Aquaculture Reports最新文献

{"title":"Effects of branched-chain amino acids on starvation-induced macroautophagy and chaperone-mediated autophagy in common carp (Cyprinus carpio)","authors":"Xianglin Cao ,&nbsp;Yi Guo ,&nbsp;Xue Shao ,&nbsp;Xinyu Ji ,&nbsp;Lulu Li ,&nbsp;Jiameng Zhou ,&nbsp;Jianjun Chen","doi":"10.1016/j.aqrep.2025.102932","DOIUrl":"10.1016/j.aqrep.2025.102932","url":null,"abstract":"<div><div>Most fish experience starvation stress during overwintering, typically resulting in excessive protein degradation and muscle atrophy. Branched-chain amino acids (BCAAs), are important substrates for protein synthesis and play active roles in promoting muscle growth. Although BCAAs have received widespread attention for their role in autophagy regulation, their ability to alleviate starvation-induced damage via macroautophagy and chaperone-mediated autophagy (CMA) pathways remains unclear. In this study, common carp were starved for 7 or 14 days, then administered BCAAs alone via gavage at 1.25 g/kg for 14 days. Starvation induced muscle atrophy and autophagy-related structural changes in carp muscle. Supplementation with BCAAs helped restore normal tissue morphology. Macroautophagy-related (<em>atg4b</em>, <em>atg12</em>, and <em>lc3</em>) and CMA-related (<em>hsp70</em> and <em>lamp2a</em>) gene expression was upregulated under starvation conditions. These genes were downregulated after supplementation with BCAAs, whereas <em>p62</em> and <em>gfap</em> showed the opposite trend. At the protein level, LC3-II, lysosome-associated membrane protein 2A (LAMP2A), and heat shock protein 70 (HSP70) were upregulated after starvation and downregulated after supplementation with BCAAs, but P62 showed the opposite trend. In addition, branched-chain amino acid transaminase (BCAT) and branched-chain ketoacid dehydrogenase (BCKDH) enzyme activity assays revealed that supplementation with BCAAs significantly increased amino acid metabolism. Overall, supplementation with BCAAs reduced macroautophagy and CMA levels in carp muscle tissues during starvation stress, and enhanced metabolic utilization of BCAAs. This study provides valuable insights for developing nutritional strategies to reduce starvation-induced muscle atrophy. These findings may help improve fish health and flesh quality during overwintering or feed restriction in aquaculture.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"43 ","pages":"Article 102932"},"PeriodicalIF":3.2,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144321273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Different growth responses to feed ration levels in two freshwater turtle juveniles, the Chinese three-keeled pond turtle Mauremys reevesii and the red-eared slider Trachemys scripta elegans","authors":"Changyi Zhang,&nbsp;Shufen Jiang,&nbsp;Xinyue Liu,&nbsp;Siting Zhong,&nbsp;Wenyi Zhang","doi":"10.1016/j.aqrep.2025.102931","DOIUrl":"10.1016/j.aqrep.2025.102931","url":null,"abstract":"<div><div>Turtle aquaculture is a rapidly expanding industry whereas the knowledge about the feed ration-dependent growth performances of turtles is limited. The present study investigated growth performance, physiological changes, and alterations in gut microbiota at gradient daily feed rations in two turtle juveniles, the Chinese three-keeled pond turtle <em>Mauremys reevesii</em> and the red-eared slider <em>Trachemys scripta elegans</em>. During 14 days of feeding, the feed rations exceeding 1 % body mass induced the rapid growth of both two turtles. The growth rates further increased at the feed ration of 2.5 % body mass in <em>T. scripta elegans</em>, but not in <em>M. reevesii</em>. Increased feed rations enhanced glucose metabolism in the liver of <em>T. scripta elegans</em> and the muscle of <em>M. reevesii</em>, respectively. Fatty acid metabolism was activated in both two turtles and protein metabolism was enhanced in <em>M. reevesii</em>. Accumulated triglyceride or protein was observed in the liver of <em>T. scripta elegans</em> and the muscle of <em>M. reevesii</em>. The regulation of succinate dehydrogenase and insulin-like growth factor 2 may relate to these processes. Raised feed rations changed redox homeostasis and decreased immune function in <em>M. reevesii</em>. High feed rations increased gut pathogens in <em>M. reevesii</em> whereas increased the probiotic <em>Lactobacillus</em> in the gut of <em>T. scripta elegans</em>. These interspecific differences may contribute to the different growth performances between these two turtle species under high food availability. Our results provide information for feed management and optimizing feeding efficiency in turtle aquaculture, and can guide the potential strategies to improve the growth performance of freshwater turtles.