Aquaculture Reports最新文献

{"title":"Aquaculture performance of artificial interspecific backcross groups between the hybrid of Crassostrea hongkongensis × C. ariakensis and the two parental species","authors":"Yanping Qin ,&nbsp;Qinggan Xing ,&nbsp;Xingyou Li ,&nbsp;Jiangwei Li ,&nbsp;Weitao Wan ,&nbsp;Chao Yue ,&nbsp;Haitao Ma ,&nbsp;Jun Li ,&nbsp;Ying Pan ,&nbsp;Ziniu Yu ,&nbsp;Yuehuan Zhang","doi":"10.1016/j.aqrep.2025.102926","DOIUrl":"10.1016/j.aqrep.2025.102926","url":null,"abstract":"<div><div><em>Crassostrea hongkongensis</em> is popular in southern China for its white flesh color, unique flavor, and delicious taste. However, the disadvantages of slow growth, poor tolerance, and high mortality limit its production and market sales. The application of genetic breeding techniques to breed new breeding lines with fast growth and low mortality is an important approach to solve these industrial challenges, including interspecific backcross breeding. In this study, we established eight experimental groups (HH-<em>C. hongkonggensis</em> ♀ × <em>C. hongkonggensis</em> ♂; HA-<em>C. hongkonggensis</em> ♀ × <em>C. ariakensis</em>♂; AA-<em>C. ariakensis</em> ♂ × <em>C. ariakensis</em>; A/HA-<em>C. ariakensis</em>♀ × HA ♂; HA/HA-HA ♀ × HA ♂; H/HA-<em>C. hongkonggensis</em> ♀ × HA ♂; HA/H- HA ♀ × <em>C. hongkonggensis</em>♂ and HA/A-HA ♀ × <em>C. ariakensis</em> ♂), tracked and compared their phenotypic traits. The results confirmed that the hatching parameters of the interspecific backcross groups (HA/H, HA/A, H/HA and A/HA) were significantly lower than those of the pure parental progeny groups (HH and AA). Although HA/A did not always show a significant growth advantage in the larval stage, it exhibited the largest shell height during the marine culture period (except on the 210th day), and showed a significant shell height advantage on the 360th day (<em>P</em> &lt; 0.05). Moreover, there were obvious backcross advantages during the marine culture period, with backcross advantages (BAs) in shell height of 7.30 %, 17.66 %, 16.44 % and 10.95 % from the 90th to the 360th days, respectively. In addition, HA/A consistently ranked in the top three in terms of the incremental survival rate, being significantly higher than the grandparental offsprings (HH and AA), with HA/A showing the highest survival rates on the 210th and 360th days. Similarly, backcross advantages were observed at all time points, with BAs of 3.42 %, 5.96 %, 6.29 % and 0.43 %, respectively. Gonad development statistics and paraffin section results showed that all backcross groups were fertile and produced many normal gametes, with little difference in the ratios of males to females among the eight groups. In summary, the HA/A group showed significant backcross advantages in both growth and survival, with the best overall performance. This makes it a promising new strain that could help address the industrial problems of <em>C. hongkongensis</em> and has the potential for large-scale application.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"43 ","pages":"Article 102926"},"PeriodicalIF":3.2,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144297845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Culture performance and financial business of leopard coral (Plectropomus leopardus), humpback (Cromileptes altivelis), and brown-marbled (Epinephelus fuscoguttatus) grouper nursery","authors":"Belinda Astari ,&nbsp;Darmawan Setia Budi ,&nbsp;Suko Ismi ,&nbsp;Irzal Effendi ,&nbsp;Tatag Budiardi ,&nbsp;Iis Diatin ,&nbsp;Apriana Vinasyiam ,&nbsp;Ketut Sugama","doi":"10.1016/j.aqrep.2025.102923","DOIUrl":"10.1016/j.aqrep.2025.102923","url":null,"abstract":"<div><div>The nursery phase is critical in grouper aquaculture, influencing survival rates, growth performance, and economic feasibility. This study evaluates