Aquaculture Reports最新文献

{"title":"Organic rare earth feed additive to enhance Penaeus monodon’s muscle quality: A comprehensive insights into texture, amino acid, and fatty acid composition","authors":"Jiaoping Yu ,&nbsp;Wanqi Wang ,&nbsp;Guowei Liao ,&nbsp;Zhen Zhang ,&nbsp;Yuanyuan Yao ,&nbsp;Kaimin Cheng ,&nbsp;Jun Hu ,&nbsp;Yalin Yang ,&nbsp;Zhigang Zhou ,&nbsp;Lanfen Fan","doi":"10.1016/j.aqrep.2024.102585","DOIUrl":"10.1016/j.aqrep.2024.102585","url":null,"abstract":"<div><div>This study investigated the effects of dietary organic rare earth feed additive (OREFA) on the muscle quality of <em>Penaeus monodon</em> (initial weight 2.09 ± 0.01 g). Shrimp in each group were fed with basal diets supplemented with 0(CON), 0.1(OREFA1), 0.2(OREFA2), and 0.3 g/kg (OREFA3) of OREFA for 56d, separately. The results revealed that the OREFA2 group showed significantly higher final body weight, weight gain and specific growth rate compared to the CON group, while exhibiting a significantly lower feed conversion rate (<em>P</em> &lt; 0.05). Additionally, the OREFA1, OREFA2 and OREFA3 groups demonstrated significantly improved adhesiveness, chewiness and elasticity compared to the CON group of muscle (<em>P</em> &lt; 0.05). Furthermore, compared to the CON group, the OREFA2 group exhibited a significantly increase in the content of umami amino acids (glycine), monounsaturated fatty acids (C18:1, C18:1(n-9), C20:1, C22:1, C24:1) and polyunsaturated fatty acids (C18:2, C18:3, C20:2, C20:4, C20:3, C20:5, C22:4, C22:6) (<em>P</em> &lt; 0.05). Additionally, the relative expression levels of muscle growth-related genes <em>PI3K</em> (Phosphoinositide 3-kinase), <em>TOR</em> (target of rapamycin), <em>AKT</em> (Protein kinase B), <em>S6K1</em> (ribosomal protein S6 kinase 1) and <em>4E-BP1</em> (4E-binding protein 1) were significantly higher in shrimp muscle compared to the CON group (<em>P</em> &lt; 0.05). These results indicate that OREFA not only enhanced growth performance, but also improved muscle quality in <em>P. monodon</em> by optimizing amino acid and fatty acid composition, and promoting the expression of muscle growth-related genes. This study provided effective evidence for the application of OREFA supplementation in producing healthy, high-quality and nutritionally enhanced <em>P. monodon</em>.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"40 ","pages":"Article 102585"},"PeriodicalIF":3.2,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143142224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Enteromorpha prolifera polysaccharide on intestinal health of spotted sea bass Lateolabrax maculatus","authors":"Zhongying Long ,&nbsp;Longhui Liu ,&nbsp;Huihui Qin ,&nbsp;Sishun Zhou ,&nbsp;Lumin Kong ,&nbsp;Jianrong Ma ,&nbsp;Hao Lin ,&nbsp;Zhangfan Huang ,&nbsp;Yi Lin ,&nbsp;Zhongbao Li","doi":"10.1016/j.aqrep.2024.102584","DOIUrl":"10.1016/j.aqrep.2024.102584","url":null,"abstract":"<div><div><em>Enteromorpha prolifera</em> polysaccharide (EPP) is a kind of seaweed polysaccharide with outstanding biological activity. In this study, a 49-day culture trial was conducted to explore the effects of dietary EPP on intestinal health in spotted sea bass. Six experimental diets containing 0, 3, 6, 9, 12, and 15 g/kg EPP were formulated. A total of 540 similarly sized and healthy spotted sea bass were randomly assigned to 6 groups and fed the respective diet. The results showed that dietary EPP supplementation significantly improved the activity of digestive enzymes, including amylase, lipase, and trypsin, and reduced the permeability of the intestinal mucosa, which was shown to significantly reduce the content of D-lactic acid and the activity of diamine oxidase in serum. On the other hand, intestinal villi length and muscle thickness increased significantly, and intestinal tissue morphology improved. Dietary EPP also changed intestinal microbial composition, increased <em>Firmicutes</em> abundance, and increased intestinal microbial diversity. Transcriptome results suggest that EPP may improve intestinal tissue morphology and intestinal barrier function by up-regulating tight junction protein 1 (TJP1) and zona occludens 1 (ZO-1) genes, thereby improving intestinal health. In addition, significant changes in several inflammatory factor genes were observed. EPP may have enormous potential for regulating immune and inflammatory responses. In conclusion, dietary EPP can significantly improve the intestinal health of spotted sea bass.