Animal Reproduction Science最新文献

{"title":"Unmixing the [Fluorescent] Spectrum of Sperm Cellular Health Utilizing High-Parameter Image-based, Spectral Flow Cytometry","authors":"Mubashrah Mahmood, Karl Kerns","doi":"10.1016/j.anireprosci.2025.107912","DOIUrl":"10.1016/j.anireprosci.2025.107912","url":null,"abstract":"","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"278 ","pages":"Article 107912"},"PeriodicalIF":2.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144570380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sarcoplasmic/Endoplasmic Reticulum Ca2+-ATPase (SERCA) is involved in the maintenance of pig semen at 17°C","authors":"Ferran Garriga , Jesús Martínez-Hernández , Ainhoa Parra-Balaguer , Marc Llavanera , Joan E. Rodríguez-Gil , Marc Yeste","doi":"10.1016/j.anireprosci.2025.107903","DOIUrl":"10.1016/j.anireprosci.2025.107903","url":null,"abstract":"","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"278 ","pages":"Article 107903"},"PeriodicalIF":2.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144571991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Speed vs Endurance: The importance of assessing sperm motility after storage","authors":"Pedro Sá , Rodrigo M. Godinho , Marta Gòdia , Claudia A. Sevillano , Barbara Harlizius , Ole Madsen , Henk Bovenhuis","doi":"10.1016/j.anireprosci.2025.107893","DOIUrl":"10.1016/j.anireprosci.2025.107893","url":null,"abstract":"","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"278 ","pages":"Article 107893"},"PeriodicalIF":2.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144572093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Divergence in the circulating small non-coding RNA transcriptome based on female pig fertility status","authors":"Lauren Fletcher ,&nbsp;Xiaoshu Zhan ,&nbsp;Riddhi Chabrotra ,&nbsp;Julang Li","doi":"10.1016/j.anireprosci.2025.107948","DOIUrl":"10.1016/j.anireprosci.2025.107948","url":null,"abstract":"<div><div>The reproductive performance of sows is critical to pork industry productivity. However, genetic improvement of reproductive and litter traits remains challenging due to low heritability and unfavourable genetic correlations. Integrating small non-coding (sncRNA) biomarkers to supplement existing selection protocols may enhance female pig reproductive potential selection. This study aimed to compare circulating serum sncRNA profiles between infertile (INF; nonpregnant after two consecutive artificial inseminations, <em>n</em> = 30) and high reproductive potential (HRP; average lifetime live litter size ≥ 13 piglets, <em>n</em> = 50) female pigs and identify candidate miRNA biomarkers associated with reproductive potential. Small RNA sequencing revealed 19 significantly (<em>P</em> &lt; 0.05) different expressed sncRNAs (12 upregulated and 7 downregulated in INF) and 13 significantly (<em>P</em> &lt; 0.05) different expressed miRNAs (8 upregulated and 5 downregulated in INF). Many of the identified DEmiRNAs have known roles in female reproduction and infertility, however, whether these differences are causal or compensatory responses of female pig reproductive potential level remains unclear. Using a complementary feature selection approach, 22 candidate miRNA biomarkers were selected, including miR-140-3p, miR-215-5p, and miR-7142-3p. The trained support vector machine (SVM) classified female pig reproductive potential with high accuracy (91 %), sensitivity for HRP (96 %) and specificity for INF (83 %), supporting its potential to improve selection efficiency of reproductive potential in the breeding herd. Validation of these 22 candidate miRNA biomarkers and model in a larger, independent cohort of female pigs is necessary to determine generalizability and use for practical application in on-farm breeding herd selection.