the culture performance and financial viability of three economically valuable grouper species: Leopard coral grouper (<em>Plectropomus leopardus</em>), humpback grouper (<em>Cromileptes altivelis</em>), and brown-marbled grouper (<em>Epinephelus fuscoguttatus</em>) over a 90-day nursery period. Results showed that brown-marbled grouper exhibited the highest survival rate (82.5 %) and growth performance, achieving the most significant final length (12.32 cm) and body weight (14.02 g). However, it also had the highest feed conversion ratio (FCR = 2.81), indicating lower feed efficiency. Leopard coral grouper demonstrated a balanced growth rate with the lowest FCR (2.00), making it the most cost-effective feed utilization. Financial analysis revealed that leopard coral grouper had the highest profitability, with the most significant net present value (NPV = US$ 65,067) and internal rate of return (IRR = 91.70 %), along with the shortest payback period (1.45 years). Sensitivity analysis indicated that feed costs and market price fluctuations significantly influenced financial viability. The research's findings show that leopard coral grouper is the most profitable species for nursery production, having the highest income, profit, net present value, and IRR. However, brown-marbled grouper has the lowest production cost per unit, making it a feasible option for budget-conscious farmers. While brown-marbled grouper grew the fastest in terms of length and weight, leopard coral grouper maintained competitive growth performance while also having the highest market value, making it the most economically advantageous option. To improve grouper farming's financial resilience, future initiatives should maximize feed efficiency, minimize mortality rates, and employ sustainable aquaculture practices.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"43 ","pages":"Article 102923"},"PeriodicalIF":3.2,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144297949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of different carbohydrate levels on glycolipid metabolism and liver health of channel catfish (Ictalurus punctatus)","authors":"Xiaochen Ma ,&nbsp;Qisheng Lu ,&nbsp;Guoli Han ,&nbsp;Jinglu Jia ,&nbsp;Jingyue Cao ,&nbsp;Haokun Liu ,&nbsp;Junyan Jin ,&nbsp;Zhimin Zhang ,&nbsp;Yunxia Yang ,&nbsp;Xiaoming Zhu ,&nbsp;Dong Han ,&nbsp;Shouqi Xie","doi":"10.1016/j.aqrep.2025.102921","DOIUrl":"10.1016/j.aqrep.2025.102921","url":null,"abstract":"<div><div>This study evaluated the effects of dietary carbohydrate levels on glycolipid metabolism and liver health of channel catfish. Six isonitrogenous and isolipidic diets with corn starch contents of 10 % (C10), 15 % (C15), 20 % (C20), 25 % (C25), 30 % (C30) and 35 % (C35) were set up. After 8-week feeding trial, there was no significant difference in the growth performance of all groups (<em>P</em> &gt; 0.05). The study showed that C30 and C35 groups significantly increased plasma glucose levels, activated the expression of glycolysis-related genes (<em>gk</em>, <em>hk</em>, <em>pklr</em>, <em>pfk</em>) and mitochondrial oxidative phosphorylation related genes (<em>ndufa1</em>, <em>sdhb</em>, <em>sdha</em>, <em>cytb-c1</em>, <em>cox</em>, <em>atp5fa1</em>, <em>atp5f1b</em>), and repressed the expression of gluconeogenesis-related genes (<em>pc</em>, <em>fbp</em>, <em>pepck</em>, <em>g6</em>p) in the channel catfish (<em>P</em> &lt; 0.05). The upregulation of glycogen synthesis-related genes (<em>ppp1r3g</em>, <em>ppp1r3c</em>, <em>ppp1r3b</em>, <em>gys, gnl, ugp2a, ugp2b</em>) contributed to a significant increase in hepatic glycogen content. In addition, C30 and C35 groups significantly increased the expression of pentose phosphate pathway related genes (<em>g6pd</em>, <em>6gpd</em>), while C10 to C25 groups glucose metabolism-related