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"40 ","pages":"Article 102584"},"PeriodicalIF":3.2,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143142664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Brain transcriptome analysis of snakehead (Channa argus) under starvation and satiation conditions and identification of differentially expressed gene response to feeding regulation","authors":"Xiao-yan Jin ,&nbsp;Xiu-mei Chen ,&nbsp;Gui-liang Guo ,&nbsp;Li Sun ,&nbsp;Xue-qin Wu ,&nbsp;Yun-jie Lin ,&nbsp;Xiao-tian Niu ,&nbsp;Yi-di Kong ,&nbsp;Min Li ,&nbsp;Gui-qin Wang","doi":"10.1016/j.aqrep.2024.102581","DOIUrl":"10.1016/j.aqrep.2024.102581","url":null,"abstract":"<div><div>The <em>Channa argus</em> (<em>C. argus</em>), a warm-water piscivorous fish, is an important economic breed with high nutritional value, but there are some problems such as difficulty in domestication and feeding. In order to solve the problem of feeding and to better understand the regulation of feeding at the molecular level, RNA sequencing (RNA-seq) was sequenced in the brains of wild and cultured <em>C. argus</em> under starvation and satiation. The groups included the wild <em>C. argus</em> starvation group (WST), the wild <em>C. argus</em> satiation group (WSA), the cultured <em>C. argus</em> starvation group (CST) and the cultured <em>C. argus</em> satiation group (CSA). For the pairwise comparisons of these four groups, the number of differential genes was 43 (WST <em>vs</em>. WSA, 16 up-regulated and 27 down-regulated genes), 96 (CST <em>vs</em>. CSA, 44 up-regulated and 52 down-regulated), 193 (WST <em>vs</em>. CST, 31 up-regulated and 162 down-regulated genes), 489 (WSA <em>vs</em>. CSA, 256 up-regulated and 233 down-regulated genes), respectively. Differential gene functional enrichment analysis revealed that several pathways were highlighted, including PI3K-Akt signaling pathway, adipocytokine signaling pathway, estrogen signaling pathway, melanogenesis, and cyclic adenosine monophosphate (cAMP) signaling pathway in the cultured starvation group and the cultured satiation group. In the adipocytokine signaling pathway, down-express gene is agouti-related peptide (AgRP), the cAMP signaling pathway showed up-regulated genes such as chromogranin A (CGA), luteinizing hormone beta polypeptide (LHB), thyroid stimulating hormone beta (THSB), and follicle stimulating hormone beta (FSHB). These pathways and genes may play important roles in the regulation of energy metabolism and feeding. Quantitative real-time PCR of the starvation, satiation, and lipopolysaccharide (LPS) treatment groups showed that the differential expression changes of appetite-related and immune-related genes matched the trend of differential expression of the transcriptome counterparts. This study provides the basic data for the regulation of feeding and the molecular mechanisms related to feeding.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"40 ","pages":"Article 102581"},"PeriodicalIF":3.2,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143142623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of the intestinal flora of Siniperca chuatsi at different growth stages under three feeding practices","authors":"Yunyun Yan ,&nbsp;Fubao Wang ,&nbsp;Xiao Chen ,&nbsp;Junjian Dong ,&nbsp;Fengying Gao ,&nbsp;Hetong Zhang ,&nbsp;Xing Ye ,&nbsp;Chengbin Wu ,&nbsp;Chengfei Sun","doi":"10.1016/j.aqrep.2024.102582","DOIUrl":"10.1016/j.aqrep.2024.102582","url":null,"abstract":"<div><div>The intestinal flora plays a key role in digestion and nutrient absorption, and its composition in fish can vary depending on feeding practices and growth stages. <em>Siniperca chuatsi</em> feeds on live bait throughout its life, and preliminary developments have been made for compound feed as an alternative to live bait in aquaculture. We investigated changes in the intestinal flora of <em>S. chuatsi</em> under three different feeding practices (live bait, compound feed, and mixed diet) at different growth stages by 16S rRNA sequencing. A total of 4,220,390 valid sequences were obtained, clustered into 9103 ASVs. The intestinal flora of <em>S. chuatsi</em> was