Aquaculture Reports最新文献

{"title":"Composite single-cell protein as a sustainable fishmeal substitute: Enhancing growth and health in largemouth bass (Micropterus salmoides)","authors":"Jian Chen ,&nbsp;Jianqiang Huang ,&nbsp;Ling Pan ,&nbsp;Fuping Song ,&nbsp;Yonghua Hu ,&nbsp;Shuang Zhang ,&nbsp;Yan Wang","doi":"10.1016/j.aqrep.2025.102810","DOIUrl":"10.1016/j.aqrep.2025.102810","url":null,"abstract":"<div><div>This study evaluated the potential of a composite single-cell protein (CSCP) containing <em>Chlorella vulgaris</em> and <em>Clostridium autoethanogenum</em> at a 1:4 ratio as a substitute for fishmeal (FM) in largemouth bass (<em>Micropterus salmoides</em>) feed. A 56-day trial assessed CSCP's effects on disease resistance, antioxidant capacity, immune response, digestive function, and intestinal microbiota. Three diets were formulated: the control diet (FM52) with a high proportion of FM, the FM26 diet with 50 % of the FM replaced by CSCP, and the FM0 diet with complete FM replacement by CSCP. Three groups of 360 largemouth bass (initial average weight: 4.00 ± 0.03 g) were assigned to diets with three replicates of 40 fish each. Exposure to <em>Aeromonas hydrophila</em> resulted in higher survival rates (SR) in CSCP-supplemented groups, with the FM26 group showing the highest SR. Serum biochemical analysis indicated that CSCP improved lipid metabolism and liver health, evidenced by changes in triglycerides, cholesterol, alanine aminotransferase and aspartate aminotransferase. Antioxidant defense was strengthened with increased superoxide dismutase, peroxidase, and glutathione activities, and reduced malondialdehyde, indicating reduced oxidative stress. Gene expression analysis showed that the anti-apoptotic <em>bcl-2</em> and anti-inflammatory <em>il-10</em> genes were up-regulated, while the pro-apoptotic <em>casp3</em> and <em>casp9</em> as well as the pro-inflammatory <em>tnf-α</em> and <em>il-8</em> genes were down-regulated. Intestinal digestive enzyme activities (trypsin, α-amylase, lipase) were enhanced, and intestinal microbiota composition was altered, with an increase in potential beneficial bacteria (<em>Cetobacterium</em>, <em>Clostridium_sensu_stricto_1</em>) and a reduction in potential pathogens (<em>Mycoplasma</em>, <em>Aeromonas</em>) in the FM26 group. Functional predictions indicated enhanced pathways in “Cell motility” and “Immune system,” with enrichment of the NOD-like receptor signaling pathway. In conclusion, CSCP is a promising FM substitute, improving disease resistance, antioxidant capacity, immune response, digestive function, and intestinal microbiota in largemouth bass, with the FM26 diet yielding the most optimal results. This study provides valuable insights for sustainable aquaculture feed optimization.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"42 ","pages":"Article 102810"},"PeriodicalIF":3.2,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143848324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Available phosphorus requirement of juvenile Asian red-tailed catfish (Hemibagrus wyckioides)","authors":"Chenghao Yang ,&nbsp;Wenhao Long ,&nbsp;Weijia Cai ,&nbsp;Yaozhengtai Zhang ,&nbsp;Xinping Li ,&nbsp;Yi Hu ,&nbsp;Lei Zhong ,&nbsp;Jihong Dai","doi":"10.1016/j.aqrep.2025.102818","DOIUrl":"10.1016/j.aqrep.2025.102818","url":null,"abstract":"<div><div>This study explored the optimal dietary available phosphorus (P) requirement for juvenile Asian red-tailed catfish (<em>Hemibagrus wyckioides</em>). Five isonitrogenous (42.00 %) and isolipidic (9.00 %) diets with monocalcium phosphate supplementation levels of 0.00 %, 0.75 %, 1.50 %, 2.25 %, and 3.00 % (namely P0, P0.75, P1.5, P2.25, and P3) were fed to fish for 56 d. Weight gain rate increased with phosphorus supplementation up to 2.25 % and then declined. The apparent digestibility coefficients (ADC) of phosphorus and calcium, as well as the phosphorus and calcium contents in the whole body and vertebrae, exhibited a positive correlation with dietary phosphorus levels, while the expression of intestinal genes related to phosphorus transport and metabolism showed a negative correlation with increasing dietary phosphorus levels. Using quadratic regression, the optimal phosphorus requirement was estimated at 0.87<!