Aquaculture Reports最新文献

{"title":"Oxidative stress and inflammatory responses mediate the high mortality of Takifugu obscurus under combined hypoxia and Vibrio parahaemolyticus stress","authors":"Yakang Song ,&nbsp;Niuniu Deng ,&nbsp;Jingpeng Hou ,&nbsp;Ziyi Wu ,&nbsp;Haoran Lin ,&nbsp;Shuisheng Li ,&nbsp;Danqi Lu","doi":"10.1016/j.aqrep.2025.103071","DOIUrl":"10.1016/j.aqrep.2025.103071","url":null,"abstract":"<div><div>In the complex marine environment, hypoxia stress and bacterial infection pose significant threats to the survival of marine fish. Extensive research has been conducted on the adaptive mechanisms of fish in response to individual hypoxia stress or bacterial infection. However, limited knowledge exists regarding the mechanisms underlying the immune response of fish to the simultaneous stress of hypoxia and bacterial infection. In this study, we investigated the immune response of <em>Takifugu obscurus</em> to the combined stress of hypoxia and <em>Vibrio parahaemolyticus</em> infection. Both in vivo and in vitro experiments were conducted, revealing the upregulation of hypoxia-related genes and their downstream factors in the <em>T. obscurus</em> under hypoxic conditions. Moreover, the expression of inflammatory factors and inflammasome-related genes exhibited significant increases under hypoxia stress, <em>V. parahaemolyticus</em> infection, and the combined stress. Notably, respiratory burst, lactate dehydrogenase (LDH) enzyme activity, lysozyme activity, cell apoptosis, and other indicators demonstrated substantial increases, while antioxidant enzyme activity exhibited a significant decrease. Comparative analysis with individual stressors demonstrated noteworthy alterations in liver injury, cell apoptosis, antioxidant capacity, and expression of inflammatory genes in the <em>T. obscurus</em> under the dual stress. These factors likely contribute to the accelerated mortality observed under combined stress conditions. In conclusion, this study explores the immune response of the <em>T. obscurus</em> to the concurrent stress of hypoxia and <em>V. parahaemolyticus</em> infection, thereby providing novel insights for the advancement of stress resistance and disease control techniques in fish.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"45 ","pages":"Article 103071"},"PeriodicalIF":3.7,"publicationDate":"2025-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145007833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insulin-like androgenic gland factor regulates the development of internal and external secondary sexual characteristics in juvenile male kuruma prawn Marsupenaeus japonicus","authors":"Takehiro Furukawa ,&nbsp;Fumihiro Yamane ,&nbsp;Rino Takeuchi ,&nbsp;Tsuyoshi Ohira ,&nbsp;Kenji Toyota ,&nbsp;Taeko Miyazaki ,&nbsp;Naoaki Tsutsui","doi":"10.1016/j.aqrep.2025.103086","DOIUrl":"10.1016/j.aqrep.2025.103086","url":null,"abstract":"<div><div>In decapod crustaceans, insulin-like androgenic gland factor (IAG) is considered a key regulator of male sexual differentiation and maturation. In the kuruma prawn <em>Marsupenaeus japonicus</em>, an important fishery species in Japan, a homologous molecule, Maj-IAG, has been identified. However, its physiological functions in males remain poorly understood. In this context, we conducted <em>Maj-IAG</em> knockdown (KD) via RNA interference for nine weeks in juvenile male <em>M</em>. <em>japonicus</em>. Double-stranded RNA was administered by injection five times at two-week intervals, with the dosage increased according to juvenile growth. As a result, <em>Maj-IAG</em> KD was successfully achieved, with an average reduction of approximately 65 % in expression level compared with controls at week nine. <em>Maj-IAG</em> KD significantly inhibited the development of the petasma, male-specific external copulatory structure, and parts of the internal reproductive organs, such as the vas deferens and seminal vesicles. In contrast, somatic growth and testicular development were unaffected by <em>Maj-IAG</em> KD. Histological analysis of the testes revealed no evident abnormalities such as degeneration of testicular structures or abnormal arrest of germ cell differentiation. These findings indicate that Maj-IAG partially governs the development of male secondary sexual characteristics in juveniles and further suggest that once testicular differentiation is initiated, the maintenance and development of the testis proceeds without dependence on <em>Maj-IAG</em>. Our study provides fundamental insights that may contribute to the advancement of functional sex reversal technologies in penaeid shrimps.