Animal Reproduction Science最新文献

{"title":"Determinants of ram sexual behavior and its impact on sheep breeding","authors":"Rodolfo Ungerfeld ,&nbsp;Brenda M. Alexander","doi":"10.1016/j.anireprosci.2024.107599","DOIUrl":"10.1016/j.anireprosci.2024.107599","url":null,"abstract":"<div><p>In sheep, anatomical characteristics of the cervix and animal value limit application of reproductive technologies; therefore, most breeding is natural service. Rams are selected based on desired physical traits and semen capacity, but their contribution to flock genetics is constrained by expression of sexual behavior. In 1964, it was first reported that approximately one-third of rams expressed limited sexual interest toward ewes in estrus. Therefore, if rams were evaluated for expression of sexual behavior, it is estimated that as many rams would be culled for the lack of behavior as are currently culled for semen quality or physical deficiencies. Sexual behavior is complex, requiring sensory recognition and processing, integration of ram cohorts and social dominance, female mate choice, and a physical response. This review of the literature aims to provide insights into factors influencing expression of ram sexual behavior.</p></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"270 ","pages":"Article 107599"},"PeriodicalIF":2.2,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142242840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasonographic quantification of the endometrium echogenicity and heterogeneity in canine physiological and pathological conditions using computer-assisted analysis","authors":"Sol Arioni ,&nbsp;Marlene Huk ,&nbsp;Pablo R. Batista ,&nbsp;Camila Lapuente ,&nbsp;Cristina Gobello ,&nbsp;Paula G. Blanco","doi":"10.1016/j.anireprosci.2024.107597","DOIUrl":"10.1016/j.anireprosci.2024.107597","url":null,"abstract":"<div><p>The aims of this study were: To ultrasonographically describe and compare endometrial echogenicity and heterogeneity using digital analysis in normal and bitches suffering from pyometra, cystic endometrial hyperplasia (CEH) and endometritis; and to evaluate the effect of clinical, bacteriological and histopathological uterine parameters on endometrial echogenicity and heterogeneity. Forty-one post pubertal intact bitches were included. According to clinical, ultrasonographic, anatomopathological and histopathological uterine evaluation, the animals were classified as: Pyometra (PYO; n=6); CEH (n=8); Endometritis (END; n=13); Normal group (NG; n=14). Endometrial images were analyzed with ImageJ software to obtain echogenicity and heterogeneity, represented as the mean gray value (MGV) and standard deviation of gray (SDG), respectively. The effect of the group, clinical, bacteriological, ultrasonographic and histological variables on MGV and SDG were analyzed by a generalized linear model. PYO exhibited higher MGV (P&lt;0.01) and SDG (P&lt;0.01) than the other groups. No differences were found among CEH, END and NG for both parameters (P&gt;0.1). Body weight decreased MGV (P&lt;0.01), while increasing degrees of inflammatory reaction (P&lt;0.01), edema (P&lt;0.01), hemorrhages (P&lt;0.01) and vascular congestion (P&lt;0.01) were associated with higher MGV. Inflammatory reaction (P&lt;0.01) and ulceration (P&lt;0.01) increased SDG. Ultrasonographic images evaluated using computer assisted image analysis were useful to differentiate pyometra from other uterine conditions in dogs. However, this technique could not differentiate among CEH, END and NG. Uterine echogenicity and echotexture, which clearly represent the different histopathological patterns, contribute to the diagnosis of the definite diagnosis of some canine uterine diseases.