Aquaculture Reports最新文献

{"title":"Cholesterol and highly unsaturated fatty acids: Exploring their synergistic roles in growth and lipid metabolism of juvenile Chinese mitten crab (Eriocheir sinensis)","authors":"Qiuran Yu ,&nbsp;Song Wang ,&nbsp;Erchao Li ,&nbsp;Shubin Liu ,&nbsp;Zhijuan Jiang ,&nbsp;Xiaodan Wang ,&nbsp;Jian G. Qin ,&nbsp;Liqiao Chen","doi":"10.1016/j.aqrep.2025.103091","DOIUrl":"10.1016/j.aqrep.2025.103091","url":null,"abstract":"<div><div>This study investigated the interactive effects of dietary cholesterol and highly unsaturated fatty acids (HUFAs) on the growth performance, molting dynamics, lipid metabolism, and nutrient signaling pathways in juvenile Chinese mitten crab (<em>Eriocheir sinensis</em>). A 3 × 2 factorial experiment was conducted over 8 weeks, manipulating three cholesterol levels (0 %, 0.06 %, 0.6 %) and two HUFAs levels (0 %, 0.4 %, EPA:DHA 1:3). Results demonstrated a significant synergistic interaction between dietary cholesterol and HUFAs. The combination of 0.06 % cholesterol and 0.4 % HUFAs yielded superior growth outcomes, including the highest final body weight, weight gain, specific growth rate, and lowest feed conversion ratio, surpassing the effects of individual supplementation. This optimal dietary combination also significantly increased whole-body crude protein and lipid content. Analysis of serum biochemical parameters revealed that cholesterol increased triacylglycerides, high-density lipoprotein cholesterol, and total cholesterol, while HUFAs decreased low-density lipoprotein cholesterol. Tissue lipid content and fatty acid profiles varied across molting stages. The combined diet enhanced lipid deposition in the hepatopancreas during the premolt stage. It also promoted the efficient utilization of lipids postmolt, particularly for EPA and DHA. Molecular analyses revealed a synergistic effect of the combined cholesterol and HUFAs supplementation, especially during the postmolt stage. This combination significantly upregulated key genes involved in lipid synthesis (<em>srebp1</em>, <em>fas</em>) in the hepatopancreas and muscle. Furthermore, it enhanced the expression of genes related to lipid transport (<em>mttp</em>, <em>fatp</em>, <em>ldlr</em>). Furthermore, the combined treatment significantly upregulated the expression of growth factor signaling pathway genes (<em>mtorc1</em>, <em>s6k1</em>, <em>egfr</em>, <em>erk</em>) in both the hepatopancreas and muscle. This upregulation likely facilitated rapid cell growth and tissue repair. These findings highlight that optimal dietary cholesterol and HUFAs interact synergistically to enhance growth performance and modulate lipid metabolism and growth signaling throughout the molting cycle in <em>E. sinensis</em>.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"45 ","pages":"Article 103091"},"PeriodicalIF":3.7,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145027429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing genotype imputation pipeline for low-coverage whole genome sequencing data in spotted sea bass and its application in genomic prediction","authors":"Chong Zhang ,&nbsp;Yonghang Zhang ,&nbsp;Pengyu Li ,&nbsp;Cong Liu ,&nbsp;Lingyu Wang ,&nbsp;Yani Dong ,&nbsp;Donglei Sun ,&nbsp;Xin Qi ,&nbsp;Haishen Wen ,&nbsp;Kaiqiang Zhang ,&nbsp;Shaosen Yang ,&nbsp;Yun Li","doi":"10.1016/j.aqrep.2025.103088","DOIUrl":"10.1016/j.aqrep.2025.103088","url":null,"abstract":"<div><div>Genotype imputation following low-coverage whole genome sequencing (lcWGS) data offers a cost-effective approach for genotyping large populations, with significant potential to accelerate genomic selection in breeding programs. For spotted sea bass (<em>Lateolabrax maculatus</em>), genetic improvement is urgently required due to the degeneration of genetic traits and long generation intervals. However, the high costs associated with high-coverage WGS (hcWGS) for large populations have delayed breeding progress. To address this gap, the present study conducted a comprehensive evaluation of genotype imputation for lcWGS data down-sampled from 1107 individuals across four hcWGS datasets and aimed to develop an efficient imputation pipeline utilizing lcWGS data for spotted sea bass. Initially, 100data dataset was selected to preliminary assess the