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"43 ","pages":"Article 102931"},"PeriodicalIF":3.2,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144314150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maternal supplementation with selenium nanoparticles enhances reproductive outcomes and larval quality in Asian seabass (Lates calcarifer) by influencing gene expression and physiological parameters","authors":"Seyed Ali Khorasaninasab ,&nbsp;Saeed Keyvanshokooh ,&nbsp;Mansour Torfi Mozanzadeh ,&nbsp;Ahmad Ghasemi ,&nbsp;Mahdi Naderi ,&nbsp;Samira Nazemroaya","doi":"10.1016/j.aqrep.2025.102933","DOIUrl":"10.1016/j.aqrep.2025.102933","url":null,"abstract":"<div><div>Selenium plays vital roles in antioxidant defense, immune function, and reproductive physiology in vertebrates. This study evaluated the impacts of dietary selenium nanoparticles (SeNPs) on reproductive outcomes, steroidogenesis, vitellogenesis, and larval quality in female Asian seabass (<em>Lates calcarifer</em>), by influencing gene expression, antioxidant capacity, hormone levels, and reproductive indices. Female broodfish (n = 12; 1990 ± 120 g) were fed either a basal diet (control) or a basal diet supplemented with 2 mg SeNPs/kg (SeNPs-2) for 120 days before spawning. Supplementation with SeNPs significantly (<em>P</em> &lt; 0.05) increased Se deposition in the liver, ovary, and larvae, enhancing antioxidant capacity (glutathione peroxidase and reduced-glutathione), and reducing oxidative stress marker (malondialdehyde; MDA) in both broodfish and offspring. The expression levels of genes involved in steroidogenesis (<em>star</em>, <em>P450scc</em>, and <em>3β-hsd</em>) and vitellogenesis (<em>zp2 and vtg</em>) were upregulated in the ovary or liver of the SeNPs-2 group (<em>P</em> &lt; 0.05). Despite comparable serum testosterone and estradiol levels (<em>P</em> &gt; 0.05), broodfish fed the SeNPs-2 diet exhibited significantly (<em>P</em> &lt; 0.05) lower progesterone levels, and lower serum cholesterol and triglyceride levels. Reproductive performance, as indicated by spawning frequency, relative fecundity, fertilization rate, and hatching rate, was significantly (<em>P</em> &lt; 0.05) improved in the SeNPs-2 group. Larvae descending from the SeNPs-fed broodfish exhibited higher expression of growth-promoting genes (<em>GH</em> and <em>IGF-I</em>), larger body size, and better developmental traits than those from the control group. These findings suggest that dietary SeNP supplementation positively influences Se bioavailability, gene expression, reproductive efficiency, and larval quality in <em>L. calcarifer</em>. SeNPs represent a promising nutritional strategy for optimizing broodstock performance and enhancing offspring quality in aquaculture.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"43 ","pages":"Article 102933"},"PeriodicalIF":3.2,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144306444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Using orthogonal array design (L9) for optimization of selected dietary supplements (vitamin C, astaxanthin, and soybean lecithin) in Caspian trout (Salmo caspius) broodstocks","authors":"Faraz Panjvini ,&nbsp;Kourosh Sarvi Moghanlou ,&nbsp;Raheleh Tahmasebi ,&nbsp;Ahmad Imani","doi":"10.1016/j.aqrep.2025.102935","DOIUrl":"10.1016/j.aqrep.2025.102935","url":null,"abstract":"<div><div>The present study was designed to evaluate the effects of different dietary supplements on the reproductive and physiological performance of Caspian trout (<em>Salmo caspius</em>) using an orthogonal array design (L9: 3³). For this purpose, 72 broodstock (with an average body weight of 2.5 kg) were fed nine experimental diets containing three levels of vitamin C (VC); 0, 300 and 700 mg/kg diet, astaxanthin (AX); 0, 50 and 100 mg/kg diet and soybean lecithin (SBL); 0, 6 and 9 % for 16 weeks prior to spawning. Higher absolute and relative fecundity, and fertilization rates were observed in fish fed diets containing AX ₍₁₀₀₎ + SBL _{(%6 and %9)} and VC ₍₇₀₀₎ + SBL _{(%6 and %9)}, respectively, compared to the control group (<em>p</em> &lt; 0.05). The fatty acid, VC and AX contents