Animal Reproduction Science最新文献_第10页

Supplementation of l-arginine in pregnant gilts affects the protein abundance of DNMT1 in 35-day fetuses 妊娠母猪补充 L-精氨酸会影响 35 天胎儿中 DNMT1 蛋白的丰度

IF 2.2 2区农林科学

Animal Reproduction Science Pub Date : 2024-08-08 DOI: 10.1016/j.anireprosci.2024.107574

Karine Assis Costa , Lívia Maria dos Reis Barbosa , Daniele Botelho Diniz Marques , Walmir da Silva , Breno Soares Camilo , Domingos Lollobrigida de Souza Netto , Alysson Saraiva , José Domingos Guimarães , Simone Eliza Facioni Guimarães

{"title":"Supplementation of l-arginine in pregnant gilts affects the protein abundance of DNMT1 in 35-day fetuses","authors":"Karine Assis Costa , Lívia Maria dos Reis Barbosa , Daniele Botelho Diniz Marques , Walmir da Silva , Breno Soares Camilo , Domingos Lollobrigida de Souza Netto , Alysson Saraiva , José Domingos Guimarães , Simone Eliza Facioni Guimarães","doi":"10.1016/j.anireprosci.2024.107574","DOIUrl":"10.1016/j.anireprosci.2024.107574","url":null,"abstract":"<div>Maternal nutrition is one of the main environmental factors regulating gene expression during fetal development through epigenetic modifications. Some nutrients, such as the amino acid l-arginine, are added to maternal diets to modulate gene expression, improve the reproductive performance of females, and enhance conceptus development. This study investigated the hypothesis that supplementation of pregnant gilts with l-arginine regulates gene expression in conceptuses through epigenetic mechanisms. For this, fetal programming phenotypic markers, the expression of key epigenetic genes, and the abundance of DNA methylation proteins (DNMT3A and DNMT1) were evaluated in 25- and 35-day conceptuses from gilts supplemented (ARG) or not (CON) with 1.0 % l-arginine during early gestation. At 25 days, there were no significant differences in phenotypic markers between CON and ARG embryos (P > 0.05). Similarly, no differences were found between CON and ARG fetuses at 35 days (P > 0.05). Maternal supplementation with l-arginine did not influence the expression of the evaluated key epigenetic genes in pig embryos or fetuses, nor DNMT3A protein abundance (P > 0.05); on the other hand, DNMT1 protein abundance was lower in ARG fetuses (P = 0.002). It is concluded that supplementation of l-arginine in pregnant gilts affects epigenetic mechanisms, such as DNA methylation, in 35-day fetuses through regulation of DNMT1 levels. Further studies using transcriptomic and proteomic analysis could reveal additional epigenetic modifications in embryos and fetuses following maternal supplementation with l-arginine.</div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"270 ","pages":"Article 107574"},"PeriodicalIF":2.2,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142011844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Significance of fatty acids in fish broodstock nutrition 脂肪酸在鱼苗营养中的重要性。

IF 2.2 2区农林科学

Animal Reproduction Science Pub Date : 2024-08-07 DOI: 10.1016/j.anireprosci.2024.107573

Thangaraju Thiruvasagam , Pushparaj Chidambaram , Amit Ranjan , N.B. Komuhi

{"title":"Significance of fatty acids in fish broodstock nutrition","authors":"Thangaraju Thiruvasagam , Pushparaj Chidambaram , Amit Ranjan , N.B. Komuhi","doi":"10.1016/j.anireprosci.2024.107573","DOIUrl":"10.1016/j.anireprosci.2024.107573","url":null,"abstract":"<div>The nutritional status of broodstock profoundly affects their reproductive performance and offspring survival. Studies on lipids and essential fatty acids in broodstock diets highlight their importance in cell structure, fecundity, fertilization, egg and larval quality, and providing metabolic energy for reproduction. Long-chain polyunsaturated fatty acids (Lc-PUFA) like DHA (22:6 n-3) and EPA (20:5 n-3) are vital for egg and larval development, while arachidonic acid (ARA) produces eicosanoids essential for reproduction. The fatty acid requirements vary by habitat; freshwater fish typically lack ∆12 and ∆15 desaturase enzymes to convert oleic acid into vital polyunsaturated fatty acids like linoleic and linolenic acids but can synthesize linoleic (18:2 n-6) and linolenic (18:3 n-3) into Lc-PUFAs such as EPA, DHA, and ARA through desaturation and elongation, whereas marine teleost cannot. Hence, broodstock feed fatty acid composition must be tailored by incorporating ingredients with a specific fatty acid composition to enhance reproductive performance. This review provides updated information on fatty acid supplementation in broodstock diets to improve reproductive outcomes in commercially important finfish, offering valuable insights for researchers, academicians, hatchery owners, and fish farmers to produce better-quality seeds.</div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"268 ","pages":"Article 107573"},"PeriodicalIF":2.2,"publicationDate":"2024-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141915952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thawing of cryopreserved sperm from domestic animals: Impact of temperature, time, and addition of molecules to thawing/insemination medium 家畜冷冻保存精子的解冻：温度、时间和在解冻/授精介质中添加分子的影响。

