Aquaculture Reports最新文献

{"title":"Comparative proteomic analysis of the three developmental stages and adults of Pacific abalone","authors":"Jeong-hun Mok ,&nbsp;Van-An Duong ,&nbsp;Yejin Jeon ,&nbsp;Jong-Moon Park ,&nbsp;Mi Ae Kim ,&nbsp;Ji-Hye Han ,&nbsp;Young Chang Sohn ,&nbsp;Hookeun Lee","doi":"10.1016/j.aqrep.2025.103300","DOIUrl":"10.1016/j.aqrep.2025.103300","url":null,"abstract":"<div><div>Abalone (<em>Haliotis discus hannai</em>) undergoes developmental transitions involving complex morphological and physiological changes from larvae to adults. To understand the molecular basis of these processes, we employed tandem mass tag-based quantitative proteomics across five developmental stages (trochophore and veliger larvae, juvenile, adult males and females).</div><div>A total of 4956 proteins were identified across all stages. The earliest transition from trochophore to veliger larvae showed 66 differentially expressed proteins, predominantly upregulated structural and motility proteins such as myosin and tektin, reflecting muscle and ciliary development. The notable finding was the juvenile stage, which exhibited the substantial proteomic changes with more than 1000 proteins upregulated. These juveniles showed extensive metabolic pathway activation with ATP-citrate synthase-like isoform X1 markedly upregulated, while adults demonstrated metabolic downregulation and sex-specific reproductive protein upregulation including sperm-associated antigen 6 in males and vitellogenin-3-like in females.</div><div>These findings indicate that the most significant proteome shift occurs at the juvenile stage, marked by a transition from structure- and motility-related proteins predominant in the larval stage to metabolism-related proteins. This shift reflects the culmination of metamorphosis and the onset of subsequent body growth. Subsequently, adults showed a clear proteome transition toward reproduction-related protein expression. These results demonstrate that the juvenile stage serves as a critical transition point between larval and adult stages, thus offering molecular insights for stage-specific aquaculture optimization such as environmental and nutritional strategies.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"46 ","pages":"Article 103300"},"PeriodicalIF":3.7,"publicationDate":"2026-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145787124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of acute cold and heat shocks on the protein profile of crayfish hemolymph: Implications for crustacean adaptation to thermal stress","authors":"Kifayatullah Mengal ,&nbsp;Golara Kor ,&nbsp;Valentina Siino ,&nbsp;Fredrik Levander ,&nbsp;Hamid Niksirat","doi":"10.1016/j.aqrep.2025.103265","DOIUrl":"10.1016/j.aqrep.2025.103265","url":null,"abstract":"<div><div>Temperature, a key environmental stressor, can induce changes at the molecular levels in the body of living beings, which are necessary for adaptation and survival under altered conditions. We investigated the effects of acute cold (3 °C) and heat (32 °C) shocks on hemolymph protein profiles in marbled crayfish. Results showed that cold shock induced a metabolic shift toward glucose production by increasing enzymes for breaking down glycogen and upregulating enzymes related to glycolysis, such as glycogen phosphorylase, glyceraldehyde-3-phosphate dehydrogenase, enolase, and <span>L</span>-lactate dehydrogenase in the hemolymph of crayfish. The upregulation of proteins such as filamin-A, alpha-actinin, and tubulin beta may indicate that immune cells in the hemolymph strengthen their survival during cold stress through reinforcement of the cytoskeletal rigidity. Shifts in the abundance of immunity-related proteins such as masquerade-like and β-1,3-glucan-binding proteins suggest that the immune system of decapods can adapt to thermal stresses via remodeling the extracellular matrix and pattern recognition receptors, ultimately modulating host defense strategies by shifting between phagocytosis and melanization. Furthermore, the regulation of reproduction-associated proteins indicates that thermal shock may affect the capacity for reproduction. These findings offer insight into how decapods cope with thermal stresses and may support strategies to protect them in farmed environments, especially under climate change. Data are available via ProteomeXchange with identifier PXD065043.