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"45 ","pages":"Article 103086"},"PeriodicalIF":3.7,"publicationDate":"2025-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145007834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Supplementation with nucleotides and seaweed meal enhances immunity, growth performance and resistance to Vibrio parahaemolyticus in pacific white shrimp","authors":"Sergi Segarra ,&nbsp;Juan Diego Calvo-Perez ,&nbsp;Jose Antonio Talledo ,&nbsp;Thanh Chau ,&nbsp;Phuc Hoang ,&nbsp;Loc Tran","doi":"10.1016/j.aqrep.2025.103081","DOIUrl":"10.1016/j.aqrep.2025.103081","url":null,"abstract":"<div><div>Acute hepatopancreatic necrosis disease (AHPND) is a bacterial disease mainly caused by <em>Vibrio parahaemolyticus</em> which produces high mortality rates in Pacific white shrimp (PWS). Dietary nucleotides (NU) and seaweed products contain bioactive compounds with reported benefits in PWS. Our objective was to evaluate the effects of NU, a <em>Macrocystis integrifolia</em> seaweed meal (SM), and their combination on PWS performance and survival upon AHPND challenge. A feeding trial (1250 PWS; 56 days) was performed using diets supplemented with 500 ppm NU (NU500), 500 ppm SM (SM500), 500 ppm NU and 500 ppm SM (NU500SM500), 334 ppm NU and 666 ppm SM (NU334SM666) or no supplementation (Control). A challenge trial (720 PWS; 10 days) was also performed using the same diets plus an additional non-challenged, non-supplemented group. Compared to Control, in the feeding trial all supplemented groups showed significantly higher final mean weight, final biomass, mean weight gain, average daily gain and specific growth rate; and NU334SM666 featured a significantly higher feed intake than all other groups. In the challenge trial, a non-significantly increased survival rate <em>vs</em> the challenged Control (30.63 ± 4.70 %) was seen with NU500 (33.95 ± 9.62 %), SM500 (41.02 ± 3.17 %) and NU500SM500 (45.00 ± 13.54 %), while NU334SM666 achieved a significantly higher survival rate (59.61 ± 9.55 %). Hyaline cells were significantly increased with NU334SM666 in both the feeding and challenge trials. In conclusion, NU and SM exhibit synergistic effects, enhancing PWS immune response and growth performance while reducing post-challenge mortality. These findings support their potential as a sustainable, immunomodulatory nutritional alternative to conventional AHPND management strategies.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"45 ","pages":"Article 103081"},"PeriodicalIF":3.7,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144989835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing fishmeal replacement with tilapia hydrolysate protein: Effects on growth, metabolism, and gene expression in white shrimp","authors":"Huu-The Nguyen ,&nbsp;Yu-Ting Chu ,&nbsp;I.-Pei Kuo ,&nbsp;Zhen-Hao Liao ,&nbsp;Yu-Ru Lin ,&nbsp;Chun-Pei Liao ,&nbsp;Ahnadia Wulan Ramadhana ,&nbsp;Fan-Hua Nan ,&nbsp;Yeh-Fang Hu","doi":"10.1016/j.aqrep.2025.103080","DOIUrl":"10.1016/j.aqrep.2025.103080","url":null,"abstract":"<div><div>Hydrolysate fish protein derived from tilapia by-products (THP) was used to evaluate growth performance in the diet of white shrimp (<em>Penaeus vannamei</em>) and its impact on mTOR signaling pathway and gene expression related to metabolism when partially replacing fishmeal with THP. Four diets were formed, with THP substituting fishmeal levels at 0, 5, 10, and 20 %. The 56-day feeding experiment was conducted with 300 juvenile white shrimp (initial weight: 1.12 ± 0.02 g), divided into four groups. At the end of experiment period, there were no significant differences in survival rates between all of the experimental groups; however, those in the 10 % replacement group exhibited a significant increase (<em>p</em> &lt; 0.05) in growth performance. Additionally, shrimp in the 10 % replacement group showed significant improvements (<em>p</em> &lt; 0.05) in digestive enzyme activity and hemolymph biochemical parameters. In comparison to the control group, the 10 % replacement group had a considerably increased (<em>p</em> &lt; 0.05) crude protein level in shrimp muscle. Furthermore, shrimp in 10 % replacement group showed modifications in structure inside the hepatopancreas and intestinal mucosa. The 10 % THP replacement group exhibited significantly upregulated expression of mTOR pathway-related genes (<em>tor</em>, <em>s6k</em>, <em>4ebp</em>, and <em>akt</em>) as well as key genes involved in amino acid, lipid, glucose, and antioxidant metabolism, indicating enhanced nutrient assimilation, protein synthesis, and cellular metabolic activity in shrimp. Although the 5 % replacement level improved growth performance and expressed genes, the differences were not significant (<em>p</em> &gt; 0.05) compared to the control group. Enhanced growth performance and positive regulation of related genes were most effectively detected at the 10 % replacement level (<em>p</em> &lt; 0.05), whereas the 20 % replacement diet exhibited findings similar to the control group (<em>p</em> &gt; 0.05). As a result, substituting 10 % of fishmeal with THP can boost shrimp growth rates while additionally having a beneficial effect on metabolism and mTOR pathway.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"44 ","pages":"Article 103080"},"PeriodicalIF":3.7,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144932177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impacts of jellyfish homogenates on Atlantic salmon (Salmo salar) gill and head kidney cells","authors":"MD Fast ,&nbsp;CV Veltman ,&nbsp;S.L. Purcell ,&nbsp;R. McCurdy ,&nbsp;D. Maguire ,&nbsp;DB Groman ,&nbsp;S. Andrew ,&nbsp;JD Poley ,&nbsp;B. Jia ,&nbsp;SK Whyte","doi":"10.1016/j.aqrep.2025.103073","DOIUrl":"10.1016/j.aqrep.2025.103073","url":null,"abstract":"<div><div>Gill pathology in farmed Atlantic salmon has become one of the most serious causes of mortality and economic loss. A growing concern in some regions of the industry are jellyfish blooms which have been linked to mortality events. Cytotoxic effects of jellyfish nematocysts/toxins have been described, however, our understanding of impacts of jellyfish ‘stings’ in salmon is limited. Development of <em>in vitro</em> and <em>in vivo</em> models could help elucidate the impacts jellyfish have on salmon and potential contributions to chronic gill pathologies. Jellyfish (<em>Cyanea capillata</em>; <em>Aurelia aurita</em>; <em>Phacellophora camtschatica</em>) were collected from multiple sites and nematocyst homogenates were generated to assess their impacts on Atlantic salmon kidney <em>in vitro</em> and gill cells <em>in vivo</em>. Homogenates contained over 90 identified proteins, characterized by numerous enzymes, structural proteins, toxin precursors, signaling molecules (JNK, ERK, p38), and calcium ion modulators. Filter-sterilized homogenates caused rapid and extensive cytopathic effects in Atlantic Salmon Kidney cells <em>in vitro</em>. This toxicity was (1) dose dependent, (2) consistent across freeze-thaws, and (3) media/cells from treated cultures did not replicate cytopathic effect in new Atlantic Salmon Kidney cell monolayers. A descriptive analysis of histological changes in the gill indicated no major morphologic changes noted in gills, however, consistent minor changes were observed in both the gill arch epithelium and the gill filaments. The greatest impacts following HJN flush were within 3 h and a significant shift between these exposures at 24 h driven by increased expression of healing, redox and inflammatory genes (<em>plekfh1a, mmp13, xrc33, gpx2, timp2-d</em> and <em>gsta</em>) in the unfiltered vs the filter-sterilized nematocyst homogenate samples.