Aquaculture Reports最新文献

{"title":"Dietary kelp meal alleviates feed starch intolerance of hybrid snakehead (Channa maculata ♀ × Channa argus ♂) by improving liver glycolipid metabolism and metabolite profile","authors":"Shaodan Wang ,&nbsp;Di Sun ,&nbsp;Chong Wang ,&nbsp;Guohuan Xu ,&nbsp;Jixing Zou","doi":"10.1016/j.aqrep.2025.102741","DOIUrl":"10.1016/j.aqrep.2025.102741","url":null,"abstract":"<div><div>This study aimed to assess the influence of kelp meal (KM) on growth performance, hepatic glycogen accumulation, hepatic lipid deposition, as well as liver glucose-lipid metabolism in hybrid snakehead (<em>Channa maculata</em> ♀ × <em>Channa argus</em> ♂). A total of 810 juvenile hybrid snakeheads (11.4 ± 0.15 g) were randomly allocated into three groups (3 replicates per group) and given isontirogenous and isolipidic diets containing different levels of KM (10 %、15 %) for 60 days. The results showed that KM diets can significantly reduce liver glycogen accumulation and postprandial hyperglycemia in hybrid snakehead. This is accomplished by increasing levels of insulin secretion and decreasing glucagon levels, thereby increasing liver gluconeogenesis and inhibiting glycolysis. Additionally, there is a considerable reduction in liver lipid deposition with the KM diets. This is due to the lipid oxidative decomposition is promoted, and the liver lipid synthesis is inhibited. Meanwhile, KM diets enhance the body's lipid transport system. Omics analysis showed that the key to KM diets improving liver glycolipid metabolism of hybrid snakehead lies in regulating amino acid metabolism and lipid metabolism, and affecting changes in key metabolites of lipid metabolism. Among them, Jasmolone glucoside, L-Methionine S-oxide, Asparaginyl-Isoleucine and Didymin are key metabolites of great significance. Therefore, our study shows that 10 % KM diets can improve the glycolipid metabolism in hybrid snakehead liver, reduce lipid deposition, and alleviate hyperglycemia.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"42 ","pages":"Article 102741"},"PeriodicalIF":3.2,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143592934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of dietary Lactobacillus pentosus supplementation on growth performance, serumbiochemistry and liver function of hybrid grouper (♀ Epinephelus fuscoguttatus × ♂ Epinephelus lanceolatus) fed with oxidized fish oil diet","authors":"Wenshan Cai ,&nbsp;Zhihao Li ,&nbsp;Weibin Huang ,&nbsp;Menglong Zhou ,&nbsp;Hao Liu ,&nbsp;Yuanzhi Yang ,&nbsp;Beiping Tan ,&nbsp;Xiaohui Dong","doi":"10.1016/j.aqrep.2025.102736","DOIUrl":"10.1016/j.aqrep.2025.102736","url":null,"abstract":"<div><div>This study was to investigate the effects of dietary <em>Lactobacillus pentosus</em> (LP) supplementation on the growth performance, serum biochemistry and liver function of hybrid grouper (<em>♀ Epinephelus fuscoguttatus × ♂ E. lanceolatus</em>) fed with oxidized fish oil. Diet containing 9 % fresh fish oil was used as the positive control group (Group F); diet containing 9 % oxidized fish oil was used as the negative control group (Group O), and 1 × 10⁷cfu/g, 1 × 10⁸cfu/g, and 1 × 10⁹ cfu/g of LP were added to Group O, named Group O+ 7 L, O+ 8 L, and O+ 9 L. The results showed that (1) Growth performance of group F and group O+ 9 L were considerably greater than those of group O (P &lt; 0.05). (2) The total cholesterol (CHOL) and high-density lipoprotein (HDL-C) contents in the serum of group F and group O+ 9 L grouper were considerably greater than those of group O, and the low-density lipoprotein (LDL-C) and malondialdehyde (MDA) contents were considerably less than those of group O (P &lt; 0.05). The alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum of group O were considerably greater than those of group F and group O+ 9 L (P &lt; 0.05). (3) Liver nuclear respiratory factor 2 (<em>nrf2</em>), glutathione peroxidase (<em>gpx</em>), catalase (<em>cat</em>), peroxisome proliferator-activated receptor α (<em>pparα</em>), carnitine palmitoyltransferase1 <em>(cpt1</em>), and phosphatase and tensin homolog (<em>pten</em>) expression were up-regulated, and hepatic kelch-like ECH-associated protein 1 (<em>keap1</em>), and peroxisome proliferator-activated receptor γ (<em>pparγ</em>) expression were down-regulated of LP treated group, compared with group O. The results showed that the addition of 1 × 10⁹ cfu/g LP promoted the decomposition lipid and antioxidant capacity of liver, thereby resisting the damage of oxidized fish oil to grouper.