of fish eggs were also significantly affected by the dietary supplements (<em>p</em> &lt; 0.05). Innate immunity indices (serum lysozyme activity, alternative complement, and total immunoglobulin content) showed significant differences (<em>p</em> &lt; 0.05) among the experimental groups and their highest levels were observed in fish fed with VC ₍₃₀₀₎ + SBL _(%9) supplemented diet. In addition, fish fed the diets containing AX ₍₁₀₀₎ + SBL _(%9) and VC ₍₇₀₀₎ + SBL _(%6) revealed higher serum antioxidant enzyme activity (superoxide dismutase, catalase, and glutathione peroxidase) compared to other experimental groups (<em>p</em> &lt; 0.05). In conclusion, VC _300/700 + AX _{100 +} SBL _(9 %) was an effective combination of dietary supplements for improving the reproductive and physiological responses of Caspian trout.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"43 ","pages":"Article 102935"},"PeriodicalIF":3.2,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144306370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aquaculture performance of artificial interspecific backcross groups between the hybrid of Crassostrea hongkongensis × C. ariakensis and the two parental species","authors":"Yanping Qin ,&nbsp;Qinggan Xing ,&nbsp;Xingyou Li ,&nbsp;Jiangwei Li ,&nbsp;Weitao Wan ,&nbsp;Chao Yue ,&nbsp;Haitao Ma ,&nbsp;Jun Li ,&nbsp;Ying Pan ,&nbsp;Ziniu Yu ,&nbsp;Yuehuan Zhang","doi":"10.1016/j.aqrep.2025.102926","DOIUrl":"10.1016/j.aqrep.2025.102926","url":null,"abstract":"<div><div><em>Crassostrea hongkongensis</em> is popular in southern China for its white flesh color, unique flavor, and delicious taste. However, the disadvantages of slow growth, poor tolerance, and high mortality limit its production and market sales. The application of genetic breeding techniques to breed new breeding lines with fast growth and low mortality is an important approach to solve these industrial challenges, including interspecific backcross breeding. In this study, we established eight experimental groups (HH-<em>C. hongkonggensis</em> ♀ × <em>C. hongkonggensis</em> ♂; HA-<em>C. hongkonggensis</em> ♀ × <em>C. ariakensis</em>♂; AA-<em>C. ariakensis</em> ♂ × <em>C. ariakensis</em>; A/HA-<em>C. ariakensis</em>♀ × HA ♂; HA/HA-HA ♀ × HA ♂; H/HA-<em>C. hongkonggensis</em> ♀ × HA ♂; HA/H- HA ♀ × <em>C. hongkonggensis</em>♂ and HA/A-HA ♀ × <em>C. ariakensis</em> ♂), tracked and compared their phenotypic traits. The results confirmed that the hatching parameters of the interspecific backcross groups (HA/H, HA/A, H/HA and A/HA) were significantly lower than those of the pure parental progeny groups (HH and AA). Although HA/A did not always show a significant growth advantage in the larval stage, it exhibited the largest shell height during the marine culture period (except on the 210th day), and showed a significant shell height advantage on the 360th day (<em>P</em> &lt; 0.05). Moreover, there were obvious backcross advantages during the marine culture period, with backcross advantages (BAs) in shell height of 7.30 %, 17.66 %, 16.44 % and 10.95 % from the 90th to the 360th days, respectively. In addition, HA/A consistently ranked in the top three in terms of the incremental survival rate, being significantly higher than the grandparental offsprings (HH and AA), with HA/A showing the highest survival rates on the 210th and 360th days. Similarly, backcross advantages were observed at all time points, with BAs of 3.42 %, 5.96 %, 6.29 % and 0.43 %, respectively. Gonad development statistics and paraffin section results showed that all backcross groups were fertile and produced many normal gametes, with little difference in the ratios of males to females among the eight groups. In summary, the HA/A group showed significant backcross advantages in both growth and survival, with the best overall performance. This makes it a promising new strain that could help address the industrial problems of <em>C. hongkongensis</em> and has the potential for large-scale application.