IF 2.2 2区农林科学

Animal Reproduction Science Pub Date : 2024-08-03 DOI: 10.1016/j.anireprosci.2024.107572

Felipe Pezo , María José Contreras , Fabiola Zambrano , Pamela Uribe , Jennie Risopatron , Andre Furugen Cesar de Andrade , Marc Yeste , Raúl Sánchez

{"title":"Thawing of cryopreserved sperm from domestic animals: Impact of temperature, time, and addition of molecules to thawing/insemination medium","authors":"Felipe Pezo , María José Contreras , Fabiola Zambrano , Pamela Uribe , Jennie Risopatron , Andre Furugen Cesar de Andrade , Marc Yeste , Raúl Sánchez","doi":"10.1016/j.anireprosci.2024.107572","DOIUrl":"10.1016/j.anireprosci.2024.107572","url":null,"abstract":"<div>In recent decades, there has been a growing interest in optimizing the protocols intended to sperm cryopreservation in domestic animals. These protocols include initial cooling, freezing, and thawing. While different attempts have been devised to improve sperm cryopreservation, the efficiency of this reproductive biotechnology is still far from being optimal. Furthermore, while much attention in improving cooling/freezing, less emphasis has been made in how thawing can be ameliorated. Despite this, the conditions through which, upon thawing, sperm return to physiological temperatures are much relevant, given that these cells must travel throughout the female genital tract until they reach the utero-tubal junction. Moreover, the composition of the media used for artificial insemination (AI) may also affect sperm survival, which is again something that one should bear because of the long journey that sperm must make. Furthermore, sperm quality and functionality decrease dramatically during post-thawing incubation time. Added to that, the deposition of the thawed sperm suspension devoid of seminal plasma in some species during an AI is accompanied by a leukocyte migration to the uterine lumen and with it the activation of immune mechanisms. Because few reviews have focused on the evidence gathered after sperm thawing, the present one aims to compile and discuss the available information concerning ruminants, pigs and horses.</div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"268 ","pages":"Article 107572"},"PeriodicalIF":2.2,"publicationDate":"2024-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141915953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metagenomic identification of bull semen microbiota in different seasons 不同季节公牛精液微生物群的元基因组鉴定。

IF 2.2 2区农林科学

Animal Reproduction Science Pub Date : 2024-07-30 DOI: 10.1016/j.anireprosci.2024.107569

Aleksandar Cojkic , Adnan Niazi , Jane M. Morrell

{"title":"Metagenomic identification of bull semen microbiota in different seasons","authors":"Aleksandar Cojkic , Adnan Niazi , Jane M. Morrell","doi":"10.1016/j.anireprosci.2024.107569","DOIUrl":"10.1016/j.anireprosci.2024.107569","url":null,"abstract":"<div>A seasonal effect on sperm quality parameters was observed previously. Although identification of the bull semen microbiota by 16S rRNA sequencing was performed previously, it has not been carried out in commercial semen samples from different seasons, and its connection with sperm quality parameters has not been evaluated yet. The objectives in this study were; (i) to evaluate diversity of bull semen microbiota and sperm quality parameters in different seasons, and (ii) to find if specific bacteria were associated with seasonal differences in specific sperm quality parameters. Bull semen microbiota was identified in 54 commercial bull semen samples from 3 seasons (winter, spring, summer). Sperm quality was analysed by Computer Assisted Sperm Analyses (CASA) and Flow Cytometry (FC). From 28 phyla in all samples, six phyla were identified in samples from all seasons, with observed seasonal differences in their distribution. At genus level, 388 genera were identified, of which 22 genera had a relative abundance over 1 % and showed seasonal differences in bacterial diversity, and 9 bacteria genera were present in all seasons. Differences between spring and summer (P < 0.05) were observed for live hydrogen peroxide positive sperm cells. A trend towards significance (0.10 > P > 0.05) was observed for some CASA kinematics (VCL and LIN) and FC parameters (High respiratory activity, and live hydrogen peroxide positive sperm cells) between seasons. Nevertheless, associations between sperm quality parameters and specific bacteria were observed in spring.</div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"268 ","pages":"Article 107569"},"PeriodicalIF":2.2,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S037843202400160X/pdfft?md5=6a6ad7e41ee382c9ba2ed473f985b699&pid=1-s2.0-S037843202400160X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141888212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Infectious and non-infectious causes for pregnancy loss in South American camelids – A review 南美洲驼科动物妊娠失败的感染性和非感染性原因 - 综述。

