Aquaculture Reports最新文献

{"title":"The effects of Triclosan (TCS) on the survival, serum biomarkers, antioxidant defense, and apoptosis of mud crab (Scylla paramamosain)","authors":"Yue Wang ,&nbsp;Ting-Ting Hou ,&nbsp;Xue-Feng Song ,&nbsp;Zhi-Xing Su ,&nbsp;Jia-Yuan Xu ,&nbsp;Xiao-Kang Lv ,&nbsp;Shuai Zhou ,&nbsp;Bian-Bian Zhang ,&nbsp;Yan-Ming Sui ,&nbsp;Li-Guo Yang","doi":"10.1016/j.aqrep.2025.102719","DOIUrl":"10.1016/j.aqrep.2025.102719","url":null,"abstract":"<div><div>Triclosan (TCS), a widely used antimicrobial agent, is known to have toxic effects on aquatic organisms, potentially disrupting reproduction, development, and behavior. However, its impact on marine invertebrates, particularly crustaceans, remains understudied. To evaluate the effects of TCS, juvenile mud crabs (<em>Scylla paramamosain</em>) were exposed to TCS concentrations ranging from 50 to 5000 μg/L over a 96-hour period in this study. Results showed that low TCS concentrations had no significant short-term effects on the crabs, whereas moderate to high concentrations caused disruptions in hemolymph parameters, increased oxidative stress, and reduced antioxidant enzyme activity, ultimately leading to mortality. Exposure to these higher concentrations also led to the upregulation of heat shock proteins (HSPs) and antioxidant enzyme mRNA expression as part of the stress response, alongside the increased expression of pro-apoptotic genes and suppression of anti-apoptotic genes. The effects were generally similar between sexes, although females showed greater susceptibility than males. Additionally, TCS exposure altered serum cholesterol levels in females but had no effect on males, suggesting a possible estrogenic effect of TCS that requires further investigation. This study provides important data for assessing the risks of TCS in aquatic environments and has significant implications for the conservation of estuarine ecosystems and fisheries resources.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"41 ","pages":"Article 102719"},"PeriodicalIF":3.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143518951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation between SNPs in promoter region of gene, L-Type lectin (LvLTLC1) and the resistance trait of shrimp, Litopenaeus vannamei, against vibrios","authors":"Shuyang Wen ,&nbsp;Lihong Yuan ,&nbsp;Jiayue Yin ,&nbsp;Chaoqun Hu ,&nbsp;Xin Zhang ,&nbsp;Yunyi Huang ,&nbsp;Chujie Zhao ,&nbsp;Liyan Liu ,&nbsp;Peng Luo","doi":"10.1016/j.aqrep.2025.102725","DOIUrl":"10.1016/j.aqrep.2025.102725","url":null,"abstract":"<div><div>Pacific white shrimp, <em>Litopenaeus vannamei</em> (<em>L. vannamei</em>), is the most economically valuable shrimp species in world. Bacterial diseases mainly caused by various vibrios bring huge economic loss in shrimp aquaculture. Previous research has confirmed that <em>LvLTLC1</em> gene coding a L-type lectin of <em>L. vannamei</em> plays a pivotal role in the resistance of shrimp against pathogenic vibrios, which implies the potential of <em>LvLTLC1</em> as a target gene to develop SNP markers for genetic breeding of the shrimp. In this study, pan-vibrio resistance (PVR) trait of <em>L. vannamei</em> was quantitatively evaluated by vibrios loads in hepatopancreas and shrimp individuals were divided into vibrios-resistance (VR) groups and vibrios-susceptible (VS) groups according to their vibrios-resistance performance (VRP). Expression assay of <em>LvLTLC1</em> showed that mRNA levels of <em>LvLTLC1</em> in the hepatopancreas of the VR group was significantly higher than that in the VS groups. Totally, 24 SNPs were identified in the promoter regions of <em>LvLTLC1</em> in shrimp individuals. Correlation analysis between the genotypes of these single nucleotide polymorphism (SNPs) and the VRP revealed that a homozygous GG genotype in SNP8 (g.–256 A/G) were significantly correlated with the VRP (<em>P</em> &lt; 0.05). Linkage disequilibrium (LD) analysis revealed a high linkage block of SNP9 (g.–249 T/G) and SNP10 (g.