Aquaculture Reports最新文献

{"title":"Cloning, expression analysis, and the photoperiodic response of CHH gene in Panulirus homarus","authors":"Zhimin Ju ,&nbsp;Jiajun Luo ,&nbsp;Charles Brighton Ndandala ,&nbsp;Chongqing Wen ,&nbsp;Zhao Yanfei ,&nbsp;Huafang Liang","doi":"10.1016/j.aqrep.2024.102552","DOIUrl":"10.1016/j.aqrep.2024.102552","url":null,"abstract":"<div><div>Two subtypes of the Crustacean Hyperglycemic Hormone (CHH) gene and the cDNA of Panulirus homarus (PhCHHA and PhCHH-B) were been successfully cloned and characterized in this study. The length of PhCHH-A was 1186 bp with a 384 bp open reading frame that encodes a 127 amino acid. The length of PhCHH-B was 662 bp with a 394 bp open reading frame that encodes 131 amino acids. PhCHH-A and PhCHH-B possess most of the characteristics of eyestalk type-I CHH/MIH/GIH family neuropeptides. Both have a “signal peptide, CPRP, and mature peptide” structure. Their mature peptide comprised 74 amino acids and 6 cysteine residues in a conserved position. Homology and phylogenetic analysis showed that PhCHH-A and PhCHH-B were conserved in several functional domains. They showed the highest homology with CHH of Rock lobster (<em>Jasus lalandii</em>). Additionally, high levels of PhCHH-A transcripts were detected in both the intestine and eyestalks, where the expression of PhCHH-B was the highest in eyestalk. In female lobsters, PhCHH-A and PhCHH-B expression levelin eyestalk gradually increased with ovarian development. The photoperiod experiment results showed that ovarian development was optimal at 14 L:10D, and the expression levels of PhCHH-A and PhCHH-B in the eyestalk increased to the peak. In conclusion, these results indicate that PhCHH-A and PhCHH-B might play a critical role in the ovarian developmental cycle of P. homarus. In addition, a photoperiod of 14 L:10D had the most apparent effect on promoting the expression of PhCHH-A and PhCHH-B, and ovarian development.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"40 ","pages":"Article 102552"},"PeriodicalIF":3.2,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143142589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the physiological and biochemical mechanisms underlying growth rate variability in Hong Kong oyster (Crassostrea hongkongensis)","authors":"Na Hu ,&nbsp;Wenhong Li ,&nbsp;Pingping He ,&nbsp;Pinyuan Wei ,&nbsp;Ke Bai ,&nbsp;Junliang Guan ,&nbsp;Li Zhang ,&nbsp;Yongxian Chen ,&nbsp;Wei Li ,&nbsp;Yusi Zheng ,&nbsp;Qingsong He ,&nbsp;Longcheng Liu ,&nbsp;Xingzhi Zhang ,&nbsp;Jinxia Peng","doi":"10.1016/j.aqrep.2024.102570","DOIUrl":"10.1016/j.aqrep.2024.102570","url":null,"abstract":"<div><div>The Hong Kong oyster (<em>Crassostrea hongkongensis</em>), a key aquaculture species in southern China, has significant potential for enhancing profitability through increased growth rate. In this study, we examined the physiological and biochemical characteristics of the large-size (SL) and small-size (SS) from the selected strain, as well as the large-size (CL) and small-size (CS) from the wild population. The results revealed that the oxygen consumption rate (OCR) and ammonia excretion rate (AER) of the SL and CL groups were significantly lower than those of the SS and CS groups (<em>p</em> &lt; 0.05). The O:N ratio in the SL and CL groups ranged from 8.10 to 8.19. In contrast, the SS and CS groups exhibited O:N ratios of 1.17–2.69. Compared with other groups, the SL group showed a significantly higher condition index (2.65 ± 0.22) and scope for growth (59.57 J h^–1 g^–1) (<em>p</em> &lt; 0.05). Significantly elevated activities of α-amylase and lipase (341.71 μg/min/mg prot and 363.83 nmol/min/mg prot) were observed in the SL group compared to the CL and CS groups (<em>p</em> &lt; 0.05). Both digestive enzyme activities (including pepsin, 32.00 U/mg prot), as well as pyruvate kinase and succinate dehydrogenase activities (1.30 nmol/min/mg prot and 7.61 U/mg prot), peaked in the SL group. Our research findings revealed that, compared to the small-size, the large-size within the two populations exhibit lower metabolic costs, higher digestive and absorptive capacities, and efficient ATP production capabilities. Notably, the metabolic efficiency is lowest among the large-size in the selected strain, whereas digestive efficiency and energy utilization rates are at their peak. These discoveries underscore the significance of digestive and metabolic efficiencies in the growth variation of oysters and offer insights into improving aquaculture strategies.