dominated by Pseudomonadota, Firmicutes, and Bacteroidota. With growth, the α-diversity of the intestinal flora in the live bait group and mixed diet group significantly decreased (<em>p</em> &lt; 0.05), while the α-diversity in the compound feed group did not change significantly (<em>p</em> &gt; 0.05). There were no significant differences in α-diversity among the feeding practices at either one month or two months of age (<em>p</em> &gt; 0.05). At three months of age, the α-diversity was significantly higher in the compound feed group compared to the mixed diet group or live bait group (<em>p</em> &lt; 0.05). Functional prediction revealed that the carbohydrate metabolism pathway was most enriched in the compound feed group, while the amino acid metabolism pathway was most enriched in the live bait group. In the mixed diet group, both the amino acid metabolism and carbohydrate metabolism pathways were similarly enriched. A relatively high number of marker genera were observed in the compound feed group, of which <em>Stenotrophomonas</em> and <em>Cetobacterium</em> are potentially valuable in the application of feed additives for <em>S. chuatsi</em>. This study provides insights into the adaptation mechanisms of intestinal flora to compound feed, supporting diet domestication and the optimization of compound feed formulation for <em>S. chuatsi</em>.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"40 ","pages":"Article 102582"},"PeriodicalIF":3.2,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143142666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and expression analysis of the heat shock proteins Hsp70, Hsp90, and Hsp90b in Litopenaeus vannamei under low-temperature stress","authors":"Min Peng ,&nbsp;Hui Wang ,&nbsp;Shuiping Wen,&nbsp;Zheng Liang,&nbsp;Zheng Huang,&nbsp;Bin Zhang,&nbsp;Tianchong Chen,&nbsp;Qingyun Liu,&nbsp;Qiangyong Li,&nbsp;Yuan Meng,&nbsp;Yuliu Huang,&nbsp;Chunling Yang,&nbsp;Digang Zeng,&nbsp;Manyuan Li,&nbsp;Weilin Zhu,&nbsp;Yongzhen Zhao","doi":"10.1016/j.aqrep.2024.102591","DOIUrl":"10.1016/j.aqrep.2024.102591","url":null,"abstract":"<div><div><em>Litopenaeus vannamei</em> is a tropical and subtropical species of shrimp, and its growth, physiology, and immune functions are affected by low temperatures. This study investigated the tissue-specific expression patterns of the heat shock protein genes <em>hsp70</em>, <em>hsp90</em>, and <em>hsp90b</em> in <em>L. vannamei</em> under low-temperature stress, and their correlation with cold tolerance. Fluorescence in situ hybridization revealed widespread expression of <em>hsp70</em>, <em>hsp90</em>, and <em>hsp90b</em> in the nucleus and cytoplasm of hepatopancreatic tubular cells. Notably, the expression of <em>hsp70</em> was significantly higher in a cold-tolerant family of <em>L. vannamei</em> compared to a common family at 28 °C. The expression level of <em>hsp90</em> was considerably elevated in the cold-tolerant family following exposure to low temperatures (18 °C and 10 °C) compared to that of the common family. During low-temperature stress, <em>hsp70</em> and <em>hsp90</em> expression levels increased gradually in both families, while <em>hsp90b</em> expression decreased. Downstream genes regulated by <em>hsp90</em>, such as <em>akt</em>, were initially upregulated, but then became downregulated, under low-temperature stress. RNA interference-mediated knockdown of <em>hsp90</em> affected PI3K/AKT pathway signaling, which downregulated the downstream genes <em>akt</em> and apoptosis-inhibiting <em>bcl-xl</em>, <em>bcl-2</em>, and <em>creb</em>, and upregulated the apoptosis-promoting genes <em>casp1</em> and <em>casp9</em>. Additionally, the survival rate of <em>L. vannamei</em> significantly decreased following <em>hsp90</em> knockdown. DNA sequence alignment identified 12 single nucleotide polymorphisms (SNPs) in the <em>hsp70</em> gene and 17 SNPs in the <em>hsp90</em> gene. Linkage disequilibrium analysis revealed that eight SNPs in <em>hsp70</em> were significantly associated with low-temperature tolerance (<em>P</em> &lt; 0.01), and 4 SNPs were moderately associated with low-temperature tolerance (<em>P</em> &lt; 0.05). All 17 SNPs in <em>hsp90</em> were significantly associated with low-temperature tolerance (<em>P</em> &lt; 0.01). These findings highlight the critical role of heat shock proteins in the low-temperature response of <em>L. vannamei</em> and provide valuable insights into understanding the mechanisms of shrimp cold tolerance and the development of cold-tolerant varieties.