--> <!-->% based on weight gain and 0.99 % based on vertebrae phosphorus content. Whole body crude lipid content and serum total cholesterol was significantly lower in P1.5, P2.25, and P3 compared to the P0 and P0.75 group (<em>P &lt; 0.05</em>). Liver lipid droplet volume and number were markedly reduced in P2.25 compared to the P0 group. Phosphorus deficiency resulted in stunted growth, reduced bone mineralization, and increased lipid accumulation in whole-body tissue and the liver. These findings provide practical guidance for phosphorus supplementation in <em>H</em>. <em>wyckioides</em> aquaculture, optimizing growth and bone mineralization while minimizing excess phosphorus in feeds.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"42 ","pages":"Article 102818"},"PeriodicalIF":3.2,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143844227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PNPLA3 in largemouth bass (Micropterus salmoides): Molecular cloning, functional characterization, and its role in high glucose-induced lipid synthesis","authors":"Yan-Bin Yang ,&nbsp;Ying-Ying Xu ,&nbsp;Hao Ding ,&nbsp;Jia-Min Ma ,&nbsp;Qing-Hui Ai ,&nbsp;Lei Zhou ,&nbsp;Chao Xu","doi":"10.1016/j.aqrep.2025.102821","DOIUrl":"10.1016/j.aqrep.2025.102821","url":null,"abstract":"<div><div>This study aimed to characterize the full-length cDNA of <em>pnpla</em>3 in <em>Micropterus salmoides</em>, and evaluate its role in high glucose-induced lipid synthesis. The full-length cDNA of <em>pnpla</em>3 was composed of 3366 nucleotides that encoded 550 amino acids. The molecular weight of Pnpla3 was 60.41044 kDa. Pnpla3 protein contained one I-superfamily conotoxin signature, one patatin domain, one (G/A) xGxxG nucleotide domain, one GxSxG lipase motif, one DGG lipase motif, one Ser47-Asp166 catalytic dyad motif, one brummer box domain, and one 148I residue. The high mRNA levels of <em>pnpla</em>3 were got in perivisceral fat followed by liver, white muscle, brain and intestine. High carbohydrates diets and glucose administration resulted in a significant up-regulation of <em>pnpla</em>3 mRNA levels in the liver and muscle. In the <em>in vitro</em> study, PNPLA3 gene silencing significantly decreased <em>pnpla</em>3 mRNA levels and its protein content, and lipid droplets area in hepatocytes. Isoleucine treatment reduced <em>pnpla</em>3 mRNA levels, total triglyceride and cholesterol contents, and lipid droplets area in hepatocytes subjected to high glucose. Overall, <em>M. salmoides pnpla</em>3 is highly conserved, and <em>pnpla</em>3 involved in the regulation of glucose metabolism. Isoleucine could alleviate high glucose-induced lipid accumulation in <em>M. salmoides</em>; this might be achieved through the down-regulation of <em>pnpla</em>3 expression.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"42 ","pages":"Article 102821"},"PeriodicalIF":3.2,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143839109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anesthesia of Baryancistrus xanthellus (Siluriformes: Loricariidae): Bioassays with exposure and re-exposure cause reverse tolerance to eugenol","authors":"T.P. Nascimento ,&nbsp;F.J.T. Barros ,&nbsp;O.L.P. Couto ,&nbsp;M.D.R. Paz ,&nbsp;S.V.N. Junior ,&nbsp;L.M. Sousa ,&nbsp;T.S. Pereira","doi":"10.1016/j.aqrep.2025.102807","DOIUrl":"10.1016/j.aqrep.2025.102807","url":null,"abstract":"<div><div><em>Baryancistrus xanthellus</em> (Golden Nugget Pleco) is an ornamental catfish endemic to the Xingu River basin in Brazil and widely exported for the aquarium trade. This study evaluated the effectiveness of eugenol anesthesia in <em>B. xanthellus</em> under two scenarios: an initial exposure and a re-exposure to the same anesthetic. The experiments were conducted in two phases, two months apart, each testing