pathways did not exhibit significant differences. Hepatic triglyceride and cholesterol results showed that C30 and C35 groups induced excessive lipogenesis, activated the expression of lipogenesis-related genes (<em>acc</em>, <em>acly</em>, <em>vldlr</em>) and cholesterol synthesis-related genes (<em>srebf2</em>, <em>hmgcr</em>, <em>sqle</em>). In C30 and C35 groups also increased the hepatic ALT, AST and MDA levels, activated the expression of injury-related genes (<em>a-sma</em>, <em>tgf-β</em>), inflammation-related genes (<em>tnf-α</em>, <em>il-1β</em>, <em>il-6</em>) and apoptosis-related genes (<em>baxb</em>, <em>caspase-3</em>, <em>caspase −10</em>) (<em>P</em> &lt; 0.05). C35 group significantly further increased the expression levels of stress genes such as <em>hamp</em>, <em>hsp70</em> and <em>hsp90</em> (<em>P</em> &lt; 0.05), while there were no significant differences in the levels of lactate and cortisol in all groups (<em>P</em> &gt; 0.05). In conclusion, our results suggest that dietary carbohydrate levels less than 30 % will not impair the health and growth performance of the channel catfish.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"43 ","pages":"Article 102921"},"PeriodicalIF":3.2,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144291473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of commercial feeds and natural diets on the culture performance and gut microbiota of Scylla paramamosain","authors":"Xia Zheng ,&nbsp;Dongping Zhou ,&nbsp;Sixiang Wang ,&nbsp;Xiaojing Dong ,&nbsp;Chaoyue Wang ,&nbsp;Zehao Li ,&nbsp;Lei Liu ,&nbsp;Changkao Mu ,&nbsp;Chunlin Wang","doi":"10.1016/j.aqrep.2025.102922","DOIUrl":"10.1016/j.aqrep.2025.102922","url":null,"abstract":"<div><div>In this study, we systematically investigated how different feed combinations affect the growth performance and gut microbiota composition of the mud crab <em>Scylla paramamosain</em> in both indoor cement tanks and outdoor earthen ponds. We employed high-throughput 16S rRNA sequencing to profile the bacterial communities, placing particular emphasis on correlations between feed nutritional components and key microbial taxa. Firmicutes, Proteobacteria, Bacteroidetes, and Cyanobacteria emerged as the predominant phyla. Crabs fed locally sourced natural feeds from Dongying showed relatively minor shifts in community structure, whereas those receiving artificial formulated feed exhibited significant divergences, as evidenced by principal coordinate analysis (PCoA) and non-metric multidimensional scaling (NMDS). In indoor cement tank trials, no significant differences were found in Chao1 among groups. However, the combination of <em>Mactra chinensis</em> + <em>Synechogobius hasta</em> + <em>Trachypenaeus curvirostris</em> group significantly elevated Pielou_e and Shannon diversity indices (<em>P</em> &lt; 0.05), while the Simpson index was higher in both this group and the <em>S. hasta</em> + <em>T. curvirostris</em> + corn + soybean meal combination (<em>P</em> &lt; 0.05). Similar trends were observed in outdoor earthen pond trials, where the <em>M. chinensis</em> + <em>S. hasta</em> + <em>T. curvirostris</em> group and the <em>S. hasta</em> + <em>T. curvirostris</em> + corn + soybean meal group exhibited significantly higher diversity indices (<em>P</em> &lt; 0.05). Moreover, several indicator genera (e.g., OM190, <em>Terrisporobacter</em>, <em>Paraclostridium</em>) were positively correlated with specific nutritional components, implying that balanced feed formulas can promote beneficial bacteria, potentially supporting molting and growth. Collectively, the <em>M. chinensis</em> + <em>S. hasta</em> + <em>T. curvirostris</em> feed combination yielded the most favorable results, highlighting its potential for enhancing both crab growth and gut microbiota diversity. These findings advance our understanding of how feed composition shapes the gut microbiota in <em>S. paramamosain</em> and offer a theoretical basis for optimizing feed formulations, thereby contributing to sustainable aquaculture in Dongying and similar regions.