Aquaculture Reports最新文献

{"title":"Granzyme K provides protection for Nile tilapia (Oreochromis niloticus) from acute bacterial infection.","authors":"Xing Li ,&nbsp;Zhiqiang Zhang ,&nbsp;Baijian Jiang ,&nbsp;Meiling Huang ,&nbsp;Jiamin Feng ,&nbsp;Yongxiong Huang ,&nbsp;Kwaku Amoah ,&nbsp;Berchie Asiedu ,&nbsp;Yu Huang ,&nbsp;Jichang Jian","doi":"10.1016/j.aqrep.2024.102382","DOIUrl":"10.1016/j.aqrep.2024.102382","url":null,"abstract":"<div><div>Granzyme K (GzmK) is a trypsin-like serine protease that functions as a pro-apoptotic protease, has non-cytotoxic functions, and is involved in various physiopathological processes. However, the impact of GzmK on the immunological response of Nile tilapia against bacterial challenge is still poorly explored. In this study, we identified the <em>GzmK</em> gene from Nile tilapia (<em>On-GzmK</em>) and investigated its immunologic roles through in <em>vivo</em> experiments. <em>On-GzmK</em> has an open reading frame of 786 bp and encodes 255 amino acids. On-GzmK has over 60 % genetic similarity with other fish and over 30 % similarity with mammals. Its transcript levels were highest in the liver and vastly increased after being challenged with <em>Streptococcus agalactiae</em> and <em>Aeromonas hydrophila.</em> In <em>vivo</em> experiments implied that On-GzmK might promote inflammatory responses and regulate apoptosis and pyroptosis by participating in the activation of multiple signaling pathways, such as JAK-STAT, MAPK, and NF-κB. The present observations offer additional theoretical support for investigating the processes by which GzmK shields fish against bacterial infections.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102382"},"PeriodicalIF":3.2,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142323254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selection of antibacterial aptamers against Pseudomonas plecoglossicida and analysis on their potential binding proteins","authors":"Yunting Fan ,&nbsp;Xiaojun Lin ,&nbsp;Ying Tan ,&nbsp;Mao Lin ,&nbsp;Ming Fang ,&nbsp;Lixing Huang ,&nbsp;Qingpi Yan ,&nbsp;Deji Gao ,&nbsp;Jiaen Wang ,&nbsp;Qibiao Weng ,&nbsp;Jun Bo ,&nbsp;Jiang Zheng","doi":"10.1016/j.aqrep.2024.102381","DOIUrl":"10.1016/j.aqrep.2024.102381","url":null,"abstract":"<div><div><em>Pseudomonas plecoglossicida</em> is one of the main pathogens causing visceral white spot disease of the large yellow croaker (<em>Pseudosciaena crocea</em>). Although antibiotics are used to control <em>P. plecoglossicida</em> infections, the long-term and large-scale use of antibiotics can lead to the development of drug-resistant bacteria as well as environmental pollution. Therefore, it is necessary to develop novel antibacterial agents to treat <em>P. plecoglossicida</em> infections. In this study, we first developed a two-step-centrifugation method to isolate live and dead <em>P. plecoglossicida</em> cells. Subsequently, we used the Systematic Evolution of Ligands by Exponential Enrichment (SELEX) screening based on inhibition rate to directly isolate antibacterial aptamers from the random library without post-processing. We isolated five antibacterial aptamers, namely B1, B2, B4, B8, and B109, which showed good inhibitory effects against <em>P. plecoglossicida</em>. Among these, B4 showed the highest inhibitory effect (62.40 ± 4.17 %). Further analysis revealed no positive correlation between the inhibitory effect of aptamers and their affinities with the target bacterium. The B4- and B109-binding proteins were isolated from <em>P. plecoglossicida</em> by magnetic separation and sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE). Mass spectrometry analysis revealed that the 26 kDa ribosomal protein L2 was the probable B4-binding protein, while the 26 kDa ribosomal protein S3 and 75 kDa ribosomal protein S1 or succinate dehydrogenase flavoprotein subunit were the probable B109-binding proteins. Structural and subcellular localization analyses of these potential B4- and B109-binding proteins were also conducted. Our findings suggest that the inhibitory activity of the antibacterial aptamers against <em>P. plecoglossicida</em> may be mediated via their interaction with the ribosomal proteins, which can interfere with the protein synthesis process in the bacterium, affecting its growth. These findings provide the scientific basis for the development of functional antibacterial aptamers and the elucidation of their mechanisms of action.