Ferulic acid improved lipid metabolism of blunt snout bream, Megalobrama amblycephala juveniles via the intestinal microbiota and metabolites

IF 3.7 2区农林科学 Q1 FISHERIES

Aquaculture Reports Pub Date : 2025-09-02 DOI:10.1016/j.aqrep.2025.103059

Yan Lin , Xiangjun Leng , Xiaoqin Li , Linjie Qian , Siyue Lu , Qiaoqiao Mu , Zhengyan Gu , Wenqiang Jiang , Linghong Miao , Xianping Ge

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引用次数: 0

Abstract

Fish is an important source of protein for human, and lipid homeostasis is essential for fish health. As a green plant extract, ferulic acid (FA) plays a crucial role in modulating lipid metabolism. This study investigate the effects of dietary FA supplementation on lipid metabolic homeostasis, intestinal microbiota, and metabolites in plasma and intestinal contents of Megalobrama amblycephala juveniles. M. amblycephala (average weight: 9.75 ± 0.04 g) juveniles were fed with three feeds containing 0 (Control), 100 (FA100), and 200 (FA200) mg/kg FA with equivalent nitrogen and energy levels for 8 weeks. The results indicate that FA supplementation significantly reduce the content of plasma lipids and liver lipids, and markedly increase total bile acid content in liver. It is possible that FA regulates liver fatty acid metabolism and cholesterol-bile acid metabolism by up-regulating genes such as carnitine palmitoyl transferase 1 A, cholesterol 7α-hydroxylase, and down-regulating genes such as sterol regulatory element-binding protein. The diversity of the intestinal microbiota is significantly enhanced, while the abundance of harmful bacteria exhibits a marked reduction in FA100 group. Further, metabolites are altered in FA100 group. Epoxy eicosatrienoic acid is a co-upregulated metabolite in both intestinal contents and plasma, and was significantly correlated with multiple indicators related to lipid metabolism. In summary, supplementing FA to feed decrease liver and plasma lipid accumulation and maintained lipid metabolic homeostasis. This may be due to the changed intestinal microbiota of M. amblycephala, as well as enrichment of intestinal and plasma metabolites.

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阿魏酸通过肠道菌群和代谢物改善钝口鲷幼鱼的脂质代谢

鱼类是人体重要的蛋白质来源，脂质稳态对鱼类健康至关重要。阿魏酸作为一种绿色植物提取物，在调节脂质代谢中起着至关重要的作用。本试验旨在研究饲粮中添加FA对双头巨鲷幼鱼脂质代谢稳态、肠道微生物群、血浆和肠道内容物代谢物的影响。将平均体重为9.75 ± 0.04 g的双头鲂幼鱼饲喂含有0（对照）、100 （FA100）和200 (FA200) mg/kg FA的饲料，并按同等氮能水平饲喂8周。结果表明，添加FA可显著降低血浆脂质和肝脏脂质含量，显著提高肝脏总胆汁酸含量。FA可能通过上调肉毒碱棕榈酰转移酶1 A、胆固醇7α-羟化酶等基因和下调固醇调节元件结合蛋白等基因调控肝脏脂肪酸代谢和胆固醇-胆汁酸代谢。FA100组肠道菌群多样性显著增强，有害菌丰度显著降低。此外，FA100组代谢产物发生改变。环氧二碳三烯酸是肠道内容物和血浆中共同上调的代谢物，与脂质代谢相关的多个指标显著相关。综上所述，饲料中添加FA可减少肝脏和血浆脂质积累，维持脂质代谢稳态。这可能是由于羊头分枝杆菌肠道菌群的改变，以及肠道和血浆代谢物的富集。

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来源期刊

Aquaculture Reports Agricultural and Biological Sciences-Animal Science and Zoology

CiteScore

5.90

自引率

8.10%

发文量

469

审稿时长

77 days

期刊介绍： Aquaculture Reports will publish original research papers and reviews documenting outstanding science with a regional context and focus, answering the need for high quality information on novel species, systems and regions in emerging areas of aquaculture research and development, such as integrated multi-trophic aquaculture, urban aquaculture, ornamental, unfed aquaculture, offshore aquaculture and others. Papers having industry research as priority and encompassing product development research or current industry practice are encouraged.