Aquaculture Reports最新文献

{"title":"Effects of dietary soya-saponins on growth performance and intestinal health in juvenile largemouth bass (Micropterus salmoides)","authors":"Xinpeng Wang ,&nbsp;Jun Wen ,&nbsp;Ximing Hou ,&nbsp;Haiqing Wu ,&nbsp;Qianyu Zhou ,&nbsp;Xue Fu ,&nbsp;Chuyi Cui ,&nbsp;Shimei Lin ,&nbsp;Yongjun Chen ,&nbsp;Li Luo ,&nbsp;Qinghui Ai ,&nbsp;Yuanfa He","doi":"10.1016/j.aqrep.2025.103142","DOIUrl":"10.1016/j.aqrep.2025.103142","url":null,"abstract":"<div><div>This study investigated the effects of dietary soya-saponins (SS) on the growth and intestinal health of juvenile largemouth bass (<em>Micropterus salmoides</em>). Four isonitrogenous and isolipidic diets were formulated: a fish meal (FM)-based control diet and three experimental diets supplemented with 0.1 %, 0.2 %, and 0.6 % SS (SS0.1, SS0.2, and SS0.6, respectively). Largemouth bass with initial individual body weight of 7.60 ± 0.0 g were randomly allocated to four groups in triplicate with 20 individuals in each tank. The experiment lasted for 56 days. The results demonstrated that SS significantly reduced the growth performance compared with FM group (<em>P</em> &lt; 0.05). SS significantly reduced plica height and width, and goblet cell counts compared with FM group (<em>P</em> &lt; 0.05). Additionally, SS caused pathological changes in intestinal cells and organelles, including microvillar disorganization, nuclear pyknosis, mitochondrial cristolysis, and endoplasmic reticulum dilation, most severe in SS0.6 group. SS0.6 group markedly decreased serum lipopolysaccharide, D-lactate, and diamine oxidase compared with FM group (<em>P</em> &lt; 0.05). Furthermore, SS0.2 and SS0.6 groups significantly reduced serum acid and alkaline phosphatase activities and elevated alanine and aspartate aminotransferase and total bile acid levels compared with FM group (<em>P</em> &lt; 0.05). SS0.2 and SS0.6 groups significantly reduced intestinal superoxide dismutase, glutathione peroxidase, catalase, total antioxidant capacity compared with FM group (<em>P</em> &lt; 0.05). SS0.6 group significantly increased the abundance of potential pathogens (<em>Acinetobacter</em>, <em>Pseudomonas</em>, <em>Escherichia-Shigella</em>, and <em>Serratia</em>) compared with the FM group (<em>P</em> &lt; 0.05). SS0.6 and FM comparison group significantly downregulated the PI3K-Akt and B cell receptor signaling pathways. SS0.6 group markedly downregulated intestinal barrier function genes (<em>zo-1</em>, <em>claudin3</em>, <em>claudin8</em>, and <em>nectin4</em>), <em>il22</em> and <em>c3</em>, as well as significantly upregulated <em>cox2</em> and <em>ccl4</em> (<em>P</em> &lt; 0.05). In conclusion, dietary SS adversely affects the growth performance and intestinal health of juvenile largemouth bass, potentially via microbiota dysregulation and inflammatory responses.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"45 ","pages":"Article 103142"},"PeriodicalIF":3.7,"publicationDate":"2025-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145217077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-term intake of a high-carbohydrate diet induced steatosis, hepatitis, and fibrosis in the liver of juvenile Chinese soft-shelled turtle (Pelodiscus sinensis)","authors":"Jiarou Li ,&nbsp;Tianyu Liu ,&nbsp;Lei Li ,&nbsp;Tao Li ,&nbsp;Yujing Guan ,&nbsp;Mengjiao Song ,&nbsp;Yiwei Huang ,&nbsp;Haiyan Liu ,&nbsp;Ling Li ,&nbsp;Peiyu Zhang","doi":"10.1016/j.aqrep.2025.103146","DOIUrl":"10.1016/j.aqrep.2025.103146","url":null,"abstract":"<div><div>A high intake of carbohydrate has been associated with liver health problems in aquaculture animals. The objective of this study was to evaluate whether prolonged consumption of a high-carbohydrate diet (HCD) would induce the metabolic disorders and histopathological abnormalities in the liver that characterize nonalcoholic steatohepatitis (NASH) in <em>P. sinensis</em> juveniles. Two isonitrogenous and isolipidic diets containing 14 % (normal carbohydrate diet, CON) and 28 % corn starch (HCD) were formulated to feed juvenile <em>P. sinensis</em> (5.15 ± 0.05 g) for 60 d. The experimental turtles were sampled on the 20th d, 40th d, and 60th d. The results indicated that significant hepatic lipid accumulation (triglyceride content and Oil red O-stained area percentage) was observed in the HCD group on the 40th d and 60th d compared to the CON group (<em>P</em> &lt; 0.05), which were probably associated with significantly higher mRNA levels of genes involved in fatty acids uptake and lipid synthesis in liver (<em>P</em> &lt; 0.05), while it was not related with lipolysis in liver. The more interesting is that the hepatic TG content