Aquaculture Reports最新文献

{"title":"Long-term effects of high carbohydrate feeding in Nile tilapia broodstock on intermediary and carbohydrate metabolism in offspring through juvenile stage","authors":"Linli Luo ,&nbsp;Sirijanya Thongchaitriwat ,&nbsp;Shenglin Yang ,&nbsp;Lucie Marandel ,&nbsp;Stephane Panserat ,&nbsp;Surintorn Boonanuntanasarn","doi":"10.1016/j.aqrep.2025.102941","DOIUrl":"10.1016/j.aqrep.2025.102941","url":null,"abstract":"<div><div>Hyperglucidic stimulation involving high-carbohydrate (CHO) feeding in broodstock could be a practical tool for achieving nutritional programming (NP) in fish. Therefore, this study aimed to investigate the effects of high-CHO (HC) feeding stimuli on reproductive performance, and intermediary CHO metabolic responses in broodstock and its long-term impact on CHO and related metabolism in offspring. Two different CHO diets, HC/low-protein (LP) and low-CHO/high-protein (LC/HP), were fed to mature female and male Nile tilapia. The HC/LP diet in female broodstock led to decreased egg weight, but increased fecundity and gonadosomatic index compared with that with the LC/HP diet. In females and males, blood metabolites, hepatic and muscular compositions, and CHO and its related metabolic responses at the molecular level reflected HC feeding. Similar CHO intermediary metabolic responses were found in offspring at 7-days post-hatching and 7 days after the first feeding, suggesting that the parental effects of CHO intermediary metabolism could be transmitted to offspring at early developmental stages. Modulation of intermediary CHO metabolic responses persisted in the juvenile offspring, with increased hepatosomatic index and hepatic triglyceride levels, muscular glycolysis induction, and suppression of hepatic gluconeogenesis and amino acid catabolism. Juvenile offspring fed the HC diet also had more pronounced intermediary CHO metabolism, including glycogenesis, lipogenesis, glycolysis induction, muscular glucose transport, and suppression of hepatic gluconeogenesis and amino acid catabolism. Collectively, the HC diet-mediated hyperglucidic stimuli in broodstock induced NP effects in offspring, which persisted through the juvenile stage.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"43 ","pages":"Article 102941"},"PeriodicalIF":3.2,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144469930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel phage phaST4: Characterization and its protective potential against Aeromonas hydrophila infection in swamp eels","authors":"Jianfen Wu ,&nbsp;Lifei Chen ,&nbsp;Sixuan Wu,&nbsp;Yi Li,&nbsp;Yuhe Zhao,&nbsp;Chongyang Lu,&nbsp;Wenxiu Sun,&nbsp;Wei Li","doi":"10.1016/j.aqrep.2025.102954","DOIUrl":"10.1016/j.aqrep.2025.102954","url":null,"abstract":"<div><div>The rise in antibiotic resistance of <em>Aeromonas hydrophila</em> has attracted increased concern and attention. In the present study, we isolated and characterized a novel phage designated phaST4 from aquaculture sewage. A morphological analysis revealed that phaST4 exhibited a symmetrical icosahedral head (85.5 ± 2.8 nm) and a long contractile tail (166 ± 3.3 nm × 22 ± 1.3 nm). Whole genome sequence analysis revealed that phaST4 is a double-stranded DNA phage of 90,646 bp in length. Its genome contains 113 putative open reading frames (ORFs) and 4 tRNAs. Moreover, no known virulence genes or drug-resistant genes were identified. Combined with a gene-sharing networks analysis, the results of sequence comparison and phylogenetic analysis indicated that phaST4 is the first representative of a new species within a previously undefined genus. The optimal multiplicity of infection (MOI) for phaST4 was 0.001, and the latent period and burst size were 20 min and 146 plaque-forming units (PFU)/infected cell, respectively. PhaST4 exhibited stable activity at 30°C–50°C and a pH range of 4–11. In addition, it showed a strong tolerance to chloroform. PhaST4 exhibited efficient biofilm inhibition and clearance capacity to its indicator host, as well as time- and MOI-dependent inhibition toward <em>Aeromonas hydrophila</em> in vitro. In addition, intraperitoneal injection of phaST4 significantly reduced the expression of inflammation-related genes, including NF-kB, IL-1β, and IL-6, in the liver and intestine of swamp eels challenged with <em>A. hydrophila</em>. A histopathological analysis revealed that phaST4 administration ameliorates liver and intestinal injury compared with the bacterial infection groups. Overall, the results indicate that phaST4 has potential use for the treatment of <em>A. hydrophila</em> infection.