Aquaculture Reports最新文献

{"title":"Whole-genome sequencing and resequencing for genome-wide identification of candidate sex-specific markers in the camouflage grouper (Epinephelus polyphekadion)","authors":"Shoujia Jiang ,&nbsp;Yaorong Wang ,&nbsp;Jin Zhang ,&nbsp;Zhiqiang Ruan ,&nbsp;Chao Bian ,&nbsp;Jiaqi Lin ,&nbsp;Shujia Liao ,&nbsp;Kejun Chen ,&nbsp;Yuqing Yang ,&nbsp;Jinhui Wu ,&nbsp;Xinxin You ,&nbsp;Cheng Peng ,&nbsp;Shufang Cui ,&nbsp;Haoran Lin ,&nbsp;Qiong Shi ,&nbsp;Shuisheng Li ,&nbsp;Yong Zhang","doi":"10.1016/j.aqrep.2025.103020","DOIUrl":"10.1016/j.aqrep.2025.103020","url":null,"abstract":"<div><div>As an endangered reef fish, the camouflage grouper (<em>Epinephelus polyphekadion</em>) has been recently cultured in south China costal area. The prevalent utilization of males <em>E. polyphekadion</em> in distant hybridization highlights the economic and ecological importance of developing genetic methods for accurate sex identification at early developmental stages. Here, we present a 1.08-Gb chromosome-level genome assembly for <em>E</em>. <em>polyphekadion</em> by using Illumina short-read, PacBio long-read, and Hi-C sequencing technologies. Remarkably, 99.69 % of the scaffolds were anchored onto 24 chromosomes, with contig N50 and scaffold N50 values of 28.09 Mb and 45.93 Mb, respectively. The genome comprises 42.85 % repetitive sequences and is annotated with 24,067 protein-coding genes, achieving a 98.37 % functional annotation rate. BUSCO analysis confirmed 98.0 % completeness, attesting to the assembly's high quality. Importantly, through whole-genome resequencing, we identified two sex-specific markers (three SNP sites), enabling a PCR-based gender identification assay for <em>E</em>. <em>polyphekadion</em>. Comparative genomic analyses with other Perciformes reveal Epinephelinae evolutionary trajectories across chromosomes. This study not only advances our understanding of the genetic and evolutionary dynamics of <em>E. polyphekadion</em> but also provides a crucial resource for genetic breeding and studies on gonadal development in reef fishes.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"44 ","pages":"Article 103020"},"PeriodicalIF":3.7,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144749559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary enzyme-hydrolyzed marine proteins supplementation promotes appetite, enhances lipid and protein metabolism in Litopenaeus vannamei fed reduced fish meal diets","authors":"Xiaoyue Li ,&nbsp;Tiantian Xu ,&nbsp;Yu He ,&nbsp;Min Jin ,&nbsp;Yinzhao Zhang ,&nbsp;Feng Tang ,&nbsp;Qicun Zhou","doi":"10.1016/j.aqrep.2025.103026","DOIUrl":"10.1016/j.aqrep.2025.103026","url":null,"abstract":"<div><div>An 8-weeks feeding trial was conducted to evaluate the effect of dietary enzyme-hydrolyzed marine protein (EMP) supplementation on appetite, protein and lipid metabolism of <em>Litopenaeus vannamei</em> fed reduced fishmeal diets. The control group was supplemented with 30 % fishmeal, in the experimental group, 28 % fish meal was supplemented with 1 % EMP (1 % EMP), and 26 % fish meal with 2 % EMP (2 % EMP). The results showed that there were no significant differences in growth performance in each group (<em>P</em> &gt; 0.05). Shrimp in control group exhibited higher total cholesterol, triglyceride and low-density lipoprotein cholesterol in hemolymph than those in the other groups (<em>P</em> &lt; 0.05). The results of intestinal histology showed that shrimp fed with 1 % EMP diet exhibited higher fold height and fold width than those fed the other diets (<em>P</em> &lt; 0.001). Compared with control group, the lipid catabolism genes and regulatory factors (<em>atgl, hsl, ampkα</em>, <em>ampkγ</em>, <em>acox1</em>) was significantly enhanced in 1 % EMP group (<em>P</em> &lt; 0.05). Moreover, the neurotransmitter and appetite related-genes expressions (such as <em>oa-ta</em>, <em>tdc</em>, <em>tbh</em> and <em>ghs-r1</em>) in 1 % EMP group of cerebral ganglion were significantly up-regulated than those in other group (<em>P</em> &lt; 0.05). Dietary 1 % EMP supplementation activated expressions of TORC1 pathway-related genes (<em>tor</em>, <em>rheb</em>, <em>slc3a2</em>, <em>4e-bp</em>) in the hepatopancreas than those feed the control diet (<em>P</em> &lt; 0.05). In conclusion, the results revealed that reducing 2 % fish meal and adding 1 % EMP in the diet could improve the appetite, lipid and protein metabolism, intestinal and hepatopancreas health of juvenile Pacific white shrimp.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"44 ","pages":"Article 103026"},"PeriodicalIF":3.7,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144738197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glutamate and serotonin enhance feeding performance, digestive enzyme activity, and immunity in the female giant freshwater prawn, Macrobrachium rosenbergii","authors":"Thiraphon Deethaisong ,&nbsp;Warinthip Vetkama ,&nbsp;Pannasa Manjit ,&nbsp;Ruchanok Tinikul ,&nbsp;Prasert Sobhon ,&nbsp;Yotsawan Tinikul","doi":"10.1016/j.aqrep.2025.103002","DOIUrl":"10.1016/j.aqrep.2025.103002","url":null,"abstract":"<div><div>This study investigated the presence of glutamate (Glu) and serotonin (5-HT) in the digestive organs and their effects on feeding performance, digestive enzyme activity, and immunity throughout the ovarian cycle in female giant freshwater prawns (<em>Macrobrachium rosenbergii</em>). By high-performance liquid chromatography (HPLC), Glu and 5-HT were detected in the foregut, midgut, hindgut, and hepatopancreas, at the highest concentrations in the late ovarian stage and the lowest concentrations in the early ovarian stage. Through immunohistochemistry, the presence of Glu-immunoreactivity (-ir) and 5-HT-ir were observed in all digestive organs, and their intensities were shown to correlate with the levels detected by those observed via HPLC. Prawns injected with Glu and 5-HT at the doses of 2.5 × 10⁻⁶ and 2.5 × 10⁻⁷ mol/prawn, markedly increased food consumption, gastric emptying, and midgut index as well as enhanced amylase, protease, and lipase activities, compared with the control group (<em>P</em> &lt; 0.05). Glu and 5-HT also enhanced epithelial cell height, villi height, and crypt depth and promoted the reactivity of PAS/AB in the digestive organs. In addition, treatment with Glu or 5-HT significantly upregulated <em>M. rosenbergii</em> NPF (<em>MrNPF)</em> and <em>immune-related</em> transcripts (<em>P</em> &lt; 0.05). Collectively, these findings demonstrated the promising effects of Glu and 5-HT at a dose of 2.5 × 10⁻⁶ mol/prawn in stimulating feeding, digestive activities, and immunity, which could potentially be used to enhance feeding, growth, and maintain the health status of female prawn in aquaculture.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"44 ","pages":"Article 103002"},"PeriodicalIF":3.7,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144749558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of a multi-epitope vaccine for Nocardia seriolae in Micropterus salmoides","authors":"Yingbing Su ,&nbsp;Xiaoman Qin ,&nbsp;Lin Zhu,&nbsp;Feng Liu","doi":"10.1016/j.aqrep.2025.103000","DOIUrl":"10.1016/j.aqrep.2025.103000","url":null,"abstract":"<div><div><em>Nocardia seriolae</em> can cause chronic granulomatous bacterial disease in fish, resulting in tissue necrosis. Currently, there is no effective method to control <em>N. seriolae</em> infection, which has led to significant economic losses in the aquaculture industry. In this study, immunoinformatics tools were employed to predict antigenic epitopes of seven antigenic proteins of <em>N. seriolae</em>: DnaK, GroEL, RpsA, PspA, TerD, RplL, and Ag85L. A total of 21 B-cell epitopes