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"43 ","pages":"Article 102922"},"PeriodicalIF":3.2,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144279951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of carbon budgets and greenhouse gas fluxes in monoculture ponds with milkfish (Chanos chanos) and Taiwanese hard clams (Meretrix taiwanica)","authors":"Zhao-Jun Yong ,&nbsp;Wei-Jen Lin ,&nbsp;Shu-Chiu Hsieh ,&nbsp;Hsing-Juh Lin","doi":"10.1016/j.aqrep.2025.102924","DOIUrl":"10.1016/j.aqrep.2025.102924","url":null,"abstract":"<div><div>Aquaculture plays a vital role in meeting the growing demand for aquatic food. However, research on carbon fluxes in aquaculture ponds remains limited. This study constructed carbon budgets and determined greenhouse gas (GHG) emissions from monoculture ponds of Taiwanese hard clams (<em>Meretrix taiwanica</em>) and milkfish (<em>Chanos chanos</em>). Phytoplankton production constituted the major carbon input pathways in the clam (72–85 %) and fish (41–48 %) ponds. However, respiration offset most of the carbon, which turned the fish ponds into heterotrophic systems that emitted an average of 0.21 g C m⁻² d⁻¹. In contrast, the clam ponds remained autotrophic systems, functioning as a carbon sink with an average uptake of 0.06 g C m⁻² d⁻¹, even when accounting for the CO₂ release by biocalcification. The accumulated sediment significantly contributed to the carbon output pathways in the ponds, accounting for 62–77 % of those in the clam ponds and 23–52 % of those in the fish ponds. An unaccounted pathway of carbon input likely contributed to sediment accumulation in the clam ponds caused by the filter-feeding behavior of clams, which induces resuspension or the production of pseudofeces. On the other hand, feed input mainly contributed to sediment accumulation in the fish pond. Methane flux, biocalcification, water exchange, and rainwater accounted for relatively small portions of both carbon budgets. Based on the 100-year global warming potential (GWP₁₀₀), the GHG emissions during the culture period were 0.46 ± 0.75 g CO₂e m⁻² d⁻¹ in the clam ponds and 1.15 ± 1.15 g CO₂e m⁻² d⁻¹ in the fish ponds. Methane fluxes contribute relatively little to the overall GHG emissions from both ponds, while nitrous oxide fluxes can alter the fish ponds into a greater GHG source.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"43 ","pages":"Article 102924"},"PeriodicalIF":3.2,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144270402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of candidate pheromones and neurotransmitter regulation of agonistic behavior in Macrobrachium rosenbergii","authors":"Hangyu Qi ,&nbsp;Qi Shen ,&nbsp;Jiongying Yu ,&nbsp;Miaoying Cai ,&nbsp;Jianyu Song ,&nbsp;Junjie Wu ,&nbsp;Guoliang Yang ,&nbsp;Shaokui Yi","doi":"10.1016/j.aqrep.2025.102920","DOIUrl":"10.1016/j.aqrep.2025.102920","url":null,"abstract":"<div><div><em>Macrobrachium rosenbergii</em>, a crucial commercial prawn species in China, exhibits agonistic behavior under high-density culture conditions, which negatively impacts their integrity, survival, and growth, causing economic losses. This study utilized liquid chromatography mass spectrometry (LC-MS) to identify and classify the metabolic differences in urine and water mixtures of <em>M. rosenbergii</em> displaying agonistic behavior versus those without. Seventeen compounds, such as 4-Acetamidobutyric acid, 1-oleoyl-2-myristoyl-sn-glycero-3-phosphocholine, N-Acety-L-valine, 11-Dehydrocorticosterone, Sphinganine (Sph t18:0), and Acitretin, were