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"46 ","pages":"Article 103265"},"PeriodicalIF":3.7,"publicationDate":"2026-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145682980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immune and metabolic mechanisms underlying fishmeal replacement with Tenebrio molitor in enhancing Pseudobagrus ussuriensis resistance to Aeromonas veronii","authors":"Yang Cao ,&nbsp;Ruyi Hu ,&nbsp;Chenfei Yang ,&nbsp;Huanhuan Miao ,&nbsp;Jing Gao ,&nbsp;Yu Liu ,&nbsp;Xianghong Dong ,&nbsp;Xianzong Wang ,&nbsp;Qing Liu","doi":"10.1016/j.aqrep.2025.103323","DOIUrl":"10.1016/j.aqrep.2025.103323","url":null,"abstract":"<div><div>Fishmeal, the predominant protein source in aquaculture, is increasingly constrained by resource depletion and price volatility, posing challenges to the sustainability of the industry. <em>Tenebrio molitor</em>, rich in high-quality protein and immunologically active compounds, represents a promising alternative. However, its role in modulating fish immunity and metabolism remains poorly characterized. In this study, juvenile <em>Pseudobagrus ussuriensis</em> were fed diets containing 0 %, 10 %, 20 %, or 30 % <em>T. molitor</em> for 90 days, followed by an <em>Aeromonas veronii</em> challenge. Liver tissues were sampled at 0, 24, and 48 h post-infection for histological, biochemical, and transcriptomic analyses. H&amp;E staining indicated that higher dietary inclusion of <em>T. molitor</em> effectively alleviated liver damage. ELISA results showed increased activities of immune effector enzymes (ACP, ALP, LZM) with infection time and replacement ratio, whereas antioxidant indicators (SOD, CAT, T-AOC) decreased and MDA levels increased, suggesting that immune activation may be accompanied by oxidative stress. Transcriptomic profiling identified 830 and 68 differentially expressed genes in the 30 % group compared to the 0 % group at 24 and 48 h, respectively. Antigen processing and presentation pathways were significantly enriched at both time points. STEM analysis revealed persistent activation of Jak–STAT and AGE–RAGE signaling pathways, along with dynamic changes in lipid and porphyrin metabolism linked to energy homeostasis and inflammatory responses. WGCNA demonstrated that both replacement level and infection time co-regulated gene modules associated with immune and metabolic functions. RT–qPCR validation corroborated the transcriptomic findings. Collectively, these findings indicate that dietary supplementation with <em>T. molitor</em> enhances immune function in <em>P. ussuriensis</em>, underscoring its potential as a sustainable alternative to fishmeal. The coordination between immune and metabolic pathways provides mechanistic insights and practical guidance for developing efficient and environmentally sustainable protein sources in aquaculture.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"46 ","pages":"Article 103323"},"PeriodicalIF":3.7,"publicationDate":"2026-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145972718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of replacing fish meal with stalked sea squirt (Styela clava) tunic waste on growth performance, health status, and skin pigmentation in juvenile red sea bream (Pagrus major)","authors":"Hwa Yong Oh ,&nbsp;Tae Hoon Lee ,&nbsp;Da Ye Kang ,&nbsp;Young Wook Kim ,&nbsp;Do-Hyun Kwon ,&nbsp;Bo Seong Gu ,&nbsp;Chan-Hyeon Ahn ,&nbsp;Hyun-Soo Kim ,&nbsp;Ki Wook Lee ,&nbsp;Jin Choi ,&nbsp;Hee Sung Kim ,&nbsp;Seung Hyeon Kim","doi":"10.1016/j.aqrep.2026.103372","DOIUrl":"10.1016/j.aqrep.2026.103372","url":null,"abstract":"<div><div>This study evaluated the effects of replacing fish meal (FM) with stalked sea squirt (<em>Styela clava</em>) tunic (TSC) on growth performance, health status, and skin pigmentation in juvenile red sea bream (<em>Pagrus major</em>). Five