performance of various imputation pipelines. BEAGLE was excluded due to its lower accuracy and redundant computational requirements, while STITCH and GLIMPSE2 were retained for subsequent analyses. The effects of reference and target data on GLIMPSE2 imputation were then evaluated, identifying the optimal strategy for constructing the reference panel prioritizes population genetic diversity over sample size to maximizes imputation accuracy. It also highlighted the critical role of population structure, genetic relatedness and linkage disequilibrium (LD) level between reference and target data for imputation accuracy. Additionally, the imputation accuracy of STITCH and GLIMPSE2 was compared across three datasets, with GLIMPSE2 imputation using the optimal reference panel emerging as the most effective imputation pipeline for spotted sea bass. Finally, we demonstrated that lcWGS data combined with GLIMPSE2 imputation achieves predictive accuracy comparable to hcWGS data in genomic prediction. Our study presents an optimized workflow to impute lcWGS data in spotted sea bass and establishes the first publicly available reference panel with the highest known genetic diversity. This resource lays a crucial foundation for future genomic selection and breeding programs and serves as a valuable reference for genotype imputation in other aquaculture species.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"45 ","pages":"Article 103088"},"PeriodicalIF":3.7,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145027430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating suitable dietary lipid content for mandarin fish (Siniperca chuatsi), on the basis of growth, lipid metabolism, antioxidant capacity and flesh texture","authors":"Ye Gong ,&nbsp;Shengchao Liu ,&nbsp;Nihe Zhang ,&nbsp;Xuxiong Huang ,&nbsp;Zeqiang Sun ,&nbsp;Naisong Chen ,&nbsp;Songlin Li","doi":"10.1016/j.aqrep.2025.103076","DOIUrl":"10.1016/j.aqrep.2025.103076","url":null,"abstract":"<div><div>This study was designed to figure out suitable dietary lipid level for mandarin fish (<em>Siniperca chuatsi</em>). Five isonitrogenous diets with different lipid content (6 %, 9 %, 12 %, 15 %, and 18 %, named L6.72, L9.43, L12.09, L15.87, L18.04, respectively) were manufactured to feed the experimental mandarin fish (102.59 ± 0.07 g) twice daily. The five experimental groups each had three replicates, containing 30 mandarin fish. The feeding trail lasted for 56 days. The results revealed increasing dietary lipid content linearly increased the feed intake (FI), final body weight (FBW) and specific growth rate (SGR) of cultured fish. In addition, the crude lipid content in the whole body, liver and muscle of mandarin fish also significantly increased. The total superoxide dismutase (T - SOD) activity in the L12.09 group showed the highest value and the malondialdehyde (MDA) content in the L12.09 group was the lowest. The serum biochemical indexes revealed the increase of dietary lipid content linearly increased the serum total triglyceride (TG) content and aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities. The expression of genes relating to lipid synthesis in the liver and muscle, including <em>pparγ</em>, <em>fas</em>, <em>dgat1</em> and <em>acc1</em> showed a significant quadratic trend with increasing dietary lipid level, and the highest expression was found in the group of L12.09. The liver lipolysis genes, including <em>pparα</em>, <em>hsl</em> and <em>cpt1a</em> showed a quadratic trend with increasing dietary lipid levels, and the lowest expression occurred in the group of L12.09. However, the expression of these genes in the muscle significantly decreased with increasing dietary lipid levels. Additionally, dietary lipid content linearly decreased muscle hardness, adhesiveness and shear force and linearly increased muscle gumminess of cultured fish. In conclusion, dietary lipid levels affected the growth performance, lipid metabolism and flesh texture of cultured mandarin fish and suitable dietary lipid level was 12-15 %.