and 21 T-cell epitopes were predicted and concatenated into a multi-epitope protein named NSEP. To evaluate its immunogenicity, NSEP was used to immunize <em>Miropterus salmoides</em>. Four weeks post-immunization, compared with the adjuvant group, significant increases were observed in the serum activities of lysozyme (LYZ), acid phosphatase (ACP), alkaline phosphatase (AKP), and superoxide dismutase (SOD). Moreover, the transcription levels of immune related genes <em>IL-10</em>, <em>IL-1 β</em>, <em>TNF-α</em>, <em>IFN-γ</em>, <em>IgM</em>, and <em>MHCI-α</em> were significantly upregulated in both liver and spleen tissues. Following challenge with <em>N. seriolae</em> NS01, the NSEP-immunized group exhibited a relative percentage survival (RPS) of 61.29 %, along with significantly reduced bacterial loads. These results demonstrated that the multi-epitope protein NSEP holds promise as a candidate subunit vaccine against <em>N. seriolae</em> infection in <em>M. salmoides</em>.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"44 ","pages":"Article 103000"},"PeriodicalIF":3.7,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144738196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Estimation of genetic parameters for the alkalinity tolerance trait in Urechis unicinctus","authors":"Wei Zhang ,&nbsp;Juan Sui ,&nbsp;Kai Wang ,&nbsp;Mingyu Shao ,&nbsp;Yongzheng Zhao ,&nbsp;Hongtao Wang ,&nbsp;Zongtao Chen ,&nbsp;Zhifeng Zhang ,&nbsp;Yubin Ma","doi":"10.1016/j.aqrep.2025.103018","DOIUrl":"10.1016/j.aqrep.2025.103018","url":null,"abstract":"<div><div>The echiura worm <em>Urechis unicinctus</em> has high nutritional and medicinal values and has been identified as a Chinese featured aquatic germplasm resource. However, overfishing and social demand have made it difficult to fill this market gap. The vast area of saline-alkali water in China remains a huge unexploited resource owing to its harsh environmental conditions. To address the scarcity of alkali-tolerant germplasm resources of <em>U. unicinctus</em> in China, we established 15 full-sib families derived from wild Qinhuangdao populations for systematic alkali stress experiments. The 72-h LC50 of <em>U. unicinctus</em> was determined to be 85.59 mM. We estimated the genetic parameters of the alkalinity tolerance trait in <em>U. unicinctus</em> for the first time and calculated the heritability of the alkalinity tolerance trait as 0.14 ± 0.06 (<em>P</em> &lt; 0.05), which belongs to a low level of heritability, indicating that it can be improved by population hybridization, molecular breeding, and family selection breeding. In addition, the genetic and phenotypic correlation coefficients between the alkalinity tolerance trait and body weight were 0.97 ± 0.02 and 0.82 ± 0.02 (<em>P</em> &lt; 0.01), respectively, with a Pearson correlation coefficient of 0.49 (<em>P</em> &lt; 0.01). These results indicate that alkali tolerance is significantly correlated with body weight and that alkali tolerance can be selected indirectly through body weight. The results of this study provide valuable insights for the selection breeding of superior varieties with alkali tolerance in <em>U. unicinctus</em> and have important value in promoting the utilization of saline-alkali water resources.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"44 ","pages":"Article 103018"},"PeriodicalIF":3.7,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144738198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioconversion of aquaculture waste blended with vegetable by-products using Hermetia illucens larvae: Process parameters and larval quality","authors":"Ivã Guidini Lopes ,&nbsp;Viktoria Wiklicky ,&nbsp;Cecilia Lalander","doi":"10.1016/j.aqrep.2025.102961","DOIUrl":"10.1016/j.aqrep.2025.102961","url":null,"abstract":"<div><div>The constantly increasing aquaculture production