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"40 ","pages":"Article 102570"},"PeriodicalIF":3.2,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143142621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined analysis of 16S rRNA sequencing and metabolomics reveals the growth-promoting mechanism of compound probiotics in zig-zag eel (Mastacembelus armatus)","authors":"Yuwei Feng,&nbsp;Zhiting Zuo,&nbsp;Guangting Xie,&nbsp;Yiman Chen,&nbsp;Xiaoli Yin,&nbsp;Baoyue Lu,&nbsp;Zhide Yu,&nbsp;Chong Han,&nbsp;Hu Shu","doi":"10.1016/j.aqrep.2024.102571","DOIUrl":"10.1016/j.aqrep.2024.102571","url":null,"abstract":"<div><div>Probiotics are important natural nutritional immune enhancers in aquaculture. The objective of this experiment was to investigate the effects of single or combined supplementation with marine fish probiotics on growth performance, intestinal physiology, gut microbes, and gut metabolites in zig-zag eel (<em>Mastacembelus armatus</em>)<em>.</em> A total of 1500 three-month-old healthy <em>M. armatus</em> were randomly divided into 5 groups: CON (control group), BC (<em>Bacillus cereus</em> G1–11 group), EA (<em>Exiguobacterium acetylicum</em> G1–33 group), MIX (the compound probiotics group), PC (the commercial probiotics group), and the feeding period was 8 weeks. Growth index and growth-related gene expression were measured to reflect the growth performance of <em>M. armatus.</em> The results showed that the groups supplemented with probiotics promoted the growth of <em>M. armatus</em>, and the MIX had the most significant promotion effect (<em>P</em> &lt; 0.05). The intestinal physiology was reflected by intestinal histology, intestinal digestive enzyme, and antioxidant enzyme activity (<em>P</em> &lt; 0.05). 16S rRNA sequencing was used to analyze the gut microbiota of different groups. The MIX probiotics can promote the activities of intestinal digestive enzymes and antioxidant enzymes by promoting the bacteria <em>Brevundimonas</em>, <em>Bacillus</em>, and <em>Rhodococcus</em> in the intestine. Non-targeted metabolomics was used to analyze intestinal metabolites in each group. The compound probiotics can increase metabolites such as piperazine, phenols, glycerophospholipids, and unsaturated fatty acids. The compound probiotics can increase the number of beneficial intestinal bacteria and beneficial metabolites, promoting intestinal digestion and absorption, maintaining intestinal microbial environment homeostasis, and improving the growth of <em>M. armatus</em>. Overall, this study first provides a comprehensive analysis of the interactions among gut enzyme activity, microbiota, and metabolites in relation to the growth of <em>M. armatus</em>, while also recommending <em>B. cereus</em> G1–11 and <em>E. acetylicum</em> G1–33 for application in freshwater aquaculture.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"40 ","pages":"Article 102571"},"PeriodicalIF":3.2,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143142622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of the policies and constraints limiting the aquaponics industry in Portugal","authors":"Fernando Mata ,&nbsp;Maria dos-Santos","doi":"10.1016/j.aqrep.2024.102572","DOIUrl":"10.1016/j.aqrep.2024.102572","url":null,"abstract":"<div><div>Aquaponics is an innovative and potentially sustainable method of producing vegetables and fish, enabling local, inland fish production, and vegetable production without the need for artificial fertilization. With the aim to analyze the potential development of commercial aquaponics in Portugal, a group of Portuguese stakeholders and experts were interviewed to capture their understanding of the challenges faced by the emerging Portuguese aquaponics sector. The paper evaluates and discusses the main issues experienced by stakeholders, proposing solutions to overcome barriers and restrictions. Legal and administrative barriers were identified, and it is recognized that aquaponics requires its own legal and administrative framework. Bureaucracy is duplicated in licensing processes, authorizations for sale, and permits. Exotic fish species, such as tilapia (Tilapia spp.) face legal barriers to their use in aquaculture. The freshwater aquaculture species to consider for integration into aquaponics systems are largemouth bass (<em>Micropterus salmoides</em>), pikeperch (<em>Sander lucioperca</em>), and eel (<em>Anguilla anguilla</em>). To address barriers to the organic certification of aquaponics products, producers need to collaborate and establish their own certification schemes.