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"44 ","pages":"Article 103073"},"PeriodicalIF":3.7,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144988079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A study on the effects of culture salinity and dietary protein levels on growth, nutrient metabolism, and immune response of juvenile grass carp","authors":"Dongyu Huang ,&nbsp;Mingchun Ren ,&nbsp;Shengqi Zhao ,&nbsp;Haifeng Mi ,&nbsp;Tao Teng ,&nbsp;Junjie Qin ,&nbsp;Hualiang Liang ,&nbsp;Lu Zhang","doi":"10.1016/j.aqrep.2025.103082","DOIUrl":"10.1016/j.aqrep.2025.103082","url":null,"abstract":"<div><div>An 8-week experiment was designed to study the effects of culture salinity and dietary protein levels (DPLs) on the growth, nutrient metabolism, and immune response of juvenile grass carp (<em>Ctenopharyngodon idella</em>). Three diets were formulated with different DPLs (31.19 %, 36.03 %, and 40.18 %) and two salinity levels (0 ‰ and 7 ‰) in a 3 × 2 factorial design. 360 grass carp juveniles (initial weight 3.72 g) were distributed into 18 tanks (180 L). The results presented that the growth performance was increased significantly by 36.03 % DPLs at 0 ‰ salinity, and 40.18 % DPLs at 7 ‰ salinity, respectively. Regarding nutrient metabolism, at 0 ‰ salinity, 36.03 % DPL up-regulated the mRNA expression levels of <em>igf-1</em>, <em>s6k</em>, and <em>fas</em>, and 40.18 % DPL up-regulated the mRNA expression levels of <em>gk</em>; at 7 ‰ salinity, compared to the 31.19 % DPL group, 36.03 % and 40.18 % DPLs significantly up-regulated the mRNA expression levels of <em>s6k1</em>. Furthermore, compared to 0 ‰ salinity, the <em>gk</em>, <em>g6pase</em>, <em>fas</em>, and <em>cpt1</em> mRNA expression levels were significantly up-regulated at 7 ‰ salinity. Regarding antioxidant capacity, at 0 ‰ salinity, 36.03 % DPL obtained the maximum CAT and SOD activities, and the minimum MDA content; at 7 ‰ salinity, 36.03 % and 40.18 % DPLs obtained the maximum CAT activities. Furthermore, compared to 0 ‰ salinity, the CAT activities and GSH contents were increased, and the MDA contents were decreased significantly at 7 ‰ salinity. Regarding immune response, at 0 ‰ salinity, the <em>stat3</em> mRNA expression level was significantly decreased in the 36.03 % DPL group, and the <em>nfκb</em> and <em>stat3</em> mRNA expression levels were significantly decreased in the 36.03 % and 40.18 % DPLs at 0 ‰ salinity. Compared to 0 ‰ salinity, the <em>stat3</em> mRNA expression levels were significantly down-regulated at 7 ‰ salinity. Furthermore, appropriate protein levels (36.03 % and 40.18 %) and 7 ‰ salinity also significantly improve the immune response.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"44 ","pages":"Article 103082"},"PeriodicalIF":3.7,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144925106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ferulic acid improved lipid metabolism of blunt snout bream, Megalobrama amblycephala juveniles via the intestinal microbiota and metabolites","authors":"Yan Lin ,&nbsp;Xiangjun Leng ,&nbsp;Xiaoqin Li ,&nbsp;Linjie Qian ,&nbsp;Siyue Lu ,&nbsp;Qiaoqiao Mu ,&nbsp;Zhengyan Gu ,&nbsp;Wenqiang Jiang ,&nbsp;Linghong Miao ,&nbsp;Xianping Ge","doi":"10.1016/j.aqrep.2025.103059","DOIUrl":"10.1016/j.aqrep.2025.103059","url":null,"abstract":"<div><div>Fish is an important source of protein for human, and lipid homeostasis is essential for fish health. As a green plant extract, ferulic acid (FA) plays a crucial role in modulating lipid metabolism. This study investigate the effects of dietary FA supplementation on lipid metabolic homeostasis, intestinal microbiota, and metabolites in plasma and intestinal contents of <em>Megalobrama amblycephala</em> juveniles. <em>M. amblycephala</em> (average weight: 9.75 ± 0.04 g) juveniles were fed with three feeds containing 0 (Control), 100 (FA100), and 200 (FA200) mg/kg FA with equivalent nitrogen and energy levels for 8 weeks. The results indicate that FA supplementation significantly reduce the content of plasma lipids and liver lipids, and markedly increase total bile acid content in liver. It is possible that FA regulates liver fatty acid metabolism and cholesterol-bile acid metabolism by up-regulating genes such as carnitine palmitoyl transferase 1 A, cholesterol 7α-hydroxylase, and down-regulating genes such as sterol regulatory element-binding protein. The diversity of the intestinal microbiota is significantly enhanced, while the abundance of harmful bacteria exhibits a marked reduction in FA100 group. Further, metabolites are altered in FA100 group. Epoxy eicosatrienoic acid is a co-upregulated metabolite in both intestinal contents and plasma, and was significantly correlated with multiple indicators related to lipid metabolism. In summary, supplementing FA to feed decrease liver and plasma lipid accumulation and maintained lipid metabolic homeostasis. This may be due to the changed intestinal microbiota of <em>M. amblycephala</em>, as well as enrichment of intestinal and plasma metabolites.