</p></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"270 ","pages":"Article 107597"},"PeriodicalIF":2.2,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142172381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of a sperm-Hyaluronan binding assay for evaluation of sperm quality in dromedary camels","authors":"A. Kotila-Ioannou ,&nbsp;JM Morrell ,&nbsp;T. Ntallaris ,&nbsp;M. Gargallo ,&nbsp;J.A. Skidmore ,&nbsp;C. Malo","doi":"10.1016/j.anireprosci.2024.107596","DOIUrl":"10.1016/j.anireprosci.2024.107596","url":null,"abstract":"<div><p>The objective of this study was to assess the ability of camel spermatozoa to bind in the Hyaluronan Binding Assay (HBA), to determine if conventional sperm quality parameters, in vitro fertilization capacity, and precursor of A-Kinase Anchoring Protein 4 (proAKAP4) values correlate with HBA results. The potential to predict post-thaw fertilization performance from HBA for fresh dromedary camel sperm was also evaluated. Semen samples were collected and assessed both fresh and post thawing, at 0 h and 1.5 h. Conventional semen analysis, HBA, and a proAKAP4 biomarker-test were used to validate sperm quality. A heterologous sperm penetration assay using zona pellucida-free goat oocytes was used to assess in vitro sperm fertilizing capacity. The results showed that dromedary camel spermatozoa bound to hyaluronan with no correlation between results from fresh samples and after thawing. Furthermore, the proAKAP4 test results showed a negative correlation with HBA at 0 h after thawing (r = - 0.62; P = 0.03). In the conventional analysis, only progressive motility (r = 0.65; P = 0.02) and straightness correlated with HBA for fresh semen (r = 0.69; P = 0.01). In the sperm penetration assay, a moderate but non-significant correlation was identified between fresh sperm HBA and penetration (r = 0.52; P = 0.07). In conclusion, results suggested that HBA can be used to assess camel sperm properties, but further investigation is needed to understand its correlation with other sperm quality parameters. The HBA score from fresh dromedary camel sperm was unable to predict post-thaw fertilization performance.</p></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"270 ","pages":"Article 107596"},"PeriodicalIF":2.2,"publicationDate":"2024-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142242838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of flunixin meglumine on the premature regression of corpus luteum, recovery rate, and embryo production in superovulated Dorper ewes","authors":"Ana Lucia Rosa e Silva Maia ,&nbsp;Lucas Machado Figueira ,&nbsp;Marcela Sene Rocha ,&nbsp;Jasmine Bantim de Souza Pinheiro ,&nbsp;Thais de Almeida Oliveira ,&nbsp;Felipe Zandonadi Brandão ,&nbsp;Jeferson Ferreira Fonseca ,&nbsp;Maria Emilia Franco Oliveira ,&nbsp;Claudio Alvarenga de Oliveira ,&nbsp;Joanna Maria Gonçalves Souza-Fabjan","doi":"10.1016/j.anireprosci.2024.107595","DOIUrl":"10.1016/j.anireprosci.2024.107595","url":null,"abstract":"<div><p>This study evaluated the use of flunixin meglumine to prevent the occurrence of premature corpus luteum (CL) regression in superovulated ewes, improving embryo recovery and viability. Ewes (<em>n</em>=23) submitted to conventional superovulatory protocol and laparoscopic artificial insemination were treated with 2.2 mg/kg/day of flunixin meglumine (FLU, <em>n</em>=12) or 1.5 mL saline solution (CONT, <em>n</em>=11) on Days 2, 3, and 4 (Day 0 = 48 h after device removal). Serum progesterone (P₄) concentrations were measured (Day 1–6). Ultrasound (US, Days 3 and 6) and laparoscopic evaluation (Day 6) were performed to identify luteinized structures. In the US, laparoscopy, and P₄ assessments, the percentage of ewes with premature CL regression differed (<em>P</em>&lt;0.05) between CONT (54.5; 63.6; and 54.5 %) and FLU (0.0; 0.0; and 0.0 %), respectively. The US exams revealed the effect (<em>P</em>&lt;0.05) of treatment on the number of regressing CL between CONT (1.4 ± 0.6) and FLU (0.0 ± 0.0). Greater (<em>P</em>&lt;0.05) number of normal CLs (10.5 ± 1.8 vs. 4.4 ± 1.5), ova/embryos (9.1 ± 2.1 vs. 3.7 ± 1.3), viable embryos (5.1 ± 1.1 vs. 2.6 ± 1.2), and recovery rate (79.5 ± 9.6 vs. 41.3 ± 15.0 %) were observed in FLU compared to CONT, respectively. The embryo viability rate did not differ (<em>P</em>&gt;0.05) between FLU (60.7 ± 10.5 %) and CONT (45.5 ± 16.1 %). In conclusion, the flunixin meglumine protocol was able to prevent the occurrence of premature CL regression in superovulated ewes, increasing the recovery rate and embryo production.