一项代谢组学研究揭示了杜洛克猪精液中的关键氨基酸和胺作为性成熟的生物标志物

IF 2.2 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science Pub Date : 2025-02-20 DOI:10.1016/j.anireprosci.2025.107800

Asmita Shrestha , Ann Helen Gaustad , Janne Beate Øiaas , Elisabeth Kommisrud , Maren van Son , Anna Nordborg , Anne Hege Alm-Kristiansen

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引用次数: 0

摘要

与性成熟相关的猪精液代谢组学分析对于改善生育管理和育种至关重要，氨基酸和胺在生殖过程中起着关键作用。本研究旨在探讨猪精子和精浆中氨基酸和胺在青春期至性成熟期间的变化，并寻找性成熟的潜在生物标志物。在大约7个月（1岁）、8.5个月（2岁）和10个月（3岁）的时间里，从相同的15头杜洛克公猪身上收集精液。采用液相色谱-质谱法分别分析精子和精浆中的氨基酸和胺。多变量分析（PLS-DA）显示，在1岁至3岁之间，精子中氨基酸和胺的年龄依赖性变化明显，而精浆中的变化则更为微妙。单因素分析（重复测量ANOVA/Friedman）显示，谷氨酸和牛磺酸在精浆中具有显著的两两差异（P <； 0.05）。精子中15种氨基酸（谷氨酸、丙氨酸、天冬氨酸、胆碱、牛磺酸、组氨酸、蛋氨酸、色氨酸、亮氨酸、胱氨酸、酪氨酸、精氨酸、赖氨酸、缬氨酸和甘氨酸）呈显著的两两差异（P <； 0.05）。VIP评分（>1.5）优先考虑谷氨酸、丙氨酸、天冬氨酸和胆碱，它们是与性成熟相关的谷氨酸、天冬氨酸和谷氨酸代谢和组氨酸代谢相关的变异和途径分析的关键因素。我们的研究强调了性成熟过程中的代谢变化，确定了评估生殖成熟度的潜在生物标志物。这些发现是朝着优化幼公猪在育种中的使用、提高遗传增益和降低与它们在人工智能中心非生产性日子相关的成本迈出的初步步骤。

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A metabolomic study uncovering key amino acids and amines in Duroc boar semen as biomarkers of sexual maturity

Metabolomic analysis of boar semen associated with sexual maturation is essential for improving fertility management and breeding, with amino acids and amines playing key roles in the reproductive process. This study aimed to explore changes in amino acids and amines in boar spermatozoa and seminal plasma during puberty to sexual maturity and identify potential biomarkers of sexual maturity. Semen was collected from the same 15 Duroc boars over time at approximately 7 months (Age 1), 8.5 months (Age 2), and 10 months (Age 3). Liquid chromatography-mass spectrometry was used to analyse amino acids and amines in spermatozoa and seminal plasma separately. Multivariate analysis (PLS-DA) revealed pronounced age-dependent changes in amino acids and amines in spermatozoa between Age 1 and Age 3, and more subtle shifts in seminal plasma. Univariate analysis (Repeated measure ANOVA/Friedman) revealed that glutamate and taurine had significant pairwise differences in seminal plasma (P < 0.05). In sperm, 15 amino acids (glutamate, alanine, aspartate, choline, taurine, histidine, methionine, tryptophan, leucine, cystine, tyrosine, arginine, lysine, valine and glycine) exhibited significant pairwise differences (P < 0.05). VIP scoring (>1.5) prioritised glutamate, alanine, aspartate, and choline as key contributors to the variations and pathway analysis implicated alanine, aspartate and glutamate metabolism, and histidine metabolism linked to sexual maturity. Our study highlights metabolic changes during sexual maturation, identifying potential biomarkers for assessing reproductive maturity. These findings are initial steps toward optimising younger boars' usage in breeding, enhancing genetic gain, and reducing costs associated with their non-productive days at AI centres.

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来源期刊

Animal Reproduction Science 农林科学-奶制品与动物科学

CiteScore

4.50

自引率

9.10%

发文量

136

审稿时长

54 days

期刊介绍： Animal Reproduction Science publishes results from studies relating to reproduction and fertility in animals. This includes both fundamental research and applied studies, including management practices that increase our understanding of the biology and manipulation of reproduction. Manuscripts should go into depth in the mechanisms involved in the research reported, rather than a give a mere description of findings. The focus is on animals that are useful to humans including food- and fibre-producing; companion/recreational; captive; and endangered species including zoo animals, but excluding laboratory animals unless the results of the study provide new information that impacts the basic understanding of the biology or manipulation of reproduction. The journal''s scope includes the study of reproductive physiology and endocrinology, reproductive cycles, natural and artificial control of reproduction, preservation and use of gametes and embryos, pregnancy and parturition, infertility and sterility, diagnostic and therapeutic techniques. The Editorial Board of Animal Reproduction Science has decided not to publish papers in which there is an exclusive examination of the in vitro development of oocytes and embryos; however, there will be consideration of papers that include in vitro studies where the source of the oocytes and/or development of the embryos beyond the blastocyst stage is part of the experimental design.