Aquaculture Reports最新文献

{"title":"Effects of dietary distiller's grains on growth and gut microbiota of the sea cucumber Apostichopus japonicus","authors":"Weiyan Li ,&nbsp;Haoran Xiao ,&nbsp;Shufeng Li ,&nbsp;Jiayi Zhang ,&nbsp;Yajie Deng ,&nbsp;Ziyu Liu ,&nbsp;Lingshu Han ,&nbsp;Junxiao Sun ,&nbsp;Yaqing Chang ,&nbsp;Jun Ding","doi":"10.1016/j.aqrep.2025.103057","DOIUrl":"10.1016/j.aqrep.2025.103057","url":null,"abstract":"<div><div>This study evaluated the impacts of dietary distiller's grains (DG) on the growth, nutritional quality, and intestinal microbiota of <em>Apostichopus japonicus</em>. The healthy <em>A. japonicus</em> (21.51 ± 0.64 g initial weight) were allocated to four groups (three replicates each): a basal diet (T0) and three DG-supplemented diets: 10 % powder (T1), 20 % powder (T2), and 5 % liquid (T3), for 60 days trial. Growth parameters, nutrient profiles, and microbial communities were analyzed post-experiment. The group T3 exhibited significantly higher final body weight, weight gain rate, and specific growth rate versus controls (<em>P</em> &lt; 0.05), outperforming T1 and T2. Nutritional analysis revealed elevated crude protein in group T3 (<em>P</em> &lt; 0.05). Electron microscopy demonstrated denser collagen fiber bundles in group T3 compared to T1 and T2. Gut microbiota sequencing indicated distinct structural shifts in group T3, with increased Verrucomicrobia abundance <em>(P</em> &lt; 0.05) and altered community similarity relative to controls (<em>P</em> &lt; 0.05). In comparison, T2 enhanced microbial alpha diversity (<em>P</em> &lt; 0.05), powdered DG (T1 and T2) impaired collagen synthesis and body wall quality despite promoting growth. In contrast, 5 % liquid DG supplementation (T3) optimized growth performance through protein enrichment and beneficial microbial modulation, particularly Verrucomicrobia proliferation. These findings demonstrate that liquid DG enhances <em>A. japonicus</em> aquaculture sustainability by improving growth metrics and nutritional value while modulating the gut microbial structure.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"44 ","pages":"Article 103057"},"PeriodicalIF":3.7,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144917493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salinity modulates gut microbiota and host transcriptome dynamics in juvenile euryhaline fish yellowfin seabream (Acanthopagrus latus)","authors":"Cheng Peng ,&nbsp;Huayi Xue ,&nbsp;Jingjing Zhang ,&nbsp;Jin Zhang ,&nbsp;Jiage Dai ,&nbsp;Yong Zhang ,&nbsp;Shijia Hu","doi":"10.1016/j.aqrep.2025.103075","DOIUrl":"10.1016/j.aqrep.2025.103075","url":null,"abstract":"<div><div>Salinity critically modulates physiological adaptations in aquatic animals, particularly in euryhaline species frequently encountering salinity fluctuations. Here, we investigated how environmental salinity (10 ppt vs. 30 ppt) shapes gut microbiota and host transcriptome interactions in juvenile yellowfin seabream (<em>Acanthopagrus latus</em>; body weight: 7.31 ± 2.54 g). Following a 30-day comparative experiment, fish in brackish water (10 ppt) significantly reduced gut microbiota alpha diversity (Chao1 and Shannon indices, p &lt; 0.05) and enriched <em>Cetobacterium</em> (72.01 % vs. 11.17 % in seawater), whereas seawater enriched <em>Vibrio</em> (31.46 % vs. 0.29 % in brackish water) and <em>Candidatus Arthromitus</em> (29.25 % vs. 1.88 % in brackish water). Gut transcriptomic profiling identified 398 differentially expressed genes, with brackish water fish upregulating metabolism (<em>cpa1</em>, <em>adh1</em>), biosynthesis (<em>cyp46a1</em>), and ion transport (<em>slc26a3</em>), while seawater fish activated immune pathways (<em>ils</em>, <em>ccl20</em>) and redox homeostasis (<em>coq5</em>). Critically, <em>Cetobacterium</em> abundance positively correlated with metabolic genes (<em>dmgdh</em>, <em>adh1</em>, <em>b4galnt1</em>), whereas <em>Vibrio</em> abundance coincided with immune gene expression (<em>vdac2</em>, <em>ils</em>). These re<em>s</em>ults suggest that salinity-driven microbiota-host crosstalk may contribute to environmental adaptation, suggesting potential benefits of brackish water aquaculture.