isonitrogenous and isolipidic diets were formulated, with TSC replacing FM at 0 % (TSC0), 5 % (TSC5), 10 % (TSC10), 15 % (TSC15), and 20 % (TSC20). After an 8-week feeding trial, TSC inclusion up to 10 % did not adversely affect growth, whereas TSC15 and TSC20 significantly reduced weight gain and feed efficiency. Immune response indicators, including lysozyme activity, immunoglobulin M, and interleukin-1, exhibited a linear decline with increasing dietary TSC levels (<em>P</em> &lt; 0.05), suggesting potential immunosuppressive effects at higher inclusion rates. Skin color analysis revealed linear increases in redness (a*) and yellowness (b*) with higher dietary TSC levels. TSC15 significantly enhanced cranial skin a* and b* values compared to TSC0, although differences from TSC5, TSC10, and TSC20 were not significant. These results indicate that TSC can replace up to 10 % of FM in juvenile <em>P. major</em> diets without compromising growth or health, while higher replacement levels enhance skin pigmentation but may entail trade-offs in immune function and nutritional performance.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"46 ","pages":"Article 103372"},"PeriodicalIF":3.7,"publicationDate":"2026-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145972971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The characteristics of sheltering behavior in the sea cucumber Apostichopus japonicus","authors":"Xiang Li,&nbsp;Sihan Wang,&nbsp;Qiuzhen Song,&nbsp;Zihe Zhao,&nbsp;Yingzhuo Sun,&nbsp;Jun Ding,&nbsp;Chong Zhao","doi":"10.1016/j.aqrep.2025.103355","DOIUrl":"10.1016/j.aqrep.2025.103355","url":null,"abstract":"<div><div>Based on the sheltering behavior, shelters are widely applied to enhance the aquaculture efficiency of sea cucumbers. However, the characteristics of sheltering behavior are still poorly understood in sea cucumbers. This study investigated the characteristics of the sheltering behavior of the sea cucumber <em>Apostichopus japonicus</em> under individual, group, and interspecific levels. In the individual experiment, the research findings showed that, regardless of whether it was day or night, there were no significant differences in the movement randomness, first-arrival time, and movement speed of sea cucumbers. Regardless of whether it was day or night, the residence time of sea cucumbers in the group with shelters was significantly longer than that in the group without shelters. This indicates that sea cucumbers are random into shelters rather than random leaving the shelter, no matter in the day or at night. In the group experiment and inter-species experiment, no significant differences existed in the residence time, the first-arrival time and the movement speed. This suggests that the basic characteristics of sheltering behavior of sea cucumbers is not affected by the interaction at the present densities. The present study clarified the characteristics of sheltering behavior of sea cucumbers under individual, group, and inter-species interactions. This research uncovered the characteristics of the sheltering behavior of <em>A. japonicus</em> and offered crucial information for enhancing the aquaculture efficiency of sea cucumbers in shelter.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"46 ","pages":"Article 103355"},"PeriodicalIF":3.7,"publicationDate":"2026-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145972969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential utility of dietary formate supplementation in facilitating growth performance and intestinal health for juvenile Songpu mirror carp (Cyprinus carpio Songpu)","authors":"Jiaxin Li ,&nbsp;Ze Fan ,&nbsp;Yan Wang ,&nbsp;Di Wu ,&nbsp;Jinnan Li ,&nbsp;Chenhui Li ,&nbsp;Yongjie Jiang ,&nbsp;Haitao Zhang ,&nbsp;Liansheng Wang","doi":"10.1016/j.aqrep.2025.103335","DOIUrl":"10.1016/j.aqrep.2025.103335","url":null,"abstract":"<div><div>This research was designed to assess the impacts of sodium formate on the growth performance, blood biochemical parameters, and intestinal health of Songpu mirror carp (<em>Cyprinus carpio Songpu</em>). Four isonitrogenous and isolipidic