Aquaculture Reports最新文献

{"title":"Methylation profile of the testes of the flatfish Solea senegalensis","authors":"Daniel Ramírez ,&nbsp;María Esther Rodríguez ,&nbsp;Robert Mukiibi ,&nbsp;Carolina Peñaloza ,&nbsp;Helena D’Cotta ,&nbsp;Diego Robledo ,&nbsp;Laureana Rebordinos","doi":"10.1016/j.aqrep.2024.102405","DOIUrl":"10.1016/j.aqrep.2024.102405","url":null,"abstract":"<div><div>In aquaculture production, fish phenotypes are influenced by a combination of external and internal factors, while the underlying mechanisms often involve distinct DNA methylation patterns. These play a fundamental role in regulating gene expression in response to environmental shifts. The Senegalese sole (<em>Solea senegalensis</em>) holds significant potential in aquaculture due to its high commercial importance. However, its production faces several challenges, including the inability of F1 soles to reproduce. In our study, we analysed the methylation patterns in the testicular DNA of 12 male <em>S. senegalensis</em> individuals from four distinct groups, which differed in rearing origins (wild and F1 soles) and sexual maturity stages (mature and immature). We employed reduced representation bisulfite sequencing to identify significant methylation differences among studied groups. The differential methylation analysis showed a substantial disparity between the F1 and wild groups. This difference was evident in both the number of different methylated CpGs (DMCpGs) and methylation levels, highlighting a distinction in groups reared under different conditions. The comparison between immature and mature wild groups revealed notable differences, suggesting that the testes methylation profile undergoes few changes in wild individuals during sexual maturity. These differences in methylation between wild and hatchery-born and reared offspring imply epigenetic modifications triggered by rearing in captivity and by the absence of natural <em>stimuli</em> such as temperature fluctuations. We further annotated the DMCpGs based on their position and co-localization with genes in the Senegalese sole genome. Our analysis revealed that the methylation differences observed in DMCpGs were more pronounced in intron regions, with higher methylation levels. Conversely, the methylation differences in exon and promoter areas were more subtle. Upon annotation, we discovered that these DMCpG sites were located near or within various transcription factors. Our comprehensive analysis sheds light on the intricate methylation profile of <em>S. senegalensis</em>, focusing on genes associated with reproductive traits, particularly those related to sex determination (SD) systems and spermatogenesis. Overall, we observe a trend toward higher methylation levels in DMCpGs when comparing F1 individuals to wild groups, as well as immature to mature wild groups. Hence, sexual maturity and place of origin resulted in marked methylation differences, which could potentially affect the expressions of genes involved in both development and reproduction of farmed males. This analysis explores the gene-regulating effects of this epigenetic mechanism providing valuable insights into the variations in methylation found in the testes of Senegalese sole.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102405"},"PeriodicalIF":3.2,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142433248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"External nutrients fattening promotes hepatopancreases development, metabolism, nutrient deposition and flavor yield during the maturation stage in female Chinese mitten crab (Eriocheir sinensis)","authors":"Changyou Song ,&nbsp;Jianxiang Chen ,&nbsp;Jiyan He ,&nbsp;Miaomiao Xue ,&nbsp;Pao Xu ,&nbsp;Huiming Ding ,&nbsp;Ningyuan Wu ,&nbsp;Ruicheng Zhao ,&nbsp;Gangchun Xu ,&nbsp;Hongxia Li","doi":"10.1016/j.aqrep.2024.102428","DOIUrl":"10.1016/j.aqrep.2024.102428","url":null,"abstract":"<div><div>The nutritive value and maturity status of hepatopancreases dominates the quality of Chinese mitten crab, <em>Eriocheir sinensis</em>. However, the underlying function and regulatory mechanism, as well as the external nutrient factors on the development of hepatopancreases in different growth stages of E. sinensis remains unclear. In this study, we first revealed the function of hepatopancreases feeding with iced trash fish (ITF) during the whole culture period; and further investigated the underlying mechanism on nutrient deposition and flavor yield feeding with formulated feed (FEED) in replacement of ITF in female <em>E. sinensis</em>. From the results, growth performance and metabolism indicate <em>E. sinensis</em> was robustly developed, and ITF promotes growth and hepatopancreases development during the maturation period. Meanwhile, activation of fatty acid and amino acid metabolism in hepatopancreases resulted in depletion of monounsaturated fatty acids (MUFAs) but