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"279 ","pages":"Article 107948"},"PeriodicalIF":2.2,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144595518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic characteristics of follicular fluid in cows: A review","authors":"Canqiang Lu ,&nbsp;Yun Feng ,&nbsp;Zhengda Li ,&nbsp;Deshun Shi ,&nbsp;Fenghua Lu","doi":"10.1016/j.anireprosci.2025.107872","DOIUrl":"10.1016/j.anireprosci.2025.107872","url":null,"abstract":"<div><div>Female fertility largely depends on ovarian function. Follicles serve as the basic unit of the ovary and provide the metabolic microenvironment for oocyte development. The metabolism of follicular fluid changes dynamically with follicular development and is affected by maternal metabolism, age, and breeding season. This paper aims to summarize the metabolic characteristics of follicular fluid in growing cohort follicles, ovulatory follicles, and cystic follicles and discuss changes in follicular fluid metabolism associated with maternal metabolic stress, maternal age, and breeding season. Results provide a systematic and clear understanding of the metabolic characteristics of bovine follicular development.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"279 ","pages":"Article 107872"},"PeriodicalIF":2.2,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144490825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effect of β-carotene against lipopolysaccharide-induced meiotic maturation defects in porcine cumulus-oocytes complexes","authors":"Miaoyu Chen,&nbsp;Jia Li,&nbsp;Yijing He,&nbsp;Weihan Wang,&nbsp;Shiqiang Ju,&nbsp;Qiao Li","doi":"10.1016/j.anireprosci.2025.107947","DOIUrl":"10.1016/j.anireprosci.2025.107947","url":null,"abstract":"<div><div>Gram-negative bacterial infections are a leading cause of ovarian dysfunction and fertility impairment, primarily due to the adverse effects of the endotoxin lipopolysaccharide (LPS). β-Carotene possesses potent anti-inflammatory and anti-apoptotic properties. This study aimed to evaluate whether β-carotene could protect porcine cumulus-oocyte complexes (COCs) from LPS-induced damage. The results showed that 10 μg/mL LPS exposure inhibited cumulus expansion, reduced the extrusion rate of the first polar body (PB1), and induced meiotic defects, including spindle damage. Additionally, LPS treatment upregulated the expression of GRP78 and unfolded protein response (UPR)-related genes (<em>PERK</em>, <em>IRE1</em>, and <em>ATF4</em>) in cumulus cells. LPS exposure increased the proportion of apoptotic cells, upregulated the expression of the endoplasmic reticulum stress (ERS)-related apoptotic gene CHOP, and activated the downstream apoptotic program. In contrast, cotreatment with 5 μM β-carotene alleviated these adverse effects. Notably, β-carotene administration partially repaired the gap junction intercellular communication (GJIC) between cumulus cells and oocytes, as well as the density and quantity of transzonal projections (TZPs), and enhanced the expression of key proteins, including <em>FSCN1</em>, <em>DAAM1</em> and <em>MYO10</em>. In summary, β-carotene protects porcine COCs from LPS-induced reproductive toxicity by improving meiotic maturation, alleviating LPS-induced spindle defects, mitigating ER stress and apoptosis, and repairing TZPs' structure.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"279 ","pages":"Article 107947"},"PeriodicalIF":2.2,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144490362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Follicular fluid secretome enhances the meiotic competence of in vitro matured equine oocytes, but not the developmental competence of zygotes after intracytoplasmic sperm injection (ICSI)","authors":"M. Luis-Calero ,&nbsp;F. Marinaro ,&nbsp;CC Muñoz-García ,&nbsp;P. Fernández-Hernández ,&nbsp;JM Ortiz-Rodríguez ,&nbsp;R. Fernández-González ,&nbsp;E. Pericuesta ,&nbsp;A. Gutiérrez-Adán ,&nbsp;L. González-Fernández ,&nbsp;B. Macías-García","doi":"10.1016/j.anireprosci.2025.107946","DOIUrl":"10.1016/j.anireprosci.2025.107946","url":null,"abstract":"<div><div><em>In vitro</em> embryo production is widely used in equine clinical reproduction; however, its efficiency remains suboptimal due to the undermined developmental competence of <em>in vitro-</em>matured oocytes. In this work, we evaluated the effect of mare preovulatory follicular fluid (PFF) secretome supplementation during <em>in vitro</em> maturation (IVM) of equine oocytes recovered <em>post-mortem</em>. Secretome was isolated from PFF and was added to the IVM medium at concentrations of 20 µg/ml (S20) or 40 µg/ml (S40). The percentage of mature oocytes, as well as embryo cleavage, blastocyst development, and gene expression of embryos produced by intracytoplasmic sperm injection (ICSI) was