in the HCD group were not further increased from 40th d to 60th d, which was partly attributed to the enhanced triglyceride (TG) excretion from liver into plasma on the 60th d as reflected by significantly increased levels of plasma low-density lipoprotein (LDL), plasma TG content, and apolipoprotein B (<em>apob</em>) transcript on this day (<em>P</em> &lt; 0.05). Moreover, a 40-d and 60-d intake of HCD induced hepatic inflammation as indicated by significantly higher mRNA levels of pro-inflammatory cytokines and M1 macrophage number (fluorescence intensity). Furthermore, HCD caused significantly higher protein expressions of hepatic fibrosis markers (α-smooth muscle actin and vimentin), accompanied with higher fibrosis index (Masson-stained collagen) and higher mRNA levels of fibrosis-related genes (<em>P</em> &lt; 0.05). Taken together, these results demonstrated that long-term intake of HCD in juvenile <em>P. sinensis</em> induced hepatic steatosis and inflammation, which are key contributors to the development of fibrosis.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"45 ","pages":"Article 103146"},"PeriodicalIF":3.7,"publicationDate":"2025-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145217107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative evaluation of growth performance, flesh quality, and gut microbiota among three strains of largemouth bass (Micropterus salmoides)","authors":"Shiliang Jia ,&nbsp;Qi Gao ,&nbsp;Xiaoming Chen ,&nbsp;Gaohua Yao ,&nbsp;Yating Zhao ,&nbsp;Qinghui Meng ,&nbsp;Xingwei Xiang ,&nbsp;Xueyan Ding ,&nbsp;Hong Zeng ,&nbsp;Fan Zhou","doi":"10.1016/j.aqrep.2025.103134","DOIUrl":"10.1016/j.aqrep.2025.103134","url":null,"abstract":"<div><div>A comparative analysis was conducted to compare the growth performance, flesh quality, and gut microbiota of three strains of largemouth bass (<em>Micropterus salmoides</em>), Zhelu No.1 (ZL), the Reservoir group (RG), and Youlu No.3 (YL). For this purpose, three strains of largemouth bass raised under identical conditions until they reached 150 days of age. The dorsal muscle of fish was collected and analyzed for the proximate composition, texture properties, free amino acids, volatile organic compounds and ATP-related compounds. Meanwhile, the gut microbiota of different strains was also detected. The results demonstrated that the ZL strain exhibited higher body weight, fat content and condition factor compared to the RG and YL strains. Furthermore, the levels of umami amino acids, total amino acids (TAA), and essential amino acids (EAA) in the ZL strain were significantly higher than those in RG and YL strains. Moreover, HxR and Hx contents in the ZL strain were significantly lower than in the YL strain. GC‑IMS analysis further revealed that ethanol and benzaldehyde levels were higher in the ZL than in the other two strains. Additionally, gut microbiota analysis revealed that the ZL strain was characterized by a community dominated by <em>Stenotrophomonas</em> (60.1 %) and <em>Chryseobacterium</em> (23.6 %), a composition that may contribute to its superior growth performance. In summary, the ZL strain displayed integrated advantages in growth efficiency, flavor profile, and nutritional quality, underscoring its potential as a preferred alternative in largemouth bass aquaculture.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"45 ","pages":"Article 103134"},"PeriodicalIF":3.7,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145217060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of compound probiotics on the growth of Conger myriaster, water quality parameters, and microbial communities in industrial recirculating aquaculture systems","authors":"Xinyu Zhao ,&nbsp;Bao Shi ,&nbsp;Chenbai Wang ,&nbsp;Hanliang Cheng ,&nbsp;Chenggang Wang ,&nbsp;Xiaodong Ma ,&nbsp;Kewen Yan ,&nbsp;Yan Chen","doi":"10.1016/j.aqrep.2025.103138","DOIUrl":"10.1016/j.aqrep.2025.103138","url":null,"abstract":"<div><div>The aim of this study was to determine the effects of adding compound probiotics to culture water on fish growth, water quality parameters, and microbial communities in industrial recirculating aquaculture systems (RAS) of <em>Conger myriaster</em>. Two systems were established: a control group system without any compound probiotics and a test group system with compound probiotics added to the culture water (1.0 × 10⁹ CFU/mL of viable bacteria, a dosage of 8 mL/m³, and a frequency of 1 time/7 d). The addition of compound probiotics to culture water in industrial RAS significantly improved the growth performance of <em>C. myriaster</em> (<em>P</em> &lt; 0.05). Additionally, it significantly reduced the levels of ammonia nitrogen (NH₄⁺-N), nitrate nitrogen (NO₃⁻-N), nitrite nitrogen (NO₂⁻-N), total nitrogen (TN), and chemical oxygen demand (COD) in the culture tanks and biofilters in the test group on day 70 (<em>P</em> &lt; 0.05). It also increased the average removal rates of NH₄⁺-N, NO₃⁻-N, NO₂⁻-N, TN^, COD, total phosphorus (TP), and PO₄^3--P in the culture tanks and biofilter water in the test group. The relative abundance of beneficial bacteria related to water purification was significantly increased in the culture water, biofilter water, and biofilter carriers in the test group at the end of the experiment (<em>P</em> &lt; 0.05). Furthermore, the compound probiotics significantly reduced the influence of environmental factors on the microbial community in the water of the test group. Therefore, it played a dual role in regulating water quality and bacterial community structure. The bacterial communities in the water of the culture tanks and biofilters of the test group were mainly involved in functional roles such as chemoheterotrophy, aerobic chemoheterotrophy, and nitrite respiration. Overall, adding compound probiotics to culture water significantly improved the <em>C. myriaster</em> growth and water quality parameters, regulated the structure and function of the microbial community in the RAS, and promoted the degradation of nitrogenous compounds and organic matter.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"45 ","pages":"Article 103138"},"PeriodicalIF":3.7,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145217071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The use of meiofauna as an indicator of fish-farms impact: a case study from the middle eastern Adriatic Sea","authors":"Bruna Petani ,&nbsp;Ivan Župan ,&nbsp;Simone Mirto ,&nbsp;Elena Manini ,&nbsp;Anamaria Baričević ,&nbsp;Bosiljka Mustać ,&nbsp;Francesca Ape","doi":"10.1016/j.aqrep.2025.103133","DOIUrl":"10.1016/j.aqrep.2025.103133","url":null,"abstract":"<div><div>This investigation aimed to analyze for the first time the impact of fish farming on the biochemical composition of sedimentary organic matter and meiofaunal assemblages on a sea bass/sea bream farm located in the central Croatian waters. We compared two aquaculture sites: one near the cages (ST 1) and one directly beneath them (ST 2), along with a control site (Control) located 700 m away. Our findings revealed significant organic matter enhancement in the sediments surrounding the cages, leading to increased total meiofaunal abundance but decreased diversity. Furthermore, meiofaunal community structure differed among aquaculture and control sediments, particularly in terms of rare taxa (less than 1 % abundance). Increased Nematoda abundances contrasted with the decline or absence of more sensitive taxa like Copepoda and Kinorhyncha near the fish farm. These results highlight the importance of considering meiofaunal rare taxa, such as Kinorhyncha, for better understanding benthic responses to increased anthropogenic impacts and support the suggestion of other authors to use the nematodes to kinorhyncs ratio as a useful indicator of organic enrichment associated with aquaculture activities.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"45 ","pages":"Article 103133"},"PeriodicalIF":3.7,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145217108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Field surveillance has revealed a high prevalence of PRV-3 in clinically healthy salmonids in Italy","authors":"Petra Drzewnioková ,&nbsp;Romy Lucon Xiccato ,&nbsp;Francesca Bruno ,&nbsp;Claudia Casarotto ,&nbsp;Matteo Mazzucato ,&nbsp;Eleonora Franzago ,&nbsp;Marica Toson ,&nbsp;Niccolò Vendramin ,&nbsp;Anna Toffan","doi":"10.1016/j.aqrep.2025.103131","DOIUrl":"10.1016/j.aqrep.2025.103131","url":null,"abstract":"<div><div>Piscine orthoreovirus genotype 3 (PRV-3) has been reported in several European countries, and in Italy it was detected only once in 2017 without any associated disease outbreak. This study aimed to investigate the presence of PRV-3 in the area with the highest salmonids production in Italy. Samples were collected between September 2023 and May 2024 from three regions of northeastern Italy. Pooled samples were screened by a generic PRV real-time RT-PCR, and positive samples were subsequently characterized as PRV-3. The geographic distribution of PRV-3 was visualised and risk factors analysis was performed. Of the 267 tested specimens, 37.1 % yielded positive results, corresponding to 43.6 % positive farms in the investigated area. Phylogenetic analyses confirmed that the Italian PRV-3 strains clustered within group 3b. The highest positivity was observed in <em>Salmo trutta</em> (49.1 %), followed by <em>Oncorhynchus mykiss</em> (38.7 %) and <em>Salmo marmoratus</em> (24.3 %). No signs of diseases or history of mortality were observed from the positive batches of fish. Apparently healthy wild fish also tested positive. These results suggest that PRV-3 is widespread in both farmed and wild salmonid populations in the investigated area and that it does not cause specific health issues under the current Italian farming conditions and wild environment.