promoted protein deposition during the maturation stage. Integrated analysis of transcriptome and metabolome reveal metabolism, biosynthesis, and flavor presentation were intensively active during maturation, evidenced by the expression of key genes of <em>ALDH</em>, <em>TBXAs</em>, <em>HADHA</em>, <em>UPB1</em>, <em>CYP2</em>, <em>GST</em>, and <em>γ-GT-X1</em>; as well as the key metabolites of GMP and IMP. Additionally, substitution of ITF with FEED further confirm full price artificial feed could meet the nutrient requirements during the maturation period, evidenced by the growth performance, fatty acid and amino acid derived nutrient and flavor yield in hepatopancreases. In conclusion, nutrient and flavor deposition was associated with the fast development of hepatopancreases, and fatten with full price compound feed contributes to the nutrient requirement and flavor yield during the maturation stage.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102428"},"PeriodicalIF":3.2,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142437830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High soybean dietary supplementation with quercetin improves antioxidant capacity of spotted sea bass Lateolabrax maculatus","authors":"Longhui Liu ,&nbsp;Yanbo Zhao ,&nbsp;Zhangfan Huang ,&nbsp;Zhongying Long ,&nbsp;Huihui Qin ,&nbsp;Hao Lin ,&nbsp;Sishun Zhou ,&nbsp;Lumin Kong ,&nbsp;Jianrong Ma ,&nbsp;Yi Lin ,&nbsp;Zhongbao Li","doi":"10.1016/j.aqrep.2024.102429","DOIUrl":"10.1016/j.aqrep.2024.102429","url":null,"abstract":"<div><div>This study aimed to examine the effect of quercetin (QUE) on the antioxidant capacity of spotted sea bass that were fed a high soybean meal diet. 44 % fish meal was used as a healthy control, and soybean meal instead of 50 % fish meal induced a negative reaction. On this basis, 0.25, 0.50, 0.75, and 1.00 g/kg QUE were added, respectively. A total of 540 spotted sea bass were randomly allocated into 6 groups and provided with diets that corresponded to their respective groups for a duration of 56 days. The results showed a significant decrease in the antioxidant capacity of spotted sea bass fed a high soybean meal diet. The inclusion of QUE considerably improved the antioxidant capacity of spotted sea bass. This was evidenced by the notable rise in glutathione (GSH) levels in the intestine and liver, as well as the elevated levels of catalase (CAT) and total antioxidant capacity (T-AOC) in the liver. Furthermore, transcriptome results showed that the S-transferase (GST) gene in the glutathione metabolism pathway was significantly down-regulated by high soybean meal, while with the addition of QUE, noteworthy alterations in genes associated with the PI3K-Akt signaling pathway, Foxo signaling pathway, and AMPK signaling pathway. These signaling pathways are involved in signal transduction, apoptosis, and oxidative stress. On the other hand, genes related to energy metabolism, such as phosphoenolpyruvate carboxykinase (PEPCK), were observed to be upregulated. These pathways could potentially contribute to the antioxidant properties of QUE.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102429"},"PeriodicalIF":3.2,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142433246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of pale shrimp disease in Pacific white shrimp (Litopenaeus vannamei) caused by Photobacterium damselae subsp. damselae in low salinity culture conditions","authors":"Niti Chuchird ,&nbsp;Natnicha Chongprachavat ,&nbsp;Wiranya Suanploy ,&nbsp;Lalitphan Kitsanayanyong ,&nbsp;Putsucha Phansawat ,&nbsp;Arunothai Keetanon ,&nbsp;Parattagorn Wimanhaemin ,&nbsp;Tirawat Rairat","doi":"10.1016/j.aqrep.2024.102416","DOIUrl":"10.1016/j.aqrep.2024.102416","url":null,"abstract":"<div><div>Pale shrimp disease is a serious emerging disease affecting juvenile Pacific white shrimp (<em>Litopenaeus vannamei</em>) in the low salinity areas of Southern Thailand. The most prominent clinical sign is a uniformly pale body coloration, with a mortality rate of nearly 100 % in severe cases. This study aimed to identify the causative agents of this disease. The affected shrimp from the natural outbreak ponds were collected for disease investigation. The disease was proven to be transmissible, as feeding-challenged shrimp showed pale body characteristics. To demonstrate the bacterial etiology of the disease, bacteria were isolated from naturally diseased shrimp and identified using <em>16S rRNA</em> gene sequencing. Pure cultures were inoculated into healthy shrimp. Among the different bacterial isolates (mainly <em>Vibrio</em> spp.), only <em>Photobacterium damselae</em> subsp. <em>damselae</em> (PDD) induced clinical signs of a pale body. Thus, PDD was examined for pathogenicity <em>via</em> immersion (10⁵ CFU/mL) and oral gavage (10⁷ CFU/shrimp) at 10 ppt. On day 7, the challenged shrimp showed pale body coloration, and the survival rates of both groups were 53 % and 10 %, respectively, whereas no mortality was observed in the control shrimp. Histologically, the hepatopancreatic tubules of the PDD-infected shrimp were atrophic, and lymphoid organ spheroids, myopathy, and hemocytic infiltration in the muscle were also evident. These histopathological changes are generally consistent with those observed in natural outbreaks. Consequently, our findings revealed that the enigmatic pale shrimp disease is caused by PDD, a hitherto unknown pathogen that causes massive shrimp mortality.