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"45 ","pages":"Article 103076"},"PeriodicalIF":3.7,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145020534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soybean hull-based binders: Evaluation of growth and potential health benefits in Pacific white shrimp, Litopenaeus vannamei","authors":"Mediha Yildirim-Aksoy ,&nbsp;Burak Aksoy ,&nbsp;Rashida Eljack ,&nbsp;Benjamin H. Beck ,&nbsp;Carl D. Webster ,&nbsp;Matthew Paulson","doi":"10.1016/j.aqrep.2025.103096","DOIUrl":"10.1016/j.aqrep.2025.103096","url":null,"abstract":"<div><div>The water stability of aquafeeds, especially shrimp feed, is an important component of feed quality. Recently, our laboratory developed novel feed binders derived from soyhull to further improve water stability of the compound feeds. Here, a 10-week feeding study was conducted to evaluate the effects of three soyhull-based binders and three control binders (carboxymethyl cellulose, corn starch, and wheat gluten) incorporated in diets at 2 % on physiological (growth, body composition and plasma chemistry) and immunological (hemocyte count, hemocyanin, phenoloxidase activity) status of juvenile Pacific white shrimp, <em>Litopenaeus vannamei</em> (mean weight of 4.35 ± 0.10 g). During the growth trial, the physiological status of shrimp, measured by growth, survival, body composition and plasma chemistry (alkaline phosphatase, alanine aminotransferase, calcium, potassium, total protein, cholesterol), were not significantly affected by the dietary treatments. However, total hemocyte count and hemocyte lysate phenoloxidase activity were significantly higher in shrimp fed a diet with SH #3 binder than that of shrimp fed pellets with corn starch. No significant differences in immune status were observed among shrimp fed other diets. Based on weight gain, percent survival, and body composition, soyhull-based binders can be used successfully to replace wheat gluten, corn starch or carboxymethyl cellulose in shrimp diets. Additionally, the use of SH #3 binder may prove beneficial by improving the immune status of <em>L. vannamei</em>.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"45 ","pages":"Article 103096"},"PeriodicalIF":3.7,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145020532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of host-microbiota interactions in sea urchin (Strongylocentrotus intermedius) with “black mouth disease”","authors":"Anzheng Liu ,&nbsp;Dongsheng Chen ,&nbsp;Yeqing Zhou ,&nbsp;Tengyu Xing ,&nbsp;Yuchen Chen ,&nbsp;Xinyao Chen ,&nbsp;Jun Ding ,&nbsp;Luo Wang","doi":"10.1016/j.aqrep.2025.103084","DOIUrl":"10.1016/j.aqrep.2025.103084","url":null,"abstract":"<div><div>In recent years, the frequent outbreaks of black mouth disease have severely impacted the development and survival of sea urchin. Nevertheless, the mechanisms underlying host-microbiota interactions remains unclear. This study analyzed the structural and functional characteristics of the microbial community and investigated the host transcriptional patterns within the mouthparts of diseased sea urchins (<em>Strongylocentrotus intermedius</em>). The results showed that black mouth disease significantly affected the structure of the mouthparts microbiota in the <em>S. intermedius</em>. The dominant bacterial genera in the disease group are represented by <em>Vibrio</em>, <em>Photobacterium</em>, and <em>Arcobacter</em>. A total of 4645 differentially expressed genes were identified in the mouthpart of diseased <em>S. intermedius</em>, of which 1753 genes were upregulated and 2892 genes were downregulated. Multi-omics analysis revealed that infections by potential pathogens bacteria associated with black mouth disease, such as <em>Vibrio</em>, <em>Arcobacter</em>, <em>Fusibacter</em>, and <em>Photobacterium</em>, are associated with the upregulation of genes in host, including <em>HSP70IV</em>, <em>ATF5</em>, <em>NFkB1</em>, <em>Jun</em>, <em>CTSL</em> and <em>C3</em>. This upregulation enhances functions related to the complement system, transcription factors, and the mitochondrial unfolded protein response, potentially contributing to enhanced immune responses. The research has certain reference significance for the study of pathogenic host-microbiota interactions in aquatic organisms, and provides a theoretical basis for the prevention and control of sea urchin diseases.