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"45 ","pages":"Article 103131"},"PeriodicalIF":3.7,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145217109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elevated water nutrient levels promote co-occurrence of sediment microbiota in white-leg shrimp (Litopenaeus vannamei) culture ponds","authors":"Liang Wu ,&nbsp;Xiaoli Shi ,&nbsp;Jian Wang ,&nbsp;Xiafei Zheng ,&nbsp;Xueqiao Qian ,&nbsp;Shouqi Xie ,&nbsp;Baojun Shi ,&nbsp;Ruiming Xiao ,&nbsp;Bo Wang ,&nbsp;Yingli Lian ,&nbsp;Jiayi Tang","doi":"10.1016/j.aqrep.2025.103121","DOIUrl":"10.1016/j.aqrep.2025.103121","url":null,"abstract":"<div><div>Aquatic microbiota plays crucial roles in biogeochemical processes. However, whether the water nutrients and co-occurrence of sediment microbiota in white-leg shrimp culture ponds increases during culture is unclear. We investigated the water nutrients and sediment microbiota in six white-leg shrimp culture ponds during culture using high-throughput 16S rRNA gene sequencing. Our results showed that water total nitrogen (TN), NO₃-N, NO₂-N, and total phosphorus were typically increased during the white-leg shrimp culture process. Although the overall structure of the sediment microbiota in the ponds was not altered during culture, the relative abundance of <em>Caldicoprobacter</em>, <em>Dechloromonas</em>, <em>Erysipelothrix</em>, <em>Hydrogenophaga</em>, and <em>Mariniradius</em> gradually decreased. Particularly, the co-occurrence of sediment microbiota among the different ponds was enhanced during the culture process. The concentrations of TN, NO₃-N, and total organic carbon in the water were significantly positively correlated with the average weighted degree, average clustering coefficient, and total triangles of the co-occurrence networks of the sediment microbiota. These results imply the accumulation of nutrients in the pond water probably promoted the co-occurrence of sediment microbiota, which implying the convergence of the sediment microbiota across ponds. Therefore, microbial cooccurrence has potential to be an index to measure the nutritional status of aquaculture ponds.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"45 ","pages":"Article 103121"},"PeriodicalIF":3.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145217073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the nutritional value of processed lupin meal (Lupinus albus) with exogenous enzymes, as feed ingredient in European sea bass (Dicentrarchus labrax) aquafeeds","authors":"Antigoni Vasilaki ,&nbsp;Ioannis Nengas ,&nbsp;Dimitra Kogiannou ,&nbsp;Morgane Henry ,&nbsp;Chrysanthi Nikoloudaki ,&nbsp;Petros Chronopoulos ,&nbsp;Panagiotis Berillis ,&nbsp;Eleni Golomazou ,&nbsp;Eleni Fountoulaki ,&nbsp;Ioannis T. Karapanagiotidis ,&nbsp;Elena Mente","doi":"10.1016/j.aqrep.2025.103135","DOIUrl":"10.1016/j.aqrep.2025.103135","url":null,"abstract":"<div><div>The aim of the current study was to evaluate the nutritional value of <em>Lupinus albus</em> processed with exogenous enzymes as replacer of soybean meal. Six hundred European sea bass (11.2 ± 0.0 g) were distributed in 12 tanks and fed the 4 experimental diets containing soybean meal to processed lupin meal at ratios: Control (15 %: 0 %), Lupin 1 (5 %: 7.5 %), Lupin 2 (2.5 %: 10 %), Lupin 3 (0 %: 12.5 %) in triplicate groups for 83 d. The results showed significantly higher final body weight (<em>P</em> = 0.050) and specific growth rate (<em>P</em> = 0.036) and lower feed conversion ratio (<em>P</em> = 0.047) for the group fed the Lupin 1 diet compared to the Control diet. Significantly higher apparent digestibility coefficient of crude protein (<em>P</em> = 0.043) was noted for the group tested with the Lupin 3 diet compared to Control and apparent digestibility coefficient of fat was significantly higher for groups fed the Lupin 1 (<em>P</em> = 0.015) and Lupin 3 (<em>P</em> = 0.000) diets compared to Control. Essential amino acid deposition was higher for the group fed the Lupin 2 diet related to the Control group and the same was observed for trypsin activity in the pyloric caeca (<em>P</em> = 0.031) and intestine (<em>P</em> = 0.016) of fish. Positive effect was observed in the immune status of fish fed the processed lupin meal and no histopathological alterations were determined. Comprehensively, the results proved the potential of processed lupin meal at 12.5 % to totally replace soybean meal in feed formulation for Mediterranean aquaculture marine species.