incremental concentrations of eugenol (2 mg·L⁻¹ added at two-minute intervals) until reaching surgical anesthesia. Two control groups (water only and 70 % ethanol at equivalent concentrations) were subjected to identical handling and observation procedures. Data were analyzed using Permutational Analysis of Variance (PERMANOVA), treating anesthesia stage as the dependent variable, eugenol concentration as the independent variable, and fish size plus exposure history as covariates. The results revealed reverse tolerance to eugenol: fish re-exposed to the anesthetic required significantly lower concentrations to achieve surgical anesthesia (14.50 ± 3.20 mg·L⁻¹) than during the initial exposure (21.69 ± 3.38 mg·L⁻¹). However, recovery times did not differ significantly between the first and second exposures. No mortality occurred within 72 hours post-anesthesia in any group, indicating low short-term toxicity. These findings highlight the need to re-evaluate anesthetic protocols for repeated procedures in ornamental catfish. We also propose new reference tables specifically tailored to describe anesthesia and recovery stages in benthic Loricariids. Further investigations into the biochemical and physiological mechanisms underlying reverse tolerance to eugenol are warranted.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"42 ","pages":"Article 102807"},"PeriodicalIF":3.2,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143839108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Responses of largemouth bass (Micropterus salmoides) to chronic alkalinity stress: Survival, growth performance, physiology, and muscle quality","authors":"Xi Shi ,&nbsp;Shijie Yuan ,&nbsp;Wei Peng ,&nbsp;Fengqing Chen ,&nbsp;Xiangwei Liu ,&nbsp;Xueke Ma ,&nbsp;Xiao Ma ,&nbsp;Ruwei Xu ,&nbsp;Khor Waiho ,&nbsp;Xianghui Kong ,&nbsp;Cuiyun Lu ,&nbsp;Xuejun Li","doi":"10.1016/j.aqrep.2025.102802","DOIUrl":"10.1016/j.aqrep.2025.102802","url":null,"abstract":"<div><div>Saline-alkaline water fishery has emerged as a highly promising aquaculture mode in China. To identify potential species suitable for saline-alkaline water aquaculture, this study investigated the effects of different alkalinity stresses (0, 7, 14, 21, 28 mmol/L) on the survival, growth performance, physiology, and muscle quality of largemouth bass <em>Micropterus salmoides.</em> A total of 450 fish (5.58 ± 0.09 g) were randomly allocated to 15 tanks, with three replicates for each group. The experiment lasted for two months. The results indicated that alkalinity stress exhibited no significant impact on the survival rate but significantly reduced growth performance. Compared with the control group, the ALK-28 group exhibited a significantly higher feed conversion ratio and a relatively lower protein efficiency ratio. Physiologically, as alkalinity increased, the concentrations of Na⁺ and Mg²⁺, along with the Na⁺/K⁺-ATPase and Ca²⁺/Mg²⁺-ATPase activities, showed an upward trend. Histologically, alkalinity stress induced some injuries to gills, including gill lamella hyperplasia, abnormal blood cells accumulation, and chloride cells rupture. For muscle nutrition and quality, no significant differences in the nutritional composition and water-holding capacity among treatments were observed. However, ALK-14 significantly enhanced the flavor and nutritional values of amino acids and fatty acids, and ALK-28 significantly improved textural characteristics, including higher hardness, chewiness, and resilience. These results suggested that largemouth bass is a potentially economic candidate species for saline-alkaline water aquaculture because it can survive and show advantages in muscle nutrition and quality under high alkalinity conditions.