identified as candidate pheromones associated with agonistic behavior, 4-Acetamidobutyric acid and Sph (t18:0) were hypothesized to be particularly significant. Additionally, the roles of serotonin (5-HT) and dopamine (DA) in agonistic behavior among <em>M. rosenbergii</em> males were evaluated. We found that 5-HT levels in hemolymph significantly decreased after fighting (<em>P</em> &lt; 0.05), while DA levels significantly increased (<em>P</em> &lt; 0.05) compared to the control group. The receptor genes of 5-HT and DA (5-HTRs and DARs) were further analyzed. Gene expression analyses of <em>M. rosenbergii</em> before and after agonistic behavior revealed that <em>Mr5-HTR1</em> exhibited significant changes in hemolymph and abdominal ganglia, while <em>Mr5-HTR2</em> varied across five tissues, and <em>MrDAR1</em> and <em>MrDAR2</em> showed significant changes in the brain and abdominal ganglia. To verify the impact of 5-HTRs on the agonistic behavior, 5-HTRs agonist and antagonist were in vivo injected. The results showed that the injection of 5-HTRs agonists significantly inhibited the occurrence of agonistic behavior in <em>M. rosenbergii</em>. This study deepens our understanding of the regulatory mechanisms of crustacean agonistic behavior and provides a theoretical basis for improving the crustacean farming management.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"43 ","pages":"Article 102920"},"PeriodicalIF":3.2,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144241132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of dietary carbohydrate level on growth performance, glucose metabolism, antioxidant capacity, immune response, and digestive function in coho salmon Oncorhynchus kisutch (Walbaum, 1792) fry","authors":"Hairui Yu ,&nbsp;Lingyao Li ,&nbsp;Leyong Yu ,&nbsp;Abdur Rahman ,&nbsp;Govindharajan Sattanathan","doi":"10.1016/j.aqrep.2025.102917","DOIUrl":"10.1016/j.aqrep.2025.102917","url":null,"abstract":"<div><div>A 10-week feeding trial was conducted to assess the effects of graded dietary carbohydrate levels on growth performance and antioxidant capacity in coho salmon (<em>Oncorhynchus kisutch</em>) fry. Six isonitrogenous (48.75 % crude protein) and isolipidic (12.98 % crude lipid) diets containing 0.00 %, 4.50 %, 9.00 %, 13.50 %, 18.00 %, and 22.50 % carbohydrate were formulated. A total of 900 fish (0.27 ± 0.01 g) were randomly distributed into 18 experimental tanks (240 L water volume), with triplicate groups per diet. Results showed that the final body weight (FBW), specific growth rate (SGR) and protein efficiency ratio (PER) increased significantly with dietary carbohydrate up to 13.5 % (<em>p</em> &lt; 0.05), while feed conversion ratio (FCR) decreased (<em>p</em> &lt; 0.05). No significant differences (<em>p</em> &gt; 0.05) were observed in intestosomatic index (ISI), hepatosomatic index (HSI) and condition factor (CF). Whole-body moisture and ash content remained unchanged, whereas crude lipid and crude protein content were significantly higher in groups fed 13.5 %, 18.0 % and 22.5 % carbohydrate compared to 0.0 % and 4.5 % group (<em>p</em> &lt; 0.05). Hepatic superoxide dismutase (SOD) and catalase (CAT), along with hexokinase(HK), pyruvate kinase (PK), fructose-1,6-biphosphatase (FBPase) activity, and glycogen content increased significantly up to 13.5 % carbohydrate (<em>p</em> &lt; 0.05). Hepatic malondialdehyde (MDA) content was lowest at 13.5 % carbohydrate levels. The activities of hepatic acid phosphatase (ACP), alkaline phosphatase (AKP) and lysozyme (LZM)(<em>p</em> &lt; 0.05) increased with carbohydrate levels (<em>p</em> &lt; 0.05). Intestinal amylase, lipase and protease activities peaked at 13.5 % carbohydrate levels (<em>p</em> &lt; 0.05). Broken-line regression analysis of SGR indicated an optimal dietary carbohydrate requirement of 14.15 % for coho salmon fry. This study provides concrete targets for optimizing dietary carbohydrate levels in coho salmon feed, while offering valuable insights for developing sustainable feeding strategies.