analysed. Gene expression of embryos at day 7 post-ICSI was analysed by real-time quantitative PCR. We observed that the addition of PFF secretome at 40 µg/ml (S40) significantly increased oocyte maturation rates (Control vs. S40: 35.4 ± 3.6 % vs. 46.5 ± 2.7 %; mean % ± SEM; <em>P</em> &lt; 0.05) and reduced the proportion of degenerated oocytes after maturation (Control vs. S40: 58.6 ± 4.2 % vs. 44.0 ± 4.8 %; <em>P</em> &lt; 0.05) compared to the control group. However, no effects were observed on cleavage or blastocyst rates, nor on the expression of the candidate genes studied, namely <em>BEX2, FABP3, ODC, MOBKL3, HSP90AA1, BAX</em> and <em>BCL2.</em> In conclusion, supplementation of PFF secretome during IVM at 40 µg/ml, but not at 20 µg/ml, improves the meiotic competence of <em>post-mortem</em> retrieved equine oocytes but does not influence their developmental competence or the expression of the selected genes in the resulting embryos.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"279 ","pages":"Article 107946"},"PeriodicalIF":2.2,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144365062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The combination of FLI, ITS, pFF, and citrate improves the developmental potential of gilt oocytes during in vitro maturation","authors":"Zhilong Chen ,&nbsp;Caiyong Zhang ,&nbsp;Chao Yan ,&nbsp;Cuiting Peng ,&nbsp;Hao Xie ,&nbsp;Yingting Pan ,&nbsp;Lin Qi ,&nbsp;Chaoqun Qu ,&nbsp;Yulan Zhao ,&nbsp;Zhonglin Tang","doi":"10.1016/j.anireprosci.2025.107945","DOIUrl":"10.1016/j.anireprosci.2025.107945","url":null,"abstract":"<div><div>The intrinsic quality of porcine oocytes is a critical determinant of early embryonic development during <em>in vitro</em> production (IVP). This study investigated the effects of chemically defined <em>in vitro</em> maturation (IVM) systems supplemented with glutamine, GlutaMAX, insulin-transferrin-selenium (ITS), porcine follicular fluid (pFF), citrate, and growth factors (FGF2, LIF, IGF1, termed FLI) on the cumulus diffusion and maturation of oocytes derived from different diameters follicles. Moreover, four IVM media were systematically assessed for their effects on oocyte nuclear and cytoplasmic maturation and subsequent embryonic development. Results showed that the addition of pFF or FLI alone significantly enhanced cumulus expansion (<em>P</em> &lt; 0.05). FLI medium combined with ITS, citrate, and pFF, exhibited higher average cumulus diffusion diameters and rates of first polar body extrusion. Notably, the synergistic combination of ITS, citrate, and pFF with FLI medium upregulated expression of cumulus expansion makers (<em>HAS2</em>, <em>PTX3</em>, <em>CX43</em>, <em>TNFAIP6</em>) and oocyte maturation makers (<em>GDF9</em> and <em>BMP15</em>), improved cortical granule distribution, spindle morphology, chromosome alignment, and elevated glutathione (GSH) levels. This combination also enhanced cleavage and blastocyst rates, increased inner cell mass cell counts in blastocysts, and improved enucleation and fusion efficiency of oocytes following somatic cell nuclear transfer (SCNT). Collectively, the combination of FLI medium with ITS, pFF, and citrate synergistically promoted the maturation and developmental potential of porcine oocytes derived from prepubertal gilts, including those from small follicles. This optimized IVM system provides a robust foundation for advancing porcine IVP in reproductive biotechnology.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"279 ","pages":"Article 107945"},"PeriodicalIF":2.2,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144320827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Age-related changes in semen characteristics of selected Slovak geese breeds","authors":"Eva Tvrdá ,&nbsp;Cyril Hrnčár ,&nbsp;Filip Benko ,&nbsp;Michal Ďuračka ,&nbsp;Anton Kováčik ,&nbsp;Jakub Vozaf ,&nbsp;Andrea Svoradová ,&nbsp;Henrieta Arpášová ,&nbsp;Miroslava Kačániová ,&nbsp;Peter Chrenek","doi":"10.1016/j.anireprosci.2025.107888","DOIUrl":"10.1016/j.anireprosci.2025.107888","url":null,"abstract":"<div><div>This paper strove to characterize and compare ejaculates collected from two geese breeds over the period of two years in the context of selected traditional, biochemical, oxidative, bacteriological, and immunological