diets supplemented with varying concentrations of sodium formate (0 %, 0.5 %, 1 %, and 1.5 %, corresponding name: C0, FA1, FA2 FA3) were fed with an initial body weight of 6.61 ± 0.02 g for 8 weeks. Our results showed that the weight gain rate and specific growth rate initially trended upward, followed by a decline with increasing sodium formate in diets. The highest value occurred in the FA1 group, significantly exceeding that of the C0 group. By contrast, the FA1 group had a substantially lower feed conversion ratio than the C0 group. Among the serum indicators, the glucose content in the FA1 group was notably greater than that in other groups, while triglyceride levels were significantly reduced in FA1 group. Protease activity in the midgut was significantly enhanced in the FA1 group. In addition, the FA1 group showed a marked increase in the expression of sod, together with nrf2 and cat gene expressions, compared with the control group. In terms of intestinal immunity, 0.5 % sodium formate significantly regulated the expression of the zo-1 gene. The highest abundance of the phylum firmicutes was found in the 0.5 % sodium formate-treated group at the phylum level, while a higher abundance of <em>Lactococcus lactis</em> was found in the 0.5 % sodium formate-treated group at the genus level. In conclusion, 0.5 % sodium formate was identified as the optimal inclusion level in the diet.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"46 ","pages":"Article 103335"},"PeriodicalIF":3.7,"publicationDate":"2026-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145973203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing live transport conditions for Macrobrachium rosenbergii: Temperature and prawn-water mass ratio effects on survival, stress physiology and metabolic adaptation","authors":"Xuenan Li ,&nbsp;Xiaoqi Gu ,&nbsp;Huici Yang ,&nbsp;Simin You ,&nbsp;Nauman Khan ,&nbsp;Xilin Dai","doi":"10.1016/j.aqrep.2025.103240","DOIUrl":"10.1016/j.aqrep.2025.103240","url":null,"abstract":"<div><div>This study aimed to optimize live transport strategies for <em>Macrobrachium rosenbergii</em> by systematically examining transport conditions and underlying metabolic response mechanisms, with the ultimate goal of improving transport survival and product quality. The study analyzed the influence of varying transport temperatures and prawn-to-water mass ratios on survival rate, physiological indexes and tissue integrity in <em>M. rosenbergii</em>. The findings indicated that ammonia nitrogen accumulation emerged as the primary limiting factor for transportation survival. During short-duration transport (12 h), a combination of low temperature (16℃) and a high prawn-to-water mass ratio (1:2) significantly enhanced survival rates (up to 100 % in the T16–3 group) and substantially mitigated immune stress and oxidative damage. However, after extended-duration transport (24 h), the antioxidant system was gradually depleted, lipid peroxidation increased, and significant damage to hepatopancreatic tissue occurred. The survival rate in the high temperature (20℃) and anhydrous transport group declined sharply due to accelerated metabolism and an imbalance in osmotic pressure. Metabolomics techniques were subsequently used to examine the effects of live transport on the metabolic processes of <em>M. rosenbergii</em>. The findings revealed that the T16–3 group positively regulates environmental stresses by providing energy and performing detoxification functions through amino acid metabolic pathways. Groups T16–1 and T16–2 reduced immunity and energy availability by inhibiting autophagy-related and linoleic acid metabolic pathways. Groups T18–3 and T20–3 resisted the stressful environment by activating a wide range of metabolic changes, but still caused severe damage to the prawn. This study offers a scientific foundation for optimizing the live transport of <em>M. rosenbergii</em>, thereby improving transport efficiency, reducing costs, and ensuring product quality and freshness.