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"43 ","pages":"Article 102954"},"PeriodicalIF":3.2,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144469933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of replacing fishmeal with 70 cottonseed protein concentrate on growth performance, liver health, muscle texture, intestinal microbiome, and wastewater parameters in hybrid grouper (Epinephelus fuscoguttatus♀ × E. lanceolatus♂) juvenile","authors":"Kangze Xv ,&nbsp;Adinda Luthfiah ,&nbsp;Min Xue ,&nbsp;Hao Wang ,&nbsp;Tingting Wang ,&nbsp;Xin Zhang ,&nbsp;Dengbao Fan ,&nbsp;Beiping Tan ,&nbsp;Wei Zhang","doi":"10.1016/j.aqrep.2025.102937","DOIUrl":"10.1016/j.aqrep.2025.102937","url":null,"abstract":"<div><div>As a superior protein historically applied in aquaculture, cottonseed protein has been processed by various deep processing methods to improve its utilization in aquafeeds. In this study, 70 cottonseed protein concentrate with low-gossypol (70 C) was used to replace fishmeal (20 %, 40 %, 60 %, and 80 %) to evaluate the effect on hybrid grouper (<em>Epinephelus fuscoguttatus</em>♀ <em>× E. lanceolatus</em>♂) juveniles (15.00 ± 0.05 g). After a 7-week feeding trial, 40 % 70 C replacing fishmeal had no negative impact on the growth performance of juveniles (<em>P</em> &gt; 0.05). Serum biochemical indices, including alanine aminotransferase (ALT), albumin (ALB), and total cholesterol (TC), were significantly reduced with an increase in dietary 70 C (<em>P</em> &lt; 0.05), suggesting that liver function was impaired, which was further confirmed by the alteration of hepatic morphology. Moreover, the core microbiota of the intestine were majorly Proteobacteria, Firmicutes, Cyanobacteria, Bacteroidota, and Actinobacteriota, etc. at the Phylum level. At the Genus level, <em>Enterobacter</em> significantly decreased with increasing dietary 70 C (<em>P</em> &lt; 0.05). <em>NS3a_marine_group</em>, <em>Sphingomonas Amaricoccus</em>, <em>Craurococcus_Caldovatus</em>, and <em>Agrococcus</em> achieved the highest abundance, whereas <em>Nocardioides</em> and <em>Lachnoclostridium</em> had the lowest abundance in the 70 C20 group. Furthermore, the abundance of <em>Photobacterium</em> and <em>Amaricoccus</em> was more overrepresented in the 70 C60 group than in the other two groups (<em>P</em> &lt; 0.05). The abundance of the predicted pathways related to butanoate metabolism increased in the 70 C group compared to the FM group. Thus, we hypothesized that dietary 70 C would influence the composition of the intestinal microbiota, thereby affecting flesh texture and degrading water quality. Our research provides a theoretical basis for widening the application of cotton protein concentrates in grouper aquafeeds.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"43 ","pages":"Article 102937"},"PeriodicalIF":3.2,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144365626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of β-1,3-glucan feed supplementation on the growth, liver biochemistry, transcript levels and intestinal microorganisms of swamp eel (Monopterus albus)","authors":"Mengyu Bao ,&nbsp;Wenzong Zhou ,&nbsp;Tian Zhu ,&nbsp;Weiwei Huang ,&nbsp;Quan Yuan ,&nbsp;Hang Yang ,&nbsp;Yuning Zhang ,&nbsp;Weiwei Lv ,&nbsp;Qichen Jiang","doi":"10.1016/j.aqrep.2025.102942","DOIUrl":"10.1016/j.aqrep.2025.102942","url":null,"abstract":"<div><div>This study investigated the effects of β-1,3-glucan feed supplementation on the growth, liver biochemistry, transcript levels, and intestinal microbiome of <em>Monopterus albus</em>. The experiment involved five groups: 0 % (CT), 0.2 % curdlan (EG-1), 0.1 % β-1,3-glucan (EG-2), 0.2 % β-1,3-glucan (EG-3), and 0.5 % β-1,3-glucan (EG-4). <em>Monopterus albus</em> were reared for 56 days, the final body weight, weight gain rate, survival rate, and specific growth rate were significantly increased, while the feed conversion rate was reduced considerably, in EG-2, EG-3, and EG-4 compared with the CT (<em>P</em> &lt; 0.05). The addition of β-1,3-glucan significantly reduced the malondialdehyde content (<em>P</em> &lt; 0.05), and significantly increased the activities of superoxide dismutase, catalase, and glutathione peroxidase in the liver (<em>P</em> &lt; 0.05). This indicated that β-1,3-glucan can effectively promote the growth and antioxidant capacity of <em>M. albus</em>. The results of liver tissue sections showed that β-1,3-glucan effectively reduced