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"43 ","pages":"Article 102926"},"PeriodicalIF":3.2,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144297845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Culture performance and financial business of leopard coral (Plectropomus leopardus), humpback (Cromileptes altivelis), and brown-marbled (Epinephelus fuscoguttatus) grouper nursery","authors":"Belinda Astari ,&nbsp;Darmawan Setia Budi ,&nbsp;Suko Ismi ,&nbsp;Irzal Effendi ,&nbsp;Tatag Budiardi ,&nbsp;Iis Diatin ,&nbsp;Apriana Vinasyiam ,&nbsp;Ketut Sugama","doi":"10.1016/j.aqrep.2025.102923","DOIUrl":"10.1016/j.aqrep.2025.102923","url":null,"abstract":"<div><div>The nursery phase is critical in grouper aquaculture, influencing survival rates, growth performance, and economic feasibility. This study evaluates the culture performance and financial viability of three economically valuable grouper species: Leopard coral grouper (<em>Plectropomus leopardus</em>), humpback grouper (<em>Cromileptes altivelis</em>), and brown-marbled grouper (<em>Epinephelus fuscoguttatus</em>) over a 90-day nursery period. Results showed that brown-marbled grouper exhibited the highest survival rate (82.5 %) and growth performance, achieving the most significant final length (12.32 cm) and body weight (14.02 g). However, it also had the highest feed conversion ratio (FCR = 2.81), indicating lower feed efficiency. Leopard coral grouper demonstrated a balanced growth rate with the lowest FCR (2.00), making it the most cost-effective feed utilization. Financial analysis revealed that leopard coral grouper had the highest profitability, with the most significant net present value (NPV = US$ 65,067) and internal rate of return (IRR = 91.70 %), along with the shortest payback period (1.45 years). Sensitivity analysis indicated that feed costs and market price fluctuations significantly influenced financial viability. The research's findings show that leopard coral grouper is the most profitable species for nursery production, having the highest income, profit, net present value, and IRR. However, brown-marbled grouper has the lowest production cost per unit, making it a feasible option for budget-conscious farmers. While brown-marbled grouper grew the fastest in terms of length and weight, leopard coral grouper maintained competitive growth performance while also having the highest market value, making it the most economically advantageous option. To improve grouper farming's financial resilience, future initiatives should maximize feed efficiency, minimize mortality rates, and employ sustainable aquaculture practices.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"43 ","pages":"Article 102923"},"PeriodicalIF":3.2,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144297949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of different carbohydrate levels on glycolipid metabolism and liver health of channel catfish (Ictalurus punctatus)","authors":"Xiaochen Ma ,&nbsp;Qisheng Lu ,&nbsp;Guoli Han ,&nbsp;Jinglu Jia ,&nbsp;Jingyue Cao ,&nbsp;Haokun Liu ,&nbsp;Junyan Jin ,&nbsp;Zhimin Zhang ,&nbsp;Yunxia Yang ,&nbsp;Xiaoming Zhu ,&nbsp;Dong Han ,&nbsp;Shouqi Xie","doi":"10.1016/j.aqrep.2025.102921","DOIUrl":"10.1016/j.aqrep.2025.102921","url":null,"abstract":"<div><div>This study evaluated the effects of dietary carbohydrate levels on glycolipid metabolism and liver health of channel catfish. Six isonitrogenous and isolipidic diets with corn starch contents of 10 % (C10), 15 % (C15), 20 % (C20), 25 % (C25), 30 % (C30) and 35 % (C35) were set up. After 8-week feeding trial, there was no significant difference in the growth performance of all groups (<em>P</em> &gt; 0.05). The study showed that C30 and C35 groups significantly increased plasma glucose levels, activated the expression of glycolysis-related genes (<em>gk</em>, <em>hk</em>, <em>pklr</em>, <em>pfk</em>) and mitochondrial oxidative phosphorylation related genes (<em>ndufa1</em>, <em>sdhb</em>, <em>sdha</em>, <em>cytb-c1</em>, <em>cox</em>, <em>atp5fa1</em>, <em>atp5f1b</em>), and repressed the expression of gluconeogenesis-related genes (<em>pc</em>, <em>fbp</em>, <em>pepck</em>, <em>g6</em>p) in the channel catfish (<em>P</em> &lt; 0.05). The upregulation of glycogen synthesis-related genes (<em>ppp1r3g</em>, <em>ppp1r3c</em>, <em>ppp1r3b</em>, <em>gys, gnl, ugp2a, ugp2b</em>) contributed to a significant increase in hepatic glycogen content. In addition, C30 and C35 groups significantly increased the expression of pentose phosphate pathway related genes (<em>g6pd</em>, <em>6gpd</em>), while C10 to C25 groups glucose metabolism-related pathways did not exhibit significant differences. Hepatic triglyceride and cholesterol results showed that C30 and C35 groups induced excessive lipogenesis, activated the expression of lipogenesis-related genes (<em>acc</em>, <em>acly</em>, <em>vldlr</em>) and cholesterol