–235 A/G), and a TT/GG combined genotype of SNP9 and SNP10 was further discovered to be significantly associated with strong VRP (<em>P</em> &lt; 0.05). Furthermore, the variations in motifs caused by SNP8, SNP9, and SNP10 led to changes of binding patterns of transcription factors. The present study first revealed the presence of abundant SNPs in a gene promoter region of shrimp <em>L. vannamei</em> and found that specific genotypes of SNPs in <em>LvLTLC1</em> promoter confer strong VRP in the shrimp. The homozygous GG genotype in SNP8 and the TT/GG genotype combination of SNP9 and SNP10 can be served as two potential molecular markers for the PVR trait in genetic breeding of <em>L. vannamei.</em></div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"41 ","pages":"Article 102725"},"PeriodicalIF":3.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143520147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanisms of immune responses in juvenile Acanthopagrus latus to Aeromonas hydrophila infection revealed by multi-tissue transcriptomic analysis","authors":"Hong-Di Fan ,&nbsp;Min Liu ,&nbsp;Yuan-Ming Zhao ,&nbsp;Xu Wang ,&nbsp;Jia Lin ,&nbsp;Qing Zhu ,&nbsp;Wen Huang ,&nbsp;Jiang-Hai Wang","doi":"10.1016/j.aqrep.2025.102724","DOIUrl":"10.1016/j.aqrep.2025.102724","url":null,"abstract":"<div><div>Yellowfin seabream (<em>Acanthopagrus latus</em>) is an economically important species extensively cultivated along the southeast coastal sea of China. This species is frequently afflicted by infections from <em>Aeromonas hydrophila</em>, a pathogen that causes widespread morbidity and mortality. In this study, an infection model was established by administering an intraperitoneal injection of <em>A. hydrophila</em> into yellowfin seabream. Subsequent observations and histopathological assessments revealed considerable damages of multi-tissues after the infection. Biochemical serum assays and measurements of immune-related enzyme activities indicated evident metabolic disturbances and inflammatory pathways in the affected fish. RNA sequencing analysis identified 1615 differentially expressed genes (DEGs) in the liver and 2578 DEGs in the intestine. KEGG mapping revealed significantly regulated pathways, including the C-type lectin receptor signaling pathway and MAPK signaling pathway in the liver, and calcium signaling pathway and cytokine-cytokine receptor interaction in the intestine. A protein-protein interaction network was also constructed for pivotal genes, such as <em>cxcr4b</em> and <em>cd44b</em>, which may play crucial roles in the immune and inflammatory pathways of yellowfin seabream. The transcriptomic results were further corroborated by quantitative real-time PCR tests of some specific genes. These novel data have revealed the immune-related pathways and key regulatory genes activated by <em>A. hydrophila</em> infection and may offer a valuable insight into the immune response mechanisms of yellowfin seabream.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"41 ","pages":"Article 102724"},"PeriodicalIF":3.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143527378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary ursodeoxycholic acid supplementation enhanced nonspecific immunity, antioxidant capacity, and anti-inflammatory-related gene expression in juvenile large yellow croaker (Larimichthys crocea)","authors":"Aijun Zhu ,&nbsp;Weiye Li ,&nbsp;Xiaolin Zhang ,&nbsp;Xiaojun Yan","doi":"10.1016/j.aqrep.2025.102698","DOIUrl":"10.1016/j.aqrep.2025.102698","url":null,"abstract":"<div><div>Bile acids (BAs) are known to confer health benefits across various fish species; nevertheless, the beneficial effects of ursodeoxycholic acid (UDCA) on immunity are largely unknown. This research investigated the impact of UDCA supplementation on nonspecific immunity, antioxidant capacity, and immune-related gene expression in juvenile large yellow croaker (<em>Larimichthys crocea</em>). The experimental diets were formulated with different UDCA supplement levels: 0 mg/kg (CON), 50 mg/kg (UL), 100 mg/kg (UM), and 500 mg/kg (UH). A total of four hundred fish were randomly distributed into 16 tanks with 25 fish per tank. Each diet was fed to four replicate groups twice daily. After a 70-day feeding trial, the results indicated that the UM diet significantly increased the percentage of nitroblue tetrazolium (NBT)-positive cells, serum lysozyme (LZM) activity, alternative complement pathway (ACP) activity, liver antioxidant capacity, and survival rate postcatch stress (<em>P</em> &lt; 0.05). To elucidate the mechanisms underlying the immunomodulatory effects of UDCA, we conducted RNA sequencing of liver, intestinal, olfactory, and