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"44 ","pages":"Article 103059"},"PeriodicalIF":3.7,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144925103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of extrusion moisture and temperature on digestibility of barramundi (Lates calcarifer) diets containing bacterial protein meal","authors":"R. Le Boucher,&nbsp;W. Chung,&nbsp;C.S. Lee,&nbsp;C. Wu,&nbsp;L.S.E. Tan,&nbsp;J.K. L. Ng,&nbsp;B.W.R. Neo,&nbsp;T.H. Ng","doi":"10.1016/j.aqrep.2025.103063","DOIUrl":"10.1016/j.aqrep.2025.103063","url":null,"abstract":"<div><div>Bacterial protein meals (BPMs) are emerging as promising alternatives to conventional protein sources in aquafeeds. However, clear limitations have also been evidenced regarding the high inclusion rate of BPMs in aquafeed. This study investigates how feed extrusion settings can improve digestibility of diets containing a BPM derived from bacteria cultivated on brewery by-products in barramundi (<em>Lates calcarifer</em>). Over 36 days, 2640 barramundi (85.9 ± 1.6 g) were reared in 24 tanks (1000 L) and fed 8 experimental diets. These diets were extruded at either 100 °C or 140 °C, with barrel moisture levels of 33 % or 40 %, and included either 0 % (CTL diet) or 30 % BPM (BPM diet), replacing 50 % of the soy-based ingredients. Fish performance, diet apparent digestibility coefficients (ADCs) for macronutrients, 42 fatty acids and 17 amino acids, digestive enzyme activity, and gut transcriptomics were assessed. BPM inclusion did not impact feed intake but reduced growth, lowered the ADCs, and increased feed conversion ratio. However, the extrusion settings specifically improved BPM diet digestibility (P &lt; 0.05) without affecting CTL diet. Extruding the BPM diet at 140 °C with a 33 % moisture rate showed the greatest benefits for energy digestibility. The activity of digestive trypsin, chymotrypsin, and leucine aminopeptidase, remained unaffected by BPM incorporation (P &lt; 0.05), suggesting no anti-nutritional factors in BPM. The lower digestibility of the BPM diet likely stems from reduced nutrient absorption, supported by transcriptomic analyses. These revealed 58 upregulated and 146 downregulated genes (P &lt; 0.1) in the midguts of BPM diet-fed fish, indicating changes in lipid and amino acid metabolism, oxidative phosphorylation, and chemokine signaling. By controlling extrusion temperature and moisture, it was possible to enhance the nutrient digestibility of a diet containing 30 % BPM. The study also revealed the impact of interactions between extrusion parameters and feed formulation on nutrient digestibility.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"44 ","pages":"Article 103063"},"PeriodicalIF":3.7,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144925102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular mechanisms of infertility in male triploid pacific oysters (Crassostrea gigas)","authors":"Xiumei Liu ,&nbsp;Yancheng Zhao ,&nbsp;Jidong Zhang ,&nbsp;Enshuo Zhang ,&nbsp;Yanwei Feng ,&nbsp;Zan Li ,&nbsp;Weijun Wang ,&nbsp;Jianmin Yang","doi":"10.1016/j.aqrep.2025.103074","DOIUrl":"10.1016/j.aqrep.2025.103074","url":null,"abstract":"<div><div>The development of triploid oysters has resolved supply disruptions linked to the spawning season, allowing oysters to be available throughout the year. However, recent statistics indicate a yearly increase in fertilization occurrences among triploid oysters, potentially leading to the eventual loss of their sterility. This development could have significant implications for the entire oyster aquaculture industry. Therefore, we aim to explore the