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"42 ","pages":"Article 102802"},"PeriodicalIF":3.2,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143829816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary taurine supplementation can improve the growth, protein metabolism, immunity and muscle development of larval largemouth bass (Micropterus salmoides)","authors":"Qing Zhou ,&nbsp;Siying Hu ,&nbsp;Jiacheng Yu ,&nbsp;Can Li ,&nbsp;Xi Liu ,&nbsp;Chen Zhou ,&nbsp;Chengmin Zhao ,&nbsp;Kai Luo ,&nbsp;Weihua Gao ,&nbsp;Chenglong Wu","doi":"10.1016/j.aqrep.2025.102817","DOIUrl":"10.1016/j.aqrep.2025.102817","url":null,"abstract":"<div><div>This study aimed to explore the impact of dietary taurine supplementation on the growth, amino acid transport, protein metabolism, immunity, and muscle and skeletal development of larval largemouth bass (<em>Micropterus salmoides</em>). The larvae (initial weight: 367.53 ± 0.05 mg) were fed with six graded levels of dietary taurine (0 % (control group), 0.15 %, 0.3 %, 0.6 %, 1.2 %, and 2.4 %) for 21 days, which was sufficient for the duration of feeding trial of fish larvae. Results indicated that the weight gain rate and specific growth rate (SGR) of larvae showed a quadratic increase after dietary taurine supplementation (<em>P</em> &lt; 0.05), reaching a maximum in the 0.3 % group. The protease activity was significantly enhanced by 0.3 % of dietary taurine (<em>P</em> &lt; 0.05). The crude protein and amino acid content in the larvae were increased with taurine supplementation, peaking at 0.3 % (<em>P</em> &lt; 0.05). The average myofiber diameter was also significantly enhanced in the 0.3 % group (<em>P</em> &lt; 0.05). Compared to the control group, the supplementation of 0.3 % dietary taurine could upregulate the expressions of amino acid transport protein genes (<em>P</em> &lt; 0.05). The TOR pathway was triggered by 0.3 % of dietary taurine. The AAR, autophagy-lysosome, ubiquitin-proteasome, and calpain pathways were inhibited by 0.3 % dietary taurine. The expressions of myocyte proliferation-related genes (such as <em>Pax3a</em>, <em>MHC</em>, <em>MyoD</em>) and MyoD protein, and <em>Mstn</em> were significantly increased and decreased by dietary taurine, respectively (<em>P</em> &lt; 0.05). In addition, the mRNA levels of skeletal development-related genes were upregulated. Furthermore, dietary taurine supplementation upregulated anti-inflammatory and anti-apoptotic gene expressions, and reduced pro-inflammatory and apoptotic factor expressions in the liver. In summary, dietary taurine enhanced intestinal protein digestion and amino acid absorption, facilitated muscle protein deposition and myofiber development, and improved immunity and skeletal growth, thereby promoting growth in larval largemouth bass. Regression analysis based on the SGR revealed that the optimal taurine supplementation level was 0.288 %.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"42 ","pages":"Article 102817"},"PeriodicalIF":3.2,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143835102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Composition of California’s aquaculture industry and surveying its disease challenges and management strategies","authors":"Alex Wright,&nbsp;Carly Moody,&nbsp;Jackson Gross","doi":"10.1016/j.aqrep.2025.102799","DOIUrl":"10.1016/j.aqrep.2025.102799","url":null,"abstract":"<div><div>California (CA) aquaculture is best characterized by its diversity of production systems, cultured species, and final products. The industry comprises four major sectors (i.e., finfish, shellfish, macroalgae, and natural resource agency), which raise aquatic organisms for a variety of purposes, such as sustenance, conservation, recreation, and scientific research. In terms of commercial production value, California aquaculture contributes roughly 6 % to the total value of the U.S. aquaculture industry. The predominant forms of commercial aquaculture in CA are the production of finfish and shellfish for human consumption, which contribute 40.2 % and 30.0 %, respectively, to the total production value of the industry. Other forms of commercial aquaculture in CA include the production of sportfish species for recreational fishing, and other seafood products like caviar and macroalgae. Natural resource agencies, such as the California Department of Fish and