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102416"},"PeriodicalIF":3.2,"publicationDate":"2024-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142428798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adaptive artificial multiple intelligence fusion system (A-AMIFS) for enhanced disease detection in Nile Tilapia","authors":"Achara Jutagate ,&nbsp;Rapeepan Pitakaso ,&nbsp;Surajet Khonjun ,&nbsp;Thanatkij Srichok ,&nbsp;Chutchai Kaewta ,&nbsp;Peerawat Luesak ,&nbsp;Sarayut Gonwirat ,&nbsp;Prem Enkvetchakul ,&nbsp;Tuantong Jutagate","doi":"10.1016/j.aqrep.2024.102418","DOIUrl":"10.1016/j.aqrep.2024.102418","url":null,"abstract":"<div><div>The development of an efficient disease detection system for Nile Tilapia is critical due to the significant economic and food security impacts of disease outbreaks. Traditional disease identification methods are labor-intensive, inefficient, and often fail to detect early signs of disease, leading to substantial economic losses. This study introduces the Adaptive Artificial Multiple Intelligence Fusion System (A-AMIFS), an advanced model that innovatively combines image augmentation, ensemble image segmentation methods, and ensemble Convolutional Neural Network (CNN) architectures. The system utilizes a non-population-based artificial multiple intelligence system (np-AMIS) for optimizing image augmentation and a population-based system (Pop-AMIS) for decision fusion, demonstrating superior performance. Evaluated on two novel datasets, Nile Tilapia Disease-1 (NTD-1) and Nile Tilapia Disease-2 (NTD-2), the system achieved an overall accuracy of 98.26 %, precision of 98.35 %, recall of 98.30 %, and an F1-score of 98.32 %, significantly outperforming existing methodologies. Additionally, a \"chatbot\" feature was developed to enable farmers to automatically detect fish diseases using the ensemble model as the backend classification system, achieving an impressive classification accuracy of over 98 %. These results underscore the system's robustness in detecting various diseases in Nile Tilapia and its potential to transform disease detection in aquaculture. The proposed system reduces manual labor, optimizes disease identification processes, and enhances disease management strategies, promoting more sustainable and productive aquaculture practices. This research highlights the indispensable role of AI techniques in overcoming the complex challenges of disease detection and management in aquaculture, presenting efficient and effective disease management practices. By leveraging advanced image augmentation, ensemble segmentation methods, and ensemble CNN architectures, this study presents a revolutionary approach to disease detection in Nile Tilapia. The integration of a user-friendly chatbot interface further enhances the technology's accessibility and practical application, empowering farmers to proactively manage disease outbreaks and mitigate economic losses.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102418"},"PeriodicalIF":3.2,"publicationDate":"2024-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142428746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Embryonic and larval development of black jewfish, Protonibea diacanthus: A new candidate for aquaculture in northern Australia","authors":"Andrea L. Taylor ,&nbsp;Alexander J. Basford ,&nbsp;Duong N. Duong ,&nbsp;Joshua N.J. Herrits ,&nbsp;Matthew W.J. Osborne","doi":"10.1016/j.aqrep.2024.102422","DOIUrl":"10.1016/j.aqrep.2024.102422","url":null,"abstract":"<div><div>Black jewfish <em>Protonibea diacanthus</em> is a large, commercially important fish distributed across the tropical Indo-Pacific. In Australia, there is an established commercial wild fishery for the species but it’s aquaculture potential has not been investigated. This research presents hatchery production of native <em>P. diacanthus</em> in Darwin, Australia and documents ontogeny of early life stages of this species over three trial runs. Wild <em>P. diacanthus</em> were induced to spawn with luteinizing hormone-releasing hormone analog (LHRHa) and eggs were released approximately 25–28 h after injection (0.88 ± 0.01 mm in diameter with oil globule 0.24 ± 0.01 mm). Larvae hatched 12 h after spawning. At one day post hatch (dph) larvae were 2.68 ± 0.01 mm standard length with 