properties. Slovak white and Suchovy ganders were selected for sperm quality evaluation, while bacteria were characterized using the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Oxidative profile of semen was assessed using chemiluminescent and colorimetric protocols. Levels of pro-inflammatory cytokines and antibacterial proteins were evaluated with the enzyme-linked immunosorbent assay. Seminal plasma biochemistry was assessed with an automated clinical chemistry analyzer. Overall, lower semen quality was found in the Slovak white breed and during the first year of semen collection in both breeds. At the same time, the sperm motility was in a strong positive correlation with the plasma membrane integrity (<em>P</em> &lt; 0.0001), whilst being negatively associated with the bacterial load (<em>P</em> = 0.0056), reactive oxygen species production (<em>P</em> &lt; 0.0001), DNA fragmentation (<em>P</em> &lt; 0.0001) and the levels of interleukin-1 (<em>P</em> &lt; 0.0001) and C-reactive protein (<em>P</em> &lt; 0.0001). Higher semen quality in ganders was accompanied by increased seminal plasma levels of lysozyme (<em>P</em> &lt; 0.0001), cathelicidin (<em>P</em> &lt; 0.0001), calcium (<em>P</em> &lt; 0.01), magnesium (<em>P</em> &lt; 0.01 in the case of the Suchovy breed), phosphorus (<em>P</em> &lt; 0.001), total proteins (<em>P</em> &lt; 0.0001 with respect to the Slovak white breed; <em>P</em> &lt; 0.01 with regards to the Suchovy breed) and cholesterol (<em>P</em> &lt; 0.05), and a decreased activity of aspartate transaminase (<em>P</em> &lt; 0.0001). We may conclude that semen of overall better quality was obtained during the second year of semen collection as opposed to the first year in ganders of both selected goose breeds.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"279 ","pages":"Article 107888"},"PeriodicalIF":2.2,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144279624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An analysis addressing factors affecting risk of ovulation, pregnancy, and pregnancy loss in lactating dairy cows submitted to fixed-time artificial insemination programs with special reference to the diameter of the ovarian follicle","authors":"Jeffrey S. Stevenson","doi":"10.1016/j.anireprosci.2025.107887","DOIUrl":"10.1016/j.anireprosci.2025.107887","url":null,"abstract":"<div><div>Because reduced fertility in studies resulted from GnRH-induced ovulation of small or large follicles in lactating dairy cattle, the objective was to determine the association of follicle diameter and risks of ovulation and pregnancy per artificial insemination (P/AI). An analysis was conducted of data from 14 studies in 82 herd-year-seasons in which ovulation was synchronized before a fixed-time AI. Follicle diameter was included as a continuous variable in a random-effects mixed model that used herd-year-season as a random surrogate for study. Effects of presynchronization, parity, cycling status, body condition score, occurrence of estrus, service sire, and progesterone were included as appropriate in initial models plus all two-way interactions. Ovulation risk after first GnRH treatment of Ovsynch increased (<em>P</em> &lt; 0.001) with increasing follicle diameter. Ovulation risk after the breeding GnRH treatment increased (<em>P</em> = 0.03) in multiparous but not in primiparous cows as follicle diameter increased. Pregnancy per AI at d 28–37 and at d 56–84 was not associated with follicle diameter but was affected by presynchronization treatment (<em>P</em> = 0.03), ovulation after the breeding GnRH treatment (<em>P</em> = 0.003), parity (<em>P</em> &lt; 0.001), prebreeding cycling status (<em>P</em> &lt; 0.001), and progesterone (<em>P</em> &lt; 0.001) before AI. As follicle diameter before AI increased, pregnancy loss by d 56–84 after AI decreased (<em>P</em> = 0.006), especially in cows of greater body condition. These results do not support any effect of follicle size on P/AI in cows whose ovulation is synchronized before AI, but less pregnancy loss was associated with increasing follicle diameter.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"279 ","pages":"Article 107887"},"PeriodicalIF":2.2,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144307760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}