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"46 ","pages":"Article 103240"},"PeriodicalIF":3.7,"publicationDate":"2026-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145734109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Citric acid could strengthen the intestinal tight junction barrier and improve intestinal immune function in vivo and vitro experiments of hybrid grouper (Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂)","authors":"Jiacheng Zhang ,&nbsp;Fred Bissih ,&nbsp;Beiping Tan ,&nbsp;Shuang Zhang ,&nbsp;Qihui Yang ,&nbsp;Xiaohui Dong ,&nbsp;Shuyan Chi","doi":"10.1016/j.aqrep.2025.103282","DOIUrl":"10.1016/j.aqrep.2025.103282","url":null,"abstract":"<div><div>In this experiment, the primary intestinal epithelial cells (IECs) inflammation model was constructed to investigate the intestinal injury mechanism under high level addition of plant-derived proteins in hybrid grouper (<em>Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂</em>), and citric acid (CA) addition was used to investigate the protective effect in the primary IECs inflammation model. The vitro experiment was fed 30 fishes to three replicates of each group for 8 weeks. In the inflammation model of IECs, the addition of CA (1 mM) remarkably enhanced cell survival, and significantly reduced the secretion of inflammatory cytokines TNF-α and IL-1β (<em>P</em> &lt; 0.05). CA activated phosphorylation of S6K and 4EBP-1 in the mTOR pathway and promoted the protein expression of tight junction proteins (ZO-1, E-cadherin, occludin and claudin-1) (<em>P</em> &lt; 0.05). The supplementation of CA in the diet also could improve the expression of tight junction in intestine of hybrid grouper (<em>P</em> &lt; 0.05). CA attenuated the protein expression of inflammatory cytokines (IL-1β, IL-6 and TNF-α) in inflammation models by regulating ERK and NF-κB pathways (<em>P</em> &lt; 0.05). These findings suggest that citric acid enhances cell survival, strengthens the intestinal tight junction barrier and enhances intestinal immune function in the LPS-stimulated inflammatory model of IECs. Citric acid could serve as an effective means to counteract growth inhibition and reduced digestibility caused by high proportions of plant protein in fish feed.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"46 ","pages":"Article 103282"},"PeriodicalIF":3.7,"publicationDate":"2026-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145734229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beyond genotyping: Using ddRADseq GBS for pathogen surveillance in aquaculture – A case study in Barramundi (Lates calcarifer)","authors":"Celestine Terence ,&nbsp;Zhi Weng Josiah Poon ,&nbsp;Saengchan Senapin ,&nbsp;Ha Thanh Dong ,&nbsp;Shannon M. Clarke ,&nbsp;Rudiger Brauning ,&nbsp;Ken G. Dodds ,&nbsp;Nguyen Thanh Vu ,&nbsp;Xueyan Shen ,&nbsp;Susan Gibson-Kueh ,&nbsp;Dean R. Jerry ,&nbsp;Jose A. Domingos","doi":"10.1016/j.aqrep.2025.103273","DOIUrl":"10.1016/j.aqrep.2025.103273","url":null,"abstract":"<div><div>Genotyping by sequencing (GBS) is widely employed in aquaculture selective breeding programs to acquire SNP genotypes for various applications, including pedigree reconstruction, GWAS, and in the estimation of genetic parameters, genomic relationships and breeding values. Because sequencing libraries encompass all DNA present in fin-clip tissue, they also recover the non-host metagenomic fraction, including pathogens. We leveraged this property to survey for the presence of scale-drop disease virus (SDDV), responsible for 40–90 % mortality in farmed barramundi, while simultaneously genotyping the host. Raw reads from 4484 fish of four commercial cohorts (2239 moribund, 2245 asymptomatic) were aligned to the SDDV reference genome, and viral reads were normalised as reads per million (RPM) per individual. SDDV prevalence and load were tightly associated with clinical status: 88.9 % of moribund fish carried SDDV at 21.8 ± 0.6 RPM, whereas only 0.2 % of healthy fish were positive with 0.002 ± 0.001 RPM. Independent validation by quantitative PCR on fin and spleen from a nested subset of 172 fish (81 moribund, 91 healthy) yielded viral copy numbers strongly correlated with ddRADseq RPM (Spearman’s ρ = 0.84 