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"42 ","pages":"Article 102736"},"PeriodicalIF":3.2,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143578368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary iron oxide (Fe2O3) nanoparticles modulate growth performance, body composition, mineral content and intestinal health of yellow catfish juveniles (Pelteobagrus fulvidraco)","authors":"Xiao-Lei Wei ,&nbsp;Zhi-Wei Hao ,&nbsp;Yannis P. Kotzamanis ,&nbsp;Tian-Hua Zhang ,&nbsp;Zhi-Bo Liu ,&nbsp;Hong Yang ,&nbsp;Zhi Luo","doi":"10.1016/j.aqrep.2025.102739","DOIUrl":"10.1016/j.aqrep.2025.102739","url":null,"abstract":"<div><div>The present study investigated the growth performance, body composition, mineral content and intestinal health of yellow catfish juveniles fed different iron diets and explored the dietary Fe requirements for this fish species. Using iron oxide nanoparticles (Fe₂O₃ NPs) as the Fe sources, we formulated six experimental diets with dietary Fe levels at 22.23, 28.28, 36.10, 44.53, 52.21, and 61.85 mg Fe/kg diet, respectively. Yellow catfish fed with 44.53 mg Fe/kg diet had the highest growth performance and feed utilization, while those fed 61.85 mg Fe/kg diet showed the lowest growth performance and feed utilization. Dietary Fe addition increased whole-body and intestinal Fe contents. Additionally, the addition of dietary Fe had an impact on whole-body crude protein, crude lipid, ash, calcium (Ca), magnesium (Mg), zinc (Zn), and manganese (Mn) contents, but did not whole-body moisture and copper (Cu) contents. Yellow catfish fed with 44.53 mg Fe/kg diet had an improvement in intestinal histology and an increase in expression of intestinal tight junction-related genes [<em>occludin</em> and <em>tight junction protein 1b</em> (<em>zo-1b</em>)] as well as antioxidant response genes [<em>superoxide dismutase 1</em> (<em>sod1</em>), <em>sod2</em> and <em>catalase</em> (<em>cat</em>)], and had higher activities of antioxidant enzymes [CAT, total SOD (T-SOD) and total antioxidant capacity (T-AOC)]. However, malondialdehyde (MDA) content, and gene expression of <em>glucose-regulated protein 78</em> (<em>grp78</em>), <em>eukaryotic translation initiation factor 2α</em> (<em>eif2α</em>), <em>activating transcription factor 4</em> (<em>atf4</em>), <em>inositol-requiring enzyme 1</em> (<em>ire1</em>) (related to endoplasmic reticulum stress), and mRNA levels of <em>tumor necrosis factor α</em> (<em>tnfα</em>), <em>interleukin 1β</em> (<em>il1β</em>), <em>il8</em> (related to inflammatory response) in the intestine were the lowest in 44.53 mg Fe/kg diet group. Conducting broken-line analysis of weight gain (WG) against dietary Fe levels, we revealed that the optimal Fe requirement was 45.28 mg Fe/kg diet, with Fe₂O₃ NPs as Fe source.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"42 ","pages":"Article 102739"},"PeriodicalIF":3.2,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143578472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detecting the marine gastropod Lacuna vincta at seaweed farms using a novel environmental DNA (eDNA) assay","authors":"Cara A. Blaine ,&nbsp;Thew Suskiewicz ,&nbsp;Markus Frederich ,&nbsp;Emily Rose Lancaster ,&nbsp;Carrie J. Byron","doi":"10.1016/j.aqrep.2025.102729","DOIUrl":"10.1016/j.aqrep.2025.102729","url":null,"abstract":"<div><div>The small marine gastropod <em>Lacuna vincta</em> is species of concern for kelp (<em>Saccharina latissima, S. angustissima</em>) farmers