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"40 ","pages":"Article 102591"},"PeriodicalIF":3.2,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143142663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Incorporation of reduced glutathione to the extender improves frozen-thawed sperm function and fertility potential in mandarin fish (Siniperca chuatsi)","authors":"Sen Yang ,&nbsp;Wenjie Xu ,&nbsp;Yanglin Hua ,&nbsp;Qingxin Ruan ,&nbsp;Zining Meng","doi":"10.1016/j.aqrep.2024.102590","DOIUrl":"10.1016/j.aqrep.2024.102590","url":null,"abstract":"<div><div>Semen cryopreservation is a valuable tool for assisted reproduction in aquatic species. Despite the various advantages in hatchery practices, damage occurred during the process of freezing and thawing can result in compromised sperm quality. Supplementation of antioxidant in the freezing medium is suggested to be an effective strategy against cryodamage. Therefore, the objective of this study was to investigate the potential effects of reduced glutathione (GSH) on post-thaw sperm quality and fertility in mandarin fish (<em>Siniperca chuatsi</em>). Semen was diluted with freezing medium at a ratio of 1:6 (v/v), with GSH supplemented at different concentrations (0, 1, 2, 4, 8 mM) and then subjected to cryopreservation. Sperm motion parameters, viability, mitochondrial activity, lipid peroxidation, and apoptosis were assessed after thawing. The addition of GSH at 4 mM presented significantly higher (<em>P</em> &lt; 0.05) post-thaw sperm total motility (%, 71.7 ± 7.2 vs. 64.6 ± 2.2), progressive motility (%, 14.9 ± 2.8 vs. 10.7 ± 0.4), curvilinear velocity (μm/s, 59.6 ± 4.5 vs. 51.2 ± 0.9), straight line velocity (μm/s, 25.3 ± 2.4 vs 22.1 ± 0.5), and average path velocity (μm/s, 37.3 ± 3.6 vs. 32.7 ± 0.6), compared to the control group. Viability was higher (<em>P</em> &lt; 0.05) in sample supplemented with 2 mM GSH (82.0 ± 5.4 %) compared to other groups. In addition, GSH-supplemented samples showed significantly higher mitochondrial activity and a lower level of apoptosis (<em>P</em> &lt; 0.05) compared to the control. Regarding lipid peroxidation, the rate was lower in treatments supplemented with GSH, although not significant (<em>P</em> &gt; 0.05). After artificial insemination, a higher fertilization rate was recorded with GSH at concentrations of 2 and 4 mM (<em>P</em> &lt; 0.05). Moreover, a significantly higher hatching rate (<em>P</em> &lt; 0.05) was observed in the 2 mM GSH-treated group. In conclusion, the addition of GSH seems to provide a cryoprotective effect for mandarin fish sperm, resulting in improved post-thaw sperm quality and fertilizing capacity.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"40 ","pages":"Article 102590"},"PeriodicalIF":3.2,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143142670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimal protein requirement for rapid and coordinated test growth of juvenile sea urchin (Strongylocentrotus intermedius)","authors":"Panke Gong ,&nbsp;Haijing Liu ,&nbsp;Dan Gou,&nbsp;Weixiao Di,&nbsp;Huinan Zuo,&nbsp;Jun Ding,&nbsp;Yaqing Chang,&nbsp;Rantao Zuo","doi":"10.1016/j.aqrep.2024.102578","DOIUrl":"10.1016/j.aqrep.2024.102578","url":null,"abstract":"<div><div>Rapid and coordinated test (the main part of shell) growth is crucial for maintaining the normal body shape of juvenile sea urchin (<em>Strongylocentrotus intermedius</em>). However, imbalanced growth of the test height (TH) and test diameter (TD) of <em>S. intermedius</em> frequently occurs when they are fed formulated feeds. In this study, a 90-days satiety feeding experiment was performed to investigate the effects of different protein levels on the feed intake, somatic growth, and test characteristics of juvenile <em>S. intermedius</em>. Fresh kelp (<em>Saccharina japonica</em>) (control diet) and five formulated feeds with graded levels of protein (5 %, 10 %, 15 %, 20 %, and 25 %) were used as experimental diets, which were named kelp, P5, P10, P15, P20, P25 groups. Each diet was randomly allocated to three cages of sea urchins (initial body weight: 1.23 ± 0.01 g). The results indicated that feed intake gradually decreased as dietary protein increased. The weight gain rate of <em>S. intermedius</em> in the P10 group was comparable to that in the P5 and P15 groups but was significantly higher than that in the P20 and P25 groups. The test height growth rate of <em>S. intermedius</em> in the P5 group was comparable to that in the P10 and P15 groups but was still lower than that in the kelp group. The TH/TD of <em>S. intermedius</em> in the P5 group was comparable to that in the P10 and kelp groups but was significantly greater than that in the P15, P20, P25 groups. <em>S. intermedius</em> in the P5 group showed the closest test microstructure to those in the kelp group, characterized by larger holes, longer but thinner trabeculae, as well as more regular microstructure in both outer and side surfaces, and more active resorption of inner test surface. These results indicated that <em>S. intermedius</em> fed diets with 5 % protein had fastest growth of TH and most balanced TH/TD, which could be due to the increased test construction efficiency by speeding up test resorption, increasing hole diameter and trabecula length, and decreasing trabecula width. It was recommended that the dietary protein level should be no more than 10 % for achieving rapid and coordinated growth of juvenile <em>S. intermedius</em>.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"40 ","pages":"Article 102578"},"PeriodicalIF":3.2,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143142669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of a Southern catfish (Silurus meridionalis) spermatogonial stem cell line capable of sperm production in vitro","authors":"Jianeng Li ,&nbsp;Changle Zhao ,&nbsp;Lei Liu ,&nbsp;Shuqing Zheng ,&nbsp;Deshou Wang ,&nbsp;Changwei Shao ,&nbsp;Jing Wei","doi":"10.1016/j.aqrep.2024.102587","DOIUrl":"10.1016/j.aqrep.2024.102587","url":null,"abstract":"<div><div>Southern catfish <em>(Silurus meridionalis</em>) is widely distributed in the Yangtze River and its tributaries and is valued for its rapid growth, nutritional content, pleasant taste, absence of intermuscular bone, and disease resistance. However, the species faces challenges in genetic breeding and germ-line preservation due to its late sexual maturity (2–3 years for males and 3–4 years for females) and single spawning cycle. In this study, we successfully established a spermatogonial stem cell line (designated as SC1) derived from the testis of a four-month-old Southern catfish. This cell line has exhibited continuous growth for more than one year, showing significant alkaline phosphatase activity, and maintaining a diploid karyotype. It exhibited expression of germ cell markers, including glial cell-derived neurotrophic factor family receptor alpha-1 (<em>gfrα1</em>) <em>a</em> and <em>b</em>, <em>pou5f3</em>, <em>nanog</em>, and <em>vasa</em>, but no expression of somatic cell markers such as <em>amh</em>, <em>wt1a</em>, and <em>wt1b</em>. Notably, SC1 has shown the ability to differentiate into sperm-like cells in vitro following treatment with retinoic acid (RA) and/or 11-ketotestosterone (KT). Transfection efficiency exceeding 50 % was achieved utilizing microporous membrane electroporation. Furthermore, the sustained proliferation of SC1 was found to depend on a combination of various factors, particularly the extract from Nile tilapia embryos. Collectively, our findings indicate the successful establishment of a Southern catfish spermatogonial stem cell line that exhibits sustained proliferation and sperm production in vitro. This study provides a foundation for generating genome-edited sperm for germ-line transmission in this species and offers a valuable tool for investigating fish spermatogenesis <em>in vitro</em>.