generates high amounts of biowaste worldwide, which must be properly treated in order to keep aquaculture’s footprint low. This study aimed at evaluating the bioconversion of aquaculture waste streams (RAS sludge, fish trimmings, and harvest macroalgae waste) using black soldier fly (<em>Hermetia illucens</em>, BSF) larvae and the quality of the resulting larval biomass. The study was conducted in a modified shipping container simulating a large-scale setting and the diets were formulated using brewery spent grains and cabbage as base, mixing in aquaculture waste, aiming to treat the highest inclusion rate possible. A bioconversion efficiency of above 20 %_DM and a material reduction generally above 55 %_DM were observed, while generating a larval biomass that was rich in protein (&gt; 35 %_DM), essential amino acids and fatty acids. The larvae reared on fish trimmings (from an anchovy processing plant) had the highest crude fat content (29.0 ± 1.1 %_DM) in relation to other treatments, while the macroalgae waste dietary inclusion generated larvae with low fat content (14.7 ± 1.5 %_DM on average). Interestingly, it was observed that the addition of aquaculture wastes, even in small inclusion levels (between 15 % and 25 % on wet basis), reduced the concentration of saturated fatty acids in the larvae (especially lauric acid and pentadecanoic acid). It was concluded that BSF larvae are able to bioconvert varied aquaculture waste streams and it is possible to produce tailored larval biomass by adding such waste streams in their diets, enabling the production of a protein ingredient with specific traits to be used in aquafeeds.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"43 ","pages":"Article 102961"},"PeriodicalIF":3.2,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144570833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Using invasive weed (Bidens pilosa L.) as a feed supplement to promote Nile tilapia (Oreochromis niloticus) health","authors":"Nantaporn Sutthi ,&nbsp;Eakapol Wangkahart ,&nbsp;Wipavee Thaimuangphol ,&nbsp;Prasoborn Rinthong ,&nbsp;Laorsri Sanoamuang","doi":"10.1016/j.aqrep.2025.102967","DOIUrl":"10.1016/j.aqrep.2025.102967","url":null,"abstract":"<div><div><em>Bidens pilosa</em> is a widespread invasive species typically regarded as a weed with no economic value. Repurposing this plant as a functional feed ingredient presents a sustainable, cost-effective approach for aquaculture. This study evaluated the effects of adding dietary <em>B. pilosa</em> leaf ethanol extract on growth performance, immune responses, gene expression, and disease resistance in Nile tilapia. Experimental diets supplemented a basal feed with <em>B. pilosa</em> extract at 2.5 g/kg (T2), 5.0 g/kg (T3), and 10.0 g/kg (T4); the control group (T1) received no supplementation. After a 60-day feeding trial, fish in the T2 exhibited significantly higher weight gain, daily growth rate, and an improved feed conversion ratio (FCR) compared with the control group (<em>P</em> &lt; 0.05). Additionally, the T2 group had the highest fillet yield and the lowest carcass yield (<em>P</em> &lt; 0.05). Biochemical analyses revealed that the highest extract level (T4) induced oxidative stress and immune dysregulation, including both immune suppression and potential ineffective immunity, evidenced by elevated aspartate aminotransferase (AST) and malondialdehyde (MDA) levels, alongside decreased lysozyme activity (LZM), myeloperoxidase (MPO), red blood cell (RBC) count, and hemoglobin (Hb) concentration (<em>P</em> &lt; 0.05). In contrast, the T2 group exhibited the highest post-challenge survival (53.33 ± 15.27 %) against <em>Streptococcus agalactiae</em> and upregulated growth hormone (GH) and glutathione peroxidase (GPx) gene expression (<em>P</em> &lt; 0.05). Quadratic regression analysis estimated the optimal inclusion level at 4.29–4.58 g/kg. These findings demonstrate that supplementation at 2.5 g/kg enhances growth, immune competence, and disease resistance in Nile tilapia, whereas excessive inclusion (10 g/kg) may provoke oxidative stress, dual immune suppression, and inflammatory toxicity, underscoring the importance of precise dosage optimization for sustainable aquaculture.