lipid accumulation in the liver. Transcriptome sequencing revealed that differentially expressed genes were significantly enriched in the peroxisome proliferator-activated receptor signaling pathway, indicating that β-1,3-glucan can regulate liver metabolic functions of <em>M. albus</em>. In addition, intestinal microbial assays showed that dietary β-1,3-glucan decreased intestinal microbial abundance and diversity. These results suggest that the β-1,3-glucan used in this study may reduce microbial abundance and diversity, potentially through the inhibition of certain harmful bacterial species. Furthermore, it increased the relative abundance of the beneficial bacteria <em>Shewanella xiamenensis</em> and the Lachnospiraceae NK4A136 group, which can alter intestinal microbial composition and maintain intestinal health. In summary, these results further elucidate the effects of β-1,3-glucan on the growth, liver antioxidant function and lipid metabolism, and intestinal microorganisms of <em>M. albus</em>. They can facilitate molecular mechanism analysis for the research and development of aquafeed functional additives, which is of great importance for the promotion of green and sustainable aquaculture.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"43 ","pages":"Article 102942"},"PeriodicalIF":3.2,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144365617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative in vitro effects of novel and conventional parasiticides on Thaparocleidus vistulensis (Siwak, 1932) (Monopisthocotyla) parasitizing European catfish (Silurus glanis)","authors":"Wan Muhammad Hazim Wan Sajiri ,&nbsp;Csaba Székely ,&nbsp;István Czeglédi ,&nbsp;Kurt Buchmann ,&nbsp;Boglárka Sellyei","doi":"10.1016/j.aqrep.2025.102952","DOIUrl":"10.1016/j.aqrep.2025.102952","url":null,"abstract":"<div><div>This <em>in vitro</em> study compares the effects of the novel biological parasiticide Biokos (BIO) to the conventional anthelmintics Praziquantel (PZQ) and Mebendazole (MBZ) on the gill parasitic monopisthocotylan <em>Thaparocleidus vistulensis</em> (Siwak, 1932), a species-specific parasite of European catfish (<em>Silurus glanis</em>). Parasites were exposed to long-term treatment and tested at concentrations of 0, 1, 5, 10, 20, 40, 60, 80, and 100 mg/L. The antiparasitic effects were observed at all life cycle stages, including eggs, oncomiracidia, developing juvenile, and adult monopisthocotylans. <em>In vitro</em> assays demonstrated MBZ to be the most effective in inhibiting the development of eggs with 100 % efficacy. Treatment of BIO was proven effective in targeting the oncomiracidia life stage compared to the others. Higher concentrations are required for all antiparasitic treatments to show their effectiveness against developing juvenile and adult <em>T. vistulensis</em>. This suggests that strategic combinations of the parasiticides may elevate efficacy.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"43 ","pages":"Article 102952"},"PeriodicalIF":3.2,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144469931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of replacing fish oil with shrimp waste, canola, and camelina oils on growth and fillet lipid quality in juvenile rainbow trout fed low-fishmeal diets","authors":"Rosendo L. Azcuy ,&nbsp;Adrián J. Hernández ,&nbsp;Lorenzo Márquez ,&nbsp;Matías E. Casaretto ,&nbsp;Gabriel A. Morales ,&nbsp;Patricio Dantagnan","doi":"10.1016/j.aqrep.2025.102956","DOIUrl":"10.1016/j.aqrep.2025.102956","url":null,"abstract":"<div><div>This study assessed the replacement of fish oil (FO) with camelina oil, canola oil, and Argentinian marine shrimp oil (from industry waste) in juvenile rainbow trout (<em>Oncorhynchus mykiss</em>) diets. Fish (initial weight 7.97 ± 0.83 g) were distributed into eighteen 100-liter tanks (36 fish per tank; initial density ∼2.9 kg/m³) and fed six experimental diets for 60 days at 3 % body weight per day. The diets were isonitrogenous (52 % crude protein) and isolipidic (21–22 % lipids). The experimental diets were FO (100 % fish oil), SH (100 % shrimp oil), SHCA (75 % canola + 25 % shrimp oil), COCA (75 % canola + 25 % camelina oil), CO (100 % camelina oil), and CA (100 % canola oil). Growth performance showed comparable outcomes across treatments. Whole-body proximal composition revealed stable dry matter content (96.05 % FO to 97.56 % SHCA) and protein content (50.25 % CO to 52.92 % FO). Lipid content was higher in SHCA (37.36 %) and COCA (37.28 %) diets, while FO (35.03 %) and SH (34.63 %) diets had lower levels. Protein was most digestible in FO (92.6 ± 0.56 %) and SHCA (92.5 ± 0.58 %) diets. Phosphorus digestibility was highest in SH-fed fish (69.7 ± 2.24 %). Fatty acid profiles showed higher saturated fatty acids (SFA) in FO and SH (33.43 and 27.36 mg g⁻¹, respectively) and long-chain omega-3s (EPA and DHA) in FO. CO and CA diets were rich in monounsaturated fatty acids (MUFA), with CA having 57.59 mg g⁻¹ . Fillet nutritional quality indices showed that FO (0.602 ± 0.005) and SH (0.586 ± 0.105) had the highest atherogenicity index and more favorable omega-6/omega-3 ratios (SH: 0.230 ± 0.023, FO: 0.267 ± 0.023).