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102381"},"PeriodicalIF":3.2,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142328161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cloning and characterization of apolipoprotein A-IV (ApoA-IV) from Oreochromis niloticus involved in immune defense functions","authors":"Yongxiong Huang ,&nbsp;Xuyan Tan ,&nbsp;Jiamin Feng ,&nbsp;Zhiqiang Zhang ,&nbsp;Kwaku Amoaha ,&nbsp;Berchie Asiedu ,&nbsp;Yu Huang ,&nbsp;Jichang Jian","doi":"10.1016/j.aqrep.2024.102355","DOIUrl":"10.1016/j.aqrep.2024.102355","url":null,"abstract":"<div><div>Apolipoprotein A-IV (ApoA-IV) is a multifunctional protein that participates in a broad spectrum of biological processes, including the modulation of immune function. Nevertheless, the specific roles of ApoA-IV in the immune response to acute bacterial infections in Nile tilapia (<em>Oreochromis niloticus</em>) are yet to be fully elucidated. In the present study, we identified <em>ApoA-IV</em> from <em>O. niloticus</em> (On-ApoA-IV) and examined its role in bacterial infections. The open reading frame of On-ApoA-IV spanned 768 bp and encoded a sequence of 255 amino acids. Its transcriptional abundance was the highest in the intestine, and <em>On-ApoA-IV</em> was induced in tilapia challenged with <em>Streptococcus agalactiae</em>. Additionally, experimental findings indicated that On-ApoA-IV can inhibit inflammatory processes and apoptosis, thereby enhancing tilapia survival during acute bacterial infection. The current data provide a theoretical foundation for further elucidation of the mechanisms by which ApoA-IV safeguard fish against pathogens.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102355"},"PeriodicalIF":3.2,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142324074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of artificial shelter on feeding, digestion, and growth of the sea cucumber Apostichopus japonicus","authors":"Xiang Li,&nbsp;Xiajing Wang,&nbsp;Zihe Zhao,&nbsp;Yingzhuo Sun,&nbsp;Jun Ding,&nbsp;Yaqing Chang,&nbsp;Chong Zhao","doi":"10.1016/j.aqrep.2024.102379","DOIUrl":"10.1016/j.aqrep.2024.102379","url":null,"abstract":"<div><div>Shelters are widely used in sea cucumber aquaculture. But the long-term effects of shelters on behavior and physiology are unknown in sea cucumber aquaculture. In this research, the number of grasping of the sea cucumber mouth tentacle significantly increased in the experimental group (group Y) compared with the control group (group C) (<em>P</em> = 0.003), indicating that the shelter increases the feeding rate of sea cucumbers. The height of the intestinal crease in group Y was significantly higher than that in group C (<em>P</em> &lt; 0.001), and the proportion of Patescibacteria in the intestine significantly increased. However, there was no significant difference in digestive enzyme activities, and the defecation rate of <em>Apostichopus japonicus</em> in group Y was significantly lower than that in group C (<em>P</em> = 0.047). This suggests that the shelter probably improves the digestive capacity of sea cucumbers by altering their gut microbial composition and gut structure. In addition, final body weight (<em>P</em> = 0.006) and daily gain (<em>P</em> = 0.008) in group Y were significantly higher than those in group C. This indicates that the shelter improves the growth efficiency of sea cucumbers. The present study reveals that long-term use of shelter greatly improves feeding behavior, defecation behavior, gut microbiota composition, gut structure, and growth of <em>A. japonicus.