Mariana C. Fabra, Anabella A. Campagna, Nicolás Farnetano, Juan P. Anchordoquy, Juan M. Anchordoquy, Ana C. Carranza-Martin, Cecilia C. Furnus, Noelia Nikoloff
{"title":"Alpha-Lipoic acid supplementation during in vitro culture of abattoir- and OPU-derived bovine oocytes improves embryonic quality","authors":"Mariana C. Fabra, Anabella A. Campagna, Nicolás Farnetano, Juan P. Anchordoquy, Juan M. Anchordoquy, Ana C. Carranza-Martin, Cecilia C. Furnus, Noelia Nikoloff","doi":"10.1016/j.anireprosci.2024.107667","DOIUrl":"10.1016/j.anireprosci.2024.107667","url":null,"abstract":"<div><div>The Ovum Pick-Up (OPU) technique offers a rapid path to genetic improvement. Embryonic genome activation (EGA) is crucial for successful embryonic development and occurs in two phases: minor EGA (MN<sub>EGA</sub>) from 2-cell to 8-cell stages, and major EGA (MJ<sub>EGA</sub>) from 8-cell to 16-cell stages. Supplementation with alpha-lipoic acid (ALA) during MN<sub>EGA</sub> using abattoir derived-oocytes has been shown to enhance <em>in vitro</em> embryo production. Two experiments were carried out in the study at hand. Experiment I aimed to evaluate ALA effects on embryo development and quality during MJ<sub>EGA</sub> using abattoir-derived oocytes. Oocytes were treated with either a Control <em>in vitro</em> culture (IVC) medium or 2.5 µM ALA during MJ<sub>EGA</sub>. The ALA presented a trend to increase the blastocyst cell number on day 7 (<em>P</em> = 0.06), along with reducing ROS levels and increasing GSH levels (<em>P</em> ≤ 0.05). Experiment II aimed to verify whether the results from abattoir- derived oocytes could be extrapolated to OPU-derived oocytes. The treatments included a Control, 2.5 µM ALA during MN<sub>EGA</sub>, and 2.5 µM ALA during MJ<sub>EGA</sub>. The ALA supplementation during MJ<sub>EGA</sub> improved the total blastocyst rate on day 8 (<em>P</em> = 0.04), with a trend towards increased blastocyst cell number on day 7 (<em>P</em> = 0.09). No improvements were observed in MN<sub>EGA</sub>. Overall, ALA supplementation during MJ<sub>EGA</sub> is more effective than during MN<sub>EGA</sub> for improving the embryonic quality of OPU-derived oocytes.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"272 ","pages":"Article 107667"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142806045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jessica Plaza , Gustavo Olivieri , Marcelo Miragaya
{"title":"Effect of the addition of EDTA to Kenney extender for donkey semen cooling","authors":"Jessica Plaza , Gustavo Olivieri , Marcelo Miragaya","doi":"10.1016/j.anireprosci.2024.107731","DOIUrl":"10.1016/j.anireprosci.2024.107731","url":null,"abstract":"","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"272 ","pages":"Article 107731"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143289843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Eduardo Alves Lima , Laís Reis Carvalho , Raphael Evangelista Orlandi , Luiz Manoel Souza Simões , Miguel Pizzolante Bottino , Ana Paula Castro Santos , Fernando de Oliveira Scarpa , Jose Nelio de Sousa Sales
{"title":"Effect of maternal heat stress at different stages of pregnancy on the reproductive performance and antral follicle count of the progeny of Holstein cows","authors":"Eduardo Alves Lima , Laís Reis Carvalho , Raphael Evangelista Orlandi , Luiz Manoel Souza Simões , Miguel Pizzolante Bottino , Ana Paula Castro Santos , Fernando de Oliveira Scarpa , Jose Nelio de Sousa Sales","doi":"10.1016/j.anireprosci.2024.107665","DOIUrl":"10.1016/j.anireprosci.2024.107665","url":null,"abstract":"<div><div>The objective was to evaluate the effect of maternal exposure to heat stress conditions at different stages of pregnancy on the reproductive performance and antral follicle count of progeny. The study was conducted at five commercial dairy farms located in southeastern Brazil. Retrospective data (2002 and 2015) on reproductive indices of daughters of Holstein cows that were exposed to heat stress conditions in the first (<em>n</em> = 447), second (n = 729) or third (<em>n</em> = 746) trimester of pregnancy and daughters of cows not exposed to heat stress (<em>n</em> = 359) were analyzed. Antral follicle count (AFC) and ovarian size were determined in a subgroup of animals (<em>n</em> = 242) by transrectal ultrasound. Statistical analysis was performed using the SAS GLIMMIX procedure. The number of services after first calving was lower in daughters of cows not exposed to heat stress conditions than in those exposed during different trimesters of pregnancy (<em>P</em> = 0.03). The calving interval was longer for daughters of cows exposed to heat stress conditions in the second and third trimesters of pregnancy than for those not exposed to heat stress (<em>P</em> = 0.01). In addition, the probability of pregnancy at first AI, (<em>P</em> = 0.01) and the AFC (<em>P</em> = 0.001) were greater in daughters of cows not exposed to heat stress conditions than in those exposed during the different phases of the gestational period. These findings indicate that maternal heat stress in different stages of pregnancy negatively affect the future fertility and AFC of bovine offspring.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"272 ","pages":"Article 107665"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142817025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The effect of paternal age on progeny performance in Australian Thoroughbred horses","authors":"Ceilidh Jenkins , Roisin Griffin , Aleona Swegen , Natasha Hamilton , Zamira Gibb","doi":"10.1016/j.anireprosci.2024.107737","DOIUrl":"10.1016/j.anireprosci.2024.107737","url":null,"abstract":"","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"272 ","pages":"Article 107737"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143280786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rodrigo Mezêncio Godinho , Ina Andersen-Ranberg , Ann Helen Gaustad , Carolina Lima Alvares da Silva , Eli Grindflek , Louisa Jane Zak
{"title":"Fine-tuning the screening of young boars for production centers of artificial insemination semen doses","authors":"Rodrigo Mezêncio Godinho , Ina Andersen-Ranberg , Ann Helen Gaustad , Carolina Lima Alvares da Silva , Eli Grindflek , Louisa Jane Zak","doi":"10.1016/j.anireprosci.2024.107727","DOIUrl":"10.1016/j.anireprosci.2024.107727","url":null,"abstract":"","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"272 ","pages":"Article 107727"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143289244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}