Aquaculture Reports最新文献

{"title":"Effect of temperature stress on the growth, ovary development, antioxidant capacity, sex hormone content, and reproductive-related gene expression of female red swamp crayfish (Procambarus clarkii)","authors":"Yu Su, Pei-Hua Zheng, Li-Xuan Wang, Yao-Peng Lu, Ze-Long Zhang, Xiu-Xia Zhang, Ming-Tao Lin, Jian-An Xian, Dong-Mei Wang, Jun-Tao Li","doi":"10.1016/j.aqrep.2025.103072","DOIUrl":"10.1016/j.aqrep.2025.103072","url":null,"abstract":"<div><div>Red swamp crayfish (<em>Procambarus clarkii</em>) is the most extensively farmed freshwater shrimp species in China. However, the underdeveloped factory breeding technology of <em>P. clarkii</em> has significantly constrained the development of this industry. Temperature changes directly affect the metabolism of <em>P. clarkii</em>, thereby influencing its growth and reproduction. The study aimed to investigate the effects of temperature on growth, ovarian development, antioxidant capacity, sex hormone levels, and reproductive gene expression of female <em>P. clarkii</em>, and to investigate the molecular mechanism through which temperature regulation promotes ovarian development. A total of 250 female <em>P. clarkii</em> (average weight of 13.30 ± 0.31 g) were randomly selected and divided into five groups, with five replicates in each group. Temperature stress experiments were conducted at natural temperature (control group), 17℃, 19℃, 21℃, 25℃, and 29℃. Compared with the control group, the maturity and vitellogenin content of female crayfish at 25℃ were significantly improved, and their growth rate was the highest. Compared with the natural temperature group, levels of glutathione peroxidase, superoxide dismutase, and total antioxidant capacity in the hepatopancreas and hemolymph of the 17℃, 21℃, and 29℃ groups were significantly reduced, while the content of malondialdehyde was significantly increased (<em>P</em> < 0.05), while there was no significant difference in the 25℃ group. At 25℃, the levels of estrogen and progesterone in the hemolymph of female <em>P. clarkii</em> were significantly increased, and the expression levels of vitellogenin receptor (<em>vgr</em>), forkheadboxL2 (<em>foxL2</em>), vitellogenin (<em>vg</em>), proliferating cell nuclear antigen (pcna), and <em>cyclin B</em> in hepatopancreas were significantly upregulated (<em>P</em> < 0.05). At 25°C, estrogen simultaneously activated the expression of <em>vg</em> and <em>vgr</em> genes in the hepatopancreas and ovaries, promoting the efficient synthesis and transport of vitellogenin. Additionally, estradiol enhanced the transcription of foxL2 by binding to the binding protein, maintained ovarian differentiation, induced pcna-mediated proliferation of reproductive cells, and activated <em>cyclin B</em> and cell division cycle 2 (<em>cdc2</em>) genes to promote the meiotic division process of oocytes. These findings demonstrate a core mechanism of reproductive enhancement involving gene activation, cell cycle synergy, and oxidative balance, thereby significantly improving the efficiency of ovarian maturation. In conclusion, the 25℃ is the most effective temperature for promoting the growth and ovarian development of female <em>P. clarkii</em>, and its antioxidant capacity remains at a relatively high level at this temperature, which is conducive to reproductive activities. This study provides a theoretical basis for optimizing the temperature control strategy ","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"44 ","pages":"Article 103072"},"PeriodicalIF":3.7,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144902980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantifying oyster aquaculture lease acceptance: A case study on repurposed Maine lobster pounds","authors":"Ruby Krasnow,&nbsp;Thomas Kiffney,&nbsp;Struan Coleman,&nbsp;Robert Cuddy,&nbsp;Damian C. Brady","doi":"10.1016/j.aqrep.2025.103067","DOIUrl":"10.1016/j.aqrep.2025.103067","url":null,"abstract":"<div><div>Oyster aquaculture holds tremendous potential to diversify coastal economies and increase resilience to climate change, but expansion can conflict with other marine resource users. These conflicts are difficult to characterize quantitatively. Here, we developed an analytical framework using difficulty scores to analyze the difficulty of obtaining an oyster aquaculture lease through publicly available application decisions in Maine, USA. We applied this quantitative framework to a case study examining