</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"43 ","pages":"Article 102956"},"PeriodicalIF":3.2,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144469932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First insights into Weissella tructae infections in rainbow trout (Oncorhynchus mykiss) in Vietnam: Bacterial characterization, pathogenicity and antimicrobial susceptibility","authors":"Doan Thi Nhinh ,&nbsp;Dang Thi Hoa ,&nbsp;Tran Thi Trinh ,&nbsp;Mai Van Tung ,&nbsp;Nguyen Thi Huong Giang ,&nbsp;Kim Van Van ,&nbsp;Truong Dinh Hoai","doi":"10.1016/j.aqrep.2025.102955","DOIUrl":"10.1016/j.aqrep.2025.102955","url":null,"abstract":"<div><div>Weissellosis has emerged as a significant disease impacting farmed rainbow trout (<em>Oncorhynchus mykiss</em>), resulting in high global mortality rates. In the summer of 2023, an outbreak in Northern Vietnam's rainbow trout farms was linked to Weissellosis, with mortality rates ranging from 20 % to 60 %, prompting an investigation into the causative agent. A total of 106 diseased fish exhibiting panophthalmitis and hemorrhagic eyes were collected from 25 affected farms. Twenty-five representative isolates, each sourced from a different farm, were identified as <em>Weissella tructae</em> through phenotypic and biochemical characterisation, PCR, and 16S rRNA sequencing. Challenge experiments indicated a relatively high virulence of <em>W. tructae</em> in rainbow trout weighing 35–40 g, with LD₅₀ values estimated at 1.2 × 10⁴ CFU/fish. Histopathological examination revealed significant tissue damage, including hemorrhages in the brain, spleen, and kidney. Antimicrobial susceptibility testing of eight antibiotics authorized for use in Vietnam showed the lowest minimum inhibitory concentration (MIC) values for Amoxicillin, Amoxicillin-Clavulanic Acid, and Erythromycin (0.06–1 mg/L). In contrast, most isolates exhibited MIC values of ≥ 512 mg/L for Sulfamethoxazole/Trimethoprim. This study represents the first report of Weissellosis in Vietnamese rainbow trout, highlighting the geographic expansion of the disease and underscoring the urgent need for effective strategies to prevent and control this pathogen.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"43 ","pages":"Article 102955"},"PeriodicalIF":3.2,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144365610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of music enrichment on the welfare of small ornamental fishes","authors":"Tianyu Niu,&nbsp;Bochun Xiang,&nbsp;Puyi Huang,&nbsp;Xigui Yang,&nbsp;Longhui Chai","doi":"10.1016/j.aqrep.2025.102950","DOIUrl":"10.1016/j.aqrep.2025.102950","url":null,"abstract":"<div><div>Auditory enrichment is an emerging method to enhance the welfare of ornamental fish, yet its effects remain poorly understood. This study evaluated how music style and tempo influence stress-related behaviors and cortisol secretion in two model species: zebrafish (<em>Danio rerio</em>) and guppies (<em>Poecilia reticulata</em>). Fish (n = 4 × 3 per species) were exposed for five consecutive days (4 h/day) to one of four auditory treatments—slow-tempo piano (SP, 40–65 bpm), fast-tempo piano (FP, 150–210 bpm), slow-tempo guzheng (SG), fast-tempo guzheng (FG)—or to silence (control). Positive behaviors (touching the water surface, following, wandering) and negative behaviors (freezing, aggression, sprinting) were quantified from 15-min video recordings. Whole-body cortisol concentrations were measured using ELISA. Music enrichment significantly affected both behavioral responses and cortisol levels (ANOVA, p &lt; 0.05). In zebrafish, SP and SG promoted positive behaviors, whereas FP and FG increased negative behaviors; SG alone significantly lowered cortisol relative to the control. In guppies, FP, SG, and FG increased positive behaviors; SG reduced negative behaviors; and all music treatments significantly decreased cortisol levels. Novel tank test performance was unaffected in either species. Overall, slow-tempo guzheng music exerted the strongest anxiolytic effect, particularly in guppies. We conclude that playback of slow guzheng music offers a practical, non-invasive approach to reduce stress and improve welfare in ornamental fish housed in barren aquaria.