Wildlife (CDFW), are also significant aquaculture producers that raise salmonids for conservation and recreation purposes. The diversity within CA aquaculture requires an equally diverse set of disease management strategies that address the specific needs of each sector. A recent survey on aquatic animal health indicates that California finfish and shellfish facility managers are aware of disease challenges and use a preventative approach to reduce the likelihood of disease outbreaks. Our survey results suggest that when disease is detected, non-prescription antimicrobial products (i.e., hydrogen peroxide) are used most frequently for disease treatment (71 % commercial, 17 % commercial shellfish, 86 % CDFW), while prescription antibiotics are only utilized following veterinary review and approval (18 % commercial finfish, 0 % commercial shellfish, 71 % CDFW). Biosecurity and disease-response protocols are also important components of disease management, and their standardization across each sector will enhance the disease prevention capabilities of the aquaculture industry.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"42 ","pages":"Article 102799"},"PeriodicalIF":3.2,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143835101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Growth performance, flesh fatty acid profile and transcriptome analyses of hybrid Jinhu grouper (Epinephelus fuscoguttatus ♀ × Epinephelus tukula ♂) cultured in Deeper-Offshore Aquaculture System","authors":"Ziyi Wu ,&nbsp;Wenjie Lai ,&nbsp;Qinxi Dai ,&nbsp;Zhenjiang Zou ,&nbsp;Yuyou Lu ,&nbsp;Jiachang Xu ,&nbsp;Yakang Song ,&nbsp;Jingpeng Hou ,&nbsp;Haoran Lin ,&nbsp;Yong Zhang ,&nbsp;Jin Zhang ,&nbsp;Danqi Lu","doi":"10.1016/j.aqrep.2025.102794","DOIUrl":"10.1016/j.aqrep.2025.102794","url":null,"abstract":"<div><div>Amid the rapid growth of offshore aquaculture in China, a Deeper Offshore Aquaculture System (DOAS) for hybrid Jinhu groupers (<em>Epinephelus fuscoguttatus</em>♀× <em>Epinephelus tukula</em>♂) has been established in Guangdong. This study aims to elucidate the biological and metabolic differences between Jinhu groupers cultured in Recirculating Aquaculture Systems (RAS) and those in DOAS. A three-month growth comparison experiment was conducted to assess the developmental differences of Jinhu groupers. This research reveals significant variations in growth performance and flesh fatty acid composition between fish from these two systems, particularly in the content of eicosapentaenoic acid and nine other fatty acids. Through transcriptome analysis of liver and muscle tissues, 278 and 186 differentially expressed genes were identified, respectively, highlighting notable metabolic shifts. Key pathways affected include lipid metabolism and muscle fiber growth. These insights deepen the understanding of the acclimation mechanisms of Jinhu groupers to diverse aquaculture settings and offer valuable strategies for optimizing deeper offshore aquaculture practices, enhancing the sustainability and efficiency of the industry.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"42 ","pages":"Article 102794"},"PeriodicalIF":3.2,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143825539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary lauric acid improves the protein utilization and muscle growth by promoting intestinal flora and glycolysis in mud crab (Scylla paramamosain)","authors":"Wenhao Zhan ,&nbsp;Shichao Xie ,&nbsp;Yao Deng ,&nbsp;Hongyu Peng ,&nbsp;Haiqing Cao ,&nbsp;Zheng Tang ,&nbsp;Yinqiu Tian ,&nbsp;Yu Zhang ,&nbsp;Tingting Zhu ,&nbsp;Min Jin ,&nbsp;Qicun Zhou","doi":"10.1016/j.aqrep.2025.102816","DOIUrl":"10.1016/j.aqrep.2025.102816","url":null,"abstract":"<div><div>At present, the rapid development of aquaculture is restricted by the shortage of fishmeal, and it is particularly important to find ways to replace and reduce fishmeal. It is worth exploring whether feed with lower-than-normal protein requirements have adverse effects on the growth of aquatic animals. The aim of the