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"40 ","pages":"Article 102572"},"PeriodicalIF":3.2,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143142619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maturation of the biofloc system in Penaeus vannamei culture under different salinities and its effects on the microbial communities","authors":"Shaikha Y. Al-Sayegh ,&nbsp;Victor Torres Rosas ,&nbsp;Ponnumony Vethamony ,&nbsp;Nor Azman Kasan ,&nbsp;Hon Jung Liew ,&nbsp;Mhd Ikhwanuddin ,&nbsp;Zulfa Ali Al Disi ,&nbsp;Hadil Elsayed ,&nbsp;Jassim A. Al-Khayat","doi":"10.1016/j.aqrep.2024.102568","DOIUrl":"10.1016/j.aqrep.2024.102568","url":null,"abstract":"<div><div>The purpose of the study is to evaluate the impact of varying salinity levels on bacterial communities that influence the maturation of the biofloc technology (BFT) system in <em>Penaeus vannamei</em> culture. The experiment involved four water treatments with different salinities: seawater from the Arabian Gulf with 44 ppt (M44), seawater diluted to 22 ppt (M22), well water with 22 ppt (W22), and seawater diluted to 11 ppt (M11). The study was conducted in triplicate using a completely randomized design, with a stocking density of 240 shrimps m⁻³ for 45 days until BFT reached a mature state. Water quality parameters were monitored daily, and the bacterial communities in the biofloc system were evaluated every 15 days. The results showed that salinity impacts both the water parameters and the nutritional composition of the biofloc culture. Bacteria that convert ammonia to nitrite matured more rapidly at low salinity (11 ppt), while bacteria that convert nitrite to nitrate matured faster at high salinity (44 ppt). Additionally, this study shows that microbial communities can shift significantly through the maturation process depending on competitive dynamics and the metabolic requirements of bacteria. This study indicates the crucial role of salinity in affecting the microbial community and maturation functionality of the BFT. The results further highlight the significance of preserving a varied and well-balanced bacterial community in biofloc aquaculture systems to optimize nitrification and water quality control.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"40 ","pages":"Article 102568"},"PeriodicalIF":3.2,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143142588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coculture with Eastern oysters is unlikely to reduce OsHV-1 impacts to farmed Pacific oysters: A modelling approach","authors":"Familusi Oluwatosin Adekunle ,&nbsp;Gorka Bidegain ,&nbsp;Tal Ben-Horin","doi":"10.1016/j.aqrep.2024.102567","DOIUrl":"10.1016/j.aqrep.2024.102567","url":null,"abstract":"<div><div>In recent decades, the global expansion of Pacific oyster <em>Magallana gigas</em> aquaculture, driven by this species’ adaptability and rapid growth, has coincided with the emergence of novel and virulent microvariants (<em>mu</em>vars) of Ostreid herpesvirus 1 (OsHV-1), causing significant industry losses. As a potential alternative, the eastern oyster (<em>Crassostrea virginica</em>) demonstrates lower susceptibility to OsHV-1. This study developed a transmission model for OsHV-1 in mixed oyster aquaculture systems, focusing on the impact of <em>C. virginica</em> introductions on transmission dynamics within established <em>M. gigas</em> systems. The model considers oyster growth, mortality, filtration, viral particle release, and environmental decay. In the absence of experimental data on OsHV-1 infection dynamics in co-culture systems, the model was evaluated using data from monoculture systems. Additionally, the co-culture model was validated for mathematical accuracy and behavior across varying species proportions of <em>M. gigas/C. virginica</em> (90/10, 60/40, 50/50, 40/60, 10/90). Simulation results indicated that introducing <em>C. virginica</em> did not impede OsHV-1 progression; even in the <em>M. gigas/C. virginica</em> 10/90 system, <em>M. gigas</em> mortality reached its maximum, albeit delayed by approximately 10 days. Coculture scenarios did not provide a significant advantage for <em>M. gigas</em>, likely due to OsHV-1 particle saturation in the water column. The model’s performance across diverse scenarios positions it as a valuable tool for understanding waterborne pathogen dynamics in mixed host-species systems, supporting investigations into aquaculture species introductions and their influence on disease dynamics.