Glutamate and serotonin enhance feeding performance, digestive enzyme activity, and immunity in the female giant freshwater prawn, Macrobrachium rosenbergii

IF 3.7 2区农林科学 Q1 FISHERIES

Aquaculture Reports Pub Date : 2025-07-31 DOI:10.1016/j.aqrep.2025.103002

Thiraphon Deethaisong , Warinthip Vetkama , Pannasa Manjit , Ruchanok Tinikul , Prasert Sobhon , Yotsawan Tinikul

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引用次数: 0

Abstract

This study investigated the presence of glutamate (Glu) and serotonin (5-HT) in the digestive organs and their effects on feeding performance, digestive enzyme activity, and immunity throughout the ovarian cycle in female giant freshwater prawns (Macrobrachium rosenbergii). By high-performance liquid chromatography (HPLC), Glu and 5-HT were detected in the foregut, midgut, hindgut, and hepatopancreas, at the highest concentrations in the late ovarian stage and the lowest concentrations in the early ovarian stage. Through immunohistochemistry, the presence of Glu-immunoreactivity (-ir) and 5-HT-ir were observed in all digestive organs, and their intensities were shown to correlate with the levels detected by those observed via HPLC. Prawns injected with Glu and 5-HT at the doses of 2.5 × 10⁻⁶ and 2.5 × 10⁻⁷ mol/prawn, markedly increased food consumption, gastric emptying, and midgut index as well as enhanced amylase, protease, and lipase activities, compared with the control group (P < 0.05). Glu and 5-HT also enhanced epithelial cell height, villi height, and crypt depth and promoted the reactivity of PAS/AB in the digestive organs. In addition, treatment with Glu or 5-HT significantly upregulated M. rosenbergii NPF (MrNPF) and immune-related transcripts (P < 0.05). Collectively, these findings demonstrated the promising effects of Glu and 5-HT at a dose of 2.5 × 10⁻⁶ mol/prawn in stimulating feeding, digestive activities, and immunity, which could potentially be used to enhance feeding, growth, and maintain the health status of female prawn in aquaculture.

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谷氨酸和血清素可提高罗氏沼虾雌虾的摄食性能、消化酶活性和免疫力

本研究探讨了罗氏沼虾（Macrobrachium rosenbergii）雌性淡水巨虾消化器官中谷氨酸（Glu）和5-羟色胺（5-HT）的存在及其对卵巢周期内摄食性能、消化酶活性和免疫力的影响。高效液相色谱法（HPLC）检测前肠、中肠、后肠和肝胰脏中Glu和5-HT的浓度在卵巢晚期最高，在卵巢早期最低。通过免疫组织化学，在所有消化器官中均观察到glu -免疫反应性（-ir）和5-HT-ir的存在，其强度与HPLC检测到的水平相关。与对照组相比，注射2.5 × 10−6和2.5 × 10−7 mol/只Glu和5-HT显著增加了对虾的食量、胃排空和中肠指数，并增强了淀粉酶、蛋白酶和脂肪酶的活性（P <； 0.05）。Glu和5-HT还能提高上皮细胞高度、绒毛高度和隐窝深度，促进消化器官中PAS/AB的反应性。此外，Glu或5-HT治疗显著上调罗氏m.r NPF （MrNPF）和免疫相关转录物（P <； 0.05）。综上所示，在2.5 × 10−6 mol/对虾的剂量下，Glu和5-HT在刺激摄食、消化活性和免疫方面具有良好的效果，可能用于促进水产养殖中雌性对虾的摄食、生长和维持健康状态。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Aquaculture Reports Agricultural and Biological Sciences-Animal Science and Zoology

CiteScore

5.90

自引率

8.10%

发文量

469

审稿时长

77 days

期刊介绍： Aquaculture Reports will publish original research papers and reviews documenting outstanding science with a regional context and focus, answering the need for high quality information on novel species, systems and regions in emerging areas of aquaculture research and development, such as integrated multi-trophic aquaculture, urban aquaculture, ornamental, unfed aquaculture, offshore aquaculture and others. Papers having industry research as priority and encompassing product development research or current industry practice are encouraged.