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"44 ","pages":"Article 103075"},"PeriodicalIF":3.7,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144913290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment and characterization of a Gobiocypris rarus cell line for studying type II grass carp reovirus infection","authors":"Wenji Huang ,&nbsp;Sijia Wan ,&nbsp;Ling Shao","doi":"10.1016/j.aqrep.2025.103065","DOIUrl":"10.1016/j.aqrep.2025.103065","url":null,"abstract":"<div><div>The type II Grass Carp Reovirus (GCRV-II) causes severe hemorrhagic disease with high mortality in economically important fish species. However, existing cell culture systems exhibits neither efficient viral replication nor observable cytopathic effects. In this study, a novel cell line (GRLJ) was established utilizing cells obtained from the lower jaw of <em>Gobiocypris rarus</em> (rare minnow), which was characterized in terms of morphology, proliferation, chromosomes, transfection efficiency, susceptibility to GCRV-II, and innate immune response. GRLJ cells with an epithelioid phenotype infected with GCRV-II exhibited a significant viral particle in the cytoplasm, and upregulation of viral genes (<em>vp4</em>, <em>vp56</em>). High-titer infection (1.9 × 10⁴ copies/μL) caused complete lethality in rare minnow by 10 days post-infection (dpi), with evident histopathology. The calculated LD₅₀ for GRLJ-derived GCRV-II was 2.6 × 10 ³ copies/μL. The expression of immune-related genes, such as <em>tlr3</em>, <em>tbk1</em>, <em>ifnα, ifn2</em>, <em>p65</em> and <em>pkr</em> were significantly upregulated at 3 and 7 dpi. These results highlight the potential of GRLJ cells as a model for studying GCRV-II replication and host-pathogen interactions.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"44 ","pages":"Article 103065"},"PeriodicalIF":3.7,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144913291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hepatopancreas transcriptome analysis reveals the adaptation mechanism to high temperature in the clam Cyclina sinensis","authors":"Dehui Sun ,&nbsp;Fengjuan Jiang ,&nbsp;Qing Nie ,&nbsp;Lisha Wei ,&nbsp;Xiuke Ouyang","doi":"10.1016/j.aqrep.2025.103064","DOIUrl":"10.1016/j.aqrep.2025.103064","url":null,"abstract":"<div><div>Temperature is an importantly environmental factor that regulates the growth and survival of clams. To investigate the molecular changes in response to acute high-temperature stress in clam <em>Cyclina sinensis</em>, we conducted a transcriptional profiling. In this study, clams were cultivated at different temperature (26℃, 30℃ and 34℃) for 24 h. Hepatopancreas tissues were then collected for RNA-sequencing. Compared with the CsHe26 group (control group), 339 differentially expressed genes (DEGs) were identified in CsHe30 group containing 176 upregulated DEGs and 163 downregulated DEGs, and 649 DEGs were identified in CsHe34 group including 329 upregulated DEGs and 320 downregulated DEGs. The Go and KEGG analysis showed that DEGs were mainly involved in oxidoreductase activity, protein processing and folding, and mitogen-activated protein kinase (MAPK) signaling pathway. The analysis result of qRT-PCR showed that the expression patterns of 15 DEGs were comparable to the RNA-sequencing data indicating the reliability. Moreover, we found that <em>Caspase3</em> and <em>p38 MAPK</em> were upregulated suggesting increased cell apoptosis. This was further evidenced by the TUNEL staining assay, which showed that high temperature stress increased the number of apoptotic cells in hepatopancreas. Overall, this work provides a valuable data resource for understanding the molecular basis of high-temperature adaptation in clams in the context of global warming and may contribute to identifying heat-tolerant candidate genes which provide potential targets for breeding heat-resistant clam strains.