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"45 ","pages":"Article 103084"},"PeriodicalIF":3.7,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145011098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of butyric acid on the inhibition of Vibrio parahaemolyticus and its impacts on growth performance, intestinal microbiota, and water microbiota in Litopenaeus vannamei","authors":"Minghan Yin ,&nbsp;Zhangyi Zheng ,&nbsp;Ruiyan Xing ,&nbsp;Lefei Jiao ,&nbsp;Moussa Gouife ,&nbsp;Tinghong Ming ,&nbsp;Fei Kong ,&nbsp;Shuang Qiu ,&nbsp;Jiajie Xu","doi":"10.1016/j.aqrep.2025.103078","DOIUrl":"10.1016/j.aqrep.2025.103078","url":null,"abstract":"<div><div>Pathogenic <em>Vibrio</em> species have long posed a major challenge in shrimp aquaculture, while traditional antibiotic treatments are associated with numerous limitations, creating an urgent demand for novel preventive and therapeutic approaches. The present study employed <em>in vivo</em> and <em>in vitro</em> experiments to comprehensively evaluate the feasibility of butyric acid supplementation in <em>Litopenaeus vannamei</em> culture. <em>In vitro</em> antimicrobial assays demonstrated that under acidic conditions (pH 6), the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of butyric acid against <em>V. parahaemolyticus</em> were 20 mM and 40 mM, respectively. In contrast, under neutral conditions (pH 7), the MIC and MBC increased to 320 mM and 640 mM. At both MIC and MBC concentrations, butyric acid completely suppressed bacterial growth and significantly disrupted biofilm formation and cellular integrity. <em>In vivo</em> shrimp feeding trials revealed that dietary supplementation with 0.5 and 2.0 g/kg butyric acid significantly enhanced percent weight gain (PWG) and specific growth rate (SGR) (<em>P</em> &lt; 0.05). 0.5 g/kg butyric acid supplementation could reduce the feed conversion ratio (<em>P</em> &lt; 0.05). The addition of sodium butyrate (in its sodium salt form) significantly reduced the abundance of intestinal <em>Vibrio,</em> decreasing from 17.45 in the CK group to 8.63 %(SB0.5), 5.54 %(SB2.0), and 0.62 %(SB5.0) in the respective treatment groups, while promoting the proliferation of beneficial bacteria, particularly <em>Verrucomicrobiota</em> (<em>P</em> &lt; 0.05). Additionally, butyric acid supplementation significantly decreased the relative abundance of <em>Candidatus Aquiluna</em> and <em>Tenacibaculum</em> in aquaculture water (<em>P</em> &lt; 0.05). KEGG pathway of water microbiota showed that dietary supplementation with 0.5 and 2.0 g/kg sodium butyrate markedly exhibited significant enrichment in prodigiosin biosynthesis, while 5.0 g/kg butyric acid supplementation showed a marked reduction in the <em>Thiamine metabolism</em> pathway, which is critical for energy metabolism (<em>P</em> &lt; 0.05). This study not only offered a novel feed additive option for healthy shrimp aquaculture but also established a theoretical foundation for its application.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"45 ","pages":"Article 103078"},"PeriodicalIF":3.7,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145011097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of rapeseed meal replacing fishmeal on growth, body composition, amino acid digestion and transport, lipid metabolism, and immunity of black carp (Mylopharyngodon piceus)","authors":"Weihua Gao ,&nbsp;Kai Ming ,&nbsp;Yuanqi Fu ,&nbsp;Qiaoqing Xu ,&nbsp;Tilin Yi ,&nbsp;Yingbing Su ,&nbsp;Kai Luo ,&nbsp;Chenglong Wu ,&nbsp;Chengmin Zhao","doi":"10.1016/j.aqrep.2025.103079","DOIUrl":"10.1016/j.aqrep.2025.103079","url":null,"abstract":"<div><div>To assess the feasibility of replacing dietary fishmeal (FM) with rapeseed meal (RSM), a 120-day feeding trial was conducted on juvenile black carp (<em>Mylopharyngodon piceus</em>) with an initial body weight of 5.49 ± 0.02 g. Six isonitrogenous and isolipidic diets were formulated by substituting FM with 0 %, 10 %, 20 %, 30 %, 40 %, and 50 % RSM. The results showed that the 20 % replacement level did not significantly affect growth performance, feed conversion ratio, protease activity, muscle amino acid composition, and crude protein digestibility in black carp. The expressions of amino acid transporter-related genes in the intestine and muscle were downregulated with a significant linear pattern, but none of them showed significant differences at the 20 % replacement level (except for <em>slc6a19</em> in the muscle). In the 40 % and 50 % replacement levels, the aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities in the serum, and the triglycerides (TG) content in the liver were significantly increased. Dietary RSM promoted lipid synthesis and reduced lipolysis, leading to abnormal lipid deposition, which impaired the health of the liver. In the serum and intestine, excessive dietary RSM reduced the activity of alkaline phosphatase (AKP), acid phosphatase (ACP), lysozyme (LZM), catalase (CAT), and superoxide dismutase (SOD) activity, and downregulated the expression of antioxidant- and immune-related genes, which led to reduced immunity in black carp. In summary, excessive FM replacement by RSM can significantly negatively affect growth, nutrient metabolism, and immunity in black carp. Based on growth performance and feed utilization, RSM can replace up to 20 % of FM in the black carp diet.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"45 ","pages":"Article 103079"},"PeriodicalIF":3.7,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145011096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of biosecurity implementation and use of aqua drugs in fish farming in Cameroon","authors":"Mohamed Moustapha Fokom Ndebé ,&nbsp;Frédéric Moffo ,&nbsp;Mohamed Moctar Mouliom Mouiche ,&nbsp;Mildred Naku Tangu ,&nbsp;Jenny Maïva Yango Tchoumbougnang ,&nbsp;Cyrille Talla Ngueko ,&nbsp;Julius Awah-Ndukum","doi":"10.1016/j.aqrep.2025.103068","DOIUrl":"10.1016/j.aqrep.2025.103068","url":null,"abstract":"<div><div>The production and intensification of fish is among the fastest growing sectors worldwide. In low- and middle-income countries, fish farming plays major roles in the self-sufficiency and socioeconomic livelihood of many households, with fish being the most consumed source of protein. However, outbreaks of diseases caused by various factors, such as a lack of appropriate hygiene and sanitary measures, pose a serious threat to the production of fish and hence disastrous economic consequences. Therefore, this study was carried out to assess biosecurity measures and drug use in fish farms in Cameroon. Surveys using semistructured questionnaires and on-farm observations were used to obtain information on the sociodemographic characteristics of the farmers, the biosecurity and preventive measures and the aqua drugs used on the fish farms in five regions (Adamawa, Centre, Littoral, South and West) of Cameroon. A total of 364 (94.79 %) fish farmers out of the 384 randomly selected from a list of farmers in the study regions were included in the final analysis. A majority of the surveyed farms (62.91 %) were in an enclosed environment, and 77.47 % had a footbath at the entrance of the farms. Nearly a quarter of the farmers used sanitary evacuation, and the quarantine of new fish was seen in 26.37 % of the farms. In addition, the study revealed a mean biosecurity score of 0.41 ± 0.16, with better scores recorded in intensive systems (0.50 ± 0.19) than in extensive systems. Age, level of education and farmer training in fish farming significantly influenced the implementation of biosecurity measures in the study area. Sodium hypochlorite (25.69 %) and tetracycline (63.74 %) are mostly used in fish farms as disinfectants and antibiotics, respectively, for the prevention and control of disease. Overall, farms with high and medium biosecurity scores frequently used aqua drugs (OR=13.93 and OR=7.36, respectively) more than farms with low biosecurity scores did. This study highlights the importance of biosecurity educational and awareness programs for improving fish productivity among fish farmers. Hence, this study suggests the use of alternative drugs, other than antimicrobials, to avoid the potential emergence and distribution of resistant bacteria with possible public health risks.