and processors because snails attach their egg sacs to the blades of cultivated kelp and are difficult to remove. Larval dispersal patterns and post-metamorphic migratory behavior have allowed <em>L. vincta</em> to encounter and settle on kelp blades suspended from farms. Snail infestations can force farmers to harvest early or redirect biomass into lower-value products. We created and tested a novel species-specific environmental DNA (eDNA) assay for <em>L. vincta</em> throughout two kelp growing seasons in conjunction with traditional visual surveying for snails on farms. We were able to detect and amplify <em>L. vincta</em> DNA from environmental field samples interannually and across multiple sites as well as to link the presence of <em>L. vincta</em> on farms to the presence of <em>L. vincta</em> DNA at farm sites. This and future research will contribute to best management practices for kelp farmers when it comes to choosing lease sites for aquaculture farms and determining when to harvest biomass in the spring.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"42 ","pages":"Article 102729"},"PeriodicalIF":3.2,"publicationDate":"2025-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143578468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reproductive performance of female orange mud crab, Scylla olivacea, based on body size and mating strategies","authors":"Mohammad Asmat-Ullah ,&nbsp;Khor Waiho ,&nbsp;Hanafiah Fazhan ,&nbsp;Saleh Ahmed ,&nbsp;Muyassar H. Abualreesh ,&nbsp;Mohd Husin Norainy ,&nbsp;Sk Ahmad Al Nahid ,&nbsp;Hongyu Ma ,&nbsp;Teoh Hong Peng ,&nbsp;Md Moshiur Rahman ,&nbsp;Mhd Ikhwanuddin","doi":"10.1016/j.aqrep.2025.102718","DOIUrl":"10.1016/j.aqrep.2025.102718","url":null,"abstract":"<div><div>In natural or induced breeding, broodstock size is a critical morphological characteristic for producing aquaculture seeds. As such, the present study determined the effects of female broodstock size on the reproductive performance (spawning, egg fertilisation, hatching success, and interval from eyestalk ablation to spawning and spawning to hatching) of orange mud crabs, <em>Scylla olivacea</em>, in captivity. This study employed female induced-to-mate (IM) and wild-mated (WM) mud crabs with carapace width (CW) &lt; 100 mm and CW ≥ 100 mm to obtain larvae. Post-cautery, unilateral eyestalk ablation (UEA) was conducted to induce spawning of the mated females. The latency period following UEA incorporation was short (9–13 days) in IM females (CW ≥ 100 mm). Meanwhile, the WM females (CW ≥ 100 mm) recorded varying latency period ranges (8–52 days). Only the larger crabs (CW ≥ 100 mm) documented successful hatching (IM: 90.88 ± 0.98 % and WM: 92.16 ± 0.70 %) and superior spawning success (IM: 86.67 ± 11.55 % and WM: 73.33 ± 23.09 %). Nevertheless, the egg incubation period was similar (7–9 days) in the IM and WM samples. Consequently, IM and WM large females of CW ≥ 100 mm are suitable for the larvae production of <em>S. olivacea</em> in captivity.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"42 ","pages":"Article 102718"},"PeriodicalIF":3.2,"publicationDate":"2025-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143578469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological performance and cardiac morphology of Atlantic salmon reared under slow and fast growth conditions","authors":"Malthe Hvas ,&nbsp;Ole Folkedal ,&nbsp;Marco A. Vindas ,&nbsp;Ida B. Johansen","doi":"10.1016/j.aqrep.2025.102730","DOIUrl":"10.1016/j.aqrep.2025.102730","url":null,"abstract":"<div><div>Early rearing environment affects performance later in life. In Atlantic salmon (<em>Salmo salar</em>) aquaculture intensive smolt production has been linked to deviating cardiac morphology and increased mortality risks following stressful events during the marine production phase. To investigate the effects of early growth environment on later life-stages, two smolt groups were produced; a fast-growing group reared at 13 °C under continuous light