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"40 ","pages":"Article 102567"},"PeriodicalIF":3.2,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143142590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The hypoxia adaptation strategy of the big-belly seahorses (Hippocampus abdominalis) with tuft gills","authors":"Long-Wu Jia ,&nbsp;Le-Le Zhang ,&nbsp;Hong-Wei Yang ,&nbsp;Chun-Yu Hou ,&nbsp;Lin Shi ,&nbsp;Yu-Ping Hou ,&nbsp;Guo-Dong Zheng ,&nbsp;Xiao-Lei Su ,&nbsp;Kai Wang","doi":"10.1016/j.aqrep.2024.102544","DOIUrl":"10.1016/j.aqrep.2024.102544","url":null,"abstract":"<div><div>Hypoxia tolerance is a critical trait in fish breeding, however, the mechanisms underlying hypoxia regulation in the tuft gills of seahorses remain unclear. In this study, we compared the gill structures of the big-belly seahorses (<em>Hippocampus abdominalis</em>, BBS) with lamellae gill fish and evaluated the effects of hypoxia on BBS. The average oxygen tension threshold for loss of equilibrium (LOE_crit) of BBS (2.68 mg·L⁻¹) was higher than that of the common hypoxia-sensitive fish. Compared with the normoxia group, the duration of stationary, irregular swimming, curled tail, lying down behaviors, and respiratory rate significantly (<em>P</em> &lt; 0.05) increased, while the duration of swinging and swimming behaviors significantly (<em>P</em> &lt; 0.05) decreased in response to hypoxia. After 4- or 7-days of hypoxia treatment, the red blood cell counts, SOD, CAT, GSH, and T-AOC of BBS were significantly (<em>P</em> &lt; 0.05) higher than those in the normoxia group. Notably, when BBS were exposed to hypoxia, the relative surface area of the exposed distal gill filaments significantly increased (<em>P</em> &lt; 0.05). Meanwhile, no significant (<em>P</em> &gt; 0.05) changes were observed in the medial and proximal regions. Ultimately, we identified 5 significantly (<em>P</em> &lt; 0.05) down-regulated (<em>itgav</em>, <em>itga6a</em>, <em>itga1</em>, <em>pcna</em>, and <em>adcy9</em>) and 5 significantly (<em>P</em> &lt; 0.05) up-regulated (<em>gusb</em>, <em>src</em>, <em>cdc20</em>, <em>ccnb1</em>, and <em>plk1</em>) core hub genes related to gill remodeling. This study demonstrates that BBS is sensitive to hypoxia, and its unique tuft gills respond to hypoxia through local gill remodel, providing valuable insights for the breeding of hypoxia-tolerance seahorses.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"40 ","pages":"Article 102544"},"PeriodicalIF":3.2,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143142559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Implementing an environmental DNA/RNA-based approach to non-invasively investigate disease caused by Ichthyophthirius multifiliis","authors":"Yajiao Duan,&nbsp;Moonika Haahr Marana,&nbsp;Hannah Malene Jensen,&nbsp;Cyril Henard,&nbsp;Louise von Gersdorff Jørgensen","doi":"10.1016/j.aqrep.2024.102562","DOIUrl":"10.1016/j.aqrep.2024.102562","url":null,"abstract":"<div><div>Infectious diseases impose a substantial burden on aquaculture, acting as a significant constraint on production yields, entailing considerable management costs. White spot disease, which is caused by the protozoan fish parasite, <em>Ichthyophthirius multifiliis</em> (<em>Ich</em>), is a good example of a disease which continuously cause major challenge for the aquaculture worldwide. Utilization of environmental DNA/RNA (eNA) extracted from fish water for pathogen detection and quantification offers a potentially non-invasive and routine strategy for monitoring diseases in aquaculture systems. In this experimental study, rainbow trout (<em>Oncorhynchus mykiss</em>) were challenged with <em>Ich</em> in two concentrations. The eNA was extracted from the tank water and was used to detect the parasite throughout the course of the disease targeting a gene encoding an <em>Ich</em> immobilization antigen (<em>Iag52a</em>). Simultaneously, the parasite burden and immune-relevant gene expression in both water and fish gills were investigated. The results showed that the level of <em>Ich</em> eNA exhibited a peak at the onset of mortality at 10 days post challenge (dpc), decreasing at 12 dpc. Most immune relevant genes expressed in the gills were downregulated at 2 dpc and upregulated at 8 dpc. The gene expression of two acute phase proteins from the host, Serum amyloid A (SAA) and Hepcidin, were investigated both in the gills and in the water. The two genes were downregulated in water at 10 dpc, coinciding with the peak mortality time point and the highest parasite eNA load in the water. This study provides an insight into the pathogen eNA dynamics where a Ct value of 18–25 of <em>iag52a</em> indicated critical disease outbreak.