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"44 ","pages":"Article 103064"},"PeriodicalIF":3.7,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144907642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of zinc supplementation on growth, antioxidant status, and physiological health of Cyprinus carpio (Common Carp): Evaluating the optimal dietary zinc requirements","authors":"Shakeela Parveen ,&nbsp;Muhammad Tayyab ,&nbsp;Muhammad Farhan Khan ,&nbsp;Muhammad Hussain ,&nbsp;Noor Fatima ,&nbsp;Javeria Khail ,&nbsp;Youhou Xu ,&nbsp;Peng Zhu ,&nbsp;Laiba Shafique","doi":"10.1016/j.aqrep.2025.103058","DOIUrl":"10.1016/j.aqrep.2025.103058","url":null,"abstract":"<div><div>Zinc is an essential micronutrient in fish that plays vital role in many biological functions. Optimal Zinc level is indispensable in fish feed due to its multifaceted roles specifically in growth, antioxidant capacity and overall aquaculture productivity. The impact of dietary supplementation with zinc methionine (ZM), zinc oxide nanoparticles (ZNPs) and zinc sulfate (ZS) was assessed in juvenile <em>Cyprinus carpio</em>. Fish were fed a basal diet (Control), ZM, ZNPs, and ZS based diets at levels of 10, 20, 30, and 40 mg/kg for 90 days. ZNPs at 20 mg/kg exhibited the maximum weight gain (137.46 ± 1.74 %) and specific growth rate (1.44 ± 0.01 %/day), with lowest feed conversion ratio (1.75 ± 0.021) compared to the ZM and ZS diet groups. Fish fed 20 mg/kg ZNPs showed significantly higher superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) activity, along with reduced liver thiobarbituric acid reactive substances (TBARS). Glucose level was lower in ZNPs groups as compared to other zinc sources, while cortisol levels decreased dose-dependently (p &lt; 0.001). The highest protein level was observed in fish fed 20 mg/kg ZNPs. Serum and muscle zinc concentrations increased in dose dependent manner in the all zinc supplemented dietary groups. Optimal dietary zinc levels were identified for ZM (30.95–35.29 mg/kg), ZNPs (24.13–27.70 mg/kg), and ZS (30.15–33.37 mg/kg) based on growth and health markers. In conclusion, ZNPs improved growth performance, antioxidant capacity, and overall health in <em>C. carpio</em>, making it a promising alternative to traditional zinc supplements.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"44 ","pages":"Article 103058"},"PeriodicalIF":3.7,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144913288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic insights into the effects of Hepatospora eriocheir (Microsporidia) infection in Eriocheir sinensis","authors":"Fanya Nie ,&nbsp;Xiao Zeng ,&nbsp;Xing Lin ,&nbsp;Jinyong Zhang ,&nbsp;Yuanli Zhao","doi":"10.1016/j.aqrep.2025.103066","DOIUrl":"10.1016/j.aqrep.2025.103066","url":null,"abstract":"<div><div>The Chinese mitten crab (<em>Eriocheir sinensis</em>), a key species in China’s freshwater aquaculture, faces growing threats from Hepatopancreatic Necrosis Syndrome caused by the microsporidian parasite <em>Hepatospora eriocheir</em>. Deciphering the molecular mechanisms underlying host-pathogen interactions is essential for developing effective disease control strategies. While alternative splicing (AS) and alternative polyadenylation (APA) represent a critical regulatory mechanism in immune responses to pathogenic challenges, their functional significance in <em>E. sinensis</em> remains poorly characterized. This study employed RNA-seq to analyze hepatopancreas transcriptomes during <em>H. eriocheir</em> infection, systematically investigating differentially expressed genes (DEGs), AS and APA events dynamics. We identified 831 DEGs (|log2FoldChange| <span><math><mo>≥</mo></math></span> 1 and <em>p</em>-value &lt; 0.05) enriched in immune-related pathways including G protein-coupled receptor signaling and epithelial morphogenesis. Additionally, 83 AS events and 914 APA events were detected, demonstrating their coordinated regulatory roles in infection responses. Our analysis revealed APA as a novel post-transcriptional modulator of host immunity, providing critical insights into molecular defense mechanisms against microsporidian invasion. These findings advance understanding of RNA processing regulation in invertebrate innate immunity and establish a framework for targeted disease management in crustacean aquaculture.