present study was to improve the growth performance of mud crab (<em>Scylla paramamosain</em>) by adding lauric acid (LA) and glycerol mono-laurate (GML) to a low protein diet (41.8 % crude protein). Adding 1 g/kg LA or 0.5 g/kg GML to low protein diet can significantly ameliorate the negative effects of low protein diet on mud crabs, and improve the survival and growth performance. In addition, dietary LA or GML supplementation can enhance protein apparent digestibility (ADP), increase protein and amino acid content in muscle, and optimize protein metabolism process. Notably, dietary LA and GML supplementation can activate the glycolytic pathway in muscles and up-regulate the expression of genes related to muscle growth. Moreover, the results of intestinal flora indicated that dietary GML and LA increased the abundance of Rhodobacteraceae, Erysipelotrichaceae, Endozoicomonadaceae and <em>Ruegeria</em>. These flora exhibited a positive correlation with protein digestion, absorption and deposition. In conclusion, the supplementation of LA or GML in low-protein diets can effectively improve growth performance, activate muscle glycolysis pathway, promote muscle development, increase microbial abundance related to protein metabolism, and improve protein apparent digestibility.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"42 ","pages":"Article 102816"},"PeriodicalIF":3.2,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143829815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fasting and re-feeding effects for the new measurement to improve feed efficiency traits in Pacific abalone (Haliotis discus hannai)","authors":"Ziheng Yin ,&nbsp;Wenchao Yu ,&nbsp;Junyu Liu ,&nbsp;Jianzhong Weng ,&nbsp;Jie Huang ,&nbsp;Miaoqin Huang ,&nbsp;Jinwei Ke ,&nbsp;Xuan Luo ,&nbsp;Caihuan Ke ,&nbsp;Weiwei You","doi":"10.1016/j.aqrep.2025.102793","DOIUrl":"10.1016/j.aqrep.2025.102793","url":null,"abstract":"<div><div>To speed up the process of measuring abalone for feed efficiency traits and apply the technique in commercial breeding programs, we developed a new evaluation index by fasting and re-feeding treatment. A total of 400 ten-month-old Pacific abalone (<em>Haliotis discus hannai</em>) were randomly sampled. To evaluate feed efficiency traits, each abalone was cultured for 72 days using the previously established single-individual system. After that, the FER was ranked after removing outliers, the top and bottom 150 individuals were exposed to different durations of fasting stress (3, 7, 10, 14, and 21 days), which was followed by single-individual re-feeding treatments. The fluctuations in body weight of each abalone during this process were recorded. The difference in the ability to cope with fasting stress and re-feed between the high- and low-FER (feed efficiency ratio) groups was analyzed. The findings demonstrated that the specific weight loss rate (SWLR) of the high- and low-FER groups of Pacific abalone differed after varying durations of fasting. The results of the correlation analysis between FER and weight change index showed that SWLR was negatively correlated with FER during stress, and the correlation of the 7-day fasting group (S7) was the highest (r = –0.71). The “rapid growth stage” during re-feeding was positively correlated with FER, and the correlation between the body weight gain (BWG) of days 14<strong>–</strong>28 and FER in the 10-day fasting group (S10) was the highest (r = 0.79). The results indicate that there is significant variation in the capacity of abalone to resist fasting stress and re-feeding, and this is associated with feed efficiency. The above measured indicators (SWLR of the S7 group and BWG14–28 of the S10 group) may be used as indirect indicators for feed efficiency traits, and these indicators have application value in abalone breeding.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"42 ","pages":"Article 102793"},"PeriodicalIF":3.2,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143828956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}