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"40 ","pages":"Article 102562"},"PeriodicalIF":3.2,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143142560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peptide P2 targeting Vibrio parahaemolyticus PirB toxins blocks the cytotoxic effects to shrimp Litopenaeus vannamei","authors":"Xin Zhang ,&nbsp;Ting Chen ,&nbsp;Chunhua Ren ,&nbsp;Yanhong Wang ,&nbsp;Xiao Jiang ,&nbsp;Jiasheng Huang ,&nbsp;Bo Ma ,&nbsp;Boxiang Zhu ,&nbsp;Jiayue Yin ,&nbsp;Chaoqun Hu ,&nbsp;Peng Luo","doi":"10.1016/j.aqrep.2024.102564","DOIUrl":"10.1016/j.aqrep.2024.102564","url":null,"abstract":"<div><div>PirB is the main virulence factor of acute hepatopancreatic necrosis disease (AHPND) which induced the sloughing of hepatopancreatic cells and shrimp death. In addition, <em>Litopenaeus vannamei</em> aminopeptidase N (Lv-APN) is reported to be a functional receptor that mediates the pathogenicity by <em>Vp</em>_{<em>AHPND</em>}. This study aims to screen the PirB binding peptide to rescue the survival rate under <em>Vp</em>_{<em>AHPND</em>} infection. Here, the PirB toxin-binding peptides were selected using the random phage peptide library kit, and their biological function was verified by injecting them under <em>Vp</em>_{<em>AHPND</em>} challenge. The receptor candidates for PirB were genome-wide identified and selected based on gene expression profiles, and the biological function of Lv-APN1 was confirmed through RNAi. Docking analysis of Peptide-Lv-APN1 and PirB-Lv-APN1 was conducted using the MDockPeP server, with comparative analysis implied. As a result, a total of 11 PirB binding peptides were screened, among which P2 was found to effectively improve the shrimp survival rate under <em>Vp</em>_{<em>AHPND</em>} challenge. And knocked down of <em>Lv-APN1</em>, candidate receptor for PirB toxin, rescued mortality under <em>Vp</em>_{<em>AHPND</em>} challenge. Furthermore, docking analysis revealed that the interface of <em>Lv</em>-APN1 to rPirB was consistent with the P2 to rPirB, suggesting that P2 is likely to bind to PirB to block its binding to Lv-APN1 to reduce the mortality. In conclusion, both the injection of P2 and inference of Lv-APN1 can rescue the mortality of shrimp under <em>Vp</em>_{<em>AHPND</em>} challenge, and docking analysis revealed P2 is likely to bind to rPirB, blocking its binding to Lv-APN1 and reducing <em>Vp</em>_{<em>AHPND</em>} infection.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"40 ","pages":"Article 102564"},"PeriodicalIF":3.2,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143142591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and biological characterization of the pathogen responsible for acute haemorrhagic disease in largemouth bass","authors":"Yibin Yang ,&nbsp;Ping Deng ,&nbsp;Hao He ,&nbsp;Qiuhong Yang ,&nbsp;Xiaohui Ai ,&nbsp;Xia Zhu","doi":"10.1016/j.aqrep.2024.102554","DOIUrl":"10.1016/j.aqrep.2024.102554","url":null,"abstract":"<div><div>To investigate the cause of mass mortality in largemouth bass (<em>Micropterus salmoides</em>) at an aquaculture farm in Hubei due to acute hemorrhagic disease, a dominant bacterium named LY02 was isolated from the brain and major internal organs of diseased fishdise. A re-infection experiment confirmed that LY02 was the pathogenic bacterium responsible for the mass mortality. Histopathological studies revealed that the infection caused varying degrees of necrosis and congestion in the brain and major internal organs of the largemouth bass. Polymerase chain reaction (PCR) detection showed that the LY02 isolate carried the toxin genes <em>simA</em>, <em>pdi</em>, <em>sagA</em>, and <em>cfi</em>. Whole-genome analysis identified LY02 as <em>Streptococcus iniae</em>, carrying 330 toxin factor-related genes and 164 resistance factor-related genes. LY02 was found to be highly sensitive to ceftizoxime, cefradine, cefotaxime, azithromycin, erythromycin, enoxacin, doxycycline, and netilmicin; moderately sensitive to norfloxacin and enrofloxacin; and resistant to gentamicin, streptomycin, kanamycin, tobramycin, and neomycin. This study is the first to isolate and identify <em>S. iniae</em> from infected largemouth bass, providing a theoretical basis for the diagnosis and prevention of acute hemorrhagic disease in this species.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"40 ","pages":"Article 102554"},"PeriodicalIF":3.2,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143142561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}