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"43 ","pages":"Article 102950"},"PeriodicalIF":3.2,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144365618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emerging anthropozoonotic threat in amphibian aquaculture: First report of Trichosporon asahii-induced epidemic with cross-species virulence","authors":"Hongbing Wang ,&nbsp;Guangwei Kuang ,&nbsp;Jie Zhang ,&nbsp;Yao Tian ,&nbsp;Yazhou Hu ,&nbsp;Sha Wang ,&nbsp;Xiaoming Tang ,&nbsp;Yanxia Jiang ,&nbsp;Lifei Du ,&nbsp;Jiaxing Wei ,&nbsp;Junqi Liu","doi":"10.1016/j.aqrep.2025.102948","DOIUrl":"10.1016/j.aqrep.2025.102948","url":null,"abstract":"<div><div><em>Trichosporon asahii</em>, a fungal pathogen predominantly associated with immunocompromised humans, has now been identified as a novel pathogen in amphibian aquaculture. During a 2023 outbreak on a 300,000-individual bullfrog farm in Hunan Province, China, we documented 113,000 mortalities (37.7 % mortality rate) within 60 days, marking the first large-scale epidemic of putrid skin disease in commercial amphibians. Through Koch's postulate fulfillment – isolating seven <em>T. asahii</em> strains from lesions and reproducing disease in laboratory-infected frogs – we confirmed its causative role. Notably, BALB/c murine models revealed systemic infections, demonstrating mammalian virulence potential. These findings redefine <em>T. asahii</em> as a transboundary pathogen requiring coordinated veterinary-medical interventions and provide epidemiological benchmarks for developing biosecurity protocols in intensive frog farming systems.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"43 ","pages":"Article 102948"},"PeriodicalIF":3.2,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144365612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of kelp and fucoidan on lipid metabolism in large yellow croaker (Larimichthys crocea)","authors":"Sifeng Zhu ,&nbsp;Chengwei Huang ,&nbsp;Bin Du ,&nbsp;Rui Yang ,&nbsp;Qijun Luo ,&nbsp;Haimin Chen ,&nbsp;Juanjuan Chen","doi":"10.1016/j.aqrep.2025.102947","DOIUrl":"10.1016/j.aqrep.2025.102947","url":null,"abstract":"<div><div>To investigate the impact of kelp and its bioactive component fucoidan as feed additives on lipid metabolism in large yellow croaker (<em>Larimichthys crocea</em>), an 8-week feeding experiment was conducted using diets supplemented with 3 % kelp (<em>Saccharina japonica</em>, KM group) and 0.2 % fucoidan (FM group). Lipidomic analysis identified over 700 distinct lipids in the muscle, categorized into five main categories and 36 lipid subclasses. Among these, triglycerides (TGs) and phosphatidylcholines (PCs) were the predominant lipid components, with levels in the KM group reduced to 66.34–87.65 % of control values, along with a specific 1.75-fold increase in lysophosphatidylethanolamine (LPE 16:0). Besides, most fatty acids (FAs) demonstrated decreased content in the KM group, except for specific increases in α-linolenic acid (C18:3n-3), arachidonic acid (C20:4n-6), eicosenoic acid (C20:1n-9) and eicosapentaenoic acid (C20:5n-3). The FM group showed both up- and down-regulated trends in lipid content with only a few polyunsaturated fatty acids (PUFAs) showing a decrease. Notably, both kelp and fucoidan supplementation modulated cholesterol metabolism, reducing total and low-density lipoprotein (LDL) cholesterols while increasing high-density lipoprotein (HDL) cholesterol. Moreover, gene expression analysis further supported this finding, showing significant down-regulation of <em>fas</em>, <em>lpl</em>, and <em>srebp1</em> (genes related to FA synthesis) in two groups, while up-regulation of <em>aco</em>, <em>pparα</em>, and <em>pgc-1α</em> (genes promoting β-oxidation) in the KM or FM groups. These results demonstrated that kelp and fucoidan could effectively alter the lipid composition, supporting their potential as sustainable feed additives in aquaculture.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"43 ","pages":"Article 102947"},"PeriodicalIF":3.2,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144365613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}