</p></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"270 ","pages":"Article 107595"},"PeriodicalIF":2.2,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142139057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of commercial inactivated or modified-live virus vaccination at time of AI on corpus luteum development and function in beef cattle","authors":"K.M. Epperson ,&nbsp;J.J.J. Rich ,&nbsp;S. Menegatti Zoca ,&nbsp;L.K. Quail ,&nbsp;T.N. Andrews ,&nbsp;A.C. Kline ,&nbsp;F.J. White ,&nbsp;R.F. Daly ,&nbsp;R.A. Cushman ,&nbsp;A.P. Snider ,&nbsp;G.A. Perry","doi":"10.1016/j.anireprosci.2024.107594","DOIUrl":"10.1016/j.anireprosci.2024.107594","url":null,"abstract":"<div><p>Our objective was to evaluate the effect of vaccination with an inactivated virus vaccine (IVV) or modified-live virus (MLV) vaccine on the corpus luteum (CL). On d0, synchronized beef cows were treated with MLV (n = 70; BoviShield Gold FP5VL5), IVV (n = 16; ViraShield 6VL5HB), or were unvaccinated controls (n = 5). Plasma was collected from treated animals on d0 and every other day through d22. Plasma was analyzed for concentrations of progesterone and 15 cytokines. Between d10 and d13, selected females (n = 13) were ovariectomized; controls were slaughtered on d15/16 to obtain CL for histological evaluation. There were reduced numbers of large luteal cells (LLC) in MLV compared to IVV and controls (<em>P</em> &lt; 0.0001), but IVV were similar to controls (<em>P</em> = 0.11). MLV had decreased LLC percentage compared to controls, and IVV were intermediate (<em>P</em> &lt; 0.0001, MLV: 1.57 ± 0.33 %, IVV: 2.99 ± 0.30 %, Control: 6.45 ± 0.33 %). Based on progesterone concentrations, 24 % MLV and 0 % IVV had an abnormal cycle following vaccination. Overall, MLV had reduced progesterone concentrations (<em>P</em> = 0.02; MLV: 3.61 ± 0.22; IVV: 4.81 ± 0.46 ng/mL). The new CL that formed following an abnormal cycle in MLV had the greatest percentage (35.56 ± 5.5 %) of apoptotic cells. Treatment by cycle status interaction, and time significantly affected IFN-γ, IP-10, MIP-1β, and MCP-1 (<em>P</em> &lt; 0.03), with several time points having elevated concentrations in abnormally cycling MLV animals. Collectively, this demonstrates MLV vaccination around estrus negatively influenced LLC, progesterone, and increased luteal apoptosis and pro-inflammatory cytokines.</p></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"270 ","pages":"Article 107594"},"PeriodicalIF":2.2,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0378432024001854/pdfft?md5=d59855e58c7084504fc5dc958b55abf4&pid=1-s2.0-S0378432024001854-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142136265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"L-carnitine supplementation in conventional slow and ultra-rapid freezing media improves motility, membrane integrity, and fertilizing ability of dog epididymal sperm","authors":"A.E. Ramón-López ,&nbsp;J.P. Fernández-Collahuazo ,&nbsp;J.X. Samaniego ,&nbsp;J.M. Duma ,&nbsp;M.S. Méndez ,&nbsp;M.E. Soria ,&nbsp;L. Galarza-Álvarez ,&nbsp;E. Muñoz-León ,&nbsp;D.A. Galarza","doi":"10.1016/j.anireprosci.2024.107580","DOIUrl":"10.1016/j.anireprosci.2024.107580","url":null,"abstract":"<div><p>This study aimed to assess the impact of L-<em>carnitine</em> (LC) supplementation in conventional-slow (CS) and ultra-rapid (UR) freezing media on post-thaw quality and fertilizing ability of dog epididymal spermatozoa. Sperm samples were collected from 60 epididymides obtained from 30 adult orchiectomized dogs via retrograde flushing. Twenty pooled sperm samples were then created (3 epididymal samples/pool). Four treatments were established according to the freezing method (CS and UR) and LC supplementation (5 and 0 mM [control, Co]): CS-LC5, CS-Co, UR-LC5, and UR-Co. The CS freezing involved exposing 0.25 mL straw to liquid nitrogen vapors (LN₂), while UR freezing submerged 30-µL drops of sperm samples directly into LN₂. Sperm kinematics, membrane integrity, and fertilizing ability (by heterologous in vitro fertilization using bovine oocytes) were evaluated for all treatments. Post-thaw results revealed that the CS freezing treatments resulted in significantly higher values (<em>P</em> &lt; 0.05) of curvilinear and average-path velocities, and beat-cross frequency compared to the UR freezing treatments, regardless of LC supplementation. The CS-LC5 and UR-LC5 treatments cryoprotected the sperm by increasing (<em>P</em> &lt; 0.05) the percentage of ‘live-sperm/intact-acrosome’ compared to their controls treatments CS-Co and UR-Co. Regarding fertilizing ability, the CS-LC5 treatment yielded a higher percentage (<em>P</em> &lt; 0.05) of pronuclei formation compared to both UR treatments. The UR-LC5 treatment, however, obtained greater percentage (<em>P</em> &lt; 0.05) than their control UR-Co. In conclusion, supplementation with L-<em>carnitine</em> in conventional-slow and ultra-rapid freezing improved sperm motility, plasma, and acrosome membranes integrity and fertilizing ability of dog epididymal spermatozoa.</p></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"270 ","pages":"Article 107580"},"PeriodicalIF":2.2,"publicationDate":"2024-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142098667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathological characteristics of SRY-negative 38,XX-DSD pigs: A family case report","authors":"Jinhua Wu ,&nbsp;Haiyi Yu ,&nbsp;Yuqiao Zhang ,&nbsp;Haiquan Zhao ,&nbsp;Bingzhou Zhong ,&nbsp;Congying Yu ,&nbsp;Zheng Feng ,&nbsp;Hui Yu ,&nbsp;Hua Li","doi":"10.1016/j.anireprosci.2024.107579","DOIUrl":"10.1016/j.anireprosci.2024.107579","url":null,"abstract":"<div><p>Disorders of sex development (DSD) are congenital conditions characterized by atypical development of chromosomes, gonads, or anatomical sex. XX-DSD pigs disrupt the production of high-quality breeding pigs and impede the advancement of the pig industry. However, the etiology of XX-DSD pigs remains unclear. Systematic reports on the genetic and pathological characteristics of prepubescent XX-DSD pigs in familial contexts are sparse. This study aimed to investigate the genetic and pathological features of one-month-old XX-DSD pigs within a familial context and to provide phenotypic information to elucidate the pathogenic mechanisms of XX-DSD pigs. The findings revealed that inbreeding within the XX-DSD family may contribute to the pathogenesis of XX-DSD pigs. All XX-DSD pigs in the family had a chromosomal sex of female and were male pseudohermaphrodites. The degree of masculinization of the reproductive organs varied among XX-DSD pigs, demonstrating phenotypic heterogeneity. HE staining showed that the testes of prepubescent XX-DSD pigs contained vesicles in the seminiferous tubules, with or without vestigial germ cells. Ultrastructural analyses indicated that sertoli cells, leydig cells and germ cells in the testes of XX-DSD pigs exhibited pathological damage, confirming impaired testicular function. Immunofluorescence staining revealed high expression of SRY-box transcription factor 9 (SOX9) in XX-DSD pig testicular tissues, while forkhead box L2 (FOXL2) was minimally expressed. Disordered secretion of reproductive hormones in prepubescent XX-DSD pigs indicated abnormal hypothalamic-pituitary-gonadal axis (HPGA) function. This study elucidates the genetic and pathological characteristics of prepubescent XX-DSD pigs in familial case, providing valuable insights for further exploration of the pathogenic mechanisms underlying XX-DSD.