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"45 ","pages":"Article 103135"},"PeriodicalIF":3.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145217074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacological characterization of the neuropeptide F receptor (NPFR) in Macrobrachium rosenbergii","authors":"Yue-Miao Yu ,&nbsp;Ke-Xin Yu ,&nbsp;Li Sun ,&nbsp;Dong-Ji Song ,&nbsp;Da-Yan Hu ,&nbsp;Shao-Kui Yi ,&nbsp;Yang Li","doi":"10.1016/j.aqrep.2025.103132","DOIUrl":"10.1016/j.aqrep.2025.103132","url":null,"abstract":"<div><div>The neuropeptide F receptor (NPFR) is a critical regulator of energy homeostasis in invertebrates, orchestrating feeding behavior, nutrient assimilation, and reproductive functions. We report the successful cloning and functional characterization of NPFR from the giant freshwater prawn <em>Macrobrachium rosenbergii</em>. Structural analysis confirmed that the cloned sequence is a typical GPCR with seven transmembrane domains and conserved motifs DRY and NPLLY. Sequence alignment showed high homology (&gt;90 %) with other long-arm shrimp species, supporting evolutionary conservation within the <em>Palaemonidae</em> family. Quantitative real-time PCR (qRT-PCR) revealed ubiquitous expression of mrNPFR, with the highest levels in the intestinal tract, followed by hemolymph and eyestalk ganglia, suggesting multifunctional roles in energy homeostasis and neuroendocrine regulation. Functional assays using luciferase reporter systems demonstrated that NPF-II was the most potent cAMP inhibitor, while NPF-III exhibited the highest efficacy in activating the MAPK/ERK pathway. NPF-I showed the highest activation efficacy for the NFAT pathway. This study provides foundational data for understanding NPFR’s physiological functions in <em>M. rosenbergii</em> and offers insights for precision feeding strategies in aquaculture.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"45 ","pages":"Article 103132"},"PeriodicalIF":3.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145217075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"On-site biofouling removal enhances the nutritional and gustatory quality of oysters from suspension aquaculture and the recovery of essential biomolecules for human consumption","authors":"Yuji Hatakeyama ,&nbsp;Chikako Maruo ,&nbsp;Megumu Fujibayashi ,&nbsp;Ramon Filgueira ,&nbsp;Takashi Sakamaki","doi":"10.1016/j.aqrep.2025.103129","DOIUrl":"10.1016/j.aqrep.2025.103129","url":null,"abstract":"<div><div>We experimentally tested the effects of on-site biofouling removal (BR) via heat treatment on the biomolecule contents of Pacific oysters and the bay-scale recovery of these biomolecules through the harvest of farmed oysters from suspension aquaculture in a temperate bay. At harvest after an 18-month cultivation period, oysters treated at the 13th month showed 18 % higher soft body weights and increased contents of certain biomolecules compared to untreated (UN) oysters. Specifically, BR oysters had 15 % higher eicosapentaenoic acid (EPA) content and 13 % higher docosahexaenoic acid (DHA) content than UN oysters. The glycogen content of BR oysters was 54 % higher, suggesting enhanced energy storage and potentially improved sweetness. BR likely reduced food competition between oysters and fouling organisms, predominantly mussels, leading to the higher biomolecule contents. If BR were expanded to cover the entire bay (from the current 10–100 %), the recovery of EPA and DHA through the harvest of farmed oysters would increase by 28 %, reaching 869 kg in a 1.5-year production cycle. Biofouling removal (BR) did not significantly affect the content of essential amino acids (AAs), gustatory-related AAs, or taurine in oysters. However, due to improved oyster biomass production, BR is estimated to potentially increase the recovery of these biomolecules by approximately 12 %. In conclusion, on-site BR is a straightforward and effective method for enhancing the levels of certain nutritional and gustatory-related biomolecules in cultivated oysters while simultaneously increasing the recovery of nutritionally important biomolecules for human consumption.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"45 ","pages":"Article 103129"},"PeriodicalIF":3.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145217076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}