the potential of using existing fishery infrastructure as a means of diversifying working waterfronts. Specifically, repurposing tidal impoundments historically used to store American lobsters (lobster pounds) for oyster aquaculture has been proposed as an actionable method of overcoming obstacles to aquaculture development in Maine. While the environmental suitability of lobster pounds for oyster aquaculture has been established, there has been minimal exploration of potential regulatory or socioeconomic benefits. We assessed the relative difficulty of obtaining a lease for an aquaculture site within a lobster pound compared to a traditional open-water site. Our analysis demonstrated that the enclosed and privately-owned nature of a lobster pound eliminated many issues that commonly arise during oyster farm site selection, including conflict with riparian landowners, impeded navigation, and interference with commercial fishing or other existing water uses. These results provide the first empirical evidence that repurposing vacant waterfront infrastructure like lobster pounds can minimize social, institutional, financial, and logistical barriers to marine aquaculture development.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"44 ","pages":"Article 103067"},"PeriodicalIF":3.7,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144895516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The evaluation of asparagus (Asparagus officinalis L.) root powder as a feed supplement on enhancing growth, immunity, and alleviating acute crowding stress in rainbow trout (Oncorhynchus mykiss)","authors":"Mahyar Zare ,&nbsp;Ghasem Rashidian ,&nbsp;Hung Quang Tran ,&nbsp;Thora Lieke ,&nbsp;Tatyana Gebauer ,&nbsp;Markéta Dvořáková Prokešová ,&nbsp;Deepali Rahi Roy ,&nbsp;Dalibor Kodrík ,&nbsp;Helena Štěrbová ,&nbsp;Vlastimil Stejskal","doi":"10.1016/j.aqrep.2025.103060","DOIUrl":"10.1016/j.aqrep.2025.103060","url":null,"abstract":"<div><div>In this study, Asparagus (<em>Asparagus officinalis</em> L.) root powder was evaluated as a feed supplement at levels 10 (A10), 20 (A20), 30 g/kg (A30), and control (without supplement) for rainbow trout (<em>Oncorhynchus mykiss</em>) with initial body weight (20.30 ± 0.17 g). Based on our findings, growth performance remained unchanged across all groups (<em>p</em> &gt; 0.05). Red blood cells were found significantly higher in the asparagus-fed groups compared to the control (<em>p</em> &lt; 0.05). Hematocrit and hemoglobin levels were significantly increased in the A30 and A10 groups, respectively (<em>p</em> &lt; 0.05). Serum cholesterol and triglyceride levels were significantly lower in A20 compared to the control (<em>p</em> &lt; 0.05). Lymphoid cell phagocytic activity was significantly higher in control compared to the A10 and A20. However, myeloid cell phagocytic activity in A10 and A20 was significantly higher than control (<em>p</em> &lt; 0.05). Before stress, serum cortisol levels were similar across all groups (<em>p</em> &gt; 0.05); however, the A30 group exhibited the lowest glucose levels (<em>p</em> &lt; 0.05). One hour post-stress, cortisol levels were significantly higher in A10 and A30 (<em>p</em> &lt; 0.05), while glucose levels remained unchanged (<em>p</em> &gt; 0.05). A30 showed the lowest level of cortisol at 24 h post-stress (<em>p</em> &lt; 0.05) despite no significant changes in glucose among all other groups (<em>p</em> &gt; 0.05). In conclusion, asparagus root powder at the tested levels did not improve fish growth, although the positive impacts on hematological parameters, serum biochemistry, immune responses, and post-stress cortisol regulation in rainbow trout merit further investigation.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"44 ","pages":"Article 103060"},"PeriodicalIF":3.7,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144895515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing the effects of Rheum ribes extract on growth, immunity, antioxidant response, and gene expression in triploid rainbow trout (Viviers Strain) under river conditions","authors":"Majid Khanzadeh ,&nbsp;Hamidreza Tabibi ,&nbsp;Seyed Hossein Hoseinifar ,&nbsp;Mohammad Rahmani ,&nbsp;Hamed Azadi ,&nbsp;Amir Mohammad Khajavi ,&nbsp;Hien Van Doan","doi":"10.1016/j.aqrep.2025.103056","DOIUrl":"10.1016/j.aqrep.2025.103056","url":null,"abstract":"<div><div>This research aimed to examine the impact of <em>Rheum ribes</em> (RRE) on growth performance, immune function, antioxidant