and a slow-growing group reared at 6 °C under a natural photoperiod. The two groups were smoltified and transferred to 9 °C seawater at the same time and at similar sizes, although the slow smolts were ≈ 1000 day degrees older. Respirometry and swim tunnel experiments were performed to assess physiological performances along with morphological analyses of the hearts. We hypothesized that the slower growth trajectory would allow for the development of heart morphology more resembling that of wild salmon and that this should translate into improved physiological performance. Fast-growing smolt had more misaligned and enlarged bulbi as well as asymmetric ventricles compared to slow-growing smolt. However, contrary to our hypothesis, we did not find clear evidence for impaired physiological performance in fast-growing fish. That is, neither standard nor maximum metabolic rates, absolute critical swimming speed, stress recovery, or haematological parameters at fatigue differed between treatments. Mortality risks associated with deviating cardiac morphology first tend to occur in larger sized fish than investigated here. We therefore conclude that while early rearing environment clearly modulates cardiac morphology, recently seawater adapted Atlantic salmon do not yet show signs of compromised functionality associated with cardiac morphological differences at the whole-animal level. Future research should aim to incorporate larger sized fish in physiological experiments for a more appropriate representation of the latter production phase in Atlantic salmon aquaculture and its associated fish welfare problems.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"42 ","pages":"Article 102730"},"PeriodicalIF":3.2,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143578471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nutritional and functional benefits of Gracilaria tenuistipitata in aquafeed: Impacts on growth, hemato-biochemical profile, and economic efficiency in Nile tilapia culture","authors":"Md. Rajib Sharker ,&nbsp;Al Azim ,&nbsp;Muhammad A.B. Siddik ,&nbsp;Sang Duk Choi ,&nbsp;Palash Halder ,&nbsp;Md. Nazmul Haque ,&nbsp;Md. Ariful Alam","doi":"10.1016/j.aqrep.2025.102734","DOIUrl":"10.1016/j.aqrep.2025.102734","url":null,"abstract":"<div><div>Macroalgae are a valuable source of protein and bioactive compounds like antioxidants, are gaining attention for their nutritional and functional benefits in aquafeed. The study was undertaken to evaluate the efficacy of red algae, <em>Gracilaria tenuistipitata</em> (GT) as aquafeed in Nile tilapia fish culture<em>.</em> Fish were fed for 56 days with 2.5 % inclusion of <em>G. tenuistipitata</em> in diet demonstrated notable difference in growth attributes, and feed utilization parameters compared to the control and other treatment groups. Fish fed a diet with 2.5 % GT inclusion showed a significant increase in erythrocyte count, hemoglobin concentration, packed cell volume, and red blood cell indices compared to the control group. In contrast, these parameters significantly decreased (p &lt; 0.05) at the 5 % inclusion level. The regression analysis indicated that an optimal inclusion level of GT in the diet, ranging from 2.52 % to 2.74 %, is recommended based on improvements in weight gain, specific growth rate, and hematological parameters. The results obtained on histology showed no significant alteration (p &gt; 0.05) of liver architecture in 2.5 % GT diet supplementation. PCA results revealed that supplementing with 2.5 % GT positively influenced both nutritional and hemato-biochemical parameters. Among the treatment groups, 2.5 % GT supplementation demonstrated positive economic efficiency by reducing production costs, achieving a high net return, and enhanced eco-friendly ecosystem. Finally, incorporating 2.5 % <em>G. tenuistipitata</em> in Nile tilapia diets is the most recommendable in terms of growth, physiological performance, and economic profit index.