Animal Reproduction Science最新文献

{"title":"In vitro 2D and 3D culture of frozen adult ovine testicular fragments","authors":"Francisco Denilson Rodrigues Gomes , Rensson Homero Céliz Ygnacio , Lucy Vanessa Sulca Ñaupas , Gildas Mbemya Tetaping , Ana Paula Robeiro Rodrigues","doi":"10.1016/j.anireprosci.2024.107718","DOIUrl":"10.1016/j.anireprosci.2024.107718","url":null,"abstract":"","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"272 ","pages":"Article 107718"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143272309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of two sperm selection devices using variable sperm loads to enhance quality and sperm harvest from fresh-diluted stallion ejaculates","authors":"Márcio Menezes Nunes , Maria Luiza Munhoz , Frederico Augusto de Souza Freitas , Maria Florencia Gallelli , Gustavo Ferrer Carneiro , Marcelo Miragaya","doi":"10.1016/j.anireprosci.2024.107729","DOIUrl":"10.1016/j.anireprosci.2024.107729","url":null,"abstract":"","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"272 ","pages":"Article 107729"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143277740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Animal- and plant-based extenders for turkey semen stored at 5oC","authors":"Balogun Adedeji Suleimon , Akinosun Akintunde Akinbola, Lawal Ibilola Azeezat, Ojetunde Taiwo Hazzan, Hammed Barakat Bisola","doi":"10.1016/j.anireprosci.2024.107742","DOIUrl":"10.1016/j.anireprosci.2024.107742","url":null,"abstract":"","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"272 ","pages":"Article 107742"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143289171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gonadal expression profiles reveal the underlying mechanisms of temperature effects on sex determination in the large-scale loach (Paramisgurnus dabryanus)","authors":"Lei Zhang ,&nbsp;Yutong Fang ,&nbsp;Mijuan Shi ,&nbsp;Keyi Ren ,&nbsp;Xin Guan ,&nbsp;Waqar Younas ,&nbsp;Yingyin Cheng ,&nbsp;Wanting Zhang ,&nbsp;Yaping Wang ,&nbsp;Xiao-Qin Xia","doi":"10.1016/j.anireprosci.2024.107661","DOIUrl":"10.1016/j.anireprosci.2024.107661","url":null,"abstract":"<div><div>The sex determination mechanism in large-scale loach (<em>Paramisgurnus dabryanus</em>) follows a ZZ/ZW system, with sexual differentiation regulated by both genotypic factors and temperature effects (GSD+TSD), where elevated temperatures result in a higher proportion of males. Currently, research on the sex determination mechanisms in large-scale loach is limited, and the specific gene expression profiles and the role of temperature in influencing sex remain largely unknown. This study investigated the impact of temperature on the sex ratio in cultured populations of the large-scale loach, and then identified a female-specific genetic marker by whole genome sequencing, facilitating the distinguishing of females, males, and pseudo-males within this population. Transcriptomic analysis was subsequently performed on these groups, and the data revealed a similar expression pattern between pseudo-males and true-males. The research combined differential expression analysis with WGCNA to construct a regulatory network of nine sex differentiation-related genes (SDG) (<em>map3k4</em>, <em>trpv4</em>, <em>hsd17b12a</em>, <em>wt1</em>, <em>ar</em>, <em>dmrt1</em>, <em>bcar1</em>, <em>sox9a</em>, <em>cyp17a1</em>), indicating that sex differentiation in large-scale loach is probably driven by the regulation of male-related genes. The transcriptomic analysis suggested that temperature significantly modified the expression of SDG in the ovaries, while in the testes, it predominantly affects metabolism-related pathways. We established a temperature-sensitive gene network in females, based on the correlation between gene expression and temperature, as well as the number of co-regulated genes in female data. We propose that, with increasing temperature, <em>wt1</em> serves as a central regulator, leading to the down-regulation of <em>foxl2a</em>, <em>cyp19a1a,</em> and the cholesterol biosynthesis-related gene <em>sqlea</em>, ultimately resulting in the development of pseudo-males.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"272 ","pages":"Article 107661"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142791012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pre-conception nutrition estimated from milk composition characterises the sex ratio of offspring in cattle","authors":"Janez Belšak ,&nbsp;Janez Jeretina ,&nbsp;Maja Prevolnik Povše ,&nbsp;Dejan Škorjanc ,&nbsp;Janko Skok","doi":"10.1016/j.anireprosci.2024.107650","DOIUrl":"10.1016/j.anireprosci.2024.107650","url":null,"abstract":"<div><div>In livestock breeding, offspring of a particular sex are often favoured. Various biological mechanisms influence the offspring sex ratio (OSR). It has been hypothesised that maternal body condition/nutritional status influence OSR. We analysed the relationship between the nutritional status of the cow and OSR. The analysed dataset contained 40,913 milk recordings of 39,192 cows from 3625 farms. Nutritional status was estimated