activity, and gene expression in triploid rainbow trout reared in river conditions. For eight weeks, the fish were given diets that included different levels of RRE extract, ranging from no supplement at all to incremental additions of 0.5 %, 1 %, 1.5 %, 2 %, 2.5 %, and up to 3 %. Growth criteria such as weight gain, final weight (RRE 1–3 %), specific growth rate, and weight gain percentage (RRE 2 %) were significantly elevated, and the feed conversion ratio in the RRE2 % was significantly decreased against the control (Ctrl) group (<em>P</em> &lt; 0.05). Hematological parameters, except for white blood cells (RRE 1.5 and 2 %), were not significant compared to the Ctrl group (<em>P</em> &gt; 0.05). Serum biochemical parameters were not significantly different at any RRE doses compared to the Ctrl group (<em>P</em> &gt; 0.05). Unlike other biochemical parameters, cortisol and glucose were significantly increased in all groups receiving RRE, except the group receiving 0.5 % RRE, compared to the control group (<em>P</em> &lt; 0.05). Serum catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) levels significantly increased at RRE doses 1–3, while serum malondialdehyde significantly decreased in these groups compared to the Ctrl (<em>P</em> &lt; 0.05). Immune parameters such as total immunoglobulin (RRE 1–2 %), lysozyme (RRE 0.5–2 % and 3 %), complement 3 (RRE 2–3 %), protease (RRE 0.5 %, 2 %, and 3 %), nitroblue tetrazolium (all RRE levels), and serum bactericidal activity against <em>Streptococcus iniae</em> (RRE 2 %) were significantly increased versus the Ctrl group (<em>P</em> &lt; 0.05). Digestive enzymes such as trypsin (RRE 1.5–3 %), alpha-amylase (RRE 1–3 %), and lipase (RRE 1.5–2.5 %) showed a notable elevate against the Ctrl group (<em>P</em> &lt; 0.05). The expression of antioxidant genes, including Nuclear factor erythroid 2-related factor 2 (Nrf2), CAT, SOD, GPx, and glutathione reductase, was upregulated at all RRE levels against the Ctrl group (<em>P</em> &lt; 0.05). Other genes, such as tumor necrosis factor-alpha (TNF-α) and insulin-like growth factor 1 (IGF-1), were upregulated at RRE levels of 1.5–3 %, while the interleukin-8 gene was upregulated at all RRE levels, and interleukin-1 beta was upregulated at RRE levels of 1 %, 2 %, and 3 % in relation to the Ctrl group (P &lt; 0.05). In general, it can be concluded that the 2 % dose of RRE is the optimal level, showing a significantly greater effect on growth, immune response, antioxidant activity, digestive enzymes, and immune-antioxidant genes expression in rainbow trout relative to the other doses.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"44 ","pages":"Article 103056"},"PeriodicalIF":3.7,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144890500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Suitable formulation of moist diet for newly hatched bigfin reef squid (Sepioteuthis lessoniana)","authors":"Natthawut Chanlek ,&nbsp;Jirapan Satjarak ,&nbsp;Yuzuru Ikeda ,&nbsp;Nutt Nuntapong ,&nbsp;Karun Thongprajukaew","doi":"10.1016/j.aqrep.2025.103061","DOIUrl":"10.1016/j.aqrep.2025.103061","url":null,"abstract":"<div><div>The bigfin reef squid (<em>Sepioteuthis lessoniana</em>) is a cephalopod with significant potential as a farmed species. However, uncertainty surrounds the optimal feed formulation and feed strategy for <em>S. lessoniana</em>, especially for the early stages. This study aimed to develop a suitable moist diet formulation to improve growth performance and feed utilization of young squid. Initially, eight moist meat-based diet formulations were screened using an <em>in vitro</em> digestibility technique. Four formulations were considered suitable: the meat of Indian mackerel (IM), the conventional ration of whiteleg shrimp (WS), Indian mackerel and trash fish (IT), and Indian mackerel, striped catfish and trash fish (IST). An <em>in vivo</em> feeding trial was conducted, using a completely randomized design with triplicate groups of eight-day-old squid (39.0 ± 0.1 mg initial weight) for two weeks. Newly hatched squid fed the IST diet showed significantly higher growth than other groups, followed by squid fed the IT diet (<em>P</em> &lt; 0.05). The IST group showed the lowest feed conversion ratio but moderate protein efficiency ratio. Feed intake and survival rate, however, did not vary significantly across the groups. Proteinase-specific activity was higher in the IST group, and levels of mantle myosin and its sum with actin were significantly elevated compared to other groups. There were no significant differences in mantle RNA concentration, protein concentration, and RNA/protein ratio between IT and IST groups and the WS group. These findings suggest that a well-formulated artificial diet for newly hatched bigfin reef squid can improve their growth and feed efficiency.