for fin; ρ = 0.76 for spleen; both P &lt; 0.0001). Viral load was consistently higher in fin (mean 281 ± 45 copies ng⁻¹ DNA) than spleen (147 ± 32 copies ng⁻¹), corroborating the suitability of non-lethal fin tissue for surveillance. Prevalence and load distributions were homogeneous across cohorts, and no qPCR-positive individuals escaped detection by ddRADseq. These findings show that routine ddRADseq datasets in barramundi can also be repurposed into a sensitive epidemiological assay that unites SDDV monitoring with genomic improvement. Further, these findings suggest that breeding programs generating large ddRADseq GBS datasets may also serve pathogen surveillance purposes where the target pathogen infects the host genotyped tissue.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"46 ","pages":"Article 103273"},"PeriodicalIF":3.7,"publicationDate":"2026-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145734278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of dietary lipid sources on growth performance, fatty acid composition, antioxidant capacity, lipid metabolism and intestinal microbiota of Spinibarbus caldwelli","authors":"Xiaofei Cheng ,&nbsp;Hualan Zou ,&nbsp;Jiarong Guo ,&nbsp;Jin Xiang ,&nbsp;Hao Wu ,&nbsp;Zhigang He ,&nbsp;Min Xie ,&nbsp;Yongchao Yuan ,&nbsp;Hong Li ,&nbsp;Cheng Li","doi":"10.1016/j.aqrep.2025.103238","DOIUrl":"10.1016/j.aqrep.2025.103238","url":null,"abstract":"<div><div>The present study aimed to evaluate the influence of varying lipid sources on growth performance, serum biochemical indices, muscle chemical composition, antioxidant capacity, lipid metabolism, and intestinal microbiota composition in <em>Spinibarbus caldwelli</em>. Five experimental diets with equivalent nitrogen (40 % crude protein) and lipid (7 % crude lipid) levels were formulated, incorporating distinct lipid sources: fish oil (FO), soybean oil (SO), rapeseed oil (RO), linseed oil (LO), and palm oil (PO). <em>S. caldwelli</em> (initial weight: 8.02 ± 0.02 g) were randomly allocated into 15 tanks (30 fish/tank; 3 replicates per treatment) and fed twice daily (08:00 and 17:30) for 8 weeks. The results were summarized as follows: 1) The SO, RO, and LO groups exhibited significantly higher weight gain rates (WGR), specific growth rate (SGR), and protein efficiency ratio (PER) than the FO and PO groups (<em>P</em> &lt; 0.05). Notably, the RO group exhibited the lowest feed conversion ratio (FCR). 2) Muscle crude lipid content was significantly elevated in the FO, RO, and LO groups relative to the SO and PO groups (<em>P</em> &lt; 0.05). 3) Monounsaturated fatty acid (MUFA) levels in muscle were markedly higher in both the LO and RO groups (<em>P</em> &lt; 0.05). 4) The SO, RO, and LO groups exhibited reduced hepatic malondialdehyde (MDA) content, while catalase (CAT) and superoxide dismutase (SOD) activities were significantly elevated compared to the FO and PO groups (<em>P</em> &lt; 0.05)<em>.</em> 5) Alpha diversity analysis of the intestinal microbiota revealed that the Shannon and Simpson indices were significantly higher in the SO, RO, and LO groups than those observed in other treatments (<em>P</em> &lt; 0.05). The LO group displayed a predominance of beneficial bacteria; conversely, while exhibiting greater microbial diversity richness than others, the RO group also contained a higher abundance of harmful bacteria (<em>P</em> &lt; 0.05). Furthermore, both the RO and LO groups demonstrated the highest enzymatic activities of fatty acid synthase (FAS), hormone-sensitive lipase (HSL), and carnitine palmitoyltransferase-1 (CPT-1) (<em>P</em> &lt; 0.05). In conclusion, based on growth performance, muscle composition, antioxidant indicators, and microbiota profiles, LO is identified as the optimal lipid source for <em>S. caldwelli</em> diets, followed by FO, RO and SO. PO is not recommended as a dietary lipid source.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"46 ","pages":"Article 103238"},"PeriodicalIF":3.7,"publicationDate":"2026-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145617099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}