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"43 ","pages":"Article 102967"},"PeriodicalIF":3.2,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144557115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of dietary Alpinia oxyphylla extract on growth indices, muscle composition, antioxidant status, immunity, and ammonia resistance of juvenile ivory shell (Babylonia areolata)","authors":"Teng Li ,&nbsp;Yao-Peng Lu ,&nbsp;Zi-Hang Yu ,&nbsp;Hui Guo ,&nbsp;Ze-Long Zhang ,&nbsp;Pei-Hua Zheng ,&nbsp;Jun-Tao Li ,&nbsp;Li-Xuan Wang ,&nbsp;Xiao-Wen Zhu ,&nbsp;Jian-An Xian","doi":"10.1016/j.aqrep.2025.102965","DOIUrl":"10.1016/j.aqrep.2025.102965","url":null,"abstract":"<div><div>This study evaluated the impacts of dietary <em>Alpinia oxyphylla</em> extract (AO) on growth, muscle composition, antioxidant status, immunity, and ammonia resistance of juvenile ivory shell (<em>Babylonia areolata</em>). Six experimental diets (AO1, AO2, AO3, AO4, AO5, and AO6) with varying AO levels (0, 1, 2, 4, 6, and 10 g/kg diet) were fed to the ivory shell for 8 weeks. The weight gain rate and specific growth rate were higher in AO3 and AO4 groups. Muscle crude protein content was significantly higher in AO4 and AO5 groups, while crude lipid content significantly decreased in AO4 group compared to the control group. Superoxide dismutase (SOD) activity was increased in AO-fed <em>B. areolata</em>, with the highest values in the AO3 group. Catalase (CAT) activity was significantly increased in AO3, AO4, AO5, and AO6 groups. Total antioxidant capacity (T-AOC) was significantly higher and malondialdehyde (MDA) content was significantly lower, in AO3 and AO4 groups compared to the control group. The highest acid phosphatase (ACP) value was recorded in AO3, AO4, and AO5 groups, while alkaline phosphatase (AKP) activity was significantly higher in AO4, AO5, and AO6 groups compared to the control group. AO significantly induced the expression of <em>SOD</em>, <em>GST</em>, <em>CYP450</em>, <em>ACP</em>, and <em>CYC</em> in hepatopancreas. After ammonia stress, <em>B. areolata</em> fed with 2–4 g/kg AO showed a higher survival rate compared to the control group. These results suggest that dietary AO supplementation at 2.26 g/kg AO improves growth, muscle composition, immunity, and ammonia resistance in <em>B. areolata</em>.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"43 ","pages":"Article 102965"},"PeriodicalIF":3.2,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144557117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Astaxanthin from Phaffia rhodozyma screened by metagenome sequencing improves the growth performance, intestinal health, and immunity of juvenile Opsariichthys bidens","authors":"Muyan Li ,&nbsp;Weishao Bu ,&nbsp;Zifeng Yin ,&nbsp;Muhammad Jawad ,&nbsp;Lingzhan Xue ,&nbsp;Gaoxiong Zeng ,&nbsp;Meiying Wu ,&nbsp;Zhang Lu ,&nbsp;Junqiang Qiu ,&nbsp;Mingyou Li","doi":"10.1016/j.aqrep.2025.102960","DOIUrl":"10.1016/j.aqrep.2025.102960","url":null,"abstract":"<div><div>Intestinal flora, known as the \"second genome,\" play an important role in growth, development, barrier protection, and immunological tolerance in aquatic organisms. Intestinal flora of cultured fish can be influenced by various factors such as culture environment, growth stage, growth rate, and gender. In this study, we used a metagenome sequence to analyze the intestinal flora of male and female Chinese hook snout carp (<em>Opsariichthys bidens</em>) across different growth stages. This