IF 2.2 2区农林科学

Animal Reproduction Science Pub Date : 2024-07-29 DOI: 10.1016/j.anireprosci.2024.107571

Hannah Eggimann , Isabelle Rediger , Gaby Hirsbrunner , Patrik Zanolari

引用次数: 0

Livestock-Forest integrated system attenuates deleterious heat stress effects in bovine oocytes 家畜-森林综合系统可减轻牛卵母细胞中有害的热应力效应

IF 2.2 2区农林科学

Animal Reproduction Science Pub Date : 2024-07-26 DOI: 10.1016/j.anireprosci.2024.107568

Clara Slade Oliveira , Hugo Rocha Sabença Dias , Agostinho Jorge dos Reis Camargo , Anderson Mourão , Viviane Luzia da Silva Feuchard , Marcelo Dias Muller , Felipe Zandonadi Brandão , Luiz Altamiro Garcia Nogueira , Rui da Silva Verneque , Naiara Zoccal Saraiva , Luiz Sergio de Almeida Camargo

{"title":"Livestock-Forest integrated system attenuates deleterious heat stress effects in bovine oocytes","authors":"Clara Slade Oliveira , Hugo Rocha Sabença Dias , Agostinho Jorge dos Reis Camargo , Anderson Mourão , Viviane Luzia da Silva Feuchard , Marcelo Dias Muller , Felipe Zandonadi Brandão , Luiz Altamiro Garcia Nogueira , Rui da Silva Verneque , Naiara Zoccal Saraiva , Luiz Sergio de Almeida Camargo","doi":"10.1016/j.anireprosci.2024.107568","DOIUrl":"10.1016/j.anireprosci.2024.107568","url":null,"abstract":"<div>Global warming poses significant challenges to the fertility of tropical dairy cattle. One promising approach to mitigate heat stress effects on reproductive function and reduce the carbon footprint is the use of integrated livestock-forest (ILF) systems. The aim of this study was to investigate the effects of two different systems, namely Full Sun (FS) and ILF, on maternal hyperthermia and oocyte quality of Holstein and Girolando heifers during the tropical summer season. The temperature-humidity index (THI) data revealed intense heat stress during the experiment. Both the system (P<0.01) and the breed (P<0.01) factors had a significant impact on vaginal temperature, being hyperthermia more pronounced in the FS system and in the Holstein breed. Over the five time points collected at a 33-day interval, we observed distinct patterns for ILF (P=0.65) and FS (P<0.001) systems, suggesting an adaptive response in animals kept in FS systems. Furthermore, oocyte quality assessment revealed an effect of the system for oocyte diameter (P<0.001) and levels of IGFBP2 (P<0.001), and caspase 3 levels showed a decrease in ILF compared to FS for both Holstein (P<0.001) and Girolando (P<0.001) breeds. Collectively, these parameters indicate that oocyte quality during the summer months was superior in animals maintained in the ILF system. In conclusion, the ILF system demonstrated promising results in attenuating maternal hyperthermia and mitigating its effects on oocyte quality. Additionally, our observations suggest that animals in the FS system may exhibit an adaptive response to heat stress.</div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"268 ","pages":"Article 107568"},"PeriodicalIF":2.2,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141841765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent strategies to mitigate reproductive aging in male broiler breeders: A review 缓解雄性肉种鸡生殖衰老的最新策略：综述。

IF 2.2 2区农林科学

Animal Reproduction Science Pub Date : 2024-07-26 DOI: 10.1016/j.anireprosci.2024.107570