</p></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"270 ","pages":"Article 107579"},"PeriodicalIF":2.2,"publicationDate":"2024-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142076915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Can Puntius sophore breed artificially under controlled conditions? Tracing the life cycle of Puntius sophore through artificial captive breeding","authors":"Sanayaima Singha ,&nbsp;Shivendra Kumar ,&nbsp;Rajdeep Dutta ,&nbsp;Arnab Narayan Patowary ,&nbsp;Bipul Phukan ,&nbsp;Kaustubh Bhagawati ,&nbsp;Darshana Sharma ,&nbsp;Biswajyoti Bordoloi ,&nbsp;Dipak Kumar Sarma","doi":"10.1016/j.anireprosci.2024.107577","DOIUrl":"10.1016/j.anireprosci.2024.107577","url":null,"abstract":"<div><p>The development of new strategies for breeding indigenous fish species is of utmost importance in the wake of unfavorable weather events, as a result of climate change. Therefore, an attempt has been made to achieve artificial breeding of an indigenous barb, <em>Puntius sophore</em>. Two groups of juvenile fish, collected from the wild, were reared till sexual maturation. One group was reared under a natural photothermal regime and the other was reared under strictly controlled conditions with photothermal stimulation till sexual maturation and subsequently, hormonal stimulation with OVAFISH was also done for inducement of spawning. The spawning efficiencies were analyzed and the results in terms of latency period (6.74 Hrs), ovulation rate (92.2 %), fertilization rate (90.6 %), hatching rate (89.9 %), and spawning efficiency coefficient (Se) (0.828) were found better in <em>Puntius sophore</em> reared under the indoor controlled condition with photothermal manipulation and hormone administration compared to the group of fish which was reared under a natural photothermal with a hormonal stimulation. The results of this study demonstrate the captive artificial breeding of <em>Puntius sophore</em> spawners reared under a natural photothermal regime and controlled photothermal regime in indoor conditions. The outcome of the present study can be used for developing key strategies for a climate smart aquaculture for fish farmers.</p></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"268 ","pages":"Article 107577"},"PeriodicalIF":2.2,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141978314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study of the uptake mechanism of two small extracellular vesicle subtypes by granulosa cells","authors":"Xiaomei Wang ,&nbsp;Zihan Zhang ,&nbsp;Yuanmin Qi ,&nbsp;Zhimin Zhang ,&nbsp;Yixin Zhang ,&nbsp;Kai Meng ,&nbsp;Jinxiang Yuan ,&nbsp;Fusheng Quan","doi":"10.1016/j.anireprosci.2024.107576","DOIUrl":"10.1016/j.anireprosci.2024.107576","url":null,"abstract":"<div><p>As a new mechanism of intercellular communication, the uptake of extracellular vesicles (EVs) by receptor cells has become a hot topic in the field. Previously, research on the uptake of EVs has focused on the mechanism of small EVs (sEVs, also known as exosomes). As sEVs represent a mixed heterogeneous population, the issue of whether there are different uptake mechanisms for different subsets of sEVs by recipient cells urgently need to be addressed. There are EVs in follicular fluid, which play an important role in the communication between follicular cells and the development of oocytes. Previously, we isolated two subtypes of sEVs in follicular fluid: low density-sEVs (LD-sEVs) and high density-sEVs (HD-sEVs). The current study aimed to explore the uptake characteristics of these two subtypes of sEVs by granulosa