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"43 ","pages":"Article 102917"},"PeriodicalIF":3.2,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144241306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of three genes in the methyl farnesoate signaling pathway from the Pacific whiteleg shrimp Litopenaeus vannamei and their roles in methyl farnesoate-mediated ovarian development","authors":"Jiahui Liu ,&nbsp;Zhiguo Hu ,&nbsp;Xusheng Guo ,&nbsp;Liangjie Zhao ,&nbsp;Chaoqun Su ,&nbsp;Wei Wang","doi":"10.1016/j.aqrep.2025.102914","DOIUrl":"10.1016/j.aqrep.2025.102914","url":null,"abstract":"<div><div>Methyl farnesoate (MF) is an essential regulator of development and reproduction in arthropods. This study identified the MF signaling response genes and analyzed their potential roles in ovarian development of the Pacific whiteleg shrimp <em>Litopenaeus vannamei</em>. Three critical genes, including Methoprene-tolerant (<em>LvMet</em>), Retinoid X receptor (<em>LvRXR</em>) and Krüppel homolog 1 (<em>LvKr-h1</em>), were identified from <em>L. vannamei</em>. Their expression increased and reached the peak at the ovarian mature stage. In vitro experiments revealed that <em>LvMet</em>, <em>LvRXR</em>, <em>LvKr-h1</em>, and vitellogenin genes (<em>LvVg1</em> and <em>LvVg2</em>) in the hepatopancreas explants were significantly induced by MF, while <em>LvMet</em> and <em>LvKr-h1</em> mRNA were significantly induced in the ovarian explants. In vivo injection of MF significantly stimulated <em>LvMet</em> expression in the ovaries, and induced the expression of <em>LvRXR</em> and <em>LvKr-h1</em> in the hepatopancreas and ovaries. When either <em>LvMet</em> or <em>LvRXR</em> was silenced, <em>LvKr-h1</em> expression was significantly decreased in the hepatopancreas. Silencing <em>LvKr-h1</em> expression also significantly decreased <em>LvMet</em> and <em>LvRXR</em> expression. Yeast two-hybrid experiment further proved that LvMet weakly interacted with LvRXR. In conclusion, <em>LvMet</em>, <em>LvRXR</em> and <em>LvKr-h1</em> constitute crucial components of the MF signaling pathway regulating ovarian development of <em>L. vannamei</em>, and LvMet forms a heterodimer with LvRXR in response to MF to activate the transcription of <em>LvKr-h1</em>.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"43 ","pages":"Article 102914"},"PeriodicalIF":3.2,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144241305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined prebiotic and multivitamin supplementation enhances growth, survival, and disease resistance of Asian seabass in floating cages","authors":"Mohammad Asadollahi ,&nbsp;Javad Baserh ,&nbsp;Faranak Abnaroodhelleh ,&nbsp;Masoud Baghaei Kordyani ,&nbsp;Mahsa Naderi Samani ,&nbsp;Maryam Dadar","doi":"10.1016/j.aqrep.2025.102919","DOIUrl":"10.1016/j.aqrep.2025.102919","url":null,"abstract":"<div><div>Sustainable alternatives to antibiotics are becoming more crucial in aquaculture for enhancing fish health and performance. This study aimed to evaluate the effects of dietary supplementation with antibiotics and a combination of prebiotics and multivitamins on the growth performance, survival, and disease resistance of Asian seabass (<em>Lates calcarifer</em>) cultured in polyethylene floating cages in the Persian Gulf, Qeshm Island, Iran. Over a 32-week trial, 100,000 seabass fingerlings were assigned to one of three dietary treatments: a control diet, a prebiotic/multivitamin-supplemented diet, or an antibiotic-supplemented diet. Key growth parameters, feed conversion ratio (FCR), and pathogens such