0.15 ± 0.01 mm oil globule diameter. Larvae mouths opened at 31 h post hatch (hph) and first feeding was seen at 57 hph. Swim bladder inflation (92 %) occurred between 40 and 62 hph. Larvae were progressively fed rotifers, <em>Artemia</em> and Otohime microdiet, and metamorphosis occurred between 20 and 25 dph, completing as they reached over 13 mm standard length. Tail biting cannibalism began with the onset of metamorphosis and caused significant mortality until approximately 24 dph when metamorphosed juveniles could be handled and moved to low stocking density systems. This study demonstrates that northern Australian <em>P. diacanthus</em> is amenable to hatchery production and provides important foundational knowledge for this emerging aquaculture species.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102422"},"PeriodicalIF":3.2,"publicationDate":"2024-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142428796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of α-lipoic acid supplementation in a high-fat diet on growth, lipid metabolism and mitochondrial function in spotted seabass (Lateolabrax maculatus)","authors":"Shan-pei Gan ,&nbsp;Wen-wen Huang ,&nbsp;Xiao-jiang Mao ,&nbsp;Hui-juan Ren ,&nbsp;Francisco A. Guardiola ,&nbsp;Kang-le Lu ,&nbsp;Samad Rahimnejad","doi":"10.1016/j.aqrep.2024.102408","DOIUrl":"10.1016/j.aqrep.2024.102408","url":null,"abstract":"<div><div>The aim of this study was to explore the impacts of incorporating α-lipoic acid (LA) in a high-fat diet (HFD) on growth, lipid metabolism and mitochondrial function in <em>Lateolabrax maculatus.</em> A normal-fat diet (NFD, 10 % lipid), a high-fat diet (HFD, 16 % lipid) and three LA-added HFD (200, 400, or 600 mg kg⁻¹ LA) were prepared and fed to five group of <em>L. maculatus</em> for 57 days. Supplementation of 400 mg kg⁻¹ LA to the HFD improved growth performance and feed utilization. LA treatment reduced the lipids concentration in serum and liver. Moreover, application of 400 mg kg⁻¹ LA in HFD notably decreased the activity of transaminases in serum. Liver oil red O staining results revealed the larger area of red lipid droplets in HFD group compared to the NFD group. Also, abdominal fat HE staining results showed larger adipocytes in the HFD group. Interestingly, LA application led to a reduction in the area of red lipid droplets and a decrease in the size of adipocytes. Furthermore, LA addition alleviated the HFD-induced oxidative stress as identified by enhanced mitochondrial citrate synthase, isocitrate dehydrogenase, α-ketoglutarate dehydrogenase, and succinate dehydrogenase activities. Meanwhile, LA administration induced the activation of mitochondrial biogenesis, and up-regulated the expression of autophagy-related genes. In conclusion, LA incorporation into HFD for <em>L. maculatus</em> promoted growth performance, enhanced liver mitochondrial function, and mitigated excessive fat accumulation in the liver.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102408"},"PeriodicalIF":3.2,"publicationDate":"2024-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142428747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Short-term starvation and refeeding in the greater amberjack (Seriola dumerili, Risso 1810): New insights on physiological and metabolic traits","authors":"Anyell Caderno ,&nbsp;Paula Simó-Mirabet ,&nbsp;Miguel García-Zara,&nbsp;Juan Antonio Martos-Sitcha","doi":"10.1016/j.aqrep.2024.102403","DOIUrl":"10.1016/j.aqrep.2024.102403","url":null,"abstract":"<div><div>The greater amberjack (<em>Seriola dumerili</em>) is a fish species with high commercial value and excellent growth rates, which has led to increasing interest in the aquaculture sector to optimize and expand its production. To gain a deeper insight into its physiological aspects, the present study aimed to evaluate the metabolic responses of <em>S. dumerili</em> juveniles after a short period of starvation and subsequent refeeding. For this purpose, the fish were fed <em>ad libitum</em> twice a day for four weeks and were then subjected to a starvation challenge followed by a refeeding period of seven days each. After one week of starvation, the fish showed weight loss but were able to adapt their metabolism by maintaining carbohydrate levels and using lipids and proteins as the main energy sources. All this was supported by decreased glycogenolytic potential during starvation, indicated by lower GP activity, and an increase in gluconeogenic pathway, as evidenced by the observed activity of FBP, LDH, and transaminases (ALT and AST). Moreover, the refeeding period stimulated growth processes by activating metabolic pathways, resulting in higher feed efficiency rates. Correspondingly, the highest plasma cortisol levels were observed during the refeeding period, stimulating anticipatory activity after starvation and contributing to metabolic recovery. In conclusion, <em>S. dumerili</em> can efficiently regulate its internal environment during a short period of starvation, improving feed efficiency and growth parameters after refeeding and rapidly restoring the initial metabolic state. These results could represent promising insights to enhance the feeding program for this species.