest (P < 0.05) on D10. High-velocity Doppler area (HVDA) of aCL was greater (P < 0.05) in hCG-5 compared with hCG-7 on D13. Plasma progesterone (P4) concentrations were higher (P < 0.05) in hCG-7 than in controls from D10 to D21. Although hCG administered on D5 or D7 transiently enhanced luteal function and vascularization, it did not improve pregnancy outcomes in seasonally anovular dairy goats.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"275 ","pages":"Article 107818"},"PeriodicalIF":2.2000,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Animal Reproduction Science","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0378432025000570","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}

引用次数: 0

Abstract

The objective was to evaluate the effects of intramuscular (i.m.) human chorionic gonadotropin (hCG) administered on day 5 (D5) or day 7 (D7) after estrus (D0) on luteal structures (number, area, vascularization, and progesterone secretion) and pregnancy rates in estrus-induced dairy goats during the non-breeding season. Thirty-four Alpine goats received intravaginal sponges containing medroxyprogesterone acetate for 6 days and a single i.m. injection of 200 international units (IU) of equine chorionic gonadotropin (eCG) plus 37.5 micrograms (µg) of d-cloprostenol 24 hours before sponge removal. All exhibited estrus and were mated by fertile bucks. Goats were randomly assigned to hCG-5 (300 IU on D5; n = 12), hCG-7 (300 IU on D7; n = 11), or controls (n = 11). Transrectal ovarian ultrasonography and blood sampling were performed on D5, D7, D10, D13, D17, and D21, with pregnancy diagnosis on D60. hCG treatment did not affect pregnancy rates. Accessory corpora lutea (aCL) appeared in 66.6 % (hCG-5) and 90.9 % (hCG-7) (P > 0.05). Total luteal area in hCG-7 was greater (P < 0.05) than in controls from D10 to D21. The mean original corpora lutea (oCL) area in hCG-7 was largest (P < 0.05) on D10. High-velocity Doppler area (HVDA) of aCL was greater (P < 0.05) in hCG-5 compared with hCG-7 on D13. Plasma progesterone (P4) concentrations were higher (P < 0.05) in hCG-7 than in controls from D10 to D21. Although hCG administered on D5 or D7 transiently enhanced luteal function and vascularization, it did not improve pregnancy outcomes in seasonally anovular dairy goats.

查看原文本刊更多论文

诱导发情开始后5或7天给予hCG对季节性无卵奶山羊黄体形态和功能的影响

目的是评价发情后第5天（D5）或第7天（D7）肌注人绒毛膜促性腺激素（hCG）对非繁殖期发情诱导奶山羊黄体结构（数量、面积、血管化和黄体酮分泌）和妊娠率的影响。34只高山山羊接受含有醋酸甲孕酮的阴道内海绵治疗6天，并在海绵取出前24 小时单次静脉注射200国际单位（IU）马绒毛膜促性腺激素（eCG）加37.5微克（µg） d-氯前列醇。所有都表现出发情，并由肥沃的雄鹿交配。山羊被随机分配给hCG-5 (D5上300 IU)；n = 12),hCG-7 (300 IU on D7；N = 11)或对照组（N = 11）。于D5、D7、D10、D13、D17、D21行经直肠卵巢超声及采血，D60诊断妊娠。hCG治疗不影响妊娠率。副黄体（aCL）发生率分别为66.6% % （hCG-5）和90.9% % (hCG-7) （P >； 0.05）。从D10到D21， hCG-7组黄体总面积大于对照组（P <； 0.05）。hCG-7的平均原始黄体（oCL）面积在D10时最大（P <； 0.05）。D13时hCG-5的aCL高速多普勒面积（HVDA）大于hCG-7 （P <； 0.05）。从D10到D21， hCG-7组血浆孕酮（P4）浓度高于对照组（P <； 0.05）。虽然在D5或D7上使用hCG可以短暂地增强黄体功能和血管化，但它并没有改善季节性无卵奶山羊的妊娠结局。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Animal Reproduction Science 农林科学-奶制品与动物科学

CiteScore

4.50

自引率

9.10%

发文量

136

审稿时长

54 days

期刊介绍： Animal Reproduction Science publishes results from studies relating to reproduction and fertility in animals. This includes both fundamental research and applied studies, including management practices that increase our understanding of the biology and manipulation of reproduction. Manuscripts should go into depth in the mechanisms involved in the research reported, rather than a give a mere description of findings. The focus is on animals that are useful to humans including food- and fibre-producing; companion/recreational; captive; and endangered species including zoo animals, but excluding laboratory animals unless the results of the study provide new information that impacts the basic understanding of the biology or manipulation of reproduction. The journal''s scope includes the study of reproductive physiology and endocrinology, reproductive cycles, natural and artificial control of reproduction, preservation and use of gametes and embryos, pregnancy and parturition, infertility and sterility, diagnostic and therapeutic techniques. The Editorial Board of Animal Reproduction Science has decided not to publish papers in which there is an exclusive examination of the in vitro development of oocytes and embryos; however, there will be consideration of papers that include in vitro studies where the source of the oocytes and/or development of the embryos beyond the blastocyst stage is part of the experimental design.