underlying causes of triploid fertility by identifying key genes through transcriptomic analysis. During our investigation and sampling, we observed a yearly increase in the proportion of fertile triploid male <em>Crassostrea gigas</em>. The main goal of this research is to explore the variations between fertile and infertile triploid male <em>C. gigas</em>. We performed transcriptomic analysis on fertile and infertile triploid male <em>C. gigas</em>, identifying 3016 upregulated DEGs and 1209 downregulated DEGs, respectively. Functional enrichment of these DEGs indicated that the majority of GO terms and KEGG pathways were linked to reproductive processes. Subsequently, we constructed a PPI network based on KEGG pathways related to fertility. We ultimately validated 23 important genes using qPCR, observing consistent downregulation in the infertile triploid male group. The primary cause of infertility was the suppression of gene expression regulating germ cell proliferation, disruption of the mitotic cycle, and failure to produce germ cells. These findings provide a crucial foundation for exploring strategies to reduce the reproductive capability of triploid <em>C. gigas</em>, thereby promoting the breeding of high-quality triploid <em>C. gigas</em>.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"44 ","pages":"Article 103074"},"PeriodicalIF":3.7,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144925104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Lactobacillus plantarum supplementation on growth performance, digestive enzyme activity, antioxidant capacity, immune response, and intestinal health in red claw crayfish (Cherax quadricarinatus)","authors":"Qin Zhang ,&nbsp;Luoqing Li ,&nbsp;Haoliang Lu ,&nbsp;Liuqing Meng ,&nbsp;Qinghui Zeng ,&nbsp;Rui Wang ,&nbsp;Dapeng Wang ,&nbsp;Tong Tong ,&nbsp;Yongqiang Liu ,&nbsp;Huizan Yang","doi":"10.1016/j.aqrep.2025.103077","DOIUrl":"10.1016/j.aqrep.2025.103077","url":null,"abstract":"<div><div>The objective of the present study was to evaluate the effects of gradient addition of <em>Lactobacillus plantarum</em> to feed on growth performance, digestive enzyme activities, antioxidant capacity, immune response, and intestinal health of red claw crayfish. The experiment comprised four distinct treatment groups: control group (CK, 0 g·kg⁻¹), low concentration group (LL, 0.10 g·kg⁻¹), medium concentration group (ML, 1.00 g·kg⁻¹), and high concentration group (HL, 10.00 g·kg⁻¹). Three biological replicates were established in each group, with each replicate containing 50 juvenile crayfish at the beginning of the experiment, with initial body weights ranging from 0.13 ± 0.01 g. The experiment spanned 70 days. The experimental results showed that different concentrations of <em>Lactobacillus plantarum</em> significantly increased (<em>p</em> &lt; 0.05) the weight gain rate, specific growth rate, length gain rate, hepatosomatic index, survival rate, and intestinal morphology in red claw crayfish. Additionally, the treatment significantly enhanced (<em>p</em> &lt; 0.05) the activities of lipase, tryptase, and α-amylase in the intestine and hepatopancreas, and significantly up-regulated (<em>p</em> &lt; 0.05) the expression of key genes in the hepatopancreas related to antioxidant defense, immunity, and stress response, including superoxide dismutase, glutathione peroxidase, glutathione, catalase, anti-lipopolysaccharide factor, crustacean hyperglycemic hormone, serine protease inhibitors, lysozyme, alkaline phosphatase, down syndrome cell adhesion molecule, antibacterial peptides, and heat shock protein 70. In conclusion, the addition of <em>Lactobacillus plantarum</em> to the feed of red claw crayfish significantly enhanced its growth performance, digestive enzyme activities, antioxidant capacity, and immune response capacity and positively affected its intestinal health. The optimum addition of <em>Lactobacillus plantarum</em> under this experiment was determined to be 1.00 g·kg⁻¹ .</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"44 ","pages":"Article 103077"},"PeriodicalIF":3.7,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144925105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}