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"42 ","pages":"Article 102734"},"PeriodicalIF":3.2,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143578470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of dietary curcumin supplementation on growth performance, antioxidant capacity, and gene transcriptome in the juvenile Manila clam (Ruditapes philippinarum)","authors":"Yang Zhang ,&nbsp;Hailong Huang ,&nbsp;Kai Liao ,&nbsp;Yuxiang Zhu ,&nbsp;Yang Liu ,&nbsp;Deshui Chen ,&nbsp;Bin Ma ,&nbsp;Hui Ge ,&nbsp;Jilin Xu","doi":"10.1016/j.aqrep.2025.102727","DOIUrl":"10.1016/j.aqrep.2025.102727","url":null,"abstract":"<div><div>The complex conditions in aquaculture environments often lead to oxidative stress in juvenile bivalves, highlighting the need for effective mitigation strategies. This study evaluated the effects of dietary curcumin supplementation on growth performance, antioxidant capacity, and gene transcriptome in the juvenile clam (<em>Ruditapes philippinarum</em>). A total of 36 g of clams (individual body weight: 0.5 ± 0.17 mg, shell length: 1.15 ± 0.15 mm) were equally distributed into six groups (three replicates per group) and fed six microencapsulated diets containing 0, 50, 100, 200, 400, and 800 mg/kg of curcumin for three weeks. Results indicated that curcumin supplementation did not significantly influence growth performance. However, clams in the 200 mg/kg and 400 mg/kg groups exhibited markedly higher activities of catalase, superoxide dismutase (SOD), and glutathione, along with reduced malondialdehyde (MDA) levels compared to the 0 mg/kg group. Following treatment with hydrogen peroxide, the survival rate of clams in 400 mg/kg group was significantly higher than 0 mg/kg group. Meanwhile, the activities of SOD and Glutathione Peroxidase were significantly increased in the 400 mg/kg group, with a corresponding reduction in MDA levels compared to the 0 mg/kg group. The transcriptome analysis further revealed that supplementation with 400 mg/kg curcumin significantly upregulated the expression of genes related to autophagy and lysosomal pathways when compared to the 0 mg/kg group. In conclusion, our study suggested that supplementation with 400 mg/kg curcumin significantly enhanced the antioxidant capacity of clams, providing essential insights for mitigating oxidative stress in juvenile bivalve aquaculture.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"42 ","pages":"Article 102727"},"PeriodicalIF":3.2,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143550834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of hydroxyproline-taurine-cholesterol supplementation on the liver health and lipid metabolisms of turbot Scophthalmus maximus fed Chlorella vulgaris","authors":"Daisong Ma ,&nbsp;Nan Bai ,&nbsp;Shihui Pan ,&nbsp;Chuwen Chen ,&nbsp;Min Gu","doi":"10.1016/j.aqrep.2025.102726","DOIUrl":"10.1016/j.aqrep.2025.102726","url":null,"abstract":"<div><div>Microalgae, such as <em>Chlorella vulgaris</em> (CV), possess significant potential as a protein source for aquafeed. However, it has been demonstrated that CV can cause liver damage in carnivorous fish. The present study was aimed at evaluating a combination of hydroxyproline, taurine, and cholesterol (HTC, in a ratio of 1:1:2) for the purpose of alleviating this issue. Seven experimental diets were formulated. The control diet utilized fishmeal as the exclusive protein source. Three CV diets were designed, in which CV substituted 45 %, 60 %, or 75 % of the fishmeal protein. Moreover, three HTC diets were prepared by incorporating 2 % of HTC into the CV diets. Each of the seven diets was fed to three groups of turbot with an initial average weight of 211.6 ± 1.0 g. The results showed that CV at any assayed level reduced the growth performance and feed intake of turbot and induced inflammatory damage to the liver. CV also disrupted lipid metabolism, as indicated by the significantly lower levels of total triglyceride and total cholesterol in the plasma and bile acids in the liver (<em>P</em> &lt; 0.05). The