study aims to identify the genera that serve as sources of dietary nutrient supplementation to improve fish health status and growth performance. The results showed no significant differences in the overall intestinal flora of male and female <em>O. bidens</em>. However, variations in the growth stage may cause changes in the dominant phyla and functional dissimilarities between the two genders. Among the identified genera, <em>Phaffia rhodozyma</em> was screened as a genus considered to have the potential for nutrient supplementation. In order to validate its efficacy, varying concentrations of <em>P. rhodozyma</em> were supplemented into the diet to assess the culture performance of <em>O. bidens</em>. Our results demonstrated that the 0.5 % dietary supplementation of <em>P. rhodozyma</em> significantly improved growth performance indicators (WGR, SCR, and FCR) of <em>O. bidens</em> as compared to the control group (<em>p</em> &lt; 0.05). Moreover, it’s also enhanced the reproductive performance in females as well as increased the antioxidant capacity and immune resistance. Based on growth performance, 0.5 % was determined to be the optimal supplementation level of <em>P. rhodozyma</em>.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"43 ","pages":"Article 102960"},"PeriodicalIF":3.2,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144557217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sodium butyrate mitigates aflatoxin B1 toxicity in largemouth bass","authors":"Dongqiang Hou ,&nbsp;Hongxia Zhao ,&nbsp;Kai Peng ,&nbsp;Bing Chen ,&nbsp;Junru Hu ,&nbsp;Xifeng Zhu ,&nbsp;Guoxia Wang ,&nbsp;Junming Cao ,&nbsp;Wen Huang","doi":"10.1016/j.aqrep.2025.102966","DOIUrl":"10.1016/j.aqrep.2025.102966","url":null,"abstract":"<div><div>The aim of this study was to investigate the effects of aflatoxin B1 (AFB1) on growth performance, lipid metabolism, oxidative stress, histopathology, and gut microbiota composition in largemouth bass, and to evaluate the mitigation effect of sodium butyrate (SB). The initial body weight of largemouth bass was 3.38 ± 0.10 g, and the experimental period was 56 days. The experiments were divided into three groups: CON (basal diet without AFB1), AFB1 (basal diet +1.0 mg/kg AFB1), and SB (basal diet +1.0 mg/kg AFB1 +2.0 g/kg SB). The results showed that AFB1 significantly reduced the rate of weight gain in largemouth bass compared with CON, whereas there was no significant difference in the SB supplemented group. AFB1 decreased serum TG levels and increased CHO, HDL, and LDL levels, whereas SB addition did not mitigate these effects. In addition, blood glucose levels were also significantly lower in the AFB1 and SB groups compared to CON. In terms of oxidative stress, AFB1 exposure increased the activities of liver antioxidant enzymes, including GSH-Px, ACP, and AKP, while SB supplementation increased the activity of intestinal catalase CAT, both of which significantly increased the content of MDA. Histopathological results showed that AFB1 increased inflammatory responses in the gut and liver, which were attenuated by SB addition. In terms of gut microbial composition, AFB1 decreased the relative abundance of gut microbes in largemouth bass, whereas SB addition decreased the relative abundance of Firmicutes and Mycoplasma genera and increased the relative abundance of Proteobacteria. In conclusion, SB alleviated the negative effects of AFB1 on largemouth bass to some extent, especially in promoting growth performance, gut microbiota, and reducing inflammatory responses, providing a scientific basis for mitigating the adverse effects of AFB1 in the aquaculture industry.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"43 ","pages":"Article 102966"},"PeriodicalIF":3.2,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144557183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}