synthesis-related genes (<em>srebf2</em>, <em>hmgcr</em>, <em>sqle</em>). In C30 and C35 groups also increased the hepatic ALT, AST and MDA levels, activated the expression of injury-related genes (<em>a-sma</em>, <em>tgf-β</em>), inflammation-related genes (<em>tnf-α</em>, <em>il-1β</em>, <em>il-6</em>) and apoptosis-related genes (<em>baxb</em>, <em>caspase-3</em>, <em>caspase −10</em>) (<em>P</em> &lt; 0.05). C35 group significantly further increased the expression levels of stress genes such as <em>hamp</em>, <em>hsp70</em> and <em>hsp90</em> (<em>P</em> &lt; 0.05), while there were no significant differences in the levels of lactate and cortisol in all groups (<em>P</em> &gt; 0.05). In conclusion, our results suggest that dietary carbohydrate levels less than 30 % will not impair the health and growth performance of the channel catfish.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"43 ","pages":"Article 102921"},"PeriodicalIF":3.2,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144291473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of commercial feeds and natural diets on the culture performance and gut microbiota of Scylla paramamosain","authors":"Xia Zheng ,&nbsp;Dongping Zhou ,&nbsp;Sixiang Wang ,&nbsp;Xiaojing Dong ,&nbsp;Chaoyue Wang ,&nbsp;Zehao Li ,&nbsp;Lei Liu ,&nbsp;Changkao Mu ,&nbsp;Chunlin Wang","doi":"10.1016/j.aqrep.2025.102922","DOIUrl":"10.1016/j.aqrep.2025.102922","url":null,"abstract":"<div><div>In this study, we systematically investigated how different feed combinations affect the growth performance and gut microbiota composition of the mud crab <em>Scylla paramamosain</em> in both indoor cement tanks and outdoor earthen ponds. We employed high-throughput 16S rRNA sequencing to profile the bacterial communities, placing particular emphasis on correlations between feed nutritional components and key microbial taxa. Firmicutes, Proteobacteria, Bacteroidetes, and Cyanobacteria emerged as the predominant phyla. Crabs fed locally sourced natural feeds from Dongying showed relatively minor shifts in community structure, whereas those receiving artificial formulated feed exhibited significant divergences, as evidenced by principal coordinate analysis (PCoA) and non-metric multidimensional scaling (NMDS). In indoor cement tank trials, no significant differences were found in Chao1 among groups. However, the combination of <em>Mactra chinensis</em> + <em>Synechogobius hasta</em> + <em>Trachypenaeus curvirostris</em> group significantly elevated Pielou_e and Shannon diversity indices (<em>P</em> &lt; 0.05), while the Simpson index was higher in both this group and the <em>S. hasta</em> + <em>T. curvirostris</em> + corn + soybean meal combination (<em>P</em> &lt; 0.05). Similar trends were observed in outdoor earthen pond trials, where the <em>M. chinensis</em> + <em>S. hasta</em> + <em>T. curvirostris</em> group and the <em>S. hasta</em> + <em>T. curvirostris</em> + corn + soybean meal group exhibited significantly higher diversity indices (<em>P</em> &lt; 0.05). Moreover, several indicator genera (e.g., OM190, <em>Terrisporobacter</em>, <em>Paraclostridium</em>) were positively correlated with specific nutritional components, implying that balanced feed formulas can promote beneficial bacteria, potentially supporting molting and growth. Collectively, the <em>M. chinensis</em> + <em>S. hasta</em> + <em>T. curvirostris</em> feed combination yielded the most favorable results, highlighting its potential for enhancing both crab growth and gut microbiota diversity. These findings advance our understanding of how feed composition shapes the gut microbiota in <em>S. paramamosain</em> and offer a theoretical basis for optimizing feed formulations, thereby contributing to sustainable aquaculture in Dongying and similar regions.