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"44 ","pages":"Article 103066"},"PeriodicalIF":3.7,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144913289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inclusion of biofloc meal as a mineral source in diets for Peneus vannamei cultured in oligohaline water","authors":"Victor Rosas ,&nbsp;Andrei Basilio Dias ,&nbsp;Plácido Moura ,&nbsp;Jassim A. Al-Khayat ,&nbsp;Marcelo Borges Tesser ,&nbsp;Geraldo Kipper Foes ,&nbsp;Wilson Wasielesky","doi":"10.1016/j.aqrep.2025.103062","DOIUrl":"10.1016/j.aqrep.2025.103062","url":null,"abstract":"<div><div>Biofloc meal (BM) obtained from whiteleg shrimp culture waste is a nutritious ingredient commonly used for its high protein content. Nevertheless, its elevated ash value could be of interest in oligohaline culture, where dissolved ions in water are limited. Five isonitrogenous and isolipidic experimental diets were formulated, consisting of a control diet (BM0, no biofloc meal inclusion) and four other diets (BM2.5, BM5, BM10, and BM20) with increasing BM concentration in each diet, all tested in triplicate. Juveniles from <em>Peneus vannamei</em> (0.40 g) were cultured at a density of 150 shrimps.m⁻³ in oligohaline water (1.22 PSU), for 60 days in a chemoautotrophic biofloc technology (BFT) system. The growth parameters and ionic concentration/ratios were monitored during the experimental period. The results demonstrated significantly higher survival (84.6 %) in the BM20 group and lower survival in the BM0 group (40 %). For all treatments, nitrate (NO₃^--N) reached toxic levels, which may explain the observed mortality. In addition, an increase in water salinity was observed, resulting in a significant accumulation of Sodium (Na⁺) in the treatment BM20. In conclusion, BM conferred protection in whiteleg shrimp against nitrate when cultured in low salinity, resulting in better survival and overall productive parameters for <em>P. vannamei</em>.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"44 ","pages":"Article 103062"},"PeriodicalIF":3.7,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144907641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing temperature, light intensity, and salinity conditions for the culture of an asexual population of Ulva ohnoi","authors":"Zhaojun Zeng ,&nbsp;Wencheng Yang ,&nbsp;Jiawei Liao ,&nbsp;Xinlu Wu ,&nbsp;Manning Lei ,&nbsp;Kun Lin ,&nbsp;Enyi Xie ,&nbsp;Jianjun Cui","doi":"10.1016/j.aqrep.2025.103070","DOIUrl":"10.1016/j.aqrep.2025.103070","url":null,"abstract":"<div><div><em>Ulva ohnoi</em> forms harmful green tides and a large population has recently been recorded in Zhanjiang, China, where its strong growth and reproduction favor its large-scale commercial cultivation. Understanding its biology at different growth stages is essential to maximize its aquaculture potential. We first confirmed the generational identity of our samples to eliminate potential biological variations due to generation type. We used an orthogonal experimental approach to investigate three factors (temperature, light intensity, and salinity) at three different levels, to identify optimal cultivation conditions for each developmental stage, and documented the maturation and early development processes. All of our samples were female gametophytes or apomicts, which produced biflagellate female gametes or apomictic zoids, without undergoing sexual reproduction. The optimal growth conditions for <em>U. ohnoi</em> from Zhanjiang were 20 °C, 90 μmol·m⁻²·s⁻¹ light intensity, and 25 salinity; the optimal reproductive conditions were 25 °C, 60 μmol·m⁻²·s⁻¹ light intensity, and 35 salinity; and the optimal development conditions were 25 °C, 90 μmol·m⁻²·s⁻¹ light intensity, and 25 salinity. Temperature dominated in determining <em>U. ohnoi</em> from Zhanjiang growth and early development, while light intensity was most influential in governing reproduction. Growth, reproduction, and early development were almost unaffected by salinity values between 15 and 35. These findings provide essential baseline information for the large-scale aquaculture of <em>U. ohnoi</em> in the coastal waters of southern China.