Mahdi Ansari

{"title":"Recent strategies to mitigate reproductive aging in male broiler breeders: A review","authors":"Mahdi Ansari","doi":"10.1016/j.anireprosci.2024.107570","DOIUrl":"10.1016/j.anireprosci.2024.107570","url":null,"abstract":"<div>The continued improvement of genetics, nutrition, and management has resulted in rapid growth, better feed efficiency, and higher meat yield with competitive prices in the broiler industry. Nowadays, however, it is well-documented that productive traits and fertility are negatively correlated, and male broiler breeders are exposed to a fertility decline after 45 wk of age. Considering a low male-to-female ratio in breeder flocks, roosters have a prominent impact on flock fertility. Consequently, strategies to maintain the fertility of male broiler breeders could guarantee the reproductive performance of commercial herds. Understanding reproductive aging demands deep insights into its molecular and physiological mechanisms. Over-weighting, Sertoli and Leydig cell dysfunctions, compromised antioxidant capacity, imbalance in sexual hormones, and epididymal lithiasis are among candidate culprits associated with reproductive aging in roosters. Nutritional and managing strategies have been successfully applied to modulate body weight, improve sperm fatty acid profile and antioxidant status, and boost spermatogenic and steroidogenic pathways. The current review characterizes the physiology and biochemistry of reproductive aging in male broiler breeders and then highlights strategies and their underlying mechanisms to mitigate this failure. In summary, applying one or more of the abovementioned strategies might result in consistent post-peak reproduction and benefit producers in the poultry industry.</div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"268 ","pages":"Article 107570"},"PeriodicalIF":2.2,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141787086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrasonography for the detection of pregnancy and study of embryonic and fetal development in camels, buffaloes, and sheep: Techniques, equations, and limitations 用于检测骆驼、水牛和绵羊妊娠以及研究胚胎和胎儿发育的超声波检查：技术、公式和局限性

IF 2.2 2区农林科学

Animal Reproduction Science Pub Date : 2024-07-21 DOI: 10.1016/j.anireprosci.2024.107566

Ahmed Ali , Derar R. Derar , Abdel-Razek Kh. Abdel-Razek

{"title":"Ultrasonography for the detection of pregnancy and study of embryonic and fetal development in camels, buffaloes, and sheep: Techniques, equations, and limitations","authors":"Ahmed Ali , Derar R. Derar , Abdel-Razek Kh. Abdel-Razek","doi":"10.1016/j.anireprosci.2024.107566","DOIUrl":"10.1016/j.anireprosci.2024.107566","url":null,"abstract":"<div>New technologies for detecting pregnancy shortly after mating/insemination and identifying gestational age are essential for speeding up the reproductive cycle and ensuring high reproductive efficiency in livestock farming. Ultrasonography can successfully identify pregnancy and determine gestational age in many domestic animals. On the other hand, many herds of camel and buffalo and flocks of sheep are aware of the day of service, making it difficult to appropriately manage pregnant animals. This study provides a review of the literature on various techniques for ultrasonographically diagnosing pregnancy in camels, buffaloes, and sheep, focusing on the most appropriate times to use each technique, the earliest opportunity to diagnose pregnancy, and the possibility of using various parts of the fetus to create mathematical equations to determine gestational age. Some limitations of ultrasonography in pregnancy diagnosis were identified and significant pregnancy events in dromedaries were discussed, including left-horn and twin pregnancies. The data presented here will prove essential for researchers, farmers, and countries that rely heavily on these animals for providing meat, milk, cosmetics, and other animal products to enhance reproduction and production efficiency.</div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"268 ","pages":"Article 107566"},"PeriodicalIF":2.2,"publicationDate":"2024-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141841445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oxylipin profiling analyses reveal that ω-3 PUFA is more susceptible to lipid oxidation in sheep testis under oxidative stress 氧化脂分析表明，ω-3 PUFA 在绵羊睾丸氧化压力下更容易被脂质氧化。

IF 2.2 2区农林科学

Animal Reproduction Science Pub Date : 2024-07-20 DOI: 10.1016/j.anireprosci.2024.107567