</em> In summary, the shelter improves the behavior and physiological activities of sea cucumbers, and consequently increases the aquaculture efficiency.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102379"},"PeriodicalIF":3.2,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142319807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of dietary phospholipids on growth performance, antioxidant capacity, and lipid metabolism of juvenile Chinese sturgeon (Acipenser sinensis), a critically endangered sturgeon in the Yangtze River","authors":"Jinghua Chen ,&nbsp;Chao Sui ,&nbsp;Yacheng Hu ,&nbsp;Huimin Qin ,&nbsp;Danyang Zhang ,&nbsp;Jiang Wei ,&nbsp;Boji Cao ,&nbsp;Qingfei Li","doi":"10.1016/j.aqrep.2024.102366","DOIUrl":"10.1016/j.aqrep.2024.102366","url":null,"abstract":"<div><div>This study aimed to investigate the effects of dietary phospholipids (PLs) on growth performance, antioxidant capacity, and lipid metabolism of juvenile Chinese sturgeon (<em>Acipenser sinensis</em>). Juvenile sturgeon, with an initial body weight of 12.27±0.03 g, were fed five diets containing graded levels of PLs (1.26 %, 3.29 %, 4.34 %, 5.93 %, and 7.02 %) in triplicate for 56 days. All experimental diets were formulated to be isonitrogenous (55 %) and isolipidic (10 %). Results indicated that specific growth rate increased from 2.21 to 2.89 with elevated levels of dietary PLs. The optimum PLs level for juvenile Chinese sturgeon was estimated to be 5.93 % of diet according to the broken-line regression analysis of the specific growth rate against dietary PLs levels. Diets containing higher levels of PLs (5.93 % and 7.02 % of diet) significantly reduced the lipid content of whole body and liver by around 30 % in juvenile sturgeon compared to the control group (<em>P</em> &lt;0.05). The proportions of specific fatty acids present in the liver lipid were linearly correlated with their percentages in dietary lipid (<em>P</em> &lt;0.05), whereas muscle fatty acid composition remained unaffected across different treatments (<em>P</em> &gt;0.05). EPA and DHA were preferentially accumulated into the muscle tissue. Activities of digestive enzymes and antioxidant enzymes were linearly increased with incremental dietary PLs levels (<em>P</em> &lt;0.001). Inclusion of PLs in diet significantly enhanced the expression of antioxidant genes with the transcription of <em>GSH-Px</em> in the liver of fish fed 7.02 % PLs of diet being approximately 8-fold greater than in those fish fed diet without PL addition (<em>P</em> &lt;0.001). Dietary PLs significantly induced the gene expression related to lipid catabolism, and suppressed the transcription of genes involved in lipogenesis (<em>P</em> &lt;0.001). Based on growth performance, antioxidant capacity, and liver biochemical parameters, inclusion of 5.93 % PLs (soy lecithin preparation) in the diets is recommend for juvenile Chinese sturgeon.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102366"},"PeriodicalIF":3.2,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S235251342400454X/pdfft?md5=8f188ce53c67209708065e6b11fe4cb1&pid=1-s2.0-S235251342400454X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142315980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Replacement of dietary fish meal with soy protein concentrate on the growth performance, PI3K/AKT/TOR pathway, immunity of abalone Haliotis discus hannai and its resistance to Vibrio parahaemolyticus","authors":"Xiaojun Yu ,&nbsp;Zhenhua Wu ,&nbsp;Yonghao Fu ,&nbsp;Jinshu Guo ,&nbsp;Kai Luo ,&nbsp;Wanyou Zhou ,&nbsp;Kangsen Mai ,&nbsp;Wenbing Zhang","doi":"10.1016/j.aqrep.2024.102368","DOIUrl":"10.1016/j.aqrep.2024.102368","url":null,"abstract":"<div><div>Five isonitrogenous and isolipidic experimental diets were formulated to investigate the influence of soy protein concentrate (SPC) replacing dietary fish meal (FM) on the growth, PI3K/AKT/TOR pathway, immunity of abalone <em>Haliotis discus hannai</em> and its resistance to <em>Vibrio