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"44 ","pages":"Article 103070"},"PeriodicalIF":3.7,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144902981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of background color and aeration on survival, growth performance, skin color change rate, and standard metabolic rate of low quality juvenile pot-bellied seahorses (Hippocampus abdominalis)","authors":"Fengyuan Shen ,&nbsp;Dong Zhang ,&nbsp;Xin Liu ,&nbsp;Xinwei Song","doi":"10.1016/j.aqrep.2025.103069","DOIUrl":"10.1016/j.aqrep.2025.103069","url":null,"abstract":"<div><div>Physical environmental conditions commonly affect the survival and growth performance of cultured seahorses. In addition, due to the differences of broodstock seahorses in quality or physical status (e.g. difference in male’s brood pouch, genes or physical conditions), the quality of juveniles may vary greatly. Under these circumstances, the effects of environmental stressors on juvenile seahorses may become markedly pronounced. Investigating suitable physical environments to improve the survival of low quality juveniles is one of the important issues which need to be considered in seahorse culture. In the present study, we investigated the effects of tank color and aeration intensity on the growth, survival, standard metabolic rate and skin color change rate of the low quality juveniles of the pot-bellied seahorse<em>, Hippocampus abdominalis</em>. The results showed that the juveniles reared in black tank with gentle aeration intensity had the highest survival rate, better growth performance, higher skin color change rate, and lower standard metabolic rate (SMR). Therefore, black background color and gentle aeration are recommended for rearing the low quality <em>H. abdominalis</em> juveniles.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"44 ","pages":"Article 103069"},"PeriodicalIF":3.7,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144902978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of autochthonous live yeasts on survival, growth, digestive functionality, and innate immune response in cobia (Rachycentron canadum) larvae","authors":"Samira Reinoso ,&nbsp;María Soledad Gutiérrez ,&nbsp;Cristóbal Domínguez-Borbor ,&nbsp;Melania Vargas ,&nbsp;Irma Betancourt ,&nbsp;Wilfrido Argüello-Guevara ,&nbsp;Stanislaus Sonnenholzner ,&nbsp;Carlos Alfonso Álvarez-González ,&nbsp;Enric Gisbert ,&nbsp;Paola Navarrete","doi":"10.1016/j.aqrep.2025.103051","DOIUrl":"10.1016/j.aqrep.2025.103051","url":null,"abstract":"<div><div>The use of host-derived probiotic yeasts is a promising strategy to enhance larval performance, but their effects during and after supplementation remain poorly understood. This study evaluated the effects of three autochthonous yeast strains: <em>Candida haemuloni</em> C27, <em>Debaryomyces hansenii</em> C10, and <em>D. hansenii</em> C28, isolated from cobia (<em>Rachycentron canadum</em>). A well-known strain, <em>D. hansenii</em> CBS8339 (isolated from rainbow trout), was included for comparison. Larvae were fed for 14 days (2–15 days post-hatch, dph) with rotifers and <em>Artemia</em> metanauplii enriched with each yeast strain. A total of 75,000 larvae (2 dph; ∼3 mm) were randomly distributed into 15 tanks, with three replicates assigned to each of five groups: three groups received yeasts, one group was supplemented with <em>D. hansenii</em> CBS8339, and one control group received non-enriched live food. Digestive enzyme activity, larval growth, and yeast persistence in the gastrointestinal tract were assessed during (6 and 12 dph) and after (18 and 24 dph) supplementation. Survival and innate immune gene expression were evaluated at 24 dph. The results showed that all strains except <em>D. hansenii</em> C10 remained viable in the larval gut until 24 dph. Larvae fed <em>C. haemuloni</em> C27 and <em>D. hansenii</em> CBS8339 showed higher acid protease activity during supplementation. After supplementation, <em>D. hansenii</em> C28 enhanced the activity of eight out of nine digestive enzymes analyzed, while all yeast-fed groups showed increased α-amylase and lipase activity. <em>C. haemuloni</em> C27 upregulated <em>il-1β</em> and <em>tnf-α</em> expression. Survival was improved in larvae supplemented with <em>C. haemuloni</em> C27 and <em>D. hansenii</em> C28 (∼31 %) compared to the control (∼22 %). No differences in growth or intestinal histology were detected between treatments. These findings demonstrate that <em>C. haemuloni</em> C27 and <em>D. hansenii</em> C28 exert beneficial effects in cobia larvae by enhancing survival, digestive function, and immune response with lasting effects likely due to their persistence in the gut.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"44 ","pages":"Article 103051"},"PeriodicalIF":3.7,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144902979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}