brain tissues from the CON and UM groups. Results indicated that UDCA upregulated genes associated with nonspecific immune responses, particularly those related to complement pathways (<em>c3</em>, <em>c4a</em>, and <em>cfh</em>), antioxidants (<em>sod1</em>, <em>sod2</em>, and <em>gpx7</em>), and lysozyme production (<em>lyg</em>). Conversely, the expression of chemokine genes (<em>mrc1</em>, <em>ccl21</em>, and <em>ccr9</em>) in the intestine significantly decreased (<em>P</em> &lt; 0.05). Weighted gene coexpression network analysis (WGCNA) identified gene clusters linked to immunity primarily through the NF-κB pathway (<em>chuk</em>, <em>nfkb1</em>, <em>traf2</em>, and <em>ikbkb</em>). <em>In vitro</em> experiments with primary hepatocytes demonstrated that incubation with 10 μM UDCA markedly downregulated cytokine production genes (<em>il1b</em> and <em>nfkb1</em>) but upregulated antioxidant genes (<em>gpx7</em>, <em>cat</em>, <em>sod1</em>, and <em>nrf2</em>) following 1 mg/mL LPS challenge. In summary, the present study indicated that UDCA supplementation positively influences nonspecific immunity, enhances antioxidant responses, and reduces proinflammatory signaling in juvenile large yellow croaker.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"41 ","pages":"Article 102698"},"PeriodicalIF":3.2,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143512294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of dietary yellow mealworm Tenebrio molitor meal and selenium on the growth performance, digestive and absorptive enzyme activity, immune response, skin color, and muscle quality of large yellow croaker Larimichthys crocea","authors":"Peng Qu ,&nbsp;Zhiyu Zhang ,&nbsp;Yang Wu ,&nbsp;Shuangjie Tian ,&nbsp;Jing Yuan ,&nbsp;Zhenhua Wu ,&nbsp;Peng Chen ,&nbsp;Dong Huang ,&nbsp;Mingzhu Pan ,&nbsp;Kangsen Mai ,&nbsp;Wenbing Zhang","doi":"10.1016/j.aqrep.2025.102696","DOIUrl":"10.1016/j.aqrep.2025.102696","url":null,"abstract":"<div><div>The present study aimed to investigate the effects of increasing levels of yellow mealworm <em>Tenebrio molitor</em> (TM) meal as a replacement of dietary fishmeal (FM) and different levels of dietary selenium supplementation on the growth performance, digestive and absorptive enzyme activity, immune response, skin color and muscle quality of large yellow croaker <em>Larimichthys crocea</em> (initial body weight: 189.97 ± 1.01 g). Using a 3 × 2 factorial design, six isonitrogenous (about 47 % of crude protein) and isolipidic (about 10 % of crude lipid) diets were formulated with 3 replacement levels of FM by TM (15 %, 30 % and 45 %) and two supplemented levels of selenium (0.67 mg/kg and 3.78 mg/kg) in the form of sodium selenite. After an 80-day feeding trial, the results showed that 30 % of replacement of dietary FM by TM did not significantly influence the growth performance and feed utilization of large yellow croaker. The supplement level of 1.7 mg/kg Se significantly improved the weight gain and ventral and caudal skin yellow value (<em>P</em> &lt; 0.05). This selenium level led to a decrease in serum triglyceride, malondialdehyde, and 8-hydroxydeoxyguanosine, while increased the activities of intestinal chymotrypsin, γ-glutamyltransferase, acid phosphatase and lysozyme (<em>P</em> &lt; 0.05). The replacement of FM with TM at 15 % and 30 % levels significantly increased the expression of <em>nuclear factor erythroid 2-related factor 2</em> (<em>P</em> &lt; 0.05). The 1.7 mg/kg dietary selenium reduced the expression of <em>nuclear factor-κb</em> and <em>kelch-like ech-associated protein 1</em> in both intestine and liver. In conclusion, up to 30 % replacement of dietary FM by TM showed no significant adverse effects on growth and feed utilization of large yellow croaker. Supplementation of 1.7 mg/kg dietary selenium significantly enhanced the growth, feed utilization, anti-oxidative ability, and muscle quality of large yellow croaker. However, there were no interactions between dietary FM replacement level and Se supplement level regarding growth performance, immune response, and muscle quality of large yellow croaker. The Se could mitigate the negative growth effects of over-high dietary TM on large yellow croaker.