expression of <em>cyp7α1</em>, which encodes the rate-limiting enzyme for bile acid synthesis, was significantly reduced by CV (<em>P</em> &lt; 0.05). Concurrent supplementation of HTC with CV completely restored bile acid metabolism. The bile acid levels and the expression of <em>cyp7α1</em> in all HTC groups were at the same level as those in the control group (<em>P</em> &gt; 0.05). HTC also increased the total triglyceride and total cholesterol levels in the plasma while reducing the liver lipid content. Moreover, HTC effectively reduced liver injury by alleviating the inflammatory condition and down -regulating the expression of inflammatory-related cytokines induced by CV. In conclusion, the present study provides evidence that the combination of hydroxyproline, taurine, and cholesterol has a hepatic protection function, possibly due to its ability to restore the bile acid status disturbed by CV.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"42 ","pages":"Article 102726"},"PeriodicalIF":3.2,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143550833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic analysis of the toxic effects and potential mechanisms of fenvalerate on the liver of Odontobutis potamophila","authors":"Guoxing Liu ,&nbsp;Tian Zhu ,&nbsp;Zihan Zhou ,&nbsp;You Zheng ,&nbsp;Yanhua Zhao ,&nbsp;Chen Hao ,&nbsp;Shuyan Ding ,&nbsp;Xiuhui Tan ,&nbsp;Qichen Jiang","doi":"10.1016/j.aqrep.2025.102721","DOIUrl":"10.1016/j.aqrep.2025.102721","url":null,"abstract":"<div><div>This study explored the toxic effects of short-term exposure (96 h) to fenvalerate (FEN) at environmental concentrations (C0.5: 0.5 µg/L) and application concentrations (C2: 2 µg/L) on the liver of <em>Odontobutis potamophila</em> and analyzed the potential toxicity mechanisms. The results showed that FEN exposure caused tissue damage and oxidative stress in <em>O. potamophila</em> liver in a concentration-dependent manner. Transcriptomic analysis revealed 2083 differentially expressed genes (DEGs) between control (CK) and C0.5 treatments and 2595 DEGs between CK and C2 treatments. Kyoto Encyclopedia of Genes and Genomes enrichment analysis revealed significant DEG enrichment in pathways such as serotonergic synapse, estrogen signaling, retrograde endocannabinoid signaling, etc. in C0.5, and in pathways such as oxidative phosphorylation, glutathione metabolism, retrograde endocannabinoid signaling, etc. in C2. Gene Ontology enrichment analysis showed significant DEGs enrichment in pathways such as DNA integration, obsolete oxidation-reduction process, regulation of DNA-templated transcription, etc. in C0.5, and in pathways such as obsolete oxidation-reduction process, translation, structural constituent of ribosome, etc. in C2. Investigation of genes related to liver mitochondrial function, including expression of cytochrome b (<em>CYTB</em>), cytochrome c oxidase subunit II (<em>COX2</em>), cytochrome c oxidase subunit III (<em>COX3</em>), and NADH dehydrogenase subunit 1 (<em>ND1</em>), demonstrated that <em>CYTB</em> and <em>ND1</em> were significantly downregulated (<em>P</em> &lt; 0.05) in a concentration-dependent manner, while <em>COX2</em> and <em>COX3</em> were significantly upregulated (<em>P</em> &lt; 0.05) in C0.5 and C2, indicating that FEN could damage mitochondrial function in <em>O. potamophila</em> liver. In summary, this study revealed that the toxic effects of FEN on the liver of O<em>. potamophila</em> manifested as causing oxidative stress, resulting in tissue damage and mitochondrial dysfunction, and its key pathway of action is retrograde endocannabinoid signaling pathway. This results provide a theoretical basis for research on the toxic effects of FEN on aquatic organisms, and assessed the environmental risks of FEN exposure.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"42 ","pages":"Article 102721"},"PeriodicalIF":3.2,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143534576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}