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"44 ","pages":"Article 103061"},"PeriodicalIF":3.7,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144892245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The improved growth performance of Cyprinus carpio by dietary yeast culture depends on improvement of intestinal structure and digestive enzyme activities rather than changes of intestinal microbiota composition","authors":"Shengqiang Tao ,&nbsp;Jiahao Wang ,&nbsp;Zhiyuan Xue ,&nbsp;Yichen Bai ,&nbsp;Yi Wang ,&nbsp;Wenhao Zhou ,&nbsp;Tingyin Liang ,&nbsp;Yuhong Yang","doi":"10.1016/j.aqrep.2025.103053","DOIUrl":"10.1016/j.aqrep.2025.103053","url":null,"abstract":"<div><div>The ban of antibiotic growth promoters can influence the total production of aquaculture. Yeast culture (YC) possesses the ability to enhance immunity and has been supplemented into diet to try to increase production. To investigate the effects of YC on the growth and its mechanisms, four diets supplemented with 0 g/kg (Y0), 10 g/kg (Y1), 20 g/kg (Y2), 30 g/kg (Y3) YC were assigned to <em>Cyprinus carpio</em> for 8 weeks (three replicates per diet, 30 fish in each replicate). The results showed that dietary 10 g/kg YC significantly increased weight gain rate (WGR), protein efficiency ratio (PER), specific growth rate (SGR) and reduced feed conversion ratio (FCR) (<em>P</em> &lt; 0.05). Also, the apparent digestibility of lipids and proteins was significantly increased by dietary 10 g/kg YC compared with the Y0 group (<em>P</em> &lt; 0.05). Meanwhile, fish fed 10 g/kg YC showed significantly higher intestinal villus height and width as well as the digestive enzyme activities of trypsin and lipase than Y0 group (<em>P</em> &lt; 0.05). However, no significant differences was found in the composition of intestinal flora between the Y1 and Y0 groups (<em>P</em> &gt; 0.05). Whereas, excessive levels of YC significantly reduced abundances of Fusobacteriota and increased that of Proteobacteria (<em>P</em> &lt; 0<em>.</em>05). Simultaneously, the growth performance, apparent digestibility of dry matter and protein and intestinal trypsin activity in the Y3 group were also significantly lower than that of Y1 group (<em>P</em> &lt; 0.05). In summary, our results supported the applicability of dietary 10 g/kg YC as a growth promoter for common carp through the improvement of intestinal digestive enzymes and intestinal structure but not gut microbial composition, and suggested that excessive levels of YC may cause imbalances in the gut flora, resulting in growth inhibition.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"44 ","pages":"Article 103053"},"PeriodicalIF":3.7,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144890501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simultaneous reduction of dietary protein and daily feeding rate in biofloc system: Effect on water quality, growth, digestive and antioxidant capacity, immune response, and resistance to Aeromonas hydrophila in common carp","authors":"Hossein Adineh ,&nbsp;Mahdi Naderi ,&nbsp;Mohammad Amini Chermahini ,&nbsp;Golbahar Zeitounli ,&nbsp;Mohammad Meysam Salahi Ardekani","doi":"10.1016/j.aqrep.2025.103052","DOIUrl":"10.1016/j.aqrep.2025.103052","url":null,"abstract":"<div><div>This experiment evaluated the impacts of simultaneous reduction of dietary protein and daily feeding rate in a biofloc system on performance of common carp, <em>Cyprinus carpio</em>. Juveniles (19.70 ± 1.55 g) were randomly placed in 15 tanks (200 L, 50 fish per tank) and divided into five groups including fish fed diets containing 35 % protein at 3 % (P35–3 %) or 2 % (P35–2 %) body weight (BW) per day and diets containing 25 % protein at 3 % (P25–3 %) or 2 % (P25–2 %) BW per day in biofloc culture system, and control group (clear water system, 35 % dietary protein, 3 % BW). Each group had three tanks. After 60 days, fish were exposed to <em>Aeromonas hydrophila</em> and the relative percentage survival (RPS) was determined. The lowest level of total ammonia was obtained in P25–2 % tanks. Higher growth of fish was obtained in P35–2 %, but it was not