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"41 ","pages":"Article 102696"},"PeriodicalIF":3.2,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143512293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary geniposide supplementation alleviates hepatic lipid deposition and oxidative stress induced by high-fat diet in juvenile tiger puffer, Takifugu rubripes","authors":"Haoran Sun ,&nbsp;Yonghui Wang ,&nbsp;Yinghui Liu ,&nbsp;Yi Liu ,&nbsp;Junjie Zhang","doi":"10.1016/j.aqrep.2025.102711","DOIUrl":"10.1016/j.aqrep.2025.102711","url":null,"abstract":"<div><div>High-fat diet has a negative impact on fish health, making it crucial to implement measures to mitigate these effects. This study aimed to assess the effects of geniposide administration in a high-fat diet (HFD) on the growth performance, serum biochemical parameters, hepatic histological characteristics, and lipid metabolism of juvenile tiger puffer (<em>Takifugu rubripes</em>). A total of 270 fish (initial body weight: 3.69 ± 0.07 g) were randomly divided into three groups with triplicate tanks. They were hand-fed to apparent satiety for 8 weeks with control diet (10 % crude lipid, CON), high-fat diet (15 % crude lipid, HFD), and HFD with 400-mg/kg geniposide (HFDG), respectively. The results showed that no significant differences in growth performance were observed among the three groups (<em>P</em> &gt; 0.05). However, the crude lipid contents of whole body and liver, serum levels of triglycerides (TG), alanine transaminase (ALT) and aspartate transaminase (AST), hepatic malonaldehyde (MDA) content and total superoxide dismutase (T-SOD) were significantly higher in HFD group than that in CON group (<em>P</em> &lt; 0.05). Compared with HFD group, the HFDG group had notably lower hepatic crude lipid content, serum and hepatic TG and total cholesterol levels, serum AST levels, and hepatic T-SOD activity (<em>P</em> &lt; 0.05). H&amp;E staining revealed hepatocyte enlargement and cytoplasmic vacuolization in the HFD group, while HFD supplemented with geniposide ameliorated these conditions. Transcriptomic sequencing of fish liver tissues revealed 350 differentially expressed genes between the HFD and HFDG groups. KEGG analysis showed that these genes were enriched in lipid metabolism-related pathways, including glycerolipid metabolism, fatty acid degradation, and steroid hormone biosynthesis. Compared with HFD group, the HFDG group showed significant downregulation of genes involved in lipogenesis (<em>dgat1</em>, <em>acc</em>, <em>fasn</em>, <em>chrebp</em>, <em>lxrα,</em> and <em>fabp-1</em>) and upregulation of genes involved in lipolysis (<em>agtl</em>, <em>lpl</em>, and <em>pparα</em>) (<em>P</em> &lt; 0.05). In conclusion, 400 mg/kg geniposide supplementation in HFD alleviated the HFD-induced hepatic lipid deposition and oxidative stress in <em>T. rubripes</em> by inhibiting lipogenesis and promoting lipolysis.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"41 ","pages":"Article 102711"},"PeriodicalIF":3.2,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143512291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interaction between dietary L-carnitine and lipid levels on growth, lipid metabolism, and antioxidant capacity in juvenile golden pompano (Trachinotus ovatus)","authors":"Yuanming Yi ,&nbsp;Haoyong Ma ,&nbsp;Beiping Tan ,&nbsp;Hongjie Wei ,&nbsp;Jiahua Wu ,&nbsp;Minling Mao ,&nbsp;Jianwei Wu ,&nbsp;Shuyan Chi ,&nbsp;Hongyu Liu ,&nbsp;Qihui Yang","doi":"10.1016/j.aqrep.2025.102714","DOIUrl":"10.1016/j.aqrep.2025.102714","url":null,"abstract":"<div><div>To evaluate the interaction between dietary L-carnitine (LC) and lipid levels on the lipid metabolism, antioxidant capacity and growth of juvenile golden pompano (<em>Trachinotus ovatus</em>), an 8-week trial was conducted. Six experimental diets were formulated by adding 0.02 % and 0.04 % LC to feeds with lipid levels of 9 %, 12 %, and 15 %, resulting in the following groups: C2L9, C4L9, C2L12, C4L12, C2L15, and C4L15. The results showed the weight gain rate and specific growth rate were significantly higher in the C4L15 group (<em>P</em> &lt; 0.05), whereas the feed conversion ratio was significantly lower in the C2L12 group. Serum total cholesterol, alanine aminotransferase, and aspartate aminotransferase activities were higher in the L12 and L15 groups compared to the L9 group. The expression of <em>cpt1</em> and <em>atgl</em> mRNA in the C4L12 group was significantly