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"45 ","pages":"Article 103068"},"PeriodicalIF":3.7,"publicationDate":"2025-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145007580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research on a precise feeding method for fry in recirculating aquaculture systems","authors":"Haihui Yang ,&nbsp;Xiaochan Wang ,&nbsp;Yinyan Shi ,&nbsp;Jihao Wang ,&nbsp;Bo Jia ,&nbsp;Chengquan Zhou ,&nbsp;Hongbao Ye","doi":"10.1016/j.aqrep.2025.103083","DOIUrl":"10.1016/j.aqrep.2025.103083","url":null,"abstract":"<div><div>Fry feeding in recirculating aquaculture systems (RAS) has gained prominence following China’s ban on fishing in the Yangtze River. Although previous researches have focused on dynamic adjustments to adult fish feeding status, research on fry feeding has been subsequently neglected. To fill this research gap, a precise fry feeding method was developed, comprising four main components: a fry feeding status detection module, a feeding control module, a precise feed discharging module, and a variable feed distribution module. The detection module utilizes the improved FFD-YOLO network which incorporates GhostNet, BiFPN and CA attention to detect fry feeding status, and real-time feeding decisions were made accordingly. Numerical simulations using Python were conducted to calculate the optimal feed coverage ratio, and Fuzzy-PID control was employed to rapidly adjust the rotational speed of the spreading disc. The experiments demonstrate that the FFD-YOLO algorithm achieved a precision of 91.33 %, a recall rate of 74.15 %, and a mAP_0.5 of 85.06 %, with a detection speed of 75 frames per second (FPS). Feeding distribution coverage ratios of 40 % and 80 % were recommended based on simulation results. The experimental results demonstrated that when feeding based on clear images, the errors of discharge and distribution were less than 10.2 % and 12.6 %, respectively. In contrast, when feeding based on blurred images, the errors exceeded 18.4 % and 24.1 %, respectively. Control experiments demonstrated that the proposed method can promote the growth of fry. This study provides a significant reference for future research on automatic fry feeding in industrial RAS.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"45 ","pages":"Article 103083"},"PeriodicalIF":3.7,"publicationDate":"2025-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145007835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial effects of chebulagic acid and chebulinic acid isolated from Terminalia chebula against Vibrio parahaemolyticus","authors":"Lu-Yao Wang ,&nbsp;Shi-Lu Huang ,&nbsp;Yu-Wei Zhang ,&nbsp;Xiao-Xin Huang ,&nbsp;Fen-Tao Sun ,&nbsp;Yao-Wu Fu ,&nbsp;Qi-Zhong Zhang","doi":"10.1016/j.aqrep.2025.103085","DOIUrl":"10.1016/j.aqrep.2025.103085","url":null,"abstract":"<div><div><em>Vibrio parahaemolyticus</em> (VP) is a common pathogenic bacterium in marine animals, causing significant economic losses to the aquaculture industry annually. There is an urgent need to develop environmentally friendly, safe and effective antibacterial agents. In this study, two active compounds were isolated and purified from the ethanol extract of <em>Terminalia chebula</em> and identified as chebulagic acid (CAA) and chebulinic acid (CIA) using mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy. The antibacterial results showed that both CAA and CIA exhibited a minimum inhibitory concentration (MIC) of 25 mg/L and a minimum bactericidal concentration (MBC) of 1250 mg/L against VP. The two compounds significantly increased the extracellular levels of AKP, nucleic acids, soluble proteins, and glucose of VP. Scanning electron microscopy results indicated that both active compounds significantly altered the cell morphology of VP, causing cell shrinkage and deformtion. Additionally, the two compounds exhibited potent inhibitory effects against several other pathogenic bacteria, including <em>Pseudomonas aeruginosa</em>, <em>Aeromonas versonii, A. hydrophila</em>, <em>V. alginolyticus</em>, and <em>V. vulnificus</em>. The 96-h half-lethal concentrations of CAA and CIA on <em>Ctenogobius giurinus</em> were 561.653 mg/L and 525.011 mg/L respectively. These values were 22.5 and 21.0 times higher than their MIC against VP, respectively. Therefore, CAA and CIA, derived from <em>T. chebula</em> extract, represent promising candidate molecules for the prevention and control of vibriosis.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"45 ","pages":"Article 103085"},"PeriodicalIF":3.7,"publicationDate":"2025-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145007579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}