as Vibrio spp. and Streptococcus spp. in dead fish were assessed through microbiological examination. The combined prebiotic/multivitamin group demonstrated significantly higher final weight (707.2 ± 30.77 g), survival rate (90.13 ± 0.76 %), specific growth rate (1.10 ± 0.01 %/day), and relative growth rate (1308.12 ± 2.69 %) compared to the control and antibiotic groups (P &lt; 0.05). This group also exhibited the lowest FCR (1.08 ± 0.01), indicating superior feed efficiency. In contrast to the other treatments, the prebiotic/multivitamin group showed no signs of <em>Streptococcus agalactiae</em> infection. Our study is among the few that apply these findings to the more diverse and practical setting of open-water cage aquaculture. These findings suggest that the combined prebiotic/multivitamin supplementation is an effective and sustainable alternative to antibiotics in cage-cultured Asian seabass for enhancing growth, feed efficiency, and resistance to <em>S. agalactiae</em> infection.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"43 ","pages":"Article 102919"},"PeriodicalIF":3.2,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144230749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of fish meal replacement by Clostridium autoethanogenum protein on growth performance, serum biochemistry, antioxidant capacity, immune responses and muscle quality in black carp (Mylopharyngodon piceus)","authors":"Yifan Fu ,&nbsp;Jiao Wei ,&nbsp;Hongrong Lin ,&nbsp;Jinjing Zhang ,&nbsp;Yuanyuan Zhang ,&nbsp;Jiaxing Yu ,&nbsp;Jie Li ,&nbsp;Mingxu Xie ,&nbsp;Xianping Shao ,&nbsp;Jinyun Ye ,&nbsp;Haifeng Mi ,&nbsp;Chenglong Wu ,&nbsp;Lu Zhang","doi":"10.1016/j.aqrep.2025.102892","DOIUrl":"10.1016/j.aqrep.2025.102892","url":null,"abstract":"<div><div>The study investigated the growth performance, serum biochemistry, antioxidant and immune responses, and muscle quality of black carp (<em>Mylopharyngodon piceus</em>) fed dietary fish meal replaced by <em>Clostridium autoethanogenum</em> protein (CAP) (0 %, 20 %, 40 %, 60 %, 80 %, and 100 %), which were named CAP0 (control), CAP20, CAP40, CAP60, CAP80, and CAP100, respectively. A total of 450 juvenile black carp (initial body weight: 5.34 ± 0.02 g) were randomly allocated into six experimental groups. Results showed there were no differences on growth in black carp fed six CAP-level diets. Contents of insulin-like growth factor Ⅰ (IGF-Ⅰ) and 5-hydroxytryptamine (5-HT) were increased in CAP60 group. CAP40 showed reduced activities of serum alanine aminotransferase (ALT), and aspartate aminotransferase (AST), while increase lysozyme (LZM), and immunoglobulin M (IgM) levels compared to CAP100 group. Although <em>kelch-like ECH associated protein 1α</em> (<em>keap1α</em>) were decreased in CAP60 group, g<em>lutathione peroxidase 1</em> (<em>gpx1</em>) mRNA levels were heightened in the hepatopancreas of CAP40 and CAP60 groups. There were no differences on activities of total superoxide dismutase (T-SOD) and catalase (CAT) among CAP0, CAP20 and CAP40 groups. CAP40 exhibited increased intestinal villi height (VH) values together with <em>claudin-5</em> (<em>cldn-5</em>) levels compared to CAP0 group. Meanwhile, CAP40 increased values of hardness and springiness, and expression levels of <em>collagen 1α1</em> (<em>col1α1</em>), <em>prolyl hydroxylase</em> (<em>phd</em>) and <em>lysyl oxidase</em> (<em>lox</em>) in muscle compared to CAP100 group. In conclusion, 40 % fish meal replacement with CAP could improve serum biochemical indices, antioxidant capability, immune responses, muscle texture and quality in black carp.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"43 ","pages":"Article 102892"},"PeriodicalIF":3.2,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144230723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}