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"43 ","pages":"Article 102922"},"PeriodicalIF":3.2,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144279951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of carbon budgets and greenhouse gas fluxes in monoculture ponds with milkfish (Chanos chanos) and Taiwanese hard clams (Meretrix taiwanica)","authors":"Zhao-Jun Yong ,&nbsp;Wei-Jen Lin ,&nbsp;Shu-Chiu Hsieh ,&nbsp;Hsing-Juh Lin","doi":"10.1016/j.aqrep.2025.102924","DOIUrl":"10.1016/j.aqrep.2025.102924","url":null,"abstract":"<div><div>Aquaculture plays a vital role in meeting the growing demand for aquatic food. However, research on carbon fluxes in aquaculture ponds remains limited. This study constructed carbon budgets and determined greenhouse gas (GHG) emissions from monoculture ponds of Taiwanese hard clams (<em>Meretrix taiwanica</em>) and milkfish (<em>Chanos chanos</em>). Phytoplankton production constituted the major carbon input pathways in the clam (72–85 %) and fish (41–48 %) ponds. However, respiration offset most of the carbon, which turned the fish ponds into heterotrophic systems that emitted an average of 0.21 g C m⁻² d⁻¹. In contrast, the clam ponds remained autotrophic systems, functioning as a carbon sink with an average uptake of 0.06 g C m⁻² d⁻¹, even when accounting for the CO₂ release by biocalcification. The accumulated sediment significantly contributed to the carbon output pathways in the ponds, accounting for 62–77 % of those in the clam ponds and 23–52 % of those in the fish ponds. An unaccounted pathway of carbon input likely contributed to sediment accumulation in the clam ponds caused by the filter-feeding behavior of clams, which induces resuspension or the production of pseudofeces. On the other hand, feed input mainly contributed to sediment accumulation in the fish pond. Methane flux, biocalcification, water exchange, and rainwater accounted for relatively small portions of both carbon budgets. Based on the 100-year global warming potential (GWP₁₀₀), the GHG emissions during the culture period were 0.46 ± 0.75 g CO₂e m⁻² d⁻¹ in the clam ponds and 1.15 ± 1.15 g CO₂e m⁻² d⁻¹ in the fish ponds. Methane fluxes contribute relatively little to the overall GHG emissions from both ponds, while nitrous oxide fluxes can alter the fish ponds into a greater GHG source.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"43 ","pages":"Article 102924"},"PeriodicalIF":3.2,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144270402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of candidate pheromones and neurotransmitter regulation of agonistic behavior in Macrobrachium rosenbergii","authors":"Hangyu Qi ,&nbsp;Qi Shen ,&nbsp;Jiongying Yu ,&nbsp;Miaoying Cai ,&nbsp;Jianyu Song ,&nbsp;Junjie Wu ,&nbsp;Guoliang Yang ,&nbsp;Shaokui Yi","doi":"10.1016/j.aqrep.2025.102920","DOIUrl":"10.1016/j.aqrep.2025.102920","url":null,"abstract":"<div><div><em>Macrobrachium rosenbergii</em>, a crucial commercial prawn species in China, exhibits agonistic behavior under high-density culture conditions, which negatively impacts their integrity, survival, and growth, causing economic losses. This study utilized liquid chromatography mass spectrometry (LC-MS) to identify and classify the metabolic differences in urine and water mixtures of <em>M. rosenbergii</em> displaying agonistic behavior versus those without. Seventeen compounds, such as 4-Acetamidobutyric acid, 1-oleoyl-2-myristoyl-sn-glycero-3-phosphocholine, N-Acety-L-valine, 11-Dehydrocorticosterone, Sphinganine (Sph t18:0), and Acitretin, were identified as candidate pheromones associated with agonistic behavior, 4-Acetamidobutyric acid and Sph (t18:0) were hypothesized to be particularly significant. Additionally, the roles of serotonin (5-HT) and dopamine (DA) in agonistic behavior among <em>M. rosenbergii</em> males were evaluated. We found that 5-HT levels in hemolymph significantly decreased after fighting (<em>P</em> &lt; 0.05), while DA levels significantly increased (<em>P</em> &lt; 0.05) compared to the control group. The receptor genes of 5-HT and DA (5-HTRs and DARs) were further analyzed. Gene expression analyses of <em>M. rosenbergii</em> before and after agonistic behavior revealed that <em>Mr5-HTR1</em> exhibited significant changes in hemolymph and abdominal ganglia, while <em>Mr5-HTR2</em> varied across five tissues, and <em>MrDAR1</em> and <em>MrDAR2</em> showed significant changes in the brain and abdominal ganglia. To verify the impact of 5-HTRs on the agonistic behavior, 5-HTRs agonist and antagonist were in vivo injected. The results showed that the injection of 5-HTRs agonists significantly inhibited the occurrence of agonistic behavior in <em>M. rosenbergii</em>. This study deepens our understanding of the regulatory mechanisms of crustacean agonistic behavior and provides a theoretical basis for improving the crustacean farming management.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"43 ","pages":"Article 102920"},"PeriodicalIF":3.2,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144241132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}