parahaemolyticus</em>. The basal diet was designed with 20 % of FM. Based on it, the FM was replaced by SPC at 0 (CON), 25 % (SPC25), 50 % (SPC50), 75 % (SPC75) and 100 % (SPC100), respectively. After the 110-day feeding trial, it was shown that completely replacing FM with SPC had no adverse effects on growth, feed utilization and body composition of abalone. The highest weight gain rate of abalone was 119.52 %, which was observed in the SPC25 group. The trypsin activity in the digestive gland within the SPC25 and SPC50 groups exhibited a significant increase compared to that in the remaining groups. Relative to the CON group, the SPC100 group showed a significant reduction in total antioxidant capacity and an increase in malondialdehyde content within the cell-free hemolymph (CFH). The <em>myd88</em> mRNA expression in the SPC25-SPC75 groups was significantly decreased compared with other groups. The highest expressions of <em>pi3k</em>, <em>mtor</em> and <em>s6k</em>, and the lowest expression of <em>4e-bp</em> were found in the SPC25 group. The hardness of muscle was significantly increased with the increasing dietary SPC levels. The CON, SPC25, and SPC50 groups exhibited significantly lower cumulative mortality rates among abalones following the <em>V. parahaemolyticus</em> challenge. In conclusion, in terms of growth, FM can be totally replaced by SPC. Furthermore, replacing 25 % of dietary FM with SPC led to a significant enhancement in abalone growth. Moderately replacing FM with SPC (up to 50 %) could improve abalone digestive capacity and did not significantly affect abalone survival following a <em>V. parahaemolyticus</em> challenge. The optimal replacement level of FM by SPC (≤ 25 %) triggered the PI3K/AKT/TOR pathway to promote protein synthesis. Considering the growth, digestion, immunity, muscle quality and disease resistance of abalone, it is recommended that dietary FM can be replaced by SPC not more than 50 %.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102368"},"PeriodicalIF":3.2,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352513424004563/pdfft?md5=a386e419bc4df07b175799fee4b7c642&pid=1-s2.0-S2352513424004563-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142315981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome analysis provides novel insights into the factors influencing the settlement and metamorphosis of Pinctada maxima","authors":"Minxin Liang ,&nbsp;Wenhao Yu ,&nbsp;Kaiyao He ,&nbsp;Qingheng Wang ,&nbsp;Yongshan Liao ,&nbsp;Ronglian Huang ,&nbsp;Dewei Cheng ,&nbsp;Yuewen Deng ,&nbsp;Zhe Zheng ,&nbsp;Chuangye Yang","doi":"10.1016/j.aqrep.2024.102377","DOIUrl":"10.1016/j.aqrep.2024.102377","url":null,"abstract":"<div><div>The metamorphosis of the planktonic larvae of bivalve species plays a key role in their complex life history strategies, and this process involves changes in the molecular machinery required for sessile life and the development of intricate shells. Understanding the key molecular agents and special requirements in this development stage is critically important for increasing the production of larvae and promoting population recovery. Here, we cultured larvae of the giant pearl oyster <em>Pinctada maxima</em>, a protected bivalve species in China that is used for the commercial production of large pearls. RNA sequencing was used to identify differentially expressed genes (DEGs) before (E) and after metamorphosis (ES). A total of 8270 DEGs were identified. The up-regulation of genes related to oxidative phosphorylation at the ES stage indicate an increase in energy metabolism. The DEGs were systematically determined by conserved domain analysis. DEGs involved in apoptosis and autophagy coincided with changes in morphology and tissue reconstruction at the metamorphosis stage. In addition, the increased expression of genes related to hormone, neurotransmitter/neuropeptide receptor, and immunitye at the ES stage suggests that they play an important role in the induction of various metabolic processes and the development of the immune system following exposure to new environments. The high expression of vitamin B transporters indicates that the absorption and bioavailability of vitamin B are particularly important for the metamorphosis of <em>P. maxima</em>. These findings provide new insights into the factors inducing metamorphosis and the nutritional requirements of rare bivalve species, which have implications for the population restoration and breeding of bivalves.