upregulated, whereas the expression of <em>acc</em> mRNA was significantly downregulated. The levels of lipid and LC significantly influenced malondialdehyde content and superoxide dismutase activity in the liver. At the same LC level, amylase and lipase activities tended to increase with higher lipid levels. Likewise, intestinal protease activity increased with increasing LC levels at the same lipid level. At the same LC level, foregut fold height significantly increased with higher lipid levels. Additionally, intestinal wall thickness was greatest in the C2L12 group. Higher lipid levels decreased gut flora diversity and abundance, promoting the growth of the pathogenic bacterium <em>Photobacterium</em>. Conversely, elevated LC levels positively impacted the abundance of the beneficial bacterium <em>Mycoplasma</em> and effectively reduced the abundance of the pathogenic bacterium <em>Vibrio</em>. Therefore, adding carnitine to high-lipid diets can help reduce lipid peroxidation in the liver of <em>T. ovatus</em>, improve lipid and carbohydrate utilization, promote intestinal development, and enhance the growth of beneficial gut bacteria.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"41 ","pages":"Article 102714"},"PeriodicalIF":3.2,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143509298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-throughput based detection of eukaryotic pathogens for sea cucumber in Dalian costal area","authors":"Xiaotong Zhu ,&nbsp;Jinjin Wang ,&nbsp;Xiaojun Rong ,&nbsp;Meijie Liao ,&nbsp;Jianlong Ge ,&nbsp;Yingeng Wang ,&nbsp;Lisha Guan ,&nbsp;Bin Li ,&nbsp;Qi Liu ,&nbsp;Xintao Zhao","doi":"10.1016/j.aqrep.2025.102715","DOIUrl":"10.1016/j.aqrep.2025.102715","url":null,"abstract":"<div><div>Sea cucumbers inhabit benthic environments and primarily feed on seabed sediments. Analyzing the community composition of eukaryotic species in their culture environment provides a scientific basis for assessing culture-related risks. In this study, we employed high-throughput sequencing (HTS) techniques to investigate potential pathogens associated with fungal and parasitic diseases affecting sea cucumbers in the Dalian coastal area. Concurrently, various environmental factors were measured seasonally to evaluate their potential impacts on the relative abundance of the identified pathogens. The results indicated that two fungal phyla, Ascomycota and Basidiomycota, associated with sea cucumber body vesicular syndrome (BVS), were present in considerable abundance in both the water and sediment of the national farm. Additionally, Acoela, known parasitic turbellarians of sea cucumbers, were also detected in significant numbers in the sediment, particularly during the summer months. The results of principal coordinate analysis (PCoA) revealed that the structure of eukaryotic communities in both water and sediment exhibited significant seasonal variation. Furthermore, temperature and total organic carbon (TOC) were found to have significant positive effects on the seasonal relative abundance of Acoela in the sediment. Conversely, pH appeared to negatively influence the relative abundance of the identified fungal phyla, while the eutrophication index (E) exhibited a positive effect. These findings demonstrate the feasibility of utilizing HTS techniques for detecting pathogenic eukaryotes in sea cucumber farms. We recommend incorporating high-throughput-based pathogenic monitoring into management strategies at sea cucumber farms to mitigate losses resulting from infectious disease outbreaks. For future research, it is essential to develop more efficient primers or primer mixes for detecting additional pathogenic threats and to enhance disease control measures in sea cucumber farms, particularly those focused on broodstock production.