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102403"},"PeriodicalIF":3.2,"publicationDate":"2024-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142428840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of WSSV and ammonia nitrogen stress on the enzyme activity and transcriptome of Litopenaeus vannamei","authors":"Shan Gao ,&nbsp;Linxin Dai ,&nbsp;Yue Wang,&nbsp;Zuluan Liang,&nbsp;Yinhuan Zhou,&nbsp;Chengbo Sun","doi":"10.1016/j.aqrep.2024.102412","DOIUrl":"10.1016/j.aqrep.2024.102412","url":null,"abstract":"<div><div>White spot syndrome virus (WSSV) is an important pathogen in the aquaculture industry that poses a serious threat to the shrimp farming industry. In this study, <em>Litopenaeus vannamei</em> was selected as the research object to determine the critical concentration of ammonia nitrogen that triggered the outbreak of WSSV. Multiomics techniques were used to study the comprehensive reaction mechanism of <em>L. vannamei</em>, which is in a state of WSSV infection and transforms into acute infection under conditions of ammonia nitrogen stress, from physiological, biochemical, and transcriptomic perspectives. The toxicity of ammonia nitrogen and WSSV to <em>L. vannamei</em> was measured by the LC₅₀ test, and semilethal concentrations at 72 hpi, 96 hpi, 120 hpi, and 144 hpi were obtained. H&amp;E staining revealed pathological changes in the intestinal tract induced by WSSV infection, ammonia nitrogen stress, and combined ammonia nitrogen and WSSV stress in <em>L. vannamei</em>. KEGG pathway enrichment analysis of the DEGs revealed that activation of the PI3K-Akt-mTOR signaling pathway triggers metabolic reprogramming of cells, which is conducive to the WSSV-induced Warburg effect and proliferation. Blocking the PI3K-Akt signaling pathway could reduce the apoptosis of WSSV-infected cells, thereby inhibiting viral replication. Notably, ammonia nitrogen stress disrupted the balance between shrimp and WSSV latent infection, increased the susceptibility to WSSV infection, and aggravated the severity of infection. The obstruction of ammonia metabolism leads to an increase in ammonia nitrogen levels, aggravates the inflammatory response and oxidative stress of the host, causes intracellular oxidative damage and host tissue damage, weakens the immune function of the host, and cannot effectively resist WSSV infection, leading to the acceleration of WSSV replication and disease outbreaks.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102412"},"PeriodicalIF":3.2,"publicationDate":"2024-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142428841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of dietary lysine on growth, nutrient utilization, and intestinal health in triploid rainbow trout (Oncorhynchus mykiss) fed low fish meal diets","authors":"Shuze Zhang ,&nbsp;Chang’an Wang ,&nbsp;Siyuan Liu ,&nbsp;Yaling Wang ,&nbsp;Shaoxia Lu ,&nbsp;Shicheng Han ,&nbsp;Haibo Jiang ,&nbsp;Hongbai Liu ,&nbsp;Yuhong Yang","doi":"10.1016/j.aqrep.2024.102402","DOIUrl":"10.1016/j.aqrep.2024.102402","url":null,"abstract":"<div><div>Lysine is one of the essential amino acids for fish growth and development. This study investigated the effect of dietary lysine on growth performance, digestion, and intestinal health of triploid rainbow trout (<em>Oncorhynchus mykiss</em>) (initial body weight: 9.00 ± 0.33 g) fed a low fish meal diet (15 %). Five dietary lysine levels (3.66 %, 3.84 %, 4.19 %, 4.32 %, and 4.55 %) were evaluated for 56 days, with three replicates of 30 fish per level. The results showed that the weight gain rate (198.55 %), specific growth rate (1.95), and VSI were maximized when the dietary lysine level was 4.19 %. The 4.19 % lysine group exhibited minimum moisture (66.66 %) and maximum crude protein (15.47 %), with no significant differences in crude lipid and ash contents. Growth factors mTOR and 4EBP-1, on the other hand, had the highest expression in the 4.19 % group, while IGF-1 and S6K1 peaked in the 4.32 % group. The anti-inflammatory factors TGF-β, NF-κB, IL-4, and IL-10 peaked at different levels and then decreased with increasing lysine levels. The pro-inflammatory factor IL-1β, on the other hand, had the lowest expression at the 4.19 % level. Based on quadratic regression analysis of specific growth rate, the lysine requirement of triploid <em>O. mykiss</em> in low fish meal diets was 4.24 %.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102402"},"PeriodicalIF":3.2,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142428792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}