Wanhong Li , Ting Yao , Xinyue Zhang , Xiuxiu Weng , Fadi Li , Xiangpeng Yue

{"title":"Oxylipin profiling analyses reveal that ω-3 PUFA is more susceptible to lipid oxidation in sheep testis under oxidative stress","authors":"Wanhong Li , Ting Yao , Xinyue Zhang , Xiuxiu Weng , Fadi Li , Xiangpeng Yue","doi":"10.1016/j.anireprosci.2024.107567","DOIUrl":"10.1016/j.anireprosci.2024.107567","url":null,"abstract":"<div>Reactive oxygen species causes oxidative stress, which oxidizes polyunsaturated fatty acids (PUFAs) to form oxidative metabolites. Sertoli cell is an important cellular metabolism of PUFA in testicular cells, and it regulates the testis development and spermatogenesis. However, the oxylipins generated in testes with different developmental statuses are lacking. In this study, twelve 6-month-old Hu sheep were selected and divided into large testicular group (L) and the small testicular group (S) (n=6). UPLC–MS/MS was conducted to screen oxylipins in the testis, and the total oxylipin and ω-3 PUFA-derived oxylipin contents in the S group were higher. A total of 20 differential oxylipins between the two groups were screened. Among them, the contents of ω-3 PUFA, DHA-derived oxylipins were increased in the S group. The arachidonic acid-derived oxylipin was lower in the S group. The mRNA expression levels of genes related to oxylipin regulation (AKR1B1, PTGER2, and PTGDS) were higher in the S group (P < 0.05). In vitro, 200 µM α-linolenic acid alleviated oxidative stress damage to Sertoli cells and improved cell viability by increasing the superoxide dismutase contents and mRNA expression levels of GPX4 and Bcl2. These results indicate that ω-3 PUFA is more susceptible to lipid oxidation in the S group under oxidative stress, which might alleviate the damage of oxidative stress to testis. Moreover, ALA could stimulate the proliferation of Sertoli cells by increasing the capacity of antioxidants. This work may provide a theoretical basis for further studies on the antioxidant properties of the testis for Hu sheep.</div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"268 ","pages":"Article 107567"},"PeriodicalIF":2.2,"publicationDate":"2024-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141787085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Brushing rams before and during electroejaculation improves sperm motility and kinetics with slight changes in stress biomarkers 在电射精前和电射精过程中刷洗公羊，可提高精子活力和动力学，并使应激生物标志物发生轻微变化

IF 2.2 2区农林科学

Animal Reproduction Science Pub Date : 2024-07-19 DOI: 10.1016/j.anireprosci.2024.107565

Juan Carlos Orihuela , Aline Freitas-de-Melo , Livia Pinto-Santini , Julia Giriboni , Florencia Beracochea , María Noel Viera , Rodolfo Ungerfeld

{"title":"Brushing rams before and during electroejaculation improves sperm motility and kinetics with slight changes in stress biomarkers","authors":"Juan Carlos Orihuela , Aline Freitas-de-Melo , Livia Pinto-Santini , Julia Giriboni , Florencia Beracochea , María Noel Viera , Rodolfo Ungerfeld","doi":"10.1016/j.anireprosci.2024.107565","DOIUrl":"10.1016/j.anireprosci.2024.107565","url":null,"abstract":"<div>The aim of this study was to determine whether brushing rams before and during electroejaculation (EE) reduces their stress response and improves the characteristics of the ejaculate. A single person brushed each ram for 5 min daily, for 15 days, in an individual pen. Semen was collected from five rams brushed before and during EE by the same brusher, while the other five were electroejaculated without being brushed. The treatments were exchanged three days later, so semen was collected from all the rams with both treatments. Brushing increased mass motility (P = 0.05), and curvilinear (P = 0.001), linear (P = 0.02), and average path (P = 0.01) velocities of sperm, as well as the average amplitude of lateral displacement of the sperm head (P = 0.05), and tended to increase sperm concentration (P = 0.09). Brushing tended to reduce the cortisol concentration (P = 0.06) and the duration of head movements when 2 V pulse series V was applied (P = 0.1). Brushing increased creatine kinase concentration (P = 0.04) and tended to increase rectal (P = 0.06) and maximum eye surface temperatures (P = 0.1), total time, and number of electrical pulses administered (P = 0.07 for both variables), as well as the sum of pulses per voltage applied during EE (P = 0.06). In rams accustomed to being brushed by the same person, brushing them before and during EE improved semen quality, with slight changes in the stress responses.</div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"268 ","pages":"Article 107565"},"PeriodicalIF":2.2,"publicationDate":"2024-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141850765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0