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"40 ","pages":"Article 102587"},"PeriodicalIF":3.2,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143142667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Induction of larval settlement and metamorphosis of Mercenaria mercenaria using excess Ca2＋and Mg2+alone or in combination","authors":"Jian Liang ,&nbsp;Bajun Shi ,&nbsp;Yongren Li ,&nbsp;Shuang Liang ,&nbsp;Huiru Liu ,&nbsp;Wei Dai ,&nbsp;Yongjun Guo","doi":"10.1016/j.aqrep.2024.102588","DOIUrl":"10.1016/j.aqrep.2024.102588","url":null,"abstract":"<div><div>The induction effect of excess Ca²⁺ and Mg²⁺ alone or in combination on settlement ^{and metamorphosis of} <em>Mercenaria mercenaria</em> larvae were studied by initiating ion supplementation at two different developmental stages. ^Pediveligers were supplemented with a range of excess Ca²⁺ (0–18 mM), Mg²⁺ (0–40 mM) and Ca^2＋/ Mg²⁺ (0–7.5/0–37.5 mM), respectively. Results showed excess Ca²⁺ and Mg²⁺ alone or in combination could induce settlement and ^{metamorphosis of <em>M. mercenaria</em>. The o}ptimal concentrations of Ca²⁺, Mg²⁺, and Ca²⁺/Mg²⁺ were 10 mM, 20 mM and 3.125/15.625 mM, respectively. The supplementation of the above optimal concentration of ions from D-shaped larvae stage also significantly induced larval metamorphosis (<em>P</em> &lt; 0.05), and at the same concentration, the induction effect was better than that initiating from pediveliger stage. In addition, compared with the control group, ion supplementation from D-shaped larvae stage significantly increased the survival rate of pediveligers (<em>P</em> &lt; 0.05) and prolonged the planktonic duration. The longer the planktonic duration, the larger the larval size at the end of the planktonic stage and the higher ^{metamorphosis rate of} larvae. In the hatchery seed production of <em>M. mercenaria</em>, it is recommended to supplement 3.125/15.625 mM Ca^2＋/ Mg²⁺ from the D-shaped larvae stage to induce the settlement and metamorphosis.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"40 ","pages":"Article 102588"},"PeriodicalIF":3.2,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143142665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulation of glycolipid and amino acid metabolism in juvenile triploid crucian carp (Carassius auratus) by oral administration of aspartic acid","authors":"Yu Wang ,&nbsp;Bo Dong ,&nbsp;Liyun Wu ,&nbsp;Dong Han ,&nbsp;Xiaoming Zhu ,&nbsp;Haokun Liu ,&nbsp;Yunxia Yang ,&nbsp;Shouqi Xie ,&nbsp;Zhen Liu ,&nbsp;Junyan Jin","doi":"10.1016/j.aqrep.2024.102574","DOIUrl":"10.1016/j.aqrep.2024.102574","url":null,"abstract":"<div><div>Aspartic acid, a nonessential amino acid, plays crucial roles in protein synthesis, tricarboxylic acid cycle, and glycolipid metabolism in mammals. However, corresponding studies of the role of aspartic acid in fish remain limited. This study aimed to investigate the effects of aspartic acid on physiological metabolism of triploid crucian carp (<em>Carassius auratus</em>). The fish (48.2 ± 2.4 g) were administered with low (0.22 g/mL, LA) or high (0.44 g/mL, HA) concentrations of L-aspartic acid solution, while normal saline was given to the control group. The results demonstrated that plasma glucose levels in both the LA and HA groups were remarkably decreased at 3 h after administration, while hepatic glycogen content increased dramatically. Furthermore, compared to the LA group, the glucose levels in the HA group were noticeably higher at 6 h. Plasma free fatty acids were remarkably decreased in both the LA and HA groups compared to control group at 3 h after administration, whereas the HA group had considerably higher plasma total cholesterol levels than the LA group. Additionally, the gene expression levels of genes involved in synthesis of fatty acids (<em>acc</em>, <em>srebp1</em> and <em>fasn</em>) were significantly higher in the liver of the HA group compared with the LA group. However, aspartic acid did not affect the gene expression of key enzymes involved in hepatic fatty acid β-oxidation. Both the LA and HA groups had significantly higher plasma levels of free aspartic acid, glutamic acid, and total essential amino acids. Additionally, the gene expression level and enzymatic activity of hepatic aspartate aminotransferase were increased (<em>P</em> &lt; 0.05). In conclusion, different doses of aspartic acid induced distinct physiological and metabolic responses in triploid crucian carp. High-dose aspartic acid enhanced amino acid metabolism in the liver, thus increasing the capacity for <em>de novo</em> synthesis of fatty acids.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"40 ","pages":"Article 102574"},"PeriodicalIF":3.2,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143142668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}