based on the fat-to-protein (F:P) ratio and urea content in milk in the seven to fourteen days prior to conception. Somatic cell count (SCC) was also considered. Cows with &gt; 150,000 SSC were analysed separately. When SSC was normal (≤150,000), high urea with high F:P resulted in a higher probability of male offspring (61 %), with OSR significantly deviating towards male (1.47). Conversely, a high urea level and low F:P resulted in a higher probability of female offspring (57 %), with OSR significantly in favour of females (0.79). At normal or low milk urea, the sex of the offspring did not change within F:P classes. The sex ratio did not deviate from 1:1 when F:P and urea were in the normal range. When SCC was high, the probability of particular sex did not change significantly in either combination of F:P and urea, but OSR showed a tendency toward males (OSR&gt;1) when F:P and urea were low or normal. Our results confirmed the influence of maternal nutritional status on the offspring sex. Thus, adjusting nutrition in the pre-insemination period seems to be a way to influence the sex ratio of offspring in dairy cows.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"272 ","pages":"Article 107650"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142791014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effect of epidermal growth factor on cryopreservation of dromedary camel epididymal spermatozoa: Evidence from in vitro and in silico studies","authors":"Sameh A. Abdelnour ,&nbsp;Wael A. Khalil ,&nbsp;Mahmoud A.E. Hassan ,&nbsp;Ibrahim T. El-Ratel ,&nbsp;Mostafa A. El-Harairy ,&nbsp;Sherif M. Dessouki ,&nbsp;Kandil A.A. Attia","doi":"10.1016/j.anireprosci.2024.107662","DOIUrl":"10.1016/j.anireprosci.2024.107662","url":null,"abstract":"<div><div>Epidermal growth factor (EGF) plays a crucial role in maintaining male reproductive capacity in mammals, however, its protective effects on cryopreserved dromedary camel epididymal spermatozoa have not been thoroughly investigated. This study aims to investigate the potential protective role of EGF on cryopreserved camel epididymal spermatozoa, supported by evidence from a molecular docking study. We assessed sperm motility, kinematics parameters, oxidative stress, ultrastructural changes, apoptosis, and molecular docking markers in camel epididymal spermatozoa following cryopreservation. Camel epididymal spermatozoa (n = 30 pairs of testes) were collected from local slaughterhouses. The epididymal spermatozoa were diluted with a freezing medium (SHOTOR extender) supplemented with different concentrations of EGF; 0 (EGF0), 50 (EGF50), 100 (EGF100), 200 (EGF200), and 400 (EGF400) ng/mL in SHOTOR extender and cryopreserved using a standard protocol. All EGF groups showed significant improvements in sperm progressive motility, viability, and sperm membrane function after equilibration at 5 °C for 24 hours. Regarding frozen-thawed samples, sperm progressive motility and some kinematic parameters (DAP, VSL, VCL and AHL) were significantly higher in the EFG400 group compared to the other groups (<em>P</em> &lt; 0.01). A significant increase in the percentage of live/acrosome-intact sperm was observed, accompanied by a significant decrease in malondialdehyde levels in all EGF groups (<em>P</em> &lt; 0.05). Both the EGF200 and EGF400 groups showed significantly higher sperm viability and significantly lower percentages of apoptotic and necrotic sperm compared to the other groups (<em>P</em> &lt; 0.05). EGF supplementation preserved the ultrastructural integrity and cryotolerance of epididymal camel spermatozoa. The docking analysis indicated that EGF exhibited higher binding affinity with apoptosis sperm markers, including caspase-3 and bcl-2-associated X (Bax) proteins, with binding energies of −502.0 and −621.0 kcal/mol, respectively. In conclusion, the addition of EGF to SHOTOR extender was found to have beneficial effects on sperm motility, kinematics parameters, sperm viability, acrosome integrity, sperm ultrastructural features, and reduced oxidative stress and apoptosis-like changes in cryopreserved epididymal camel spermatozoa.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"272 ","pages":"Article 107662"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142791016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological, morphometric and ultrastructural characterization of epididymal spermatozoa from red-nosed rats (Wiedomys pyrrhorhinos)","authors":"Pedro Augusto Pinheiro Brito,&nbsp;Yuri Gonçalves Matos,&nbsp;Náyra Rachel Nascimento Luz,&nbsp;Gabriel Santos Costa Bezerra,&nbsp;Andréia Maria Silva,&nbsp;Alexandre Rodrigues Silva","doi":"10.1016/j.anireprosci.2024.107750","DOIUrl":"10.1016/j.anireprosci.2024.107750","url":null,"abstract":"","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"272 ","pages":"Article 107750"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143286677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenazine ethosulfate alters the kinematic parameters of boar sperm","authors":"Janine de Camargo ,&nbsp;José Victor Braga ,&nbsp;Jordan Grey ,&nbsp;Pablo Amador ,&nbsp;Ágape Helbing ,&nbsp;Thomaz Lucia Jr ,&nbsp;Rafael Mondadori ,&nbsp;Renata Simões ,&nbsp;José Pandolfi ,&nbsp;Mariana Marques","doi":"10.1016/j.anireprosci.2024.107722","DOIUrl":"10.1016/j.anireprosci.2024.107722","url":null,"abstract":"","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"272 ","pages":"Article 107722"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143352961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Strategies to increase circulating progesterone in late metestrus and early diestrus in recipient dairy heifers and cows: A focus on induction of accessory corpus luteum formation","authors":"Marcelo S. El Azzi ,&nbsp;João Paulo N. Martins ,&nbsp;José C. de Souza","doi":"10.1016/j.anireprosci.2024.107652","DOIUrl":"10.1016/j.anireprosci.2024.107652","url":null,"abstract":"<div><div>The capacity of recipients to support transferred embryos is a challenge for dairy cattle production systems. This review explores methods for increasing P4 to improve early pregnancy establishment in dairy cattle. A key aspect of P4 supplementation during the critical first weeks of pregnancy is its correlation with successful embryo elongation. Moreover, P4 was correlated to an increased chance of embryo survival after maternal recognition of pregnancy. Internal releasing devices increase circulating P4 concentrations but may negatively impact fertility possibly due to unwanted health pathologies such as vaginitis and the still undefined mechanism of haste luteolysis. The accessory corpus luteum (CL) induction in recipient dairy cattle increases endogenous P4 output increasing circulating P4 concentrations. Two ovulatory inducers are commonly used: gonadotropin-releasing hormone (GnRH) and human chorionic gonadotropin (hCG). Despite their distinct structures, GnRH and hCG are equally capable of inducing ovulation in cattle. The P4 supplementation by an accessory CL formation was widely used in fixed-time artificial insemination breeding programs, but still not exhaustively studied in embryo transfer (ET) programs. The induction of an accessory CL in ET programs has yielded inconsistent results depending on parity, embryo type production, embryo stage of development, and type of hormone used. Although maternal physiological mechanisms support the idea of increasing P4 for higher fertility and better embryo development, the actual results in creating a high-P4 maternal environment are still uncertain.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"272 ","pages":"Article 107652"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142791019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnosing american bison pregnancy during its two last trimesters: Comparison between rectal palpation associated with ultrasonography and hormonal assays in serum or in feces","authors":"Vincent Frisée ,&nbsp;Goulven Rigaux ,&nbsp;Patrice Dufour ,&nbsp;Flore Brutinel ,&nbsp;Philippe Bossaert ,&nbsp;Sophie Egyptien ,&nbsp;Olimpia Barbato ,&nbsp;Frédéric Farnir ,&nbsp;Caroline Le Goff ,&nbsp;Stéfan Deleuze ,&nbsp;Etienne Cavalier ,&nbsp;Jérôme Ponthier","doi":"10.1016/j.anireprosci.2024.107660","DOIUrl":"10.1016/j.anireprosci.2024.107660","url":null,"abstract":"<div><div>The study aimed to propose sensitive, specific and easy pregnancy diagnosis for American Bison. Trans-rectal Palpation and Ultrasonography (TRPUS), concentration of Pregnancy Associated Glycoproteins (PAG), Progesterone (P4), Estrone (E1), Estrone-Sulfate (E1S) in serum and P4 and E1S in feces were compared. Immunoassay was used for PAG, whereas steroids were assayed using Liquid Chromatography coupled to Mass Spectrometry. Pregnancy and its period were retrospectively assessed after calving. Animals included were distributed as follows: 40 were non-pregnant, 27 were in their second and 15 in their third trimester of gestation. <em>Fisher’s</em> test was used to determine sensitivity and specificity of TRPUS. Thresholds leading to best sensitivities and specificities for hormonal assays were determined using Receiver Operating Characteristic Curves. Although TRPUS requires contention, palpation skills, it was conclusive in 70.63 % of cases and good sensitivity (97.50 %) and decent specificity (83.33 %) were observed. Concentrations of PAG, P4, E1S and E1 in serum were higher in Pregnant animals (P), with thresholds giving decent specificity and sensitivity (lower sensitivity for E1 and E1S: 92.11 %; lower specificity for P4: 93.48 %). In feces, P4 and E1S were higher in P: pregnancy diagnosis using E1S assay in feces showed 100 % of sensitivity and specificity. In conclusion, assaying E1S in feces during the two last trimesters of American Bisons pregnancy is a sensitive and specific gestation diagnosis. Pregnancy diagnosis using TRPUS can be useful in ranches but requires contention and skill adaptation. Serum hormonal assays can help evaluating the pregnancy period but also require contention.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"272 ","pages":"Article 107660"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142783872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}