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"41 ","pages":"Article 102715"},"PeriodicalIF":3.2,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143512292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Krill supplement does not reduce virus load or heart pathology during PRV-3 infection in rainbow trout under experimental conditions","authors":"Juliane Sørensen ,&nbsp;Tine Moesgaard Iburg ,&nbsp;Giulia Zarantonello ,&nbsp;Torunn Forberg ,&nbsp;Argelia Cuenca ,&nbsp;Niccoló Vendramin","doi":"10.1016/j.aqrep.2025.102681","DOIUrl":"10.1016/j.aqrep.2025.102681","url":null,"abstract":"<div><div>Piscine orthoreoviruses pose a significant challenge to salmonid aquaculture, as there are currently no preventive measures, like vaccines, available. <em>Piscine orthoreovirus</em> genotype 3 (PRV-3), now renamed <em>Orthoreovirus piscis</em>, has been linked to disease in rainbow trout (<em>Oncorhynchus mykiss</em>) farmed in recirculating aquaculture systems (RAS), and has a high prevalence in Danish RAS. Previous studies have demonstrated that functional feeds can reduce the heart inflammation caused by PRV-1 in Atlantic salmon (<em>Salmo salar</em>). This study aims to determine whether functional feeds have a similar effect on rainbow trout infected with PRV-3. Specifically, we investigated the impact of a krill meal enriched diet on heart inflammation, anemia, and viral RNA load in rainbow trout exposed to PRV-3. The fish were pre-fed with either a control diet or enriched diet for six weeks prior to challenge with PRV-3. The results showed no significant differences in viral RNA load, hematocrit levels or heart pathology between the diets. However, PRV-3 exposed fish fed with the enriched diet exhibited significantly higher weight gain compared to those on the control diet. These findings suggest that while krill meal enhances growth performance, it does not reduce PRV-3-induced heart pathology in rainbow trout. Further research is needed to explore the underlying mechanisms and potential benefits of dietary interventions in managing PRV-3 infections.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"41 ","pages":"Article 102681"},"PeriodicalIF":3.2,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143474326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recuperative effects of herbal feed supplement on Hypohthalmichthys molitrix: Improvement in physiology and histopathology against waterborne-induced heavy metals toxicity","authors":"Sawera Maqaddas ,&nbsp;Syed Makhdoom Hussain ,&nbsp;Shafaqat Ali ,&nbsp;Ameer Fawad Zahoor ,&nbsp;Ebru Yilmaz ,&nbsp;Pallab K. Sarker ,&nbsp;Mohammad Abul Farah ,&nbsp;Eman Naeem","doi":"10.1016/j.aqrep.2025.102705","DOIUrl":"10.1016/j.aqrep.2025.102705","url":null,"abstract":"<div><div>Heavy metal (HM) contamination poses a substantial risk to both aquatic ecosystems and the environment. Therefore, this investigation explored the potential recuperative effects of dietary turmeric (TU) and ginger (GN) on silver carp (<em>Hypohthalmichthys molitrix</em>) fingerlings. These fingerlings were exposed to mixtures of HMs, specifically Pb (1.71 mg/L), Cd (2.47 mg/L), Cr (0.1 mg/L), and Zn (0.4 mg/L), and evaluated for growth, hematology, carcass composition, hepatic antioxidant indices, and histopathological profile. A total of 210 fish (7.16 ± 0.01 g/fish) were randomly divided into seven groups, each in triplicate. Fingerlings were allocated to negative control (without HM), positive control (with HM), and five treatment groups (T¹-T⁵). The treatment groups fed diets supplemented with 1 % TU, 1 % GN, 1 % TU+HM, 1 % GN+HM and combination of 1 % TU and GN + HM, respectively. Waterborne HM mixture negatively affected the growth performance, carcass, blood indices, antioxidant enzymes and histopathology. In contrast, fish groups fed with alone TU and GN supplementation resulted in increased growth, hematological indices, crude protein levels, and antioxidant enzyme activity of <em>H. molitrix</em>. In addition, GN, TU, and their combination supplementation with HM recuperated the above-mentioned parameters. Moreover, degeneration of hepatocytes, necrosis, and central vein damage were observed in HM-exposed fish and improved in the T3, T4, and T5 groups. The research findings demonstrated that feeding canola meal supplemented with 1 % GN, TU, or their combination to <em>H. molitrix</em> mitigated HM-induced negative effects on growth, carcass composition, antioxidant activity, hematology, and histopathological alterations in the liver. Conclusively, herbal supplements such as GN and TU exhibit promising benefits for promoting overall health and mitigating HM toxicity in silver carp.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"41 ","pages":"Article 102705"},"PeriodicalIF":3.2,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143474329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}