significantly different from the P25–2 % (P &gt; 0.05). P35–2 % and P25–2 % groups had higher amylase and lipase activities compared to P25–3 % and control (P &lt; 0.05). Higher antioxidant enzyme (CAT, SOD, and GPx) activities and lower liver malondialdehyde were obtained in P35–2 %, P25–2 %, and P25–3 % groups compared to P35–3 % and control (P &lt; 0.05). Serum alternative complement (ACH50) activity was highest and cortisol level was lowest in P35–2 % and P25–2 % groups. Serum lysozyme activity was significantly higher in P35–2 % group than the P35–3 % and control (P &lt; 0.05). Serum total immunoglobulin content was highest in P25–2 %. At 5 days after challenge, the RPS was highest in P35–2 % and P25–2 % and lowest in control, which can be described by using more bioflocs at a lower feeding rate. Overall, it is recommended to the aquaculture industry to reduce dietary protein from 35 % to 25 % and daily feeding rate from 3 % to 2 % BW simultaneously in biofloc system for common carp juveniles.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"44 ","pages":"Article 103052"},"PeriodicalIF":3.7,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144890579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of WSSV infection on immunity, intestinal microbial composition and its derived metabolites synthesis in Procambarus clarkii","authors":"Xiaochuan Zheng ,&nbsp;Jie Yang ,&nbsp;Feifan Xu ,&nbsp;Qunlan Zhou ,&nbsp;Cunxin Sun ,&nbsp;Aimin Wang ,&nbsp;Jinjuan Wan ,&nbsp;Jiajia Li ,&nbsp;Bo liu","doi":"10.1016/j.aqrep.2025.103055","DOIUrl":"10.1016/j.aqrep.2025.103055","url":null,"abstract":"<div><div>Intestinal microbes play a pivotal role in the pathogenesis of disease, with derived metabolites acting as key mediators. The WSSV results in considerable economic losses to the crayfish aquaculture industry. We constructed an artificial infection model of WSSV in crayfish and conducted a comprehensive investigation into the alterations in non-specific immunity, intestinal microbial structure, and functional metabolites of crayfish following WSSV infection by integrating 16S rDNA and targeted metabolomics techniques. WSSV infection induced oxidative stress and hepatopancreatic injury. RT-PCR results indicated that WSSV infection resulted in a significant upregulation of the transcript levels of genes related to the proPO system (<em>proPO</em> and <em>Crustin</em>), the inflammatory response (<em>Relish</em>, <em>Cox-1</em> and <em>Cox-2</em>) and autophagy (<em>LC3</em> and <em>Atg2</em>) in crayfish hemocytes. Transcript levels of genes associated with apoptosis (<em>Caspase3</em>), lectins (<em>L-Lectin</em>) and phagocytosis (<em>Rab5</em>) were significantly reduced. The abundance of the Proteobacteria and the <em>Aeromonas</em> genus markedly increased, while the <em>Anaerorhabdus furcosa</em> and <em>Akkermansia</em> was significantly diminished in infected crayfish. BugBase prediction indicated the pathogenic potential and oxidative stress tolerance of the intestinal microbiota increased following infection. Targeted metabolomic analyses revealed a significant decrease in acetate and a significant increase in GCDCA in the chyme of the infected crayfish. The results suggest that intestinal microorganisms and their specific functional metabolites, acetate and GCDCA, are involved in WSSV infection and immune mobilisation of crayfish. The present study offers new insights and avenues for understanding the pathogenic mechanisms of WSSV and precise targets for the development of novel anti-WSSV modulators.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"44 ","pages":"Article 103055"},"PeriodicalIF":3.7,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144890576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Substituting photoautotrophic with heterotrophic Chlorella species for water quality regulation and microbial community modification in Litopenaeus vannamei pond culture","authors":"Chu-er Song ,&nbsp;Zhen Meng ,&nbsp;Bangyin Zhang ,&nbsp;Lin Yang ,&nbsp;Xiaoxuan Sun ,&nbsp;Zheng Zhang ,&nbsp;Xiaojin Song","doi":"10.1016/j.aqrep.2025.103049","DOIUrl":"10.1016/j.aqrep.2025.103049","url":null,"abstract":"<div><div>The study aimed to assess the replacement of photoautotrophic cultivated <em>Chlorella vulgaris</em> with heterotrophic cultivated <em>C. pyrenoidosa</em> as the inoculated algal fluid for analyzing effluent nutrient removal and regulating bacterial community dynamics in <em>Litopenaeus vannamei</em> outdoor