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102377"},"PeriodicalIF":3.2,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352513424004654/pdfft?md5=d41e21cf09cb43c3ec4699edf1f7be98&pid=1-s2.0-S2352513424004654-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142315982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary high plant protein and high lipid impaired the intestine health of grouper by disrupting oxidative stress, immune response, and protein metabolism","authors":"Jia Xu ,&nbsp;Fan Wang ,&nbsp;Chaoqun Hu ,&nbsp;Junxiang Lai ,&nbsp;Shiwei Xie ,&nbsp;Kefu Yu ,&nbsp;Fajun Jiang","doi":"10.1016/j.aqrep.2024.102389","DOIUrl":"10.1016/j.aqrep.2024.102389","url":null,"abstract":"<div><div>This study aimed to investigate the molecular mechanisms underlying the detrimental effects of high lipid (HL) and high plant-protein (HP) diets on gut health in pearl gentian grouper, <em>Epinephelus fuscoguttatus</em>♂ × <em>Epinephelus lanceolatus</em>♀. Thus, grouper were randomly distributed into 12 plastic tanks, which were randomly assigned to four diet groups: control (C, 9.48 % lipid, 46.21 % protein), high lipid (HL, 16.70 % lipid, 46.37 % protein), high plant-protein (HP, 9.38 % lipid, 46.50 % protein), high lipid and high plant-protein (HLP, 16.67 % lipid, 46.54 % protein). After an 8-week feeding trial, the HL and HP treatments led to impaired growth performance, compromised gut structure, suppressed immune components, reduced antioxidant defense through the Keap/Nrf2 signaling, and inhibited protein metabolism via the PI3K/AKT/mTOR signaling. Notably, their combined effects (HLP) further exacerbated it. A significant decrease in the proportion of unclassified_<em>Peptostreptococcaceae</em>, <em>Clostridium</em>, and <em>Curvibacter</em> in all treatment groups. The <em>Streptococcus</em>, <em>Lactobacillus</em>, and <em>Bosea</em> were influenced by the interaction between dietary lipids and plant proteins. 14 metabolites that exhibited significant differences among all treatment groups, including arginine, prostaglandin B1, cortexolone, alpha-linolenic acid, and allocholic acid. The HL treatment primarily affected pathways related to lipid metabolism and immunomodulation, while the HP treatment predominantly influenced pathways associated with protein metabolism and immunomodulation. Our findings also indicated that the HP factor played a more prominent role in shaping the metabolic response of fish to the HLP intervention. Furthermore, a significant positive correlation between the <em>Clostridium</em> and the metabolites Cortexolone and Allocholic acid, while a negative correlation was observed between the <em>Streptococcus</em>, <em>Lactobacillus</em>, and <em>Lactococcus</em> and these metabolites. In conclusion, our study demonstrates that excessive inclusion of plant proteins and/or lipids negatively affects gut health in grouper by disrupting oxidative stress, immune response, and protein metabolism through modulation of the gut microbial-metabolite crosstalk.