cells. First, PKH67 was used to label the two sEVs subtypes, and we observed their uptake by granulosa cells using confocal microscopy and flow cytometry. We then explored the specific mechanisms underlying uptake of these two sEV subtypes by granulosa cells using specific inhibitors and RNA interference. The results showed that granulosa cells took up both kinds of sEVs through a clathrin-independent pathway. In addition to requiring caveolin, cholesterol, and Na+/H+ exchange, the uptake of HD-sEVs also depended on the activity of tyrosine kinase and phosphoinositide 3-kinase. A better understanding of the mechanism of granulosa cell uptake of different subtypes of sEVs in follicular fluid is of considerable significance leading to more accurate use of EVs for targeted treatment of infertility and other related diseases.</p></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"270 ","pages":"Article 107576"},"PeriodicalIF":2.2,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142039915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of lactose on the in vitro development of sheep secondary follicles","authors":"Kíscyla O. Andrade ,&nbsp;Alane P.O. Monte ,&nbsp;Regina L.S. Silva ,&nbsp;Ricássio S. Barberino ,&nbsp;Istefani M. Mota ,&nbsp;Gabriela C.S. Santos ,&nbsp;Valéria S. Guimarães ,&nbsp;Gizele A.L. Silva ,&nbsp;Claudener S. Teixeira ,&nbsp;Maria Helena T. Matos","doi":"10.1016/j.anireprosci.2024.107578","DOIUrl":"10.1016/j.anireprosci.2024.107578","url":null,"abstract":"<div><p>Considering that follicular development is an energy-dependent process, supplementation of the culture medium with energy substrates, such as lactose, would improve follicle viability and growth. Thus, the aim of this study was to evaluate the effect of lactose on morphology, development, glutathione (GSH) concentration, mitochondrial activity, DNA fragmentation, and meiotic resumption of oocytes from sheep secondary follicles cultured <em>in vitro</em>. Secondary follicles were isolated from the cortex of ovine ovaries and cultured individually for 18 days in α-MEM supplemented with bovine serum albumin (BSA), insulin, glutamine, hypoxanthine, transferrin, selenium and ascorbic acid (control medium: α-MEM⁺) or in α-MEM⁺ plus different concentrations of lactose (0.025, 0.05 and 0.1 M). After culture, some of the oocytes were subjected to TUNEL assay and <em>in vitro</em> maturation (IVM). Follicular morphology, glutathione (GSH) concentration and mitochondrial activity were evaluated at the end of the culture. At the day 18, the percentage of morphologically normal follicles was greater (P&lt;0.05) in the treatment of 0.025 M lactose (92.5 %) compared to the control group (75.55 %). In addition, GSH concentrations increased (P&lt;0.05) in treatment containing 0.025 M lactose compared to the other treatments. Furthermore, oocytes cultured in 0.025 M lactose had greater (<em>P</em>&lt;0.05) mitochondrial activity levels than in α-MEM+ and 0.1 M lactose. The group α-MEM⁺ presented a increase of TUNEL-positive oocytes (35.09 %) compared to 0.025 lactose (9.09 %). The percentage of meiotic resumption was greater (P&lt;0.05) in oocytes from secondary follicles cultured in 0.025 M lactose (54.5 %) than in α-MEM⁺ (45.5 %). In conclusion, 0.025 M lactose improved survival, GSH and active mitochondria levels and meiotic resumption of oocytes from <em>in vitro</em> cultured secondary follicles. Supplementation of the culture medium of preantral follicles with lactose can gradually provide energy to follicular cells, potentially enhancing the production of viable oocytes for biotechniques such as IVM and in vitro fertilization.</p></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"270 ","pages":"Article 107578"},"PeriodicalIF":2.2,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142098666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}