pond culture system. Two separate treatment outdoor earthen ponds were utilized; one supplemented with <em>C. vulgaris</em> (CV pond) while the other supplemented with <em>C. pyrenoidosa</em> (CP pond), each stocked at a density of 80 animals m⁻³ in the two ponds for a period of 30 days. Regular sampling was performed on water and <em>L. vannamei</em> individuals from both sets of ponds to evaluate water nutrient parameters, bacterial community composition, and growth parameters correspondingly. The shrimp in the CP pond exhibited a weight gain rate and specific growth rate of approximately 157.32 ± 0.43 % and 3.11 ± 0.01 %, respectively, while those in the CV pond demonstrated rates around 159.53 ± 0.36 % and 3.15 ± 0.01 %, with no statistically significant differences observed between the two groups. Consequently, both ponds displayed similar patterns of microalgae proliferation after inoculation throughout the 30-day experiment, with no significant variation detected in algal cell density. The inoculation with <em>C. pyrenoidosa</em> demonstrated enhanced removal efficiency of PO₄^3--P and NH₄⁺-N, along with regulation of pH and BOD/COD levels in shrimp ponds. Inoculating with <em>C. pyrenoidosa</em> further enhances microbial diversity and richness within aquaculture ponds by promoting an abundance of beneficial bacterial taxa such as Proteobacteria, Actinobacteria, Alphaproteobacteria, Gracilibacteria, Luteolibacter, and Exiguobacterium. Redundancy analysis reveals that microalgae species primarily regulate bacterial communities by exerting influence on water quality factors. These findings offer crucial insights into the utilization of heterotrophic cultivation microalgae for shrimp aquaculture by examining their impact on water quality factors and microbial community structures within shrimp cultural ecosystems.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"44 ","pages":"Article 103049"},"PeriodicalIF":3.7,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144890577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nutrient dynamics, environmental impacts, and feed efficiency in intensive whiteleg shrimp (Litopenaeus vannamei) farming on sandy soils in Ninh Thuan, Vietnam","authors":"Thu Thuy Cao ,&nbsp;Hung Anh Le ,&nbsp;Gauthier Eppe","doi":"10.1016/j.aqrep.2025.103050","DOIUrl":"10.1016/j.aqrep.2025.103050","url":null,"abstract":"<div><div>Whiteleg shrimp (<em>Litopenaeus vannamei</em>) farming has expanded rapidly on sandy coastal soils in Vietnam’s South Central region. These permeable soils, combined with high feed input and limited waste treatment, raise concerns about nutrient pollution and environmental degradation. This study assessed nutrient dynamics and environmental impacts in a typical intensive shrimp pond system in Ninh Thuan province over a 66-day culture cycle. Physical, chemical, and biological parameters were monitored, including temperature, salinity, total suspended solids, chemical and biological oxygen demand, total nitrogen, total phosphorus, ammonium, microalgae composition, and bacterial counts. A nutrient mass balance showed that commercial feed contributed 81.9 % of total nitrogen and 87.8 % of total phosphorus inputs. However, nutrient utilization was low: only 13.8 % of nitrogen and 11.4 % of phosphorus were retained in shrimp biomass. The remaining nutrients were lost through multiple pathways: 23.2 % of nitrogen and 1.8 % of phosphorus in effluent, 10.3 % of nitrogen and 2.5 % of phosphorus in pond sludge, and over 50 % of nitrogen and 84 % of phosphorus unaccounted for due to volatilization, denitrification, and sedimentation. 1000 kg of shrimp harvested discharged approximately 263.6 kg of nitrogen, 5.02 kg of phosphorus, and 12,175 liters of nutrient-rich wastewater. Although sludge represented only 5 % of total discharged water, it carried a disproportionately high nutrient load, posing risks to soil, groundwater, and nearby coastal ecosystems. These results highlight critical inefficiencies in nutrient use and significant environmental risks. Effective strategies, including lower feed conversion ratio, improved feed management, and dedicated sludge and wastewater treatment systems, are essential for sustainable shrimp aquaculture on sandy soils.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"44 ","pages":"Article 103050"},"PeriodicalIF":3.7,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144890580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}