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102389"},"PeriodicalIF":3.2,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352513424004770/pdfft?md5=f6205ef4eae579545cf6102d1b04789f&pid=1-s2.0-S2352513424004770-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142316254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of background color on the growth performance, digestive enzyme activity, antioxidant capacity, and intestinal microbiota of juvenile Plectropomus leopardus","authors":"Huapeng Chen,&nbsp;Huan Liang,&nbsp;Haoran Yu,&nbsp;Shukui Sun,&nbsp;Da Zheng,&nbsp;Lei Wang,&nbsp;Xinxin Wang,&nbsp;Junlong Sun,&nbsp;Jian Luo,&nbsp;Feibiao Song","doi":"10.1016/j.aqrep.2024.102370","DOIUrl":"10.1016/j.aqrep.2024.102370","url":null,"abstract":"<div><div>The scale of <em>Plectropomus leopardus</em> breeding is continually expanding, and this has necessitated constant changes to ensure that breeding conditions are optimized. Background color is an important factor affecting fish feeding. In this study, fish (10.58 ± 0.03 g) were cultured on five background colors (red, blue, black, white, and transparent), and the growth status and intestinal microbes of these fish over 56 days were analyzed. Growth performance and the expression of growth-related genes were optimized when the background color was white. The white group had higher AMS and LPS enzyme activities and lower T-SOD and T-AOC enzyme activities, indicating that the white background could effectively promote feeding and reduce oxidative stress in <em>P. leopardus</em>. Additionally, changes in background color altered the composition and function of the intestinal microbiota. The diversity and abundance of intestinal microbiota, including the abundance of beneficial bacteria, were highest in fish on a white background. The abundances of Firmicutes and Actinobacteriota and the abundance of <em>Bacillus</em> were higher in the White group than in the other groups, indicating that a white background promotes the health of juvenile <em>P. leopardus</em>. In conclusion, a white background can promote the growth performance of <em>P. leopardus</em> and increase the abundance of beneficial intestinal microbes. Generally, the results of our study could be used to optimize the culture of <em>P. leopardus</em>.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102370"},"PeriodicalIF":3.2,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352513424004587/pdfft?md5=560041453b4566fcb3417ecfea3f6993&pid=1-s2.0-S2352513424004587-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142311199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular identification of a steroidogenesis-related gene, star3, and its function in gonadal development of Hyriopsis cumingii","authors":"Yang Gu ,&nbsp;Xiaozhao Shangguan ,&nbsp;Yingrui Mao ,&nbsp;Weizhong Li ,&nbsp;Yijin Ren ,&nbsp;Zhengxin Li ,&nbsp;Huohong lv ,&nbsp;Yihan Wu ,&nbsp;Guiling Wang","doi":"10.1016/j.aqrep.2024.102375","DOIUrl":"10.1016/j.aqrep.2024.102375","url":null,"abstract":"<div><div><em>Star3</em> is located in sex hormone synthesis signaling pathway associated with gonadal development. In this study, <em>star3</em> was first cloned from the gonad of <em>Hyriopsis cumingii</em>. The cDNA of <em>star3</em> in <em>H. cumingii</em> was 2072 bp in length, concluding an open reading frame (ORF) of 1419 bp, encoding 472 amino acids with a conserved START structural domain. Tissue distribution analysis showed <em>star3</em> highest expressed mainly in gonad, which had higher transcription levels in testis than that in ovary. Significant hybridization signals were detected on the follicle wall cell of male gonad. Gonad expression patterns revealed <em>star3</em> highest expressed at two months old during juvenile stages. After RNA interference with <em>star3</em>, expression levels of <em>cyp17a</em> and <em>17β-hsd11</em> was repressed. Furthermore, contents of pregnenolone, testosterone, and estradiol in gonad were reduced significantly. These results suggest <em>star3</em> may be involved in sex hormone synthesis and play an important role in early gonadal development of <em>H. cumingii</em>.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102375"},"PeriodicalIF":3.2,"publicationDate":"2024-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352513424004630/pdfft?md5=fb5f672508da0feae83abb63401efad6&pid=1-s2.0-S2352513424004630-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142311198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}